Chemistry and Physics of Lipids最新文献

{"title":"Corrigendum to “Structural characterization of cholesterol-rich nanoemulsion (LDE) and associates” [Chem. Phys. Lipids 263 (2024) 105418]","authors":"Aline S. Perez , Aleksandra T. Morikawa , Raul C. Maranhao , Antonio M. Figueiredo Neto","doi":"10.1016/j.chemphyslip.2025.105498","DOIUrl":"10.1016/j.chemphyslip.2025.105498","url":null,"abstract":"","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"270 ","pages":"Article 105498"},"PeriodicalIF":3.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid membrane behavior of nitro-fatty acids and their loading into liposomes to activate Nrf2 pathway in RAW264.7 cells with impact on intracellular NO production","authors":"Martina Zatloukalova ,&nbsp;Gabin Fabre ,&nbsp;Lukas Jedinak ,&nbsp;Jiří Pospíšil ,&nbsp;Damian Dziubak ,&nbsp;Aleksandra Pavićević ,&nbsp;Zdenek Dostal ,&nbsp;Jiri Vrba ,&nbsp;Slawomir Sek ,&nbsp;Miloš Mojović ,&nbsp;Patrick Trouillas ,&nbsp;Jan Vacek","doi":"10.1016/j.chemphyslip.2025.105497","DOIUrl":"10.1016/j.chemphyslip.2025.105497","url":null,"abstract":"<div><div>Nitro-fatty acids (NO₂-FAs) are endogenous electrophilic signalling modulators, and some of them have been proposed as drug candidates. The main ones include nitro-oleic acid (NO₂-OA) and other derivatives of unsaturated fatty acids such as nitro-linoleic acid (NO₂-LA). In this study, we describe the behavior of 9/10-NO₂-OA, 10-NO₂-LA and the conjugated nitro-linoleic acid (9/12-NO₂-cLA) in a model POPC (1-palmitoyl-2-oleoyl-<em>sn</em>-glycero-3-phosphocholine) membrane using molecular dynamics and selected experimental approaches. We showed that when loaded in liposomes, NO₂-FAs undergo degradation (a decay reaction) to a very limited extent, in contrast to the free molecular form in an aqueous environment. This was confirmed by the electron paramagnetic resonance spectroscopic analysis of NO radical release. In general, NO₂-FAs suppress membrane hydration, especially in the segment where the ester groups are located. Further, in the presence of NO₂-FAs, there is increased membrane fluidity and a decrease in the degree of lipid order. These effects are greater for NO₂-FAs than for their non-nitrated versions. The presence of a nitro group in close contact with the polar head groups was confirmed. This drives the tilt of the lipid chain which in turn induces membrane disorder. Protonated NO₂-FAs penetrated more easily/deeper into the membrane structure than the dissociated forms and this makes the membrane bilayer surface more negatively charged based on zeta potential measurement. We also found that NO₂-FAs incorporated into POPC liposomes retained their ability to activate the Nrf2 pathway. This was documented by an increased expression of heme oxygenase-1 at the level of mRNA, with a parallel decrease in protein levels of Keap1, in murine macrophage RAW264.7 cells. The NO₂-FAs treatment resulted in an increase in intracellular NO level <em>in vitro</em> as determined by a genetically encoded G-geNOp sensor. This was confirmed at statistically significant level only for NO₂-OA, not for NO₂-LA or NO₂-cLA. The results indicate that biologically relevant NO release may be strictly dependent on which NO₂-FA is investigated. This study supports the hypothesis that NO₂-FAs are distributed (co-localized) in cells and tissues in the lipid or aqueous phase, which affects whether they are mobile, stable, and thus biologically active.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"270 ","pages":"Article 105497"},"PeriodicalIF":3.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the Lipid-POI connection: The mediating role of inflammatory factors","authors":"Tingyu Lang ,&nbsp;Shaoqi Hua ,&nbsp;Xiaolei Liang ,&nbsp;Yongxiu Yang","doi":"10.1016/j.chemphyslip.2025.105495","DOIUrl":"10.1016/j.chemphyslip.2025.105495","url":null,"abstract":"<div><div>POI is a highly heterogeneous, multifactorial condition, and dysregulated lipid metabolism has been implicated in its inflammatory pathogenesis This study is the first to systematically investigate causal relationships between 179 lipid species, 91 inflammatory factors, and POI using Two-Sample Mendelian Randomization (TSMR) and Multivariable Mendelian Randomization (MVMR). By integrating lipidomics and inflammatories data with POI from Genome-wide association study (GWAS) and FinnGen, we identified 18 causally significant lipids, including risk-elevating phosphatidylcholines and sphingomyelins, and protective triglycerides. Methodologically, we