Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Sex-specific physiological-biochemical and multi-omics responses of Sargassum thunbergii to ocean acidification","authors":"Danrui Li ,&nbsp;Yuanyuan Wang ,&nbsp;Yan Zhao ,&nbsp;Xuexi Tang","doi":"10.1016/j.algal.2026.104615","DOIUrl":"10.1016/j.algal.2026.104615","url":null,"abstract":"<div><div>Ocean acidification (OA), driven by increasing atmospheric CO₂ concentrations, poses significant threats to the ecologically important intertidal macroalgae. Multiple previous studies have indicated species-specific responses to OA, the sex-specific physiological-biochemical responses and underlying molecular mechanisms in dioecious macroalgae remain poorly understood. In this study, we investigated the responses of male and female <em>Sargassum thunbergii</em> to acidification treatment (2000 ppm CO₂) by integrating physiological-biochemical, transcriptomic, and metabolomic analyses. Both sexes maintained photosynthetic performance, with increased maximum relative electron transport rates (rETRmax). Males exhibited a growth-oriented strategy, characterized by higher accumulation of storage compounds like triglycerides and up-regulation of genes related to the photosynthesis and biosynthesis pathways. In contrast, females displayed a survival-oriented strategy, with reduced carbon storage, increased soluble protein and phenolic substance contents, and up-regulation of genes related to defense- and stress-response pathways. These findings provided physiological-biochemical and molecular evidence for a growth and defense trade-off between male and female <em>S. thunbergii</em> under acidification treatment. Our study provided the mechanistic insights into the sex-specific responses of marine macroalgae to global climate change and highlighted the importance of accounting for sexual dimorphism in predicting the ecological resilience of intertidal macroalgae populations under future ocean conditions.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104615"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individual and combined effects of three phytohormones on lipid productivity and biodiesel quality in Nannochloropsis gaditana under nitrogen starvation","authors":"Wang Liufu ,&nbsp;Ma Xueyan ,&nbsp;Wen Haibo ,&nbsp;Huang Xuxiong ,&nbsp;Jin Wu ,&nbsp;Chen Wanwen ,&nbsp;Wang Yufeng ,&nbsp;Xu Pao","doi":"10.1016/j.algal.2026.104609","DOIUrl":"10.1016/j.algal.2026.104609","url":null,"abstract":"<div><div>To address the biomass-lipid trade-offs in microalgal biodiesel production, this study investigated biodiesel production using <em>Nannochloropsis gaditana</em> ffrc-1 by optimizing fulvic acid (FA), jasmonic acid (JA) and salicylic acid (SA) under nitrogen starvation, along with mechanistic insights into lipid biosynthesis. Using 20 mg/L FA under nitrogen starvation, the lipid content, biomass productivity and lipid productivity peaked at 30.50%, 0.48 g/L/day and 0.1450 g/L/day. With 20 mg/L JA under nitrogen starvation, the lipid content, biomass productivity and lipid productivity peaked at 31.50%, 0.74 g/L/day and 0.2335 g/L/day. Under nitrogen starvation with 2 mg/L SA, the lipid content, biomass productivity and lipid productivity peaked at 30.20%, 0.84 g/L/day and 0.2540 g/L/day. The combination of 22 mg/L FA, 17 mg/L JA and 2.4 mg/L SA by response surface methodology increased lipid productivity to 0.24 g/L/day under nitrogen starvation. The mRNA expression of lipid synthesis enzymes (acetyl-CoA carboxylase beta and diacylglycerol acyltransferase) was consistent with the trend of lipid accumulation. Moreover, the fatty acid profile and key biodiesel properties of <em>N. gaditana</em> ffrc-1 under nitrogen starvation with the supplementation of these phytohormones complied with international standards with improved cold flow performance. This indicated its promise as a sustainable biodiesel feedstock for cold-weather operations. In summary, this study proves that coordinated FA, JA, and SA application under nitrogen starvation effectively breaks the growth-lipid trade-off in <em>N. gaditana</em>, delivering both high lipid productivity and superior biodiesel cold-flow properties.