Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Substrate functionalization by cold plasma treatments as an alternative process to the cultivation of microalgae in biofilm: Application to Botryococcus","authors":"Gabriel Giannini Beillon ,&nbsp;Brigitte Veidl ,&nbsp;Justine Marchand ,&nbsp;Fabienne Poncin-Epaillard ,&nbsp;Benoît Schoefs","doi":"10.1016/j.algal.2024.103728","DOIUrl":"10.1016/j.algal.2024.103728","url":null,"abstract":"<div><div>Microalgae are renowned for their diverse production of molecules, including biofuels. However, biotechnological processes aiming at producing these biomolecules have yet to achieve economic sustainability due to the high costs associated with downstream processing, which can make up to 80 % of the total production costs. Since microalgae immobilized on a flat surface are characterized by a higher productivity and an easier harvesting than bulk culture systems, flat cultures may present better economic viability. Nevertheless, immobilizing filamentous or colonial microalgae on a flat surface is challenging due to their inherent 3D development. In this study, we explored the effectiveness of a plasma-modified polyethylene terephthalate flat surface for improving the immobilization of the green freshwater colonial microalga <em>Botryococcus protuberans</em>, a promising taxon for biofuel production. Plasma treatments were found to alter the wettability and surface energy of polyethylene terephthalate substrates. <em>Botryococcus</em> adhesion was enhanced significantly on O₂ plasma-modified substrates compared to untreated substrates. The adhesion was strong enough to prevent colony development in the water column while allowing the development of a biofilm over one month, with minimal impact on their physiology.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103728"},"PeriodicalIF":4.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae as feed additives in poultry: A review on the health-promoting effects","authors":"Sofie Van Nerom ,&nbsp;Bert Coleman ,&nbsp;Renée De Baets ,&nbsp;Filip Van Immerseel ,&nbsp;Johan Robbens ,&nbsp;Evelyne Delezie","doi":"10.1016/j.algal.2024.103733","DOIUrl":"10.1016/j.algal.2024.103733","url":null,"abstract":"<div><div>This review explores the effects of the inclusion of microalgal biomass in feed on the health of poultry (broilers and laying hens). Microalgae have emerged as a promising feed additive, valued not only for their rich nutritional profile, but also for their bioactive substances. Bioactive compounds, such as phenolics, polyunsaturated fatty acids, oligosaccharides and carotenoids, exhibit antioxidant, anti-inflammatory, antibacterial, and antiviral functions, which hold promise for promoting poultry health. With the ban on prophylactic antibiotic use in feed, microalgal biomass emerges as an innovative feed additive to enhance growth performance and prevent health issues in poultry. This review extensively explains the critical health parameters of poultry, including histology of the intestinal tract, intestinal permeability, immunity, antioxidant status and prebiotic effects on the intestinal microbiome. Furthermore, it offers valuable insights into the inclusion of microalgae in poultry feed, promoting these health parameters. It concludes with suggestions for further research and practical recommendations on how to improve poultry health using microalgal biomass.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103733"},"PeriodicalIF":4.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ambivalent effects of mass cultivation of biodiesel producible green alga Tetraselmis striata on a microbial ecosystem: Evidence from mesocosm experiments","authors":"Sae-Hee Kim ,&nbsp;Jin Ho Kim ,&nbsp;Joo-Hwan Kim ,&nbsp;Pengbin Wang ,&nbsp;Tae Gyu Park ,&nbsp;Sangsuk Park ,&nbsp;Yoon-Ho Kang ,&nbsp;Myung-Soo Han ,&nbsp;Bum Soo Park","doi":"10.1016/j.algal.2024.103735","DOIUrl":"10.1016/j.algal.2024.103735","url":null,"abstract":"<div><div>A 280-ton plant for the mass cultivation of <em>Tetraselmis striata</em> was operated from 2012 to 2019 to produce biodiesel fuel in western Korean coastal waters (Incheon) as a pilot project. This was the first instance globally where a microalgal mass cultivation plant for biodiesel production