innovatively applied Bayesian Weighted Mendelian Randomization (BWMR) to confirm the robustness of causal estimates, addressing limitations of conventional MR in pleiotropy-prone metabolic networks. Biologically, we discovered IL-10 mediates 7.02–9.03 % of the effects of sphingomyelin (d40:2) and (d42:2) on POI, reconciling lipid-driven inflammation with ovarian aging—a mechanism previously unreported. Sensitivity analyses confirmed no horizontal pleiotropy (p &gt; 0.05). This work establishes three advances: (1) First MR evidence linking specific lipid subclasses (not just broad categories) to POI; (2) Identification of IL-10 as a novel inflammatory mediator bridging sphingolipids and POI pathogenesis; (3) A validated framework combining MVMR and mediation analysis to disentangle direct/indirect effects in reproductive aging. Our findings provide clinically actionable insights: IL-10 emerge as potential biomarkers, while triglycerides highlight dietary/therapeutic targets. This mechanistic clarity advances POI research beyond prior observational associations into causal biology.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"269 ","pages":"Article 105495"},"PeriodicalIF":3.4,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cholesterol´s role in membrane organization and nicotinic acetylcholine receptor function: Implications for aging and Alzheimer's disease","authors":"Daniel A. Peñalva,&nbsp;Juan Pablo Munafó,&nbsp;Silvia S. Antollini","doi":"10.1016/j.chemphyslip.2025.105484","DOIUrl":"10.1016/j.chemphyslip.2025.105484","url":null,"abstract":"<div><div>Biological membranes are complex entities composed of various molecules exhibiting lateral and transbilayer lipid asymmetries, along with a selective spatial distribution of different membrane proteins. This dynamic orchestration is crucial for proper physiological functions, undergoes changes with aging, and is disturbed in several neurological disorders. In this review, we analyze the impact of disruption in this equilibrium on physiological aging and the onset of pathological conditions. Alzheimer´s disease (AD) is a multifactorial neurodegenerative disorder in the elderly, characterized by the increased presence of the Aβ peptide, which supports the amyloid hypothesis of the disease. However, AD also involves a progressive loss of cholinergic innervation, leading to the cholinergic hypothesis of the disease. Nicotinic acetylcholine receptors (nAChRs) are transmembrane proteins, and Aβ peptides, their oligomeric and fibrillar species, which increase in hydrophobicity as they develop, interact with membranes. Therefore, a membrane hypothesis of the disease emerges as a bridge between the other two. Here, we discuss the impact of the membrane environment, through direct or indirect mechanisms, on cholinergic signaling and Aβ formation and subsequent incorporation into the membrane, with a special focus on the crucial role of cholesterol in these processes.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"269 ","pages":"Article 105484"},"PeriodicalIF":3.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membranes, where lipids and protein meet","authors":"Diego de Mendoza","doi":"10.1016/j.chemphyslip.2025.105486","DOIUrl":"10.1016/j.chemphyslip.2025.105486","url":null,"abstract":"<div><div>Membrane proteins have central roles in a vast number of vital cellular processes. A common structural feature of most membrane proteins is the presence of one or more hydrophobic alpha-helices that interact with the lipid bilayer. Because of the interaction with the surrounding lipids, the organization of these helices will be sensitive to lipid properties like fluidity and hydrophobic thickness. The helices may adapt to the lipids in different ways, which in turn can influence the structure and function of the intact membrane protein. In this review article, I will focus on how the lipid environment governs the signaling state of a transmembrane protein and in how the lipid bilayer influences the catalytic and substrate channeling role of a peripheral protein.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"268 ","pages":"Article 105486"},"PeriodicalIF":3.