Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Discerning promotion mechanisms of fungi Clonostachys rosea on growth of freshwater microalga Chlorella sp. by non-contact culture","authors":"Juan Liu ,&nbsp;Shiqing Sun ,&nbsp;Kangyu Chen ,&nbsp;Shumei Gao ,&nbsp;Dixing Chen ,&nbsp;Yuejin Zhang ,&nbsp;Changwei Hu","doi":"10.1016/j.algal.2025.103967","DOIUrl":"10.1016/j.algal.2025.103967","url":null,"abstract":"<div><div>Fungi-microalgae consortia have great potential in microalgae harvesting and wastewater treatment. However, the symbiotic mechanisms underlying diffusible signaling metabolite production by fungi and microalgae remain unclear. Here, the positive impacts of <em>Clonostachys rosea</em> on <em>Chlorella</em> sp. were investigated using metabolomics and transcriptomics during a 5-day non-contact co-culture experiment. Co-cultured <em>Chlorella</em> sp. had a significantly higher growth rate and better photosynthetic performance than its mono-culture equivalent. The metabolites played important roles in the up-regulation of energy and amino acid metabolism processes in algal cells, whilst transcriptomics showed the up-regulation of genes involved in photosynthesis and signaling molecules. The biosynthesis of tryptophan and indole-3-acetic acid (IAA) was up-regulated in both algal cells and fungi, indicating that IAA is an important diffusible signaling molecule for algal growth regulation. This work provides novel insights into the signaling molecule interactions between fungi and microalgae in fungi-microalgae consortia.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103967"},"PeriodicalIF":4.6,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetradesmus obliquus organic extracts with antibacterial and antileishmanial activities","authors":"Millena Patrício do Nascimento Ferreira ,&nbsp;Alexsandra Frazão de Andrade ,&nbsp;Jorge Vinícius Fernandes Lima Cavalcanti ,&nbsp;João Carlos Monteiro de Carvalho ,&nbsp;Marcelo Chuei Matsudo ,&nbsp;Ana Lúcia Figueiredo Porto ,&nbsp;Milena de Paiva-Cavalcanti ,&nbsp;Valéria Rêgo Alves Pereira ,&nbsp;Daniela de Araújo Viana-Marques ,&nbsp;Silvana de Fátima Ferreira da Silva Caires ,&nbsp;Raquel Pedrosa Bezerra","doi":"10.1016/j.algal.2025.103970","DOIUrl":"10.1016/j.algal.2025.103970","url":null,"abstract":"<div><div>Current treatments for leishmaniasis often result in side effects and bacterial infections that inhibit the healing of skin lesions, inducing a need to find new natural therapies. Microalgal bioactives can offer a potential alternative for treating leishmaniasis. This work aimed to evaluate the extraction yield from <em>Tetradesmus obliquus</em> microalgae using three different solvents (hexane, ethyl acetate, and ethanol), as well as to determine fatty acid profile, antibacterial and antileishmanial activities (<em>Leishmania braziliensis</em> promastigote), and cytotoxicity on human peripheral blood mononuclear cells (PBMC). In the first extraction, hexane achieved the highest yield (3.50 %) while ethanol was the most efficient solvent in the second extraction (4.47 %). The predominant fatty acids were palmitic acid (18.7 % to 20.6 %) and α-linolenic acid (15.4 % to 19.9 %). The ethanolic extract exhibited activity against <em>S. aureus</em>, <em>E. coli</em>, and <em>K. pneumoniae</em> as well as antileishmanial activity against <em>L. braziliensis</em> (IC₅₀ = 75 μg mL⁻¹), outperforming the reference drug meglumine antimoniate (Sb^V; IC₅₀ of 119.76 μg mL⁻¹). The extract showed no cytotoxicity to PBMCs at the tested concentrations (CC₅₀ &gt; 250 μg mL⁻¹) and exhibited a selectivity index (SI &gt;3.33) comparable to Sb^V (SI &gt; 3.44). In conclusion, the ethanolic extract of <em>T. obliquus</em> emerges as a promising candidate for novel therapies against skin bacteria and American Tegumentary Leishmaniasis (ATL).</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103970"},"PeriodicalIF":4.