Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Mixotrophic black-box model for predicting Chlorella vulgaris CA1growth rate and nutrient uptake: A systematic approach","authors":"Masoud Tourang,&nbsp;Shulin Chen","doi":"10.1016/j.algal.2025.103972","DOIUrl":"10.1016/j.algal.2025.103972","url":null,"abstract":"<div><div>Using microalgae to uptake the nutrients is a promising approach for polishing anaerobic digestion effluent (<em>ADE)</em>. However, the low growth rates of autotrophic cultures and inhibition caused by high ammonia concentrations in <em>ADE</em> limit the effectiveness of this strategy. One potential solution is the use of mixotrophic species, which are more tolerant to ammonia. <em>Chlorella vulgaris</em> CA1, a mixotrophic microalgae isolated from dairy lagoon wastewater, was selected as a potential candidate. To optimize biomass productivity and associated nutrient removal, a predictive mathematical model was developed. Two-way <em>ANOVA</em> and <em>RSM</em> analysis were used to analyze the effects and interactions of key factors on performance responses. Additionally, an interactive approach was employed to construct a <em>black-box</em> model that describes the inhibitory effects of these factors and their interactions, resulting in an inhibition-type model based on the <em>Andrew</em> equation. The <em>MATLAB Curve Fitting Toolbox</em> was employed to optimize the model parameters and generate the best fit for the experimental data. The results demonstrated that acetate plays a crucial role in mitigating the ammonia toxicity. In the absence of acetate, even very low ammonia concentrations (0.1g L⁻¹) had an inhibitory effect on growth. The optimal initial concentrations of acetate and ammonia were 5.4 and 0.1g L ⁻¹, respectively, while higher concentrations (6.3 g L⁻¹acetate and 2.67 g L⁻¹ ammonia) inhibited growth, consistent with experimental findings (<em>RMSE</em> = 0.045, <em>R</em>^<em>2</em> = 0.96). The study highlights a trade-off between optimizing anaerobic digestion for biogas production and leveraging <em>ADE</em> for microalgal cultivation. Utilizing residual acetate as an organic carbon source under mixotrophic conditions not only enhances biomass productivity but also improves <em>ADE</em> treatment by reducing residual ammonia and acetate levels. Furthermore, the developed model provides a reliable tool for predicting biomass production and nutrient uptake during the initial stages of cultivation, with potential for further refinement in continuous systems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 103972"},"PeriodicalIF":4.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate non-invasive quantification of astaxanthin content using hyperspectral images and machine learning","authors":"Marco L. Calderini ,&nbsp;Salli Pääkkönen ,&nbsp;Aliisa Yli-Tuomola ,&nbsp;Hemanta Timilsina ,&nbsp;Katja Pulkkinen ,&nbsp;Ilkka Pölönen ,&nbsp;Pauliina Salmi","doi":"10.1016/j.algal.2025.103979","DOIUrl":"10.1016/j.algal.2025.103979","url":null,"abstract":"<div><div>Commercial cultivation of <em>Haematococcus pluvialis</em> to produce natural astaxanthin has gained significant traction due to its application in feeds, cosmetics and nutraceuticals. However, monitoring of astaxanthin content in cultures remains challenging and requires invasive, time consuming and expensive approaches. Here, we employed reflectance hyperspectral imaging (HSI) of <em>H. pluvialis</em> suspensions within the visible spectrum, combined with a 1-dimensional convolutional neural network (CNN) to predict the astaxanthin content (μg mg⁻¹) quantified by high-performance liquid chromatography (HPLC). This approach had low average prediction error (5.9 %) for samples cultured under the same conditions and was only unreliable at very low astaxanthin contents (&lt;0.6 μg mg⁻¹). In addition, our CNN model outperformed single or dual wavelength linear regression models even when the spectral data was obtained with a spectrophotometer coupled with an integrating sphere. Different cultivation conditions to the ones used for the training of the CNN model affected the reflectance spectra of <em>H. pluvialis</em> cultures, leading to higher prediction errors (32.9 %). Transfer learning, a partial re-training of the model, fine-tunned the CNN to the variability observed in the new samples leading to a lower average prediction error (8.2 %). Overall, this study proposes the use of HSI in combination with a CNN for precise non-invasive quantification of astaxanthin in cell suspensions. Optimal performance is achieved when the training dataset accurately reflects the inherent variability of the target samples to be quantified.