Acta Botanica Croatica最新文献_第10页

Drought stress response in winter wheat varieties – changes in leaf proteins and proteolytic activities 冬小麦品种的干旱胁迫反应——叶蛋白和蛋白水解活性的变化

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-07-13 DOI: 10.37427/botcro-2020-018

L. Simova-Stoilova, E. Kirova, Dobrina Pecheva

{"title":"Drought stress response in winter wheat varieties – changes in leaf proteins and proteolytic activities","authors":"L. Simova-Stoilova, E. Kirova, Dobrina Pecheva","doi":"10.37427/botcro-2020-018","DOIUrl":"https://doi.org/10.37427/botcro-2020-018","url":null,"abstract":"Radiation mutagenesis has been used in sustainable agriculture as a tool\u0000for increasing plant variability and providing new lines for selection.\u0000This necessitates a comparison, by using suitable stress markers, of the newly created lines with some well-established varieties, which are stress tolerant or susceptible. Drought is one of the most frequently encountered stresses with deleterious effects on plant performance and crop yield. Winter wheat seedlings (soil cultures at 3–4th leaf stage) from one mutant line (M181/1338K), one drought-tolerant (Guinness) and one sensitive variety (Farmer) were subjected to severe drought stress by water withholding, followed by recovery. Changes in leaf protein profiles, the amount of Rubisco large subunit (RLS), some specific chloroplast proteins such as Rubisco binding protein (RPB), Rubisco activase (RA), the chaperone subunit clpA/C of clp protease, as well as the activities of exo- and endo-proteases were analyzed. At the protein level, some differences were found in the drought response of genotypes –\u0000stability of RLS and RBP in M181/1338K and Guinness, diminution of RLS and increase in RBP in Farmer. RA presented strong up-regulation at recovery in Guinness but decreased in content under drought in M181/1338K and Farmer. Increase in ClpA/C level was found in all compared varieties under stress. Strong increase in total proteolytic activity was detected under drought only in Farmer. Inhibitory analysis revealed a predominance of cysteine and serine protease types. Aminopeptidase activities remained higher at recovery in M181/1338K and Farmer. Results are discussed in terms of genotype-linked different stress coping strategies.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2020-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44439954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Validation of variants using cost effective highresolution melting (HRM) analysis predicted from target re-sequencing in Eucalyptus 利用成本有效的高分辨率熔融(HRM)分析对桉树靶标重测序预测的变异进行验证

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-07-13 DOI: 10.37427/botcro-2020-019

A. M. Parveen, D. Lakshmanan, M. Dasgupta

{"title":"Validation of variants using cost effective highresolution melting (HRM) analysis predicted from target re-sequencing in Eucalyptus","authors":"A. M. Parveen, D. Lakshmanan, M. Dasgupta","doi":"10.37427/botcro-2020-019","DOIUrl":"https://doi.org/10.37427/botcro-2020-019","url":null,"abstract":"The advent of next-generation sequencing has facilitated large-scale discovery and mapping of genomic variants for high-throughput genotyping. Several research groups working in tree species are presently employing next generation sequencing (NGS) platforms for marker discovery, since it is a cost effective and time saving strategy.\u0000However, most trees lack a chromosome level genome map and validation of variants for downstream application becomes obligatory. The cost associated with identifying potential variants from the enormous amount of sequence data is a major limitation. In the present study, high resolution melting (HRM) analysis was optimized for rapid validation of single nucleotide polymorphisms (SNPs), insertions or deletions (InDels)\u0000and simple sequence repeats (SSRs) predicted from exome sequencing of parents and hybrids of Eucalyptus tereticornis Sm. ? Eucalyptus grandis Hill ex Maiden generated from controlled hybridization. The cost per data point was less than 0.5 USD, providing great flexibility in terms of cost and sensitivity, when compared to other validation methods. The sensitivity of this technology in variant detection can be extended to other applications including Bar-HRM for species authentication and TILLING for detection of mutants.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2020-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43853945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth inhibition of the toxic cyanobacterium Cylindrospermopsis raciborskii by extremely low-frequency electromagnetic fields 极低频电磁场对有毒蓝藻raciborski圆筒精子生长的抑制作用

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-07-10 DOI: 10.37427/botcro-2020-022

