Chemistry and Physics of Lipids最新文献

{"title":"Emerging roles of Dehydrogenase/Reductase (DHRS) in cancer.","authors":"Jun Huang, Chenpeng Tang, Xue Chen, Pai Zeng, Hongde Li, Xiangjian Luo","doi":"10.1016/j.chemphyslip.2026.105590","DOIUrl":"https://doi.org/10.1016/j.chemphyslip.2026.105590","url":null,"abstract":"<p><p>As a member of short-chain dehydrogenase/reductase (SDR) superfamily, dehydrogenase/reductase (DHRS) is mainly involved in the metabolism of many substrates such as lipids, steroids and retinol. The DHRS family comprises more than 12 members, including DHRS1 - 4, DHRS6 - 13, among others. Several members have been extensively studied and implicated in tumorigenesis and cancer progression, such as DHRS2 involved in lipid metabolism, DHRS3/4/9/13 mediating all-trans retinoic acid biosynthesis, DHRS8/10/11 regulating steroid metabolism and DHRS6 maintaining intracellular iron homeostasis. Although the functions of other DHRS members remain poorly characterized, emerging evidence also suggests their association with cancer. Here, we provide an overview of the DHRS family members and highlight their roles in cancer progression.</p>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":" ","pages":"105590"},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to Lipid membrane behavior of nitro-fatty acids and their loading into liposomes to activate Nrf2 pathway in RAW264.7 cells with impact on intracellular NO production [Chem. Phys. Lipids 270 (2025) 105497].","authors":"Martina Zatloukalova, Gabin Fabre, Lukas Jedinak, Jiří Pospíšil, Damian Dziubak, Aleksandra Pavićević, Zdenek Dostal, Jiri Vrba, Slawomir Sek, Miloš Mojović, Patrick Trouillas, Jan Vacek","doi":"10.1016/j.chemphyslip.2026.105575","DOIUrl":"https://doi.org/10.1016/j.chemphyslip.2026.105575","url":null,"abstract":"","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":" ","pages":"105575"},"PeriodicalIF":2.8,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced optical methods and strategies for extracellular vesicles characterization and dynamic studies","authors":"Zike Zhang ,&nbsp;Futao Meng ,&nbsp;Chao Wang ,&nbsp;Qiao Mei","doi":"10.1016/j.chemphyslip.2026.105576","DOIUrl":"10.1016/j.chemphyslip.2026.105576","url":null,"abstract":"<div><div>Extracellular vesicles (EV) can serve as nanocarriers for drug delivery, but their clinical translation is hampered by challenges in characterizing their dynamic interactions with target cells. Moreover, the dynamic reaction between EV and target cells remains unclear, especially at single-particle level. Recent techniques in advanced microscopy and artificial intelligence (AI) now present an opportunity to overcome this bottleneck. Therefore, the goal of this review is to evaluate how these technologies can be integrated to provide a dynamic, and label-free understanding of EV biology. In this review, we discuss the properties of the EV and commonly used probes for EV tracking. We also review the latest advances in microscopic techniques for visualizing EV uptake. Furthermore, we explore the potential of AI driven image analysis from these complex processes. By emphasizing the importance of these techniques, this review aims to provide a clear roadmap for researchers to leverage these new tools and ultimately provide new perspectives for EV-based therapeutics.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"275 ","pages":"Article 105576"},"PeriodicalIF":2.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147525114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular dynamics deciphers drug-dependent stability in therapeutic liposomes","authors":"F. Rostami ,&nbsp;D.M. Aghaie ,&nbsp;O. Bavi","doi":"10.1016/j.chemphyslip.2025.105563","DOIUrl":"10.1016/j.chemphyslip.2025.105563","url":null,"abstract":"<div><div>Liposomal stability is critical for effective drug delivery, yet its dependence on drug-membrane interactions remains incompletely understood. Here, we employ atomic-scale molecular dynamics simulations to investigate the structural stability of a commercial liposome (composed of DMPC/DMPG phospholipids) when loaded with two therapeutic agents: amphotericin B (an antifungal) and 5-fluorouracil (5-FU, an anticancer drug). Our simulations reveal that amphotericin B maintains liposomal integrity, with minimal perturbations to membrane thickness, lipid order, and surface area. In contrast, 5-FU induces significant destabilization, including reduced membrane thickness, expanded lipid spacing, and disordered acyl chain packing-corroborated by alterations in deuterium order parameters, mass density profiles, and charge distributions. These findings demonstrate that while the liposome is an optimal carrier for amphotericin B, its composition requires modification for some anticancer drug delivery. The study provides mechanistic insights into drug-dependent liposomal stability, offering a framework for the rational design of tailored nanocarriers.