Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Differential responses of Microcystis aeruginosa to single and repeated exposure to Myriophyllum spicatum extracts","authors":"Qing Cao,&nbsp;Bensheng You,&nbsp;Haibo Xu,&nbsp;Weijing Liu,&nbsp;Shuzhan Ma","doi":"10.1016/j.algal.2025.104060","DOIUrl":"10.1016/j.algal.2025.104060","url":null,"abstract":"<div><div>Allelochemicals have been proven to be effective in inhibiting microalgae. However, there is limited knowledge regarding their effect on cyanobacteria in the case of repeated exposure, which is more environmentally relevant. In the present study, the effects of multiple low-dosage additions and single high-dosage addition of <em>Myriophyllum spicatum</em> extracts toward <em>Microcystis aeruginosa</em> were compared based on cell density, photosynthetic response, antioxidative response, and microcystins (MCs) production. Although the cumulative exposure content of <em>M. spicatum</em> extracts was the same (5 g/L), our results showed that algal growth was promoted in repeated low-dosage exposure, whereas single high-dosage exposure inhibited cell growth and decreased photosynthetic activity with the highest inhibition rate of cell density reaching 58.2 %. Oxidative stress occurred in all treatments exposed to <em>M. spicatum</em> extracts, as indicated by the increased catalase (CAT) activity and superoxide Dismutase (SOD) activity. It is noteworthy that oxidative damage to the cell membrane only occurred in single high-dosage exposure as indicated by the malondialdehyde (MDA) contents, which were 1.9, 2.1, 2.8, and 2.7 times higher than that of the control group from day 2 to day 5, respectively. Moreover, repeated low-dosage exposure could promote MC synthesis as indicated by the up-regulated <em>mcyD</em> gene abundance. Cell lysis may be responsible for the increased MCs release in single high-dosage exposure treatment since active transport of MCs was suppressed as indicated by <em>mcyH</em> gene abundance. This study revealed differential responses of <em>M. aeruginosa</em> to single and repeated exposure to allelochemicals, shedding light on the allopathy control of cyanobacteria.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104060"},"PeriodicalIF":4.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprospecting microalgal strains cultivated using bicarbonate under a pH-regulated regime for enhanced carbon capture and utilization","authors":"Mary Joy D. Latagan ,&nbsp;Dillirani Nagarajan ,&nbsp;Jih-Heng Chen ,&nbsp;Wei-Ming Huang ,&nbsp;Chun-Yen Chen ,&nbsp;Melvin R. Pacquiao ,&nbsp;Mark Daniel G. de Luna ,&nbsp;Analiza P. Rollon ,&nbsp;Jo-Shu Chang","doi":"10.1016/j.algal.2025.104064","DOIUrl":"10.1016/j.algal.2025.104064","url":null,"abstract":"<div><div>Microalgae offers significant potential as a sustainable resource for biofuel production. However, an inefficient supply of gaseous CO₂ can lead to carbon limitation due to poor mass transfer efficiency, which poses a significant challenge in large-scale microalgal cultures. This study investigates pH-stat bicarbonate-based cultivation of various microalgal strains aimed at carbohydrate and lipid production. Four green algal strains were assessed for their capability to utilize bicarbonate ions as their inorganic carbon source for photosynthesis. Among the strains, <em>Chlamydomonas</em> sp. JSC4 attained the highest biomass concentration (1.05 ± 0.05 g/L), biomass productivity (242 mg/L/day), lipid content (42.7 ± 0.85 %), and carbohydrate content (39.16 ± 0.09 %) under conditions of 50 mM KHCO₃, pH 8, and 7 days of cultivation. The biomass productivity attained in this study is higher than those in the reported literature. The predicted biodiesel quality for all strains met the ASTM <span><span>D6751</span><svg><path></path></svg></span> and EN 14214 specifications. Increasing the bicarbonate concentration to 100 mM and employing a fed-batch mode elevated the biomass to 1.45 ± 0.32 g/L, carbohydrate accumulation to 58.25 ± 1.28 % DCW, and carbon fixation efficiency to 63.8 ± 3.23 %, which is significantly higher than that of the control cultivated with CO₂ supply (0.30 ± 0.04 %). This study highlights the potential of bicarbonate ion supplementation as an effective strategy for maximal biomass production with high productivity and biomolecule accumulation, aiding in the realization of cost-effective microalgal biofuel production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104064"},"PeriodicalIF":4.