Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Indigenous microalgae consortium for producing safe reclaimed water and bio-fertilisers. Batch tests for replication, scale-up and legislative analysis","authors":"J. González-Camejo ,&nbsp;A.L. Eusebi ,&nbsp;F. Fatone ,&nbsp;M. Pachés","doi":"10.1016/j.algal.2025.104043","DOIUrl":"10.1016/j.algal.2025.104043","url":null,"abstract":"<div><div>This study aimed to assess the potential of using indigenous microalgae to treat sewage and produce safe reclaimed water and bio-fertilisers. Cultivation tests were conducted using sole or mixed sewage streams combining primary effluent (PSE), secondary effluent (SSE), and centrate (CEN) to identify the most suitable substrate. Overall, higher nitrogen removal rates, biomass productivity, and carbon biofixation were observed when microalgae were cultivated in PSE compared to SSE, with values ranging from 2.1 to 12.1 mgN·L⁻¹·d⁻¹, 18 to 52 mgVSS·L⁻¹·d⁻¹ (20–53 mgTSS·L⁻¹·d⁻¹), and 10.7 to 27.0 mgCO₂·L⁻¹·d⁻¹, respectively. Adding centrate improved microalgae performance by increasing the availability of nutrients and inorganic carbon, but when 30 % centrate was added, detrimental effects were observed. The costs of the microalgae-based treatment process ranged from 0.081 to 0.098 €·m⁻³, which is significantly lower than those of conventional activated sludge (CAS) processes. The reclaimed water obtained during the tests demonstrated high-quality in terms of pathogen and metal levels, making it suitable (in Spain) for agricultural, recreational, industrial and environmental uses. However, some legal gaps were identified regarding the use of reclaimed water and microalgae biomass for bio-fertiliser production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104043"},"PeriodicalIF":4.6,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Effect of temperature, nitrate concentration, pH and bicarbonate addition on biomass and lipid accumulation in the sporulating green alga PW95.” [Algal. Res. 53: 102148]","authors":"L. Corredor ,&nbsp;E.P. Barnhart ,&nbsp;A.E. Parker ,&nbsp;R. Gerlach ,&nbsp;M.W. Fields","doi":"10.1016/j.algal.2025.104036","DOIUrl":"10.1016/j.algal.2025.104036","url":null,"abstract":"","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104036"},"PeriodicalIF":4.6,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consequential life cycle assessment of pretreatment strategies for a microalgae-based wastewater treatment pilot unit in a circular livestock industry bioeconomy","authors":"Anaid López-Sánchez ,&nbsp;Carlos Daniel García-López ,&nbsp;Gary Ossmar Lara-Topete ,&nbsp;Juan Daniel Castanier-Rivas ,&nbsp;Paloma Barajas-Álvarez ,&nbsp;Martín Esteban González-López ,&nbsp;Ana Laura Silva-Gálvez ,&nbsp;Hongying Zhou ,&nbsp;Christopher Q. Lan ,&nbsp;Carlos Eduardo Robles-Rodriguez ,&nbsp;Samir Meramo-Hurtado ,&nbsp;Misael Sebastián Gradilla-Hernández","doi":"10.1016/j.algal.2025.104041","DOIUrl":"10.1016/j.algal.2025.104041","url":null,"abstract":"<div><div>This study presents a Consequential Life Cycle Assessment (CLCA) of a microalgae-based wastewater treatment system for the livestock industry, integrated with a circular bioeconomy approach through biofertilizer production and biogas generation through anaerobic digestion (AD). Three treatment scenarios were evaluated, each employing different combinations of pretreatment technologies, including bioflocculation with chitosan. A scaled-up process model was developed using SuperPro Designer, and the environmental performance was assessed using SimaPro 9.7. Results indicate that scenario with a previous pretreatment using a centrifugation and UV radiation achieved the lowest global warming potential (8129 kg CO₂-eq per ton of dried microalgae), reduced fine particulate matter emissions (38.55 kg PM₂.