was implemented in coastal waters, and no prior studies had investigated the impact of microalgal mass cultivation plant on surrounding microecosystems. In this study, bioreactors (1× and 10×) mimicking a large-scale <em>T. striata</em> cultivation plant were installed in semi-permeable a mesocosms (5 tons) to assess their impact on the microbial ecosystem. The results showed that the release of large amounts of dissolve organic carbon (DOC) from the <em>T. striata</em> bioreactors. The 10× pond had a DOC concentration of 21.3 mg/L compared to the control pond of 2.1 mg/L. For the Water Quality Index (WQI), the 1× and 10× bioreactor installed mesocosms improved from Class II (Good) at the beginning of the experiment to Class I (Excellent) via decreasing nutrient levels and increasing of DO levels. However, from a biodiversity perspective, the microbial ecosystem deteriorated, with reductions in the diversity of zooplankton, ciliates, and phytoplankton. The correlation analysis and random forest variable importance measures indicated that the primary factor driving these changes was the alteration of the bacterial community due to elevated DOC levels. These findings indicate that while the mass cultivation of <em>T. striata</em> may improve physicochemical water quality, it has adverse effects on biological environments. Therefore, it is crucial to monitor physical, chemical, and biological factors comprehensively when cultivating microalgae on a large scale in marine environments.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103735"},"PeriodicalIF":4.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing nutrient bioavailability in distillery wastewater through electrochemical oxidation for microalgal growth: Insights on biomass yield, nutrient utilisation, and VFA-assisted carbon capture","authors":"Inigo Johnson ,&nbsp;Chandraraj Krishnan ,&nbsp;Mathava Kumar","doi":"10.1016/j.algal.2024.103734","DOIUrl":"10.1016/j.algal.2024.103734","url":null,"abstract":"<div><div>Two-stage treatment of distillery wastewater (DWW) via electrochemical oxidation (EO) using Ti-RuO₂ anodes (35 cm² area) followed by mixotrophic microalgal treatment was investigated. In the first-stage, EO of DWW has improved the bioavailability of nitrogen and phosphorus at 3.95–5.14 mg/Ah and 0.43–1.02 mg/Ah, respectively, which had strong correlation with current density. EO also reduced ∼30 % TOC, 53 % COD and ∼44 % TN. In the second-stage, the ability of a novel microalgae, <em>Asterarsys quadricellulare</em> to mitigate the toxicity of electrochemically oxidised DWW (EO-DWW) while utilising the nutrients effectively was investigated. The mixotrophic algal growth effectively utilised 85 % phosphate and 91 % nitrate present in EO-DWW at a corresponding growth rate of 0.73 d⁻¹. The algal biomass was found to have ∼15 % carbohydrates, ∼12 % lipids and ∼33 % proteins. Subsequently, a bench-scale bubble column photobioreactor investigation was carried out to understand the carbon dynamics during the growth of <em>Asterarsys quadricellulare</em>. The metabolic uptake of monocarboxylic volatile fatty acids (VFA) and nitrate were found to release OH⁻ ions, which eventually helped in dissolving CO₂ in the reactor through a diffusion-limited process. The total energy spent in bench-scale EO system was 840 kWh (3024 kJ) per L of DWW, and the energy recovery potential of second-stage algal reactor was ∼8.7 %. The microtoxicity experiments with <em>Alivibrio fischeri</em> revealed that two-stage treated DWW was found to be safe for reuse as the microalgal growth has abated the toxicity of EO-DWW.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103734"},"PeriodicalIF":4.