Doklady Biochemistry and Biophysics最新文献

Preparation of 6-Azido-2-Chloropurine-2'-Deoxyriboside by Enzymatic Transglycosylation Reaction Catalyzed by Lactobacillus leichmannii Type II Nucleoside Deoxyribosyltransferase. 利氏乳杆菌II型核苷脱氧核糖基转移酶催化的酶转糖基化反应制备6-叠氮-2-氯嘌呤-2′-脱氧核糖苷

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-21 DOI: 10.1134/S160767292560040X

C S Alexeev, M A Konkina, N N Kurochkin, M S Drenichev

引用次数: 0

Effect of a Comparative Analysis of Different Annotations of the Oryza sativa Rice Genome for In Silico Verification of Predicted Promoter Sequences. 水稻基因组不同注释比对分析对启动子序列预测的影响

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-21 DOI: 10.1134/S1607672925600332

A N Bubnova, I V Yakovleva, A M Kamionskaya

引用次数: 0

Protein Complementation Approach for Quantitative and Qualitative Analysis of hTERP in Cell. 细胞中hTERP定量和定性分析的蛋白质互补方法。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-21 DOI: 10.1134/S1607672925600514

M A Shamonova, M S Koriagina, V L Shliapina, O A Dontsova, M P Rubtsova

引用次数: 0

HDAC3 Regulates Ferroptosis via Nrf2-GPX4 Signaling in Colorectal Cancer Cells. HDAC3通过Nrf2-GPX4信号通路调控结直肠癌细胞铁下垂。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-14 DOI: 10.1134/S1607672925600496

Wei Jin, Jue-Jue Wang, Yan-Fei Feng, Bing Chen, Zhao-Hua Hu

{"title":"HDAC3 Regulates Ferroptosis via Nrf2-GPX4 Signaling in Colorectal Cancer Cells.","authors":"Wei Jin, Jue-Jue Wang, Yan-Fei Feng, Bing Chen, Zhao-Hua Hu","doi":"10.1134/S1607672925600496","DOIUrl":"https://doi.org/10.1134/S1607672925600496","url":null,"abstract":"Ferroptosis, an iron-dependent form of regulated cell death, represents an emerging therapeutic vulnerability in colorectal cancer (CRC). However, the epigenetic mechanisms controlling ferroptosis sensitivity in CRC remain poorly understood. Here, we identify histone deacetylase 3 (HDAC3) as a pivotal epigenetic suppressor of ferroptosis. Both pharmacological inhibition and genetic knockdown of HDAC3 significantly enhanced ferroptosis sensitivity, as evidenced by elevated intracellular ferrous iron (Fe2+) and lipid peroxidation. Mechanistically, inhibition of HDAC3 reduced the expression of nuclear factor erythroid 2-related factor 2 (NRF2), a master antioxidant transcription factor, thereby leading to downregulation of glutathione peroxidase 4 (GPX4), a central ferroptosis defense gene. Notably, NRF2 knockdown abolished GPX4 downregulation by HDAC3 inhibition, whereas GPX4 overexpression rescued the ferroptotic phenotype caused by HDAC3 depletion. Collectively, these findings define an HDAC3-NRF2-GPX4 axis that suppresses ferroptosis in CRC, and highlight HDAC3 as a potential therapeutic target for ferroptosis-based cancer treatment.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flowering Initiation Genes Determine the Node for Inflorence-Ear in Zea Mays L. 玉米开花起始基因决定颖穗节点。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-14 DOI: 10.1134/S1607672925600320

D Kh Arkhestova, E Z Kochieva, A V Shchennikova

引用次数: 0

Association of Diurnal Dynamics of Myocardial Infarction with Distribution of Spikes in TiNi Detector Readings. 心肌梗死的日动态与TiNi检测器读数的尖峰分布的关系。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-14 DOI: 10.1134/S1607672925600423

G Ts Dambaev, V Ya Erofeev, A A Garganeeva, E A Kuzheleva, S A Okrugin

引用次数: 0

Biphasic Effects of bFGF Blockade on Lung Cancer Cell Invasion. 阻断bFGF对肺癌细胞侵袭的双相作用。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-14 DOI: 10.1134/S1607672925600198