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of cell cycle-related gene expression by blue light and its effects on the growth and gametogenesis of Saccharina japonica gametophytes","authors":"Yuanyuan Xu ,&nbsp;Qikun Xing ,&nbsp;Yu Yan ,&nbsp;Fengjuan Zhao ,&nbsp;Haiping Jin ,&nbsp;Yingying Qin ,&nbsp;Xingyu Liao ,&nbsp;Fuli Liu","doi":"10.1016/j.algal.2025.103965","DOIUrl":"10.1016/j.algal.2025.103965","url":null,"abstract":"<div><div>As an economically and ecologically important seaweed inhabiting the subtidal zone, the growth and development of kelp <em>Saccharina japonica</em> are regulated by various environmental factors including blue light. However, studies on the mechanisms underlying the regulation of blue light on kelp gametophyte are still very poor. This study compared the reproductive development rate of <em>S. japonica</em> gametophytes under different blue light exposure durations and found blue light can promote the transition of <em>S. japonica</em> gametophytes from vegetative growth (cell mitosis) to gametogenesis (gamete cell differentiation), with a 12-h blue light exposure being the most efficient. To investigate the role of cell cycle-related genes during gametogenesis of <em>S. japonica</em> gametophytes, Cyclin, CDK, E2F/DP and RBR were first identified in <em>S. japonica</em>. By analyzing cis-acting elements, conserved domains, and phylogenetic relationships of the E2F/DP family, we found that the E2F/DP family had the highest number of light-responsive cis-acting elements and was well conserved in brown algae. RNA-seq data were used to reveal the expression changes of cell cycle-related genes during the gametogenesis. RT-qPCR further corroborated the expression patterns revealed by RNA-seq data, and especially the expression of gene <em>SjRBR</em> fluctuated significantly during the process of blue light-induced gametogenesis. Based on the expression patterns of the aforementioned cell division-related genes at different developmental stages of the gametophyte, we hypothesize that these genes may play a crucial role in regulating the vegetative growth and gametogenesis of the gametophyte. These findings will further enhance our understanding of cell fate determination in multicellular organisms and will also contribute to improving the efficiency of kelp seedling culture techniques based on gametophyte clone.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103965"},"PeriodicalIF":4.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143436959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing astaxanthin production in Schizochytrium sp.: Insights from orthogonal experiments and transcriptome analysis","authors":"Jiaxue Zhao ,&nbsp;Dan Chen ,&nbsp;Chubin Lin ,&nbsp;Xia Wan","doi":"10.1016/j.algal.2025.103966","DOIUrl":"10.1016/j.algal.2025.103966","url":null,"abstract":"<div><div><em>Schizochytrium</em> sp. is a marine microorganism used for the commercial production of docosahexaenoic acid (DHA), with applications in the food and feed industries. In addition to DHA, <em>Schizochytrium</em> sp. has the ability to produce trace amounts of carotenoids, including β-carotene and astaxanthin. This study aims to enhance astaxanthin production in <em>Schizochytrium</em> sp. through optimization of culturing conditions. Contrary to previous reports, supplementation with ethanol or isopropanol did not obviously affect astaxanthin production. Notably, reducing nitrogen levels and replacing glucose with fructose led to a 24-fold increase in astaxanthin production (139.3 μg/g dry cell weight) compared to the lipid fermentation conditions. Transcriptomic analysis indicated that upregulation of genes involved in the mevalonate pathway, including <em>acat</em>, <em>crtE</em>, and <em>crtIBY</em>, alongside the β-carotene hydroxylase gene <em>crtZ</em>, probably contributed to the enhanced astaxanthin production. These findings offer a straightforward and efficient approach for improving astaxanthin production in <em>Schizochytrium</em> sp., thereby enhancing its potential applications in the food and feed industries.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103966"},"PeriodicalIF":4.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143436958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of different processing techniques on microalgal protein extraction","authors":"Catarina Moreira ,&nbsp;Pedro Ferreira-Santos ,&nbsp;Rafaela Nunes ,&nbsp;Bernardo Carvalho ,&nbsp;Hugo Pereira ,&nbsp;José A. Teixeira ,&nbsp;Cristina M.R. Rocha","doi":"10.1016/j.algal.2025.103958","DOIUrl":"10.1016/j.algal.2025.103958","url":null,"abstract":"<div><div>The nutrient-rich composition of microalgae biomass positions it as a highly promising natural food ingredient. This holds the potential to not only enhance the nutritional value of various food products but also simultaneously alter their structural attributes.