Algal Research-Biomass Biofuels and Bioproducts最新文献

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Three non-invasive tools to estimate microalgal growth online and in real-time 三种非侵入性工具在线和实时评估微藻生长
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-07-03 DOI: 10.1016/j.algal.2025.104183
Henrik Geltner , Sjoerd Kortstee , Maria Barbosa , Marcel Janssen
{"title":"Three non-invasive tools to estimate microalgal growth online and in real-time","authors":"Henrik Geltner ,&nbsp;Sjoerd Kortstee ,&nbsp;Maria Barbosa ,&nbsp;Marcel Janssen","doi":"10.1016/j.algal.2025.104183","DOIUrl":"10.1016/j.algal.2025.104183","url":null,"abstract":"<div><div>On-line monitoring of bioprocesses is a cumbersome task that is relentlessly needed for accurate control and maximization of process efficiency, productivity, and yields. For microalgal processes, developing proper monitoring systems can be especially challenging due to their diverse characteristics. In this study, a novel approach is presented for non-invasively estimating microalgal growth in batch processes by online monitoring of the required nitric acid supply for pH control based on the proton stoichiometric imbalance of nitrate metabolism. Employing this strategy, we further increase measurement redundancy and estimate reliability by integrating gas analysis of carbon dioxide and oxygen fractions in the off-gas to determine biomass growth on-line. The obtained estimates of biomass concentrations were tested and validated in three autotrophic batch cultivations of <em>Tetradesmus obliquus</em> at a laboratory scale with a working volume of 1.8 L. All three online monitoring approaches could be employed for biomass estimation with outstanding results. Compared to offline measurements of biomass dry weight, correlation coefficients (R<sup>2</sup>) of 0.96, 0.94, and 0.96 were achieved for the three monitoring methods. Differences in measurements of cell dry weight were determined to lie within 8 %, 7.1 %, and 6.7 % for normalized root mean square deviations (N-RMSD). The presented method of mass-balance-based growth determination proved suitable for real-time monitoring of microalgal biomass production. We believe this work paves the way for further process automation of the microalgal output by expanding on-line monitoring capabilities for biomass and cell state in parallel.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104183"},"PeriodicalIF":4.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microalgae-based meat analogs: A review on types, sustainable production, challenges, and prospects 基于微藻的肉类类似物:类型、可持续生产、挑战和前景综述
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-07-03 DOI: 10.1016/j.algal.2025.104180
Ramesh Sharma , Sajesh Chettri , Uttara Mahapatra , Krishnakumar Kannan , Tarun Kanti Bandyopadhyay , Biswanath Bhunia
{"title":"Microalgae-based meat analogs: A review on types, sustainable production, challenges, and prospects","authors":"Ramesh Sharma ,&nbsp;Sajesh Chettri ,&nbsp;Uttara Mahapatra ,&nbsp;Krishnakumar Kannan ,&nbsp;Tarun Kanti Bandyopadhyay ,&nbsp;Biswanath Bhunia","doi":"10.1016/j.algal.2025.104180","DOIUrl":"10.1016/j.algal.2025.104180","url":null,"abstract":"<div><div>Microalgae (especially <em>Spirulina</em> sp., and <em>Chlorella)</em> are a broad category of photosynthetic microorganisms with high protein content, quick growth rates, and little effect on the environment. The rising demand for sustainable and ethical food sources has driven innovation in alternative protein sources, with meat analogs being a focal point. This article discusses the processing of certain strains of microalgae to produce a meat substitute that closely resembles the nutritional value and sensory characteristics of real meat. This study explores the importance, properties, and potential of microalgae as a primary ingredient in the development of meat analogs. The review also discusses the estimation of protein quality and the role of bio-functional peptides, addressing the worldwide production of microalgae. Additionally, it addresses the potential manufacturing process with advanced food processing technologies such as extrusion and 3D printing, electrospinning, and cuvette shear cell to achieve the desired texture and appearance of meat analog. This review addresses the significant continuing research on the effects of extrusions and 3D printing on the nutritional, color, flavor, sensory, and textural qualities of microalgae-based meat analogs (MBMA). The final section discusses the significance of flavor modification, production difficulties, and prospects for MBMA. The study concludes that MBMA offers a promising alternative to conventional meat, with potential benefits including reduced environmental impact, improved nutritional value, and alignment with ethical food consumption trends. The manufacturing of meat analogs requires a challenging effort to preserve food safety, mainly in the preprocessing of algal biomass to identify the toxic and odorous compounds. Since the development of a highly textural meat analogue is still in its infancy, more studies must be done in the future. Future research will focus on scaling production, enhancing sensory attributes, and exploring the commercial viability of microalgae meat analogues.