Doklady Biochemistry and Biophysics最新文献

{"title":"Determinants of DNA Binding by Plant Homologs of 3-Methyladenine-DNA Glycosylase.","authors":"D V Petrova, L M Kulishova, A V Endutkin, M M Amanova, E P Panferova, B Yu, C Ma, H Li, I R Grin, D O Zharkov","doi":"10.1134/S1607672925601854","DOIUrl":"https://doi.org/10.1134/S1607672925601854","url":null,"abstract":"<p><p>Tag proteins, widespread among prokaryotes, are DNA glycosylases that participate in the repair of alkylated DNA excising 3-methyladenine. Among eukaryotes, Tag homologs are found only in plants, but they have not yet been characterized. Here, we report the first study of a plant Tag homolog, BvTAG1 from sugar beet (Beta vulgaris). Unlike bacterial Tags, BvTAG1 lacks activity towards alkylated DNA, which can be explained by the mutation of the critical catalytic Glu residue to Ser. At the same time, BvTAG1 exhibits a high affinity for undamaged DNA, mediated by a long N-terminal tail characteristic of all plant Tag homologs. This binding further increases in the presence of free 3-methyladenine. Our results suggest that plant Tag homologs may be required for regulating the plant response to genotoxic stress, rather than for DNA repair.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of RNA Tertiary Structure on Trans-Splicing of the mod(mdg4) Gene in Drosophila melanogaster.","authors":"Iu V Soldatova, O Beginyazova, P G Georgiev, M V Tikhonov","doi":"10.1134/S1607672925601696","DOIUrl":"https://doi.org/10.1134/S1607672925601696","url":null,"abstract":"<p><p>Trans-splicing is a rare variant of the splicing process, wherein exons are joined between different pre-mRNAs. This mechanism enables the formation of a broad spectrum of mRNAs encoded by a single locus. The most prominent example in Drosophila melanogaster is the mod(mdg4) locus, where trans-splicing results in the production of more than 30 mRNAs encoding protein isoforms with distinct C-terminal domains. Previous studies have demonstrated that sequences in the 5'-region of the fourth intron of the mod(mdg4) locus play a pivotal role in initiating trans-splicing. In the present study, we show that the integration of MS2 repeats, which form a stable secondary RNA structure, into the sequence of the fourth intron completely suppressed trans-splicing. Conversely, the insertion of MS2 repeats immediately upstream of the intron only marginally reduced the efficiency of this process. Modifications of the sequences of the fourth intron that induce the formation of an additional stem loop in the pre-mRNA also negatively impacted trans-splicing. These findings underscore the significant role of RNA secondary structures in regulating trans-splicing.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the Role of Transvection in the Regulation of Initiators That Determine Activity of the Regulatory Domains of the Drosophila melanogaster Abd-B Gene.","authors":"V O Dubrovskaya, E N Kozlov, P G Georgiev, O V Kyrchanova","doi":"10.1134/S1607672925601726","DOIUrl":"https://doi.org/10.1134/S1607672925601726","url":null,"abstract":"<p><p>The iab-5 domain regulates transcription of the Abdominal-B (Abd-B) gene in A5 and A6 segments throughout Drosophila development. The iab-5 domain contains an initiator, which determines domain activity, and two enhancers that activate Abd-B in the A5 and A6 cuticle cells, which ensures pigmentation of these segments in adult males. In the present study, we demonstrated that deletion of the iab-5 initiator on one chromosome can negatively affect the initiator located on the homologous chromosome. This is reflected in the appearance of unpigmented spots on segment A5 in males. Isolation of the domain by an insulator and deletion of the boundary sequences that recruit Polycomb proteins enhance the effect of transvection, which inactivates initiator activity in trans. Thus, the interaction between initiators located on homologous chromosomes determines the formation of a stable activation signal for the regulatory domain throughout the development of Drosophila.