</div><div>This work investigated the effect of five protein extraction techniques such as freeze-thawing, enzymatic-assisted extraction, high-pressure homogenization, ultrasounds-assisted extraction, and pH adjustment (pH 7, pH 10, and pH 13) in protein yield, and subsequente cell and protein structure of three microalgal suspensions, namely, <em>Chlorella vulgaris</em>, <em>Nannochloropsis oceanica</em>, and <em>Tetraselmis chui</em>.</div><div>In <em>Chlorella vulgaris</em>, freeze- thawing and high-pressure homogenization exhibited a higher effect in terms of protein yield (~26.60 g _protein /100 g _{protein microalgae}). The same occurred for <em>Nannochloropsis oceanica</em> with also ultrasounds-assisted extractions and pH 7 and 10 having a protein yield above 30 %. <em>Tetraselmis chui</em> was similar to <em>Chlorella vulgaris</em> (&gt;20.00 g _protein /100 g _{protein microalgae}) for freeze-thawing, high-pressure homogenization and ultrasound-assisted extraction. Enzymatic-assisted had a the lower protein yield for all the three microalgae (&lt;10.00 g _protein /100 g _{protein microalgae}). The majority of proteins extracted from <em>Chlorella vulgaris</em> samples had molecular weights exceeding 337 kDa, whereas proteins extracted from <em>Nannochloropsis</em> and <em>Tetraselmis</em> had molecular weights ranging from 5 to 50 kDa. α-helices occurred in proteins extracted from <em>Chlorella vulgaris</em> through freeze-thawing and enzymatic-assisted extraction, while <em>Nannochloropsis</em> and <em>Tetraselmis</em> only had β-sheet.</div><div>In conclusion, for optimal protein yield recovery, methodologies such as freeze-thawing and high-pressure homogenization are the most efficient across all studied microalgae. The method selected for extraction had a greater impact on both the protein yield and structure for spray-dried cells.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103958"},"PeriodicalIF":4.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life cycle assessment of protein extraction from microalgae biomass using ohmic heating","authors":"Vitor Sousa ,&nbsp;Roberto Novais ,&nbsp;Teresa M. Mata ,&nbsp;António A. Martins ,&nbsp;Ricardo N. Pereira","doi":"10.1016/j.algal.2025.103962","DOIUrl":"10.1016/j.algal.2025.103962","url":null,"abstract":"<div><div>Life Cycle Assessment (LCA) is an effective tool for assessing the environmental impacts associated with the implementation of innovative processing technologies in biotechnology. This study focuses on the life cycle environmental impacts of thermal protein extraction from microalgae biomass using ohmic heating. <em>Coelastrella</em> was selected as the model organism due to its double cell wall, which presents the worst-case extraction scenario. The environmental impact assessment was conducted using the ReCiPe 2016 method from an equalitarian perspective, encompassing eighteen environmental impact categories. The results demonstrate that applying an electric field of 217 V/cm during protein extraction significantly enhances extraction efficiency while simultaneously promoting a more environmentally sustainable performance. This is achieved through a rapid heating pulse that reaches a maximum temperature of around 100 °C, with a holding time of &lt;1 s. In addition, an alternative energy scenario utilizing entirely renewable energy was evaluated for both the worst and best treatments identified in the LCA study, compared to the reference treatment. The results indicate improvements in at least fifteen of the eighteen indicators assessed under this renewable energy scenario.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103962"},"PeriodicalIF":4.