Doklady Biochemistry and Biophysics最新文献

{"title":"Effect of Low Doses of Accelerated Carbon Ions with Energy of 450 MeV/Nucleon on Adaptive Response and Genetic Instability in Two Generations of Mice.","authors":"V E Balakin, O M Rozanova, T A Belyakova, E N Smirnova, N S Strelnikova","doi":"10.1134/S1607672925601829","DOIUrl":"https://doi.org/10.1134/S1607672925601829","url":null,"abstract":"<p><p>The patterns of radiation adaptive response (RAR) induction and transgenerational genomic instability in mice following exposure to carbon ions (¹²C) with a linear energy transfer (LET) of ~39 keV/μm and to X‑rays with a LET of ~2 keV/μm at a dose of 10 cGy were studied. Low doses of ¹²C, as well as X‑rays, induce RAR, the magnitude of which depends on the quality of the challenge radiation. In the first- and second- generation offspring of males irradiated ¹²C at a dose of 10 cGy, an increased spontaneous level of cytogenetic damage and the absence of RAR in the first generation were detected, in contrast to the offspring of males after irradiation with X‑rays.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uptake and Retention of Laser-synthesized Boron Nanoparticles in Tumor Cells and Fibroblasts.","authors":"A I Kasatova, K S Kuzmina, P A Kotelnikova, E V Barmina, K O Aiyyzhy, A A Laktionov, D I Tselikov, I V Sozaev, I V Lunyov, S M Klimentov, M S Grigoryeva, D S Petrunya, E L Zavjalov, S Yu Taskaev, S M Deyev, I N Zavestovskaya","doi":"10.1134/S1607672925601775","DOIUrl":"https://doi.org/10.1134/S1607672925601775","url":null,"abstract":"<p><p>Boron Neutron Capture Therapy (BNCT) is one of the innovative methods for treating oncological diseases. Its selectivity is based on the targeted delivery of the boron-10 isotope to tumor cells, followed by neutron irradiation, the ¹⁰B(n, α)⁷Li reaction occurs with a local release of 2.79 MeV of energy. Budding boron delivery agents are nanoscale systems. This study evaluated in vitro cytotoxicity, accumulation, and retention of elemental boron nanoparticles, synthesized by laser ablation and laser fragmentation, in U87 and BT474 tumor cells and BJ-5ta fibroblasts. It was shown that both types of nanoparticles exhibit low cytotoxicity at therapeutically relevant concentrations. Boron accumulation was maximal after 24 h of incubation and was significantly higher in tumor cells, especially in the BT474 cell line, compared to fibroblasts. The obtained data indicate the promise of these nanoparticles as boron delivery agents for BNCT.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between the rs1052133 Polymorphism of the OGG1 Gene and the Risk of Malignant Neoplasm Development in People Chronically Exposed to Radiation.","authors":"M A Yanishevskaya, E A Blinova, A V Korechenkova, A V Akleyev","doi":"10.1134/S1607672926600077","DOIUrl":"https://doi.org/10.1134/S1607672926600077","url":null,"abstract":"<p><p>Genetic predisposition without doubt is one of the risk factors of cancer initiation. It is known that single nucleotide polymorphisms (SNP) of genes that maintain the genome stability, including SNP of DNA repair, may contribute to the initiation of carcinogenesis. Single-nucleotide polymorphisms of genes that support genome stability, including SNP of DNA repair genes, can contribute to cancer initiation. Polymorphism of the excision repair gene OGG1 causes interest of leading scientific groups from various countries. It is assumed that there is relationship between the rs1052133 polymorphism in the gene and predisposition to cancer initiation. The objective of this study was to establish association between rs1052133 polymorphism of base excision repair gene OGG1 and the risk of cancer initiation in people chronically exposed to ionizing radiation. Residents (888 people) of the Techa riverside settlements, chronically exposed to low or medium dose radiation from the Techa River and the East-Urals Radioactive Trace were included in the study. The study allowed researchers to establish that exposed to chronic radiation people, carriers of the rs1052133*G allele, have increased risk of malignant neoplasm initiation: OR = 1.38; 95% CI [1.05-1.83], p = 0.023. The multifactorial synergistic interactions between the dose to the red bone marrow and the rs1052133 polymorphism of the OGG1 gene was found: Testing Balanced Accuracy (TBA) = 0.56; Cross Validation Consistency (CVC) = 10/10; p = 0.01). The study found that the rs1052133 polymorphism may be considered as genetical marker of risk of cancer initiation in people chronically exposed to radiation with doses ranged from 0.74 to 3507.07 mGy (average 523.10 ± 33.89 mGy). It was found that the presence of the rs1052133*G in combination with radiation exposure can modify the risk of solid cancers initiation, as it is indicated by the synergistic relationship between the SNP and the radiation dose.