Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Integrated approach for wastewater remediation and biofertilizer development using Chlorella vulgaris in raceway ponds","authors":"Daniela Delgado Pineda ,&nbsp;Mery Y. Flores Guetti ,&nbsp;Ximena Rosado-Espinoza ,&nbsp;Muhammad Ajaz Ahmed ,&nbsp;Gilberto J. Colina Andrade ,&nbsp;Ruly Terán Hilares","doi":"10.1016/j.algal.2025.104022","DOIUrl":"10.1016/j.algal.2025.104022","url":null,"abstract":"<div><div>Wastewater is quite complex and requires a series of different processes for its treatment. Vermifilters are one such strategy that significantly reduces pollutants but with limited ability to remove phosphorus and nitrogen. To address this, the effluent from the vermifilters can be further processed by microalgae, which can enhance water quality. In this study, microalgae were cultivated in an outdoor raceway pond, using treated wastewater as medium, and the resulting biomass was used as a biofertilizer for Cherry tomato (<em>Solanum lycopersicum</em> var. <em>cerasiforme</em>) cultivation. The microalgae treatment led to significant reductions of 78 % in COD, 86 % in phosphorus, and 45 % in nitrogen (<em>p</em> &lt; 0.05). A microalgae concentration of 1.45 g/L was achieved, with a productivity rate of 0.43 g/L/day. The microalgae biomass contained 56.17 % protein, 30.73 % carbohydrates, and 18 % lipids. After 90 days of growth, cherry tomato plants fertilized with the microalgae-based biofertilizer produced a total weight of 622.4 g, compared to 756.5 g with chemical NPK fertilizer (20-20-20) and 285.4 g with no fertilizer (the control). In terms of other growth metrics such as average height, dry biomass, and number of leaves; the plants treated with commercial fertilizer reached the best results, following by the microalgae biofertilizer and finally, the control showed the least growth. These results suggest that microalgae-based biofertilizer can be a promising alternative, particularly for sandy loam soils, which are usually employed for tomato cultivation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104022"},"PeriodicalIF":4.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiochemical insights into substrate-diatom adhesion: Influence of surface properties on biofilm dynamics and productivity","authors":"Manikandan Sivakumar ,&nbsp;Inbakandan Dhinakarasamy ,&nbsp;Clarita Clements ,&nbsp;Naren Kumar Thirumurugan ,&nbsp;Subham Chakraborty ,&nbsp;Anu Chandrasekar ,&nbsp;Jeevitha Vinayagam ,&nbsp;Kumar Chandrasekaran ,&nbsp;Thirugnanasambandam Rajendran ,&nbsp;S.A.S. Suriyaraj ,&nbsp;K.R. Arjun Aditiya","doi":"10.1016/j.algal.2025.104024","DOIUrl":"10.1016/j.algal.2025.104024","url":null,"abstract":"<div><div>Diatoms play a significant role in biofilm formation due to their unique surface properties and robust extracellular polymeric substance (EPS) production. This study investigates the influence of substrate and diatom surface physiochemical parameters on adhesion and biofilm productivity. Using <em>Amphora coffeiformis</em> COR_3 and <em>Nitschzia microcephala</em> COR_2 as a model organism, results highlighted that the adhesion density varied significantly with substratum type. The strongly polar <em>N. microcephala</em> COR_2 cells (γ^p = 32.5 ± 0.89 mJ/m²) favored increased adhesion towards glass with adhesion density of 3.017 × 10⁵ cells/mm². Conversely, <em>A. coffeiformis</em> COR_3 with its intense dispersive component (γ^LW = 24.1 ± 0.08 mJ/m²) promoted strong adhesion towards PE with adhesion density of 2.938 × 10⁵ cells/mm². <em>A. coffeiformis</em> favored adhesion with hydrophobic substrates via van der Waals interactions, facilitated by hydrophobic methylene groups on its surface. While, <em>N. microcephala</em> promoted adhesion with the hydrophilic substrates through acid-base interactions and hydrogen bonding. These findings are further complemented by the varying EPS composition with <em>A. coffeiformis</em> producing protein-rich EPS on hydrophobic substrates, while <em>N. microcephala</em> releasing carbohydrate-rich EPS on hydrophilic substrates. This surface chemistry driven biofilm formation provides a baseline for optimizing the diatom-substrate pairs to enhance biofilm performance in industrial applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104024"},"PeriodicalIF":4.