A. Shahzad, Mingzhou Qin, Mudassar Nazir, A. Shakoor, Motsim Billah, G. Zaib
{"title":"Response of Bacillus cereus on Zea mays under different doses of zinc sulphate","authors":"A. Shahzad, Mingzhou Qin, Mudassar Nazir, A. Shakoor, Motsim Billah, G. Zaib","doi":"10.37427/botcro-2021-022","DOIUrl":"https://doi.org/10.37427/botcro-2021-022","url":null,"abstract":"Anthropogenic activities have added a large amount of heavy metals to the environment. Heavy metal contaminants affect the physiological and biological properties of soil and plant health. Zinc (Zn) is an essential micronutrient and it promotes plant growth and development but\u0000a higher concentration of the metal causes reduction in plant growth. The present study was aimed to evaluate the response of Bacillus cereus on maize plants at different concentrations of ZnSO4 (20, 40 and 60 mg kg–1) amended in the soil under pot experiment conditions. The experiment was conducted by using complete randomized design (CRD) with three replications. Higher doses of ZnSO4 inhibited maize growth and nutrient uptake. However, inoculation of maize seeds with Bacillus cereus at 20 mg kg–1 concentration of ZnSO4 increased seed germination about 39% and plant height by 15%. Moreover, 17% increase in leaf length\u0000and a 7% increase in leaf number were observed as compared to control at 20 mg kg–1 concentrations of ZnSO4. Reductions in all growth parameters were observed with 60 mg kg–1 concentration of ZnSO4. The Zn uptake was 75% higher in treatment T8 (uninoculated seeds with 60 mg kg–1 concentration of ZnSO4) as compared to treatments which were inoculated and grown under different zinc concentrations. The results suggest that Bacillus cereus has good potential to remediate Zn from soil as well as to reduce the phyto-availibility and phytotoxicity of zinc.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49615122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Kaya, Sevil Yeniocak, İrem Aktay, O. Çelįk, Bilge Ozturk, Damla Ekin Ozkaya, Sevinc Guldag, Taner Mercan, S. Galatali, O. Akguller, Mehmet Ali Balci
{"title":"Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)","authors":"E. Kaya, Sevil Yeniocak, İrem Aktay, O. Çelįk, Bilge Ozturk, Damla Ekin Ozkaya, Sevinc Guldag, Taner Mercan, S. Galatali, O. Akguller, Mehmet Ali Balci","doi":"10.37427/botcro-2021-024","DOIUrl":"https://doi.org/10.37427/botcro-2021-024","url":null,"abstract":"Thymus cilicicus is an endemic Eastern Mediterranean element that has aromatic-medicinal properties. Its natural population spreads across gravelly ground and open rocky areas of South and Southwest Anatolia. The current study on in vitro propagation of T. cilicicus focused deeply\u0000on environmental applications such as the development of an optimum medium composition for efficient and genetically stable micropropagation\u0000and improved preservation procedures for long-time conservation of elite germplasms for further studies. For this purpose, MS and OM media were used individually and in combination with cytokinins, charcoal, AgNO3, Fe-EDDHA, and H3BO3. The raw data were statistically analyzed via\u0000the graph kernel method to optimize the nonlinear relationship between all parameters. The optimal proliferation medium for T. cilicicus was OM\u0000supplemented with a combination of 10 g L-1 charcoal and 1 mg L-1 KIN and the calculated averages of the best regeneration rate, the best shoot number and the best shoot length were 96.89%, 3 and 1.24 respectively on this medium. The determination of genetic stability of in vitro grown plants on the optimum medium compositions obtained by the\u0000graph kernel method was carried out with the use of the ISSR-PCR technique. All the ISSR primers produced a total of 192 reproductive band profiles, none of which were polymorphic. Furthermore, the micropropagated plants were successfully rooted and acclimatized to greenhouse conditions. In this study, we present a graph kernel multiple\u0000propagation index which considers all the possible parameters needing to be analyzed. Such an index is used for the first time for the determination of the optimum proliferation medium.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42622994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Species introductions through coconut fibre: Dactyloctenium aegyptium and Glinus oppositifolius, new records for the Balearic Islands, Spain","authors":"M. Cerrato, A. Ribas-Serra, C. Cardona, L. Gil","doi":"10.37427/botcro-2021-023","DOIUrl":"https://doi.org/10.37427/botcro-2021-023","url":null,"abstract":"Based on plant material collected in the forest nursery of the Balearic Island Forestry Center (CEFOR) for autochthonous plant production and the University of the Balearic Islands experimental facilities, two new plant records are presented for the Mediterranean island of Mallorca. Dactyloctenium aegyptium, an invasive grass previously recorded in other\u0000areas of the Mediterranean basin, and Glinus oppositifolius, a new record for the European flora. In both cases the species are presumed to\u0000have arrived through contaminated batches of the coconut fibre substrate used in both facilities.