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cellulose nanocrystals on the emulsion stability and rheological properties of microalgal Pickering emulsions","authors":"Wonsik Shin ,&nbsp;Joung Sook Hong ,&nbsp;Dae Yeon Kim ,&nbsp;Si Yoon Kim ,&nbsp;Kyu Hyun ,&nbsp;Jun Dong Park ,&nbsp;Kyung Hyun Ahn","doi":"10.1016/j.algal.2024.103731","DOIUrl":"10.1016/j.algal.2024.103731","url":null,"abstract":"<div><div>This study investigates the effect of cellulose nanocrystals (CNCs) to Pickering emulsions prepared with microalgal particles (<em>Spirulina</em> sp. (SPI), <em>Chlorella</em> sp. HS2 (CLO)). The microalgae particles show a weak interfacial localization and Pickering behavior on the O/W emulsion depending on the size (avg. drop size ∼5.39 μm with SPI and 22.15 μm with CLO), resulting in a different stabilization effect. When CNC is additionally mixed with the Pickering emulsions including large microalgae particles (CLO), CNC replaces microalgae particles and localizes at the interface, enhancing strong emulsion stabilization. For the Pickering emulsions including small microalgae (SPI), CNC localizes at the continuous phase, forming a network structure regardless of the concentration. This interfacial localization behavior of CNC against microalgae particles is reflected in the rheological behavior of the Pickering emulsion. Depending on the location of CNC, the emulsions exhibit the two-step yielding behavior, mainly attributed to the CNC network in the continuous phase. The complex role of particles in the emulsion system is more sensitively reflected in the large amplitude oscillatory shear (LAOS) region, characterized using the sequence of physical process (SPP) rheological analysis. The maximum elasticity (E_max) in SPP analysis, which indicates the recovery of the deformed structure, exhibits a significant difference, discriminating structural characteristics of CNC dispersion incorporated with microalgae particles. Emulsion with CLO-CNC has lower E_max than the SPI-CNC case because CNC particles disperse at the interface and the continuous phase. Then the distance between CNC particles is longer, resulting in a weak network structure throughout the emulsion. Due to a weak network of CNC, the emulsion is more vulnerable to coalescence compared to the SPI-CNC system. Therefore, this study suggests that CNC particles added to the Pickering emulsion with microalgae compete to localize at the interface and give coalescence suppression effects to the emulsion. Also, for the Pickering emulsion system composed of multi-particles, rheological analysis including SPP analysis successfully indicates structural characteristics and flow-induced stabilization of Pickering emulsions with multi-particles that microscopic characterization could not detect.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103731"},"PeriodicalIF":4.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational fluid dynamics (CFD) simulation modeling for the cultivation of microalgal monoculture in axenic enclosed bubble column photobioreactor (BCPBR)","authors":"Imran Ahmad ,&nbsp;Shahid Husain ,&nbsp;Lamiaa H.S. Hassan ,&nbsp;Nadeem A. Khan ,&nbsp;Sasan Zahmatkesh ,&nbsp;Bing-Jie Ni","doi":"10.1016/j.algal.2024.103725","DOIUrl":"10.1016/j.algal.2024.103725","url":null,"abstract":"<div><div>Researchers are more concerned with axenic-enclosed PBRs, where there is less or no chance of contamination during the production of biochemical and highly valuable metabolites, and monocultures of microalgae are being grown more frequently. It is a closed, manufactured vessel that aids in the photosynthesis of microalgal cells using artificial light or sunlight as the energy source. In this study, the bubble column PBR (BCPBR) was selected because it possessed some advantages over other PBRs for the growth of <em>Chlorella vulgaris</em>. The BCPBR system prevents contact between the enclosed microalgal cells and the environment, allowing the culturing of microalgae species that are difficult to grow in open pond systems. To compare BCPBR performance quantitatively, the efficient mixing expected in BCPBR, as discussed in the literature, was applied to the CFD model. The experimental results observed during the cultivation of <em>C. vulgaris</em> with restaurant wastewater (RWW) in BCPBR clearly showed better mixing, high growth, and improved treatment efficiency. CFD analysis was conducted on the evolution of bubbles in the BCPBR. The Pressure-Implicit with Splitting of Operators (PISO) pressure correction method is used for velocity and pressure coupling. A geo-reconstruct approach is used to construct the interface, and a second-order upwind calculation technique is used to determine the flow parameters. Therefore, CFD simulation in this study will contribute to the following aspects: (i) the volume fraction contours and velocity contours are going to validate the experimental study as the homogenous mixing favors the growth and productivity, (ii) To study how the size of the nozzle and inlet velocity affect the turbulence generated by bubbles in a BCPBR to identify the optimal nozzle size and velocity for the required turbulence.