An efficient method for producing polysaccharides with high antioxidant activity from Caulerpa lentillifera and evaluating their stability

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-06-16 DOI:10.1016/j.algal.2025.104153

Ratana Chaiklahan, Nattayaporn Chirasuwan, Thanyalak Srinorasing, Channarong Suaisom

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引用次数: 0

Abstract

Algal polysaccharides are widely utilized in various industries due to their functional and bioactive properties. However, commercial-scale polysaccharide production requires simple, efficient, and low-cost processes. This study developed a practical and scalable method for producing polysaccharides from Caulerpa lentillifera. Given its high moisture and polysaccharide content, a squeezing method followed by spray drying was evaluated. The squeezed fresh polysaccharide (SFP) exhibited higher levels of bioactive compounds and stronger antioxidant activity than the polysaccharide extracted at 90 °C (90-FP). Similarly, the spray-dried polysaccharide (SDP), obtained from squeezing and spray drying, showed superior bioactive compound content and antioxidant activity compared to the ethanol-precipitated polysaccharide (EPP). The SDP extract contained 51.25 % total sugars, 7.33 % sulfates, 2.62 % uronic acid, 7.99 % protein, and 8.64 mg GAE/g total phenolic content. It exhibited strong antioxidant activity, with 50 % inhibitory concentration (IC₅₀) values of 0.73 mg/mL for 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, 9.57 mg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 26.96 mg/mL for lipid peroxidation (LPO) inhibition, and 0.88 mg/mL for ferrous ion-chelating (FIC) ability. Its ferric-reducing antioxidant power (FRAP) was 7.49 mg Fe²⁺/g extract. Additionally, the SDP extract demonstrated high stability suitable for industrial application, with a shelf life of 8.78 months and a half-life of 57.76 months at room temperature.

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以小扁豆为原料制备高抗氧化多糖的有效方法及稳定性评价

藻多糖因其功能性和生物活性而被广泛应用于各个工业领域。然而，商业规模的多糖生产需要简单、高效和低成本的工艺。本研究开发了一种实用、可扩展的从扁豆中提取多糖的方法。考虑到其水分和多糖含量高，采用挤压后喷雾干燥的方法进行了研究。与90°C （90- fp）提取的多糖相比，鲜榨多糖（SFP）具有更高的生物活性化合物水平和更强的抗氧化活性。同样，通过挤压和喷雾干燥得到的喷雾干燥多糖（SDP）与乙醇沉淀多糖（EPP）相比，具有更高的生物活性化合物含量和抗氧化活性。总糖含量为51.25%，硫酸盐含量为7.33%，尿酸含量为2.62%，蛋白质含量为7.99%，总酚含量为8.64 mg /g。具有较强的抗氧化活性，对2,2′-氮基-双（3-乙基苯并噻唑-6-磺酸）（ABTS）自由基的清除能力为0.73 mg/mL，对2,2-二苯基-1-吡啶酰肼（DPPH）自由基的清除能力为9.57 mg/mL，对脂质过氧化（LPO）的抑制能力为26.96 mg/mL，对铁离子螯合（FIC）的清除能力为0.88 mg/mL。其铁还原能力（FRAP）为7.49 mg Fe2+/g提取物。此外，SDP提取物具有较高的稳定性，适合工业应用，室温下的保质期为8.78个月，半衰期为57.76个月。

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment