Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Expanding the Egbenema horizon: Novel species discovery in India and first complete genome assembly of the genus","authors":"Jayesh M. Bhagat ,&nbsp;Ajinkya A. Khilari ,&nbsp;Dhanasekaran Shanmugam ,&nbsp;Meena Patankar","doi":"10.1016/j.algal.2025.104256","DOIUrl":"10.1016/j.algal.2025.104256","url":null,"abstract":"<div><div>The recognition of <em>Egbenema bharatensis</em>, a novel species of cyanobacteria from the Northern Western Ghats, extends the ecological and phylogenetic understanding of the genus <em>Egbenema</em> and highlights terrestrial microbial diversity of the region. Morphological characterization revealed resemblance with other <em>Egbenema</em> species but was inconclusive for definitive taxonomic classification. Preliminary phylogenetic analysis based on 16S rRNA gene sequences indicated affiliation with the genus but showed less than 98 % similarity to known species, pointing toward taxonomic distinctiveness. Analysis of the 16S–ITS region further supported this differentiation, revealing unique secondary structural features. Based on these findings, we propose the isolate as a novel species within <em>Egbenema</em>. Whole-genome sequencing provided full resolution and functional genome annotation revealed nitrogen fixation, cobalamin biosynthesis, and stress tolerance pathways, suggesting its metabolic versatility and potential biotechnological applications. The presence of multiple toxin-antitoxin systems also reflects its adaptability to environmental fluctuation. This study also presents the first complete genome of the genus <em>Egbenema</em>, filling a significant gap in cyanobacterial genomic resources. These findings encourage integrative taxonomic practices and affirm the significance of exploring biodiversity hotspots for uncovering cryptic microbial diversity with ecological and industrial potential.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104256"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144828239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arthrospira platensis polysaccharide alleviates inflammation induced by PRV in RAW264.7 cells and mice via the regulation of LncRNA Dhx35/mmu-miR-185-3p/IL-17RA","authors":"Shouli Yi ,&nbsp;Xiaodong Xie ,&nbsp;Xiaoli Yu ,&nbsp;Min Tang ,&nbsp;Duong Thi Toan ,&nbsp;Meiling Yu ,&nbsp;Mixia Cao ,&nbsp;Tingjun Hu","doi":"10.1016/j.algal.2025.104259","DOIUrl":"10.1016/j.algal.2025.104259","url":null,"abstract":"<div><div>Pseudorabies virus (PRV) poses a great danger to pig farming and human public health safety. Previous studies have shown that the first component of <em>Arthrospira platensis</em> polysaccharide (PAP-1) may alleviate the inflammatory response of RAW264.7 cells induced by PRV infection through the IL-17RA signaling pathway and its circRNA-miRNA-mRNA network, but the specific mechanism is not clear. In this study, we screened the LncRNA sequences related to IL-17RA signaling pathway by transcriptomics data. The mechanism was verified by gene interference or overexpression and dual luciferase experiments to investigate the regulatory mechanism of PAP-1 on IL-17RA inflammatory signaling pathway in PRV infection. In vitro studies showed that PAP-1 significantly reduced the PRV-induced elevated expression of IL-17RA signaling pathway inflammatory factors such as IL-17RA, IL-1β, CXCL10, and iNOS in RAW264.7 cells (<em>P</em> &lt; 0.05). On this basis, the key regulatory factor LncRNA Dhx35 of IL-17 RA signaling pathway was screened by whole transcriptome sequencing analysis. It was found that PAP-1 could reduce the transcriptional activity of IL-17RA by down-regulating PRV-induced LncRNA Dhx35 as a competing endogenous RNA to bind to mmu-miR-185-3p, which decreased the inflammatory response. In vivo experiments revealed that PAP-1 down-regulated the expression of LncRNA Dhx35, up-regulated the expression of mmu-miR-185-3p, and significantly down-regulated the expression of IL-17RA, IL-1β, CXCL10, and iNOS in lung tissues of PRV-infected mice (<em>P</em> &lt; 0.05). Taken together, our data suggest that PAP-1 reduces PRV infection-induced inflammatory responses in RAW264.7 cells and mouse lung tissues by regulating LncRNA Dhx35/mmu-miR-185-3p/IL-17RA.