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104609"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trade-offs between diversity and stability mediated by positive cohesion in sediment microbiomes under algal blooms","authors":"Ruyi Li ,&nbsp;Jianfeng Chen ,&nbsp;Qingyang Rao ,&nbsp;Shangsheng Sun ,&nbsp;Yihan Wang ,&nbsp;Misha Zhong ,&nbsp;Qingchuan Chou ,&nbsp;Meng Zhang ,&nbsp;Haojie Su ,&nbsp;Ping Xie","doi":"10.1016/j.algal.2026.104617","DOIUrl":"10.1016/j.algal.2026.104617","url":null,"abstract":"<div><div>Eutrophication-driven algal blooms represent profound disturbances that restructure aquatic ecosystems. However, the mechanisms by which sediment microbial diversity and species interactions govern community stability during such events remain inadequately resolved. Here, we tracked a natural dinoflagellate bloom in Lake Erhai, a eutrophying plateau lake in southwestern China, to assess pre- and post-bloom changes in sediment microbial communities using 16S rRNA and ITS sequencing. Our analyses revealed that algal blooms significantly reduced sediment microbial α-diversity and decreased community heterogeneity. Concurrently, co-occurrence networks exhibited tightened connectivity, evidenced by shorter average path lengths, increased edge density, and a pronounced rise in positive cohesion. Crucially, we found that sampling sites with higher species diversity have lower compositional stability (quantified as resistance to taxon turnover), while that with strong positive cohesion have higher compositional stability. In contrast, functional stability (metabolic potential) showed no significant relationship with either diversity or cohesion, indicating distinct regulatory mechanisms for compositional versus functional stability. These findings demonstrate a clear trade-off between species diversity and cooperative interactions in shaping compositional stability under bloom-induced stress. This work establishes that positive species cohesion, rather than diversity alone, is a critical mediator of microbial community resistance in sediments facing eutrophication pressures. It advances our mechanistic understanding of stability dynamics in microbiomes and underscores the need to integrate interaction networks when predicting ecosystem responses to anthropogenic disturbances.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104617"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotreatment of table olive processing wastewater and simultaneous biomass production by a mixed culture of Picochlorum costavermella and indigenous microorganisms in laboratory and pilot-scale reactors","authors":"Stefania Patsialou ,&nbsp;Dimitris V. Vayenas ,&nbsp;Athanasia G. Tekerlekopoulou","doi":"10.1016/j.algal.2026.104602","DOIUrl":"10.1016/j.algal.2026.104602","url":null,"abstract":"<div><div>Table olive production generates hypersaline, phenol-rich effluents that pose a significant environmental challenge for their treatment, particularly for small-scale processing units. This study investigated the implementation of the halotolerant microalga <em>Picochlorum costavermella</em> as a single-step method for raw, non-sterilized table olive processing wastewater's (TOPW) biotreatment, coupled with biomass production. Autotrophic growth was first assessed in artificial saline media, followed by mixotrophic cultivation in TOPW. TOPW from the “Kalamon” and “Halkidiki” varieties was diluted with seawater, resulting in initial dissolved Chemical Oxygen Demand (d-COD) concentrations of approximately 500 and 1500 mg/L. In laboratory-scale reactors, biomass concentrations reached 740 mg/L, with removals up to 84% d-COD, 86% nitrate, 94% orthophosphate, and 84% total phenolics. Final d-COD was &lt;120 mg/L, meeting Greek discharge limits. In column photobioreactors, biomass reached 475 mg/L, with 79% d-COD and 96% phenolic removal. The mixed microalgae-indigenous microorganism culture effectively removed organic/inorganic pollutant while producing biomass rich in proteins (33%), lipids (25%), and carbohydrates (11%). Substantial decolorization (up to 83%) was also achieved, and column photobioreactors showed good scale-up potential with enhanced bioproduct accumulation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104602"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperproduction of lutein and protein in a novel yellow mutant of Chlorella sorokiniana via modulation of carbon‑nitrogen metabolism under high-cell-density heterotrophic cultivation","authors":"Yucheng Chen ,&nbsp;Zehao Qiu ,&nbsp;Wen Zhang ,&nbsp;Xinxin Huang ,&nbsp;Ruijuan Ma ,&nbsp;Baobei Wang ,&nbsp;Shih-Hsin Ho ,&nbsp;Jianfeng Chen ,&nbsp;Youping