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The reverse sequence of Aβ amyloid self-triggers isolated nano-fibers and oligomers in lipid environment","authors":"Alain Bolaño Alvarez ,&nbsp;María Elisa Mariani ,&nbsp;Pablo E.A. Rodríguez ,&nbsp;Gerardo D. Fidelio","doi":"10.1016/j.chemphyslip.2025.105485","DOIUrl":"10.1016/j.chemphyslip.2025.105485","url":null,"abstract":"<div><div>Nanostructured lipid/peptide film at air/water interface allow to build different molecular arrangements depending of peptide sequence, peptide proportion and type of lipid. We studied the surface properties of Aβ(1 −42) and its retro-isomer Aβ(42 −1) amyloid peptides mixed with 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) lipid at the air/water interface. In absence of lipids, pure form of both Aβ(1 −42) and Aβ(42 −1) form insoluble monolayer films without appreciable fibril-like structures despite the high interfacial confinement. We show the lipid/peptide interfacial organization depends on the reversing sequence peptide in lipid enriched environment. In POPC/Aβ(1 −42) mixed film we have observed network fibril-like structures. However, using Aβ(42 −1) retro-isomer peptide to form the mixed film, the induced structuration acquired an isolated fibers arrangement associated with oligomers. The above structures are clearly visualized at the interface by using Brewster Angle Microscopy. In the same way, the isolate fibers and oligomers become Thioflavin T positive when they are observed by Fluorescence Microscopy. Thus, we attributed an amyloid behavior at the air/water interface that was also evidenced by Scanning Electron Microscopy when the mixed film was transferred to mica support. Changes from an exclusive β-sheet in pure peptide to a notable increase in α-helix/unordered conformations were induced by the presence of the lipid keeping with fibril-like structures. We postulated that the amyloid fibril formation at the membrane interface not only depends on the interfacial lipid environment and the low amyloid peptide content but also by the reversing sequencing that imposed a differential lipid/peptide interaction at the interface. Despite the retro-isomer peptide has not impact nor the overall molecular hydrophobicity neither on the interfacial behavior although perform a <em><strong>“conformational selective process”</strong></em> that depends on the β-sheet and α-helix contents.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"268 ","pages":"Article 105485"},"PeriodicalIF":3.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to: “Nanodisc assembly from bacterial total lipid extracts” [Chem. Phys. Lipids (2024) 105425]","authors":"Trent R. Llewellyn,&nbsp;Olivia R.C. Pimentel,&nbsp;Kiersten D. Lenz,&nbsp;Makaela M. Montoya,&nbsp;Jessica Z. Kubicek-Sutherland","doi":"10.1016/j.chemphyslip.2025.105476","DOIUrl":"10.1016/j.chemphyslip.2025.105476","url":null,"abstract":"","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"267 ","pages":"Article 105476"},"PeriodicalIF":3.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bending the rules: Molecular dynamics of hydroxylated sphingolipid membranes with 2-hydroxyoleic acid","authors":"Lucia Sessa ,&nbsp;Simona Concilio ,&nbsp;Miriam Di Martino ,&nbsp;Davide Romanini ,&nbsp;Xavier Busquets ,&nbsp;Stefano Piotto","doi":"10.1016/j.chemphyslip.2025.105475","DOIUrl":"10.1016/j.chemphyslip.2025.105475","url":null,"abstract":"<div><div>In this study, we introduce a novel method for quantifying the mechanical properties of lipid membranes-bending rigidity (κ), Gaussian rigidity (κ_G), and surface tension (γ) using molecular dynamics (MD) simulations. Our approach is applied to membranes incorporating 2-hydroxyoleic acid (2OHOA), a synthetic oleic acid derivative currently under clinical investigation for its anticancer properties. 2OHOA modifies the plasma membrane composition in cancer cells and activates sphingomyelin synthase 1 (SMS1), an enzyme critical for maintaining sphingolipid levels in the plasma membrane. This research focuses on how the integration of 2OHOA into ceramide and sphingomyelin alters the mechanical and biophysical properties of these membranes. We employed MD simulations to analyze structural parameters such as lipid area, volume, and bilayer thickness. Additionally, by constructing a system of linear equations based on the Helfrich-Seifert model, we estimated the mechanical properties of hydroxylated versus non-hydroxylated membranes. Our findings reveal significant membrane rigidity and curvature changes due to hydroxylation, affecting membrane-protein interactions and cellular processes like vesiculation. This work provides critical insights into the molecular mechanisms by which hydroxylation influences membrane elasticity, with implications for both fundamental biophysics and therapeutic applications in cancer treatment.