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103725"},"PeriodicalIF":4.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights into the enhanced growth of cyanobacteria by adaptive laboratory evolution in wastewater environments","authors":"Lang Hu ,&nbsp;Xinyi Fang ,&nbsp;Lelei Wen ,&nbsp;Haixin Zhang ,&nbsp;Baoyu Peng ,&nbsp;Changchun Li","doi":"10.1016/j.algal.2024.103724","DOIUrl":"10.1016/j.algal.2024.103724","url":null,"abstract":"<div><div>The expansion of population leads to an increase in nutrient-rich wastewater, posing a threat to the ecosystem. The cultivation of economically beneficial cyanobacteria consumes amounts of freshwater, exacerbating the depletion of freshwater resources. This study investigates the potential of utilizing adaptive laboratory evolution (ALE) to enhance the growth performance of <em>Synechocystis</em> sp. PCC 6803, a model cyanobacterium, in wastewater. After 374 days of ALE, a strain designated as WW was successfully evolved. When cultivated in wastewater, WW exhibited a specific growth rate of 0.317 per day and achieved a dry weight of 0.693 g/L by the 13th day, outperforming the wild type. WW achieved removal efficiencies of 35.55 % for total nitrogen and 60.95 % for total phosphorus in the wastewater. RNA sequencing and photosynthetic measurements revealed that enhanced photosynthetic capacity in the WW contributes to its superior growth performance. The lipid content in WW was 16.19 %, with a notable increase in the proportion of polyunsaturated fatty acids. The shift in fatty acid composition has a consequential impact on biodiesel index, including oxidation stability and saponification number. This study not only demonstrates the effectiveness of ALE in enhancing the growth of cyanobacteria in wastewater for biofuel production, but also offers significant insights into the molecular mechanisms that drive this improved performance.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103724"},"PeriodicalIF":4.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of optimal culture conditions for marine benthic diatom Halamphora coffeaeformis growth and its biochemical properties","authors":"Ninie Diana Baharuddin ,&nbsp;Norulhuda Mohamed Ramli ,&nbsp;Nurarina Ayuni Ghazali ,&nbsp;Hui Teng Tan ,&nbsp;Yam Sim Khaw ,&nbsp;I. Natrah","doi":"10.1016/j.algal.2024.103720","DOIUrl":"10.1016/j.algal.2024.103720","url":null,"abstract":"<div><div><em>Halamphora coffeaeformis</em> is a benthic diatom that exhibits a high-value biochemical composition and can be harnessed for various applications particularly in aquaculture. Despite its potential, significant challenges persist in the optimization of cultivation parameters and scaling up to mass cultivation level. Thus, this study aimed to identify the most suitable substrate, as well as the optimal light intensity, photoperiod, salinity, and pH for the growth of <em>H. coffeaeformis.</em> The biochemical composition of mass-cultured <em>H. coffeaeformis</em> was also analyzed. The findings revealed that polyvinyl chloride (PVC) substrates substantially outperformed bamboo by yielding significantly higher (<em>p</em> &lt; 0.05) growth performance. The maximum specific growth rate (SGR) of <em>H. coffeaeformis</em> was recorded under the following conditions: the light intensity of 120 μmol m⁻² s⁻¹ with an 18: 6 h light/dark cycle (0.200 day⁻¹), the salinity of 30 ppt (0.161 day⁻¹) and the pH of 9 (0.409 day⁻¹). Under these optimized conditions, the mass culture of <em>H. coffeaeformis</em> at 100 L exhibited the highest ash content (38.47 ± 0.17 % dry weight (DW)), followed by crude protein (26.26 ± 0.56 % DW) containing high-quality amino acids, carbohydrates (20.96 ± 0.81 % DW) and crude lipids (4.46 ± 0.56 % DW). The lipid profile was particularly notable for its high saturated fatty acid (SFA) content and sufficient levels of polyunsaturated fatty acids (PUFA), while maintaining the lowest fiber content (0.07 ± 0.01 % DW). These findings underscore the potential of <em>H. coffeaeformis</em> for large-scale aquaculture applications and highlight the importance of fine-tuning cultivation parameters to maximize its biochemical yields.