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined application of nitrogen-fixing cyanobacteria enhances rice growth and nutritional quality in saline environments","authors":"Yajie Zhang ,&nbsp;Shengjie Ding ,&nbsp;Xianhui Xue ,&nbsp;Xinjuan Hu ,&nbsp;Obaid Ur Rehman ,&nbsp;Wei Liu ,&nbsp;Feifei Zhu ,&nbsp;Renxia Wang ,&nbsp;Mostafa Sobhi ,&nbsp;Feng Wang ,&nbsp;Shuhao Huo","doi":"10.1016/j.algal.2025.104061","DOIUrl":"10.1016/j.algal.2025.104061","url":null,"abstract":"<div><div>Saline soils severely affect rice cultivation due to salt stress and reduced fertility. This study investigates the potential of <em>Anabaena azotica</em> and <em>Tolypothrix tenuis</em> (nitrogen-fixing cyanobacteria) to improve the growth and quality of rice in saline environments. Potting experiments were conducted to monitor rice growth indices including plant height, chlorophyll content, and leaf area index, which are physiological parameters at all major reproductive stages (Tillering, Tasseling, and Maturity), and assessed post-harvest rice quality. The results showed that the application of cyanobacterial treatments, particularly the combination of the two species, significantly improved the physiological growth indices and overall quality of rice. Notably, under the combined treatment, plant height and leaf total chlorophyll content increased by 4.42 % and 19.46 %, respectively, compared to the control. At the early stage of transplanting, malondialdehyde (MDA) content in the treatment group with nitrogen-fixing cyanobacteria was 40.01 % lower than that of control. However, the activities of peroxidase (POD) and catalase (CAT) were significantly increased, which indicated improved stress tolerance. The nutritional quality improved due to higher protein content and increased levels of essential amino acids such as leucine, phenylalanine, lysine, and arginine. Conclusively, nitrogen-fixing cyanobacteria are a promising, green, and sustainable method for improving rice growth and quality in saline soils. The application of cyanobacteria can partially replace chemical fertilizers and restore soil health. This helps reactivate crop production capacity in degraded lands and provides an effective way to improve agricultural sustainability and resilience.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104061"},"PeriodicalIF":4.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening enzymatic extracts of Palmaria palmata based on composition and bioactivity","authors":"Sakhi Ghelichi,&nbsp;Ann-Dorit Moltke Sørensen,&nbsp;Mona Hajfathalian,&nbsp;Susan L. Holdt,&nbsp;Charlotte Jacobsen","doi":"10.1016/j.algal.2025.104048","DOIUrl":"10.1016/j.algal.2025.104048","url":null,"abstract":"<div><div>This study evaluated enzymatic extracts of <em>Palmaria palmata</em> for protein, amino acid, and phenolic contents, as well as their bioactivity including <em>in-vitro</em> antioxidant (radical scavenging and metal ion chelation), antidiabetic (α-glucosidase inhibition), and anti-obesity (porcine pancreatic lipase inhibition) properties. Three proteases including Alcalase®, Flavourzyme®, and Formea® Prime (trypsin) were applied at concentrations of 1 %, 2 %, and 3 % of protein content in biomass (% dry weight). The proteases were also investigated combined with a polysaccharidase (Celluclast®), either simultaneously or sequentially. The highest protein content and recovery were achieved when Alcalase® and Flavourzyme® were applied together (17.29 ± 0.17 % and 54.64 ± 1.53 %, respectively). Alcalase® generally produced extracts with higher protein, essential amino acids, and phenolic contents. Formea® Prime contributed to higher radical scavenging activities, with the Alcalase® and Formea® Prime combination showing the best radical scavenging (IC₅₀ = 1.04 ± 0.32 mg