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42912429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"One new species of Cymbella C.A. Agardh (Bacillariophyta) from high altitude lakes in the Hengduan Mountains of Southwest China","authors":"QI Liu, Han Wu, YAN-LING Li, J. Kociolek","doi":"10.37427/botcro-2021-021","DOIUrl":"https://doi.org/10.37427/botcro-2021-021","url":null,"abstract":"This paper describes a new species of the genus Cymbella C.A. Agardh from an alpine lake in the Hengduan Mountains of southwestern China. A detailed morphological description of the new species, named Cymbella luoyulanae sp. nov., is presented; the description is based on light and\u0000scanning electron microscopy. The main features of C. luoyulanae are strongly dorsiventral valves with strongly reverse-lateral raphe branches near the proximal ends, a large central area occupying approximately a half of the width with the valve and slit-like areolae comprising striae that may be unseriate or partially biseriate. The new species has morphological characteristics that resemble those of C. heihainensis Y.Li et Gong, C. modicepunctata Krammer and C. asiatica Metzeltin, Lange-Bertalot et Y.Li, but it differs from these three species in details of size, valve shape, striae density, central area, and number of stigmata.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43900909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Angela G. Bartolo, G. Zammit, Hannah Russell, A. Peters, F. Küpper
{"title":"DNA barcoding of marine algae from Malta: new records from the central Mediterranean","authors":"Angela G. Bartolo, G. Zammit, Hannah Russell, A. Peters, F. Küpper","doi":"10.37427/botcro-2021-020","DOIUrl":"https://doi.org/10.37427/botcro-2021-020","url":null,"abstract":"The heterokont benthic multicellular algae Schizocladia ischiensis E.C. Henry, K. Okuda et H. Kawai (Schizocladiophyceae), Hecatonema terminale (Kützing) Kylin and Striaria attenuata (Greville) Greville (Phaeophyceae) are reported for the first time from the waters around the Maltese islands in the central Mediterranean. They were identified through algal isolation from incubated natural substrata, coupled with DNA barcoding targeting the biomarkers COI and rbcL plus the RuBisCO spacer. For three additional brown algae, Colpomenia sinuosa (Mertens ex\u0000Roth) Derbès et Solier, Asperococcus bullosus J.V.Lamouroux and Sphacelaria sp., DNA sequences confirmed previous morphology-based records from Malta. This paper also provides an updated literature-based\u0000species list of the marine macroalgae present in Malta.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46119565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First record of a naturalized population of the tropical Colocasia esculenta (Araceae) in Italy, and clarifications about its occurrence in southeastern Europe","authors":"D. Iamonico","doi":"10.37427/botcro-2021-018","DOIUrl":"https://doi.org/10.37427/botcro-2021-018","url":null,"abstract":"Colocasia esculenta (L.) Schott is an emergent aquatic and semi-aquatic species native to Asia, where it grows in tropical and subtropical areas. This species is widely cultivated for its edible corm and is considered as alien in various parts of the world, becoming sometimes invasive (e.g., in Spain), and in these areas eradication should be carried out. As part of ongoing studies on Araceae, in 2015 a population\u0000of C. esculenta was discovered in Rome (central Italy), where it grows along ditches. This is the first record of a naturalized population in Italy. A comprehensive view of this species in Italy and Europe was given, with clarifications about its occurrence in the Balkans, where C.\u0000esculenta was excluded from Bosnia and Herzegovina, Croatia, Montenegro, North Macedonia, Serbia, and Slovenia. A morphological description based on the population found and considerations of its ecology and the climatic conditions at the Roman site are provided.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45822587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Stešević, Đ. Milanović, Milica Stanišić-Vujačić, U. Šilc
