Acta Physiologiae Plantarum最新文献

{"title":"Thriving on half: low nutritional demand in Stephanopodium engleri involves adjustments in photosynthetic apparatus functionality","authors":"Evandro Alves Vieira,&nbsp;Luiz Palhares Neto,&nbsp;Joana Patrícia Pantoja Serrão Filgueira,&nbsp;Marina Ludmila Conor Salles,&nbsp;Rafael Valadares,&nbsp;Silvio J. Ramos,&nbsp;Markus Gastauer,&nbsp;Cecílio Frois Caldeira","doi":"10.1007/s11738-025-03844-y","DOIUrl":"10.1007/s11738-025-03844-y","url":null,"abstract":"<div><p>Cultivating threatened plant species is vital for conservation, safeguarding against extinction by ensuring propagation and maintaining genetic diversity. Here, we conducted an integrative morphological, physiological, and quantitative proteomic analysis of <i>Stephanopodium engleri</i> plants grown under different substrates and nutritional sources. <i>Stephanopodium engleri</i> is a threatened tree endemic to the Iron Quadrangle, a Brazilian mining region. Different nutritional sources resulted in changes in the growth of <i>S. engleri</i>, with N, P, and micronutrients identified as the most limiting nutrients. The species exhibited maximum growth with half of the applied fertilization, suggesting a low nutritional requirement. The highest growth was related to adjustments in the photosynthetic apparatus and protein regulation, with no significant differences in morpho-anatomical traits. The most representative proteins displayed a diverse array of metabolic functions, particularly related to iron and zinc homeostasis, photosynthesis and energy pathways and reactive oxygen species signaling. Plants with lower carbon assimilation and growth exhibited down-regulation of these proteins. Our findings provide valuable insights into <i>S</i>. <i>engleri</i> physiology and adaptations, emphasizing the importance of minimum fertilization to produce high-quality seedlings. These results also support broader conservation efforts aimed at preventing the species from becoming extinct, including reinforcing natural populations, establishing new ones, and supporting the maintenance of germplasm banks and living collections.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 11","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-025-03844-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145315792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Targeted profiling reveals metabolic perturbations in cryptogein-cotransformed hairy root cultures of Nicotiana tabacum","authors":"Mahesh Kumar,&nbsp;Sumita Jha,&nbsp;Adinpunya Mitra","doi":"10.1007/s11738-025-03845-x","DOIUrl":"10.1007/s11738-025-03845-x","url":null,"abstract":"","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 11","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145284463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of dihydroquercetin reduces oxidative damage and enhances drought resistance in wheat","authors":"Qiyu Wang,&nbsp;Jiaqing Guo,&nbsp;Chengjie Xu,&nbsp;Wensi Tang,&nbsp;Kai Chen,&nbsp;Yulong Wang,&nbsp;Yongbin Zhou,&nbsp;Jun Chen,&nbsp;Zhaoshi Xu,&nbsp;Shuguang Wang,&nbsp;Youzhi Ma,&nbsp;Ming Chen,&nbsp;Daizhen Sun","doi":"10.1007/s11738-025-03836-y","DOIUrl":"10.1007/s11738-025-03836-y","url":null,"abstract":"<div><p>Drought stress is one of the main abiotic stressors affecting wheat yield. In recent years, plant-derived compounds have played a key role in improving wheat stress resistance and have been widely used to enhance crop drought resistance and yield. Currently, flavonoids, as important secondary metabolites in plants, are related to drought resistance. In this study, we found through screening that the flavonoid compound dihydroquercetin can improve the drought resistance of wheat. The results indicate that exogenous dihydroquercetin can significantly improve the survival rate, relative water content, fresh weight, and dry weight of wheat seedlings under drought conditions. Biochemical assays combined with transcriptome analysis demonstrate that under drought stress, dihydroquercetin simultaneously enhances antioxidant capacity and upregulates the expression of <i>β-glucosidase </i>(<i>BGLU2</i>) and <i>aldehyde dehydrogenase </i>(<i>ALDH</i>) in the phenylpropanoid biosynthesis pathway. These changes collectively enhance the