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trans-Splicing Enhances Processivity of RNA Polymerase II and Suppresses Polyadenylation Signals.","authors":"Iu V Soldatova, O Beginyazova, L S Melnikova, A K Golovnin, O G Maksimenko, P G Georgiev, M V Tikhonov","doi":"10.1134/S1607672925601738","DOIUrl":"https://doi.org/10.1134/S1607672925601738","url":null,"abstract":"<p><p>Trans-splicing, the process where exons from two different pre-mRNA molecules are joined, is an efficient mechanism for generating diverse isoforms encoded by a single locus. This process has been most thoroughly studied in the mod(mdg4) locus of Drosophila. This locus comprises one gene encoding the constant N-terminal part of the Mod(mdg4) protein and five genes that encode over thirty 3'-exons clustered together, determining the diversity of C-terminal domains. In this study, transgenic lines were obtained where a strong polyadenylation signal from the SV40 virus was inserted into two 3'-exon clusters with the aim of halting transcription and, consequently, reducing the frequency of trans-splicing between the constant exons of the mod(mdg4) gene and the 3'-exons of the modified clusters. Unexpectedly, it was found that SV40 polyadenylation signals had no effect on either the transcription level of the clusters or the efficiency of trans-splicing. The results indicate the ability of RNA polymerase II, transcribing the 3'-exons of the mod(mdg4) locus, to overcome even strong polyadenylation signals from SV40.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Effectiveness of Long-Term Therapy with Russian Rituximab Biosimilar (Acellbia) in Sjogren's Disease in Real Clinical Practice.","authors":"B D Chaltsev, V I Vasilyev, A V Torgashina, Yu I Khvan, E B Rodionova, E I Selifanova, T N Safonova, L A Semenova, M V Burtseva, S I Glukhova","doi":"10.1134/S1607672925700255","DOIUrl":"https://doi.org/10.1134/S1607672925700255","url":null,"abstract":"&lt;p&gt;&lt;p&gt;to evaluate the effectiveness of long-term therapy with Russian rituximab (RTX) biosimilar in Sjögren's disease (SjD) in real-life clinical practice. MATERIALS AND METHODS: -The retrospective study included 53 patients with SjD (Russian 2001 criteria and ACR/EULAR (American College of Rheumatology/European Alliance of Associations for Rheumatology) 2016 criteria), observed at the Nasonova Research Institute of Rheumatology from 2017 to 2024 and receiving long-term RTX therapy (Russian biosimilar Acellbia®, BIOCAD). The signs of clinical and laboratory activity of the disease, stomatological and ophthalmological tests, as well as the incidence of new systemic manifestations and lymphomas were assessed dynamically. RESULTS: -The median duration of RTX therapy was 27 [19; 55] months, and the median total dose was 4 [3.5; 5.5] g. Before the therapy, 13 (25%) patients had recurrent parotitis, which was relieved in all patients during the therapy. Persistent enlargement of the salivary glands was observed in 10 (20.4%) patients, in 9 of them it was relieved. A significant increase in stimulated saliva flow was found (from 1.5 [0.5; 3] to 2.4 [1.4; 3.5] mL; p = 0.002), an increase in salivation was found in 51% of patients, stabilization in 28.6%, and deterioration in 20.4%. When assessing the ultrasound dynamics of the salivary glands, the size of hypoechoic avascular lesions significantly decreased (from 1.8 [1.3; 2.3] to 1.3 [1.1; 1.5] mm; p&lt;0.001), and according to the ultrasound activity index, stabilization was noted in 67.4% of patients, improvement in 27.9%, and deterioration in 4.7% of patients. When assessing the dynamics of sialography, the size of cavities significantly decreased (from 1.5 [1.5; 2.5] to 1.0 [0; 1.5] mm; p &lt; 0.001), and according to the assessment of sialographic stages, stabilization was noted in 67.5% of patients, improvement in 32.5% of patients, and deterioration was not noted in any patient. When assessing the lacrimal glands function, a significant increase in lacrimation was found according to the stimulated Schirmer's test (from 6 [3.75; 12] to 8 [5; 15] mm; p = 0.005); an increase in lacrimation was noted in 38% of patients, stabilization in 40.6%, and a decrease in 21.4%. When assessing the tear break-up time, a tendency towards its increase was noted, but statistically insignificant (from 5 [3.75; 9.25] to 5.5 [4; 9] sec; p = 0.35). Corneal