American journal of physiology. Cell physiology最新文献

Physical effects of 3D microenvironments on confined cell behaviors. 三维微环境对封闭细胞行为的物理影响

IF 5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00288.2024

Bao-Qiong Lan, Ya-Jun Wang, Sai-Xi Yu, Wei Liu, Yan-Jun Liu

引用次数: 0

CD8⁺ tissue-resident memory T cells are essential in bleomycin-induced pulmonary fibrosis. CD8+组织驻留记忆T细胞对博莱霉素诱导的肺纤维化至关重要

IF 5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00368.2024

Xiao Feng, Fan Yu, Xin-Liang He, Pei-Pei Cheng, Qian Niu, Li-Qin Zhao, Qian Li, Xiao-Lin Cui, Zi-Heng Jia, Shu-Yi Ye, Li-Mei Liang, Lin-Jie Song, Liang Xiong, Fei Xiang, Xiao-Rong Wang, Wan-Li Ma, Hong Ye

{"title":"CD8+ tissue-resident memory T cells are essential in bleomycin-induced pulmonary fibrosis.","authors":"Xiao Feng, Fan Yu, Xin-Liang He, Pei-Pei Cheng, Qian Niu, Li-Qin Zhao, Qian Li, Xiao-Lin Cui, Zi-Heng Jia, Shu-Yi Ye, Li-Mei Liang, Lin-Jie Song, Liang Xiong, Fei Xiang, Xiao-Rong Wang, Wan-Li Ma, Hong Ye","doi":"10.1152/ajpcell.00368.2024","DOIUrl":"https://doi.org/10.1152/ajpcell.00368.2024","url":null,"abstract":"Human tissue-resident memory T (TRM) cells play a crucial role in protecting the body from infections and cancers. Recent research observed increased numbers of TRM cells in the lung tissues of idiopathic pulmonary fibrosis patient. However, the functional consequences of TRM cells in pulmonary fibrosis remain unclear. Here, we found that the numbers of TRM cells, especially the CD8+ subset, were increased in the mouse lung with bleomycin-induced pulmonary fibrosis. Increasing or decreasing CD8+ TRM cells in mouse lungs accordingly altered the severity of fibrosis. In addition, adoptive transfer of CD8+ T cells containing a large number of CD8+ TRM cells from fibrotic lungs was sufficient to induce pulmonary fibrosis in control mice. Treatment with CCL18 to induced CD8+ TRM cell expansion and exacerbated fibrosis, while blocking CCR8 prevented CD8+ TRM recruitment and inhibited pulmonary fibrosis. In conclusion, CD8+ TRM cells are essential for bleomycin-induced pulmonary fibrosis, and targeting CCL18/CCR8/CD8+ TRM cells may be a potential therapeutic approach.","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pik3c3 Expression Profiling in the Mouse Kidney and Its Role in Proximal Tubule Cell Physiology 小鼠肾脏中 Pik3c3 的表达谱分析及其在近端肾小管细胞生理学中的作用

IF 5.5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00564.2023

Ting Liu, Jialing Yuan, Caihong Dai, Mystie X Chen, Jerry Fan, Benjamin D. Humphreys, David J. Fulton, Daniel T. Kleven, Xingjun Fan, Zheng Dong, Jian-Kang Chen

引用次数: 0

KLF3 impacts insulin sensitivity and glucose uptake in skeletal muscle KLF3 影响骨骼肌的胰岛素敏感性和葡萄糖摄取量

IF 5.5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00085.2024

Shuying Fu, Xiaocheng Gong, Keying Liang, Ke Ding, Li Qiu, Huice Cen, Hongli Du

引用次数: 0

Dual effects of targeting neuropilin-1 in lenvatinib-resistant hepatocellular carcinoma: inhibition of tumor growth and angiogenesis 在来伐替尼耐药肝细胞癌中靶向神经肽-1的双重作用：抑制肿瘤生长和血管生成

