Acta Physiologiae Plantarum最新文献

Metabolic adjustment via microbial agents and biochar synergy enhances mercury stress tolerance in Vigna radiata L. 通过微生物制剂和生物炭协同作用的代谢调节增强了辐射维纳对汞胁迫的耐受性。

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-05-05 DOI: 10.1007/s11738-025-03806-4

Shirwan Malaie, Latifeh Pourakbar, Sina Siavash Moghaddam, Nabi Khezrinejad, Jianbo Xiao

{"title":"Metabolic adjustment via microbial agents and biochar synergy enhances mercury stress tolerance in Vigna radiata L.","authors":"Shirwan Malaie, Latifeh Pourakbar, Sina Siavash Moghaddam, Nabi Khezrinejad, Jianbo Xiao","doi":"10.1007/s11738-025-03806-4","DOIUrl":"10.1007/s11738-025-03806-4","url":null,"abstract":"<div><p>Mercury (Hg) as an abiotic stressor poses significant challenges to plant growth. This study investigates the response of <i>Vigna radiata</i> L plant to three levels of Hg stress (0, 20, and 40 mg/L) using a hydroponic system, inoculated with microbial biostimulators. The two-factorial experiment focused on plant growth, total soluble sugars (TSS), and free amino acids. Results indicated a decrease in plant biomass with increasing stress severity. Under moderate stress (20 mg/L Hg), arbuscular mycorrhiza (AM) was effective in preserving biomass, while under severe stress (40 mg/L Hg), a combination of biochar, AM, and bacterial biostimulants (BAB) was most effective. The highest biomass was recorded by combined treatment of bacteria and AM (BaAM) although it dropped sharply under stress conditions. Except for BaAM, TSS content increased in all moderate stress treatments, particularly with AM, and was notably enhanced by BAB under severe stress. <i>V. radiata</i> plants generally showed an increase in Proline, Phenylalanine, Tyrosine, Tryptophan, Asparagine, Glycine, and Valin levels when exposed to Hg stress, whereas Aspartate decreased across all stress treatments. Higher Hg tolerance was linked to greater accumulation of TSS and specific free amino acids like Proline, Phenylalanine, and Asparagine. The interaction with microbial inoculation suggests that <i>V. radiata</i> plants adapt to Hg toxicity by altering their sugar and amino acid profiles, which serve as multifunctional molecules and precursors for stress resistance metabolites.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotechnology in viticulture: alleviating lime stress in 1103 Paulsen Rootstock with iron oxide nanoparticles (Fe3O4-NPs) 纳米技术在葡萄栽培中的应用：利用氧化铁纳米颗粒（Fe3O4-NPs）缓解1103 Paulsen砧木的石灰胁迫

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-04-30 DOI: 10.1007/s11738-025-03805-5

Selda Daler, Ozkan Kaya, Duran Kaplan

{"title":"Nanotechnology in viticulture: alleviating lime stress in 1103 Paulsen Rootstock with iron oxide nanoparticles (Fe3O4-NPs)","authors":"Selda Daler, Ozkan Kaya, Duran Kaplan","doi":"10.1007/s11738-025-03805-5","DOIUrl":"10.1007/s11738-025-03805-5","url":null,"abstract":"<div><p>High lime content in agricultural soils poses a significant challenge to crop production, particularly in viticulture. Due to the persistent and detrimental effects of lime stress on plant growth, the present study investigated the potential of iron oxide nanoparticles (Fe<sub>3</sub>O<sub>4</sub>-NPs) to mitigate lime-induced stress in 1103 Paulsen American grapevine rootstock. We examined the effects of Fe₃O₄-NPs (0, 0.01, 0.1, and 1 ppm) under varying lime stress conditions (0%, 20%, 40%, and 60% CaCO<sub>3</sub>). Our findings revealed that increasing lime content progressively inhibited grapevine growth, with significant reductions in shoot fresh weight, root fresh weight, shoot length, and leaf number. Fe<sub>3</sub>O<sub>4</sub>-NP application demonstrated pronounced protective effects: 0.1 ppm Fe<sub>3</sub>O<sub>4</sub>-NPs optimized growth under non-stressed conditions, while 1 ppm Fe<sub>3</sub>O<sub>4</sub>-NPs significantly improved plant performance under 60% lime stress. Notably, nanoparticle treatments mitigated oxidative stress by reducing membrane damage, lipid peroxidation, and leaf temperature while maintaining photosynthetic efficiency and osmotic balance. Fe<sub>3</sub>O<sub>4</sub>-NPs demonstrated significant potential in mitigating lime-induced stress in grapevines, with optimal concentrations of 0.1 ppm for low–moderate lime environments and 1 ppm for high lime content areas. These findings provide a targeted nanobiotechnological approach to enhance grapevine resilience in calcareous soils, advancing sustainable viticulture strategies.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-025-03805-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manipulation of glycine–serine and flavanone metabolism to maintain plasma membrane stability and improve drought tolerance of millet 控制谷子甘氨酸-丝氨酸和黄酮代谢维持质膜稳定性和提高耐旱性