epitheliopathy during the therapy was relieved in 44% and persisted in 56% of patients; worsening of corneal epitheliopathy during the treatment was observed in a few patients, while no cases of ulcer formation or perforation of the cornea were recorded. During the therapy, a significant decrease in the levels of erythrocyte sedimentation rate, gamma globulins, IgG, IgA, IgM, rheumatoid factor, an increase in the C3 complement level, and the elimination of monoclonal gammopathy were observed, while the dynamics of the C4 complement level and cryoglobulinemia were multidire","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Late Boundary Complex (LBC) Selectively Binds the Fab-7/Fab-8 Boundaries, but not Fab-6 or Mcp, in the Abd-B Regulatory Region of Drosophila melanogaster.","authors":"A N Ibragimov, O V Kyrchanova, P G Georgiev, P Schedl","doi":"10.1134/S160767292560174X","DOIUrl":"https://doi.org/10.1134/S160767292560174X","url":null,"abstract":"<p><p>Boundary elements in the Drosophila Bithorax complex partition the Abd-B regulatory landscape into autonomous domains that require insulation and boundary bypass. We surveyed Large Boundary Complex (LBC) occupancy across Abd-B boundaries and detected binding at Fab-7 and Fab-8, but not at Fab-6 or at Mcp (the boundary between Abd-B and abd-A). Despite lacking LBC binding, Fab-6 supports boundary bypass, demonstrating that bypass can occur without LBC. Fab-7 and Fab-8 share two conserved motifs (\"green\" and \"blue\"); however, mutational analysis shows that neither motif is required for LBC recruitment. These findings reveal selective, context-dependent LBC occupancy at Abd-B boundaries and imply that additional factor(s) and/or higher-order sequence organization underlie bypass activity. Thus, LBC is not universally required for boundary bypass, and its recruitment to Fab-7/8 is independent of the shared \"green/blue\" motifs.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blockers of Acetylcholine Receptors Affect the Hypotensive Effect of the Central Loop Fragment of the WTX Toxin.","authors":"M S Severyukhina, A M Ismailova, E R Shaykhutdinova, N S Egorova, I A Dyachenko, V I Tsetlin, Yu N Utkin","doi":"10.1134/S1607672925601684","DOIUrl":"https://doi.org/10.1134/S1607672925601684","url":null,"abstract":"<p><p>We previously demonstrated that fragments of the central loop of the non-conventional toxin WTX reduce blood pressure in rats and inhibit certain nicotinic acetylcholine receptor subtypes [10]. In the present study, we examined the effects of hexamethonium and methylicaconitine, which are nicotinic acetylcholine receptor inhibitors, as well as of atropine, a muscarinic acetylcholine receptor inhibitor, on the hypotensive effect of the WTX central loop fragment. For this purpose, these compounds were administered intravenously before the peptide. We found that hexamethonium and methylicaconitine enhanced the fragment's hypotensive effect, while atropine weakened this effect. Only methylicaconitine enhanced the tachycardic effect of the peptide. These data indicate the involvement of both nicotinic and muscarinic acetylcholine receptors in the hemodynamic effects of the WTX central loop fragment.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of Rotenone Cytotoxicity in the Presence of Bach1 Inhibitors.","authors":"S V Nikulin, D Olkhovik, A V Razumovskaya, M O Silkina, A A Zakhariants, K V Klycheva, G A Khairetdinova, I G Gazaryan, D M Hushpulian","doi":"10.1134/S1607672925601817","DOIUrl":"https://doi.org/10.1134/S1607672925601817","url":null,"abstract":"<p><p>A novel trend in anticancer therapy is based on the combination of cytotoxic and metabolic drugs. Bach1 is a transcription factor activating glycolysis and increasing proliferation and metastatic potential in cancer cells. The cytotoxic effect was evaluated for a combination of rotenone, an inhibitor of mitochondrial respiration, and two different inhibitors of Bach1 transcription factor in the prostate cancer cell lines (PC3 and Du145) and colorectal cancer (HT-29 and HCT-116) in a kinetic mode. An enhancement of the cytotoxic action of the combination therapy was observed only for the prostate cancer cell lines PC3 and Du145. No enhancement of cytotoxicity of zinc porphyrin in the presence of rotenone was observed for the НСТ-116 line. The HT-29 line was not sensitive to either inhibitor or their combination with rotenone at 24 h incubation. According to the PCR results, HT-29 was the only line showing an extremely high activation of heme oxygenase 1 (HMOX1) in the presence of the Bach1 inhibitor, pointing to the highest level of the antioxidant defense in this cell line. The sensitivity of the prostate cancer cell lines to the combination therapy points to the significant differences in the metabolism between the prostate and colorectal cell lines.