{"title":"盐胁迫下NtOGG1通过乙烯和活性氧途径调控烟草种子萌发","authors":"Yongzhi Niu, Wenlong Suo, Guoping Wang, Chengjing Wang, Dandan Wang, Zepeng Wu, Zhoufei Wang, Yunye Zheng","doi":"10.1007/s11738-025-03835-z","DOIUrl":null,"url":null,"abstract":"<div><p>Salt stress significantly inhibits seed germination in tobacco, yet the molecular mechanisms underlying this process remain largely unexplored. In this study, we demonstrated that <i>NtOGG1</i>, encoding an 8-oxoguanine DNA glycosylase, functions as a positive regulator of salt tolerance during seed germination. Under 150 mM NaCl stress, the overexpressing <i>NtOGG1</i> line (<i>NtOGG1</i>-OE) exhibited higher germination and seedling percentage compared to wildtype (WT), whereas CRISPR–Cas9 knockout mutant showed reductions in both parameters. RNA-Seq analysis revealed that differentially expressed genes (DEGs) in the <i>NtOGG1</i>-OE line, including ethylene-responsive transcription factors (ERFs), respiratory burst oxidase homologs (RBOHs), and catalase (CAT) genes, are implicated in the mitogen-activated protein kinase (MAPK) signaling pathway. Further qRT-PCR and physiological assays confirmed that the enhanced ethylene responses and reduced accumulation of reactive oxygen species (ROS) significantly promote seed germination in the <i>NtOGG1</i>-OE line under salt stress. These findings establish <i>NtOGG1</i> as a key regulator influencing seed germination under salt stress, providing a promising molecular target for breeding salt-tolerant tobacco cultivars.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"47 9","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"NtOGG1 regulates tobacco seed germination involving ethylene and reactive oxygen species pathways under salt stress\",\"authors\":\"Yongzhi Niu, Wenlong Suo, Guoping Wang, Chengjing Wang, Dandan Wang, Zepeng Wu, Zhoufei Wang, Yunye Zheng\",\"doi\":\"10.1007/s11738-025-03835-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Salt stress significantly inhibits seed germination in tobacco, yet the molecular mechanisms underlying this process remain largely unexplored. In this study, we demonstrated that <i>NtOGG1</i>, encoding an 8-oxoguanine DNA glycosylase, functions as a positive regulator of salt tolerance during seed germination. Under 150 mM NaCl stress, the overexpressing <i>NtOGG1</i> line (<i>NtOGG1</i>-OE) exhibited higher germination and seedling percentage compared to wildtype (WT), whereas CRISPR–Cas9 knockout mutant showed reductions in both parameters. RNA-Seq analysis revealed that differentially expressed genes (DEGs) in the <i>NtOGG1</i>-OE line, including ethylene-responsive transcription factors (ERFs), respiratory burst oxidase homologs (RBOHs), and catalase (CAT) genes, are implicated in the mitogen-activated protein kinase (MAPK) signaling pathway. Further qRT-PCR and physiological assays confirmed that the enhanced ethylene responses and reduced accumulation of reactive oxygen species (ROS) significantly promote seed germination in the <i>NtOGG1</i>-OE line under salt stress. These findings establish <i>NtOGG1</i> as a key regulator influencing seed germination under salt stress, providing a promising molecular target for breeding salt-tolerant tobacco cultivars.</p></div>\",\"PeriodicalId\":6973,\"journal\":{\"name\":\"Acta Physiologiae Plantarum\",\"volume\":\"47 9\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Physiologiae Plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11738-025-03835-z\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Physiologiae Plantarum","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s11738-025-03835-z","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
盐胁迫显著抑制烟草种子萌发,但这一过程背后的分子机制仍未被充分探索。在这项研究中,我们证明了编码8-氧鸟嘌呤DNA糖基化酶的NtOGG1在种子萌发过程中作为耐盐性的正调节因子。在150 mM NaCl胁迫下,与野生型(WT)相比,过表达的NtOGG1株系(NtOGG1- oe)的发芽率和成苗率均较高,而CRISPR-Cas9敲除突变体在这两个参数上均有所降低。RNA-Seq分析显示,NtOGG1-OE系的差异表达基因(DEGs),包括乙烯应答转录因子(ERFs)、呼吸爆发氧化酶同源物(RBOHs)和过氧化氢酶(CAT)基因,与丝裂原活化蛋白激酶(MAPK)信号通路有关。进一步的qRT-PCR和生理实验证实,盐胁迫下乙烯响应的增强和活性氧(ROS)积累的减少显著促进了NtOGG1-OE种子的萌发。这些发现表明NtOGG1是盐胁迫下影响烟草种子萌发的关键调控因子,为耐盐烟草品种的选育提供了一个有希望的分子靶点。
NtOGG1 regulates tobacco seed germination involving ethylene and reactive oxygen species pathways under salt stress
Salt stress significantly inhibits seed germination in tobacco, yet the molecular mechanisms underlying this process remain largely unexplored. In this study, we demonstrated that NtOGG1, encoding an 8-oxoguanine DNA glycosylase, functions as a positive regulator of salt tolerance during seed germination. Under 150 mM NaCl stress, the overexpressing NtOGG1 line (NtOGG1-OE) exhibited higher germination and seedling percentage compared to wildtype (WT), whereas CRISPR–Cas9 knockout mutant showed reductions in both parameters. RNA-Seq analysis revealed that differentially expressed genes (DEGs) in the NtOGG1-OE line, including ethylene-responsive transcription factors (ERFs), respiratory burst oxidase homologs (RBOHs), and catalase (CAT) genes, are implicated in the mitogen-activated protein kinase (MAPK) signaling pathway. Further qRT-PCR and physiological assays confirmed that the enhanced ethylene responses and reduced accumulation of reactive oxygen species (ROS) significantly promote seed germination in the NtOGG1-OE line under salt stress. These findings establish NtOGG1 as a key regulator influencing seed germination under salt stress, providing a promising molecular target for breeding salt-tolerant tobacco cultivars.
期刊介绍:
Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry.
The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.