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"87 ","pages":"Article 103979"},"PeriodicalIF":4.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143529392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-step sustainable production of activated carbon from microalgal biomass: An approach to waste recovery from urban wastewater","authors":"Renata Estevam ,&nbsp;Ricardo Franci Gonçalves ,&nbsp;Daniel Fernandes Cipriano ,&nbsp;Janaína Simões Lima ,&nbsp;Miguel Ângelo Schettino Junior","doi":"10.1016/j.algal.2025.103977","DOIUrl":"10.1016/j.algal.2025.103977","url":null,"abstract":"<div><div>The growing need for sustainable waste management and the increasing demand for eco-friendly materials have driven the exploration of microalgae as a renewable resource for activated carbon production. Microalgae offer advantages such as rapid growth, high photosynthetic efficiency, and the ability to utilize wastewater for cultivation, making them an appealing alternative to traditional biomass sources. Activated carbon was produced from microalgal biomass grown in high-rate aerobic ponds fed with the effluent from an up-flow anaerobic sludge blanket reactor treating urban wastewater. The microalgal biomass was subjected to different harvesting (physical, flocculation, and coagulation) and pretreatment (thermal and thermochemical) methods to investigate the influence of these factors on the physicochemical properties of activated carbons. Fourier-transform infrared spectroscopy showed that biomass pretreatment influenced the composition of activated carbons. Pretreatment reduced yield by up to 5 % and increased the thermostability of activated carbons, indicating a reduction in the presence of less recalcitrant compounds. The combined use of Tanfloc® for biomass harvesting and thermochemical pretreatment resulted in a high specific surface area, as observed for ACT-pH 3 (415 m²/g) and ACT-pH 8 (382 m²/g). This study provides recommendations for optimizing the production of activated carbon from microalgal biomass grown on urban wastewater, offering a route to waste recycling and promising prospects for the sustainable development of activated carbon materials.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"87 ","pages":"Article 103977"},"PeriodicalIF":4.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPTLC hyphenated with planar effect-directed assays (EDA) for the rapid screening and evaluation of macro algae fermentation with respect to their antioxidant and anti-inflammatory activity","authors":"Snezana Agatonovic-Kustrin ,&nbsp;Sheryn Wong ,&nbsp;Anton V. Dolzhenko ,&nbsp;Vladimir Gegechkori ,&nbsp;David W. Morton","doi":"10.1016/j.algal.2025.103978","DOIUrl":"10.1016/j.algal.2025.103978","url":null,"abstract":"<div><div>Marine algae or seaweed are among the ocean's most valuable sources of bioactive compounds. They grow abundantly in harsh marine environments of high salinity, high oxygen concentrations and extreme sunlight. The lack of oxidative damage in their structural components indicates the presence of highly effective antioxidants and anti-inflammatory compounds.</div><div>This study examines the effect of microbial fermentation on the phytochemical composition and bioactivity in different solvent extracts from marine algae via HPTLC microchemical derivatization and HPTLC bioautography. Microchemical derivatization with anisaldehyde/sulfuric acid was used to assess the extract complexity, DPPH free radical assay to estimate and detect antioxidant activity, and enzymatic bioassay to detect and estimate anti-inflammatory activity via COX-1 enzyme inhibition. The red alga sample was selected for further characterisation due to higher amounts of bioactive compounds.</div><div>The potency of the extract with the highest COX-1 inhibition was evaluated, with the high-maximal inhibitory concentrations (IC₅₀) determined by an HPTLC-based experimental procedure developed in our lab. The IC₅₀ values for COX-1 enzyme inhibition from both fermented and nonfermented extracts were significantly lower compared to the IC₅₀ for salicylic acid as a reference standard. Six chromatographic zones exhibited bioactivity: 1,3,4, 6–8 zones with antioxidant activity and 4, 6–8 zones with anti-inflammatory activity. Compounds from bioactive zones were isolated using preparative TLC. They were further characterised using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, and liquid chromatography-mass spectrometry (LC-MS).</div><div>The main constituents identified as responsible for antioxidant activity were polypeptides (zone 1), hydrolysed lignin (zone 3), carotenoids (zone 4), macrocyclic 4-pyrones (zone 6), fatty acids (zones 6 and 7) and their diglycerides (zone 8).