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay between Ca2+ and ROS in regulating β-carotene biosynthesis in Dunaliella salina under high temperature stress","authors":"Jianxin Tang,&nbsp;Zizhou Zhang,&nbsp;Jinghan Wang,&nbsp;Fantao Kong,&nbsp;Zhanyou Chi","doi":"10.1016/j.algal.2025.104023","DOIUrl":"10.1016/j.algal.2025.104023","url":null,"abstract":"<div><div>Temperature influences biomass and β-carotene production in <em>Dunaliella salina</em>, while high temperature generally induces β-carotene biosynthesis via increased ROS, the intricate and incomplete mechanisms necessitate the identification of novel regulators and their interactions with ROS. A comprehensive analysis of physiological responses to different temperatures was investigated in this study, including cell proliferation, biomass growth, carbon utilization efficiency, β-carotene synthesis, chlorophyll profiles, photosynthetic efficiency, ROS, antioxidant content, activity of antioxidant enzymes, free radical scavenger capacity, and Ca²⁺ level. The results showed that 34 °C is the optimal temperature for β-carotene accumulation, and high temperature impairs chlorophyll, photosynthesis, and carbon utilization by enhancing oxidative stress. High temperature (40 °C vs 25 °C) triggers a simultaneous increase in ROS (24.34-fold) and Ca²⁺ levels (16.04-fold), implicating Ca²⁺ as a temperature-sensitive regulator of β-carotene that potentially interacts with ROS. Further chemical treatments at 34 °C demonstrated that Ca²⁺ supplementation enhanced biomass and increased β-carotene yield and content by 4.81 %, 11.79 %, and 19.99 %, respectively. Supplementation with H₂O₂ increased the yield and content of β-carotene (5.62 % and 30.57 %) but reduced biomass (19.23 %). Notably, supplemented H₂O₂ decreased cellular Ca²⁺, while supplemented Ca²⁺ enhanced antioxidant capacity. This study identifies Ca²⁺ as a novel regulator of high-temperature β-carotene biosynthesis and shows its interplay with ROS.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104023"},"PeriodicalIF":4.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of a novel polysaccharide from Tribonema minus and preparation of polysaccharide–selenium nanoparticles with enhanced antioxidant activity","authors":"Rundong Yang ,&nbsp;Yuhao Guo ,&nbsp;Yanchao Mao ,&nbsp;Wenxiang Zhang ,&nbsp;Feifei Wang ,&nbsp;Zhenzhou Zhu","doi":"10.1016/j.algal.2025.104020","DOIUrl":"10.1016/j.algal.2025.104020","url":null,"abstract":"<div><div><em>Tribonema</em> polysaccharide (TP), a biologically active polysaccharide containing β-1,3-glycosidic bonds, has not been extensively studied as a dispersing agent for stabilising selenium nanoparticles (SeNPs), and its synergistic effects with SeNPs remain poorly understood. This study investigates the relationship between the antioxidant activity and physicochemical properties of TP–SeNPs, which were synthesised through chemical reduction at varying TP-to‑selenium mass ratios (TPSMRs). TP was isolated from <em>Tribonema minus</em> using ultrasonic-assisted extraction, achieving a 119.64 % increase in yield under optimal conditions (400 W, 25 min, 1:400 solid-to-liquid ratio). Structural analysis identified TP as a novel β-1,3-glucan (8.46 kDa) composed exclusively of glucose units. Moreover, TP–SeNPs prepared at varying TPSMRs exhibited significantly higher antioxidant activity than either TP or SeNPs alone, demonstrating a clear synergistic effect. Notably, TP–SeNPs prepared at a TPSMR of 2:3 exhibited the lowest half-maximal inhibitory concentrations for scavenging 2,2-diphenyl-1-picrylhydrazyl, superoxide anions, and hydroxyl radicals (0.30, 0.77 and 0.46 mg/mL, respectively). This was attributed to the increased selenium content and reduced particle size of these complexes. Additionally, TP–SeNPs displayed excellent dispersibility and stability, likely due to non-covalent interactions between TP and SeNPs. In cellular assays, TP–SeNPs exhibited low cytotoxicity and dose-dependently enhanced cell viability under H₂O₂-induced oxidative stress. Cells treated with 200 mg/L TP–SeNPs exhibited a 35.83 % increase in viability compared with the model group. This protection was associated with elevated antioxidant enzyme activity, increased glutathione levels and reduced malondialdehyde content, thereby mitigating oxidative damage in HepG2 cells. This study is the first to characterise a novel β-1,3-glucan from <em>T. minus</em> and to elucidate the structure–activity relationships of TP–SeNPs, highlighting their potential as functional additives or pharmaceutical supplements.