Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Characterization of allelochemicals from Nitzschia closterium and their allelopathic effects on Prorocentrum donghaiense","authors":"Hani Abdo ,&nbsp;Liju Tan ,&nbsp;Shuhui Wang ,&nbsp;Hongyan Xu ,&nbsp;Bingbing Wang ,&nbsp;Jiangtao Wang","doi":"10.1016/j.algal.2025.104085","DOIUrl":"10.1016/j.algal.2025.104085","url":null,"abstract":"<div><div>This study explores the allelopathic interactions and chemical characterization of the diatom <em>Nitzschia closterium</em> and its impact on the harmful dinoflagellate <em>Prorocentrum donghaiense</em>. Using mixed culture and filtrate culture experiments, the allelochemicals released by <em>N. closterium</em> were shown to inhibit <em>P. donghaiense</em> growth in a concentration-dependent manner. These inhibitory effects were linked to disruptions in photosystem II efficiency. In addition, extracts from liquid-liquid extraction (LLE) including solvent of ethyl acetate, n-hexane, petroleum ether, dichloromethane and DMSO, and solid phase extraction (SPE) of filtrate of <em>N. closterium</em> cultures, were tested for their effects on <em>P. donghaiense</em>. Among these, only the n-hexane extracts from LLE significantly reduced algal growth, achieving a 78.1 % inhibition at the highest concentration (50 μL). Fractionation of the n-hexane extracts via HPLC yielded twelve fractions, with fraction F-IV showing the most potent inhibition. Further GC–MS analysis identified several compounds, especially of 3-acetoxy-7,8-epoxylanostan-11-ol, as a key allelopathic agent. These findings contribute to understand the ecological role of allelopathy in regulating harmful algal blooms and highlight the potential of <em>N. closterium</em> extracts for environmental management application.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104085"},"PeriodicalIF":4.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whether controlling hydrodynamic conditions is an effective solution to alleviate cyanobacterial blooms","authors":"Peng Gu ,&nbsp;Wanqing Zhang ,&nbsp;Lianghao Xu ,&nbsp;Jun Ni ,&nbsp;Yi Ding ,&nbsp;Zengshuai Zhang ,&nbsp;Kunlun Yang ,&nbsp;Xueli Ren ,&nbsp;Hengfeng Miao","doi":"10.1016/j.algal.2025.104083","DOIUrl":"10.1016/j.algal.2025.104083","url":null,"abstract":"<div><div>The main purpose of this study was to determine the threshold and mechanism of the fluid field affecting cyanobacterial blooms based on monitoring of Taihu Lake fluid field data combined with artificial simulation experiments. The increase of carbon dioxide (CO₂) content in L-wave (wind speed of 3 m s⁻¹, wave height of 0.03 m) under simulated low wind waves promoted the peak of cell density, chlorophyll-a and optimal/maximal quantum yield of PSII (Fv/fm). At the same time, the water flow of 0.004–0.008 m s⁻¹ increased the collision frequency between cyanobacteria cells and activated the activity of antioxidant enzymes, so that cyanobacteria in the L-wave secreted more extracellular polymers (EPS) and α-<span>d</span>-glucopyranose, thereby enhancing cell adhesion. The maximum cell size of cyanobacteria was observed under the condition of L-wave strongly indicated that the low wind and wave environment promoted the formation of cyanobacterial blooms. However, the fluid field intensity was further enhanced to simulate the high-wave (H-wave, i.e., wind speed 3 m s⁻¹ and wave height 0.1 m). The excessive shear stress (4.54 × 10⁻² Pa) under H-wave produced a large amount of reactive oxygen species (ROS), which caused oxidative stress damage to cyanobacteria. The expression of peroxiredoxin (<em>prx</em>) increased by 1.57 times, which fully confirmed the imbalance of ROS scavenging system in cyanobacteria cells in H-wave, thereby inhibiting the formation of cyanobacterial blooms. By combining the monitoring and simulation experiments of Taihu Lake and using the structural equation model, it was found that adjusting the intensity of fluid field, colony formation and biomass were effective strategies to alleviate the outbreak of cyanobacterial blooms.