Journal of Integrative Plant Biology最新文献

{"title":"Efficient gene disruption in polyploid genome by Cas9-Trex2 fusion protein.","authors":"Wenbo Pan, Chunlei Gao, De Niu, Jinghua Cheng, Jiao Zhang, Xiying Yan, Qiang Long, YaoYao Zhu, Wenjing Sun, Qi Xie, Yuehui He, Xing Wang Deng, Huawei Zhang, Jian Li","doi":"10.1111/jipb.13797","DOIUrl":"https://doi.org/10.1111/jipb.13797","url":null,"abstract":"<p><p>The fusion of the exonuclease Trex2 with the Cas9 protein significantly enhanced the efficiency of genome editing in hexaploid common wheat, particularly for the simultaneous editing of multiple favorable alleles within a single generation, thereby facilitating genome editing-assisted breeding in polyploid crops.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How have breeders adapted rice flowering to the growing region?","authors":"Asako Kobayashi,&nbsp;Mao Suganami,&nbsp;Hideki Yoshida,&nbsp;Yoichi Morinaka,&nbsp;Syuto Watanabe,&nbsp;Yoshie Machida,&nbsp;Genki Chaya,&nbsp;Fumihiro Nakaoka,&nbsp;Nobuhito Sato,&nbsp;Kotaro Miura,&nbsp;Makoto Matsuoka","doi":"10.1111/jipb.13785","DOIUrl":"10.1111/jipb.13785","url":null,"abstract":"<p>Flowering time is a crucial rice trait that influences its adaptation to various environments, cropping schedules, and agronomic characteristics. Rice breeders have exploited spontaneous mutations in heading date genes to regulate the flowering time. In the present study, we investigated how breeders in Fukui Prefecture regulated days to heading while developing promising rice varieties. Genome-wide association studies (GWAS) identified <i>Hd1, Hd16</i>, and <i>Hd18</i> as the major genes controlling days to heading in the population. However, we suspected that this highly bred population might exhibit genomic stratification, which could lead to spurious or false correlations in the GWAS. Thus, we also conducted correlation and partial correlation analyses, which uncovered another key heading date gene, <i>Hd17</i>, that GWAS failed to detect because of its linkage disequilibrium with the major effect gene <i>Hd16</i>. Examination of haplotype frequencies across different breeding periods revealed that the early-heading <i>Hd16</i> (<i>Hd16(E)</i>) and late-heading <i>Hd17</i> (<i>Hd17(L)</i>) were increasingly co-selected in the <i>Hd1</i> functional population. Varieties carrying this <i>Hd16(E)/Hd17(L)</i> combination exhibited days to heading in the range of 70–80, which corresponds to the peak temperature and sunshine period and is also optimal for grain quality and yield components in the Fukui environment. The present study highlights that it is imperative to remain vigilant for Type I (false positives) and Type II (false negatives) errors when performing GWAS on highly bred populations and to implement appropriate countermeasures by accounting for gene-by-gene interactions established through the breeding process. We also discuss the effectiveness of <i>Hd16(E)</i>, which is not used outside Japan for subtle days to heading control but is widely used in Japan at certain latitudes.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2736-2753"},"PeriodicalIF":9.3,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11622534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-faced OsNAS3 influences disease resistance via nicotianamine and ethylene","authors":"Kaiwei He,&nbsp;Liting Xu,&nbsp;Qin He,&nbsp;Wei Zhang,&nbsp;Ying Zhang,&nbsp;Xiaobo Zhu,&nbsp;Junjie Yin,&nbsp;Qing Xiong,&nbsp;Qingqing Hou,&nbsp;Yongyan Tang,&nbsp;Min He,&nbsp;Xuewei Chen,&nbsp;Weitao Li","doi":"10.1111/jipb.13788","DOIUrl":"10.1111/jipb.13788","url":null,"abstract":"<p>The loss and gain of <i>OsNAS3</i> function both positively influence plant disease resistance. Overexpression of <i>OsNAS3</i> boosts blast resistance by promoting nicotianamine accumulation, thereby enhancing blast resistance. Conversely, knockout of <i>OsNAS3</i> increases ethylene biosynthesis, also contributing to improved blast resistance.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2581-2585"},"PeriodicalIF":9.