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103720"},"PeriodicalIF":4.6,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the interventional effects of Chlamydomonas reinhardtii peptides on chronic unpredictable mild stress-induced depressive-like model mice through metabolomics and microbiota","authors":"Ya Mao ,&nbsp;Meiting Liu ,&nbsp;Keying Su ,&nbsp;Jintao Xie ,&nbsp;Wenxia Liu ,&nbsp;Lixia Wu ,&nbsp;Xuewu Zhang","doi":"10.1016/j.algal.2024.103715","DOIUrl":"10.1016/j.algal.2024.103715","url":null,"abstract":"<div><div>Depression is a progressive neurodegenerative disease characterized by high prevalence, high suicide rate and high recurrence rate. In this study, the protein from <em>Chlamydomonas reinhardtii</em> was extracted and neutral protease hydrolysate (NPH) was obtained. Its in vitro MAO-A (Monoamine oxidase A) inhibitory activity and in vivo anti-depressive effects in chronic unpredictable mild stress (CUMS) model mice were investigated. The results demonstrated that NPH can improve depressive behaviour in CUMS model mice by elevating neurotransmitter levels and alleviating hippocampal tissue structure damage. The metabolomic analysis of brain and serum samples showed that their common metabolic pathways associated with anti-depressive effects are mainly alanine, aspartate, and glutamate metabolism, glycerophospholipid metabolism, tryptophan metabolism, and caffeine metabolism. Then, the gut microbiota analysis of feces indicated that 8 species with significant changes were associated with anti-depressive effects. Finally, 5 pairs of highly correlated metabolite-bacterium pairs were identified to modulate depressive behaviours. Taken together, the present data suggests that <em>Chlamydomonas reinhardtii</em>-derived hydrolysate could be used for development of functional foods with potential to improve depression.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103715"},"PeriodicalIF":4.6,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing phlorotannins extraction from Fucus vesiculosus using Box-Behnken design: Unveiling techniques for enhanced antioxidant activity and metabolic enzyme inhibition","authors":"Hazem Golshany ,&nbsp;Aya Kamal ,&nbsp;Qun Yu ,&nbsp;Liuping Fan","doi":"10.1016/j.algal.2024.103723","DOIUrl":"10.1016/j.algal.2024.103723","url":null,"abstract":"<div><div>Phlorotannins, phenolic compounds found in brown macroalgae, exhibit potential bioactive effects. This study evaluated the extraction of phlorotannins from <em>Fucus vesiculosus</em> using conventional and ultrasound-assisted extraction (UAE) methods, focusing on solvent-material ratio, solvent concentration, extraction time, and sonication power for UAE. The experiments, designed using a Box-Behnken design, measured total phlorotannin content (TPhC), DPPH, FRAP, and ABTS. The conventional method, under optimal conditions, produced TPhC, DPPH, FRAP, and ABTS values of 512.25 ± 14.26 mg PGE/100 g, 29.70 ± 1.40 mg TE/100 g, 589.57 ± 15.06 mg TE/100 g, and 332.73 ± 31.03 mg TE/100 g, respectively. The UAE method, under its optimal conditions, yielded higher values: 954.00 ± 33.65 mg PGE/100 g, 146.35 ± 3.30 mg TE/100 g, 1037.61 ± 32.93 mg TE/100 g, and 809.95 ± 46.58 mg TE/100 g. The extracts were tested for inhibitory effects on α-amylase and α-glucosidase, with the purified UAE extract showing greater inhibition of α-amylase (IC₅₀ = 7.06 ± 0.67 μg/mL) compared to acarbose (IC₅₀ = 14.63 ± 0.28 μg/mL). For α-glucosidase, acarbose had higher activity (IC₅₀ = 11.54 ± 0.04 μg/mL) than the UAE purified extract (IC₅₀ = 15.04 ± 0.07 μg/mL). UHPLC-qTOF-MS analysis identified various phlorotannin derivatives, with Tetrafucotetraphlorethol (<em>m</em>/<em>z</em> 993) only in the UAE purified extract. This study concludes that purified phlorotannin extract from UAE can potentially regulate α-amylase and α-glucosidase, enzymes linked to metabolic disorders like diabetes and obesity.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103723"},"PeriodicalIF":4.6,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}