Z. Mohamed, F. Ali, M. Abdel-Lateef, A. Hosny

{"title":"Growth inhibition of the toxic cyanobacterium Cylindrospermopsis raciborskii by extremely low-frequency electromagnetic fields","authors":"Z. Mohamed, F. Ali, M. Abdel-Lateef, A. Hosny","doi":"10.37427/botcro-2020-022","DOIUrl":"https://doi.org/10.37427/botcro-2020-022","url":null,"abstract":"This study investigates the effects of extremely low-frequency electromagnetic fields (ELF-EMFs) on the growth and antioxidant defence enzymes of the toxic cyanobacterium Cylindrospermopsis raciborskii (Woloszynska) Seenayya et Subba Raju. To determine resonance frequency of growth inhibition of C. raciborskii, cells were subjected to ELF square amplitude modulated waves (QAMW) with a range of frequencies (0.1, 0.3, 0.5, 0.7, 0.9 Hz) at single intensity of 100 V m–1 for 30 minutes. The results revealed that the highest growth inhibition of Cylindrospermopsis occurred upon exposure to 0.7 Hz QAMW for 30 min. ELF-EMF-exposed cultures exhibited a marked decrease in cell number, chlorophyll-a content and activity of antioxidant enzymes compared to control cultures, and this effect increased with the prolongation of exposure time. Moreover, ELF-EMF induced morphological changes in Cylindrospermopsis cells upon exposure to 0.7 Hz QAMW for 120 min, including shrinking and disintegration of cytoplasmic contents, and thickening of the cell wall. Changes in dielectric properties, as a measure of interaction of cellular constituents (e.g., plasma membrane, cell wall and cytoplasm), with electromagnetic fields were also observed\u0000for treated cells. Our results provide a new possibility for using ELF-EMFs to eliminate toxic cyanobacteria from drinking and recreational\u0000water sources.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2020-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41453398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Phytosociological study of submontane genistoid scrub communities from the Southeastern Balkans 巴尔干东南部亚山地金雀花灌木群落的植物社会学研究

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-07-10 DOI: 10.37427/botcro-2020-017

G. Kunev, R. Tzonev, I. Tsiripidis, K. Pachedjieva

引用次数: 1

Effects of low and high irradiation levels on growth and PSII efficiency in Lemna minor L. 低、高辐照水平对柠檬草生长和PSII效率的影响。

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-05-29 DOI: 10.37427/botcro-2020-016

H. Lepeduš, Ž. Vidaković-Cifrek, Iris Šebalj, J. A. Dunić, V. Cesar

{"title":"Effects of low and high irradiation levels on growth and PSII efficiency in Lemna minor L.","authors":"H. Lepeduš, Ž. Vidaković-Cifrek, Iris Šebalj, J. A. Dunić, V. Cesar","doi":"10.37427/botcro-2020-016","DOIUrl":"https://doi.org/10.37427/botcro-2020-016","url":null,"abstract":"Plant growth and reproduction depend on light energy that drives photosynthesis. In the present study we compared growth characteristics, photosynthetic pigments content and photosystem II (PSII) performance in Lemna minor L. grown in two different irradiation regimes: low light (LL) – 50 μmolPHOTONS m-2 s-1 and high light (HL) – 500 μmolPHOTONS m-2 s-1. The main goal was to investigate the photosynthetic regulatory mechanisms that ensure adjustment to different light conditions and integrate these observations with the data on plant multiplication and biomass production. For this purpose, we measured chlorophyll (Chls) and carotenoid (Cars) contents and analyzed the energy fluxes through the PSII by saturation pulse method as well as by Chl a transient induction and JIP test. In a comparison of the effect of LL and HL on plant multiplication and fresh biomass, it was shown that the effect on growth was primarily attributed to the biomass reduction in LL while the effect on number of plants was much smaller. Total Chl and Cars contents were decreased in plants exposed to HL which indicated long-term acclimation response to the increased irradiance. Furthermore, the HL plants revealed better capability for the utilization of absorbed light in photosynthesis accompanied by photoprotective adjustment of certain number of PSII reaction centers from active to dissipative mode of functioning. In conclusion, our study showed that duckweed plants had great adjustment potential to different irradiation conditions, which might be of great importance not only under variable light availability but also when simultaneously challenged by some other environmental disturbance (e.g. different pollutants).","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2020-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47295036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The effect of salinity gradient and heavy metal pollution on arbuscular mycorrhizal fungal community structure in some Algerian wetlands 盐度梯度和重金属污染对阿尔及利亚湿地丛枝菌根真菌群落结构的影响

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-04-01 DOI: 10.37427/botcro-2020-001

W. Sidhoum, Kheira Bahi, Z. Fortas

{"title":"The effect of salinity gradient and heavy metal pollution on arbuscular mycorrhizal fungal community structure in some Algerian wetlands","authors":"W. Sidhoum, Kheira Bahi, Z. Fortas","doi":"10.37427/botcro-2020-001","DOIUrl":"https://doi.org/10.37427/botcro-2020-001","url":null,"abstract":"Algerian natural wetlands suffer from anthropogenic disturbances due to industrial development and urbanization. This study was designed to draw attention to arbuscular mycorrhizal fungi (AMF) distribution and community assemblages following heavy metal and salinity concentrations in two wetlands subjected to domestic and industrial effluents. Rhizospheric soil and roots of 18 plant species were collected in two wetlands along a decreasing salinity gradient. The results showed that 72.72% of plant species exhibit an association within arbuscular mycorrhizas (AM), and 36.36% a dual association between AM and dark septate endophytes (DSE). A total of 33 AMF morphospecies were distinguished on the basis of morphological criteria dominated by taxa belonging to Glomeraceae and Acaulosporaceae. Soil contamination was investigated by determining metallic trace elements (MTE) (Cd, Cu, Ni, Pb, Cr and Zn) using an atomic absorption spectrophotometer. Values of the pollution index revealed wetlands that were particularly polluted by lead. Two-way ANOVA showed significant variations in metal content among sampling locations and transects. Principal component analysis showed that species richness, and mycorrhizal frequency were slightly affected by MTE. This opens possibilities for their utilization in polluted soil remediation.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":"0-0"},"PeriodicalIF":1.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46174078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Morphology, anatomy, palynology and achene micromorphology of Bellis L. (Asteraceae) species from Turkey 土耳其Bellis L.（菊科）物种的形态学、解剖学、孢粉学和瘦果微形态