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The growth rate and primary metabolism of Chlamydomonas reinhardtii in batch cultures are affected by acclimation to mixotrophic or autotrophic conditions","authors":"Roman Puzanskiy ,&nbsp;Alexey Shavarda ,&nbsp;Daria Romanyuk ,&nbsp;Maria Shishova","doi":"10.1016/j.algal.2025.103963","DOIUrl":"10.1016/j.algal.2025.103963","url":null,"abstract":"<div><div>The mixotrophic green alga <em>Chlamydomonas reinhardtii</em> is able to grow photoautotrophically as well as uptake acetate as a carbon and energy source. The trophic regime strongly affects the growth rate and metabolism of the algae, what can have long-lasting aftermath even if the conditions have been changed. Mixotrophic pre-cultivation resulted in higher growth rate and physiological activity during the log phase in both mixotrophic and autotrophic cultures of <em>C. reinhardtii</em>. Metabolite profiling showed that acclimation has a pronounced effect on different aspects of primary metabolism, especially during the growth phase. These effects are partly explained by the legacy of previous trophic acclimation. Metabolic effects of acclimation were different in autotrophic and mixotrophic cultures. Common trends of mixotrophic acclimation consisted in higher levels of some carboxylates and amino acids. A pronounced effect of autotrophic acclimation was an increase in sugar accumulation. Also, changing conditions cause a metabolic perturbation expressed in variation of patterns of metabolite content over culture growth. The most stable patterns of dynamics were characteristics of free fatty acids and acylglycerols. Acclimation also affects the functional relationships between metabolite pools, which could be inferred from differences in correlation patterns. Finally, changes in trophic conditions triggered stronger correlations of metabolite content.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103963"},"PeriodicalIF":4.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlamydomonas acidophila for phosphorus recovery from municipal wastewater treatment plants: Effect of light intensity, temperature, different wastewaters and long-term semi-continuous feeding","authors":"Lena S.D. Procopio,&nbsp;Ania Escudero,&nbsp;Colin Hunter","doi":"10.1016/j.algal.2025.103941","DOIUrl":"10.1016/j.algal.2025.103941","url":null,"abstract":"<div><div>Recent years have witnessed a growing scientific and regulatory focus on phosphorus (P) in aquatic environments due to its role in accelerated algae growth, negatively impacting water quality. Agriculture and wastewater treatment plants (WWTPs) stand out as major P sources, prompting tightened discharge standards to meet the European Union Water Framework Directive objectives. Microalgae offer a solution for nutrient recovery, but challenges in mass cultivation and light availability persist. This study explores <em>Chlamydomonas acidophila</em>, an extremophilic microalgae, as a viable option for P recovery from WWTPs by understanding the effect of different light intensities and temperatures, as well as the effect of different wastewater characteristics, on <em>C. acidophila</em> growth and nutrient uptake.</div><div>Batch assays were conducted using growth media, settled wastewater, and final wastewater at various temperatures (10, 16, 20, 25, and 30 °C) and light intensities (15, 40, and 172 μmol photons m⁻² s⁻¹). Furthermore, two 10 L working volume tanks were fed semi-continuously with a hydraulic retention time (HRT) of three days during 200 days at 70 μmol photons m⁻² s⁻¹.</div><div>The results demonstrated that operational conditions of 20 °C and 40 μmol photons m⁻² s⁻¹ were optimal for <em>C. acidophila</em>'s biomass production and nutrient uptake. This characteristic significantly enhances the economic feasibility of <em>C. acidophila</em>-based wastewater treatment systems by allowing operation under lower light conditions. <em>C. acidophila's</em> adaptability to fluctuating nutrient levels, continuous nutrient consumption at low light, and mitigation of inhibitory effects make it a promising candidate for wastewater treatment. In a long-term semi-continuous treatment, removals of 3.6 mg PO₄³⁻ L⁻¹ d⁻¹, 12 mg NH₄⁺ L⁻¹ d⁻¹, and up to 70 mg NO₃⁻ L⁻¹ d⁻¹ were achieved. It can be therefore concluded that <em>Chlamydomonas acidophila</em> has high nutrient assimilation capacity at low light intensities and could be a potential candidate for long-term wastewater treatment processes.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103941"},"PeriodicalIF":4.