Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Treatment of nitrogen and phosphorus for four types of aquaculture wastewater by using nitrogen- and phosphorus-starved Chromochloris zofingiensis","authors":"Jiayi Li ,&nbsp;Jiawei Song ,&nbsp;Lingjiao Bu ,&nbsp;Lixin Jiao ,&nbsp;Yanmei Peng ,&nbsp;Bowen Miao ,&nbsp;Yingjie Huo ,&nbsp;Tong Li ,&nbsp;Yueqiang Guan ,&nbsp;Zhao Zhang","doi":"10.1016/j.algal.2025.104127","DOIUrl":"10.1016/j.algal.2025.104127","url":null,"abstract":"<div><div>With the gradual increase in the degree of intensification of aquaculture industry, large amounts of aquaculture wastewater (AW) with excess nitrogen (N) and phosphorus (P) are generated. In this study, the treatment feasibility of four types of AW, including the Chinese soft-shelled turtle (<em>Pelodiscus sinensis</em>) AW, the Largemouth bass (<em>Micropterus salmoides</em>) AW, the Pacific white shrimp (<em>Litopenaeus vannamei</em>) AW,<!--> <!-->and the Hybrid sturgeon (<em>Acipenser baerii</em> ♂ × <em>Acipenser schrenckii</em> ♀) AW by N- and P-starved <em>Chromochloris zofingiensis</em> was assessed. The total N concentration ranged from 14.63 mg L⁻¹ to 63.54 mg L⁻¹, while the total P concentration ranged from 0.36 mg L⁻¹ to 5.78 mg L⁻¹. The removal efficiencies of total N and total P from four types of AW ranged from 79.08 % to 97.54 % and 38.80 % to 83.02 %, respectively. In addition, <em>C. zofingiensis</em> achieved the highest protein content in <em>M. salmoides</em> AW on day 6, reaching 0.46 g g⁻¹. Where it achieved the highest fatty acids and astaxanthin content in Hybrid sturgeon AW, reaching 0.59 g g⁻¹ and 0.95 mg g⁻¹, respectively. In addition, <em>C. zofingiensis</em> exhibited a health-promoting amino acid and fatty acid composition for aquaculture animals after treatment with the four types of AW. Therefore, a recycling scheme AW treatment was proposed. Overall, this study demonstrated the feasibility of using N- and P-starved <em>C. zofingiensis</em> to convert N and P from AW into nutrients, which can be applied as aquafeed additives.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104127"},"PeriodicalIF":4.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Online multiparameter sensor for microalgae cultivation: prediction of nitrate, biomass and pigment concentrations","authors":"Yashika Sorathia ,&nbsp;Ana Pereira ,&nbsp;Aravind Krishnaswamy Rangarajan ,&nbsp;Timo van der Spek ,&nbsp;Jeroen H. de Vree ,&nbsp;Lucie Lourmière ,&nbsp;Narcis Ferrer-Ledo ,&nbsp;Joris van Nieuwstadt ,&nbsp;Marta Sá","doi":"10.1016/j.algal.2025.104123","DOIUrl":"10.1016/j.algal.2025.104123","url":null,"abstract":"<div><div>Industrial microalgae production is widely recognized as a sustainable and promising method for generating nutraceuticals, biofertilizers, food ingredients, and feed. However, the lack of automated process control in current industrial facilities is a significant limitation, primarily due to the absence of sensors capable of measuring the growth medium's chemical composition and biomass. In this study, a novel method for simultaneously measuring several critical process parameters, including nitrate, various pigments, and biomass is presented. The approach is based on optical transmission and machine learning-based chemometrics and is reagent-free and non-invasive, making it ideal for development into an automated online device.</div><div>Measurements of optical spectra and chemical concentrations were conducted for two microalgae species, <em>Arthrospira platensis</em> and <em>Tetradesmus obliquus</em>, across different levels of biomass and nitrate. Through Partial Least Squares analysis of the data, biomass concentrations can be inferred in a range up to 8 g.L⁻¹ (root mean square error (RMSE) of 0.55 g.L⁻¹ and R² 0.75 for <em>A. platensis</em>, and RMSE of 0.52 g.L⁻¹ and R² 0.88 for <em>T. obliquus</em>), using the same model for the different species (RMSE of 0.72 g.L⁻¹ and R² 0.74). Nitrate concentrations can be estimated in a range between 0 and 800 mg.L⁻¹ (RMSE of 41.70 mg.L⁻¹, R² of 0.82) in the presence of biomass and without the need to discriminate between the microalgae tested. For <em>T. obliquus</em>, total chlorophyl (from 2 to 18 mg.g⁻¹ biomass, RMSE 1.24 mg.g⁻¹ and R² of 0.71) and carotenoids content (from 1 to 12 mg.g⁻¹ biomass, RMSE 1.12 mg.g⁻¹ and R² of 0.59) can be estimated directly during the cultivation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104123"},"PeriodicalIF":4.