mL⁻¹). However, the extracts exhibited limited metal chelation activity. The best α-glucosidase inhibition was observed with Formea® Prime (35.51 ± 2.98 %) and Alcalase® (26.16 ± 2.85 %) alone at 16 mg mL⁻¹. Extracts with higher arginine content generally showed greater lipase inhibition, with the highest inhibition seen in the Alcalase® and Flavourzyme® combination (44.23 ± 0.39 %). However, this extract was low in arginine concentration, suggesting the effect might be due to peptide-polyphenol interactions. Addition of Celluclast® did not enhance the properties of the extracts and, in some cases, reduced them. Principal component analysis (PCA) indicated that radical scavenging and anti-obesity properties are likely governed by protein and phenolic contents and specific amino acids.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104048"},"PeriodicalIF":4.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing phycocyanin productivity and thermostability in Synechocystis sp. AUPL1 using Ulva lactuca hydrolysates and ulvan polysaccharides","authors":"Mohamed Gomaa,&nbsp;Shimaa Abdelmohsen Ali,&nbsp;Awatief F. Hifney","doi":"10.1016/j.algal.2025.104053","DOIUrl":"10.1016/j.algal.2025.104053","url":null,"abstract":"<div><div>Increasing the productivity and thermostability of phycocyanin (PC) are key challenges in its industrial production. This study utilized aqueous and acidic hydrolysates derived from the seaweed <em>Ulva lactuca</em> for fed-batch mixotrophic cultivation of <em>Synechocystis</em> sp. AUPL1. The acidic hydrolysate contained higher levels of nutrients and reducing sugars (0.08 mg mL⁻¹) compared to the aqueous hydrolysate (0.03 mg mL⁻¹). Supplementing the cyanobacterial culture with 3 % (v/v) acidic hydrolysate every three days significantly increased biomass productivity, PC content, and PC productivity, achieving approximately 2-, 3-, and 6.5-fold improvements, respectively, compared to the autotrophic control. Additionally, ulvan polysaccharide, extracted from the same seaweed, was tested as a natural preservative to prevent PC degradation at elevated temperatures. Ulvan at 3 % (w/v) significantly extended the PC half-life at 60 °C to 293.21 min, compared to 101.65 min in the control. Thermodynamic analysis confirmed the thermostabilizing effect of the PC-ulvan mixture, revealing that the thermal degradation process was endothermic and non-spontaneous. This study underscores the potential of <em>U. lactuca</em> biomass as a low-cost, sustainable nutrient source for enhancing PC productivity, while also demonstrating that ulvan effectively improves PC thermostability.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104053"},"PeriodicalIF":4.6,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-cost cocultivation of Vischeria stellata and Tribonema sp. biomass using molasses vinasse: A novel wastewater reuse model for functional aquatic feed production","authors":"Yuexin Sun ,&nbsp;Yu Jiang ,&nbsp;Jinghua Pang,&nbsp;Yuxuan Zhang,&nbsp;Yuying Deng,&nbsp;Tianche Zhao,&nbsp;Peihong Shen,&nbsp;Luodong Huang","doi":"10.1016/j.algal.2025.104051","DOIUrl":"10.1016/j.algal.2025.104051","url":null,"abstract":"<div><div>Microalgae can effectively sequester CO₂, cultivating dense algal populations and producing high-value biomass in acidic environments poses significant challenges. This study explored the growth of unicellular <em>Vischeria stellata</em> GXU-A13 and filamentous <em>Tribonema</em> sp. GXU-A10 in acid molasses vinasse (MV). Results revealed that GXU-A13 exhibited strong acid tolerance, with maximum biomass reaching 2.71 g/L in MV wastewater (pH 5) and a COD removal rate of 49.4 % after 12 days. Notably, a mixed culture of the easily harvested GXU-A10 and acid-resistant GXU-A13 demonstrated enhanced wastewater treatment, with COD removal rates of 64.3 % and NH₄-N removal rates of 54.9 %. Additionally, the GXU-10 and GXU-A13 co-culture also achieved efficient biomass recovery, and examined the effects of this biomass as feed on the growth and immune function of <em>Oreochromis niloticus</em>. Feeding <em>O. niloticus</em> with 2.5 % or 5 % GXU-A13 (V2 and V5 group) resulted