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-value compounds in three freshwater green microalgae using nitrogen as an abiotic stressor: A study of the antioxidant potential of ethanolic extracts","authors":"Priscila S. Corrêa ,&nbsp;Maria M. Freitas ,&nbsp;Nídia S. Caetano","doi":"10.1016/j.algal.2025.103964","DOIUrl":"10.1016/j.algal.2025.103964","url":null,"abstract":"<div><div>Microalgae are a rich source of several biomolecules, some of which are recognized for their potential antioxidant activity. In this study, <em>Chromochloris zofingiensis</em>, <em>Koliella longiseta</em>, <em>Schizomeris leibleinii</em> were cultivated in five different media, using NaNO₃ or urea as the nitrogen source in different concentrations. BBM_STD (BBM standard), BBM (−) - NaNO₃ concentration reduced tenfold, BBM (X) - absence of nitrogen, BBM (U) - urea replacing NaNO₃ in nitrogen equivalent, and BBM (U-) - urea concentration reduced tenfold. The microalgae extracts were analysed for their production of carotenoids, chlorophylls, lipids, and phenolic compounds. In addition, antioxidant activity was assessed using three different assays (DPPH• RSA, FICA, and TAC). <em>K. longiseta</em> showed highest carotenoids (3.8 mg·g⁻¹) and chlorophyll (10.8 mg·g^{- 1}) content in BBM_STD (BBM standard). Indeed, the lower nitrogen supply (ten times less than BBM_STD) induced lipid accumulation, reaching a maximum of 45.1 % in <em>C. zofingiensis</em> (BBM (−), less NaNO₃). In contrast, urea (BBM (U), BBM using urea as nitrogen source) enhanced the phenolic contents, achieving 33.2 and 30.8 mg GAE·g⁻¹, in <em>K. longiseta</em> and <em>S. leibleinii</em>, respectively. Highest antioxidant potential was found in <em>C. zofigiensis</em> (43.1 % RSA, 21.7 % FICA, and 214.6 mg GAE·g⁻¹ TAC) and <em>S. leibleinii</em> (27.7 % RSA, 28.8 % FICA, and 253.4 mg GAE·g⁻¹ TAC). Overall, phenolic compounds seemed to have major contribution to the antioxidant activity than pigments and lipids.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103964"},"PeriodicalIF":4.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Livestock wastewater as a microalgae growth medium for potential production of biodiesel in arid areas of Mexico","authors":"Carlos Francisco Moreno-Cruz ,&nbsp;Olivia Tzintzun-Camacho ,&nbsp;Marlon C. Gonzalez-Joaquin ,&nbsp;Xiomara E. Aguilar-Martinez ,&nbsp;Daniel González-Mendoza ,&nbsp;Marisela Martinez-Quiroz","doi":"10.1016/j.algal.2025.103957","DOIUrl":"10.1016/j.algal.2025.103957","url":null,"abstract":"<div><div>Microalgae cultivation using livestock wastewater (LW) has the potential to be used to develop efficient wastewater treatment systems and promote biodiesel production. This study analyzed the correlation between the Human Development Index (HDI) and livestock productivity in Mexico, revealing a positive strong relationship (0.8694 and <em>p</em>-value&lt;0.0001) and highlighting the potential of improved waste management to foster societal development. In addition, this study evaluated the use of LW as a culture medium for microalgae, implementing the strategy of sedimentation and dilution with treated wastewater to enhance its physicochemical suitability. The effects on microalgal growth, COD and NO₃⁻ removal efficiency, and lipid yield were evaluated. The maximum biomass production, indicated by cell concentration, was observed in the synthetic culture medium (BBM) (2.5 × 10⁷ cells mL⁻¹). In comparison, the diluted LW (7PS:3 L) medium was demonstrated as an alternative culture medium, achieving a cell concentration of 1.57 × 10⁷ cells mL⁻¹. The results revealed that LW did not significantly inhibit the microalgae's capacity to reduce COD and nitrogen. Particularly, the cultivated microalgae using LW showed a high lipid yield (207 mg_lipid gram_biomass⁻¹), which was significantly higher than BBM 2 and BBM 6 (<em>p</em>-value &lt;0.0001). Furthermore, the use of LW (7PS:3 L) resulted in a favorable lipid profile, characterized by a high content of total unsaturated fatty acids (85 %). Therefore, this work contributes to the development of a livestock wastewater management strategy and its use as an alternative substrate for biodiesel production in arid areas of Mexico, favoring the environmental sustainability in livestock systems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103957"},"PeriodicalIF":4.6,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}