{"title":"入侵植物龙葵内生真菌的多样性","authors":"Nigora Kuchkarova, Caixia Han, Zokir Toshmatov, Hongyang Chen, Hua Shao","doi":"10.37427/botcro-2024-001","DOIUrl":null,"url":null,"abstract":"A culture-dependent method was used to isolate fungal endophytes from the leaves, stems, and roots of the invasive plant Solanum rostratum Dunal. growing in Xinjiang Province, China. All isolates were identified according to ITS (internal transcribed spacer) region of ribosomal DNA sequences and analyzed by Nucleotide BLAST according to NCBI GenBank and Mycobank database. Altogether 176 endophytic fungal isolates corresponding to 44 OTUs were identified, which were classified into 12 genera, with Penicillium (59.66%) and Aspergillus (23.29%) being the highly dominant genera. Ten endophytic isolates (OTU1, OTU15, OTU16, OTU21, OTU23, OTU25, OTU26, OTU30, OTU37 and OTU44) were identified as potential new species.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Diversity of fungal endophytes isolated from the invasive plant <i>Solanum rostratum</i>\",\"authors\":\"Nigora Kuchkarova, Caixia Han, Zokir Toshmatov, Hongyang Chen, Hua Shao\",\"doi\":\"10.37427/botcro-2024-001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A culture-dependent method was used to isolate fungal endophytes from the leaves, stems, and roots of the invasive plant Solanum rostratum Dunal. growing in Xinjiang Province, China. All isolates were identified according to ITS (internal transcribed spacer) region of ribosomal DNA sequences and analyzed by Nucleotide BLAST according to NCBI GenBank and Mycobank database. Altogether 176 endophytic fungal isolates corresponding to 44 OTUs were identified, which were classified into 12 genera, with Penicillium (59.66%) and Aspergillus (23.29%) being the highly dominant genera. Ten endophytic isolates (OTU1, OTU15, OTU16, OTU21, OTU23, OTU25, OTU26, OTU30, OTU37 and OTU44) were identified as potential new species.\",\"PeriodicalId\":6967,\"journal\":{\"name\":\"Acta Botanica Croatica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2023-11-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Botanica Croatica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.37427/botcro-2024-001\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Botanica Croatica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37427/botcro-2024-001","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
采用培养依赖法从入侵植物龙葵(Solanum rostratum Dunal)的叶、茎和根中分离真菌内生菌。生长于中国新疆省。所有分离株根据核糖体DNA ITS (internal transcriptspacer)区序列进行鉴定,并根据NCBI GenBank和Mycobank数据库进行Nucleotide BLAST分析。共鉴定出44个OTUs对应的176株内生真菌,分12属,其中青霉菌(59.66%)和曲霉菌(23.29%)为高度优势属。10株内生菌株(OTU1、OTU15、OTU16、OTU21、OTU23、OTU25、OTU26、OTU30、OTU37和OTU44)被鉴定为潜在新种。
Diversity of fungal endophytes isolated from the invasive plant Solanum rostratum
A culture-dependent method was used to isolate fungal endophytes from the leaves, stems, and roots of the invasive plant Solanum rostratum Dunal. growing in Xinjiang Province, China. All isolates were identified according to ITS (internal transcribed spacer) region of ribosomal DNA sequences and analyzed by Nucleotide BLAST according to NCBI GenBank and Mycobank database. Altogether 176 endophytic fungal isolates corresponding to 44 OTUs were identified, which were classified into 12 genera, with Penicillium (59.66%) and Aspergillus (23.29%) being the highly dominant genera. Ten endophytic isolates (OTU1, OTU15, OTU16, OTU21, OTU23, OTU25, OTU26, OTU30, OTU37 and OTU44) were identified as potential new species.
期刊介绍:
The interest of the journal is field (terrestrial and aquatic) and experimental botany (including microorganisms, plant viruses, bacteria, unicellular algae), from subcellular level to ecosystems. The attention of the Journal is aimed to the research of karstic areas of the southern Europe, karstic waters and the Adriatic Sea (Mediterranean).