IF 5.5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00511.2024

Junjie Ao, Na Qiang, Hiroaki Kanzaki, Masato Nakamura, Risa Kakiuchi, Jiaqi Zhang, Ryuta Kojima, Keisuke Koroki, Masanori Inoue, Naoya Kanogawa, Ryo Nakagawa, Takayuki Kondo, Sadahisa Ogasawara, Shingo Nakamoto, Ryosuke Muroyama, Jun Kato, Naoya Kato

引用次数: 0

Melatonin mitigates matrix stiffness-induced disc degenetation by inhibiting reactive oxygen species and melatonin receptor mediated-PI3K/AKT/NF-κB pathway 褪黑激素通过抑制活性氧和褪黑激素受体介导的PI3K/AKT/NF-κB通路，减轻基质僵化诱发的椎间盘退化

IF 5.5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00630.2023

Zhihui Liang, Jia Song, Wen-ji Shangguan, Qiu-qi Zhang, Jiang Shao, Yue-hui Zhang

引用次数: 0

Bacterial lipopolysaccharide inhibits free thiamin uptake along the intestinal tract via interference with membrane expression of thiamin transporters-1 & -2. 细菌脂多糖通过干扰硫胺素转运体-1和-2的膜表达，抑制肠道对游离硫胺素的摄取。

IF 5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00570.2024

Selvaraj Anthonymuthu, Subrata Sabui, Kameron I Manzon, Alaullah Sheikh, James M Fleckenstein, Hamid M Said

{"title":"Bacterial lipopolysaccharide inhibits free thiamin uptake along the intestinal tract via interference with membrane expression of thiamin transporters-1 & -2.","authors":"Selvaraj Anthonymuthu, Subrata Sabui, Kameron I Manzon, Alaullah Sheikh, James M Fleckenstein, Hamid M Said","doi":"10.1152/ajpcell.00570.2024","DOIUrl":"https://doi.org/10.1152/ajpcell.00570.2024","url":null,"abstract":"This study examined the effect of exposure of small and large intestinal epithelial cells to the bacterial lipopolysaccharide (LPS) on uptake of free form of vitamin B1, i.e., thiamin. The intestinal tract encounters two sources of thiamin: diet and the gut microbiota. Absorption of thiamin in both the small and large intestine occurs via a carrier-mediated process that involves thiamin transporters-1 & -2 (THTR-1 & -2). Complementary in vitro (human duodenal epithelial HuTu-80 cells and human colonic epithelial NCM460 cells), in vivo (mice), and ex vivo (human primary differentiated enteroid and colonoid monolayers) models were used. The results showed that exposure to LPS causes a significant inhibition in carrier-mediated [3H]-thiamin uptake by small and large intestinal epithelia, with no change in levels of expression of THTR-1& -2 mRNAs and their total cellular proteins. However, a significant decrease in the fractions of the THTR-1& -2 proteins that are expressed at the cell membranes of these epithelial cells was observed. These effects of LPS appeared to involve a protein kinase A (PKA) signaling pathway as activating this pathway caused a reversal in the inhibition of thiamin uptake and level of expression of its transporters at the cell membrane. These findings demonstrate that exposure of gut epithelia to LPS (a situation that occurs under different pathological conditions) leads to inhibition in thiamin uptake due to a decrease in level of expression of its transporters at the cell membrane that is likely mediated via a PKA-signaling pathway.","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum for Hagen et al., volume 327, 2024, p. C372–C378 Hagen 等人的更正，第 327 卷，2024 年，第 C372-C378 页

IF 5.5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00326.2024_cor

引用次数: 0

PALMD haploinsufficiency aggravates extracellular matrix remodeling in vascular smooth muscle cells and promotes calcification. PALMD 单倍体缺陷会加剧血管平滑肌细胞细胞外基质的重塑并促进钙化。

IF 5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-09 DOI: 10.1152/ajpcell.00217.2024

Jichao Zhang, Zhao Yang, Congcong Zhang, Shijuan Gao, Yan Liu, Yingkai Li, Songyuan He, Jing Yao, Jie Du, Bin You, Yingchun Han