</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"87 ","pages":"Article 103978"},"PeriodicalIF":4.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A special lycopene ε-cyclase from Chromochloris zofingiensis ATCC 30412 with multiple catalytic functions","authors":"Xing Meng ,&nbsp;Xi Li ,&nbsp;Ruijuan Ma ,&nbsp;Wenjin Lin ,&nbsp;Yiping Zou ,&nbsp;Baobei Wang ,&nbsp;Shih-Hsin Ho ,&nbsp;Jianfeng Chen ,&nbsp;Youping Xie","doi":"10.1016/j.algal.2025.103974","DOIUrl":"10.1016/j.algal.2025.103974","url":null,"abstract":"<div><div>The cyclization of lycopene catalyzed by lycopene cyclases (LCYs) represents a pivotal step in forming α-branch or β-branch carotenoids. However, the catalytic functions of LCYs are species-specific. In this study, the LCYs of <em>Chromochloris zofingiensis</em> ATCC 30412, including lycopene ε-cyclase (CzLCYE) and lycopene β-cyclase (CzLCYB), were characterized by sequence alignment, subcellular localization, and function identification. The sequence alignment revealed the presence of several conserved motifs in CzLCYE and CzLCYB, exhibiting high similarity to the LCYs of other organisms. Subcellular localization experiments illustrated the chloroplast localization of these two enzymes. Functional complementation assays in <em>Escherichia coli</em> demonstrated that CzLCYB exhibited merely β-cyclase activity. Interestingly, CzLCYE displayed strong ε-monocyclase activity, moderate β-monocyclase activity, and weak β, β-bicyclase activity, which are rarely found in other organisms. Further, the structures of CzLCYE complexes were built to identify the essential amino acid residues within the substrate-binding pocket. Site-directed mutagenesis experiments revealed the significant roles of TYR140, PHE321, and THR337 in maintaining CzLCYE activity as well as LEU236, PHE245, PHE405, and LEU488 in β-ring formation. However, the mutation of THR205 to ALA enhanced β, β-bicyclase activity. These findings reveal the special catalytic functions of CzLCYE, contributing to a deeper understanding of carotenoid biosynthesis in <em>C. zofingiensis</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"87 ","pages":"Article 103974"},"PeriodicalIF":4.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of oligosaccharides from Enteromorpha clathrata on tolerance of wheat (Triticum aestivum L.) seedings to saline-alkali stress","authors":"Yinglu Wu ,&nbsp;Chunying Du ,&nbsp;Rui Xiao ,&nbsp;Bo Zhu ,&nbsp;Xiuchao Song ,&nbsp;Xinyu Wang ,&nbsp;Yimin Qin ,&nbsp;Peng Wang","doi":"10.1016/j.algal.2025.103975","DOIUrl":"10.1016/j.algal.2025.103975","url":null,"abstract":"<div><div>Polysaccharides from algae have received considerable attention owing to their ability to enhance crop tolerance to abiotic stress effectively. In this study, four oligosaccharides (EC1-EC4) of different molecular weights (11.66, 3.55, 2.21, and 1.61 kDa), respectively) enzymatically cleaved from <em>Enteromorpha clathrata</em> were applied to wheat seedlings (<em>Triticum aestivum</em>) under saline-alkali stress. Their effects on enhancing plant tolerance to saline-alkali stress were investigated at 24 h and 120 h and compared in terms of alleviating oxidative stress, osmotic stress, ion toxicity, and stimulation of abscisic acid homeostasis. The application of EC3 (2.21 kDa) for 120 h reduced the malondialdehyde content by 11.0 % in leaves and 27.2 % in roots compared with those in the negative control (Na) group. The EC4 (1.61 kDa) group showed reductions of 15.4 % and 23.6 %, respectively. Treatment with EC3 increased the peroxidase enzyme activity enhancement of roots to 1051.6 U·gFw⁻¹·min⁻¹ compared to 887.7 gFw⁻¹·min⁻¹ in the Na group. Its application was the most effective in enhancing proline accumulation and synthesis. Several aquaporins were further up-regulated to better defend against osmotic stress, following EC2 treatment (3.55 kDa) treatment. In addition, the expression of several ionotropic proteins was significantly enhanced in cells treated with EC3 maintain ion homeostasis and reduce ion toxicity. Furthermore, EC3 stimulated abscisic acid (ABA) synthesis and ABA-dependent signaling to enhance saline-alkali resistance in wheat seedlings. In conclusion, the oligosaccharide with 2.21 kDa from <em>E. clathrata</em> represents an effective way to improve the resistance of wheat seedlings to saline-alkali stress.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 103975"},"PeriodicalIF":4.