Acta Botanica Croatica最新文献

Micropropagationand optimisation of in vitro productionof the rare and threatened moss Entosthodonpulchellus (Funariaceae) 珍稀濒危苔藓 Entosthodonpulchellus（Funariaceae）的体外微繁殖和优化生产

IF 1.1 4区生物学

Acta Botanica Croatica Pub Date : 2024-07-23 DOI: 10.37427/botcro-2025-012

Marija V. Ćosić, Djordje P. Božović, M. Ignatov, E. Ignatova, Milorad M. Vujičić, A. Sabovljević, Marko S. Sabovljević

{"title":"Micropropagation\u0000and optimisation of in vitro production\u0000of the rare and threatened moss Entosthodon\u0000pulchellus (Funariaceae)","authors":"Marija V. Ćosić, Djordje P. Božović, M. Ignatov, E. Ignatova, Milorad M. Vujičić, A. Sabovljević, Marko S. Sabovljević","doi":"10.37427/botcro-2025-012","DOIUrl":"https://doi.org/10.37427/botcro-2025-012","url":null,"abstract":"The establishment of axenic cultures and the propagation of the rare moss Entosthodon pulchellus (H. Philip.) Brugués (Funariaceae), as well as the optimisation of its ex situ growth conditions and rapid biomass production were the goals of this study. The results obtained also provide insights into the developmental biology of this moss in in vitro conditions, particularly regarding basal media contents with or without supplements of selected plant growth regulators or sugars, i.e. its nutritional needs. The procedure for establishing axenic cultures of this rare and threatened moss species is described. The optimisation protocol of the growth and production conditions is elaborated. The spore germinability of dried herbarium samples remained rather high in the tested laboratory conditions. Suitability tests on different media types showed KNOP basal media to be the best fit for the rapid biomass production of both secondary protonemata and leafy gametophores, without the addition of sugars or plant growth regulators. However, sugar, namely sucrose, induced rapid and massive protonemal development and can be used when this developmental stage is needed.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141810987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cymbella stomachsis sp. nov.(Bacillariophyta; Cymbellaceae), a new species from Guangdong Province, China 中国广东省的一个新种--Cymbella stomachsis sp.

IF 1.1 4区生物学

Acta Botanica Croatica Pub Date : 2024-07-23 DOI: 10.37427/botcro-2025-010

Yi-Han Zhao, JI-SHU Guo, ZHENG-BIN Tang, Yun Zhang, Shao-Feng Huang, J. Kociolek, YAN-LING Li

{"title":"Cymbella stomachsis sp. nov.\u0000(Bacillariophyta; Cymbellaceae), a new species from Guangdong Province, China","authors":"Yi-Han Zhao, JI-SHU Guo, ZHENG-BIN Tang, Yun Zhang, Shao-Feng Huang, J. Kociolek, YAN-LING Li","doi":"10.37427/botcro-2025-010","DOIUrl":"https://doi.org/10.37427/botcro-2025-010","url":null,"abstract":"A new diatom species from the genus Cymbella C. Agardh is described from samples collected during a survey of freshwater diatoms from Modaomen Channel, Guangdong Province, China. With the aid of light microscopy (LM) and scanning electron microscopy (SEM), we give a detailed morphological description of Cymbella stomachsis sp. nov., which is placed in the genus Cymbella according to the shared features of asymmetry about the apical axis, deflection of the external terminal raphe fissures towards the dorsal margin, and the presence of apical pore fields and stigmata. Although it shares some similar structural features with C. tumida, C. stuxbergii, C. stuxbergioides, C. pesudostuxbergii, C. mexicana and C. australica, C. stomachsis sp. nov. is easily distinguished by the valve outline, the shape and density of areola, and the appearance of a lack of an intermissio at the central nodule. Especially, the structure of the intermissio is absent in similar taxa. This work is important for its enrichment of our understanding of the genus Cymbella and improvement of the database of diatom biodiversity and distribution in China.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141813029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The anatomy, micromorphology, and essential oils ofthe Turkish endemic and endangered species Alchemillaorduensis 土耳其特有的濒危物种 Alchemillaorduensis 的解剖学、微形态学和精油

