水合磷脂多层膜层间水的拉曼光谱表征

IF 2.8 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
E.A. Dobrynina, S.V. Adichtchev, N.V. Surovtsev
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引用次数: 0

摘要

磷脂双分子层的性质在各种生物物理和生物医学研究中都很重要,它的性质很大程度上取决于脂双分子层的水合作用。多层囊泡或平面多层囊泡中的双层水是研究界面脂水相互作用的一个非常方便的对象。然而,对于双层水的许多参数的研究还不完全,在某些情况下,不同的实验方法会得到不同的双层水参数。在这里,我们开发了一种拉曼光谱方法来表征多层磷脂样品中的双层水。该方法应用于一种饱和磷脂(DPPC)和一种不饱和磷脂(DOPC)在高相对湿度下水合,并在宽温度范围内进行了研究。研究发现,尽管在水的冰点以上,双分子层水的OH拉伸光谱与体水相似,但在最低实验温度(110 K)下,只有约五分之一的双分子层水结晶。结合已知组成的磷脂水悬浮液的拉曼光谱,确定了每个脂质分子的双层间水分子数(水合数)。在不同的温度和不同的相对湿度下,发现了水合磷脂双层的有序相(凝胶相)和无序相(流体相)的水合数。结果与其他方法得到的水化数值进行了比较,并提出了考虑自由和非自由(扰动)双层水的分数的解释。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Raman spectroscopy characterization of interbilayer water of hydrated phospholipid multibilayers
The properties of phospholipid bilayers, which are important in various biophysical and biomedical studies, critically depend on the hydration of the lipid bilayer. Interbilayer water in multilamellar vesicles or planar multilayers is a very convenient object for studying the interfacial lipid-water interaction. However, many parameters of the interbilayer water remain incompletely studied, and in some cases different experimental methods yield different parameters of interbilayer water. Here, we developed a Raman spectroscopy method for characterizing interbilayer water in multilayer phospholipid samples. This method was applied to one saturated (DPPC) and one unsaturated (DOPC) phospholipid hydrated at high relative humidity and studied over a wide temperature range. It was found that although above the freezing point of water the OH stretching spectra of interbilayer water were similar to those of bulk water, only about one-fifth of the interbilayer water crystallized at the lowest experimental temperature (110 K). In combination with Raman spectra of aqueous suspensions of phospholipids of known compositions, the number of interbilayer H2O molecules per lipid molecule (hydration number) was determined. The hydration number was found for the ordered (gel) and disordered (fluid) phases of hydrated phospholipid bilayers at different temperatures and several relative humidities. The results were compared with values of the hydration number obtained by other methods, and an interpretation was proposed that takes into account the fractions of the free and non-free (perturbed) interbilayer water.
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来源期刊
Chemistry and Physics of Lipids
Chemistry and Physics of Lipids 生物-生化与分子生物学
CiteScore
7.60
自引率
2.90%
发文量
50
审稿时长
40 days
期刊介绍: Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.
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