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Bacterial Bioluminescent System as a Tool for Monitoring Biogenic Aldehydes.","authors":"A S Shcheglov, S A Lukyanov, I V Yampolsky","doi":"10.1134/S1607672926600041","DOIUrl":"https://doi.org/10.1134/S1607672926600041","url":null,"abstract":"<p><p>Aliphatic aldehydes are involved in many important biological processes, but detection of them at low concentrations requires expensive laboratory equipment and labor-intensive analytical methods. Typically, chromatographic and chromatograph mass spectrometric methods are used for this purpose. Therefore, developing a simple method for detecting aldehydes at low concentrations is a pressing scientific and technical challenge. We have demonstrated that a bacterial bioluminescent system can be effectively used for the semiquantitative determination of biogenic aldehydes in vitro at nanomolar concentrations. Furthermore, this system holds promise for detecting biogenic aldehydes in vivo.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphism of hOGG1 Gene and Susceptibility to Malignant Neoplasms in People Affected by Long-Term Low-Dose-Rate Exposure.","authors":"M A Yanishevskaya, E A Blinova, E A Shishkina, A V Akleyev","doi":"10.1134/S1607672926600065","DOIUrl":"https://doi.org/10.1134/S1607672926600065","url":null,"abstract":"<p><p>In the previous study [1], we showed an increased risk of malignant neoplasms in carriers of the minor allele rs1052133*G of the hOGG1 gene who were affected by chronic radiation exposure at a wide range of doses (up to 3507 mGy to the red bone marrow at the Techa River (Southern Urals). The objective of the present study was to assess the contribution of radiation factor to the risk of malignant neoplasm development in persons chronically exposed at the Techa River. For this purpose, we analyzed the background level of genetically determined risk in the general population of unexposed people on the basis of meta-analysis of the world literature data on the search for the association of rs1052133 of the hOGG1 gene with the risk of malignant neoplasm development. At the final stage, the results of the meta-analysis were compared with data on exposed people. The study found that unexposed and exposed carriers of the rs1052133*G allele had a comparable increased risk of developing malignant neoplasms, odds ratio OR = 1.20; 95% confidence interval [1.06-1.35], p = 0.01 and odds ratio OR = 1.38; 95% confidence interval [1.05-1.83], p = 0.023, respectively.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an Approach for Parasegment-Specific Labeling of Nuclei in Drosophila melanogaster Embryos.","authors":"I O Deriglazova, O V Kyrchanova, P G Georgiev, O G Maksimenko","doi":"10.1134/S1607672925601805","DOIUrl":"https://doi.org/10.1134/S1607672925601805","url":null,"abstract":"<p><p>Effective approaches for selecting nuclei in which a gene of interest is in an active state are necessary for studying spatial organization and gene expression regulation. In this work, a novel two-component genetic system was created for the parasegment-specific labeling of nuclei in which the regulatory domain iab-5, which stimulates the homeotic gene Abd-B, is active. The system is based on the integration of a transgene expressing the yeast GAL4 activator under the control of the minimal hsp70 gene promoter into the iab-5 domain, near the early embryonic enhancer. As a result, the iab-5 enhancer induces specific expression of GAL4, which strongly amplifies the expression of the mScarlet fluorescent protein, allowing for the efficient selection of labeled nuclei in which the iab-5 domain is activated. This approach can be used for the selection of target nuclei in which any regulatory element or gene of interest is in an active or repressed state.