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-04-01 DOI: 10.37427/botcro-2020-006

Faruk Karahan

引用次数: 4

Alien water lettuce (Pistia stratiotes L.) outcompeted native macrophytes and altered the ecological conditions of a Sava oxbow lake (SE Slovenia) 外来水莴苣（Pistia stratitotes L.）击败了当地的大型植物，改变了萨瓦牛轭湖的生态条件（斯洛文尼亚东南部）

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-04-01 DOI: 10.37427/botcro-2020-009

M. Jaklič, Š. Koren, N. Jogan

{"title":"Alien water lettuce (Pistia stratiotes L.) outcompeted native macrophytes and altered the ecological conditions of a Sava oxbow lake (SE Slovenia)","authors":"M. Jaklič, Š. Koren, N. Jogan","doi":"10.37427/botcro-2020-009","DOIUrl":"https://doi.org/10.37427/botcro-2020-009","url":null,"abstract":"Introduction of an invasive alien macrophyte water lettuce (Pistia stratiotes L.) radically changed the oxbow lake in Prilipe (SE Slovenia) which has thermal springs that enables the winter survival of this tropical invader. About 10 years after the first record of P. stratiotes, the number, abundance and biomass of indigenous and non-indigenous macrophytes as well as different abiotic parameters were measured. In that period, colonized sections (~94% of the oxbow lake) were completely covered with water lettuce, and the only reservoirs of indigenous macrophyte species were the non-colonized areas (6%). Research in 2011 found only a third of the previously recorded indigenous macrophytes, but then only in small section without P. stratiotes. Three of the species that disappeared were on the Red data list. In the colonized section a higher biomass was observed than in the non-colonized section because of high abundance of water lettuce which remained the only macrophyte. Due to the presence of P. stratiotes, the intensity of light penetrating into the depth and water circulation were reduced, as was the oxygen saturation of the water. In addition to the well documented vegetative propagation of P. stratiotes, a well-established and viable seed bank has been detected in the lake sediment and after winter floods also on lake banks. In the future, special attention should be given to the thermal water ecosystems in temperate climates since they can serve as stepping stones and recruitment centres for the establishment and spread of (sub-)tropical invasive species. Facing predicted climate change such local populations of invasive species can act as stepping stones for further dispersal.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":"0-0"},"PeriodicalIF":1.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49314198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Preliminary characterization of the Quercus pubescens complex in southern Italy using molecular markers 意大利南部短毛栎复合体的分子标记初步鉴定

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-04-01 DOI: 10.37427/botcro-2020-002

R. Di Pietro, P. Di Marzio, Gaby Antonecchia, A. Conte, P. Fortini

{"title":"Preliminary characterization of the Quercus pubescens complex in southern Italy using molecular markers","authors":"R. Di Pietro, P. Di Marzio, Gaby Antonecchia, A. Conte, P. Fortini","doi":"10.37427/botcro-2020-002","DOIUrl":"https://doi.org/10.37427/botcro-2020-002","url":null,"abstract":"Quercus pubescens s.l. is a group of taxonomically intricate and highly debated deciduous white oaks widely distributed in southern Europe. The Apulia Region occupies the south-easternmost part of the Italian peninsula; the land-use pattern is based on extensive agricultural systems and only 10% is covered by forests that are mainly composed of oak woods. It is the region in Italy showing the highest number of oak species, among which four putative species of the Quercus pubescens group, have been reported in floras and checklists with uncertain taxonomic value because of the overlapping of diagnostic characters. In this paper, we carried out a molecular analysis on natural populations of Q. pubescens s.l. distributed throughout the Apulian region. Individuals from 24 pubescent oak populations were sampled and each tree was genotyped at 11 polymorphic microsatellite markers. Overall, the average expected heterozygosity (He) was 0.629, and the allelic richness (Ar) ranged between 2.130 and 7.187. No differentiation was observed among the populations investigated, and the genetic differentiation coefficient (FST) was 0.036. Gene flow among populations was found to be relatively high (Nm = 6.664). From a taxonomic point of view, the possibility of the coexistence of more than one species among the Apulian pubescent oaks reported in the taxonomic and syntaxonomic literature is not supported by the results of this molecular analysis.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44786745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

The impact of drying on bioactive compounds of blue honeysuckle berries (Lonicera caerulea var. edulis Turcz. ex Herder) 干燥对蓝金银花浆果(Lonicera caerulea var. edulis Turcz)生物活性物质的影响。牧民交货)

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-04-01 DOI: 10.37427/botcro-2020-007

M. Senica, F. Stampar, S. Ercişli, Barbara Sladonja, D. Poljuha, M. Mikulič-Petkovšek

引用次数: 5