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104180"},"PeriodicalIF":4.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial activity of North Atlantic Ocean macroalgae and the size-activity relationships of phlorotannins in Fucus vesiculosus 北大西洋大型藻类的抑菌活性及墨角藻中褐藻单宁的大小-活性关系
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-07-03 DOI: 10.1016/j.algal.2025.104184
Adrian H. Kopf , Anna Hammer , René A.M. Dirks , Carla Araya-Cloutier
{"title":"Antimicrobial activity of North Atlantic Ocean macroalgae and the size-activity relationships of phlorotannins in Fucus vesiculosus","authors":"Adrian H. Kopf ,&nbsp;Anna Hammer ,&nbsp;René A.M. Dirks ,&nbsp;Carla Araya-Cloutier","doi":"10.1016/j.algal.2025.104184","DOIUrl":"10.1016/j.algal.2025.104184","url":null,"abstract":"<div><div>Macroalgae (seaweeds) are utilized in the feed and food industries, with certain species recognized for their bioactive properties such as antimicrobial activity. Fourteen species of edible macroalgae harvested in the North Atlantic Ocean were screened for antimicrobial activity followed by tentative compound annotation using liquid chromatography and mass spectrometry. Eighty-five crude extracts were obtained via ultrasound assisted sequential solvent extraction. Methanol extracts of several brown macroalgae (Phaeophyceae) showed potential for valorization due to their high yield of crude material (~7 %) and antibacterial activity against the foodborne pathogen <em>Bacillus cereus</em> (zones of inhibition 4–8 mm). The crude methanolic extract of <em>Fucus vesiculosus</em> showed the highest activity with a minimum inhibitory concentration (MIC) of 1 mg/mL. Chemical analyses revealed the presence of phlorotannins as one of the main secondary metabolites and phlorotannin content correlated well with the found antimicrobial activities. Through flash chromatographic fractionation, phlorotannin-enriched pools were obtained with increased potency (MIC 0.25 mg/mL). It was found that the antimicrobial activity was dependent on the molecular size of the phlorotannins, with an optimum degree of polymerization of around twenty (molecular mass ~2.5 kDa), highlighting that polymer length is important for the antimicrobial activity of phlorotannins against <em>B. cereus</em>. This study contributes to understanding the size-activity relationships of this promising class of natural products from valuable marine sources as antimicrobial agents.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104184"},"PeriodicalIF":4.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Profiling and antiproliferative activity of tropical seaweed extracts using LC-HRMS and in silico approaches 利用LC-HRMS和计算机方法分析热带海藻提取物及其抗增殖活性
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-07-02 DOI: 10.1016/j.algal.2025.104181
Fitri Budiyanto , Martha Sari , Sekar Ratri Widyaningrum , Agus Budiawan Naro Putra , Kartika Dyah Palupi , Fauzy Rachman , Ferbiana Untari , Tri Handayani , Abdullah Rasyid
{"title":"Profiling and antiproliferative activity of tropical seaweed extracts using LC-HRMS and in silico approaches","authors":"Fitri Budiyanto ,&nbsp;Martha Sari ,&nbsp;Sekar Ratri Widyaningrum ,&nbsp;Agus Budiawan Naro Putra ,&nbsp;Kartika Dyah Palupi ,&nbsp;Fauzy Rachman ,&nbsp;Ferbiana Untari ,&nbsp;Tri Handayani ,&nbsp;Abdullah Rasyid","doi":"10.1016/j.algal.2025.104181","DOIUrl":"10.1016/j.algal.2025.104181","url":null,"abstract":"<div><div>Seaweeds offer significant potential at the intersection of functional food development and drug discovery, as they can be safely incorporated into the human diet and exhibit antiproliferative activity against cancer cell lines. This study aims to investigate the metabolite profile of water extracts from seaweed and evaluate their antiproliferative properties. The methodology employed in this study encompassed six key steps: extraction, LC-HRMS-based metabolite identification, antiproliferative assays, correlation analysis using partial least squares regression (PLSR), molecular docking, and molecular dynamics (MD) simulations. Metabolites were extracted from three seaweed species via maceration using deionized water, followed by profiling through liquid chromatography–high-resolution mass spectrometry (LC-HRMS). Among the extracts, <em>Sargassum cinereum</em> demonstrated the most potent antiproliferative activity, with