{"title":"Aristida oligantha – a new alien species on the eastern Adriatic coast","authors":"D. Stešević, Đ. Milanović, Milica Stanišić-Vujačić, U. Šilc","doi":"10.37427/botcro-2021-019","DOIUrl":"https://doi.org/10.37427/botcro-2021-019","url":null,"abstract":"An alien and potentially invasive species of North American origin Aristida oligantha Michx. was found in the hinterland of Velika plaža, a\u0000sandy beach in the town of Ulcinj (Montenegro), the second known record\u0000of this plant in Europe. Here we describe the Aristida oligantha community. Further monitoring is suggested in order to evaluate its invasiveness and to plan appropriate eradication measures.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49514415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bromus diandrus (Poaceae), an addition to the Bulgarian flora","authors":"G. Kunev","doi":"10.37427/botcro-2021-017","DOIUrl":"https://doi.org/10.37427/botcro-2021-017","url":null,"abstract":"The current report discusses Bromus diandrus, a new species in the flora\u0000of Bulgaria. A concise description of its morphological features with an emphasis on the characters that distinguish it from the already known\u0000representatives of Bromus sect. Genea in the country is presented. The newly established locality is characterized floristically and ecologically. The probabilities of the native or alien origin of the studied population are also discussed.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41860572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ecophysiological study of some coastal dune species of Zemmouri El Bahri (Algeria)","authors":"Sadjia Rabhi, R. Djebbar, A. Belkebir","doi":"10.37427/botcro-2021-001","DOIUrl":"https://doi.org/10.37427/botcro-2021-001","url":null,"abstract":"Species of coastal dunes are subjected to several environmental stress factors such as drought, high temperature, light intensity as well as salinity, which led to development of specific morphological, physiological and biochemical adaptation mechanisms. Studying these strategies allows explaining the survival of these species in such hostile and stressful conditions. In this study we compared some parameters (morphological, physiological and biochemical) of two Brassicaceae species Matthiola tricuspidata (L.) W.T.Aiton, Cakile maritima Scop., and two Fabaceae species Lotus creticus L. and Ononis variegata L., harvested from their natural environment in coastal dunes of Zemmouri El Bahri (Algeria), with the aim to understand their mechanisms of adaptation. The results revealed that C. maritime showed the highest relative water content, leaf area, leaf mass area, and succulence index. Based on the highest levels of total phenols, flavonoids and anthocyanins as well as high contents of photosynthetic pigments, M. tricuspidata can be classified as \"homoiochlorophyllous\" plant. The dry mass content varied considerably among species, with the highest values observed in L. creticus and the lowest in C. maritima. As both Fabaceae species L. creticus and O. variegata showed high chlorophylls, carotenoids, total phenols and flavonoids could also be classified as \"homoiochlorophyllous\" species. The relatively high levels of total phenols, total flavonoids, anthocyanins and carotenoids implies a biochemical adaptation that allows these plants to maintain necessary photosynthetic activity under a water deficiency condition.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2021-001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42735214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Brullo, C. Brullo, S. Cambria, G. Tavilla, G. G. D. Galdo, S. Bogdanović
{"title":"Taxonomical and chorological remarks on the Mediterranean Poa maroccana (Poaceae) and the first record in Italy from the Sicilian flora","authors":"S. Brullo, C. Brullo, S. Cambria, G. Tavilla, G. G. D. Galdo, S. Bogdanović","doi":"10.37427/botcro-2021-011","DOIUrl":"https://doi.org/10.37427/botcro-2021-011","url":null,"abstract":"Poa maroccana is an annual Mediterranean species, which is recorded for the first time from Sicily and for the whole Italian territory. It usually occurs in synanthopic habitats, represented mainly by urban and suburban roadsides, where it is quite sporadic. Based on current knowledge, it has been hitherto reported only in a few localities of the Mediterranean area, particularly in Morocco, Algeria, Tunisia, Libya, Spain, Portugal, Greece, Turkey, Syria and Crimea. Field observations and herbarium investigations have shown that this species is often mistaken for P. annua, mainly due to its morphological similarities, as well as for the same ecological needs, therefore its real geographical distribution has yet to be verified. Although the two species are both tetraploids (2n = 28), they clearly differ for numerous relevant features concerning the shape and size of ligules, inflorescence, spikelet, glumes, palea, lemma, anthers, lodicules and seed. In order to better clarify the relationships of P. maroccana with P. annua and the other annual species of this genus occurring in the Mediterranean, its carefull a morphological description and iconography is provided, as well as a comparative morphological table of all allied species and also their analytical key for easy identification.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42336300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}