Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Microfluidic-assisted T-junction internal gelation for phycocyanin encapsulation","authors":"Onnicha Rueangkrachai ,&nbsp;Boonpala Thongcumsuk ,&nbsp;Sukunya Oaew ,&nbsp;Sarawut Cheunkar","doi":"10.1016/j.algal.2025.104242","DOIUrl":"10.1016/j.algal.2025.104242","url":null,"abstract":"<div><div>Phycocyanin (PC), a high-value bioactive compound widely used in the food, cosmetic, and pharmaceutical industries, faces critical limitations due to its structural instability under external environments. To address these challenges, we present a microfluidic-assisted encapsulation strategy that offers significant advantages over conventional encapsulation techniques in terms of precision, uniformity, and structural control. This study introduces a surfactant-free internal gelation process within a T-junction microfluidic device, enabling in situ formation of calcium-alginate capsules via Ca-EDTA crosslinking. The method achieved highly uniform spherical capsules with an average diameter of 1.272 ± 0.025 mm, a high encapsulation efficiency of 89.19 ± 0.31 %, and a phycocyanin loading of 9.20 ± 0.19 % using an optimized formulation of 8 %(w/v) Ca-EDTA and 2 %(w/v) sodium alginate. Furthermore, chitosan coatings at varying concentrations were applied to modulate capsule stability and release behavior, showing enhanced protection in simulated gastric conditions and controlled release in intestinal environments. Compared to traditional bulk gelation or emulsion-based methods, this microfluidic platform offers superior control over capsule morphology and release kinetics without the need for surfactants or organic solvents. This work advances the field of algal biotechnology by providing a scalable, biocompatible, and tunable encapsulation system that significantly improves phycocyanin stability and delivery potential for functional food and drug delivery applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104242"},"PeriodicalIF":4.5,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermentation of cultivated brown algae Saccharina latissima and Alaria esculenta and effects on alginate quality","authors":"Mina Gravdahl ,&nbsp;Øystein Arlov ,&nbsp;Inga Marie Aasen ,&nbsp;Finn L. Aachmann","doi":"10.1016/j.algal.2025.104241","DOIUrl":"10.1016/j.algal.2025.104241","url":null,"abstract":"<div><div>Alginate, the main structural component of brown algae, is a family of polysaccharides containing <span><math><mi>β</mi></math></span>-<span>d</span>-mannuronate (M) and <span><math><mi>α</mi></math></span>-<span>l</span>-guluronate (G) in varying ratios. Most alginate produced outside of Asia comes from wild harvested biomass, whereas cultivated seaweeds can provide a complementary supply to existing and new markets. Unfortunately, cultivated brown algae have a short harvest season, and cost-effective and sustainable preservation methods are required to ensure year-round access to high-quality biomass for alginate production. This study examines the effects of lactic acid fermentation for up to 95 days of cultivated <em>Saccharina latissima</em> (SL) and <em>Alaria esculenta</em> (AE) on alginate yield and quality in terms of purity, molecular weight (M_w) and monosaccharide composition. Alginate yield was relatively stable throughout storage time. Alginate lyase activity was observed in all the stored AE samples, while only in the SL alginate stored for 95 days. Both species showed a decrease in alginate M_w, presumably mainly due to acid hydrolysis during storage, but for AE, the decrease in M_w was also due to lyase activity. Enzymatic activity resulted in the AE alginate G fraction increasing from 53 % to 65 %, while the SL alginate G fraction remained constant during storage. The overall findings demonstrated that fermentation had a stabilizing effect on brown algae, maintaining a sufficiently high quality of the alginates for several applications. Rapid decrease in pH is crucial to prevent unwanted microbial growth, as well as lyase activity, but pH should not be too low, to avoid excessive depolymerization by acid hydrolysis.