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104259"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation, structure and activity determinations of novel immunomodulatory peptides from Limnospira platensis","authors":"Chen Zhao ,&nbsp;Tingxin Zhang ,&nbsp;Shunan Ma,&nbsp;Rui Deng,&nbsp;Liping Zhu,&nbsp;Shigan Yan","doi":"10.1016/j.algal.2025.104262","DOIUrl":"10.1016/j.algal.2025.104262","url":null,"abstract":"<div><div>Immunomodulatory peptides derived from food proteins have attracted worldwide attention. In this study, phycocyanin-derived peptides from <em>Limnospira platensis</em> were prepared using enzymatic hydrolysis with commercial proteases and ultrafiltration, and investigated their immunomodulatory activity on RAW264.7 cells. The results showed that papain hydrolysate ultrafiltration with molecular weight of &lt;3 kDa (named PP) could promote the cell viability of RAW264.7. Subsequently, a series of peptides was determined from PP using UPLC-MS/MS, and their toxicity and activities were predicted through PeptideRanker analysis. Six novel peptides with high potential immunomodulatory activity, FGRFR, SPSWY, YNKFPY, GYYLRM, MFDAF, and MAACLR, were screened to be synthesized chemically and assessed their immunomodulatory activities on RAW264.7. Experimental results demonstrated these six novel synthetic peptides could increase immune function and cytokine secretion of RAW264.7 cells. Meanwhile, they also showed stability after simulated gastrointestinal digestion and treatment at different temperatures and pH levels. Moreover, molecular-docking assays indicated their immunomodulatory activities were related to the interaction between peptides and TLRs receptors. These phycocyanin hydrolysate and novel immunomodulatory peptides from <em>Limnospira platensis</em> have potential applications in functional foods and medicine.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104262"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the efficiency and scalability of using algae as a biomass feedstock for biofuel production","authors":"Niranjana Karukayil Gopalakrishnan ,&nbsp;Balamuralikrishnan Balasubramanian ,&nbsp;Arun Meyyazhagan ,&nbsp;Aditi Chaudhary ,&nbsp;Vino Palani ,&nbsp;Hesam Kamyab ,&nbsp;Manikantan Pappuswamy","doi":"10.1016/j.algal.2025.104251","DOIUrl":"10.1016/j.algal.2025.104251","url":null,"abstract":"<div><div>Sustainability is paramount to preserving essential resources for future generations. The widespread use of fossil fuels generates significant pollution, severely impacting both terrestrial and aquatic ecosystems through phenomena such as acid rain. Despite their rapid growth, high photosynthetic efficiency, and ability to thrive in a variety of conditions, algae have become a viable alternative biomass feedstock for biofuel production. This review explores the efficiency and scalability of algae-based biofuels, focusing on key factors such as biomass yield, lipid content, and conversion technologies. Algae have a higher lipid yield compared to traditional biofuel feedstocks such as corn or soybeans, making them an attractive option for large-scale fuel production. However, several obstacles hinder the widespread adoption of algae-based biofuels, including high production costs, energy-intensive cultivation, and water consumption. This paper also examines the efficiency and suitability of various cultivation technologies, including open ponds and photobioreactors, for large-scale production. Algal biofuel production could become more economically viable and environmentally sustainable through the integration of carbon capture technology and wastewater treatment. Advances in genetic engineering and metabolic optimization are further increasing lipid productivity, offering promising prospects for large-scale applications. This review additionally provides an analysis of genetic engineering techniques aimed at increasing biofuel yields. The study emphasizes the potential of algae-based biofuels to serve as environmentally friendly alternatives to traditional fossil fuels, highlighting these innovative approaches. While the evaluation acknowledges that algae-based biofuels can reduce dependency on fossil fuels and help mitigate climate change, it also notes that further research and development are necessary to overcome current financial and technological challenges. This review explores the recent advancements in algae cultivation, harvesting techniques, and biofuel extraction processes. Its goal is to present a comprehensive understanding of the current state of algae as a sustainable and effective feedstock for biofuel production, along with future prospects.