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104020"},"PeriodicalIF":4.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription factor MYB15 mediates docosahexaenoic acid biosynthesis and salt tolerance in Schizochytrium sp.","authors":"Yana Liu,&nbsp;Xiao Han,&nbsp;Pengyu Pan,&nbsp;Zhi Chen","doi":"10.1016/j.algal.2025.104016","DOIUrl":"10.1016/j.algal.2025.104016","url":null,"abstract":"<div><div><em>Schizochytrium</em> sp., a unicellular heterotrophic marine protist belonging to the thraustochytrids, serves as a commercial source of docosahexaenoic acid (DHA). Enhancing strain tolerance to environmental stress promotes strain robustness and productivity. Currently, we know very little about the transcription regulators that regulate DHA biosynthesis and abiotic stress response in thraustochytrids, which hinders strain improvement by transcription factor engineering strategies. In this study, we identified a 3R-MYB transcription factor, MYB15, which was transcriptionally downregulated under low-nitrogen condition in <em>Schizochytrium</em> sp. ATCC20888. Deletion of the <em>myb15</em> gene increased production of total lipids and DHA by 27.1% and 46.7%, respectively. Under high salt condition, <em>myb15</em>-deletion mutant ΔMYB15 displayed stronger salt tolerance and higher biomass than parental strain or complementation strain CMYB15. Electrophoretic mobility shift assays (EMSAs), DNase I footprinting assays, and transcriptome analysis demonstrated that MYB15 bound specifically to the 6-bp element 5′-CNGTTR-3′ in upstream regions of target genes, represses transcription of fatty acid biosynthetic genes (<em>dga1</em>, <em>fabD</em>, <em>fas</em>, and <em>pfa</em>) and salt tolerance-related genes (<em>sos2</em>, <em>sos3</em>, and <em>betA</em>). Taken together, we demonstrated that MYB15 acts as a critical repressor for DHA biosynthesis and salt stress response. Deletion of <em>myb15</em> generates a mutant with remarkable capabilities of DHA production and salt resistance, which make it a promising <em>Schizochytrium</em> strain for industrial application.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104016"},"PeriodicalIF":4.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental and model-based parameterization of the fundamental process kinetics of Chromochloris zofingiensis","authors":"Yob Ihadjadene ,&nbsp;Lory Ascoli ,&nbsp;Tehreem Syed ,&nbsp;Leon Urbas ,&nbsp;Thomas Walther ,&nbsp;Gunnar Mühlstädt ,&nbsp;Stefan Streif ,&nbsp;Felix Krujatz","doi":"10.1016/j.algal.2025.104012","DOIUrl":"10.1016/j.algal.2025.104012","url":null,"abstract":"<div><div>The microalgae <em>Chromochloris zofingiensis</em> has attracted considerable interest due to its remarkable ability to accumulate high value metabolites. Current research focuses primarily on laboratory-scale cultivation, while the development of fundamental model frameworks for process upscaling is neglected. In order to develop an effective scale-up process for <em>C. zofingiensis</em>, it is crucial to identify the strain-specific kinetics and dependencies on the environmental factors affecting its growth (light, temperature, pH, nutrients and dissolved oxygen concentration), as many of them remain unknown. This study represents a combination of different experimental and model-based approaches to parametrize the key growth parameters of <em>C. zofingiensis</em>, as well as its response kinetics towards different cultivation factors for model framework construction and calibration. The optimal temperature, DOC and PAR intensity for photosynthesis were successfully determined to be 26.58 °C, 6–7 mg_O₂/L, and 22.42 μmol/(m² s), respectively. This enabled the development of a model-based control strategy, by maintaining the average PAR intensity within the PBR at its optimal range of 20–30 μmol/(m² s), to ensure an efficient scale-up process, which effectively improved the productivity by a factor of 5 when scaling up the cultivation of <em>C. zofingiensis</em> to a 200 L pilot-scale PBR.