antioxidant capacity of wheat and reduce the content of superoxide anions and hydrogen peroxide. Dihydroquercetin also upregulated the expression of important genes in the glycerophospholipid metabolism pathway, including <i>phospholipase D </i>(<i>PLD</i>), <i>no special phospholipase C </i>(<i>NPC</i>), and <i>glycerophosphodiesterase </i>(<i>GDPD</i>), alleviating damage to the cell membrane, reducing malondialdehyde content. Therefore, the application of dihydroquercetin improves drought resistance in wheat by promoting antioxidant capacity and alleviating drought-induced oxidative damage. This study lays the groundwork for implementing dihydroquercetin to boost wheat's drought resilience in agricultural settings.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 11","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145284462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging trends in the application of pulsed magnetic fields for modulating plant growth and development: mechanisms, dynamics, and potential impact","authors":"Mukhtar Iderawumi Abdulraheem,&nbsp;Abiodun Yusuff Moshood,&nbsp;Papita H. Gourkhede,&nbsp;Linze Li,&nbsp;Yanyan Zhang,&nbsp;Gholaremza Abdi,&nbsp;Vijaya Raghavan,&nbsp;Jiandong Hu","doi":"10.1007/s11738-025-03843-z","DOIUrl":"10.1007/s11738-025-03843-z","url":null,"abstract":"<div><p>The increasing global food demand necessitates innovative and sustainable methods to enhance plant growth and productivity without relying excessively on chemical inputs. Pulsed magnetic fields (PMFs) have emerged as a non-chemical alternative with the potential to modulate key physiological and biochemical processes in plants. However, the precise mechanisms through which PMFs influence plant growth and development, as well as their varying effects under standard and stress conditions, remain areas of active investigation. This review aims to consolidate recent findings on the application of PMFs in modulating plant growth and development, with a particular focus on elucidating the underlying mechanisms, the dynamic interactions, and the potential agricultural applications. Emphasis is placed on the bio-electromagnetic interactions underlying PMF-induced changes in plant metabolism, stress resilience, and crop productivity. By understanding these interactions, researchers can optimize PMF parameters for targeted agricultural applications, balancing benefits and potential drawbacks. While challenges persist in terms of scalability and result variability, advancement in bio-electromagnetic research offers promising opportunities for integrating PMFs into sustainable agricultural practices. Ultimately, PMFs represent a frontier technology with significant implications for improving crop resilience, resource efficiency, and food security in an environmentally responsible manner.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 10","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145242720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic analysis of cyclin-dependent kinase (CDK) genes and the role of PbCDK9, PbCDK17, and PbCDK18 in cell proliferation in pear fruit","authors":"Si-Qi Zheng,&nbsp;Jia-Ning Kang,&nbsp;Pei-Zhuo Liu,&nbsp;Jia-Ying Ou-Yang,&nbsp;Tao Zhou,&nbsp;Zheng-Mao Zhang,&nbsp;Zhi-Hua Guo,&nbsp;Xue-Ping Wang,&nbsp;Shao-Ling Zhang,&nbsp;Chao Gu","doi":"10.1007/s11738-025-03842-0","DOIUrl":"10.1007/s11738-025-03842-0","url":null,"abstract":"<div><p>Cyclin-dependent kinases (CDKs) critical regulators of cell proliferation; however, their role in fruit size remains largely unexplored. This study identified 35 <i>CDK</i> genes from the pear genome, which were categorized into eight groups. The expression levels of 15 <i>CDK</i> genes exhibited a correlation with cell number variations during pear fruit development. Combining with the gene expression levels, <i>PbCDK9</i>, <i>PbCDK17</i>, and <i>PbCDK18</i> were selected for over-expression analysis in pear fruit callus. Transgenic fruit calli displayed a greater number of cells compared to control calli, suggesting the involvement of <i>PbCDK9</i>, <i>PbCDK17</i>, and <i>PbCDK18</i> in cell proliferation. Moreover, four concentrations of exogenous forchlorfenuron (CPPU), a synthetic cytokinin, were applied to fruitlets on the tree, resulting in a significant increase in fruit size at 1 and 25 mg/L CPPU. Notably, the expression levels of <i>PbCDK17</i> and <i>PbCDK18</i> were increased in CPPU-treated fruits compared to untreated fruits. These results suggest that exogenous CPPU treatment promotes cell proliferation by enhancing the expression of <i>PbCDK17</i> and <i>PbCDK18</i>, leading to increased fruit size. The findings provide a foundational understanding of the regulatory mechanisms underlying fruit size in agricultural production.