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Model for the Transmembrane Heterocomplex of Hemagglutinin HA and Ionic Channel M2 Proteins in Influenza A Virus.","authors":"O P Zhirnov, A I Chernyshova","doi":"10.1134/S1607672925601878","DOIUrl":"https://doi.org/10.1134/S1607672925601878","url":null,"abstract":"<p><p>The lipid envelope of the influenza A virus contains two major types of protein spikes formed by the transmembrane hemagglutinin trimers (HA, MW 80 kDa) and neuraminidase tetramers (NA, MW 55 kDa), in quantities of 500 and 120, respectively. The third transmembrane protein, M2 (MW 14 kDa), forms tetramers of ion channels in quantities of about 10-20 per virion. Modeling of the molecular structure using the AlphaFold software tool showed a novel model for a heterocomplex of HA0-M2 proteins, in which the M2 tetramer located inside the HA0 trimer like a \"matryoshka doll.\" Similar models of the HA0-M2 heterocomplex were obtained for the A/Aichi/2/68 (H3N2) and A/WSN/33 (H1N1) viruses. The resulting HA0-M2 heterocomplex possessed a high structural complementarity of the macromolecular interfaces (ipTM = 0.65), had no structural clashes of atoms in the molecular interfaces (clash score = 0.0), and exhibited stable and reliable intermolecular topology with a high ranking score of 0.79. The constructed model allows to explain the phenomenon of blocking the function of M2 ion channels by the rigid conformation of the uncleaved HA0 protein and, in contrast, the activation of M2 channels after (i) specific point proteolysis of HA0 into HA1 (55 kDa) and HA2 (25 kDa) subunits and (ii) exposure to acidic pH of 4.0-5.5, leading to the disclosure of the 3D structure of the HA1/2 molecule and the opening of the M2 channel.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modular Nanotransporters Containing Anti-PSMA Nanobodies Are Able to Penetrate into the Nuclei of Prostate Cancer Cells.","authors":"Y V Khramtsov, A V Ulasov, T A Slastnikova, T N Lupanova, A A Rosenkranz, G P Georgiev, A S Sobolev","doi":"10.1134/S1607672925601908","DOIUrl":"https://doi.org/10.1134/S1607672925601908","url":null,"abstract":"<p><p>Polypeptide modular nanotransporters (MNTs) were engineered as a targeted delivery platform for prostate cancer cells. The constructs integrate a ligand module to facilitate specific cellular binding and internalization, and a nuclear localization signal (NLS) to enable subsequent nuclear translocation. Two distinct ligand modules were utilized: (a) a nanobody targeting prostate-specific membrane antigen (anti-PSMA) and (b) a gastrin-releasing peptide (GRP) fragment targeting the GRP receptor (GRPR). It was shown that all modules within MNT-antiPSMA and MNT-GRP retained their functional properties. The MNT-antiPSMA construct demonstrated the capacity to accumulate specifically in the nuclei of prostate cancer cells with both low and high PSMA expression. Conversely, MNT-GRP exhibited selective nuclear entry exclusively in cells characterized by high GRPR expression.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Olokizumab in Treating Comorbid Depression in Patients with Rheumatoid Arthritis: Results of a Single-Center Randomized Controlled Trial.","authors":"T A Lisitsyna, A A Abramkin, D Yu Veltishchev, A B Borisova, E L Nasonov","doi":"10.1134/S1607672925700231","DOIUrl":"https://doi.org/10.1134/S1607672925700231","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Interleukin (IL)-6 plays an important role in the pathogenesis of comorbid rheumatoid arthritis (RA) depression. IL-6 inhibitors used to treat patients with RA may also have an antidepressant effect. THE OBJECTIVE-: of this study is to evaluate the effectiveness of 24-week IL-6 inhibitor therapy with olokizumab (OKZ) in combination with or without psychopharmacotherapy (PPT) in patients with moderate to high RA activity.