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-04-28 DOI: 10.1007/s11738-025-03807-3

Mengjiao Wang, Bianyin Wang, Yajie Liu, Xiao Zhang, Jixun Guo, Tao Zhang, Wenying Zhang, Lianxuan Shi

{"title":"Manipulation of glycine–serine and flavanone metabolism to maintain plasma membrane stability and improve drought tolerance of millet","authors":"Mengjiao Wang, Bianyin Wang, Yajie Liu, Xiao Zhang, Jixun Guo, Tao Zhang, Wenying Zhang, Lianxuan Shi","doi":"10.1007/s11738-025-03807-3","DOIUrl":"10.1007/s11738-025-03807-3","url":null,"abstract":"<div><p>Drought has become a serious threat to food security and sustainable development. Studying the mechanism of drought tolerance of plants is crucial to develop drought-tolerant crops, which helps to address food security. This study aimed to explore the metabolic pathways associated with drought tolerance in foxtail millet (<i>Setaria italica</i> L.). The roots of drought-tolerant millet variety Zhonggu2 and the common variety An04 were selected for a comparative analysis of the changes in growth, ions, differentially expressed genes and metabolites between the two accessions in response to drought by transcriptomics and metabolomics. Under drought conditions, the root length of both varieties increased significantly (‘Zhonggu2’ > ‘An04’). The contents of K<sup>+</sup>, Ca<sup>2+</sup> and NO<sub>3</sub><sup>−</sup> in Zhonggu2 increased significantly. In phospholipid metabolism, the phospholipase D genes were down-regulated and the contents of inositol and ethanolamine were increased in Zhonggu2. The relative contents of glycine and serine were increased, and the gene encoding serine hydroxymethyltransferase in glycine–serine metabolism were upregulated. In flavanone metabolism, the contents of tyrosine, coumaric acid, naringenin-7-<i>O</i>-glucoside, ferulic acid and vanillic acid were increased and the expression levels of genes encoding 4-coumarate-CoA ligase and shikimate-<i>O</i>-hydroxycinnamoyltransferase were upregulated in the Zhonggu2. Integrated analyses showed that Zhonggu2 prevented the accumulation of reactive oxygen species by promoting glycine–serine metabolism and flavanone metabolism, thereby improving drought resistance. At the same time, Zhonggu2 may also delay the phospholipid decomposition, thereby maintaining the stability of the plasma membrane under drought stress. This study provides important insights for breeding, laying the foundation for future crop genetic improvement.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abiotic stress-inducible purple acid phosphatase from Solanum lycopersicum is targeted to peroxisome via non-canonical peroxisome targeting signal type 1 非生物胁迫诱导型紫色酸性磷酸酶通过非典型过氧化物酶体靶向信号型1靶向于过氧化物酶体

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-04-22 DOI: 10.1007/s11738-025-03800-w