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104251"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen to biomass, chlorophyll and carotenoids transfer functions for microalgae Scenedesmus sp.","authors":"Juan Cristóbal García-López ,&nbsp;Gema L. López-Lizárraga ,&nbsp;Juan Cristóbal García-Cañedo","doi":"10.1016/j.algal.2025.104257","DOIUrl":"10.1016/j.algal.2025.104257","url":null,"abstract":"<div><div>Mayor nutrients in autotrophic growth are solar energy, carbon dioxide, nitrogen as nitrates or as ammonia, and other macro and micronutrients. Some microalgae products with a high commercial value require a certain concentration of nitrogen to be reached or maintained in the medium during cultivation. For example, <em>Haematoccocus pluvialis</em> is used to produce astaxanthin (a secondary metabolite) and requires first a growth stage with high nitrogen concentrations, and then an induction stage with nitrogen deprivation in the medium. In the case of <em>Scenedesmus</em> used to produce lutein (primary metabolite), it is necessary to maintain nitrogen concentrations in the medium above a certain concentration in order to promote production and accumulation of lutein in the biomass. Nitrogen concentration for lutein production is usually determined by experimentation to be suitable for the species being used and culture conditions. In several species of microalgae biomass maximum concentration is also influenced by nitrogen concentrations in the medium. In the present article we present transfer functions of nitrogen cultivation associated with biomass and pigment production under autotrophic conditions in batch and fed batch culture modes. Transfer-function representations are presented in the document and compared to experimental data and discussed. Transfer function complexity increased with increased nitrogen level in both culture modes. Control theory can be used in microalgae cultures to model behavior in response to nutrients such as nitrogen. With the proper data and transfer function, nitrogen modeling and control may be achieved in microalgae cultivations for biomass and high value molecules production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104257"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144828725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticoagulant potential of sulfated galactofucan Turbinaria decurrens (Bory de Saint-Vincent, 1828) in human umbilical vein endothelial cells: A marine-derived approach to thrombotic disorder management","authors":"Ans Mariya George ,&nbsp;Kajal Chakraborty ,&nbsp;P. Sreeshma ,&nbsp;Ashwin Ashok Pai ,&nbsp;Silpa Kunnappilly Paulose","doi":"10.1016/j.algal.2025.104258","DOIUrl":"10.1016/j.algal.2025.104258","url":null,"abstract":"<div><div>Sulfated galactofucans from the Sargassaceae family are garnering increasing attention for their therapeutic potential, especially in the management of thrombotic disorders, which are major contributors to disease and mortality. While conventional anticoagulants are effective, their use is constrained by bleeding risks and adverse effects, prompting the search for safer alternatives. This study explores the anticoagulant potential of TDP-3, a sulfated galactofucan isolated from <em>Turbinaria decurrens</em>, with the proposed structure [→4)-3-O-SO₃⁻-<em>α</em>-Fuc<em>p</em>-(1 → 4)-<em>β</em>-Gal<em>p</em>-(1→], in human umbilical vein endothelial cells (HUVECs). TDP-3 (25 <em>μ</em>g/mL) significantly prolonged activated partial thromboplastin time (aPTT: 112.45 s) and prothrombin time (PT: 93.80 s), indicating its ability to interfere with both intrinsic as well as extrinsic coagulation pathways. TDP-3 effectively reduced thrombin production to 49 %, with a significant decrease of Factor Xa (FXa) expression (6.28-fold) and phospho-MARCKS (p-MARCKS) expression (84.1 %). Furthermore, TDP-3 inhibited thrombin-catalyzed fibrin polymerization by 79.19 % and reduced intracellular calcium mobilization by 33.65 %. The compound also demonstrated notable antiplatelet activity, reducing ADP-induced platelet aggregation to 82.9 %. TDP-3 consists mainly of <em>α-</em>(1 → 4)-linked fucose monomers connected to <em>β</em>-galactose, with sulfate groups at the 3-<em>O</em> position, forming a repeating chain. The superior anticoagulant potential of TDP-3 can be attributed to its electronegative sulfate and hydroxyl groups, which modulate physicochemical characteristics, including molecular polarizability and topological polar surface area favorably, thereby enhancing hydrogen bonding interactions with key receptors in coagulation pathways. These findings position TDP-3 as a promising natural anticoagulant with potential therapeutic applications for managing thrombotic disorders. Its potential as a functional food further contributes to the expanding field of marine-derived anticoagulants.