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of D/H Isotope Exchange in Stabilizing Trinucleotide Repeat Expansions in the CAG Tract of the ATXN2 Gene.","authors":"A A Dorohova, I M Chernukha, L V Fedulova, J L Hernandez-Caceres, M I Drobotenko, S S Dzhimak","doi":"10.1134/S1607672925601659","DOIUrl":"https://doi.org/10.1134/S1607672925601659","url":null,"abstract":"<p><p>CAG repeats in the first exon of the ATXN2 gene play a key role in the development of a number of neurodegenerative diseases. Understanding the mechanisms underlying CAG tract expansions and the factors influencing them could lead to the development of new methods for the prevention and treatment of these diseases. It was established that a single D/H substitution of a hydrogen bond in the CAG tract increases its stability, thereby reducing the likelihood of formation of secondary structures that interfere with the reading of genetic information from the glutamine-coding region. CAA interruptions in the CAG tract in specific locations can have a similar, but weaker, stabilizing effect.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Algorithm for Axial Involvement in Psoriatic Arthritis.","authors":"E E Gubar, T V Korotaeva, L D Vorobyova, E Yu Loginova, Yu L Korsakova, P O Tremaskina, A V Smirnov, M M Urumova, S I Glukhova","doi":"10.1134/S1607672925700243","DOIUrl":"https://doi.org/10.1134/S1607672925700243","url":null,"abstract":"<p><p>To develop a unified diagnostic algorithm for axial psoriatic arthritis (axPsA). MATERIALS AND METHODS. : A total of 122 patients with psoriatic arthritis (PsA), duration less than 10 years, were included in the study according to CASPAR criteria, provided that they also had axial involvement. Axial involvement was detected in case of radiographic sacroiliitis [(rSI); bilateral grade ≥2 or unilateral grade ≥ 3] or active MRI SI (MRI-SI), or ≥ 1 syndesmophyte(s) of the cervical and/or lumbar spine (CS/LS), or facet joints ankyloses of the CS. Patients were evaluated for the presence of inflammatory back pain (IBP) by ASAS criteria. Back pain lasting over 3 months, that did not meet ASAS criteria was considered chronic back pain (chrBP). HLA-B27 antigen status was observed. Results and discussion. IBP was identified in 87 (71.3%), chrBP-in 35 (28.7%) patients, 49 (40.2%) patients had older age (>40 years) at back pain onset; 120 (98.4%) patients had peripheral arthritis, 75 (61.5%)-dactylitis, 69 (56.6%)-enthesitis, 122 (100%)-psoriasis, 90 (73.8%)-nail psoriasis. Isolated axial disease without peripheral arthritis was found in 2 (1.6%) patients. rSI was detected in 85 (69.7%) patients, in 28 of 85 (32.9%) patients rSI developed without IBP. Spinal lesions of the LS and CS were found in 100 (82.0%) patients, chunky \"non-marginal\" syndesmophytes-in 60 (49.2%), asymmetrical syndesmophytes of the LS-in 22 of 72 (30.6%), paravertebral ossification-in 5 (4.1%) patients. Isolated spinal lesions without rSI were found in 37 (30.3%), isolated spinal lesions without rSI or MRI-SI-in 21 (17.2%) patients. HLA-B27 was observed in 27 of 86 (31.4%) examined patients. Diagnostic algorithm for axPsA was developed. All PsA patients, regardless whether they experienced IBP/chrBP or not, must undergo diagnostic imaging: pelvis, LS and CS X-ray. In patients without rSI, MRI of the sacroiliac joints should be performed. AxPsA diagnosis must be confirmed by imaging. Axial involvement is detected in case of rSI or MRI-SI, or ≥1 syndesmophyte(s) of the CS/LS, or facet joints ankyloses of the CS.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}