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104241"},"PeriodicalIF":4.5,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Light Emitting Diode (LED) irradiation on growth, biochemical, antioxidant, and antibacterial activity of Scenedesmus sp","authors":"Hoda A. Fathey ,&nbsp;Asmaa H. Al-Badwy ,&nbsp;M. Hassan ,&nbsp;Shaimaa M.I. Alexeree ,&nbsp;Amal A.A. Mohamed","doi":"10.1016/j.algal.2025.104236","DOIUrl":"10.1016/j.algal.2025.104236","url":null,"abstract":"<div><div>Different LED wavelengths, red at 635 nm, blue at 447 nm and mixed wavelength irradiation either sequential red-blue or simultaneous red-blue for different exposure times (30, 60 and 90 min) were investigated for their effect on the growth, cell size, pigment accumulation, total carbohydrate and protein content of the micro alga, <em>Scenedesmus</em> sp. Bioactive secondary metabolites including phenolics, flavonoids, saponins and tannins were estimated. In addition, antioxidant and antibacterial activities of methanolic algal extract were detected. Exposure to 30 min of red wavelength and 60 min in case of blue and mixed wavelengths exhibited maximum growth of <em>Scenedesmus</em> sp. Exposure duration for 90 min to different wavelengths decreased cell size of <em>Scenedesmus</em> sp. as compared with non-treated cells. In addition, accumulation of chlorophyll <em>a</em>, b, and carotenoids was in the following order: M (sequential red-blue) &gt; R + B (simultaneous red-blue) &gt; blue &gt; red &gt; control. A noticeable increase in total carbohydrate content was observed in all treatments, except, the blue wavelength treatment (B) in comparison to the control. The maximum protein content was recorded under control and blue LED illumination. Flavonoid content was markedly increased in all treatments compared to the control however, phenolic content was significantly reduced. Exposure of <em>Scenedesmus</em> sp. to blue LED for 60 min was positively linked to the enhancement of bioactive antioxidants. Exposure of <em>Scenedesmus</em> sp. to red LED for 30 min was linked to strong antibacterial activity. Further research is required to elucidate the underlying mechanisms governing algal responses to specific wavelengths.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104236"},"PeriodicalIF":4.5,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation of gametophyte culturing and seeding methods for cultivation of the kelp Ecklonia radiata","authors":"Rebecca J. Lawton ,&nbsp;Marie Magnusson","doi":"10.1016/j.algal.2025.104240","DOIUrl":"10.1016/j.algal.2025.104240","url":null,"abstract":"<div><div>Identification of optimal gametophyte culturing and seeding methods is essential for the development of kelp aquaculture for novel target species. We investigated the effects of light colour (red, white), light intensity (5–60 μmol photons m⁻² s⁻¹) and initial gametophyte density (0.005–0.25 g DW L⁻¹) on gametophyte growth and reproductive success in <em>Ecklonia radiata</em>; and the effects of seeding density (0.025–0.090 mg DW m⁻¹ twine), biomass state (vegetative gametophyte, microscopic sporophyte), and seeding method (spray, spray+rub, xanthan binder, alginate binder) on sporophyte density, growth and attachment strength on seeding twine in three laboratory experiments. We found pronounced and significant differences in the growth and reproductive success of <em>E. radiata</em> gametophytes in free floating cultures maintained under different light colours, light intensities and stocking densities. We also found substantial and significant differences in sporophyte density, growth and attachment strength among seeding methods, seeding densities and biomass states for hatchery seeding of twine. Based on our results we recommend maintaining gametophyte cultures under red light at an intensity of 20 μmol photons m⁻² s⁻¹ and a density of 0.025 g DW L⁻¹ to maximise vegetative growth, while successful reproduction can be induced within two weeks by placing cultures under white light at 20 μmol photons m⁻² s⁻¹ and a density of 0.005–0.05 g DW L⁻¹. For hatchery seeding and cultivation, we recommend spraying vegetative gametophyte biomass onto seeding twine at a density of 0.090 mg DW m⁻¹ twine. There was no advantage to using binders or inducing reproduction in biomass prior to seeding.