Xie","doi":"10.1016/j.algal.2026.104626","DOIUrl":"10.1016/j.algal.2026.104626","url":null,"abstract":"<div><div><em>Chlorella sorokiniana</em> is capable of high-cell-density heterotrophic cultivation, endowing it with exceptional potential for large-scale, efficient lutein production. However, its industrial application has been hindered by low lutein content and the inability to precisely control carotenoid composition. To address these limitations, a chlorophyll-deficient and lutein-enriched yellow mutant <em>C. sorokiniana</em> MT03 was employed to investigate the effects of carbon–nitrogen (C/N) ratio modulation on lutein and protein production under heterotrophic conditions. Systematic optimization identified a C/N ratio of 16 as optimal, resulting in marked enhancement of both lutein and protein accumulation. Integrated physiological and transcriptomic analyses demonstrated that carbon depletion combined with nitrogen repletion coordinately regulated central carbon metabolism, macromolecular biosynthesis, heme homeostasis, and carotenoid biosynthetic pathways. Notably, key genes involved in fatty acid biosynthesis, starch degradation, amino acid biosynthesis, and carotenoid formation were significantly upregulated, highlighting the tight integration of nitrogen availability with carbon partitioning. Heme accumulation was found to enhance CYP97-mediated lutein synthesis, further linking nitrogen status to carotenogenic flux. A two-stage cultivation strategy, comprising (I) fed-batch growth for high-cell-density biomass production, followed by (II) carbon-depleted and nitrogen-replete conditions to trigger lutein and protein synthesis, was successfully implemented in a 5-L bioreactor. This approach yielded a final biomass concentration of 184.46 g/L, with volumetric titers of 481.63 mg/L lutein and 76.31 g/L protein, surpassing most values reported in the literature. These results demonstrate that rational C/N metabolic regulation effectively redirects carbon flux toward target metabolites, providing a scalable and industrially viable platform for the co-production of lutein and protein in microalgae.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104626"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient cationic dodecenyl succinic anhydride modified starch-based flocculant with a broad salinity tolerance: preparation, microalgae harvesting and flocculation mechanisms","authors":"Liu Yang ,&nbsp;Yaohui You ,&nbsp;Chenming Wang ,&nbsp;Wenhua Yang ,&nbsp;Li Zhao ,&nbsp;Han Xiao ,&nbsp;Jie Sun ,&nbsp;Xiangxiang Zhang","doi":"10.1016/j.algal.2026.104565","DOIUrl":"10.1016/j.algal.2026.104565","url":null,"abstract":"<div><div>The use of flocculation to harvest microalgae from the living environment is efficient and energy-saving; however, it is prone to microalgal biomass contamination and sensitive to the environmental salinity. In the present study, cationic dodecenyl succinic anhydride-modified starch (CDCS) was prepared for harvesting microalgae with various salinities. SEM, XRD, FTIR, ¹H NMR, TG substitution degree, contact angle and zeta potential results confirmed the successful synthesis of CDCS with amphiphilic polyelectrolyte and hydrophobically associating polymer properties. As a comparison, cationic starch (CCS) could only flocculate microalgae at relatively low salinities. In contrast, CDCS could harvest microalgae in 3, 10, 15 and 20 ppt salinity environments at dosages of 3, 10, 25 and 100 mg/L, respectively. Hydrodynamic diameter, viscosity and zeta potential measurements of flocculants at different salinities demonstrated that CDCS could maintain relatively stretched molecular conformations and more cationic charges in high-salinity environments. Analysis of zeta potential, floc morphology and floc size suggested that CDCS could form large three-dimensional mesh flocs with strong anti-shear and reflocculation abilities through charge neutralization, bridging adsorption, hydrophobic forces, netting and sweeping. Additionally, microalgal dissolved/bound extracellular organic matter (DEOM/BEOM) was extracted and flocculated by CCS and CDCS for analysis of the three-dimensional fluorescence, protein and polysaccharide contents. Results showed that CDCS with a broad salinity tolerance tended to interact with BEOM, therefore promoting the formation and sedimentation of microalgal flocs. Finally, cost estimates indicated that CDCS can save 40%–90% of the harvesting costs at different salinities compared to CCS.