Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Potentially commercialisable alga, Coelastrella sp. SDEC-28, for stable growth and multiple product applications in pilot-scale seawater-wastewater cultivation","authors":"Zhen Xie ,&nbsp;Huiying Chen ,&nbsp;Meng Ma ,&nbsp;Mingyan Liu ,&nbsp;Haiyan Pei","doi":"10.1016/j.algal.2025.104057","DOIUrl":"10.1016/j.algal.2025.104057","url":null,"abstract":"<div><div>The use of seawater for low-cost cultivation of freshwater microalgae faces limitations because hyperosmotic environments restrict microalgal growth and reduce metabolite accumulation. This study applied monosodium glutamate wastewater to seawater cultivation (SMGW) for the high-value microalgal strain <em>Coelastrella</em> sp. SDEC-28. With the assistance of aeration, biomass and lipids productivity increased twofold, reaching values of 188.95 and 63.24 mg/L/d in SMGW. The special structure of <em>Coelastrella</em> contributed to the stability of its intracellular environment. Thus, three metabolites—carbohydrates, lipids, and proteins—remained stable in the seawater medium at 28.31%, 36.59%, and 30.52%, respectively. The fatty acids and amino acids profiles highlighted potential for producing biodiesel and health supplements. Finally, the establishment of the first 1000-L microalgal cultivation system for SDEC-28 demonstrated the feasibility of large-scale commercial production. This study highlights SMGW with aeration as a cost-effective strategy for boosting SDEC-28's biomass and lipids yields while stabilizing metabolites and enabling scalable production through a 1000-L cultivation system.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104057"},"PeriodicalIF":4.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Algae-based corrosion inhibitors as innovative approaches to corrosion prevention: A review","authors":"Homa Kahkesh ,&nbsp;Mahdi Yeganeh ,&nbsp;Zahra Shahryari","doi":"10.1016/j.algal.2025.104071","DOIUrl":"10.1016/j.algal.2025.104071","url":null,"abstract":"<div><div>Corrosion poses a significant threat to industries, lives, and property, making its management essential from technical, financial, environmental, and aesthetic perspectives. Corrosion inhibitors, particularly synthetic chemicals, are widely used but often toxic and costly. This has led to increased interest in eco-friendly alternatives, especially green corrosion inhibitors derived from algae extracts. Algae are abundant sources of biodegradable and non-toxic biomaterials, which have shown promise as effective corrosion inhibitors due to their unique chemical properties. Despite extensive research on green-based compounds in recent years, there is a lack of comprehensive reviews focusing specifically on algal extracts as corrosion inhibitors for metals. Existing reviews predominantly focus on terrestrial plant-based inhibitors or other bio-sourced materials, with only limited studies addressing algae-derived inhibitors. These studies are typically restricted to specific algae species or narrow experimental conditions, lacking comprehensive synthesis. This review uniquely bridges this research gap by providing a detailed analysis of both microalgae (e.g., <em>Chlorella</em>, <em>Spirulina, etc</em>) and macroalgae (e.g., <em>Sargassum, etc</em>) as sustainable corrosion inhibitors for ferrous alloys across various corrosive environments, including acidic and saline media. It systematically explores their mechanisms of action, advantages, limitations, and synergistic potential with other corrosion inhibitors. Furthermore, the present paper incorporates a comparative analysis of algae-derived inhibitors with traditional ones from various research studies, highlighting their distinct inhibition efficiency, biochemical properties, sustainability, and versatility. By addressing challenges such as compositional variability, stability, and scalability, this paper underscores the transformative role of algae-based inhibitors and encourages innovation in corrosion prevention strategies. The integration of recent findings consolidates evidence for their application in diverse corrosive media, positioning algae-derived inhibitors as a promising solution for sustainable industrial use.