an IC₅₀ value of 46.89 μg/mL as determined by the MTT assay. PLSR analysis identified meconic acid as a key predictor of cell viability. In the in silico analysis, docking scores obtained from AutoDock Vina were re-ranked using the XGBoost machine learning algorithm. The top-ranked compounds based on binding affinity to the target protein (PDB ID: <span><span>7KP8</span><svg><path></path></svg></span>) were (3β,22E,24S)-3-Hydroxyergosta-5,8,22-trien-7-one from <em>Ulva lactuca</em> (−10.02 kcal/mol), 2,3,5,6,8-pentahydroxy-1,4-naphthalenedione from <em>S. cinereum</em> (−8.40 kcal/mol), and 5,2′,5′-trihydroxy-3,7,8-trimethoxyflavone 2′-acetate from <em>Turbinaria decurrens</em> (−8.80 kcal/mol). Molecular dynamics simulations over 100 ns confirmed the stability of these ligand–protein complexes. These findings indicate that metabolites derived from <em>U. lactuca</em>, <em>S. cinereum</em>, and <em>T. decurrens</em> possess promising potential compounds with inhibitory activity against lung cancer progression.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104181"},"PeriodicalIF":4.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photoperiod strategies to increase microalgae-pigment content: A new level-up to natural food colorants production 提高微藻色素含量的光周期策略:天然食用色素生产的新水平
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-07-02 DOI: 10.1016/j.algal.2025.104185
Tatiele Casagrande do Nascimento , Pricila Nass Pinheiro , Mariany Costa Deprá , Rafaela Basso Sartori , Maria Roca , Eduardo Jacob-Lopes , Leila Queiroz Zepka
{"title":"Photoperiod strategies to increase microalgae-pigment content: A new level-up to natural food colorants production","authors":"Tatiele Casagrande do Nascimento ,&nbsp;Pricila Nass Pinheiro ,&nbsp;Mariany Costa Deprá ,&nbsp;Rafaela Basso Sartori ,&nbsp;Maria Roca ,&nbsp;Eduardo Jacob-Lopes ,&nbsp;Leila Queiroz Zepka","doi":"10.1016/j.algal.2025.104185","DOIUrl":"10.1016/j.algal.2025.104185","url":null,"abstract":"<div><div>This study investigates the influence of different photoperiods on the pigment profiles of the microalgae <em>Scenedesmus obliquus</em>, focusing on maximizing the production of carotenoids and chlorophylls. Experiments were conducted under three photoperiod regimes: (i) Long-term (h:h) – light:dark cycles of 10:14, 12:12, 18:6, 20:4, 22:2, and 24:0 h; (ii) Frequencies (t/d): based on a previously defined optimal light/dark cycle of 22:2 h – in this setup, the 2 h of darkness were subdivided into 2, 4, 8, 12, 24, and 48 times per day, resulting in increasingly frequent light/dark transitions; and (iii) Short-term (s:s) – light:dark cycles in on second with ratios of 0.91:0.09, 0.83:0.17, 0.75:0.25, and 0.50:0.50. The short-term photoperiod of 0.91:0.09 proved to be the most effective condition for the overall maximization of pigments, standing out especially for the accumulation of all-<em>trans</em>-lutein (7.14 g/kg), and chlorophyll <em>a</em> (46.32 g/kg). In contrast, long-term photoperiods favored the accumulation of chlorophyll <em>b’</em>, and pheophytin <em>b</em> (12:12 h), 9-<em>cis</em>-violaxanthin (18:6 h), and antheraxanthin (20:4 h). On the other hand, the application of frequencies proved to be, in general, an unfavorable strategy for the total accumulation of pigments. However, productivity metrics revealed that the 4 t/d frequency was the most efficient for volumetric biomass production (3.85 mg/L), while the 0.91:0.09 h photoperiod provided the best results for carotenoid (31.84 mg/L) and total chlorophyll (165.11 mg/L). Finally, it is suggested that specific adjustments to the lighting regime can optimize the accumulation and productivity of natural pigments for industrial application, especially in conditions of very rapid light:dark alternations.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104185"},"PeriodicalIF":4.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insights into the molecular and morphological diversity of Asparagopsis taxiformis in north-eastern Australia 澳大利亚东北部taxxiformis天冬酰胺分子和形态多样性的研究
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-07-02 DOI: 10.1016/j.algal.2025.104182
Anna Wilson , Jessica E. Harris , Rocky de Nys , Dean R. Jerry