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13788","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfur metabolism under stress: Oxidized glutathione inhibits methionine biosynthesis by destabilizing the enzyme cystathionine γ-synthase.","authors":"Yael Hacham, Alex Kaplan, Elad Cohen, Maayan Gal, Rachel Amir","doi":"10.1111/jipb.13799","DOIUrl":"https://doi.org/10.1111/jipb.13799","url":null,"abstract":"<p><p>Cysteine is the precursor for the biosynthesis of glutathione, a key stress-protective metabolite, and methionine, which is imperative for cell growth and protein synthesis. The exact mechanism that governs the routing of cysteine toward glutathione or methionine during stresses remains unclear. Our study reveals that under oxidative stress, methionine and glutathione compete for cysteine and that the increased oxidized glutathione (GSSG) levels under stress hinder methionine biosynthesis. Moreover, we find that inhibition occurs as GSSG binds to and accelerates the degradation of cystathionine γ-synthase, a key enzyme in the methionine synthesis pathway. Consequently, this leads to a reduction in the flux toward methionine-derived metabolites and redirects cysteine utilization toward glutathione, thereby enhancing plant protection. Our study suggests a novel regulatory feedback loop involving glutathione, methionine, and cysteine, shedding light on the plant stress response and the adaptive rerouting of cysteine. These findings offer new insights into the intricate balance of growth and protection in plants and its impact on their nutritional value due to low methionine levels under stress.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TaWRKY55-TaPLATZ2 module negatively regulate saline-alkali stress tolerance in wheat.","authors":"Lin Wei, Xinman Ren, Lumin Qin, Rong Zhang, Minghan Cui, Guangmin Xia, Shuwei Liu","doi":"10.1111/jipb.13793","DOIUrl":"10.1111/jipb.13793","url":null,"abstract":"<p><p>Saline-alkaline soils are a major environmental problem that limit plant growth and crop productivity. Plasma membrane H⁺-ATPases and the salt overly sensitive (SOS) signaling pathway play important roles in plant responses to saline-alkali stress. However, little is known about the functional genes and mechanisms regulating the transcription of H⁺-ATPases and SOS pathway genes under saline-alkali stress. In the present study, we identified that the plant AT-rich sequence and zinc-binding (TaPLATZ2) transcription factor are involved in wheat response to saline-alkali stress by directly suppressing the expression of TaHA2/TaSOS3. The knockdown of TaPLATZ2 enhances salt and alkali stress tolerance, while overexpression of TaPLATZ2 leads to salt and alkali stress sensitivity in wheat. In addition, TaWRKY55 directly upregulated the expression of TaPLATZ2 during saline-alkali stress. Through knockdown and overexpression of TaWRKY55 in wheat, TaWRKY55 was shown to negatively modulate salt and alkali stress tolerance. Genetic analyses confirmed that TaPLATZ2 functions downstream of TaWRKY55 in response to salt and alkaline stresses. These findings provide a TaWRKY55-TaPLATZ2-TaHA2/TaSOS3 regulatory module that regulates wheat responses to saline-alkali stress.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":" ","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The LpHsfA2-molecular module confers thermotolerance via fine tuning of its transcription in perennial ryegrass (Lolium perenne L.)","authors":"Guangjing Ma,&nbsp;Zhihao Liu,&nbsp;Shurui Song,&nbsp;Jing Gao,&nbsp;Shujie Liao,&nbsp;Shilong Cao,&nbsp;Yan Xie,&nbsp;Liwen Cao,&nbsp;Longxing Hu,&nbsp;Haichun Jing,&nbsp;Liang Chen","doi":"10.1111/jipb.13789","DOIUrl":"10.1111/jipb.13789","url":null,"abstract":"<p>Temperature sensitivity and tolerance play a key role in plant survival and production. Perennial ryegrass (<i>Lolium perenne</i> L.), widely cultivated in cool-season for forage supply and turfgrass, is extremely susceptible to high temperatures, therefore serving as an excellent grass for dissecting the genomic and genetic basis of high-temperature adaptation. In this study, expression analysis revealed that <i>LpHsfA2</i>, an important gene associated with high-temperature tolerance in perennial ryegrass, is rapidly and substantially induced under heat stress. Additionally, heat-tolerant varieties consistently display elevated expression levels of <i>LpHsfA2</i> compared with heat-sensitive ones. Comparative haplotype analysis of the <i>LpHsfA2</i> promoter indicated an uneven distribution of two haplotypes (<i>HsfA2</i>^Hap1 and <i>HsfA2</i>^Hap2) across varieties with differing heat tolerance. Specifically, the <i>HsfA2</i>^Hap1 allele is predominantly present in heat-tolerant varieties, while the <i>HsfA2</i>^Hap2 allele exhibits the opposite pattern. Overexpression of <i>LpHsfA2</i> confers enhanced thermotolerance, whereas silencing of <i>LpHsfA2</i> compromises heat tolerance. Furthermore, <i>LpHsfA2</i> orchestrates its protective effects by directly binding to the promoters of <i>LpHSP18.2</i> and <i>LpAPX1</i> to activate their expression, preventing the non-specific misfolding of intracellular protein and the accumulation of reactive oxygen species in cells. Additionally, LpHsfA4 and LpHsfA5 were shown to engage directly with the promoter of <i>LpHsfA2</i>, upregulating its expression as well as the expression of <i>LpHSP18.2</i> and <i>LpAPX1</i>, thus contributing to enhanced heat tolerance. Markedly, LpHsfA2 possesses autoregulatory ability by directly binding to its own promoter to modulate the self-transcription. Based on these findings, we propose a model for modulating the thermotolerance of perennial ryegrass by precisely regulating the expression of LpHsfA2. Collectively, these findings provide a scientific basis for the development of thermotolerant perennial ryegrass cultivars.