</div></div><div><h3>Novelty and relevance statement</h3><div>The optimal fluid field thresholds and mechanism for bloom formation were determined through a combination of field monitoring and artificial simulation. However, exceeding this threshold (wind speed 3 m s⁻¹ and wave height 0.03 m) inhibited the formation of cyanobacterial blooms. This study will provide new insights and guidance for bloom control.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104083"},"PeriodicalIF":4.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nannochloropsis for the bioremediation of brewery side streams and co-production of aquaculture feed and bio-Fertiliser: A comprehensive review","authors":"Felix J. Brooke ,&nbsp;Qinge Ma ,&nbsp;Mohammadhosein Rahimi ,&nbsp;Alla Silkina ,&nbsp;Carmen Constantin ,&nbsp;Liliana Bădulescu ,&nbsp;Antonietta La Terza ,&nbsp;Burcin Atilgan Turkmen ,&nbsp;Aadil Bajoub ,&nbsp;El Amine Ajal ,&nbsp;Danny van Mullem ,&nbsp;Ronald Halim","doi":"10.1016/j.algal.2025.104079","DOIUrl":"10.1016/j.algal.2025.104079","url":null,"abstract":"<div><div>The brewing industry produces a large amount nutrient-rich wastewater. This review proposes a framework that harnesses autotrophic microalgae to establish a circular brewing industry. The study focuses on <em>Nannochloropsis</em> due to their high omega-3 polyunsaturated lipids and protein content, enabling the upcycling of brewery wastewater into aquaculture feed and biofertiliser. In the proposed system, <em>Nannochloropsis</em> is cultivated on brewery wastewater under mixotrophic conditions, and the resultant biomass is used as a fishmeal replacement or biofertiliser. This approach reduces chemical and energy demands for brewery wastewater treatment, while also alleviating aquaculture's reliance on fishmeal derived from unsustainable wild-caught fishing and agriculture's dependence on carbon-intensive mineral fertilisers. A summary of the research to-date on the cultivation of microalgae on brewery wastewater, the use of brewer's spent grain as widely available and inexpensive carbon for microalgal fermentation, the application of <em>Nannochloropsis</em> in aquaculture and bio-fertiliser, and techno-economic and life-cycle assessments of the proposed system are provided. A mass balance of the system suggests that for every 1000 L of brewery wastewater that is treated, 1.0–1.4 kg of <em>Nannochloropsis</em> biomass can be produced, enabling the removal of 95–100 % of N and P and 60–90 % COD reduction from the wastewater and the direct capture of 0.7–1.3 kg of CO₂, while producing 0.4–0.8 kg of lipids for aquafeed formulations and 0.6–1.0 kg of biomass residue for biofertilisers. An integrated approach that combines laboratory research with pilot-scale validation and iterative techno-economic assessments is needed to inform optimisation and guide scale-up.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104079"},"PeriodicalIF":4.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking biochemical profile of Chaetoceros calcitrans, Cylindrotheca fusiformis, and Nannofrustulum shiloi (Bacillariophyta) for nutritional and added-value applications","authors":"Ana S.P. Moreira ,&nbsp;Helena R. Rocha ,&nbsp;Susana Aveiro ,&nbsp;Andreia S. Ferreira ,&nbsp;Kayane Oliveira ,&nbsp;Alexandra Conde ,&nbsp;Marta Coelho ,&nbsp;Alexandre M.C. Rodrigues ,&nbsp;Natacha Coelho ,&nbsp;Hugo Pereira ,&nbsp;Ana Gomes ,&nbsp;Manuela Pintado ,&nbsp;Cláudia Nunes ,&nbsp;Manuel A. Coimbra ,&nbsp;Sónia P.M. Ventura ,&nbsp;Rosário Domingues ,&nbsp;Diana Lopes","doi":"10.1016/j.algal.2025.104080","DOIUrl":"10.1016/j.algal.2025.104080","url":null,"abstract":"<div><div>Diatoms have garnered attention as sources of natural bioactive compounds, making them attractive for developing high-value products for different biotechnological ends, particularly for nutritional applications. Despite their potential, the chemical composition of industrially produced biomass remains largely underexplored. In this context, this study was focused on a thorough characterisation of the biochemical profile of three marine diatom species produced outdoors in photobioreactors: <em>Chaetoceros calcitrans</em>, <em>Cylindrotheca fusiformis</em>, and <em>Nannofrustulum shiloi</em>. The proximal composition of biomasses accounted for 29–35 % ashes, 21–30 % proteins, 7–14 % lipids, 3–7 % carbohydrates, and 0.4–2 % pigments. Each species contained &gt;35 % of essential amino acids, mainly alanine