₅-eq), and required less water consumption (114.29 m³) due to minimal chitosan usage compared to the other scenarios. Sensitivity analysis revealed significant variations in environmental impacts based on microalgae yield and biofertilizer application rates. A 20 % increase in biofertilizer application led to a 27.68 % additional reduction in global warming potential. This research provides compelling evidence for the environmental feasibility and economic viability of large-scale microalgae-based wastewater treatment systems, offering a promising pathway towards sustainable livestock practices and a circular bioeconomy within the agricultural sector.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104041"},"PeriodicalIF":4.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and activity assay in vivo and in vitro of novel antioxidant and anti-aging peptides from C-phycocyanin of Limnospira platensis","authors":"Chen Zhao ,&nbsp;Fuqiang Li ,&nbsp;Shigan Yan ,&nbsp;Liping Zhu ,&nbsp;Shunan Ma ,&nbsp;Tingxin Zhang ,&nbsp;Nan Zhang ,&nbsp;Haiyan Fang ,&nbsp;Guohui Du","doi":"10.1016/j.algal.2025.104042","DOIUrl":"10.1016/j.algal.2025.104042","url":null,"abstract":"<div><div>C-phycocyanin of <em>Limnospira platensis</em> is a safe and nutritious protein with various activities, including antioxidant and anti-aging, indicating it is a potential protein source for preparation of antioxidant and anti-aging peptides. In this study, C-phycocyanin was purified, enzymatically hydrolyzed by seven commercial proteases, and ultrafiltered to obtain ultra-fractions with molecular weights of &lt;3, 3–10, and &gt;10 kDa. Their antioxidant and anti-aging activities were then evaluated <em>in vitro</em> and <em>in vivo</em>. The results demonstrated that the ultrafraction with a molecular weight of &lt;3 kDa from C-PC hydrolysate by Papain (CPCPH-F3) exhibited high antioxidant and anti-aging activities <em>in vitro</em> and in <em>Caenorhabditis elegans</em>. Subsequently, a batch of peptides was identified from the ultrafractions using UPLC-MS/MS, and their toxicity and activities were predicted through bioinformatics analysis. Among these high antioxidant peptides, three novel antioxidant peptides (FPPWVL, FPPWF, and GW) were selected to be synthesized chemically, and their antioxidation and anti-aging activities were assessed <em>in vitro</em> and in <em>Caenorhabditis elegans</em>. Experimental results showed the three novel peptides exhibited significant antioxidant and anti-aging activities both <em>in vitro</em> and <em>in vivo</em>. These findings are beneficial for the development of bio-peptides and high-value utilization of <em>Limnospira platensis</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104042"},"PeriodicalIF":4.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonic assisted enzymatic extraction, structural characterization, and tyrosinase inhibition mechanism of phlorotannins from Sargassum horneri","authors":"Yongqi Tian ,&nbsp;Ding Deng ,&nbsp;Haitao Luo ,&nbsp;Mingfeng He ,&nbsp;Shaoyun Wang","doi":"10.1016/j.algal.2025.104038","DOIUrl":"10.1016/j.algal.2025.104038","url":null,"abstract":"<div><div>Phlorotannins, recognized for their efficacy and safety as natural tyrosinase in<em>hibitors, have posed challenges</em> due to their limited extraction efficiency and the elusive nature of their mechanism of action. Here, we developed an ultrasonic-assisted enzymatic extraction (UAEE) protocol that significantly enhanced phlorotannin recovery from Sargassum horneri, achieving a total phenolic content (TPC) of 21.51 mg PGE/g DW. Subsequent purification using solvent extraction and Sephadex LH-20 yielded high-purity alginate phlorotannins (SHP), exhibiting potent DPPH radical scavenging and tyrosinase inhibitory activities, with IC₅₀ values of 22.71 and 26.90 μg/mL, respectively. Structural elucidation based on NMR and MS data suggested that SHP predominantly comprises fucophloroethols, with a degree of polymerization ranging from 3 to 8. Reciprocal plot analysis indicated that SHP inhibits tyrosinase activity through a competitive inhibition mechanism, while fluorescence spectroscopy