Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"From spores to gametes: A sexual life cycle in a symbiotic Trebouxia microalga","authors":"Ayelén Gazquez ,&nbsp;César Daniel Bordenave ,&nbsp;Javier Montero-Pau ,&nbsp;Marta Pérez-Rodrigo ,&nbsp;Francisco Marco ,&nbsp;Fernando Martínez-Alberola ,&nbsp;Lucia Muggia ,&nbsp;Eva Barreno ,&nbsp;Pedro Carrasco","doi":"10.1016/j.algal.2024.103744","DOIUrl":"10.1016/j.algal.2024.103744","url":null,"abstract":"<div><div>Trebouxiophyceae are particularly widespread in terrestrial environments and comprise most of the lichen-forming microalgae genera. These symbionts have been frequently considered asexual, however, their life cycles remain largely unknown. We sequenced and analysed the nuclear genome of <em>Trebouxia lynniae</em>, a model Trebouxiophyceae phycobiont, monitored and modelled its colony proliferation and analysed cell population dynamics by using flow cytometry coupled with microscopy and ploidy analysis. The genome inspection unveiled the presence of a “meiosis toolkit”, indicative of sexual reproduction, and the absence of TALE transcription factors related with haplontic life cycles. Moreover, we reveal that <em>T. lynniae</em> possesses a diploid genome, sexual reproduction, and diplontic life cycle. Also, we have demonstrated that its zoospores are gametes, and that meiosis is prezygotic. These discoveries illuminate Trebouxiophyceae ecology and evolution, highlighting the potential adaptive significance of sex in the face of challenging and changing ecological conditions like those faced by lichen symbionts. Moreover, characterizing this terrestrial Chlorophyta's life cycle contributes to shape evolutionary theories that aim to elucidate the path that they took during terrestrialization, suggesting that, as proposed for Streptophyta, it may have been mediated by a life cycle shift.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103744"},"PeriodicalIF":4.6,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Target of Rapamycin (TOR) activity in the diatom Phaeodactylum tricornutum using commercially available materials","authors":"Yoshinori Tsuji ,&nbsp;Takumi Ishikawa","doi":"10.1016/j.algal.2024.103746","DOIUrl":"10.1016/j.algal.2024.103746","url":null,"abstract":"<div><div>Target of rapamycin (TOR) is a conserved protein kinase that regulates the balance between catabolic and anabolic processes in response to nutrient availability. Although the central role of TOR kinase in nutrient stress responses is well-recognized, little is known about the molecular basis of TOR signaling in ecologically important secondary algae with plastids of red algal origin, such as diatoms, as assessing <em>in vivo</em> TOR kinase activity is a difficult task. To assess TOR kinase activity, the phosphorylation status of downstream components, such as ribosomal protein S6 (RPS6), must be measured. Unlike for model organisms, an antibody that detects phosphorylated (P-) RPS6 in diatoms is not commercially available. Therefore, we developed a convenient method in which P-RPS6 and non-P-RPS6 were detected via Phos-tag affinity electrophoresis and immunoblotting with a commercial antibody that cross-reacts with RPS6 (both P- and non-P-RPS6) in the diatom, <em>Phaeodactylum tricornutum</em>. Using this Phos-tag-based method, we observed a dose-dependent decrease in the P-RPS6/total RPS6 ratio in <em>P. tricornutum</em> cells treated with the TOR kinase inhibitor, AZD-8055. We also observed a reduction in the P-RPS6/total RPS6 ratio during the nitrogen-deficient culture of <em>P. tricornutum</em>, which indicated the inactivation of TOR kinase in response to nitrogen deficiency. Finally, we demonstrated the potential application of the Phos-tag-based method to other ecologically, evolutionarily, and industrially important secondary algae, such as <em>Nannochloropsis oceanica</em> (Stramenopiles), the haptophyte <em>Tisochrysis lutea</em>, and <em>Euglena gracilis</em> (Euglenid). As all experimental materials are commercially available, the Phos-tag-based method can be used to promote studies on TOR in diverse algae in different contexts.