Effects of artificial sweeteners on antioxidant enzymes and physiological parameters in Triticum aestivum (Poaceae)

IF 1.1 4区 生物学 Q3 PLANT SCIENCES
Müjgan Elveren
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引用次数: 0

Abstract

Due to increased consumption, artificial sweeteners are often present in the environment but their effects on plants are largly unknown. In this research, the effects of four artificial sweeteners on plant stress markers in Triticum aestivum L. were investigated. Wheat seedlings were grown from seeds in soil containing artificial sweeteners (saccharin, sodium cyclamate, sucralose, aspartame) in different concentrations (0, 25, 50, 100 mg kg-1). Plants were irrigated at regular intervals to maintain field capacity moisture and harvested after 15 days of growth. Electrolyte leakage, chlorophyll and carotenoid content, and antioxidant enzyme (superoxide dismutase, peroxidase, catalase) activities were determined in harvested leaves. Comparisons between control samples and test samples were statistically evaluated at a 95% confidence interval to determine significant differences. Overall, significant increases in chlorophyll and carotenoid content, and some antioxidant enzyme activities were observed in wheat plants exposed to artificial sweeteners in the soil. A significant increase in electrolyte leakage was observed with saccharin and aspartame treatment, indicating that these sweeteners can cause membrane damage in wheat. Chlorophyll a nd POX activity were the most sensitive stress parameters in wheat. This study showed the importance of evaluating the potential impact of anthropogenic pollutants that may be present in treated wastewater and consequently affect plants.
人工甜味剂对小麦抗氧化酶及生理参数的影响
由于消费量的增加,人工甜味剂经常存在于环境中,但它们对植物的影响在很大程度上是未知的。本研究研究了4种人工甜味剂对小麦(Triticum aestivum L.)植株胁迫标志物的影响。小麦种子在含有不同浓度(0、25、50、100 mg kg-1)人工甜味剂(糖精、甜蜜素钠、三氯蔗糖、阿斯巴甜)的土壤中生长。植株定期灌溉以保持田间水分,生长15天后收获。测定了收获叶片的电解质泄漏量、叶绿素和类胡萝卜素含量以及抗氧化酶(超氧化物歧化酶、过氧化物酶、过氧化氢酶)活性。对照样本和测试样本之间的比较以95%置信区间进行统计评估,以确定显著差异。总体而言,土壤中添加人工甜味剂的小麦植株叶绿素和类胡萝卜素含量显著增加,抗氧化酶活性也有所增加。在糖精和阿斯巴甜处理下,电解质泄漏显著增加,表明这些甜味剂可引起小麦膜损伤。叶绿素a和POX活性是小麦最敏感的胁迫参数。这项研究显示了评估人为污染物的潜在影响的重要性,这些污染物可能存在于处理过的废水中,从而影响植物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta Botanica Croatica
Acta Botanica Croatica PLANT SCIENCES-
CiteScore
2.50
自引率
0.00%
发文量
34
审稿时长
>12 weeks
期刊介绍: The interest of the journal is field (terrestrial and aquatic) and experimental botany (including microorganisms, plant viruses, bacteria, unicellular algae), from subcellular level to ecosystems. The attention of the Journal is aimed to the research of karstic areas of the southern Europe, karstic waters and the Adriatic Sea (Mediterranean).
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