revealed a dynamic quenching effect of SHP on tyrosinase. Molecular docking studies further elucidated t<em>hat van der Waals forces and</em> hydrophobic interactions are the primary forces governing the interaction between SHP and tyrosinase. Cellular experiments demonstrated the significant inhibitory effects of SHP on both melanin production and tyrosinase activity in B16-F10 cells. This research presents promising lead compounds for the development of novel tyrosinase inhibitors, while also providing theoretical and methodological foundations for the comprehensive utilization of Sargassum horneri.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104038"},"PeriodicalIF":4.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mild sulfonation process of food grade algae for removal of methyl violet and methylene blue toxic dyes: Statistical modelling and desirability function optimization","authors":"Hasan M. Agha ,&nbsp;Ali H. Jawad ,&nbsp;Lee D. Wilson ,&nbsp;Zeid A. ALOthman","doi":"10.1016/j.algal.2025.104040","DOIUrl":"10.1016/j.algal.2025.104040","url":null,"abstract":"<div><div>In this study, food-grade algae (FGA) underwent a mild sulfonation process via hydrothermal process-assisted activation in 1 M sulfuric acid (H₂SO₄) for 4 h at 100 °C. The sulfonated FGA (S-FGA) was applied to be a potential biosorbent for removal of two structural types of cationic dyes, namely, methyl violet (MV) and methylene blue (MB) from aqueous media. Thus, the physicochemical properties of S-FGA biosorbent were investigated using several analytical methods including XRD, FTIR, SEM-EDX, and pH_pzc. Moreover, the adsorptive performance of S-FGA towards MV and MB dyes was statistically optimized using the Box-Benken design (BBD) with desirability functions. In this process, the key biosorption parameters including S-FGA biosorbent dose (A: 0.02–0.1 g/100 mL), pH of the dye's solution (B: 4–10), and the contact time (C: 10–120 min) were optimized. Thus, the desirability function results indicate the best removal of MV (94.9 %) was achieved using 0.09 g/100 mL of S-FGA dose, pH of 8.2, and contact time of 119 min, while MB removal (74 %) was reached using 0.09 g/100 mL of the S-FGA, pH of 9.5, and 109 min of contact time. The kinetic and isotherm profiles of the biosorption process for MV and MB dyes onto the S-FGA surface followed the pseudo-second order (PSO) kinetic and Freundlich isotherm models, respectively. The estimated biosorption dye capacity (Langmuir model) for MV and MB dyes by S-FGA was 224.6 mg/g and 406.1 mg/g, respectively. The thermodynamic analysis indicates a spontaneous and exothermic-driven biosorption process with decreased randomness at the solid-liquid interface (Δ<em>S°</em> &lt; 0). The biosorption of MV and MB dyes by S-FGA was likely governed by contributions due to hydrogen bonding, Yoshida-H bonding, and electrostatic attractions. Hence, the sulfonation of food algae biomass-based biosorbent shows promising potential for the effective removal of these toxic cationic dyes from contaminated water.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104040"},"PeriodicalIF":4.6,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissolved air flotation of Chlorella sp. using chitosan: influence of algal organic matter and growth phase on coagulant dose","authors":"Michaela Pappa ,&nbsp;Sanjaya Lama ,&nbsp;Irem Demir ,&nbsp;Paula Varas Perez ,&nbsp;Peter Adriaensens ,&nbsp;Wouter Marchal ,&nbsp;Cécile Formosa-Dague ,&nbsp;Dries Vandamme","doi":"10.1016/j.algal.2025.104028","DOIUrl":"10.1016/j.algal.2025.104028","url":null,"abstract":"<div><div>This study investigates the efficient separation of <em>Chlorella</em> sp. microalgae using dissolved air flotation with chitosan as a coagulant. Improving the harvesting process, which significantly contributes to total costs and carbon footprint, could lead to competitive microalgae products and commodities. The exponential phase required 0.20 mg·mg⁻¹ chitosan, which is almost