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103746"},"PeriodicalIF":4.6,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light and temperature culture conditions impact the metabolome of the cyanobacterium of therapeutic interest Planktothricoides raciborskii PMC 877.14","authors":"Inés Seijo Touceda ,&nbsp;Justine Demay ,&nbsp;Charlotte Duval ,&nbsp;Claude Yéprémian ,&nbsp;Anita Reinhardt ,&nbsp;Benjamin Marie","doi":"10.1016/j.algal.2024.103738","DOIUrl":"10.1016/j.algal.2024.103738","url":null,"abstract":"<div><div>Cyanobacteria are ancient photosynthetic microorganisms with a long evolutionary history that have adapted to inhabit diverse environments, such as thermal waters and muds. To do so, they are known to produce a wide range of bioactive molecules likely involved in adaptative traits such as high light and/or high temperature resistance mechanisms, which makes them particularly interesting for inclusion in thermal treatments and various therapeutic applications.</div><div>In this study, the impact of higher temperature and light intensity culture conditions on the metabolome of a promising cyanobacterial strain isolated from the muds of the Balaruc-les-Bains thermal station, <em>Planktothricoides raciborskii</em> PMC 877.14<em>,</em> was investigated through liquid chromatography and mass spectrometry analyses. Statistical analyses of the biological data and molecular network construction allowed for the exploration of potential metabolite induction under different culture conditions, in order to drive the production of specific metabolites aimed at coping with cellular stress. A global shift on intracellular metabolic composition was observed over time with increased biomass production. Higher light intensity was found to stimulate both growth and production of antioxidant and/or photoprotective molecules (such as ergothioneine, mycosporine-like amino acids or carotenoids) while an increase in temperature influenced the higher production of phycobilins (such as phycoerythrin and phycocyanin).</div><div>These results highlight the importance of culture/environmental conditions in driving intracellular metabolite differentiation and open up new perspectives in selecting of the optimal parameters for the growth and production of bioactive molecules for therapeutic applications in the cyanobacterium <em>P. raciborskii</em> PMC 877.14.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103738"},"PeriodicalIF":4.6,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the effects of two DNA methyltransferase inhibitors on DNA methylation and carotenoid metabolism in Chlamydomonas reinhardtii by comparative transcriptome and physiological analysis","authors":"Jv-Liang Dai ,&nbsp;Hao-Hong Chen ,&nbsp;Ming-Hua Liang ,&nbsp;Jian-Guo Jiang","doi":"10.1016/j.algal.2024.103739","DOIUrl":"10.1016/j.algal.2024.103739","url":null,"abstract":"<div><div>DNA methylation plays an important role in cell growth and development. However, little is known about the possible functions of DNA methylation in microalgae. Here, two DNA methyltransferase (DNMT) inhibitors (5-azacytidine and zebularine) were used to treat <em>Chlamydomonas reinhardtii</em> to investigate the effects of DNA methylation on microalgae. 5-Azacytidine (5-Azac) showed the promoting effects on cell growth and was benefit to the accumulation of pigments, while zebularine (Zeb) has adverse effects on the physiology of algal cells. However, the genomes of <em>C. reinhardtii</em> treated with two DNMT inhibitors unexpectedly showed slightly increased 5-mC levels. According to the results of transcriptome sequencing to algal cells treated with Zeb and 5-Azac, two DNMT inhibitors were found to up-regulate the transcription levels of genes involved in the cytosine methylation pathway and down-regulate genes involved in the cytosine demethylation pathway, which may result in higher 5-mC levels in genome. The increased content of carotenoids by 5-Azac may be due to the up-regulated transcription levels of carotenogenic genes. In addition, the up-regulated transcription levels of carotenoid degradation-related genes may be responsible for the reduced carotenoid content by Zeb. Finally, a <em>carotenoid 9,10(9′,10′)-cleavage dioxygenase 1</em> (<em>CrCCD1</em>) gene was isolated from <em>C. reinhardtii</em>, and its important role in carotenoid degradation was demonstrated. In summary, this study revealed the physiological changes and molecular mechanisms of <em>C. reinhardtii</em> under the effects of two DNMT inhibitors, providing valuable information for subsequent studies on DNA methylation in microalgae.