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104565"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermosynechococcus guangdongensis HY-01 sp. nov., a convenient easily transformable thermophilic chassis for photothermobiology","authors":"Xiangjian Liu ,&nbsp;Chen Li ,&nbsp;Ying Jiang ,&nbsp;Lianghuan Wu ,&nbsp;Dawei You ,&nbsp;Maurycy Daroch","doi":"10.1016/j.algal.2026.104597","DOIUrl":"10.1016/j.algal.2026.104597","url":null,"abstract":"<div><div>This study describes the isolation and characterization of <em>Thermosynechococcus guangdongensis</em> HY-01, a novel thermophilic cyanobacterium developed as a microbial chassis for photothermobiology. The strain was isolated from a top stratum of a microbial mat collected at a geothermal area Tangjiaoqiaotou in Guangdong province of China. Its genome, sequenced using a hybrid approach, is the largest reported in the genus at 2.75 Mbp. The expansion of genome could be attributed to the presence of 180 unique genes, many acquired via horizontal gene transfer. These unique genes are often organised into genomic clusters, four of which are composed exclusively of HY-01-specific genes, including two implicated in exopolysaccharide biosynthesis. This genomic feature may contribute to the strain's ecological niche and physiological robustness. Strain delineation as a new species was performed based on the Botanical Code, encompassing 16S rRNA phylogeny, GTDB, Average Nucleotide and Amino Acid Identities together with morphological and habitat data. The polyphasic approach confirmed its status as a new species within the family. Morphologically and physiologically, HY-01 exhibited typical <em>Thermosynechococcus</em> characteristics. Growth profiling demonstrated robust performance across 37–57 °C, with peak at 52 °C and broad optimum between 47 and 57 °C. Critically, a simplified genetic transformation system based on electroporation was successfully established, achieving fully segregated transgenic lines within a fortnight, as validated by diagnostic PCR and protein immunoblotting of His-tagged photosystem I protein psaJ. We conclude that <em>Thermosynechococcus guangdongensis</em> HY-01 represents a genetically tractable and thermotolerant new species, expanding the portfolio of model organisms for studying thermostable photosynthesis and for developing high-temperature biotechnological applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104597"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coelastrella sp. biofilm integrated on agave-derived carbon for enhanced anodic performance in biophotovoltaic energy harvesting","authors":"Patrick U. Okoye ,&nbsp;Itzel Montes-Roman ,&nbsp;Rosa Angelica Guillen-Garcés ,&nbsp;Francisco Vera-Lopez Portillo ,&nbsp;Diego Lobato-Peralta ,&nbsp;Dulce M. Arias-Lizarraga","doi":"10.1016/j.algal.2026.104594","DOIUrl":"10.1016/j.algal.2026.104594","url":null,"abstract":"<div><div>The development of sustainable electrode materials that allows sufficient accumulation of redox species is key to advancing biophotovoltaic (BPV) technologies. In this study, activated carbon derived from agave fiber was evaluated as an anodic electrode material for BPV devices. Activated carbon samples were synthesized at 600, 700, and 800 °C using different agave biomass-to-KOH impregnation ratios from 1:1 to 1:3. The resulting carbons were comprehensively characterized to analyze their morphology and composition, and to establish structure–performance relationships. Electrochemical evaluation using <em>Coelastrella</em> sp. biofilms revealed that the electrode produced at 700 °C with a 1:2 ratio delivered the best performance, achieving a specific capacity of 3.50 mAh/g. Both impregnation ratio and activation temperature influenced biofilm accumulation, while a balanced <em>meso</em>–micropore structure favored electron transfer from the bulk to the anode surface, ensuring sufficient interaction with photosynthetic microorganisms of <em>Coelastrella</em> sp. The maximum power density achieved was 1.3 mW/m², with an open-circuit voltage of ∼140 mV at the lowest current density (∼6 mA/m²), and a short-circuit current density of ∼24 mA/m². These findings demonstrate the potential of <em>Coelastrella</em> sp. biofilms to enhance the functionality of electrochemical materials by improving electron transfer kinetics, thereby contributing to the development of clean technologies that harness solar energy.