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104071"},"PeriodicalIF":4.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remarkable metabolic reconfiguration due to three classical nitrogen sources in Gracilariopsis lemaneiformis (Rhodophyta)","authors":"Wenkang Fan ,&nbsp;Chunyou Yang ,&nbsp;Dingzhi Song ,&nbsp;Peilin Ye ,&nbsp;Honghao Liang ,&nbsp;Chunping Huang ,&nbsp;Muhamad Syaifudin ,&nbsp;Chenhui Zhong ,&nbsp;Tangcheng Li ,&nbsp;Hong Du","doi":"10.1016/j.algal.2025.104069","DOIUrl":"10.1016/j.algal.2025.104069","url":null,"abstract":"<div><div>The macroalga <em>Gracilariopsis lemaneiformis</em> undergoes dynamic nitrogen (N) fluctuations during cultivation, driven by climate change and eutrophication. However, our understanding of how <em>G. lemaneiformis</em> responds to different N environments remains limited. This study investigates the physiological and molecular metabolic responses of <em>G. lemaneiformis</em> to various N chemical forms (ammonium, nitrate, and urea). Our results indicate that <em>G. lemaneiformis</em> preferentially utilizes ammonium, consuming it more rapidly than nitrate and urea. Furthermore, the transcript profiles of N transporters exhibited distinct regulatory patterns between inorganic N and urea. Metabolic flux analysis revealed significant changes under different conditions of N. Notably, ammonium treatment upregulated the expression of photosynthesis-related proteins in PS II and led to an accumulation of amino acids with higher N content, such as arginine and citrulline. In contrast, nitrate treatment downregulated carbon (C) fixation while promoting the accumulation of amino acids with higher C/N ratios, particularly leucine and valine. Urea treatment resulted in elevated levels of polyamines and aromatic amino acids, which displayed the highest C/N ratios. As a result, the overall C/N ratio in <em>G. lemaneiformis</em> corresponded with that of the dominant amino acids across the three N treatments. Our findings revealed that ammonium, nitrate, and urea differentially affect C and N allocation in <em>G. lemaneiformis</em>. Furthermore, strategic use of specific forms of N can significantly enhance the production of industrially valuable metabolites, highlighting the potential of this macroalga as a sustainable resource for aquaculture and nutraceutical applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104069"},"PeriodicalIF":4.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an estimation tool for monitoring growth and starch storage in microalgae cultures based on gas balance analysis","authors":"Fernando Robert Ferrel Ballestas ,&nbsp;Hélène Marec ,&nbsp;Jack Legrand ,&nbsp;Mariana Titica ,&nbsp;Guillaume Cogne","doi":"10.1016/j.algal.2025.104066","DOIUrl":"10.1016/j.algal.2025.104066","url":null,"abstract":"<div><div>Extensive research has been conducted on using microalgae biomass as a feedstock for biofuel production. Despite this, effective and accessible monitoring tools are still needed to track key energetic molecules, such as carbohydrates and lipids, to enhance and optimize the production of enriched biomass. This study presents the development and assessment of a three-compartment stoichiometric model designed as a monitoring tool for on-line estimation of growth kinetics and carbon reserve accumulation, primarily in the form of starch and, at later stages, triglycerides (TAGs). The proposed method leverages the indirect measurement of the net oxygen production rate and stoichiometric relationships governing the metabolic transitions within the bioprocess. It considers three key biomass components – catalytic biomass/cell structural components, starch, and TAGs – which accumulate sequentially over time during batch cultures under nitrogen-limiting conditions. The metabolic shift during nitrogen deprivation was modeled using well-defined kinetic terms. Model parameters were identified from two progressively nitrogen-starved batch cultures of <em>Chlamydomonas reinhardtii</em> and evaluated on an independent batch culture. While the model successfully captures key trends in nitrogen uptake, biomass formation, and carbon reserve accumulation (primarily as starch), its applicability to larger-scale systems with more complex environmental conditions remains to be further investigated. Nonetheless, this approach provides a promising foundation for real-time monitoring and bioprocess control strategies in microalgal cultivation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104066"},"PeriodicalIF":4.