IF 1.1 4区生物学

Acta Botanica Croatica Pub Date : 2024-07-23 DOI: 10.37427/botcro-2025-011

Öznur Ergen Akçin, Tuğba Özbucak, Şükran Öztürk, Hüseyin Ümit Uzunömeroğlu

{"title":"The anatomy, micromorphology, and essential oils of\u0000the Turkish endemic and endangered species Alchemilla\u0000orduensis","authors":"Öznur Ergen Akçin, Tuğba Özbucak, Şükran Öztürk, Hüseyin Ümit Uzunömeroğlu","doi":"10.37427/botcro-2025-011","DOIUrl":"https://doi.org/10.37427/botcro-2025-011","url":null,"abstract":"In this study, the anatomical and micromorphological characteristics of the vegetative organs and the essential oil constituents of the aerial and underground parts of the local and endangered endemic species A. orduensis Pawł. were evaluated. For anatomical study, sections of root, rhizome, stem, leaves and petiole were excised and stained with safranin/fast green mixture. Leaf and petiole structures were examined micromorphologically. Essential oil contents were determined by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME/GC-MS) analysis. The results showed that rectangular meristematic cells were present in the root. The leaf is of the bifacial and amphistomatic type. Stomata cells are of the anomocytic type. The stomatal index for the upper surface of the leaves is 0.04, while the stomatal index for the lower surface is 0.17. Druse crystals were found in the rhizome, stem and leaves. Among the various compounds identified, the most abundant groups in the aboveground parts are alcohols (39.81%) and ketones (14.99%) with 1-Octen-3-ol, 1-octan-3-one and borane- methyl sulfide complex as the main compounds. Terpenes (23.44%) and alcohols (11.82%), in which myrtenolis was the main compound, were most abundant in the underground parts.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141812714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid spread of the Mediterranean glycophyte Catapodium rigidum in Hungary 地中海糖生植物硬毛蕨（Catapodium rigidum）在匈牙利的快速传播

IF 1.1 4区生物学

Acta Botanica Croatica Pub Date : 2024-07-23 DOI: 10.37427/botcro-2025-002

Norbert Bauer, János Csiky, Attila Mesterházy, Mátyás Wolf, Dávid Schmidt

引用次数: 0

Carex distachya (Cyperaceae) with both subspecies in Europe 薹草，在欧洲有两个亚种

IF 1.1 4区生物学

Acta Botanica Croatica Pub Date : 2024-07-23 DOI: 10.37427/botcro-2025-013

Jacob Koopman, H. Więcław, Sandro Bogdanović, T. Denchev

引用次数: 0

Leaf phenotypic plasticity of European ash (Fraxinusexcelsior) at its northern range in Dinaric Alps 欧洲白蜡树（Fraxinusexcelsior）在迪纳拉阿尔卑斯山北部分布区的叶片表型可塑性

IF 1.1 4区生物学

Acta Botanica Croatica Pub Date : 2024-07-23 DOI: 10.37427/botcro-2025-001

Antonio Vidaković, Sandi Matijašević, Katarina Tumpa, Igor Poljak

{"title":"Leaf phenotypic plasticity of European ash (Fraxinus\u0000excelsior) at its northern range in Dinaric Alps","authors":"Antonio Vidaković, Sandi Matijašević, Katarina Tumpa, Igor Poljak","doi":"10.37427/botcro-2025-001","DOIUrl":"https://doi.org/10.37427/botcro-2025-001","url":null,"abstract":"The Dinaric Alps have been recognised on numerous occasions as a biodiversity hotspot. They host a variety of species with great importance in sustainable forestry operations and nature conservation. One such species is the European ash (Fraxinus excelsior L., Oleaceae), a broad-leaved, wind-pollinated and wind-dispersed forest tree. In this paper, we aimed to determine the morphological variability of the European ash populations of the northern Dinaric Alps. For this purpose, leaf samples from 10 individuals in seven populations were collected. Morphometric analysis of intra- and interpopulation variability was conducted using 19 morphological leaf traits. We determined great variability of trees within, and small variability among, populations. The variables that best discriminated studied populations were those relating to leaflet shape. Based on these variables, populations were grouped into two clusters. The first cluster encompassed individuals with acute leaflets, found in drier and rockier habitats, whereas the second cluster, defined by more rounded leaflets, was found in mesophilous and nutrient-rich habitats. However, this research revealed no influence of geographical or bioclimatic distances on morphological variability, which indicates that the rockiness and soil are most likely two predominant factors in shaping the phenotypic plasticity of European ash populations. These results are of great significance in the planning of future forest breeding programs, as populations from drier habitats are likely to persist and spread due to their adaptation to water scarcity, which will become more pronounced in the future.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141811250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seed morphological diversity of EgyptianAllium L. (Amaryllidaceae) and its taxonomic significance EgyptianAllium L.（Amaryllidaceae）种子形态多样性及其分类学意义