two times more than the chitosan dose of 0.12 mg·mg⁻¹ required for the stationary phase of growth, although the algal biomass concentration had increased from 0.26 ± 0.06 to 0.53 ± 0.01 mg·L⁻¹ and the concentration of algal organic matter from 8 to 32 mg C·L⁻¹ in dissolved organic carbon, respectively. It is also shown, via Microscope Force Spectroscopy, that the cell measured directly from the culture had bound algal organic matter to their surface at pH 8.5, increasing their softness (Young's Modulus = 8.1 ± 10 kPa), roughness (4.2 ± 2.4 nm) and interaction with bubbles (2.5 ± 2.1 nN, adhesion 64.4 %), compared to the washed cells in PBS buffer at pH 7.4 (1474 ± 1053 kPa; 1.3 ± 0.4 nm; 0.8 ± 0.5 nN, adhesion 9.1 %, respectively). Polysaccharides, mainly containing arabinose (29.2 ± 6.9 % and 33.0 ± 1.7 % dw polysaccharide) and galactose (34.2 ± 8.1 % and 17.43 ± 1.3 % dw), while acidic residues of the polysaccharides (6.7 ± 2.1 % and 13.1 ± 5.8 % Area) were also present along with protein (9.1 ± 2.2 % and 0.9 ± 0.2 % dw AOM) in exponential and stationary growth phase, respectively. Nevertheless, the relationship between AOM composition and its underlying structure and functionality is not yet fully understood.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104028"},"PeriodicalIF":4.6,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the contribution of algae-rich microbial communities to dissolved organic matter in a eutrophic lake with distinct rainy and dry seasons","authors":"Han Yan ,&nbsp;Hong-Fa Guo ,&nbsp;Jia-Xun Li ,&nbsp;Zhuo Chen ,&nbsp;Shi-Qi Yin ,&nbsp;Mo-Yu Shen ,&nbsp;Yi Tao ,&nbsp;Yin-Hu Wu ,&nbsp;Hong-Ying Hu","doi":"10.1016/j.algal.2025.104037","DOIUrl":"10.1016/j.algal.2025.104037","url":null,"abstract":"<div><div>Significant elevations of chemical oxygen demand (COD) occurred frequently in numerous eutrophic lakes worldwide, and identifying the source of COD was a huge challenge. Such lakes typically contain large numbers of algae for which the contribution to organic matter pollution has not been adequately clarified. This study focused on a high-altitude eutrophic lake (Caohai Lake) characterized by distinct rainy and dry seasons, in which the features of dissolved organic matter (DOM) and the metabolic characteristics of microbial communities during two periods were deciphered. The results indicated that DOM characteristics at different locations were significantly influenced by water sources. Based on the optical parameters, all of the lake water showed autochthonous DOM origins, and the intensities were even higher during the rainy season. Further molecular structure analyses revealed a consistent predominance of highly unsaturated and phenolic substances in the lake, but an increase of 20.4 % in the abundance of aliphatic compounds at lake center during the rainy season compared to that during the dry season. The microbial community, primarily composed of the algae <em>Microcystis aeruginosa</em>, assimilated and transformed inorganic carbon into organic matter within the lake. The partial least squares path model identified that algal density and microbial diversity were the primary factors influencing DOM characteristics in the lake, whereas the impact of nutrients in the water was relatively minor. The work would provide novel theoretical and technical support for organic risk control and ecological remediation in eutrophic lakes.