and leucine. Distinct soluble protein and peptide size distribution patterns were observed among the three species. Galactose (26–45 mol%), uronic acids (20–29 mol%), and glucose (3–21 mol%) were the main sugar residues found in all species. Galactose was mainly derived from floridoside and related compounds. The most abundant fatty acid was 20:5 <em>n</em>-3 in <em>C. calcitrans</em> (18 %), whereas in <em>C. fusiformis</em> was 16:1 <em>n</em>-7 (20 %) and 16:0 in <em>N. shiloi</em> (21 %). Lipidomics revealed 325 species of glycolipids, phospholipids, betaine lipids, sphingolipids, fatty amides, and sterol lipids, also with a distinct distribution along the lipid classes. <em>C. calcitrans</em> was the richest in chlorophylls (14 mg.g⁻¹) and carotenoids (8 mg.g⁻¹). In mineral analysis, calcium showed the highest difference, 66 mg.g⁻¹ in <em>N. shiloi</em>, 27 mg.g⁻¹ in <em>C. fusiformis</em>, and 4 mg.g⁻¹ in <em>C. calcitrans</em>. Overall, this study highlights the nutritional potential of industrially produced diatoms, with distinct chemical profile that can be explored for target applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104080"},"PeriodicalIF":4.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transporters at the root of organic carbon acquisition and metabolism in eukaryotic microalgae - current knowledge and engineering potential","authors":"Antonina Karakostova,&nbsp;Hussam Hassan Nour-Eldin","doi":"10.1016/j.algal.2025.104078","DOIUrl":"10.1016/j.algal.2025.104078","url":null,"abstract":"<div><div>Microalgae play a key role in the global carbon cycle both as primary producers of photosynthetic assimilates and also as consumers of reduced carbon sources which some species can use to sustain hetero- and mixotrophic growth. Acquisition of reduced carbon and the partitioning of intermediates derived from its metabolism between different intracellular compartments requires the function of membrane transporter proteins positioned at the plasma membrane and the membranes of various organelles. Despite their important role in controlling the strength and direction of carbon fluxes, relatively little is known about microalgal membrane transporters of organic carbon substrates. Here we review state-of-the-art knowledge on microalgal transporters involved in acquisition of reduced carbon sources and intracellular carbon partitioning. Furthermore, we explore how this knowledge can be applied in engineering of novel microalgal strains with improved properties for use in various biotechnological fields.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104078"},"PeriodicalIF":4.6,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction, purification and antioxidant activity of Chlorella polysaccharide","authors":"Bingfeng Zhou,&nbsp;Jiaming Jiang,&nbsp;Yu Huo,&nbsp;Juying Lin,&nbsp;Changhua Shang","doi":"10.1016/j.algal.2025.104077","DOIUrl":"10.1016/j.algal.2025.104077","url":null,"abstract":"<div><div>The antioxidant activity of <em>Chlorella</em> polysaccharide has broad application prospect in healthcare. Ultrasound, hot water, microwave, freeze-thaw, alkali and enzyme assisted extraction methods can achieve high extraction efficiency of polysaccharide by optimizing extraction conditions. However, these methods may have risks of polysaccharide degradation, changes in polysaccharide structure and biological activity due to too long time (such as 6 h) or too high temperature (such as 170 °C). Therefore, mild, green and efficient extraction conditions need to be further studied during optimization process. This study extracted polysaccharide from <em>Chlorella</em> using four traditional methods (alkali, ultrasound, repeated freeze-thaw and hot water) and their combination, and optimized extraction process for the method with the highest polysaccharide extraction rate (alkali/hot water) using response surface methodology (Central Composite Design, CCD). We compared protein removal efficiency of Sevage reagent, trichloroacetic acid (TCA) and salting out for polysaccharide extracted by alkali-hot water method (AHP). Three components including neutral polysaccharide (AHP-1) and acidic polysaccharide (AHP-2 and AHP-3), were isolated and purified by DEAE-52 cellulose column chromatography, and