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2346-2361"},"PeriodicalIF":9.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13789","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue information page","authors":"","doi":"10.1111/jipb.13528","DOIUrl":"https://doi.org/10.1111/jipb.13528","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 10","pages":"2077-2078"},"PeriodicalIF":9.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13528","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover Image:","authors":"","doi":"10.1111/jipb.13529","DOIUrl":"https://doi.org/10.1111/jipb.13529","url":null,"abstract":"<p>Pineapple, an important tropical herbaceous fruit tree, is renowned for its juicy composite fruits and distinctive aroma. Its extensive evolutionary history, primarily driven by vegetative propagation, has led to a highly heterozygous genome that has been difficult to fully resolve. Here, Feng et al. (pages 2208–2225) have successfully assembled the first telomere-to-telomere genome of pineapple, accompanied by a meticulously curated, highquality gene structure annotation. These comprehensive genomic resources provide a complete map for postgenomic research and breeding efforts in pineapple. The cover image features a flowering hybrid F1 plant, the result of a cross between BL and LY, two pineapple varieties used in the study.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 10","pages":"C1"},"PeriodicalIF":9.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sucrose induces flowering by degradation of the floral repressor Ghd7 via K48-linked polyubiquitination in rice","authors":"Lae-Hyeon Cho,&nbsp;Jinmi Yoon,&nbsp;Gibeom Baek,&nbsp;Win Tun,&nbsp;Hyeok Chan Kwon,&nbsp;Dae-Woo Lee,&nbsp;Seok-Hyun Choi,&nbsp;Yang-Seok Lee,&nbsp;Jong-Seong Jeon,&nbsp;Gynheung An","doi":"10.1111/jipb.13790","DOIUrl":"10.1111/jipb.13790","url":null,"abstract":"<p>Sucrose functions as a signaling molecule in several metabolic pathways as well as in various developmental processes. However, the molecular mechanisms by which sucrose regulates these processes remain largely unknown. In the present study, we demonstrate that sucrose promotes flowering by mediating the stability of a regulatory protein that represses flowering in rice. Exogenous application of sucrose promoted flowering by inducing florigen gene expression. Reduction of sucrose levels in the phloem through genetic modifications, such as the overexpression of the vacuolar invertase <i>OsVIN2</i> or the mutation of <i>OsSUT2</i>, a sucrose transporter, delayed flowering. Analysis of relative transcript levels of floral regulatory genes showed that sucrose activated <i>Ehd1</i> upstream of the florigen, with no significant effect on the expression of other upstream genes. Examination of protein stability after sucrose treatment of major floral repressors revealed that the Ghd7 protein was specifically degraded. The Ghd7 protein interacted with the E3 ligase IPA INTERACTING PROTEIN1 (IPI1), and sucrose-induced K48-linked polyubiquitination of Ghd7 via IPI1, leading to protein degradation. Mutants defective in IPI1 delayed flowering, confirming its role in modulating proteins involved in flowering. We conclude that sucrose acts as a signaling molecule to induce flowering by promoting Ghd7 degradation via IPI1.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 12","pages":"2683-2700"},"PeriodicalIF":9.3,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11622536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why is pollen in Camellia oleifera inedible to honeybees?","authors":"Jannathan Mamut,&nbsp;Wei-Bing Zhang,&nbsp;Lu-Lu Tang","doi":"10.1111/jipb.13787","DOIUrl":"10.1111/jipb.13787","url":null,"abstract":"<p>This Commentary examines a recent study that addressed a long-standing controversy: Is the lethal effect of Tea-oil Camellia on honeybee larvae due to nectar or pollen toxicity? Flowers of <i>Camellia oleifera</i> are adapting to bird pollination, evolving ‘anti-bee’ traits such as theasaponin-containing pollen, which is toxic to bee larvae.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2307-2309"},"PeriodicalIF":9.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13787","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}