The puzzle of sphingolipids and cholesterol under the atomic force microscope: bilayer thicknesses and breakthrough forces

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2025-07-15 DOI:10.1016/j.chemphyslip.2025.105527

Aritz B. García-Arribas , Alicia Alonso , Félix M. Goñi

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Abstract

A variety of studies published in the last decades in the field of lipid biophysics deal with the puzzle regarding the relationship between the signaling power of bioactive lipids (sphingolipids) and their capacity to induce lipid membrane heterogeneity (domains). Advances in technology, particularly Atomic Force Microscopy (AFM), have provided a solid contribution in this regard. Moreover, supported planar bilayers (SPB) have become an established membrane model in the study of lipid-lipid interactions. However, in spite of the large amount of published results in this field, the data remain scattered, and a coherent collection that allows easy access to the investigator is missing. This review summarizes the relevant results obtained in our laboratory through the use of AFM under comparable experimental conditions, offering a collection of data on supported lipid bilayer thicknesses and breakthrough forces. An extensive list of lipid compositions including phospholipids, cholesterol and sphingolipids (sphingomyelins, ceramides), at varying molecular ratios, has been considered.

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原子力显微镜下鞘脂和胆固醇之谜：双层厚度和突破力。

在过去的几十年里，脂质生物物理学领域发表了许多关于生物活性脂质（鞘脂）的信号传导能力与其诱导脂质膜非均质性（结构域）的能力之间关系的研究。技术的进步，特别是原子力显微镜（AFM），在这方面提供了坚实的贡献。此外，支撑平面双层膜（SPB）已成为研究脂质相互作用的一种成熟的膜模型。然而，尽管在这一领域发表了大量的结果，但数据仍然是分散的，并且缺少一个便于研究者访问的连贯的收集。本文总结了我们实验室在可比实验条件下使用AFM获得的相关结果，提供了支持脂质双分子层厚度和突破力的数据集合。广泛的脂质组成，包括磷脂，胆固醇和鞘脂（鞘磷脂，神经酰胺），在不同的分子比例，已被考虑。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.