IF 1.1 4区生物学

Acta Botanica Croatica Pub Date : 2024-07-23 DOI: 10.37427/botcro-2025-014

Iman H. Nour, Ahmed K Osman, Rim S. Hamdy, Ibrahim A. El Garf

{"title":"Seed morphological diversity of Egyptian\u0000Allium L. (Amaryllidaceae) and its taxonomic significance","authors":"Iman H. Nour, Ahmed K Osman, Rim S. Hamdy, Ibrahim A. El Garf","doi":"10.37427/botcro-2025-014","DOIUrl":"https://doi.org/10.37427/botcro-2025-014","url":null,"abstract":"Allium L. (Amaryllidaceae, Allioideae, Allieae) has disputed generic delimitation and species boundaries, compounded by the proliferation of the species' synonyms. This study provides for the first time a comprehensive description of the seed morphology of native, endemic, and near-endemic species in Egypt and addresses the significance of seed traits for infrageneric classification. Twenty-two Allium taxa belonging to four subgenera and six sections were investigated using fresh or dry materials from their mature seeds. Thirty-eight quantitative and qualitative traits of the seeds' dorsal and ventral sides were investigated using stereomicroscopy and scanning electron microscopy (SEM). Statistical and multivariate analyses were performed. This work provides the first description of the seeds of 13 Allium taxa, including A. artemisietorum Eig & Feinbrun, A. barthianum Asch. & Schweinf., A. blomfieldianum Asch. & Schweinf., A. crameri Asch. & Boiss., A. desertorum Forssk., A. erdelii Zucc., A. mareoticum Bornm. & Gauba, A. papillare Boiss., A. roseum subsp. tourneuxii Boiss., A. sativum L., A. sinaiticum Boiss., A. spathaceum Steud. ex A.Rich., and A. trifoliatum Cirillo. This study reports for the first time a comparative investigation of dorsal seed surface traits against ventral traits, revealing conspicuous differences for most species and highlighting the most informative diagnostic seed traits for distinguishing taxa. Allium subg. Allium L. has a broader range of variation than any of the other subgenera.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141813310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Additional data on the ongoing naturalization of the non-nativewoody plant Duranta erecta ( Verbenaceae) in Sicily, Italy 关于意大利西西里岛非本地木本植物直立杜鹃（马鞭草科）正在归化的补充数据

IF 1.1 4区生物学

Acta Botanica Croatica Pub Date : 2024-07-23 DOI: 10.37427/botcro-2025-009

Salvatore Pasta, Pietro Lo Cascio, E. Badalamenti

引用次数: 0

Indicationsof programmed cell death in wheat roots upon exposure to silver nanoparticles 暴露于纳米银粒子后小麦根部细胞程序性死亡的迹象

IF 1.1 4区生物学

Acta Botanica Croatica Pub Date : 2024-07-23 DOI: 10.37427/botcro-2025-008

F. Yanık, F. Vardar

{"title":"Indications\u0000of programmed cell death in wheat roots upon exposure to silver nanoparticles","authors":"F. Yanık, F. Vardar","doi":"10.37427/botcro-2025-008","DOIUrl":"https://doi.org/10.37427/botcro-2025-008","url":null,"abstract":"Programmed cell death (PCD) can occur at every developmental stage as a plant’s response to various biotic and abiotic environmental factors. Silver nanoparticles (AgNPs) are widely used in consumer products and possess antimicrobial properties, making them important in assessing nanoparticle effects on plants. In the present study, we examined the impact of AgNPs (0, 0.5, 1, 5, 10, and 20 mg L-1) on wheat root PCD by evaluating parameters such as the mitotic index, chromosomal behaviors, nuclear deformation, cytochrome c release, caspase-1-like activity, and the expression of cysteine protease genes (TaVPE4, TaMCA1, and TaMCA4). Our findings revealed a dose-dependent decrease in the mitotic index ratio and increased chromosomal abnormalities induced by AgNPs. Additionally, we observed various hallmarks of PCD, including chromatin condensation, slight DNA smear, reduction in mitochondrial inner membrane potential, and cytochrome c release to the cytoplasm as well as increased caspase-1-like activity and TaVPE4 gene expression. Notably, the gene expressions of TaMCA1 and TaMCA4 were found to be antagonistically regulated by AgNPs, further indicating the induction of PCD by AgNP treatment. Overall, our study provides evidence of AgNP-induced PCD in wheat roots, elucidating the involvement of cysteine protease genes in this process.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141812315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overcoming seed dormancy and improving germination of Convolvulus persicus, an endangered coastal plant in north of Iran 克服伊朗北部一种濒危沿海植物 Convolvulus persicus 的种子休眠并提高其发芽率

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2024-06-13 DOI: 10.37427/botcro-2025-005

Razieh Bahadornejad Velashedi, Sedigheh Kelij, Naser Jafari

{"title":"Overcoming seed dormancy and improving germination of Convolvulus persicus, an endangered coastal plant in north of Iran","authors":"Razieh Bahadornejad Velashedi, Sedigheh Kelij, Naser Jafari","doi":"10.37427/botcro-2025-005","DOIUrl":"https://doi.org/10.37427/botcro-2025-005","url":null,"abstract":"Convolvulus persicus L. is an endemic endangered species distributed in the coastal regions of the Caspian Sea and the Black Sea that displays limited germination, potentially impacting its ability to regenerate. To gain an understanding of the dormancy status and germination needs of C. persicus, seed characteristics, seed coat permeability and different dormancy-breaking treatments were assessed. The results revealed that C. persicus seed coats are water-impermeable and that both cold and warm stratification were effective in breaking dormancy. Furthermore, GA3 pretreatment with combination of either cold or warm stratification proved successful in releasing dormancy. However, the highest germination percentage and rate as well as seed vigour was achieved by mechanical scarification followed by H2SO4 application and warm stratification. Warm stratification was recognized to be more favourable for overcoming seed dormancy and promoting seedling survival than cold stratification. Notably, the influence of population type on germination capacity was found to be negligible. These findings may facilitate the conservation and collection management of this threatened plant species, which is currently underrepresented in ex situ conservation efforts.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141347550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0