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical, radical–scavenging, and anti-proliferative analyses of enzyme-assisted extracted polysaccharides unravel the potential of tropical green seaweeds","authors":"Babita Choudhary,&nbsp;Jaykumar Patel,&nbsp;Avinash Mishra","doi":"10.1016/j.algal.2025.103973","DOIUrl":"10.1016/j.algal.2025.103973","url":null,"abstract":"<div><div>This study focused on enzyme-assisted extraction of polysaccharides from four selected tropical green seaweeds (<em>Caulerpa scalpelliformis, Ulva fasciata, Ulva lactuca,</em> and <em>Acrosiphonia orientali</em>s). The polysaccharides were physicochemically characterized by using advanced analytical tools and also evaluated for their scavenging and anti-proliferative activities. Enzyme-assisted extraction yielded a higher polysaccharide content compared with traditional techniques. The extracted crude and purified polysaccharides were rich in sugars, including galactose and mannose. Functional group analysis based on Fourier-transform infrared spectroscopy confirmed the presence of characteristic polysaccharide groups, such as hydroxyl, carboxyl, and sulfate. The extracted polysaccharides were amorphous in nature with a fibrous and porous morphology, showing uneven aggregates. Different biological tests, including the ferric reducing antioxidant power and phosphomolybdate assays, revealed significant antioxidant activity. Furthermore, the anti-proliferative studies performed on two cancer cell lines (Huh-7 and HeLa) showed different degrees of inhibition, with certain purified fractions showing higher efficacy. Overall, the results indicate that these extracted polysaccharides have potential applications in nutraceutical, pharmaceutical, and therapeutic products, particularly as antioxidants and anti-cancer agents.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"87 ","pages":"Article 103973"},"PeriodicalIF":4.6,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simple growth model of Saccharina latissima cultivated in Scandinavia: Theoretical and practical implications","authors":"Niels Holst ,&nbsp;Teis Boderskov ,&nbsp;Annette Bruhn","doi":"10.1016/j.algal.2025.103971","DOIUrl":"10.1016/j.algal.2025.103971","url":null,"abstract":"<div><div>Our purpose was to construct the simplest possible, biologically-based model that could simulate the biomass growth from autumn to summer of <em>Saccharina latissima</em> cultivated on lines in Scandinavia. We developed the model based on a well-established framework for modelling terrestrial crops, using data from an experiment that we ran for one growing season with <em>S. latissima</em> cultivated on lines in Limfjorden, Denmark. The model was subsequently compared to two independent data sets obtained from previously published studies carried out in Denmark and Norway. Surprisingly, sea temperature was not needed as a driving variable to obtain a model as accurate as earlier models; global irradiation was the only environmental variable necessary to drive the model. Model outputs compared favourably to the independent Norwegian data set, less so to the independent Danish data set. In general, the predicted time trends of <em>S. latissima</em> biomass mimicked both the pattern and the magnitude of the observed biomass. Due to a lack of data, the model did not include the effects of sub-optimal levels of nutrients or salinity; however, thanks to the model's simplicity (it can be formulated in just one line) and its reliance upon basic biological principles, we expect that it can easily be re-calibrated to simulate growth also under sub-optimal conditions. Its plain formulation provides a readily available prediction of the biomass production to be expected from <em>S. latissima</em> cultivation. Moreover, the simplicity of the model makes it straightforward to incorporate as a component in more complex models of marine cropping systems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"87 ","pages":"Article 103971"},"PeriodicalIF":4.6,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-flocculated Picochlorum maculatum MACC3 as a functional feed for improved growth and health in guppies (Poecilia reticulata)","authors":"Kannukkarathi Thasreefa,&nbsp;Jayesh Puthumana,&nbsp;I.S. Bright Singh,&nbsp;Joseph Valsamma","doi":"10.1016/j.algal.2025.103968","DOIUrl":"10.1016/j.algal.2025.103968","url":null,"abstract":"<div><div>Microalgae offer a sustainable alternative to traditional aquaculture feeds, promoting enhanced growth performance and disease resistance. This study evaluated the effects of dietary supplementation with chitosan-flocculated biomass of the marine picoalga <em>Picochlorum maculatum</em> MACC3 on growth, digestive enzyme activity, immunity, ornamentation, and gut microbiome composition in juvenile Silverado guppies (<em>Poecilia reticulata</em>). Two groups were studied for 120 days: a control group