their antioxidant activities were investigated. The results showed that Sevage reagent had the best protein removal efficiency (94.6 %, <em>w</em>/w) for AHP. In addition, three polysaccharide components exhibited different DPPH and ABTS free radical scavenging activities and iron reducing abilities (AHP-2 &gt; AHP-3 &gt; AHP-1). AHP-2 has great potential for the development of natural antioxidants and may serve as a component of functional foods. In summary, this study obtained high extraction rate and purity of <em>Chlorella</em> polysaccharide AHP through extraction and purification, and AHP exhibited high antioxidant activities. The results indicate that AHP has great potential for the development of natural antioxidants, laying the foundation for its application in food, medicine and beauty industries.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104077"},"PeriodicalIF":4.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ monitoring of HCO3−, CO32− and NO3− dynamics during microalgae culture by UV resonance raman spectroscopy","authors":"Fei Zhang ,&nbsp;Xun Wang ,&nbsp;Yu Zhang ,&nbsp;Zhen Zhu ,&nbsp;Yiming Jiang ,&nbsp;Guoqing Jia ,&nbsp;Xupeng Cao ,&nbsp;Fengtao Fan ,&nbsp;Can Li","doi":"10.1016/j.algal.2025.104076","DOIUrl":"10.1016/j.algal.2025.104076","url":null,"abstract":"<div><div>Microalgae can convert inorganic carbon (CO₂) and nitrogen (NO_x) into biomass products. However, detection of the concentration of inorganic carbon or nitrogen sources under in-situ culture condition is difficult. In this work, taking <em>Dunaliella salina</em> as a model, the concentration of carbon and nitrogen source in microalgae culture systems are real-time and in-situ detected using UV resonance Raman spectroscopy directly without pretreatment. Due to the resonance Raman effect, the detection limit of inorganic components in the culture medium, HCO₃⁻, CO₃⁻ and NO₃⁻ can reach 10 mM, 2.5 mM, and 0.05 mM respectively. To further indicate the potential of UV Raman spectroscopy, the nitrogen concentration of microalgae culture is monitored using a UV Raman spectrometer at 450 m below the sea. These results demonstrate that the UV resonance Raman spectroscopy is a powerful technique for monitoring the carbon and nitrogen sources for microalgae growth.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104076"},"PeriodicalIF":4.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional insights into the chloroplast rps2 gene from Dunaliella salina: Enhanced cadmium adsorption and resistance in recombinant bacteria","authors":"Jia-Yuan Xiao ,&nbsp;Tian-Hong Chen ,&nbsp;Ming-Xu Wu ,&nbsp;Xiao-Yu Zeng ,&nbsp;Jia-Lang Zheng ,&nbsp;Qing-Ling Zhu","doi":"10.1016/j.algal.2025.104075","DOIUrl":"10.1016/j.algal.2025.104075","url":null,"abstract":"<div><div>Cadmium (Cd) is a toxic heavy metal that poses significant ecological and health risks. Marine microalgae, such as <em>Dunaliella salina</em>, exhibit remarkable Cd adsorption and tolerance, making them valuable models for studying Cd toxicity and bioremediation. This study identified the chloroplast 30S ribosomal protein gene (<em>rps2</em>) as the most Cd-sensitive gene in <em>D. salina</em>. At 10 μg/L Cd, chloroplast genome-encoded genes related to translation and photosynthesis were upregulated, with <em>rps2</em> displaying sustained upregulation, whereas at 100 μg/L Cd, these genes were downregulated, with <em>rps2</em> exhibiting continuous downregulation. <em>rps2</em> transcription increased at low Cd levels (2.5 and 25 μg/L) but declined at higher concentrations (250 and 2500 μg/L), while protein levels remained stable at low Cd levels (2.5, 25 and 250 μg/L) but dropped significantly at high levels (2500 μg/L). The recombinant strain exhibited enhanced Cd tolerance and adsorption, with higher intracellular Cd levels and reduced Cd in the supernatant compared to controls. The RPS2 protein was detected in both cells and supernatants. Molecular weight discrepancies (63 kDa in <em>D. salina</em> vs. 99 kDa in recombinant strains) suggest post-translational cleavage in <em>D. salina</em>. These findings underscore the critical role of <em>rps2</em> in Cd biosorption and tolerance, offering a promising approach for aquatic Cd bioremediation. Furthermore, this study provides a theoretical foundation for further exploring of the molecular mechanisms of the <em>rps2</em> gene in response to heavy metal stress, and highlights potential non-ribosomal functions and post-translational modifications of ribosomal proteins.