{"title":"Insights into the molecular and morphological diversity of Asparagopsis taxiformis in north-eastern Australia","authors":"Anna Wilson ,&nbsp;Jessica E. Harris ,&nbsp;Rocky de Nys ,&nbsp;Dean R. Jerry","doi":"10.1016/j.algal.2025.104182","DOIUrl":"10.1016/j.algal.2025.104182","url":null,"abstract":"<div><div>The red seaweed, <em>Asparagopsis taxiformis</em>, consists of multiple genetic lineages that have spread globally through natural and human-mediated dispersal. Endemic to Australia and the Indo-Pacific, <em>A. taxiformis</em> has become an invasive pest in regions such as the Mediterranean Sea. Despite the commercial and ecological importance of this species, the genetic and morphological diversity of Australian populations remains understudied. Understanding this diversity, particularly within biodiversity hotspots like the Great Barrier Reef (GBR), is essential for advancing ecological knowledge and improving conservation and commercial management. This study investigated the genetic and morphological diversity of <em>A. taxiformis</em> within north-eastern Australia, by collecting tetrasporophyte specimens from 13 near and offshore locations in Queensland, including the GBR Marine Park. DNA was extracted from specimens and the chloroplast RuBisCO spacer and mitochondrial <em>cox2-3</em> intergenic spacer (<em>cox2-3)</em> were sequenced to assess genetic diversity. Nine morphological characteristics of the tetrasporophyte were also measured. While little genetic variation was found using the RuBisCO spacer, <em>cox2-3</em> analysis revealed that lineage 5 (L5) was present at two locations, lineage 6 (L6) dominated southern Queensland and lineage 4 (L4) was prevalent in the northern GBR, marking its first record in Australian coastal waters. Morphometric analyses identified significant differences in axial cell width and filament width among L4 and L5, with these traits modelled to be most useful for differentiating between lineages. The findings highlight substantial genetic and morphological diversity within <em>A. taxiformis</em> populations of Australia and the need for further systematic sampling in underrepresented locations.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104182"},"PeriodicalIF":4.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Upcycling algal biomass into biodegradable materials: Enhancing structural properties and performance through hydrophobic modifications 将藻类生物质升级为可生物降解材料:通过疏水修饰增强结构特性和性能
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-07-01 DOI: 10.1016/j.algal.2025.104175
Yuchen Yang , Chujie Qi , Runhao Zhang , Yongze Li , Ganpei Tian , Li Gao , Bo Bian
{"title":"Upcycling algal biomass into biodegradable materials: Enhancing structural properties and performance through hydrophobic modifications","authors":"Yuchen Yang ,&nbsp;Chujie Qi ,&nbsp;Runhao Zhang ,&nbsp;Yongze Li ,&nbsp;Ganpei Tian ,&nbsp;Li Gao ,&nbsp;Bo Bian","doi":"10.1016/j.algal.2025.104175","DOIUrl":"10.1016/j.algal.2025.104175","url":null,"abstract":"<div><div>This study presents a sustainable approach for valorizing algal residual biomass (ARB) through its incorporation into PLA/PBAT bioplastic blends. The acetylated ARB demonstrated multifunctional properties, serving as both a nucleating agent (increasing relative crystallinity from 12.77 % to 32.30 % in PLA/PBAT 30/70 blends) and a compatibilizer (high-temperature tanδ peak vanished in PLA/PBAT 70/30 blends). Mechanical testing revealed ARB's unique plasticizing effect, particularly in PLA/PBAT (30/70) blends where it induced yield behavior and plastic deformation, increasing elongation at break from 7.24 % to 7.97 % with 25 % ARB content. The optimal 20 % ARB formulation achieved a balanced tensile strength of 6.24 MPa with 7.82 % elongation, while flexural modulus decreased from 1106.92 MPa to 200 MPa in PLA-rich blends, indicating significant system softening. Thermal analysis showed enhanced stability and improved crystallization behavior. The composites maintained excellent biodegradability (17.71 %/8 weeks) with no phytotoxicity, while reducing material costs by 14.13 %. Comparative analysis positioned ARB composites as superior to starch-based bioplastics, suggesting their potential as sustainable alternatives in bio-based plastic formulations. This work provides a practical pathway for developing cost-effective, eco-friendly bio-composites with tunable mechanical properties through ARB incorporation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104175"},"PeriodicalIF":4.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Real-time online monitoring of large-scale tubular photobioreactors through gas-phase composition analysis 基于气相组成分析的大型管状光生物反应器实时在线监测
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-06-30 DOI: 10.1016/j.algal.2025.104172
Luca Buscaglia, Fred van den End, Marcel Janssen, Maria Barbosa