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;-A total of 125 patients with RA were included, 102 (81.6%) of them were women. The average age of the patients was 48.5 ± 12.6 years; the majority of the patients (86.4%) had high RA activity and had shown ineffectiveness with stable 12-week therapy using conventional synthetic disease modifying antirheumatic drugs (csDMARDs). Additionally, 34 (27.2%) patients had shown inefficiency with one or more biological DMARDs. According to the International Classification of Diseases, 10th revision (ICD-10), a psychiatrist diagnosed varying severity of depression (chronic or recurrent) in all patients during a semi-structured interview. At week 0, all patients were randomized using sequential numbers in a 2:2:1 ratio into one of three groups: in group 1, patients received csDMARDs + OKZ 64 mg subcutaneously once every 4 weeks (q4w) (n = 49); in group 2, patients received csDMARDs + OKZ 64 mg subcutaneously q4w along with PPT (n = 51); in group 3, patients received csDMARDs + PPT (n = 25). The study duration was 24 weeks. The severity of depression was assessed using the PHQ-9 (Patient Health Questionnaire 9) and MADRS (Montgomery-Asberg Depression Rating Scale) scales, and anxiety was assessed using the HAM-A (Hamilton Anxiety Rating Scale) scale. Projective experimental psychological techniques were also used.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;-After 12 and 24 weeks of therapy, a significant decrease in the severity of depression and anxiety was observed in all patients' groups. However, the differences between the final and initial values of the scales filled in by a psychiatrist were statistically significantly greater (p &lt; 0.001) in the groups of patients receiving PPT: in group 2 (ΔMADRS&lt;sub&gt;24-0&lt;/sub&gt; = -20.2 ± 6.57; ΔHAM-A&lt;sub&gt;24-0&lt;/sub&gt; = -13.2 ± 5.68) and group 3 (ΔMADRS&lt;sub&gt;24-0&lt;/sub&gt; = -17.8 ± 4.73; ΔHAM-A&lt;sub&gt;24-0&lt;/sub&gt; = -13.4 ± 4.41), compared with the group 1 (ΔMADRS&lt;sub&gt;24-0&lt;/sub&gt; = -5.42 ± 7.14; ΔHAM-A&lt;sub&gt;24-0&lt;/sub&gt; = -4.58 ± 6.80). There were no significant differences between the groups according to the PHQ-9 depression questionnaire (in group 1, ΔPHQ-9&lt;sub&gt;24-0&lt;/sub&gt; = -4.89 ± 4.87; in group 2, ΔPHQ-9&lt;sub&gt;24-0&lt;/sub&gt; = -6.73 ± 4.97; in group 3, ΔPHQ-9&lt;sub&gt;24-0&lt;/sub&gt; = -7.26 ± 5.58, respectively), despite a greater decrease in the severity of depression observed in the groups with PPT. According to a semi-structured interview with a psychiatrist and in accordance with the criteria of ICD-10 the proportion of patients without depression 24 weeks after the start of therap","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Safety of Long-term Administration of Various Doses of Colchicine in Patients with Gout.","authors":"M S Eliseev, M N Chikina, Y I Kuzmina, E V Panina, E L Nasonov","doi":"10.1134/S1607672925700267","DOIUrl":"https://doi.org/10.1134/S1607672925700267","url":null,"abstract":"<p><p>to compare the efficacy and safety of different doses of colchicine (0.5 mg/day vs. 1.0 mg/day) used for the prevention of arthritis attacks in gout patients during the initiation of urate-lowering therapy.</p><p><strong>Materials and methods: </strong>-The study included 96 patients diagnosed with gout. Patients were randomized into three groups: those receiving colchicine 0.5 mg/day, those receiving colchicine 1 mg/day, and those without anti-inflammatory therapy. All participants were prescribed febuxostat 80 mg/day. The duration of the observation period was 6 months. The frequency and severity of arthritis attacks, as well as the incidence of adverse events, were compared.</p><p><strong>Results: </strong>-Patients who did not receive colchicine experienced arthritis attacks more frequently compared to those receiving colchicine 0.5 mg/day (p = 0.03) and 1 mg/day (p = 0.007). In the groups receiving colchicine 0.5 and 1.0 mg/day, the frequency of attacks did not differ significantly (p = 0.6), nor did the proportion of patients who did not experience arthritis attacks (18 (56%) and 22 (69%), respectively, p = 0.3). Among the patients not taking colchicine, 9 (28%) did not develop arthritis attacks (p = 0.02 compared to those taking 0.5 mg/day and p = 0.001 for 1 mg/day). The administration of colchicine 1 mg/day (but not 0.5 mg/day) was associated with lower pain intensity on the visual analog scale (VAS) during arthritis attacks compared to the non-therapy group (p = 0.04). The frequency of adverse events was comparable across the groups.</p><p><strong>Conclusions: </strong>-The use of colchicine 0.5 mg/day for the prevention of arthritis attacks in gout patients is justified due to the absence of differences in efficacy compared to the higher dose. However, prescribing colchicine 1 mg/day also demonstrates a good safety profile and may be considered for the prevention of arthritis attacks.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}