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104258"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144828969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary study on life history of Erythrotrichia bohanensis (Erythropeltales, Rhodophyta)","authors":"Yue Chu ,&nbsp;Lanping Ding ,&nbsp;Yanguang Zhong ,&nbsp;Zhimiao Wang ,&nbsp;Yuan Gao ,&nbsp;Meina Chen ,&nbsp;Chang Sun ,&nbsp;Bingxin Huang","doi":"10.1016/j.algal.2025.104279","DOIUrl":"10.1016/j.algal.2025.104279","url":null,"abstract":"<div><div><em>Erythrotrichia bohanensis</em> Huang, Chu et Ding is a recently reported new species, with the type specimen originating from Qinhuangdao, China. Being a lower filamentous red alga, it lives an epiphytic life. In this study, <em>E. bohanensis</em> was isolated from red and brown macroalgae collected in Qinhuangdao, Hebei, in spring 2023. They were cultured under laboratory conditions, and their life cycle was an alternation of sporophyte and gametophyte generations. Thalli are monoecious, with mature gametophytes producing carpogonia and spermatangia. Spermatangia release spermatia through “post-maturation” (maturation after detachment from the thallus) and “over-maturation” (maturation within the thallus). Fertilization occurs between sperms and ova outside the thallus or on the thallus surface, and the zygote germinates to form the sporophyte. Mature sporophytes release monospores, which germinate into gametophytes outside the thallus. This study first reports the life cycle of the new species <em>E. bohanensis</em>, supplementing the sexual reproductive structures and early life history pathways of the genus <em>Erythrotrichia</em>. It also enriches understanding of the diverse reproductive strategies and life history processes that characterize the adaptive capabilities of lower marine red algae to their environments.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104279"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of Chlorella polysaccharide using natural deep eutectic solvent","authors":"Bingfeng Zhou ,&nbsp;Jiaming Jiang ,&nbsp;Yu Huo ,&nbsp;Juying Lin ,&nbsp;Huiqing Zhang ,&nbsp;Changhua Shang","doi":"10.1016/j.algal.2025.104260","DOIUrl":"10.1016/j.algal.2025.104260","url":null,"abstract":"<div><div>The conventional extraction methods of polysaccharides have some disadvantages such as polysaccharide degradation, the changes in structure and biological activity of polysaccharides due to too long time and too high temperature. Compared with the conventional extraction methods, the green natural deep eutectic solvent (NADES) has a promising application in various industries. Although NADES has been widely used to extract many substances from algae including polysaccharides, polysaccharide extraction from <em>Chlorella</em> was rare. In this study, <em>Chlorella pyrenoidosa</em> polysaccharides were extracted by ultrasound-assisted NADESs. Among 10 NADESs, distilled water and 50 % ethanol, DES 7 with the highest extraction efficiency of <em>Chlorella</em> polysaccharides was selected for further research. Single factor experiment and Box–Behnken design (BBD) optimization for extraction conditions of DES 7 were performed to obtain the best extraction conditions and extraction efficiency. To further investigate antioxidant activities of <em>Chlorella</em> polysaccharide (CHP), DPPH/ABTS radical scavenging activities and iron reducing power of polysaccharide CHP were determined. The most appropriate NADES was DES 7 (choline chloride:ethanol) with extraction efficiency of 3.09 %. After the experimental optimization with BBD of response surface methodology, the optimal extraction conditions for DES 7 were solid-to-liquid ratio of 1:30, molar ratio of 1:5.878 and water content of 20 %. Under the ideal extraction conditions, the real extraction efficiency of polysaccharides CHP was 4.256 % (more than the initial 3.09 %), which was obviously higher than that of polysaccharides from <em>Chlorella vulgaris</em> in previous studies (5.32 mg/g DW and 17.35 mg/g DW). Furthermore, <em>Chlorella pyrenoidosa</em> powder was prepared by spray drying with lower production cost compared with other drying techniques like freeze-drying. In addition, CHP exhibited strong DPPH and ABTS scavenging activities (38.19 % and 86.32 %) and iron reducing power (OD₇₀₀ = 1.56), respectively. In this study, a novel and green method for extracting <em>Chlorella</em> polysaccharide CHP was proposed, which provided the good basis for the application of <em>Chlorella</em> polysaccharides in food, cosmetic and pharmaceutical industries.