in significant improvements in specific growth rate (SGR). Additionally, GXU-A13 significantly enhanced glutathione peroxidase (GSH-PX) activity and decreased malondialdehyde (MDA) levels, which contributed to a marked increase in intestinal microbial diversity. Furthermore, diets containing 2.5 % GXU-A13 (V2 group) and 2.5 % GXU-A10 (H2 group) exhibited stronger antioxidant capacity and immune response. The dual metabolic benefits of mixed algae (VH group) that contained 2.5 % GXU-A13 and 2.5 % GXU-A10 significantly improved intestinal and liver health, while enhancing <em>O. niloticus</em> tolerance to high-ammonia nitrogen wastewater (NH₄⁺ concentration of 114 ± 3.2 mg/L). This study effectively integrates the unique characteristics of the two microalgae, enabling MV purification and recycling, advancing the development of a microalgae-based circular economy, and enhancing the quality and sustainability of aquatic feed.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104051"},"PeriodicalIF":4.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting chemical toxicity towards Raphidocelis subcapitata with quantum chemical descriptors","authors":"Xinliang Yu","doi":"10.1016/j.algal.2025.104055","DOIUrl":"10.1016/j.algal.2025.104055","url":null,"abstract":"<div><div>The green alga <em>Raphidocelis subcapitata</em> is an important model organism in toxicity studies. This paper, for the first time, reported classification models for <em>Raphidocelis subcapitata</em> toxicities (pEC₅₀ and pEC₁₀), by applying 43 quantum chemical descriptors and the random forest algorithm. Both classification models for the toxicities pEC₅₀ and pEC₁₀ have prediction accuracy values of 100 % for the training set (251 organics) and above 90 % for the test set (83 organics). The quantum chemical descriptors selected include atomic charges, multipole moments, frontier orbital energies and kinetic energies, polarizability and thermochemistry properties. They are related to reaction sites, electrophilic and nucleophilic reactivity, chemical bond formation, hydrogen bonds and electrostatic force. The two classification models based on a sufficiently large sample set provide an important tool for assessing the toxicity categories of organics towards <em>Raphidocelis subcapitata</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104055"},"PeriodicalIF":4.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing lutein concentration in an indigenous microalgal strain through salinity, light intensity and nutrient concentrations and evaluation of its anticancer potential","authors":"Arima Marchese ,&nbsp;Serena Lima ,&nbsp;Valeria Villanova ,&nbsp;Eleonora Montuori ,&nbsp;Daniele De Luca ,&nbsp;Chiara Lauritano ,&nbsp;Francesca Scargiali","doi":"10.1016/j.algal.2025.104054","DOIUrl":"10.1016/j.algal.2025.104054","url":null,"abstract":"<div><div>The search for new approaches for treating cancer is an urgent need and microalgae have recently shown to be an underestimated source of biocompounds. In this work, the anticancer potential of an indigenous <em>Chlorella-</em>like microalgal strain was investigated by optimizing its lutein content using a Design of Experiment (DoE) approach. Several parameters were assessed such as NaCl, phosphate and nitrate concentration in combination with light intensity. Sodium chloride and nitrate concentrations showed antagonist effects on lutein accumulation. The crude methanolic extracts of the obtained microalgal cultures, containing 2.15 ± 0.12, 1.70 ± 0.17 and of 0.68 ± 0.09 μg mg⁻¹ of lutein, were tested on cancer cell lines, revealing a dosage-dependent antiproliferative effect on melanoma A2058 cells. The extracts' fractions containing lutein exhibited similar effects, likely due to a concerted action of multiple substances, with lutein being one of the main contributors. The described approach, which combined bioprocess optimization and assessment of anticancer proprieties, showed a significant potential in the discovery of new bioactives for pharmaceutics.