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"274 ","pages":"Article 105563"},"PeriodicalIF":2.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145699487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of the effect of SMA derivatives on the structural topology and dynamics of two bacteriophage peptides","authors":"Nancy C. Rotich ,&nbsp;Evelyn A. Okorafor ,&nbsp;Indra D. Sahu ,&nbsp;Muhammad Zeeshan Shah ,&nbsp;Dominik Konkolewicz ,&nbsp;Gary A. Lorigan","doi":"10.1016/j.chemphyslip.2025.105562","DOIUrl":"10.1016/j.chemphyslip.2025.105562","url":null,"abstract":"<div><div>Researchers have explored and cultivated suitable membrane mimetics to preserve a physiological solvent condition for membrane protein functions. This involves emulating the properties of lipid bilayers, particularly within the hydrophobic core. Membrane mimetics exist in diverse forms, such as micelles, bicelles, liposomes, and nanodiscs. Polymers, such as styrene-maleic acid (SMA), have been found to offer a potentially suitable means to solubilize membrane proteins without resorting to detergents. It is widely recognized that various membrane mimetics yield distinct structural and dynamic configurations in membrane proteins. Styrene-maleic acid derivatives (SMADs) are of particular significance in this study; they are known for their ability to generate lipid nanoparticles. It has been hypothesized that using SMA derivatives with the same charge as the target membrane protein preserves the protein's structural and dynamic attributes compared to other bilayer membrane mimetics. This study explores the impact of different charges of SMA derivatives on two bacteriophage-encoded peptides explicitly focusing on their influence as charged peptides. Positively charged, neutral, and negatively charged SMA derivatives interactions with pinholin S²¹ and the phage-encoded cationic antimicrobial peptide gp28 lipid vesicles were assessed. These interactions were characterized using dynamic light scattering (DLS) techniques and continuous wave electron paramagnetic resonance (CW-EPR) spectroscopy. From our DLS results, we observed a reduction in size compared to the vesicle control, which is consistent with the formation of SMADLPs (styrene maleic acid derivative lipid nanoparticles). The key outcome was in the identification of how various SMA derivatives affect the interaction of gp28 and pinholin membrane peptides, which is useful when trying to understand how the different SMA polymers can influence the behavior and stability of protein complexes. For gp28 peptide, CW-EPR spectral analysis indicates no line broadening in its profile, suggesting that binding interactions with SMA derivatives do not significantly disrupt the structural integrity or dynamic behavior of the gp28 peptide. SMA-Pos interaction with pinholin shows some minimal perturbation, confirming that it is not as compatible compared to SMA-Neut and SMA-Glu. This study will provide insights into the optimal conditions for studying membrane protein interactions, focusing on the structural dynamics of gp28 and pinholin in the presence of different SMA derivatives.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"274 ","pages":"Article 105562"},"PeriodicalIF":2.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145665425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of silver nanoparticles on the morphology and membrane permeability of giant unilamellar vesicles and their mechanistic insights","authors":"Zarin Tasnim Rakhy ,&nbsp;Tawfika Nasrin ,&nbsp;Mir Jubair Ahamed ,&nbsp;Md. Masum Billah ,&nbsp;Md. Wahadoszamen ,&nbsp;Aminul I. Talukder ,&nbsp;Mohammad Abu Sayem Karal","doi":"10.1016/j.chemphyslip.2025.105564","DOIUrl":"10.1016/j.chemphyslip.2025.105564","url":null,"abstract":"<div><div>Silver nanoparticles (AgNPs) are extensively used in healthcare, medicine, and environmental fields owing to their strong antiviral and antibacterial properties. Although these NPs interact with cellular biomembranes or vesicular lipid membranes, their mechanisms of interaction under physiological conditions using cell-mimetic giant unilamellar vesicles (GUVs) have been rarely investigated. In this study, we focused on the interaction of AgNPs with cell-sized GUVs and investigated deformation, membrane permeation, and change in membrane area under 0.3 – 5.0 μg/mL concentrations of AgNPs. The synthesized particles exhibited an average