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104594"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geography and seasonality as factors affecting lipid classes and anti-inflammatory activity of Rugulopteryx okamurae, an invasive seaweed in Southwest Europe","authors":"A.M. García Cervantes ,&nbsp;M. Carmona-Fernández ,&nbsp;A. Gomes-Bispo ,&nbsp;C. Cardoso ,&nbsp;C. Afonso ,&nbsp;J.L. Guil-Guerrero ,&nbsp;N.M. Bandarra","doi":"10.1016/j.algal.2026.104618","DOIUrl":"10.1016/j.algal.2026.104618","url":null,"abstract":"<div><div><em>Rugulopteryx okamurae</em> is an invasive brown seaweed originating in the Pacific Ocean that has spread to Southwestern European shores in the last decade, reaching high densities and causing large ecological and economic impacts. The lipid classes and anti-inflammatory activity of this seaweed species was investigated, comprising an analysis of different geographical locations in the Mediterranean Sea and Atlantic Ocean as well as winter and summer as growing seasons. The fatty acid (FA) composition of the biomass, main lipid classes, and cyclooxygenase-2 (COX-2) inhibition were analysed. There is evidence supporting a possible class-level lipid distribution pattern specific to <em>R. okamurae</em>, differentiating it from other brown seaweeds. The FA profile revealed prevalence of palmitic acid and saturated FA (SFA) in all lipid classes. Lipid class distribution in <em>R. okamurae</em> was characterized by a majority of polar lipids, with non-polar lipids making up to 40% of the total lipid content. Within polar lipids, glycolipids were the main class and monogalactosyldiacylglycerols (MGDG) had a share ranging from 45 to 58% of the total lipids. The SFA content ranged between 30.8 ± 1.3% (of total FA) in Atlantic (Algarve) <em>R. okamurae</em> MGDG and 82.5 ± 2.6% in Atlantic <em>R. okamurae</em> phospholipids. The FA profiles of <em>R. okamurae</em> from the two studied locations were quite similar, differing in palmitic acid content in triacylglycerols (TAG), oleic acid in MGDG, PUFA in digalactosyl diacylglycerols (DGDG), and n-3/n-6 ratio in MGDG. Regarding this latter parameter, the highest n-3/n-6 ratio was in Algarve MGDG samples, 1.8 ± 0.1. The preliminary screening of anti-inflammatory activity by the COX-2 assay showed higher levels of this parameter in summer <em>R. okamurae</em> (76.3 and 78.5% COX-2 inhibition). Therefore, <em>R. okamurae</em> exhibits distinctive traits and there is a potential valorization by component isolation (e.g. MGDG) and further refining of biologically active fractions (e.g. anti-inflammatory ethanol extracts).</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104618"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking lipid droplet dynamics using BODIPY C12 in Phaeodactylum tricornutum","authors":"Ayushi Dalmia,&nbsp;Inna Khozin-Goldberg","doi":"10.1016/j.algal.2026.104622","DOIUrl":"10.1016/j.algal.2026.104622","url":null,"abstract":"<div><div>Oleaginous microalgae are central to fundamental research on microalgae and biotechnology for single-cell oil production. Although many tools to manipulate physiological and molecular mechanisms have been developed for microalgae, cellular biology tools that enable visualization of lipid droplet (LD) dynamics in vivo are lacking. In this study, we demonstrate the use of the fluorescent fatty acid conjugate BODIPY C12 (BP C12) to study LD dynamics in two ecotypes of the model diatom <em>Phaeodactylum tricornutum</em> Pt1 and Pt4, as well as fatty acid trafficking in vivo, through real-time visualization of LD formation and breakdown by epifluorescence microscopy and FACS. Cells supplied with BP C12 under nutrient-replete conditions exhibited incorporation of BP C12 signal inside the cells in tiny vesicles. Under nitrogen starvation, the BP C12 signal was localized to the preformed LDs. During recovery from nitrogen starvation, we observed the breakdown of the LDs harbouring the BP C12 signal. Thin-layer chromatography showed that most BP C12 remained unesterified, but the signal was found in both polar and neutral lipids, suggesting its suitability for lipid-metabolic studies in <em>P. tricornutum</em>. Use of BP C12 also elucidated substrate utilization in the two ecotypes. These findings pave the way for the use of BP C12 in various assays with <em>P. tricornutum</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104622"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}