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103739"},"PeriodicalIF":4.6,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytotoxic effects of Perfluorooctane sulfonate on the physiological responses of the seaweed Gracilaria lemaneiformis","authors":"Zhiwei Liu ,&nbsp;Linqing Liu ,&nbsp;Yufeng Yang ,&nbsp;Weiqiu Liu ,&nbsp;Xian Sun","doi":"10.1016/j.algal.2024.103741","DOIUrl":"10.1016/j.algal.2024.103741","url":null,"abstract":"<div><div>Perfluorooctane sulfonate (PFOS) is a man-made persistent organic pollutant that is commonly found in coastal ecosystems. However, there is limited knowledge about its impact on seaweed. In this study, <em>Gracilaria lemaneiformis</em> were cultured in the incubators to examine growth, antioxidant system, soluble substances, amino acid metabolism, and Chlorophyll a fluorescence transient (OJIP) in response to PFOS exposure at concentrations ranging from 0 to 50.0 mg L⁻¹. The results showed that the specific growth rate decreased in a concentration-dependent manner after 6 days, with a significant decrease observed only at 50.0 mg L⁻¹ (<em>p</em> &lt; 0.05). However, the malondialdehyde contents remained stable across different PFOS concentrations, indicating the strong antioxidant ability to the oxidative stress induced by PFOS exposure. This may be due to the combined impact of enzymatic (e.g., catalase and peroxidase) and non-enzyme (e.g., carotenoid, phycoerythrin, and phycocyanin) antioxidants. Furthermore, analysis of amino acid metabolism after 6 days of exposure revealed that the accumulation of specific amino acids, such as arginine (Arg), citrulline (Cit), and ornithine (Orn), played a vital role in improving resistance to PFOS toxicity. Additionally, the electron transport after the reduction of plastoquinone A (Q_A⁻) was highly sensitive to PFOS exposure. Based on the JIP-test analysis, it was inferred that PFOS potentially hinders the transport of electrons by binding to the plastoquinone B (Q_B) site of protein D₁ in the PSII reaction center. These findings will provide valuable insights into the potential influence of PFOS on seaweed cultivation, ultimately contributing to the better utilization of seaweed sources.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103741"},"PeriodicalIF":4.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AP-cyan – A potential ascorbate probe for algal cultures","authors":"Soujanya Kuntam ,&nbsp;Tímea Tóth ,&nbsp;Wai Yin Yau ,&nbsp;Péter Horváth ,&nbsp;Ho Yu Au-Yeung ,&nbsp;Szilvia Z. Tóth","doi":"10.1016/j.algal.2024.103743","DOIUrl":"10.1016/j.algal.2024.103743","url":null,"abstract":"<div><div>Ascorbate (Asc) is an important antioxidant that also participates in various biological processes in plants such as hormone metabolism, stress response and signaling pathways. Asc is also a vital vitamin for human health and enriching its content through biofortification is a desirable objective. Therefore, reliable <em>in situ</em> methods for assessing Asc levels are essential. However, most of the existing fluorescent probes for Asc detection are limited to liquid samples, such as human sera or plant extract, or require sophisticated techniques and equipment for in-cell detection, such as photo-induced electron transfer or time-gated luminescence microscopy. Moreover, many of these probes are not cell wall permeable and cannot be used in plants or algal cells. In this article, we introduce a reaction-based, Asc probe – AP-cyan, that can efficiently and qualitatively detect Asc in various microalgal cultures, including <em>Chlamydomonas reinhardtii</em>, <em>Chlorella sorokianiana</em> and <em>Parachlorella kessleri</em>. The probe is simple to use and produces fast results that can be observed with standard fluorescence microscopes with basic blue, green and red filters. The probe has an emission range (λ_em = 488 nm) that does not overlap with chlorophyll autofluorescence, making it suitable for algal cells. Thus, our probe offers a simple and powerful method to detect Asc in microalgal cells.