Sabiha Tarafdar, Gopal Chowdhary

{"title":"Abiotic stress-inducible purple acid phosphatase from Solanum lycopersicum is targeted to peroxisome via non-canonical peroxisome targeting signal type 1","authors":"Sabiha Tarafdar, Gopal Chowdhary","doi":"10.1007/s11738-025-03800-w","DOIUrl":"10.1007/s11738-025-03800-w","url":null,"abstract":"<div><p>Nutrient limitations combined with abiotic stress conditions, pose some of the most challenging scenarios regarding agricultural productivity. Phosphorus is an important macronutrient for plants. Though present in ample quantity in soil, the available phosphorous to plant is severely limited. Purple acid phosphatase (PAP) is a special class of acid phosphatase that plays a significant role in these phosphorous starvation situations. Primarily PAPs are secretory, however, a few intracellular PAPs have also been reported, which are primarily localized in various subcellular compartments. Here, we have identified a PAP from tomato, named <i>Solanum lycopersicum</i> PAP (SlPAP) 19, which was demonstrated to be localized in peroxisome and was also found to be induced by multiple abiotic stress conditions. It showed the highest upregulation by low-temperature stress followed by high-temperature and osmotic stress. The detailed in silico analysis revealed it to retain the traditional N-terminal transmembrane domain and the glycosylation domain, suggesting that the endoplasmic reticulum mediated peroxisome targeting pathway of PTS1 proteins could well be a novel peroxisomal import pathway. Intracellular PAPs have previously been demonstrated to possess additional peroxidase activity, which could be involved in reactive oxygen species (ROS) homeostasis under abiotic stress conditions. Being involved in supplying phosphorous macronutrient, upregulated by multiple abiotic stress conditions and intracellular localization, SlPAP19 could serve as a significant candidate for abiotic stress tolerance in plants.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morpho-physiological attributes, antioxidant activities, proximate seed analysis, and phytoremediative prospective of two castor bean genotypes in lead spiked soil 含铅土壤中两种蓖麻基因型的形态-生理属性、抗氧化活性、种子近似分析和植物补救前景

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-04-22 DOI: 10.1007/s11738-025-03801-9

Sahar Naveed, Sajid Mahmood, Wajid Ishaque, Muhammad Akhtar

{"title":"Morpho-physiological attributes, antioxidant activities, proximate seed analysis, and phytoremediative prospective of two castor bean genotypes in lead spiked soil","authors":"Sahar Naveed, Sajid Mahmood, Wajid Ishaque, Muhammad Akhtar","doi":"10.1007/s11738-025-03801-9","DOIUrl":"10.1007/s11738-025-03801-9","url":null,"abstract":"<div><p>Anthropogenic activities like mining, smelting, and the use of batteries and paints discharge heavy metals, particularly lead (Pb), into the soil. Castor bean (<i>Ricinus communis</i> L.), a non-edible oil seed crop, demonstrated a considerable aptitude for phytoremediation of heavy metal contaminated soils. Furthermore, environmentally sustainable attributes and economic benefits of castor bean are attracting the attention of researchers. Hence, the current study evaluated the phytoremediation capabilities of two castor bean genotypes (NIAB-2020 and DS-30) on Pb-contaminated soil (400 and 800 mg/kg) until physiological maturity in a pot study under greenhouse conditions. Results indicated that plant agronomic traits, including plant height, biomass, root length, leaf number, and leaf area, declined under Pb stress. Physiological parameters, such as relative water content, stomatal conductance, transpiration rate, and photosynthetic activity, also decreased, while electrolyte leakage increased. Total activity of antioxidants like superoxide dismutase (SOD) and peroxidase (POD) were declined; however, catalase (CAT) and ascorbate peroxidase (APX) increased in both castor genotypes. Additionally, osmolytes content like total soluble proteins (TSP) and total free amino acids (TFA) were decreased, while total soluble sugars (TSS) and total phenolic content (TPC) increased under Pb stress. Metal uptake response in the roots and shoots exhibited a significant capacity for Pb accumulation, demonstrating effectiveness of castor bean in reducing Pb concentration. Proximate seed analyses showed a slight reduction in seed quality at 400 and 800 mg/kg, while Pb concentration remained undetected. Consequently, this study underscores the capability of castor bean as a viable entrant for restoring the soils contaminated with Pb.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brassinosteroids alleviate of harmful effects of prolonged water deficit in young Euterpe oleracea palms increasing water use efficiency 油菜素内酯减轻了长时间水分亏缺对幼树油葵的有害影响，提高了水分利用效率

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-04-17 DOI: 10.1007/s11738-025-03804-6

Florine Alves de Sousa Pinheiro, Sebastião de Oliveira Maia Júnior, Jailma Ribeiro de Andrade, Patrick Costa Silva, Danielle Lopes Aguiar, Lorena Silva Campos, Maria Rita da Silva Andrade Leonel, Niedja Bezerra Costa, Mara de Menezes de Assis Gomes, Fábio Afonso Mazzei Moura de Assis Figueiredo, Fabrício de Oliveira Reis, Paulo Henrique Aragão Catunda, Tiago Massi Ferraz