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"268 ","pages":"Article 105475"},"PeriodicalIF":3.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined impact of pesticides on mono- and bilayer lipid membranes","authors":"Luis Fernando do Carmo Morato ,&nbsp;Gilia Cristine Marques Ruiz ,&nbsp;Carlos Junior Amorim Lessa ,&nbsp;Danilo da Silva Olivier ,&nbsp;Marcos Serrou do Amaral ,&nbsp;Orisson Ponce Gomes ,&nbsp;Wallance Moreira Pazin ,&nbsp;Augusto Batagin-Neto ,&nbsp;Osvaldo N. Oliveira Jr ,&nbsp;Carlos José Leopoldo Constantino","doi":"10.1016/j.chemphyslip.2025.105474","DOIUrl":"10.1016/j.chemphyslip.2025.105474","url":null,"abstract":"<div><div>The increased use of agrochemicals in crop production raises concerns about the risk of combined pesticide exposure through water and food, potentially leading to a ‘cocktail effect’ with synergistic impacts on human health. To investigate such effects, we used the pesticides acephate and diuron interacting with a mimetic system of the cell membrane, composed of lipid monolayers. These mimetic systems were composed by a mixture of POPC, cholesterol and sphingomyelin (70/20/10 mol%), respectively, close to the composition found in mammalian membranes. Results from Langmuir monolayers, including surface pressure-area isotherms, polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), and Brewster angle microscopy (BAM), showed that the pesticides interact predominantly with the polar head region of the lipids, a finding supported by density functional theory (DFT) calculations and molecular dynamics simulations. The cocktail had a similar effect in π-A isotherms; however, PM-IRRAS data suggest<del>s</del> a stronger effect of the cocktail on the ternary monolayer at the molecular level, once the pesticide mixture changed the conformation and orientation of the headgroup and disturbed the hydrocarbon chain. These results evidence the impact of the ‘cocktail effect’ on lipid membranes, highlighting potential health risks associated with pesticide mixtures.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"268 ","pages":"Article 105474"},"PeriodicalIF":3.4,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of biomimetic lipid membranes with detergents with different physicochemical characteristics","authors":"Mariana S.S. Oliveira,&nbsp;Amanda C. Caritá,&nbsp;Karin A. Riske","doi":"10.1016/j.chemphyslip.2025.105473","DOIUrl":"10.1016/j.chemphyslip.2025.105473","url":null,"abstract":"<div><div>Membrane solubilization by detergents is routinely performed to separate membrane components, and to extract and purify membrane proteins. This process depends both on the characteristics of the detergent and properties of the membrane. Here we investigate the interaction of eight detergents with very distinct physicochemical features with model membranes in different biologically relevant phases. The detergents chosen were the non-ionic Triton X-100, Triton X-165, C10E5, octyl glucopyranoside (OG) and dodecyl maltoside (DDM) and the ionic sodium dodecyl sulfate (SDS), cetyl trimethyl ammonium bromide (CTAB) and Chaps. Three lipid compositions were explored: pure palmitoyl oleoyl phosphatidylcholine (POPC), in the liquid-disordered (Ld) phase, sphingomyelin (SM)/cholesterol 7:3 (chol) in the liquid-ordered (Lo) phase and the biomimetic POPC/SM/chol 2:1:2, which might exhibit Lo/Ld phase separation. Turbidity measurements of small liposomes were performed along the titration with the detergents to obtain the overall solubilization profiles and optical microscopy of giant unilamellar vesicles (GUVs) was used to reveal the mechanism of interaction of the detergents. The presence of cholesterol renders the membranes partly/fully insoluble in all detergents, and the charged detergents are the least effective to solubilize POPC. The non-ionic detergents, with exception of DDM, with the bulkiest headgroup, caused a substantial increase in surface area of POPC, which was quantified directly on single GUVs. The other detergents induced mainly vesicle burst. Detergents that caused some increase in area induced Lo/Ld phase separation in the ternary mixture, with preferential solubilization of the latter. The insoluble area fraction left intact was quantified. Overall, the non-ionic detergents were the most effective in solubilizing lipid membranes.</div></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"267 ","pages":"Article 105473"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}