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a co-culture system of Bacillus aryabhattai and Desmodesmus sp. and its performance in treating simulated wastewater with high nitrogen and phosphorus","authors":"Jianmei Dong ,&nbsp;Shuting Peng ,&nbsp;Xiaoyun Zhang ,&nbsp;Zijian Xie ,&nbsp;Ruirui Meng ,&nbsp;Yuqin Xi ,&nbsp;Weibao Kong","doi":"10.1016/j.algal.2025.104070","DOIUrl":"10.1016/j.algal.2025.104070","url":null,"abstract":"<div><div>The algae-bacteria co-culture system has emerged as a promising approach for biological wastewater treatment. In this symbiotic system, microalgae supply oxygen through photosynthesis, while bacteria reciprocate by providing inorganic and organic nutrients via metabolic processes, enabling synergistic pollutant removal. However, existing studies remain limited in scope, primarily focusing on specific microbial species. In this study, a novel algae-bacteria co-culture system was established using tolerant and dominant algae and bacteria species isolated from a wastewater treatment plant. The growth characteristics and pollutant removal performance of this system were evaluated in simulated wastewater with high nitrogen and phosphorus levels. The results indicated that <em>Bacillus aryabhattai</em>-<em>Desmodesmus</em> sp. (<em>Ba</em>-<em>Ds</em>) co-culture system achieved optimal removal efficiencies for NH₄⁺-N, total phosphorus (TP), and chemical oxygen demand (COD) under conditions of an algae-bacteria inoculate ratio of 5:1, N/P ratio of 6, and pH of 7. The removal rates reached 30.60 %, 49.30 %, and 93.73 %, respectively. These findings highlight the system's enhanced pollutant removal capacity and demonstrate scalable potential for industrial wastewater treatment applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104070"},"PeriodicalIF":4.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From seaweeds to skincare: Unveiling the dermatological potential of Bifurcata bifurcata","authors":"Inês Luz ,&nbsp;Celso Alves ,&nbsp;Joana Silva ,&nbsp;Alice Martins ,&nbsp;Candela Alvarez ,&nbsp;Patrícia Susano ,&nbsp;Susete Pintéus ,&nbsp;Helena Gaspar ,&nbsp;Rui Pedrosa","doi":"10.1016/j.algal.2025.104068","DOIUrl":"10.1016/j.algal.2025.104068","url":null,"abstract":"<div><div>Society has become more aware of the significance of a healthy skin, resulting in a growing need for natural and innovative skin care products. Seaweeds are known to be a rich source of bioactive compounds with a wide range of biotechnological applications. Therefore, the aim of this study was to evaluate the dermatological potential of six fractions (F1-F6) obtained from the brown seaweed <em>Bifurcaria bifurcata</em>. The antioxidant, anti-enzymatic, cytotoxic, photoprotective, wound healing, and antimicrobial properties were studied. The ethyl acetate fraction (F4) displayed the best ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (EC₅₀: 35.7 μg/mL), also demonstrating the highest anti-enzymatic activity, inhibiting collagenase and tyrosinase activities with an IC₅₀ value of 33.6 μg/mL and 50.1 μg/mL, respectively. The hydrophilic fraction (F5) exhibited the highest photoprotective capacity, reducing the reactive oxygen species (ROS) production by 30.9 % on HaCaT cells exposed to UVA/B radiation compared with the vehicle. By contrast, the solid water-insoluble fraction (F2) promoted the highest wound healing area (47.3 %). Finally, the cyclohexane fraction (F3) showed the highest antimicrobial activity, inhibiting <em>Staphylococcus epidermidis</em> (48.5 % at 200 μg/mL) and <em>Cutibacterium acnes</em> (IC₅₀: 59.0 μg/mL) growth. Based on these results, <em>B. bifurcata</em> reveals to be a promising source of bioactive ingredients for the development of innovative multitarget dermatological products.