size of 58.1 nm and a zeta potential of –6.9 mV. The deformation of GUV and the fraction of deformation increase with the increase of AgNPs concentration. The encapsulating calcein of GUVs leaked out through the membranes while interacting the AgNPs, indicated the nano-sized pore formation in the membranes of vesicles. The leakage constant increased with the increase of NPs concentration, as well as the pore size. The membrane area of a GUV measured by micropipette technique exhibits a dynamic response: an initial rapid expansion, followed by a gradual contraction, and a subsequent slight increase over time. This provides insights into AgNPs-GUVs binding dynamics. These investigations help to understand the mechanism of interaction of AgNPs in the cell membranes which might be used in several biophysical and biomedical applications.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"274 ","pages":"Article 105564"},"PeriodicalIF":2.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Branched-chain fatty acids with different structure exhibit distinct anti-hepatoma activities and characteristics","authors":"Yaqi Huang,&nbsp;Houyue Li,&nbsp;Jialing Gu,&nbsp;Zongrun Li,&nbsp;Weijia Bao,&nbsp;Xiaosan Wang","doi":"10.1016/j.chemphyslip.2025.105549","DOIUrl":"10.1016/j.chemphyslip.2025.105549","url":null,"abstract":"<div><div>Branched-chain fatty acids (BCFAs) exhibit potential anticancer activity, but their systematic evaluation and comparison with straight-chain saturated fatty acids (SSFAs) remain limited due to monomer accessibility issues. This study utilized lanolin, a rich BCFA/SSFA mixture, to systematically assess anti-hepatoma activities of 50 fatty acids using multiple linear regression (MLR) and orthogonal partial least squares (OPLS) models combined with HepG2 cell viability, apoptosis, and cell cycle assays. MLR identified <em>iso</em>-C13:0 as a unique protective fatty acid, while OPLS revealed strong explanatory power (R2X = 0.827–0.997, R2Y = 0.718–0.782) and key anti-hepatoma fatty acids, including SSFAs (C12:0, C13:0, C14:0, C19:0, C21:0) and BCFAs (16–19-carbon <em>iso</em>-BCFAs, 14–19-carbon <em>anteiso</em>-BCFAs). Notably, SSFAs outperformed BCFAs in certain activities, and a structure-activity trend emerged: odd-carbon BCFAs favored cell cycle arrest, even-carbon BCFAs promoted apoptosis, and 13–21-carbon fatty acids showed stronger activity. The integrated approach validated lanolin as an ideal matrix for functional lipid screening, providing a methodology to identify anticancer fatty acids in complex mixtures and challenging the conventional superiority of BCFAs.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"273 ","pages":"Article 105549"},"PeriodicalIF":2.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cholesterol and related sterols differentially modulate lipid domain dynamics in model membranes: A dual-probe analysis of domain-specific effects","authors":"Jesus Ayala-Sanmartin ,&nbsp;Antonin Lamazière","doi":"10.1016/j.chemphyslip.2025.105550","DOIUrl":"10.1016/j.chemphyslip.2025.105550","url":null,"abstract":"<div><div>The role of cholesterol in the organization and ordering of membrane domains has been well established over the past decades. However, the involvement of cholesterol precursors and byproduct sterols in modulating the physicochemical properties of cell membranes remains less thoroughly explored. In this study, we investigated the effects of cholesterol, two hydroxylated catabolites (24-hydroxycholesterol and 25-hydroxycholesterol), and two biosynthesis precursors (desmosterol and lanosterol) on model of liquid-ordered (Lo) and liquid-disordered (Ld) membrane domains. Membrane ordering and molecular mobility were assessed using two fluorescent probes; Laurdan, which senses polarity near the membrane aqueous interface and cholesterol-pyrene, which senses ordering closer to the center of the membrane bilayer. The results showed that Laurdan can distinguish between environmental polarity and the contribution of membrane domains. The probe mobility varied depending on the sterol and did not strictly correlate with membrane order. Cholesterol–pyrene revealed that the sterols induce varying degrees of ordering around the bilayer center. A notable observation in Ld membranes using different probes was that the ordering effect of sterols was similar near the lipid head groups and at the center of the bilayer. Hydroxycholesterols exhibited a low ordering effect, whereas cholesterol and desmosterol induced a strong effect. In contrast, in Lo membranes, hydroxycholesterols produced a strong ordering effect near the head groups but a reduced effect near the bilayer center.