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digestion and bioconversion of microalgal galactolipids by direct action of lipolytic enzymes on Chlamydomonas reinhardtii biomass","authors":"Beatriz Rodrigues Fachini ,&nbsp;Cassy Gérard ,&nbsp;Achille Marchand ,&nbsp;Olivier Bornet ,&nbsp;Valérie Gros ,&nbsp;Claire Vigor ,&nbsp;Camille Oger ,&nbsp;Guillaume Reversat ,&nbsp;Thierry Durand ,&nbsp;Goetz Parsiegla ,&nbsp;Brigitte Gontero ,&nbsp;Hélène Launay ,&nbsp;Frédéric Carrière","doi":"10.1016/j.algal.2025.104121","DOIUrl":"10.1016/j.algal.2025.104121","url":null,"abstract":"<div><div>Microalgae, like <em>Chlamydomonas reinhardtii</em>, are rich in polyunsaturated fatty acids (PUFAs) bound to galactolipids. Their bioaccessibility to digestive enzymes in whole cells was tested using guinea pig pancreatic lipase-related protein 2 (GPLRP2) and <em>Fusarium solani</em> cutinase, showing high lipolysis levels with total galactolipid fatty acid release of 93 % with GPLRP2. These enzymes also converted galactolipid fatty acids into ethyl esters with ethanol, what could facilitate PUFA recovery from microalgal biomass. <em>In vitro</em> digestion studies mimicking gastric and intestinal conditions revealed that human pancreatic galactolipases release α-linolenic acid (ALA) as the main free fatty acid (≥35 %). NMR analysis indicated PUFA oxidation when microalgal endogenous enzymes were not inactivated before digestion. ALA oxidation into phytoprostanes occurred, especially after lipolysis. Thus, <em>C. reinhardtii</em> galactolipids are bioaccessible to digestive enzymes, making them a valuable PUFA source, provided oxidation is controlled.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104121"},"PeriodicalIF":4.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic changes and enhanced digestibility with algae (Spirulina platensis) derived polysaccharides supplementation in broilers","authors":"Padsakorn Pootthachaya ,&nbsp;Sawitree Wongtangtintharn ,&nbsp;Warin Puangsap ,&nbsp;Nisakon Pintaphrom ,&nbsp;Theeraphat Srikha ,&nbsp;Anusorn Cherdthong ,&nbsp;Bundit Yuangsoi ,&nbsp;Bundit Tengjaroensakul ,&nbsp;Iwasaki Hironori","doi":"10.1016/j.algal.2025.104112","DOIUrl":"10.1016/j.algal.2025.104112","url":null,"abstract":"<div><div>The objective of this study was to investigate the effects of algae (<em>Spirulina platensis</em>)-derived polysaccharides (ADPs) on performance, digestibility, intestinal histomorphology, digestive enzyme activities, and metabolomic profiles in broiler chickens. A total of 320 one-day-old Ross 308 mixed-sex broilers were randomly allocated to 5 dietary treatments comprising 0 (Control), 1500, 3000, 4500, and 6000 mg ADPs/kg in a completely randomized design. Each treatment included 4 replications, with 16 birds per replicate. The feeding trial lasted 42 days, encompassing starter (1–10 days), grower (11–24 days), and finisher (25–42 days) phases. The results demonstrated that dietary ADPs supplementation at 3000 mg/kg significantly enhanced (<em>P</em> &lt; 0.05) body weight gain and improved feed conversion ratio during the overall period (1–42 days). In addition, supplementation with 1500–4500 mg/kg ADPs significantly improved (<em>P</em> &lt; 0.05) <em>in vitro</em> crude protein digestibility (period 2) and apparent metabolizable energy (periods 2 and 3). Meanwhile, <em>in vivo</em> digestibility was also enhanced by ADPs supplementation, with significant improvements (<em>P</em> &lt; 0.05) in dry matter (period 2) and crude protein (periods 2 and 3) digestibility. Histomorphological analysis revealed that ADPs supplementation at 1500–6000 mg/kg significantly increased (<em>P</em> &lt; 0.05) villus height, villus width, villus height-to-crypt depth ratio, and villus surface area throughout the small intestine. Additionally, digestive enzyme activities, including amylase, lipase, and protease, were significantly elevated (<em>P</em> &lt; 0.05) in broilers supplemented 3000–4500 mg/kg ADPs. Metabolomic analysis identified significant alterations in