fed freeze-dried bloodworm meal and a treatment group fed the same diet supplemented with 10 % chitosan-flocculated, freeze-dried <em>P. maculatum</em> MACC3. The supplemented group showed significant (<em>p &lt;</em> 0.05) improvements in growth, survival, and digestive enzyme activities (proteases, amylases, and lipases), with a non-significant (<em>p</em> &gt; 0.05) increase in alkaline phosphatase activity. Upon challenge with <em>Aeromonas salmonicida</em>, the treatment group exhibited a 46.67 % higher survival rate (<em>p &lt;</em> 0.05) than the control group. Immunological markers, including total protein, immunoglobulin, alkaline phosphatase, protease, and bactericidal activities, were significantly (<em>p &lt;</em> 0.05) enhanced in algal biomass-fed fish. Skin pigmentation and ornamentation were also significantly (<em>p &lt;</em> 0.05) enhanced, with increased carotenoid, pteridine, and melanin levels and a 10.23-fold higher expression of the pigment pattern-related <em>csf1ra</em> gene. Colordistance image analysis confirmed two distinct clusters, showing clear pigmentation differences between the experimental groups. Gut microbiome analysis revealed a significantly (<em>p &lt;</em> 0.05) higher abundance of probiotic genera (<em>Weissella</em>, <em>Lactobacillus</em>, and <em>Streptococcus</em>) in the treatment group, while the control group was dominated by pathogenic genera, with PICRUSt predictions indicating more disease-related pathways. The treatment group also had a lower Bacteroidetes to Firmicutes ratio than the control, which was correlated with the observed growth benefits. In conclusion, <em>P. maculatum</em> MACC3 biomass is an effective functional feed for ornamental fish, enhancing growth, digestion, immunity, and pigmentation through gut microbiome modulation. The findings underscore the potential of <em>P. maculatum</em> MACC3 biomass as a sustainable feed ingredient in aquaculture.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103968"},"PeriodicalIF":4.6,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iodine reduction and retention of nutrients and flavour-active compounds upon warm seawater treatment of the kelps Alaria esculenta and Saccharina latissima","authors":"Pierrick Stévant ,&nbsp;Cecilie Bay Wirenfeldt ,&nbsp;Janne Stangeland ,&nbsp;David Cohen ,&nbsp;Jens J. Sloth ,&nbsp;Susan Løvstad Holdt ,&nbsp;Arne Duinker","doi":"10.1016/j.algal.2025.103969","DOIUrl":"10.1016/j.algal.2025.103969","url":null,"abstract":"<div><div>Commercially cultivated kelp (seaweed) species represent a potential dietary source of iodine but may also put consumers at risk of excessive intakes upon frequent consumption. This study investigated warm seawater (W-SW) treatment as a simple method for reducing the iodine content of <em>Alaria esculenta</em> and <em>Saccharina latissima</em>. Iodine concentrations decreased in both kelps upon W-SW exposure at 45 °C, i.e. by 38 % and 78 %, respectively, in <em>A. esculenta</em> and <em>S. latissima</em> after 1 min and 51 % and 88 % after 2 min. Longer treatments resulted in further decrease in <em>A. esculenta</em> whereas only marginal further reduction in iodine concentrations were achieved in <em>S. latissima</em>. No reduction in iodine concentration was measured in <em>S. latissima</em> following treatment at 35 °C (not tested for <em>A. esculenta</em>). W-SW treatment at 45 °C induced loss of biomass in both kelps although the total retention was notably higher in <em>A. esculenta</em> compared to <em>S. latissima</em>. Among the analysed macronutrients, potassium and mannitol were associated with the lowest retentions. Losses of micronutrients (incl. vitamin B1 and B9) and trace elements were also measured in both kelps. The retention of free glutamate was high in both species suggesting that W-SW exposure does not negatively affect the umami potential of the final ingredients for food. Based on portions contributing 600 μg iodine, W-SW-treated <em>A. esculenta</em> was more nutritious than a comparable ingredient from <em>S. latissima</em>, with nutritionally relevant contribution (&gt; 15 % of dietary reference intake (DRI)) per portion (4.6 g dry <em>A. esculenta</em> vs. 1.2 g <em>S. latissima</em>) of sodium, and notable contributions (&gt; 5 % of DRI) of other minerals (calcium, magnesium and potassium) and vitamin B9. Short W-SW treatment is a simple approach that could be implemented in commercial kelp production and contribute to reducing the risk of excessive dietary iodine exposure from seaweed consumption.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103969"},"PeriodicalIF":4.6,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}