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"273 ","pages":"Article 105550"},"PeriodicalIF":2.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sources and biological functions of nervonic acid: Advances and perspectives","authors":"Yihe Li ,&nbsp;Xiaolong Gao ,&nbsp;Zhuoao Gao ,&nbsp;Zhonghong Cao ,&nbsp;Peng Xiong ,&nbsp;Xiutao Liu","doi":"10.1016/j.chemphyslip.2025.105554","DOIUrl":"10.1016/j.chemphyslip.2025.105554","url":null,"abstract":"<div><div>Nervonic Acid (NA), as a critical component of neural myelin sheaths, maintains nerve cell structural integrity and function. Due to limited synthesis in humans, it is primarily obtained through plant extraction, chemical synthesis, or biosynthetic methods. Its bioactive properties and applications encompass: mitigating oxidative stress and improving cognitive function; balancing pro-/anti-inflammatory factors to alleviate inflammation in organs such as liver or colon while modulating gut microbiota; its level fluctuations being closely associated with psychiatric disorders and metabolic diseases, demonstrating biomarker potential for early diagnosis. Furthermore, nervonic acid exerts multi-dimensional protective effects on cardiovascular health and metabolic homeostasis, while serving as a functional ingredient in dietary supplements and infant formula. This review systematically elaborates on the three primary sources of nervonic acid, discusses its biological functions in neuroprotection, anti-inflammatory activity, and metabolic regulation, and explores its potential applications in biomarker development and functional foods. The review aims to provide an important theoretical foundation for future disease prevention strategies and the development of health-oriented products.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"273 ","pages":"Article 105554"},"PeriodicalIF":2.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145426335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tucumã (Astrocaryum vulgare Mart.) oil-composed nanostructured lipid carriers for topical administration: Optimization by 22 experimental factorial design and stability assessment","authors":"Maria Eugênia B. Rocha ,&nbsp;Raquel da Ana ,&nbsp;Faezeh Fathi ,&nbsp;M. Beatriz P.P. Oliveira ,&nbsp;Leticia Kakuda ,&nbsp;Wanderley P. Oliveira ,&nbsp;Eliana B. Souto","doi":"10.1016/j.chemphyslip.2025.105548","DOIUrl":"10.1016/j.chemphyslip.2025.105548","url":null,"abstract":"<div><div>The selection of lipids and their ratios play a critical role in determining drug loading capacity and the structural properties of nanostructured lipid carriers (NLCs), directly impacting their stability. Among liquid lipids, vegetable oils have been explored both as active pharmaceutical ingredients (APIs) and as excipients in NLCs intended for topical use. The pulp oil of Tucumã, derived from Brazilian biodiversity, is known for its anti-inflammatory and antioxidant properties, attributed to its high content of carotenoids. This study focused on evaluating the compatibility of Tucumã oil with various solid lipids (SLs) commonly used in NLC production, developing an optimized NLC formulation containing this oil, and monitoring its stability over a 28-days’ period. Lipid screening was performed to assess the compatibility of Tucumã oil with a series of SLs, followed by preliminary formulations to determine the type of SL and surfactant for the experimental design. A 2² experimental factorial design was used to understand and identify the significant effects and interactions of lipid phase and surfactant concentrations on Tucumã oil-loaded NLCs, and the stability of the optimized formulation was monitored by determining the mean particle size (z-Ave), polydispersity index (PI), zeta potential (ZP), and recrystallization index (RI%) over 28 days. Compritol® was identified as the most suitable SL, resulting in round shaped NLCs with z-Ave of 309 nm, PI of 0.23 and high ZP (−25.5 mV). The RI% was shown to be influenced by the storage time and temperature. The optimal formulation contained 8 % of lipid phase (at a 20:80 ratio of oil to SL) and 3 % of Tween® 80 as surfactant, showing stability at 5ºC, 25ºC and 40ºC. The experimental factorial design revealed a positive effect of surfactant concentration on z-Ave and PI, with no significant impact on ZP. Over time, NLCs exhibited a gradual color loss (becoming whiter), with no other signs of instability. These findings support the potential use of Tucumã oil for producing stable NLCs suitable for topical delivery.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"273 ","pages":"Article 105548"},"PeriodicalIF":2.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}