Jiaqi Li, Zhiyong Wang, Yanxin Lu, Qiang Xia, Pei Wei

引用次数: 0

Antitumor Effect of Helium Ions with Energy of 320 MeV/Ion during Irradiation of Ehrlich Ascites Carcinoma Cells Ex Vivo 能量为320 MeV/离子的氦离子辐照埃利希腹水癌细胞的体外抗肿瘤作用。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-06-02 DOI: 10.1134/S1607672925600356

V. E. Balakin, N. S. Strelnikova, O. M. Rozanova, E. N. Smirnova, T. A. Belyakova, A. E. Shemyakov

引用次数: 0

Evidence for Functionality of Transmembrane Domains of Predicted Non-Canonical Plant Phosphotransmitters 预测非典型植物磷酸化递质跨膜结构域功能的证据。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-06-02 DOI: 10.1134/S1607672925600381

S. N. Lomin, E. M. Savelieva, A. S. Elanskaya, D. V. Arkhipov, G. A. Romanov

{"title":"Evidence for Functionality of Transmembrane Domains of Predicted Non-Canonical Plant Phosphotransmitters","authors":"S. N. Lomin, E. M. Savelieva, A. S. Elanskaya, D. V. Arkhipov, G. A. Romanov","doi":"10.1134/S1607672925600381","DOIUrl":"10.1134/S1607672925600381","url":null,"abstract":"Bioinformatic methods have been used to predict a new subclass of proteins among plant phosphotransmitters involved in the signaling system of multistep phosphorelay. In contrast to the canonical soluble nucleocytosolic forms, the found noncanonical phosphotransmitter sequences, belonging to a wide range of plant taxa, potentially contain transmembrane domains. This suggests localization of such proteins on cell membranes and, therefore, a different function in signaling than that of canonical phosphotransmitters. We tested the functionality of the transmembrane domain of the phosphotransmitter using the protein of the tea plant Camellia sinensis. The membrane localization of the transiently expressed recombinant phosphotransmitter with this domain was confirmed by microscopy and immunoblotting. Thus, this is the first study to obtain experimental evidence for the existence of membrane-bound plant phosphotransmitters with as yet unknown functions. These data suggest the presence of a noncanonical membrane branch of signal transduction in the multistep phosphorelay system in plants.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"522 1","pages":"309 - 314"},"PeriodicalIF":0.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Vβ-Segment Diversity of T-cell Receptor in Techa Riverside Residents Chronically Exposed to Radiation in the Long-Term Period 长期长期受辐射的Techa河畔居民t细胞受体v β段多样性分析

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-05-11 DOI: 10.1134/S1607672925700164

A. I. Kotikova, E. A. Blinova, A. V. Akleyev

{"title":"Analysis of Vβ-Segment Diversity of T-cell Receptor in Techa Riverside Residents Chronically Exposed to Radiation in the Long-Term Period","authors":"A. I. Kotikova, E. A. Blinova, A. V. Akleyev","doi":"10.1134/S1607672925700164","DOIUrl":"10.1134/S1607672925700164","url":null,"abstract":"To study the repertoire of the T-cell receptor in chronically exposed persons in the long-term period. The study involved 48 people who were divided into two groups: a group of exposed persons (31 individuals with the mean accumulated dose to red bone marrow (RBM) of 981 ± 130 mGy) and a comparison group (17 individuals, the mean accumulated dose to RBM of 25.3 ± 5.91 mGy). The study groups did not differ significantly in age, gender, and ethnicity. The repertoire of Vβ-segments of the T-cell receptor of the peripheral blood T-lymphocytes of exposed persons was analyzed by flow cytometry method. 24 Vβ-segments of the T-cell receptor were studied. Statistical processing of the obtained data was carried out using the Wilcoxon signed-rank test, and a direct description of Vβ-segment repertoire of the T-cell receptor was performed using the Lorenz curve and the Gini-TCR index. The study revealed a statistically significant increase in the number of Vβ3 and Vβ5.2 T-cell receptor segments in exposed individuals relative to the comparison group (p = 0.03 and p = 0.003, respectively). It was also shown that the distribution of the Vβ-segments of the T-cell receptor was uneven in both study groups. However, there was no significant difference between the repertoires of the T-cell receptor of the studied groups by the Gini-TCR index (p = 0.14).","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"522 1","pages":"404 - 409"},"PeriodicalIF":0.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0