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 10","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145242721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic Colletotrichum causes apoptosis-mediated cell death in Spodoptera frugiperda cells: implications for biocontrol and pest management","authors":"Kodsara Ramachandra Kiran,&nbsp;Vishwanath Bhat Deepika,&nbsp;Arya Kaniyassery,&nbsp;Keshava Prasad,&nbsp;Shama Prasada Kabekkodu,&nbsp;Thokur Sreepathy Murali,&nbsp;Annamalai Muthusamy","doi":"10.1007/s11738-025-03841-1","DOIUrl":"10.1007/s11738-025-03841-1","url":null,"abstract":"<div><p>Endophytic fungi symbiotically reside within plant tissues without eliciting apparent pathological responses. Positive interactions between endophytic fungi and plants help the host overcome biotic and abiotic stressors. <i>Spodoptera frugiperda</i> is a fall armyworm that devastates the revenue of farmers worldwide and is a major threat to food security. Here, we investigated the cytotoxicity of extracts of endophytic fungi isolated from brinjal (<i>Solanum melongena</i> L.) var. Mattu Gulla on the insect cell line Sf21 (<i>Spodoptera frugiperda</i> ovarian cells). Among the 13 endophytic fungi tested, <i>Colletotrichum</i> sp. presented the greatest cytotoxic ability, with an IC₅₀ of 103.6 µg mL⁻¹. LC‒MS analysis revealed the presence of several fungal metabolites (232), and 12 metabolites were identified, among which regiolone is known to have potent insecticidal activity. The fungal extract disrupted oxidative homeostasis in Sf21 cells by increasing the total reactive oxygen species (ROS) content according to the treatment concentration and duration. The mitochondrial membrane potential analysis by flow cytometry indicated the presence of depolarized mitochondria in cells treated with the fungal extract. Furthermore, fragmented mitochondria and clear signs of apoptosis, including nuclear condensation, the presence of necklace, ring-like structures and collapsed nuclei, were observed. Our data revealed that the <i>Colletotrichum</i> sp. extract had cytotoxic effects by inducing apoptosis in vitro, which could be explored with respect to the <i>Colletotrichum</i> sp. extract in further investigations.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 10","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-025-03841-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145242736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring key genes of the alternative respiration pathway, uncoupling, and calcium-binding proteins in methanol-treated rapeseed under drought stress","authors":"Narges Yazarloo,&nbsp;Mohammad Mahdi Taghvaei,&nbsp;Mohammad Mohsenzadeh Golfazani,&nbsp;Habibollah Samizadeh Lahiji","doi":"10.1007/s11738-025-03838-w","DOIUrl":"10.1007/s11738-025-03838-w","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Key message&lt;/h3&gt;&lt;p&gt;The research aimed at studying the impact of drought stress on rapeseed and evaluated the role of methanol foliar spraying in providing a source of carbon dioxide during its deficiency conditions. Further, the focus was on the key genes responsible for the alternative respiration pathway, uncoupling, and calcium-binding proteins.