{"title":"Brassinosteroids alleviate of harmful effects of prolonged water deficit in young Euterpe oleracea palms increasing water use efficiency","authors":"Florine Alves de Sousa Pinheiro, Sebastião de Oliveira Maia Júnior, Jailma Ribeiro de Andrade, Patrick Costa Silva, Danielle Lopes Aguiar, Lorena Silva Campos, Maria Rita da Silva Andrade Leonel, Niedja Bezerra Costa, Mara de Menezes de Assis Gomes, Fábio Afonso Mazzei Moura de Assis Figueiredo, Fabrício de Oliveira Reis, Paulo Henrique Aragão Catunda, Tiago Massi Ferraz","doi":"10.1007/s11738-025-03804-6","DOIUrl":"10.1007/s11738-025-03804-6","url":null,"abstract":"<div><p>Water deficit has a negative effect on the physiological aspects of plants, such as stomatal closure and consequent decline in photosynthetic carbon assimilation. Numerous water deficit mitigation strategies have been investigated, such as the use of bioregulators to minimize the damage caused. This study aimed at assessing the effects of brassinosteroids on the physiological aspects of açaí seedlings in inducing drought tolerance. The experiment was conducted using two water conditions (well-watered and water-deficit plants) and three brassinosteroid concentrations (0, 0.05 and 0.10 µM of 24-epibrassinolide—EBL), with six repetitions. At 120 days, seedlings were transplanted to pots and watered, leaving the soil near field capacity for 56 days. Next, a group of plants were well-watered, and another submitted to water deficit for 18 days. Water deficit reduced gas exchange and photosynthetic efficiency with a lower decrease at EBL concentrations of 0.05 and 0.10 µM, while larger declines were observed in plants without EBL. Relative water content and leaf succulence were maintained in water-deficit plants, while proline content rose, mainly with 0.10 µM of EBL. Applying EBL also improved water use efficiency and maintained the leaf chlorophyll and stem dry matter of stressed plants. It was concluded that leaf brassinosteroid application alleviate of harmful effects of water deficit in young açaí plants, promoting proline accumulation, which increases water use efficiency, and maintaining photosynthetic pigments and water status, contributing to improving drought tolerance in açaí.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipoxygenase 2 is a potential resistance determinant in Brassica juncea – B. fruticulosa derived lines against Lipaphis erysimi infestation 脂氧合酶2是甘蓝型芸苔系对褐斑脂肪酶侵染的潜在抗性决定因子

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-04-16 DOI: 10.1007/s11738-025-03803-7

Jatin Aggarwal, Rimaljeet Kaur, Amandeep Mittal, Chhaya Atri, Mehak Gupta, Anju Sharma

引用次数: 0

Temperature-dependent conformational changes in Arabidopsis DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2A 拟南芥脱水响应元件结合蛋白2A的温度依赖性构象变化

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-04-11 DOI: 10.1007/s11738-025-03799-0

Petri Tähtinen, Hiroaki Fujii

{"title":"Temperature-dependent conformational changes in Arabidopsis DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2A","authors":"Petri Tähtinen, Hiroaki Fujii","doi":"10.1007/s11738-025-03799-0","DOIUrl":"10.1007/s11738-025-03799-0","url":null,"abstract":"<div><p>With the recent rise in global temperatures, understanding plant heat stress responses has become an urgent challenge. The DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2A (DREB2A) is one of the key transcription factors involved in plant responses to heat stress. Previous studies show that DREB2A degrades at 23 °C, while it accumulates at 37 °C in <i>Arabidopsis</i>, leading to heat-induced gene expression. However, the direct impact of temperature on DREB2A protein itself remains insufficiently understood. This study investigates the effect of temperature on the DREB2A protein by expressing recombinant DREB2A in <i>Escherichia coli</i>. Results demonstrate that DREB2A accumulates in <i>E. coli</i> at 37 °C but not at 23 °C, a pattern also observed in <i>Arabidopsis</i>, despite the differences between these organisms. Circular dichroism (CD) spectroscopy further revealed structural alterations in DREB2A between 23 °C and 37 °C, though specific details remain unclear. Taken together, these findings suggest that temperature-induced conformational changes occur in DREB2A between 23 °C and 37 °C, which may play a role in regulating its stability. This knowledge also indicates that 37 °C-induced stability is a contributing factor to successful purification of full-length recombinant DREB2A protein.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11738-025-03799-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyto-genotoxic assay of dimethoate-30 through chromosomal events in Allium cepa L. roots 通过薤白根中的染色体事件对二甲氧基苯甲酸-30 进行细胞遗传毒性分析