{"title":"PALMD haploinsufficiency aggravates extracellular matrix remodeling in vascular smooth muscle cells and promotes calcification.","authors":"Jichao Zhang, Zhao Yang, Congcong Zhang, Shijuan Gao, Yan Liu, Yingkai Li, Songyuan He, Jing Yao, Jie Du, Bin You, Yingchun Han","doi":"10.1152/ajpcell.00217.2024","DOIUrl":"https://doi.org/10.1152/ajpcell.00217.2024","url":null,"abstract":"Background: Reduced PALMD expression is strongly associated with the development of calcified aortic valve stenosis; however, the role of PALMD in vascular calcification remains unknown.Methods: Calcified arteries were collected from mice to detect PALMD expression. Heterozygous Palmd knockout (Palmd+/-) mice were established to explore the role of PALMD in subtotal nephrectomy-induced vascular calcification. RNA sequencing was applied to detect molecular changes in aortas from Palmd+/- mice. Primary Palmd+/- vascular smooth muscle cells (VSMCs) or PALMD silenced VSMCs by short interfering RNA (siRNA) were used to analyze PALMD function in phenotypic changes and calcification.Results: PALMD haploinsufficiency aggravated subtotal nephrectomy-induced vascular calcification. RNA sequencing analysis showed that loss of PALMD disturbed the synthesis and degradation of the extracellular matrix (ECM) in aortas, including collagens and matrix metalloproteinases (Col6a6, Mmp2, Mmp9, etc.). In vitro experiments revealed that PALMD deficient VSMCs were more susceptible to high phosphate induced calcification. Downregulation of SMAD6 expression and increased levels of p-SMAD2 were detected in Palmd+/- VSMCs, suggesting that TGF-β signaling may be involved in PALMD haploinsufficiency-induced vascular calcification.Conclusion: Our data revealed that PALMD haploinsufficiency causes ECM dysregulation in VSMCs and aggravates vascular calcification. Our findings suggest reduced PALMD expression is also linked to vascular calcification, and PALMD maybe a potential therapeutic target for this disease.","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of glucose-stimulated β-cell Nr4a1 expression impairs insulin secretion and glucose homeostasis. 葡萄糖刺激的β细胞Nr4a1表达缺失会损害胰岛素分泌和葡萄糖稳态。

IF 5 2区生物学

American journal of physiology. Cell physiology Pub Date : 2024-09-02 DOI: 10.1152/ajpcell.00315.2024

Jacob A Herring, Jacqueline E Crabtree, Jonathon T Hill, Jeffery S Tessem

{"title":"Loss of glucose-stimulated β-cell Nr4a1 expression impairs insulin secretion and glucose homeostasis.","authors":"Jacob A Herring, Jacqueline E Crabtree, Jonathon T Hill, Jeffery S Tessem","doi":"10.1152/ajpcell.00315.2024","DOIUrl":"https://doi.org/10.1152/ajpcell.00315.2024","url":null,"abstract":"A central aspect of type 2 diabetes is decreased functional β-cell mass. The orphan nuclear receptor Nr4a1 is critical for fuel utilization, but little is known regarding its regulation and function in the β-cell. Nr4a1 expression is decreased in type 2 diabetes rodent β-cells and type 2 diabetes patient islets. We have shown that Nr4a1 deficient mice have reduced β-cell mass and that Nr4a1 knock-down impairs glucose-stimulated insulin secretion (GSIS) in INS-1 832/13 β-cells. Here, we demonstrate that glucose concentration directly regulates β-cell Nr4a1 expression. We show that 11 mM glucose increases Nr4a1 expression in INS-1 832/13 β-cells and primary mouse islets. We show that glucose functions through the cAMP/PKA/CREB pathway to regulate Nr4a1 mRNA and protein expression. Using Nr4a1-/- animals, we show that Nr4a1 is necessary for GSIS and systemic glucose handling. Using RNA-seq, we define Nr4a1-regulated pathways in response to glucose in the mouse islet, including Glut2 expression. Our data suggests that Nr4a1 plays a critical role in the β-cells response to the fed state.","PeriodicalId":7585,"journal":{"name":"American journal of physiology. Cell physiology","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0