利氏乳杆菌II型核苷脱氧核糖基转移酶催化的酶转糖基化反应制备6-叠氮-2-氯嘌呤-2′-脱氧核糖苷

IF 0.7 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2025-09-21 DOI:10.1134/S160767292560040X

C S Alexeev, M A Konkina, N N Kurochkin, M S Drenichev

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引用次数: 0

摘要

6-叠氮-2-氯嘌呤-2'-脱氧核苷是制备取代杂环基6位的改性2-氯嘌呤核苷的重要前体，通过酶转糖基化得到。6-叠氮-2-氯嘌呤-2'-脱氧核苷也可用作光交联剂来研究核酸与蛋白质的相互作用。采用莱希曼乳杆菌ⅱ型核苷脱氧核糖基转移酶作为生物催化剂。以7-甲基-2'-脱氧鸟苷为碳水化合物残基供体，确定了生成6-叠氮-2-氯嘌呤-2'-脱氧核苷的最佳条件。

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Preparation of 6-Azido-2-Chloropurine-2'-Deoxyriboside by Enzymatic Transglycosylation Reaction Catalyzed by Lactobacillus leichmannii Type II Nucleoside Deoxyribosyltransferase.

6-azido-2-chloropurine-2'-deoxyriboside, a valuable precursor for the preparation of modified 2-chloropurine nucleosides substituted at the 6-position of the heterocyclic base, was obtained by enzymatic transglycosylation. 6-azido-2-chloropurine-2'-deoxyriboside can also be used as a photocross-linking agent to study the nucleic acids-proteins interactions. A type II nucleoside deoxyribosyltransferase from Lactobacillus leichmannii was used as a biocatalyst. The optimal conditions for the formation of 6-azido-2-chloropurine-2'-deoxyriboside using 7-methyl-2'-deoxyguanosine as a carbohydrate residue donor were determined.

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.