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104068"},"PeriodicalIF":4.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the efficacy and safety of omega-3 fatty acid nutritional supplements from Schizochytrium sp. in dog food","authors":"Zi-Xu Zhang ,&nbsp;Yan-Cheng Lin ,&nbsp;Min Lian ,&nbsp;Ying-Feng Li ,&nbsp;Jin-Fa Chen ,&nbsp;Xue-Lian Ma ,&nbsp;Dong-Sheng Guo ,&nbsp;Guang Yang ,&nbsp;Xiao-Man Sun","doi":"10.1016/j.algal.2025.104072","DOIUrl":"10.1016/j.algal.2025.104072","url":null,"abstract":"<div><div>The rising demand for omega-3 polyunsaturated fatty acids (n-3 PUFAs) and concerns over fish oil sustainability have driven the search for alternatives. This study evaluated the efficacy and safety of algal powder and algal oil from <em>Schizochytrium</em> sp. as n-3 PUFAs supplements in dogs, compared to fish oil. Twenty-four Beagle dogs were fed diets supplemented with 1 % fish oil, 1 % algal powder, 1 % algal oil, or no n-3 PUFAs for 28 days. Results showed that algal-derived supplements significantly increased serum DHA levels (up to 0.265 ± 0.05 g/l), improved coat quality, and enhanced antioxidant capacity. Notably, algal powder exhibited the strongest lipid-lowering effects, reducing total cholesterol to 69.4 % of control levels, despite its lower EPA content. Safety function markers (alanine transferase, alkaline phosphatase, and acid phosphatase) remained stable across all groups, confirming safety. These findings highlight algal powder and algal oil as effective, safe, and sustainable n-3 PUFA sources, offering a viable alternative to fish oil for canine nutrition.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104072"},"PeriodicalIF":4.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and molecular insights into color variation and light intensity adaptation in Eucheuma perplexum with applications for selective cultivation","authors":"Ji-Long Liao ,&nbsp;Zih-Ning Ding ,&nbsp;Ya-Han Hsu ,&nbsp;Jui-Sheng Chang","doi":"10.1016/j.algal.2025.104067","DOIUrl":"10.1016/j.algal.2025.104067","url":null,"abstract":"<div><div>Red algae exhibit remarkable color diversity. However, the physiological and molecular mechanisms driving these color variations remain incompletely elucidated. This study elucidates the physiological and molecular mechanisms underlying color variation in <em>Eucheuma perplexum</em>, with a focus on the red and green morphotypes observed during cultivation. Our research revealed significant differences in pigment content, growth rate, and carrageenan yield between the two morphotypes. Notably, the green morphotype exhibited lower phycoerythrin (PE) content compared to the red morphotype, directly contributing to the observed color differences. RNA-seq analysis revealed significant differential expression of genes, particularly those involved in photosynthesis. Light intensity experiments demonstrated that increased illumination induced phycobiliprotein restructuring, characterized by a pronounced reduction in PE content and significant upregulation of photosynthesis-related genes. These findings indicate that the color variation in <em>E. perplexum</em> is closely linked to adaptation to light intensity, with the green morphotype employing distinct strategies to manage light-induced stress, including increased phenolic compound production and reorganization of photosynthetic pigments. This study provides novel molecular insights into the mechanisms driving color variation in red algae and underscores the potential for selectively cultivating specific morphotypes to optimize production for targeted applications, such as enhancing carrageenan yield or tailoring pigment composition.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104067"},"PeriodicalIF":4.