&lt;/p&gt;&lt;h3&gt;Abstract&lt;/h3&gt;&lt;p&gt;Oilseeds are the largest food reserves in the world after cereal. One of the most significant sources of edible oil is rapeseed. However, environmental stressors, such as drought, play a crucial role in reducing crop yield worldwide. This study was conducted to investigate the impact of drought stress on genes and to analyze the protein-protein interactions among key genes related to alternative respiration, uncoupling, and calcium-binding proteins. Additionally, the research aimed to detect any miRNAs that could potentially modulate target genes in the drought-sensitive (Hyola308) and drought-tolerant (SLM046) rapeseed genotypes. Gene network analysis using STRING v11.5 and cytoHubba (MMC method) identified &lt;i&gt;UCP1, NDB2, NDA2&lt;/i&gt;, and &lt;i&gt;AOX1A&lt;/i&gt; as major hub genes. To explore their post-transcriptional regulation, we used psRNATarget (expectation threshold ≤ 5) along with Brassicaceae-specific miRNAs from miRBase v22. This analysis predicted 22 microRNAs targeting these four rapeseed genes, primarily from the miR169, miR168, miR319, miR390, miR397, miR393, and miR171 families. These miRNAs are predicted to regulate mitochondrial alternative respiration pathways under drought stress, primarily by modulating key genes involved in ROS scavenging and cellular energy homeostasis. Among the identified miRNAs, bna-miR169a/b/n, bna-miR168a/b, bna-miR403, bna-miR390a/b/c, bna-miR397a/b, bna-miR393, and bna-miR171f/g were found to be rapeseed-specific. For instance, bna-miR169a/b/n, known to regulate &lt;i&gt;NF-YA&lt;/i&gt; transcription factors during drought stress, targets &lt;i&gt;NDB2&lt;/i&gt;, a gene critical for maintaining NADH oxidation. bna-miR393 is involved in modulating auxin signaling, while bna-miR171f/g targets &lt;i&gt;SCL6&lt;/i&gt; transcripts, influencing genes related to root architecture and contributing to stress adaptation. Furthermore, miR403 is known to modulate responses to both drought and cold stress, and miR390 targets &lt;i&gt;ARF&lt;/i&gt; genes, which influence root development and play a crucial role in stress tolerance mechanisms. qRT‐PCR (three biological × three technical replicates) on drought‐stress (DR; 30% field capacity) and methanol‐treated (DM; 20% v/v foliar spray) plants (CL = fully irrigated control) showed that methanol application significantly upregulated &lt;i&gt;UCP1&lt;/i&gt; in tolerant plants at 24 h post‐stress and induced &lt;i&gt;NDA2/AOX1A&lt;/i&gt; in sensitive plants at 72 h (DR) and 24 h (DM). Our results demonstrate that foliar methanol enhances drought resilience by boosting alternative respiration and suggest that the identified miRNAs may serve as post‐transcriptional regulators o","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 10","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145242722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild barley (Hordeum vulgare L. subsp. Spontaneum (C. Koch) Thell.): a noxious weed species in Iran and an untapped genetic resource for barley cultivar development- molecular approach","authors":"Mojtaba Hosseini,&nbsp;Mehdi Mojab,&nbsp;Mohsen Yassaie,&nbsp;Eskandar Zand,&nbsp;Eshagh Keshtkar,&nbsp;Samuel Revolinski,&nbsp;Acer VanWallendael,&nbsp;Ebrahim Mamnoie","doi":"10.1007/s11738-025-03839-9","DOIUrl":"10.1007/s11738-025-03839-9","url":null,"abstract":"<div><p>Wild barley is a weed species descended from a shared ancestor of cultivated barley (<i>Hordeum vulgare</i>). Originating in the Fertile Crescent of the Middle East. <i>H. vulgare</i> L. subsp. <i>Spontaneum</i> (C. Koch) Thell is now widely distributed in the western region of Iran, along the Zagros mountain range. However, on one hand, wild barley has become a problematic, difficult-to-control noxious weed in Iran's wheat fields over the past two decades, causing significant damage to the country's cereal production. On the other hand, the phenotypic and genetic diversity demonstrated among Iranian wild barley populations makes them a valuable genetic resource for barley improvement. This review article discusses strategies for controlling this weedy plant and examines the ecology, phenotypic plasticity for adaptation to harsh environments, and genetic diversity in wild barley populations, all of which make wild barley an invaluable genetic resource. Temperature and rainfall play important roles in natural selection pressures in populations of wild barley, driving the maintenance of genetic variation between environments. Wild barley could serve as a model plant species for studying the relationship between genomic diversity, physiological adaptability to terrestrial habitats, and geographical divergence in grass species. The high level of phenotypic variation in traits, such as disease resistance, environmental stress tolerance, and herbicide resistance in Iranian wild barley indicates an untapped, rich, and extensive source of genetic variation for improving traits in <i>H. vulgare</i> cultivars.