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104054"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of algal strains for carbohydrate production: Potentials of Chlorella lewinii FwC1 under different trophic modes and in open-pond conditions","authors":"Chandrani Debnath ,&nbsp;Tarun Kanti Bandyopadhyay ,&nbsp;Muthusivaramapandian Muthuraj","doi":"10.1016/j.algal.2025.104050","DOIUrl":"10.1016/j.algal.2025.104050","url":null,"abstract":"<div><div>Microalgae-based carbohydrates have been identified as a potential feedstock for third-generation biofuel production, but with several limitations from strain selection to mass production. Thus, identifying an appropriate microalgal cellular factory for mass-scale production of carbohydrates is essential. The present study screened eight algal strains with a multi-criteria approach, identifying <em>Chlorella lewinii</em> FwC1 as the most effective, with carbohydrate productivity of 19.76 mg L⁻¹ day⁻¹ and biomass yield of 0.15 g mol⁻¹ photon under photoautotrophic conditions. This strain thrived across various temperatures (24–36 °C) and pH (5.5–10.5). Under mixotrophic conditions with glucose, it achieved maximum biomass and carbohydrate productivity of 414 and 208 mg L⁻¹ day⁻¹, respectively, significantly surpassing heterotrophic and photoautotrophic growth. Sodium bicarbonate supplementation enhanced biomass productivity by ∼2-fold under photoautotrophic and 1.67-fold under mixotrophic conditions. Further, optimization of photoautotrophic condition improved biomass and carbohydrate productivity to 190 and 64.6 mg L⁻¹ day⁻¹, respectively, with a biomass yield of 0.38 g mol⁻¹ photon. Under open-pond conditions at a 300 L scale with optimal media composition, productivity reached 100.2 mg L⁻¹ day⁻¹ for biomass and 34.5 mg L⁻¹ day⁻¹ for carbohydrates. Thus, <em>Chlorella lewinii</em> FwC1 is a promising candidate for carbohydrate production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104050"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of adaptive laboratory evolution for improving polyunsaturated fatty acid biosynthesis in Aurantiochytrium sp. through enhancing high-glucose adaptability","authors":"Zhipeng Li ,&nbsp;Danling Tang ,&nbsp;Lifen He ,&nbsp;Yuanzhen Song ,&nbsp;Wei Hang ,&nbsp;Huipeng Liu ,&nbsp;Guoxiang Chi ,&nbsp;Tao Hong ,&nbsp;Hui Ni ,&nbsp;Qingbiao Li ,&nbsp;Zhen Chen ,&nbsp;Ning He","doi":"10.1016/j.algal.2025.104039","DOIUrl":"10.1016/j.algal.2025.104039","url":null,"abstract":"<div><div><em>Aurantiochytrium</em> sp. (<em>Schizochytrium</em> sp.) is a marine fungi-like heterotrophic single-celled eukaryote utilized for the production of polyunsaturated fatty acids (PUFAs). Adaptive Laboratory Evolution (ALE) is an effective method to improve PUFAs yield. The 20th generation evolutive strain (ALE-HG20) with high PUFAs yield was screened out after high-glucose of ALE for 180 days. The yields of total lipid, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) of ALE-HG20 strain increased by 15.51 %, 225.37 % and 52.67 % in fed-batch fermentation, respectively. Fermentation kinetics showed that the synthesis of total lipids and DHA had a tendency of uncoupling with cell growth in ALE-HG20 strain, while the synthesis of EPA had a tendency of deepening coupling with cell growth. Transcriptomic analysis revealed that the upregulation of membrane transport function genes led to rapid glucose absorption, thereby accelerating the glycolytic pathway to provide carbon skeleton for PUFAs synthesis. The metabolites of TCA pathway increased, but the amino acid anabolism associated with TCA pathway decreased, which further promoted the polysaturated fatty acid synthesis pathway. Meanwhile, the precursor metabolites of PUFAs synthesis were significantly increased. This might also be due to the protein structure evolution of GAPD and IS from glycolytic pathway and PUFAs synthesis pathway in ALE-HG20. The active pockets of GAPD and IS were increased by 10.36 % and 98.20 %, respectively, and hydrogen bonds were increased by 11.76 % and 132.76 %. This study clarified the change of regulated and evolved mechanism of PUFAs synthesis by ALE.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104039"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}