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-04-08 DOI: 10.1007/s11738-025-03798-1

Kiran Gupta, Amit Kumar, Khushwant Singh

{"title":"Cyto-genotoxic assay of dimethoate-30 through chromosomal events in Allium cepa L. roots","authors":"Kiran Gupta, Amit Kumar, Khushwant Singh","doi":"10.1007/s11738-025-03798-1","DOIUrl":"10.1007/s11738-025-03798-1","url":null,"abstract":"<div><p>A potentially genotoxic insecticide that is widely used in agriculture is dimethoate-30. This study focused on assessing the cyto-genotoxic impact of dimethoate-30 applying the <i>Allium cepa</i> bioassay, to understand its impact on biota. Roots were treated with 20, 40, 60, 80, and 100 mgl<sup>−1</sup> dimethoate-30 concentrations for 24, 48, and 96 h, and root growth was measured using mitotic indices. The result showed a significant dose-dependent retardation in mitotic index over to control value, indicating cytotoxicity. Various cytological abnormalities were also observed, including c-metaphase, anaphase bridges, chromosome fragmentation, sticky metaphase and anaphase, and laggard chromosome. In addition, the frequency of micronuclei, aberrant cells percentage, and relative abnormality rate was increased, indicating genotoxicity. These findings indicate that dimethoate-30 may have cytotoxic and genotoxic effects, suggesting that it could be harmful and potentially carcinogenic. Therefore, it is not recommended to use higher concentrations of dimethoate-30 due to its cytogenotoxicity and potential to kill plants.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative profiling of metabolites in Validamycin A-treated black gram (Vigna mungo L. Hepper) under drought stress by gas chromatography: a way forward to mitigate drought stress 用气相色谱法比较干旱胁迫下缬霉素a处理的黑克兰（Vigna mungo L. Hepper）代谢物：缓解干旱胁迫的一种方法

IF 2.4 4区生物学

Acta Physiologiae Plantarum Pub Date : 2025-04-08 DOI: 10.1007/s11738-025-03795-4

Mini Madhavan Lysal, Jasmine Juliet Richard, Renganathan Vellaichamy Gandhimeyyan, Vellaikumar Sampathrajan, Renuka Raman

{"title":"Comparative profiling of metabolites in Validamycin A-treated black gram (Vigna mungo L. Hepper) under drought stress by gas chromatography: a way forward to mitigate drought stress","authors":"Mini Madhavan Lysal, Jasmine Juliet Richard, Renganathan Vellaichamy Gandhimeyyan, Vellaikumar Sampathrajan, Renuka Raman","doi":"10.1007/s11738-025-03795-4","DOIUrl":"10.1007/s11738-025-03795-4","url":null,"abstract":"<div><p>Validamycin A (VMA), an inhibitor of trehalase was applied as a foliar spray in black gram (<i>Vigna mungo</i> L. Hepper) plants imposed to drought stress to intervene the trehalose pathway, which is involved in conferring desiccation tolerance. Untargeted metabolomics study was conducted using GC–MS, with data analyzed by the Metaboloanalyst platform. Enzyme assays confirmed the decreased activity of trehalase and increased accumulation of trehalose to 1.8 fold in drought-stressed VMA treated leaf tissues when compared to that of control (watered). These samples also revealed significantly high starch content (14.29 mgg<sup>−1</sup>) compared to that of drought stress (9.36 mgg<sup>−1</sup>). In drought-stressed plants, amylase and acid invertase activities of leaf increased significantly and metabolomic profiling showed enhanced accumulation of glucose (33-fold) and fructose (sevenfold) which are the products of these enzymes. The heat map of drought-stressed VMA treatment revealed a unique accumulation of palmitic acid, 8,10-dioxaheptadecane, 9-octadecenoic acid, stearic acid, N-acetyl glutamic acid, 3-alpha mannobiose, glyceryl glycoside, pentanedioic acid, myristic acid, sorbitol, 1,4-butanediol, arabitol, tagatose and sucrose. Based on Variable Importance in Projection (VIP) score plot, mannose, lyxose, and oxalic acid are selected as marker metabolites linked with drought tolerance due to VMA treatment. Pathway impact analysis revealed that galactose metabolic pathway was the most affected. This study provides a comprehensive understanding of the metabolites associated with trehalose accumulation that can provide strategies to mitigate drought stress in black gram.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0