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104075"},"PeriodicalIF":4.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-infrared spectroscopy for microalgae studies: A comprehensive review of applications and outlooks","authors":"Runyu Zheng,&nbsp;Mohammed Kamruzzaman","doi":"10.1016/j.algal.2025.104074","DOIUrl":"10.1016/j.algal.2025.104074","url":null,"abstract":"<div><div>Microalgae are photosynthetic organisms typically found in natural freshwater and marine environments. They are important in wastewater treatment for pollution removal and resource recovery. Due to species-specific differences in physiological structure and biochemical composition, microalgae exhibit complex internal metabolisms and dynamic interactions with external environmental factors. However, many of their traits such as components, elements, and genetic information, and key mechanisms including growth and development, metabolism, and toxicological responses, remain insufficiently understood. As an effective, efficient, accurate, and nondestructive optical method, near-infrared (NIR) spectroscopy has great potential for studying microalgal properties. Considering the lack of literature reviews in this field, this paper highlights NIR applications on microalgae from three major categories: components testing, growth monitoring, and contaminants supervision using microalgae as a mediating object. It also discusses the existing challenges and future directions from aspects of microalgae, NIR, predictive modeling, and sustainability. Overall, this review aims to provide a comprehensive overlook and promote further advances at the intersection of microalgae research and NIR spectroscopy.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104074"},"PeriodicalIF":4.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaling-up information from high-throughput pulsed light data to predict microalgae growth dynamics in photobioreactors","authors":"Alberto Saccardo ,&nbsp;Juliane Wolf ,&nbsp;Ben Hankamer ,&nbsp;Fabrizio Bezzo","doi":"10.1016/j.algal.2025.104073","DOIUrl":"10.1016/j.algal.2025.104073","url":null,"abstract":"<div><div>Single cell green algae (microalgae) have evolved to tap into the vast solar energy resource via photosynthesis to produce biomass, which is a feedstock for a broad array of products including foods, feeds, fuels, biomaterials and fine chemicals. Microalgae are therefore the chassis for an array of solar biotechnologies.</div><div>In this study, we reformulated a photosynthetic unit model to capture the effect of light-dark cycles induced by self-shading and mixing in scaled-up systems. We calibrated and validated our model using High-Throughput Screening (HTS) data for <em>Chlamydomonas reinhardtii</em> under pulsed and continuous light regimes. By reconstructing the light history of individual cells migrating through a cultivation system, we established a link between HTS data and scaled-up systems, and validated the approach using published cultivation data.</div><div>In a case study of an outdoor microalgae cultivation scenario we demonstrated how our model captures the dynamics of both incident solar irradiance and attenuated light intensity along a simulated mixing cycle. The analysis of internal model variables provided insights into the 24-hour biomass growth profile and biomass productivity of the system. Finally, we showed how the model can be exploited for the optimisation of cultivation systems, enabling the definition of optimal forecast illumination intensities, mixing cycles, inoculation densities, and light paths.</div><div>This work represents a first step in linking small-scale HTS platforms with large-scale cultivation systems (i.e. photobioreactors). This provides a foundation to accelerate and de-risk the scale-up of microalgae production systems towards industrialised algae farming and processing.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104073"},"PeriodicalIF":4.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}