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103743"},"PeriodicalIF":4.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient screening of Chlorella vulgaris and C. variabilis using high-throughput biolog phenotype arrays","authors":"Chuchi Chen ,&nbsp;Steven M. Short ,&nbsp;Valerie C.A. Ward","doi":"10.1016/j.algal.2024.103740","DOIUrl":"10.1016/j.algal.2024.103740","url":null,"abstract":"<div><div>Microalgae, particularly <em>Chlorella</em> species, are versatile microorganisms with significant scientific potential in various domains, including recombinant protein production, wastewater treatment, biofuel production, bio-fertilizers, food source, pharmaceuticals, and carbon capture. However, conventional growth media have often been the default choice for <em>Chlorella</em> cultivation. This study utilizes Biolog phenotype array plates to explore the growth responses of <em>Chlorella vulgaris</em> and <em>Chlorella variabilis</em> to a broad spectrum of carbon, nitrogen, phosphorus, and sulfur sources. The growth dynamics were captured by integrating the area under the OD-time curve. The results revealed growth preferences for both <em>Chlorella</em> species, emphasizing their unique nutrient source requirements and illuminated some unexpected growth behaviors. <em>C. vulgaris</em> exhibited equal preference for trehalose as glucose, and <em>C. variabilis</em> was unable to metabolize nitrate or sucrose, two staples of modified BBM media most commonly used for its cultivation. These findings contribute to a deeper understanding of the metabolic capacities of <em>C. vulgaris</em> and <em>C. variabilis</em>, informing potentially more efficient and tailored microalgal cultivation practices across diverse applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103740"},"PeriodicalIF":4.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth and metabolism regulation of cinnamic acid and its derivatives to Auxenochlorella pyrenoidosa","authors":"Min Li ,&nbsp;Zhen-Fang Shang ,&nbsp;Zhi-Jing Ni,&nbsp;Hui-Jun Zhao,&nbsp;Qian Lei,&nbsp;Zhao-Jun Wei","doi":"10.1016/j.algal.2024.103742","DOIUrl":"10.1016/j.algal.2024.103742","url":null,"abstract":"<div><div><em>Auxenochlorella pyrenoidosa</em>'s industrial utility is closely associated with the concentration of high-value products. This study focuses on the effects of cinnamic acid (CA) and its derivatives, which known as secondary metabolites in higher plants and effective plant growth regulators, on the growth and metabolic profiles of <em>A. pyrenoidosa</em>. It was discovered that CA and its derivatives significantly enhance the biomass of <em>A. pyrenoidosa</em> under conditions of 10 mg/L over 48 h or 100 mg/L over 120 h. Cells treated with 10 mg/L CA exhibited lower oxidative stress and maintained robust metabolic activity. Metabolic pathways, including those for carbohydrates, lipids, small peptides, and cofactors, were stimulated, leading to the accumulation of a wide array of bio-products, notably high-value products such as α-linolenic acid, cis-4,7,10,13,16,19-docosahexaenoic acid (DHA), nicotinamide adenine dinucleotide plus hydrogen (NADH), glutathione, and vitamins B2/B3. This study demonstrates that CA and its derivatives are good nutritional enhancers for cultivating <em>A. pyrenoidosa</em>, highlighting their potential for broad application in biotechnological industries.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103742"},"PeriodicalIF":4.