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"92 ","pages":"Article 104240"},"PeriodicalIF":4.5,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanochemical synthesis of N-doped porous cyanobacteria-based biochar for decontamination of antibiotic wastewater","authors":"Wei Liu ,&nbsp;Jie Deng ,&nbsp;Ting Zhou ,&nbsp;Gaoping Xu ,&nbsp;Zewei Liu ,&nbsp;Yue Chang ,&nbsp;Ronghan Wang ,&nbsp;Chengyun Zhou ,&nbsp;Qihong Liu","doi":"10.1016/j.algal.2025.104238","DOIUrl":"10.1016/j.algal.2025.104238","url":null,"abstract":"<div><div>N-doped porous <em>cyanobacterial</em>-based biochar (N-PBC) was synthesized via ball milling coupled with pyrolysis and demonstrated as a high-performance adsorbent for enrofloxacin (ENR) removal. Compared to raw biochar (BC, 25.06 mg g⁻¹), N-PBC exhibited a significantly enhanced adsorption capacity of 51.02 mg g⁻¹ (adsorbent dosage 0.05 g L⁻¹, 298 K), corresponding to removal efficiencies of 22.48 % and 12.17 % for N-PBC and BC, respectively. Adsorption kinetics for both biochar followed the pseudo-second-order model, suggesting chemisorption-dominated mechanisms, while isotherms aligned with the Langmuir model, indicating monolayer adsorption. Thermodynamic analysis confirmed the spontaneous and endothermic nature of ENR adsorption. N-PBC maintained stable adsorption capacity across varying pH conditions and in the presence of common anions, alongside excellent regeneration performance. This study innovatively transforms <em>cyanobacterial</em> waste into functional N-doped biochar, showcasing its superior design for active sites and surface area. The findings underscore <em>cyanobacterial</em> waste biochar significant potential as a sustainable, efficient adsorbent for antibiotic remediation in water treatment applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104238"},"PeriodicalIF":4.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae inoculation increases bacterial diversity and gene abundances related to nutrient removal while decreasing antibiotic resistant genes in municipal wastewater","authors":"Muneer Ahmad Malla ,&nbsp;Faiz Ahmad Ansari ,&nbsp;Jonathan Featherston ,&nbsp;Humeira Hassan ,&nbsp;Magray Owaes ,&nbsp;Aaliyah Osman ,&nbsp;Anna Heintz-Buschart ,&nbsp;Nico Eisenhauer ,&nbsp;Arshad Ismail ,&nbsp;Faizal Bux ,&nbsp;Sheena Kumari","doi":"10.1016/j.algal.2025.104217","DOIUrl":"10.1016/j.algal.2025.104217","url":null,"abstract":"<div><div>Monitoring the interactions between microalgae and microbiomes, particularly in the context of nutrient and antibiotic resistance genes (ARGs) removal from municipal wastewater treatment system, is highly recommended for the environment and public health. This study evaluated the synergistic potential of microalgae-bacteria consortia in enhancing nutrient removal and mitigating ARGs in a lab-scale microalgal municipal wastewater treatment system. Three treatments were evaluated: the two individual microalgal strains (<em>Tetradesmus obliquus</em> and <em>Chlorella sorokiniana</em>) and their mixtures (1:1). All treatments showed high removal efficiencies for phosphates (&gt;90 %; <em>p</em> &lt; 0.001), nitrates (&gt;80 %, <em>p</em> &lt; 0.001), ammonia (&gt;90 %, <em>p</em> &lt; 0.0004) and chemical oxygen demand (COD) (&gt;65 %, <em>p</em> &lt; 0.008), significantly improving water clarity and quality. Microbiome analyses revealed that microalgal inoculation altered bacterial community diversity, structure and function. Functional metagenomic profiling showed a significant enrichment (<em>p</em> &lt; 0.05) of genes associated with nitrogen and phosphorus metabolism in microalgal treatment groups compared to the untreated control. Resistome analysis indicated that raw wastewater harbored a high abundance of ARGs, which were significantly reduced following microalgal treatment (<em>p</em> &lt; 0.0001). Overall, our findings demonstrate a mutually beneficial interaction between microalgae and the wastewater microbiome, where microalgae-induced changes increase bacterial diversity and functional gene abundance involved in nutrient and ARG removal. These results underscore the potential of microalgae-microbiome-based systems as an effective and sustainable solution for the removal of emerging contaminants, including nutrients, ARGs and pathogens from municipal wastewater.