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential responses of Microcystis aeruginosa to single and repeated exposure to Myriophyllum spicatum extracts","authors":"Qing Cao,&nbsp;Bensheng You,&nbsp;Haibo Xu,&nbsp;Weijing Liu,&nbsp;Shuzhan Ma","doi":"10.1016/j.algal.2025.104060","DOIUrl":"10.1016/j.algal.2025.104060","url":null,"abstract":"<div><div>Allelochemicals have been proven to be effective in inhibiting microalgae. However, there is limited knowledge regarding their effect on cyanobacteria in the case of repeated exposure, which is more environmentally relevant. In the present study, the effects of multiple low-dosage additions and single high-dosage addition of <em>Myriophyllum spicatum</em> extracts toward <em>Microcystis aeruginosa</em> were compared based on cell density, photosynthetic response, antioxidative response, and microcystins (MCs) production. Although the cumulative exposure content of <em>M. spicatum</em> extracts was the same (5 g/L), our results showed that algal growth was promoted in repeated low-dosage exposure, whereas single high-dosage exposure inhibited cell growth and decreased photosynthetic activity with the highest inhibition rate of cell density reaching 58.2 %. Oxidative stress occurred in all treatments exposed to <em>M. spicatum</em> extracts, as indicated by the increased catalase (CAT) activity and superoxide Dismutase (SOD) activity. It is noteworthy that oxidative damage to the cell membrane only occurred in single high-dosage exposure as indicated by the malondialdehyde (MDA) contents, which were 1.9, 2.1, 2.8, and 2.7 times higher than that of the control group from day 2 to day 5, respectively. Moreover, repeated low-dosage exposure could promote MC synthesis as indicated by the up-regulated <em>mcyD</em> gene abundance. Cell lysis may be responsible for the increased MCs release in single high-dosage exposure treatment since active transport of MCs was suppressed as indicated by <em>mcyH</em> gene abundance. This study revealed differential responses of <em>M. aeruginosa</em> to single and repeated exposure to allelochemicals, shedding light on the allopathy control of cyanobacteria.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104060"},"PeriodicalIF":4.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprospecting microalgal strains cultivated using bicarbonate under a pH-regulated regime for enhanced carbon capture and utilization","authors":"Mary Joy D. Latagan ,&nbsp;Dillirani Nagarajan ,&nbsp;Jih-Heng Chen ,&nbsp;Wei-Ming Huang ,&nbsp;Chun-Yen Chen ,&nbsp;Melvin R. Pacquiao ,&nbsp;Mark Daniel G. de Luna ,&nbsp;Analiza P. Rollon ,&nbsp;Jo-Shu Chang","doi":"10.1016/j.algal.2025.104064","DOIUrl":"10.1016/j.algal.2025.104064","url":null,"abstract":"<div><div>Microalgae offers significant potential as a sustainable resource for biofuel production. However, an inefficient supply of gaseous CO₂ can lead to carbon limitation due to poor mass transfer efficiency, which poses a significant challenge in large-scale microalgal cultures. This study investigates pH-stat bicarbonate-based cultivation of various microalgal strains aimed at carbohydrate and lipid production. Four green algal strains were assessed for their capability to utilize bicarbonate ions as their inorganic carbon source for photosynthesis. Among the strains, <em>Chlamydomonas</em> sp. JSC4 attained the highest biomass concentration (1.05 ± 0.05 g/L), biomass productivity (242 mg/L/day), lipid content (42.7 ± 0.85 %), and carbohydrate content (39.16 ± 0.09 %) under conditions of 50 mM KHCO₃, pH 8, and 7 days of cultivation. The biomass productivity attained in this study is higher than those in the reported literature. The predicted biodiesel quality for all strains met the ASTM <span><span>D6751</span><svg><path></path></svg></span> and EN 14214 specifications. Increasing the bicarbonate concentration to 100 mM and employing a fed-batch mode elevated the biomass to 1.45 ± 0.32 g/L, carbohydrate accumulation to 58.25 ± 1.28 % DCW, and carbon fixation efficiency to 63.8 ± 3.23 %, which is significantly higher than that of the control cultivated with CO₂ supply (0.30 ± 0.04 %). This study highlights the potential of bicarbonate ion supplementation as an effective strategy for maximal biomass production with high productivity and biomolecule accumulation, aiding in the realization of cost-effective microalgal biofuel production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104064"},"PeriodicalIF":4.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}