liver and breast muscle metabolites, with 194 differentially expressed metabolites in the liver and 134 in the breast, primarily associated with amino acid metabolism pathways. In the liver, the most affected pathways included lysine degradation, glycine-serine-threonine metabolism, and arginine biosynthesis. In the breast muscle, key pathways included phenylalanine-tyrosine-tryptophan biosynthesis, glycine-serine-threonine metabolism, and valine-leucine-isoleucine biosynthesis. Regarding principal component analysis demonstrated distinct clustering between control and ADPs-treated groups, indicating notable metabolic shifts. These findings suggest that the supplementation of ADPs from <em>S. platensis</em> enhances nutrient digestibility, intestinal morphology, digestive enzyme activity, and key metabolic pathways, ultimately supporting broiler growth performance. Based on the comprehensive findings of this study, dietary supplementation with ADPs at 3000 mg/kg is recommended as the optimal level.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104112"},"PeriodicalIF":4.6,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exopolysaccharide from Haematococcus pluvialis enhances photosynthesis, biomass and astaxanthin accumulation","authors":"Feng Chen ,&nbsp;Litao Zhang ,&nbsp;Shuju Guo ,&nbsp;Wenjie Yu ,&nbsp;Jing Li ,&nbsp;Jianguo Liu","doi":"10.1016/j.algal.2025.104120","DOIUrl":"10.1016/j.algal.2025.104120","url":null,"abstract":"<div><div><em>Haematococcus pluvialis</em> is recognized as the prime biological source of natural astaxanthin. However, the physiological and metabolic effects of <em>H. pluvialis</em> exopolysaccharide (HPEPS), which is derived from both secretion and cell wall dissolution during cell growth and transformation, remain unclear. This study systematically elucidated the regulatory effects of HPEPS on astaxanthin biosynthesis in <em>H. pluvialis</em> through physiological and metabolomics analyses, and preliminary characterization of its structure was also performed. Under high light stress, exogenous supplementation of 220 mg/L HPEPS induced cell division arrest and increased cell diameter to 1.25-fold of the original size, thereby providing space for biomass and astaxanthin accumulation. Concurrently, it enhanced photosynthetic efficiency and maintained energy metabolic homeostasis, leading to 1.36-fold, 1.82-fold, and 2.6-fold increases in biomass, astaxanthin yield, and astaxanthin content, respectively. Integrated metabolomics analysis revealed that HPEPS activation enhances carbon assimilation, photorespiration, and glyoxylate cycle pathways to enhance precursor supply for astaxanthin biosynthesis. Structural elucidation further identified HPEPS as a heteropolysaccharide primarily containing guluronic acid (GulA, 37.4 %), galactose (18.9 %), and arabinose (12.0 %). Notably, this work reports the first detection of GulA in microalgal exopolysaccharides. Overall, this study not only provides a theoretical basis for elucidating the structural composition and physiological role of HPEPS, but also develops a new approach to optimize the production of natural astaxanthin.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104120"},"PeriodicalIF":4.6,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced lead-zinc tailing remediation by inoculating cyanobacteria to induce biological soil crusts","authors":"Lianghui Hou ,&nbsp;Dongyang Li ,&nbsp;Shuhai Wan ,&nbsp;Kejun Liao ,&nbsp;Yue Tao ,&nbsp;Lanzhou Chen","doi":"10.1016/j.algal.2025.104103","DOIUrl":"10.1016/j.algal.2025.104103","url":null,"abstract":"<div><div>The high concentration of heavy metal (HM) and nutrient deficiency in tailings result in prolonged and challenging natural remediation. As the initial of ecological succession, biological soil crusts (BSCs) play a crucial role in tailing bioremediation. However, research in this area is limited. In this study, a significant increase in chlorophyll <em>a</em> content, total DNA content, and species abundance was discovered by inoculating indigenous microalgae on the surface of lead–zinc tailing sand. After 60 days of inoculation, the soil-saturated moisture content and cation exchange capacity increased significantly by 24.4 % and 11.7 %, and soil soluble