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104037"},"PeriodicalIF":4.6,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterisation of a native cyanobacteria for a microalgal consortium producing PHAs in agrifood residues","authors":"Sergio Serrano-Blanco ,&nbsp;Marc-Antoine Cimon ,&nbsp;Thi Thanh Ha Pham ,&nbsp;Adam P. Harvey ,&nbsp;Simon Barnabé ,&nbsp;Sharon B. Velasquez-Orta","doi":"10.1016/j.algal.2025.104030","DOIUrl":"10.1016/j.algal.2025.104030","url":null,"abstract":"<div><div>Bioprospecting new native microalgae species can be useful for developing solutions for circular economy approaches in local communities. The microalgae-bacteria consortium VERTECH, developed in the Victoriaville Industrial Park wastewater system (Quebec, Canada) and maintained in laboratory conditions, has proven useful for wastewater treatment and biomass production in recent studies. This study aimed to isolate and characterise a novel cyanobacterium found in the VERTECH consortium. The novel filamentous cyanobacterium presented barrel-shaped single cells with a cell diameter of approximately 2 μm. Phylogenetic analysis of the 16S rRNA gene confirmed that the strain belonged to the genus <em>Leptolyngbya</em>, and it was assigned as <em>Leptolyngbya</em> sp. SB090721. Finally, a new consortium made of the new <em>Leptolyngbya</em> sp. strain and <em>Chlorella vulgaris</em> was prepared, and its ability to use various carbon sources (cranberry residues, quinic acid, malic acid, acetate and citrate) for PHA production was investigated. The highest biomass concentration (1.01 g·L⁻¹) was obtained with quinic acid, followed by cranberry residue (0.97 g·L⁻¹), whereas the highest PHA content was observed in the biomass grown in cranberry residues (31.4% w/w). According to the identification by GC/MS, the main chemical compositions of PHA produced by the consortium were PHB and PHBV. In conclusion, the native <em>Leptolyngbya</em> sp. SB090721, when grown in consortium with <em>C. vulgaris</em> and agrifood residues, can efficiently produce PHA-rich biomass useful for new biomaterials.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104030"},"PeriodicalIF":4.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of a novel extremophile green algae, Chlamydomonas pacifica, and its potential as a biotechnology host","authors":"João Vitor Dutra Molino ,&nbsp;Aaron Oliver ,&nbsp;Harish Sethuram ,&nbsp;Kalisa Kang ,&nbsp;Barbara Saucedo ,&nbsp;Crisandra Jade Diaz ,&nbsp;Abhishek Gupta ,&nbsp;Lee Jong Jen ,&nbsp;Yasin Torres-tiji ,&nbsp;Nora Hidasi ,&nbsp;Amr Badary ,&nbsp;Hunter Jenkins ,&nbsp;Francis J. Fields ,&nbsp;Ryan Simkovsky ,&nbsp;Stephen Mayfield","doi":"10.1016/j.algal.2025.104034","DOIUrl":"10.1016/j.algal.2025.104034","url":null,"abstract":"<div><div>We present the comprehensive characterization of a newly identified microalga, <em>Chlamydomonas pacifica</em>, originally isolated from a soil sample in San Diego, CA, USA. This species showcases remarkable biological versatility, including a broad pH range tolerance (6–11.5), high thermal tolerance (up to 42 °C), and salinity resilience (up to 2 % NaCl). Its amenability to genetic manipulation and sexual reproduction <em>via</em> mating, particularly between the two opposing strains CC-5697 &amp; CC-5699, now publicly available through the Chlamydomonas Resource Center, underscores its potential as a biotechnological chassis. The biological assessment of <em>C. pacifica</em> revealed versatile metabolic capabilities, including diverse nitrogen assimilation capability, motility and phototaxis. Genomic and transcriptomic analyses identified 17,829 genes within a 121 Mb genome, featuring a GC content of 61 %. The codon usage of <em>C. pacifica</em> closely mirrors that of <em>C. reinhardtii</em>, indicating a conserved genetic architecture that supports a trend in codon preference with minor variations. Phylogenetic analyses position <em>C. pacifica</em> within the core-Reinhardtinia clade yet distinct from known Volvocales species. The lipidomic data revealed an abundance of triacylglycerols (TAGs), promising for biofuel applications and lipids for health-related benefits. Our investigation lays the groundwork for exploiting <em>C. pacifica</em> in biotechnological applications, from biofuel generation to synthesizing biodegradable plastics, positioning it as a versatile host for future bioengineering endeavors.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104034"},"PeriodicalIF":4.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}