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104012"},"PeriodicalIF":4.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfiltration of microalgae biomolecules: Impact of pulsed electric fields and high-pressure homogenization on single-cell protein recovery and membrane fouling","authors":"Byron Perez ,&nbsp;Zhao Li ,&nbsp;Iris Haberkorn ,&nbsp;Joseph Dumpler ,&nbsp;Alexander Mathys ,&nbsp;Peter Desmond","doi":"10.1016/j.algal.2025.104015","DOIUrl":"10.1016/j.algal.2025.104015","url":null,"abstract":"<div><div>This study investigated the separation of single-cell proteins from disrupted <em>Auxenochlorella protothecoides</em> microalgae using microfiltration. The microalgae were heterotrophically cultivated and subjected to two cell disruption methods, high-pressure homogenization (HPH) and pulsed electric field with incubation (PEF + inc), and stored at −20 °C. The impact of HPH and PEF + inc on the microfiltration performance of thawed samples was assessed. HPH treatment resulted in complete cell lysis, releasing a heterogeneous mixture of intracellular compounds. This, potentially combined with freezing effects, led to rapid membrane fouling and a 70 % decline in the permeate flux, finally stabilizing at 15 L m⁻² h⁻¹. In contrast, PEF + inc preserved cell integrity and particle size even after freezing, liberating mainly soluble compounds. This resulted in a more porous cake layer and a limited decline in the permeate flux, which stabilized at 24 L m⁻² h⁻¹. Additionally, higher protein transmission rates were achieved with PEF + inc (141.75 g m⁻² h⁻¹) than with HPH (54.53 g m⁻² h⁻¹) and it was 5.6 times more energy efficient. The cake layer formed by the HPH-treated samples had a higher resistance owing to smaller apparent pores and increased interaction with permeating proteins, which hindered protein transmission. These findings demonstrate that cell disruption methods can significantly influence downstream protein recovery efficiency and energy consumption. PEF + inc shows great potential as an energy-efficient bioprocessing method for microalgae biorefineries.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104015"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key nutrients and their transportation processes influencing algal growth in a dam-controlled river","authors":"Jing Liu ,&nbsp;Qiuyu Liu ,&nbsp;Huaibin Wei ,&nbsp;Xinyu Zhang ,&nbsp;Wenhan Wan ,&nbsp;Yanmei Wang","doi":"10.1016/j.algal.2025.104017","DOIUrl":"10.1016/j.algal.2025.104017","url":null,"abstract":"<div><div>A dam-controlled river refers to a river where flow and water levels are regulated through hydraulic engineering structures, such as dams and sluices. This research focuses on controlling eutrophication in dam-controlled rivers, with particular attention to the Huaidian Dam on China's Shaying River. The primary objective of this research was to identify the key nutrients and their transportation processes affecting algal growth during the non-flood season. To achieve this, we analyzed algal density, species diversity, and the distribution of key nutrients, including ammonia nitrogen (NH₄⁺-N), nitrate nitrogen (NO₃⁻-N), and total phosphorus (TP) across dissolved, suspended, and sediment phases in both the hyporheic zone and surface water. The results reveal that diatoms dominate the algal community, comprising 66.91 % of the total composition and displaying higher cell density and biomass. Nutrient concentrations were mainly observed downstream of the dam, with NH₄⁺-N predominantly detected in sediments (2.92–9.23 mg/kg) and pore water (1.1–13.5 mg/L), NO₃⁻-N in sediment (2.5–12 mg/kg), and TP mainly in sediments (50–70 mg/kg). Redundancy analysis demonstrated that NO₃⁻-N in overlying water, sediment, and pore water were critical for algal growth, contributing 78.9 %, 77.5 %, and 75 %, respectively. Our findings suggest that the interaction between NO₃⁻-N transport and transformation within sediments and pore water is a significant factor influencing algal growth during non-flood seasons. This study highlights the influence of nutrient transport and transformation at the water-sediment interface for algal growth and recommends targeted measures to mitigate eutrophication risks in dam-controlled rivers.