salt content decreased significantly by 52.9 %. Cyanobacteria reduced the available HM in the tailings through biosorption and responded to the oxidative stress by increasing the activity of the antioxidant enzyme system. After the experimental cycle ended, soil DTPA-Pb/Zn content decreased significantly by 12.5 % and 14.3 %, while the soil soluble protein, malondialdehyde content, and activities of antioxidant enzymes (SOD, CAT, POD and TPx) showed a significant increase. The maximum photochemical efficiency (Fv/Fm) and electron transport yield (Eto/CSo) of PS II significantly decreased (0.6 and 129.1 times), and the Q_A reduction energy (TRo/RC) significantly increased (0.3 times) compared with microalgal cells. The soil concentration of C, N, and P nutrients, as well as soil enzyme activities, significantly increased in induced BSCs. The expression levels of soil N and P-cycling genes also significantly increased. Results indicate that the induced BSCs exhibit strong adaptability and excellent restorability to the lead–zinc tailing area. Our study provides a novel approach for the ecological and rapid restoration of HM tailing soil.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104103"},"PeriodicalIF":4.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel Scenedesmus sp. strain performs simultaneous nitrogen and phosphate removal from mariculture tail water at different salinities","authors":"Cong Liu ,&nbsp;Xumeng Cui ,&nbsp;Jiyao Yang ,&nbsp;Zhengmao Li ,&nbsp;Weiyun Wang ,&nbsp;Haonan Lv ,&nbsp;Hongqing Wei ,&nbsp;Xiukai Song ,&nbsp;Jun Wang","doi":"10.1016/j.algal.2025.104115","DOIUrl":"10.1016/j.algal.2025.104115","url":null,"abstract":"<div><div>Microalgae represent a highly valuable resource for the removal of nitrogen and phosphorus from aquaculture effluents. However, their biomass production and nutrient removal efficiency are significantly influenced by the limited salinity tolerance. This study successfully isolated a novel strain of <em>Scenedesmus</em> sp. with remarkable salinity adaptability. Growth analysis showed that salinity-acclimated <em>Scenedesmus</em> sp. had cell densities of 1.98 × 10⁶, 0.92 × 10⁶, and 0.65 × 10⁶ cells/mL at 0 ‰, 5 ‰, and 10 ‰ salinity after 12 days of culture, and high salinity significantly increased the contents of protein (84.58 % at 15 ‰), soluble sugar (192.20 % at 20 ‰), and total lipid (7.97-fold increase at 15 ‰) compared to freshwater condition. Moreover, photosynthetic efficiency exhibited salinity-dependent responses, with chlorophyll <em>a</em> content peaking at 274.81 % (10 ‰), while key photosynthetic parameters (YII and Fv/fm) showed 23.20–62.27 % reduction at high salinities. The microalgae could remove 72.42 % NH₄⁺-N and 94.30 % PO₄³⁻-P at 0 ‰ salinity, and 69.32 % NH₄⁺-N and 76.35 % PO₄³⁻-P at 10 ‰ salinity. Transcriptomic analysis revealed that salinity-induced differential gene expression at 10 ‰ salinity primarily affected photosynthesis-related pathways (light capture and reactions) and energy metabolism, which explains the observed decline in nutrient removal efficiency at higher salinities. The combined physiological and molecular findings provide valuable insights for applying functional microalgae in pollutant treatment of euryhaline aquaculture systems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104115"},"PeriodicalIF":4.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and biochemical characterization of Nannochloropsis oceanica IMET1 cell wall and its implications for enzyme-assisted extraction","authors":"Sylviah Khamila ,&nbsp;Sarah D'Adamo ,&nbsp;Michel H.M. Eppink ,&nbsp;Rene H. Wijffels ,&nbsp;Iulian Z. Boboescu","doi":"10.1016/j.algal.2025.104119","DOIUrl":"10.1016/j.algal.2025.104119","url":null,"abstract":"<div><div>Microalgae are considered a potential sustainable feedstock for producing ingredients used in food, feed and cosmetics. However, the rigid cell wall structure presents a significant challenge for the efficient extraction of intracellular products. Enzymatic hydrolysis of cell wall polymers could offer a mild and environmentally sustainable cell disruption approach. Nevertheless, incomplete knowledge of the structural and architectural properties of the cell wall limits the commercial