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104217"},"PeriodicalIF":4.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic magnetite nanoparticles for the magnetic separation of microalgae","authors":"Igor Taveira ,&nbsp;Rogerio Presciliano ,&nbsp;Júlia Castro ,&nbsp;Tarcisio Correa ,&nbsp;Rafael Richard ,&nbsp;Leonardo Brantes Bacellar Mendes ,&nbsp;Fernanda Abreu","doi":"10.1016/j.algal.2025.104237","DOIUrl":"10.1016/j.algal.2025.104237","url":null,"abstract":"<div><div>The biotechnological potential of microalgae is significant in various industrial sectors, but inefficient and expensive harvesting techniques hinder its widespread use. Regarding cell separation and concentration, few works used magnetic materials to harvest the cells. In general, the interaction between the mineral particles and cells is greatly influenced by pH and salinity conditions, which can limit their range of applications. Biogenic magnetite nanoparticles (BMs) produced by magnetotactic bacteria have unique magnetic properties and surface chemistry that can be applied for microalgae harvesting. In our experimental procedure, it was defined that 80 μg BMs·mL⁻¹ under a more acidic pH was ideal for the process with cell density ranging from 3·10³ to 1,2·10⁴ cells/mL. At pH 3.1, it was possible to magnetically concentrate up to 80.08 ± 2.08 % and 75.26 ± 2.45 % of <em>Scenedesmus</em> sp. and <em>Desmodesmus</em> sp. biomass, respectively. BMs also showed a reasonable recovery rate (52.08 %) when challenged under hypersaline conditions (40 g NaCl·L⁻¹) at pH 3.1 for <em>Nannochloropsis</em> sp. In this condition, synthetic magnetite nanoparticles (SMPs) and flocculants are ineffective in biomass harvesting. Thus, this work describes the process and efficiency of a magnetic nanotool capable of magnetically concentrating microalgae for downstream applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104237"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of the xanthophyll cycle improves biomass productivity and light tolerance of the microalga Nannochloropsis oceanica","authors":"Tim Michelberger ,&nbsp;Eleonora Mezzadrelli ,&nbsp;Britt Rietbroek ,&nbsp;Sarah D'Adamo ,&nbsp;Giorgio Perin ,&nbsp;Marcel Janssen ,&nbsp;Tomas Morosinotto","doi":"10.1016/j.algal.2025.104232","DOIUrl":"10.1016/j.algal.2025.104232","url":null,"abstract":"<div><div>Microalgae in photobioreactors encounter highly diverse light conditions along the culture depth because of cellular self-shading. Culture mixing also causes individual cells to rapidly shift from darkness to strong illumination, causing oversaturation of the photosynthetic apparatus and damage. Moreover, travelling from high light to darker layers can lead to energy loss through photoprotective mechanisms that remain active even when not needed.</div><div>In the microalga <em>Nannochloropsis oceanica</em> the xanthophyll cycle plays a central role in the regulation of light harvesting and photoprotection, making it a central target for the optimization of photosynthetic performances in photobioreactors. Recently, we showed that the genetic control of the accumulation of enzymes that regulate the xanthophyll cycle, violaxanthin de-epoxidase (VDE), and zeaxanthin epoxidase (ZEP), can dramatically accelerate its kinetics.</div><div>In this study, we characterized the impact of VDE and ZEP on the growth of <em>N. oceanica</em>. We monitored biomass productivity, pigment content, and photosynthetic performances, comparing single with double over-expressors in limiting and excess light conditions, in both tubular and flat panel photobioreactor systems.</div><div>The acceleration of the xanthophyll cycle activation by increased accumulation of VDE led to increased photoprotection and enhanced light tolerance, but also excessive energy dissipation in limiting light. The enhancement of the xanthophyll cycle relaxation through ZEP accumulation, instead, reduced energy losses but could also increase photosensitivity. The combination of the two approaches resulted in the greatest benefit, leading to an improved productivity across different photobioreactor geometries and light conditions.