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 10","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-025-03839-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145242737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous jasmonic acid increases Barringtonia racemosa tolerance to heavily lead-contaminated soil","authors":"Yutong Lin,&nbsp;Xixian Liang,&nbsp;Qiuwei Huang,&nbsp;Haiqing Jiang,&nbsp;Caizhen Luo,&nbsp;Wenjia Chen,&nbsp;Yuting Yang,&nbsp;Xiaohui Tan,&nbsp;Fang Liang","doi":"10.1007/s11738-025-03837-x","DOIUrl":"10.1007/s11738-025-03837-x","url":null,"abstract":"<div><p>Highly toxic lead (Pb) is essentially a threat to the ecological security of mangrove wetlands in the South China Sea. <i>Barringtonia racemosa</i> is a typical and endangered semi-mangrove, which may be suffering from heavy Pb pollution. Jasmonic acid (JA) can regulate plant defense mechanisms under Pb stress. This study explored the defense and adaptive mechanisms of <i>B. racemosa</i> under the regulation of JA through controlled experiments with a Pb concentration gradient. Results showed that exogenous JA significantly increased the palisade tissue and root cortex thickness of <i>B. racemosa</i> under different Pb concentrations, while the thickness of the vascular bundle diameter had the opposite response. Peroxidase activity, proline and chlorophyll concentrations were significantly increased under Pb stress. It can be concluded that increasing palisade tissue thickness, concentrations of photosynthetic pigments and antioxidant enzymes are aided by JA and constitute a vital Pb-stress response strategy for <i>B. racemosa</i>. JA enhanced antioxidant defense mechanisms, mitigating Pb toxicity and reducing reactive oxygen species produced under heavy Pb stress. Remarkably, under high Pb concentration, the bioconcentration factor and Pb absorption of <i>B. racemosa</i> were significantly reduced, improving its tolerance to Pb stress. This study revealed the regulatory mechanism of the exogenous hormone JA on the tolerance of <i>B. racemosa</i> under Pb stress, which has a scientific guiding significance for the protection of germplasm resources of <i>B. racemosa</i> in the heavily Pb-polluted areas of mangrove wetlands.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 9","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NtOGG1 regulates tobacco seed germination involving ethylene and reactive oxygen species pathways under salt stress","authors":"Yongzhi Niu,&nbsp;Wenlong Suo,&nbsp;Guoping Wang,&nbsp;Chengjing Wang,&nbsp;Dandan Wang,&nbsp;Zepeng Wu,&nbsp;Zhoufei Wang,&nbsp;Yunye Zheng","doi":"10.1007/s11738-025-03835-z","DOIUrl":"10.1007/s11738-025-03835-z","url":null,"abstract":"<div><p>Salt stress significantly inhibits seed germination in tobacco, yet the molecular mechanisms underlying this process remain largely unexplored. In this study, we demonstrated that <i>NtOGG1</i>, encoding an 8-oxoguanine DNA glycosylase, functions as a positive regulator of salt tolerance during seed germination. Under 150 mM NaCl stress, the overexpressing <i>NtOGG1</i> line (<i>NtOGG1</i>-OE) exhibited higher germination and seedling percentage compared to wildtype (WT), whereas CRISPR–Cas9 knockout mutant showed reductions in both parameters. RNA-Seq analysis revealed that differentially expressed genes (DEGs) in the <i>NtOGG1</i>-OE line, including ethylene-responsive transcription factors (ERFs), respiratory burst oxidase homologs (RBOHs), and catalase (CAT) genes, are implicated in the mitogen-activated protein kinase (MAPK) signaling pathway. Further qRT-PCR and physiological assays confirmed that the enhanced ethylene responses and reduced accumulation of reactive oxygen species (ROS) significantly promote seed germination in the <i>NtOGG1</i>-OE line under salt stress. These findings establish <i>NtOGG1</i> as a key regulator influencing seed germination under salt stress, providing a promising molecular target for breeding salt-tolerant tobacco cultivars.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 9","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}