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104260"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144828724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allelopathic inhibition of Oocystis borgei on Microcystis aeruginosa: Mechanisms and identification of the predominant allelochemicals","authors":"Liangmou Wang ,&nbsp;Ya He ,&nbsp;Hui Yang ,&nbsp;Qian Zhang ,&nbsp;Dapeng Li ,&nbsp;Li Li","doi":"10.1016/j.algal.2025.104243","DOIUrl":"10.1016/j.algal.2025.104243","url":null,"abstract":"<div><div>The increasing frequency of blue-green algae blooms has exerted significant detrimental impacts on aquatic ecosystem health. This study investigated the allelopathic effects of <em>Oocystis borgei</em> filtrate (at concentrations of 0 %, 25 %, 50 %, 75 %, and 100 %) on <em>Microcystis aeruginosa</em> and identified key inhibitory compounds. Our results demonstrate that <em>O. borgei</em> filtrate significantly inhibited <em>M. aeruginosa</em> growth, as indicated by reduced cell density, Chl-a content, and photosynthetic efficiency metrics (F_v/F_m and Y(II)). Mechanistic analyses demonstrated that the filtrate disrupted cell membrane integrity, thereby inducing intracellular toxin release and altering cellular composition through a concomitant decrease in protein-like substances and increase in humic-like substances. GC–MS analysis identified multiple bioactive allelochemicals in the <em>O. borgei</em> filtrate, including phenolic compounds (3,5-di-tert-butylphenol, 2,4-di-tert-butylphenol), phenol derivatives (2,2′-methylenebis(4-methyl-6-tert-butylphenol)), and esters/phthalates (erucamide, tributyl citrate, dibutyl phthalate, di(2-ethylhexyl) phthalate, and diisooctyl phthalate). Notably, 3,5-di-tert-butylphenol exhibited the strongest inhibitory effect (7-day EC₅₀ = 3.61 mg/L, IQ =1.83). These findings underscore the capacity of <em>O. borgei</em>-derived allelochemicals to suppress harmful <em>M. aeruginosa</em> growth, offering a species-specific allelopathic strategy to precision-target harmful <em>Microcystis blooms</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104243"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-engineered thermotolerant Saccharomyces cerevisiae JAYET for enhanced 3G-ethanol production from Chlorella vulgaris biomass","authors":"Maria Augusta de Carvalho Silvello ,&nbsp;Gleidson Silva Teixeira ,&nbsp;Danilo Bueno ,&nbsp;Rosana Goldbeck","doi":"10.1016/j.algal.2025.104275","DOIUrl":"10.1016/j.algal.2025.104275","url":null,"abstract":"<div><div>The production of third-generation (3G) ethanol from microalgae-derived carbohydrates requires a cost-effective hydrolysis strategy and a robust fermenting microorganism. In this study, the <em>amyA</em> gene encoding α-amylase from <em>Aspergillus tubingensis</em> was integrated into the genome of the thermotolerant <em>Saccharomyces cerevisiae</em> JAYET strain using CRISPR/Cas9. JAYET was previously derived from the industrial PE-2 strain and engineered for cellulase production. The CRISPR/Cas9 system enabled efficient genome editing and construction of a synthetic yeast strain, named JAYCA. The performance of the engineered strain was evaluated under different fermentation strategies (SHF, SSF, pSSF), temperatures (30 °C and 40 °C), and compared with parental strains. JAYCA showed strong growth on starch agar, confirming successful expression of extracellular α-amylase. During fermentation of carbohydrate-rich <em>Chlorella vulgaris</em> biomass, JAYCA achieved 92.1 % of the theoretical ethanol yield using Separate Hydrolysis and Fermentation (SHF) at 30 °C. The highest ethanol concentration (16.41 g·L⁻¹) was obtained under Simultaneous Saccharification and Fermentation (SSF), combining α-amylase and amyloglucosidase activity. These results demonstrate that the engineered yeast effectively converted microalgal carbohydrates into ethanol, achieving high 3G ethanol titers through an eco-friendly Consolidated Bioprocessing (CBP) strategy based on enzymatic hydrolysis and renewable biomass. This is the first report of applying the engineered JAYCA strain in a microalgae-based CBP strategy for 3G ethanol production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104275"},"PeriodicalIF":4.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144885837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}