application of this method. In this study, a combination of biochemical and microscopy techniques was used to decipher the composition, topography, ultrastructure and the architecture of the cell wall of <em>Nannochloropsis oceanica</em> IMET1. Acid hydrolysis of the extracted cell wall released neutral sugars, constituting 80 % of its total weight (<em>w</em>/w). Of these sugars, 64 % was glucose, which primarily constitutes the cellulose layer. This was further confirmed through calcofluor white fluorescence imaging using a scanning confocal microscope. Additionally, the presence of small amounts of arabinose, fucose, galactose, and mannose suggests the presence of polysaccharide networks connecting the outer layer, the cellulose-based layer and the plasma membrane. Scanning electron microscopy revealed a rugged cell surface covered with <em>N</em>-acetyl-glucosamine units as confirmed by fluorescent-lectin staining. Transmission electron microscopy revealed a double-layered cell wall structure. Hydrolysis of the extracted cell wall with lichenases and cellulases generated mostly glucose monomers alongside traces of other neutral sugars. Overall, this study provides insights into the complex structure of the cell wall of <em>N. oceanica</em> IMET1, which can enhance enzyme assisted extraction strategies in microalgae.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104119"},"PeriodicalIF":4.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the effect of glycerol and low-cost agricultural fertilizer for enhanced microalgae biomass production and wastewater treatment","authors":"Shatakshi Kashyap ,&nbsp;Abiraj Velusamy ,&nbsp;Diptymayee Padhi ,&nbsp;Shashi Kumar ,&nbsp;Manoranjan Nayak","doi":"10.1016/j.algal.2025.104117","DOIUrl":"10.1016/j.algal.2025.104117","url":null,"abstract":"<div><div>Microalgae may prove to be a dependable feedstock to produce value-added bioproducts if their production can be made economically viable and environmentally sustainable. This study evaluated the performance of a locally isolated microalga, <em>Chlorella</em> sp. BRE4 for biomass growth and nutrient removal efficiency in wastewater supplemented with NPK fertilizers and an organic carbon source. The maximum biomass yield was 2.91 g L⁻¹ with urea (0.75 g L⁻¹), 2.95 g L⁻¹ with single super phosphate (SSP) (0.75 g L⁻¹), and 2.83 g L⁻¹ with muriate of potash (MoP) (0.1 g L⁻¹) when each was added separately to the wastewater. In the photobioreactor (PBR), the developed wastewater-based medium was supplemented with glycerol and grown under different trophic modes. The microalga showed a maximum biomass yield of 3.16 g L⁻¹ under mixotrophy with CO₂ sparging (PBR4-MC), which was 4.93, 2.53, and 1.03 times higher than heterotrophy with simple air sparging (PBR1-HA), heterotrophy with CO₂ sparging (PBR2-HC), and mixotrophy with simple air sparging (PBR3-MA), respectively. Biochemical analysis shows that maximum lipid (106.5 mg L⁻¹ d⁻¹) and carbohydrate (94.8 mg L⁻¹ d⁻¹) productivities were achieved in PBR4-MC, being 2.8 and 7.8 times higher than under heterotrophic conditions. Moreover, the cultivation of <em>Chlorella</em> sp. in glycerol-supplemented low-cost medium (LCM) removed 92.9 % total nitrogen and 94.1 % total phosphate during the microalgal growth period of 10 days. These findings demonstrate the feasibility of integrating low-cost agricultural fertilizer and industrial by-products like glycerol in wastewater to enhance microalgal biomass and lipid production, enabling waste valorization and supporting circular bioeconomy strategies.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104117"},"PeriodicalIF":4.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Internal ploidy and heritable chromosome fragmentation in Parachlorella kessleri” [Algal. Res. 89 (2025) 104047]","authors":"Kotaro Ishii ,&nbsp;Madoka Asano ,&nbsp;Yusuke Kazama ,&nbsp;Tsuyoshi Takeshita ,&nbsp;Kateřina Bišová ,&nbsp;Vilém Zachleder ,&nbsp;Atsushi Toyoda ,&nbsp;Tomoko Abe ,&nbsp;Shigeyuki Kawano","doi":"10.1016/j.algal.2025.104108","DOIUrl":"10.1016/j.algal.2025.104108","url":null,"abstract":"","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104108"},"PeriodicalIF":4.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}