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing pigment production with cyanobacteria-rich microbiomes: Effect of light quality and exposure time","authors":"Marta Bellver ,&nbsp;Beatriz Altamira-Algarra ,&nbsp;Joan García ,&nbsp;Ivet Ferrer ,&nbsp;Eva Gonzalez-Flo","doi":"10.1016/j.algal.2024.103726","DOIUrl":"10.1016/j.algal.2024.103726","url":null,"abstract":"<div><div>Cyanobacteria-rich microbiomes may stand as a cost-effective source of pigments, as they exhibit improved stability in comparison to monocultures and do not require sterile media to grow. However, these bioproduct sources have not been thoroughly optimized for pigment production. The aim of the present study was to evaluate the effects of light quality and exposure time on pigment concentration and population distribution in 6 microbiomes rich in <em>Synechococcus</em> sp. and <em>Synechocystis</em> sp. A multivariable experimental design was implemented for selecting the optimal culture conditions in terms of light quality (white, red, green and blue) and exposure time (4 and 6 days). As a result, differential light quality exposures did not favor the growth of one species over another. Green light had a significantly positive effect on phycobiliprotein content in all microbiomes, with up to a 2.3-fold increase as compared to white light. Indeed, 4 days of exposure induced a change in phycobiliprotein composition in 5 microbiomes. Microbiome M11 was the best phycobiliprotein producer (content up to 145.5 mg gDW⁻¹). Meanwhile, carotenoid contents above 1.8 mg gDW⁻¹ were attained in all the microbiomes under optimized conditions. In conclusion, light quality modulation is a promising approach to stimulate the production of pigments in cyanobacteria-rich microbiomes.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103726"},"PeriodicalIF":4.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UV-B light (radiation) affects the metabolism of pigments and fatty acids in green algae Edaphochlorella mirabilis and Klebsormidium flaccidum in vitro","authors":"Giulia Santunione ,&nbsp;Francesca Masino ,&nbsp;Giuseppe Montevecchi ,&nbsp;Elisabetta Sgarbi","doi":"10.1016/j.algal.2024.103736","DOIUrl":"10.1016/j.algal.2024.103736","url":null,"abstract":"<div><div>Algae offer a rich source of bioactive compounds suitable for food products and bioenergy. Environmental challenges such as nutrient scarcity, extreme pH and temperature, high light intensity, and UV radiation usually trigger algae to produce excess of lipids, antioxidants, and other bioprotective molecules as part of their adaptations for survival. Algal cultivation provides proteins, lipids, carbohydrates, vitamins, antioxidants, and trace elements. This study focused on understanding how UV-B irradiance, as an abiotic stressor, can influence the growth and metabolite production of two green algal species, <em>Edaphochlorella mirabilis</em> (Chlorophyta) and <em>Klebsormidium flaccidum</em> (Charophyta). Using a temporary immersion system bioreactor for <em>in-vitro</em> algal growth, the results showed no significant difference in biomass for both algal species after the exposure to UV-B rays. However, the assessment of malondialdehyde levels revealed a significantly higher tendency towards lipid peroxidation in treated <em>E. mirabilis</em> (+ 90 %) compared to control. Conversely, <em>K. flaccidum</em> did not display significant differences, thereby highlighting a more advanced adaptive capacity against UV-B radiation<em>.</em> Overall, both algal species treated with UV-B showed increased pigment accumulation. <em>K. flaccidum</em> exhibited an average pigment increase of over 53 %, while <em>E. mirabilis</em> showed a lower increase, over 30 % on average. The notable rise in antioxidant compounds (lutein, β-carotene, and chlorophyll <em>a</em>) in UV-B exposed <em>K. flaccidum</em> samples also suggested a more suitable adaptive strategy to mitigate oxidative stress in Charophyta. In <em>K. flaccidum</em>, the increase in polyunsaturated fatty acids can be associated with increased production of antioxidant compounds. Conversely, <em>E. mirabilis</em> appeared to protect itself by decreasing polyunsaturated fatty acids in favor of saturated ones. In both algal species, the increase in secondary metabolites under UV stress highlighted potential as a novel food source for human consumption, deserving further investigation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103736"},"PeriodicalIF":4.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}