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104232"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new method for the online determination of mass transfer and oxygen production rates in microalgae raceway reactors","authors":"R. Arraga ,&nbsp;N.A. Dambruin ,&nbsp;M. Barceló-Villalobos ,&nbsp;M. Janssen ,&nbsp;F.G. Acién","doi":"10.1016/j.algal.2025.104235","DOIUrl":"10.1016/j.algal.2025.104235","url":null,"abstract":"<div><div>In this study, a novel methodology for the real-time determination of mass transfer coefficient and oxygen production rates in large-scale raceway reactors for microalgae cultivation was developed. An innovative approach based on dissolved oxygen (DO) measurements and controlled air pulse injections provided accurate and real-time insights into the photosynthetic performance of microalgae cultures and the efficiency of gas exchange mechanisms within the reactor. Experiments were performed in 80 m² raceway reactors under continuous operation. A set of controlled aeration pulses at different flow rates (125, 250, 375, and 500 L/min) is applied throughout the day, enabling the evaluation of oxygen production under varying light intensities. The method is designed to operate without disrupting culture stability and ensures continuous monitoring of mass transfer dynamics in raceway reactors. The optimal conditions for the utilization of the proposed methodology are defined, and the robustness and accuracy of results are validated. Results demonstrate that oxygen production rates directly correlate with solar radiation, following a linear trend that serves as a proxy for biomass productivity. The study confirms that the sump section plays a crucial role in oxygen desorption, with mass transfer coefficients reaching up to 200 h⁻¹ at maximal superficial gas velocities below 0.02 m/s, then operating in a homogeneous aeration regime. This research provides a powerful tool for optimizing large-scale microalgae production systems, enabling real-time performance monitoring and dynamic process control to enhance biomass productivity.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104235"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy-metal-resistant characterization of Salinimicrobium maris sp. nov. potential significance in algal-bacterial interactions with Phaeocystis globosa","authors":"Fei Li ,&nbsp;Lian Yu ,&nbsp;Mingben Xu ,&nbsp;Zhe Li ,&nbsp;Caibi Lan ,&nbsp;Qiu-Xia Yang ,&nbsp;Jun-Xiang Lai","doi":"10.1016/j.algal.2025.104234","DOIUrl":"10.1016/j.algal.2025.104234","url":null,"abstract":"<div><div>In heavy metal-contaminated environments, algal-bacterial interactions enhance metal detoxification while reinforcing stress resilience and ecological remediation capacity. However, whether such interactions extend to harmful algal blooms remains unexplored. In this study, we investigated a novel bacterial strain GXAS 041^T isolated from <em>Phaeocystis globosa</em> bloom in Beibu Gulf. Polyphasic taxonomic analysis confirmed its classification within the genus <em>Salinimicrobium</em>. Functional assays revealed the isolate was resistant to heavy metal stress in decreasing order Pb (1250 μM), Zn (750 μM), Cu (500 μM), Cr (400 μM), and Cd (250 μM). Langmuir adsorption isotherms revealed maximum biosorption capacities of 1.67 (Cd), 5.35 (Cr), 4.32 (Cu), 4.11 (Pb), and 6.15 (Zn) mg/g, with mechanisms characterized by SEM-EDS and FTIR analyses. The strain also produced siderophores and IAA, suggesting synergistic roles in metal chelation and stress mitigation. Crucially, binary culture with strain GXAS 041^T increased cell density of <em>P. globosa</em> by 10.1–60.2 % and reduced bioavailable metals by 4.2–32.8 % versus axenic algae. These findings reveal a dual role of <em>Salinimicrobium</em> sp. in metal detoxification and algal growth promotion, providing new insights into phycosphere symbiosis under environmental stress.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104234"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}