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104017"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel culture method with filter paper for isolating aerial microalgae under aerial-phase conditions","authors":"Nobuhiro Aburai,&nbsp;Yudai Ishikura,&nbsp;Katsuhiko Fujii","doi":"10.1016/j.algal.2025.104019","DOIUrl":"10.1016/j.algal.2025.104019","url":null,"abstract":"<div><div>Terrestrial microbial mats contain a wide variety of aerial microalgae, including useful drug-resistant and non-drug-resistant algal strains. A method for cultivating aerial microalgae in diverse terrestrial environments is needed to isolate these useful strains. The aim of this study was to develop a novel method for isolating aerial microalgae that does not rely on conventional antibiotics and characterize the isolated strains by screening and species identification. The effectiveness of filter paper as a culture substrate was investigated under aerial conditions; a total of 79 strains of aerial microalgae were successfully isolated from the microbial mat. After screening strains that grew well by altering the nitrogen source, pH, cultivation temperature, and light intensity of the medium, nine promising strains were identified from the isolated strains. Molecular phylogenetic trees suggested that algal strains were aerial microalgae of the genera <em>Coelastrella</em>, <em>Klebsormidium</em>, <em>Tetratostichococcus</em>, and <em>Chloroidium</em>. This simple, filter paper-based isolation method is thus effective for isolating aerial microalgae of various genera.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104019"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colony formation of toxic Microcystis aeruginosa affected by microplastics-derived organic matter","authors":"Cunhao Du ,&nbsp;Wenlu Sang ,&nbsp;Yushen Ma ,&nbsp;Hui Chen ,&nbsp;Xiaoming Zhu ,&nbsp;Lixiao Ni ,&nbsp;Chu Xu ,&nbsp;Jiahui Shi ,&nbsp;Muyu Feng ,&nbsp;Mohamed Abbas ,&nbsp;Yiping Li ,&nbsp;Shiyin Li","doi":"10.1016/j.algal.2025.103997","DOIUrl":"10.1016/j.algal.2025.103997","url":null,"abstract":"<div><div>Cyanobacterial blooms and microplastic (MP) pollution have become increasingly prominent global environmental issues. This study investigated the effects of polyethylene-derived organic matter (PE-DOM) and polylactic acid-derived organic matter (PLA-DOM) on the growth and physiological characteristics of <em>Microcystis aeruginosa</em> (<em>M. aeruginosa</em>). Fluorescence spectroscopy revealed that both PLA-DOM and PE-DOM contained tryptophan-like compounds that promoted the growth of <em>M. aeruginosa</em>, with PLA-DOM exhibiting higher concentrations of these compounds. Elemental analysis indicated that both MPs-DOM provided essential trace elements such as Mg, Na, Ca, and Zn, which are crucial for algal growth. Both MPs-DOM treatments increased cell biomass, chlorophyll-<em>a</em> content, polysaccharide and protein contents, and the activities of catalase (CAT) and superoxide dismutase (SOD), while also improving photosynthetic parameters. These results suggest that MPs-DOM foster <em>M. aeruginosa</em> growth by enhancing nutrient availability, photosynthetic efficiency, and stress tolerance. Overall, <em>M. aeruginosa</em> showed better growth at higher concentrations of DOM and better growth under exposure to PLA-DOM. Furthermore, MPs-DOM enhanced the expression of <em>mcy</em>B and <em>mcy</em>D, leading to the production and release of microcystin-LR (MC-LR), which promoted the formation of dominant <em>M. aeruginosa</em> populations. These findings provide new insights into the formation of cyanobacterial blooms and the ecological risks posed by MP pollution.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 103997"},"PeriodicalIF":4.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}