{"title":"Real-time online monitoring of large-scale tubular photobioreactors through gas-phase composition analysis","authors":"Luca Buscaglia,&nbsp;Fred van den End,&nbsp;Marcel Janssen,&nbsp;Maria Barbosa","doi":"10.1016/j.algal.2025.104172","DOIUrl":"10.1016/j.algal.2025.104172","url":null,"abstract":"<div><div>Cost efficiency restricts the potential of large-scale microalgae industries and can be tackled by adopting monitoring and control systems to optimize resource use and to improve biomass productivity. Gas-phase composition monitoring is an efficient monitoring strategy in lab-scale photobioreactors, but its application in large-scale systems, especially tubular photobioreactors, remains limited. In this study, we developed a gas-phase-based online monitoring strategy to study real-time microalgal growth and productivity in a pilot-scale tubular photobioreactor. A gas analyzer was employed to evaluate oxygen and carbon dioxide transfer rates with 1 min resolution. These measurements allowed us to monitor daily fluctuations of photosynthetic quotient, dry weight concentration, and biomass productivity at a high resolution. The dry weight estimated from gas measurements matched the offline data, and the comparison was used to assess the accuracy of the method. In contrast, the turbidity sensor underestimated dry weight concentration. All online and offline estimations provided similar biomass productivity with a maximum around 0.65 g·L<sup>−1</sup>d<sup>−1</sup>, although biofilm growth on the tubes caused deviation at the end of cultivation. The oxygen volume fraction measurement of an insertable in-line optical oxygen sensor and of the paramagnetic oxygen analyzer were comparable, resulting in an average absolute error of 0.03%<em>v</em>/v. With this work, we showed that online process monitoring is useful to study microalgal growth and productivity in large-scale tubular photobioreactors. Nitrogen limitation was detected, and the photosynthetic quotient was quantified and employed to monitor the metabolic state of the culture.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104172"},"PeriodicalIF":4.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Web application for automated counting and size analysis of industrial microalgae monocultures using image processing and Convolutional Neural Network based deep learning 使用图像处理和基于卷积神经网络的深度学习的工业微藻单一栽培自动计数和大小分析的Web应用程序
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-06-27 DOI: 10.1016/j.algal.2025.104173
Elena-Roxana Popescu , Aslak Einbu , Linn Israelsen , Gunilla Eriksen , Nina Hardy , Richard A. Ingebrigtsen , Torbjørn Pettersen
{"title":"Web application for automated counting and size analysis of industrial microalgae monocultures using image processing and Convolutional Neural Network based deep learning","authors":"Elena-Roxana Popescu ,&nbsp;Aslak Einbu ,&nbsp;Linn Israelsen ,&nbsp;Gunilla Eriksen ,&nbsp;Nina Hardy ,&nbsp;Richard A. Ingebrigtsen ,&nbsp;Torbjørn Pettersen","doi":"10.1016/j.algal.2025.104173","DOIUrl":"10.1016/j.algal.2025.104173","url":null,"abstract":"<div><div>Accurate monitoring of microalgae cultures is crucial for long-term research on monocultures as well as for industrial scale-up processes, where maintaining culture quality directly impacts the consistency and success of the final product. Traditional microscope-based counting and sizing is labor-intensive and time-consuming, necessitating automated solutions, while lowering costs. This paper presents a web-based application, Algalytics, that integrates image processing techniques with a Convolutional Neural Network (CNN), to extract algae counts and size measurements from input images. The methodology involves image segmentation, classification into different categories which helps ensure accurate counting and sizing while avoiding the inclusion of impurities, followed by post-processing to extract measurements. The model is trained on labeled datasets, and after fine-tuning, it is deployed in a web-based application. Validation was performed on datasets featuring <em>Porosira glacialis</em> (a large centric diatom for CO<sub>2</sub> sequestration) and <em>Tisochrysis lutea</em> (a haptophyte often used for shellfish feeding). The approach was validated on datasets with varying image quality and algae density, demonstrating the robustness of the application. Results showed high counting accuracy, while sizing was more challenging due to optical artifacts such as halo effects, which introduced measurement biases. These challenges can be mitigated by improving image focus and acquisition settings. Despite this, the system effectively captures growth trends and provides a low-cost, user-friendly, and scalable alternative to manual methods, offering consistent and efficient monitoring for both industrial operation, and small research laboratories.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104173"},"PeriodicalIF":4.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Postharvest processing of Undaria pinnatifida: Effect of blanching and salting parameters on the quality of yudoshi-enzo wakame 裙带菜采后加工:焯水和腌制参数对优氏隐佐裙带菜品质的影响
IF 4.6 2区 生物学
Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-06-27 DOI: 10.1016/j.algal.2025.104178
Valeria Solana , Vanesa Ixtaina , Fernando Gaspar Dellatorre
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