Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"The metabolic protective effects and gut microbiota modulation of bioactive peptides derived from Spirulina platensis in diabetic mice","authors":"Lijin Jiang,&nbsp;Youcai Zhou,&nbsp;Pingying Liu,&nbsp;Yiping Zhu,&nbsp;Xinyu Zhang,&nbsp;Yajuan Chen,&nbsp;Yangming Wen,&nbsp;Bilian Chen,&nbsp;Yongjin He","doi":"10.1016/j.algal.2026.104797","DOIUrl":"10.1016/j.algal.2026.104797","url":null,"abstract":"<div><div>Diabetes is a metabolic disorder associated with hyperglycemia, dyslipidemia, organ injury, and gut microbiota dysbiosis. This study investigated whether <em>Spirulina platensis</em>-derived peptides (SPP, ≤5 kDa) could alleviate metabolic dysfunction in streptozotocin (STZ)-induced diabetic mice. The SPP fraction used for animal intervention was characterized by liquid chromatography-tandem mass spectrometry (LC-MS/MS), which identified multiple high-confidence short peptide sequences. Male ICR mice were rendered diabetic by a single STZ injection and then orally administered SPP at 100, 150, or 200 mg·kg⁻¹ for 4 weeks, with pioglitazone (PIO) as a positive control. SPP attenuated polyphagia, polydipsia, body-weight loss, hyperglycemia, dyslipidemia, and liver, spleen, and kidney injury, while increasing hepatic glycogen content and improving oral glucose tolerance. Western blotting showed that SPP increased hepatic IRS1 and p-AMPK (Thr172) levels while reducing GSK3β expression, suggesting improved insulin signaling and energy homeostasis. 16S rRNA gene sequencing revealed that STZ-induced diabetes reduced α-diversity and disrupted gut microbiota composition, whereas SPP, particularly at the highest dose, partially restored microbial diversity and enriched potentially beneficial taxa, including the <em>Eubacterium coprostanoligenes</em> group and <em>Candidatus Saccharimonas</em>. PICRUSt2 predictions further suggested that SPP was associated with changes in microbial pathways related to lipid metabolism, cofactor/vitamin biosynthesis, bacterial toxins, and bile acid metabolism. Collectively, these findings indicate that SPP may exert protective effects in STZ-induced diabetic mice partly associated with improved hepatic insulin signaling and altered gut microbiota composition, supporting its potential as a dietary strategy for alleviating diabetes-associated gut-liver dysfunction.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104797"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic approaches to enhance heme in microalgae and cyanobacteria: Toward sustainable nutrition and taste","authors":"Mina Motavallizadeh Ardakani,&nbsp;Damian W. Laird,&nbsp;Abha Chopra,&nbsp;Houda Ennaceri,&nbsp;Navid Reza Moheimani","doi":"10.1016/j.algal.2026.104823","DOIUrl":"10.1016/j.algal.2026.104823","url":null,"abstract":"<div><div>Heme is an iron-containing compound with important applications in food technology, nutrition, and medicine. In addition to contributing meat-like colour and flavour, heme can serve as a natural alternative to nitrite curing agents and as a highly bioavailable iron source for addressing iron-deficiency anaemia. Growing demand for sustainable heme production has increased interest in microalgae and cyanobacteria as alternative production platforms due to their rapid growth, photosynthetic efficiency, and natural capacity for tetrapyrrole biosynthesis. However, studies directly investigating heme accumulation and targeted pathway engineering in these organisms remain limited.</div><div>This review summarizes current knowledge of tetrapyrrole and heme biosynthesis in microalgae and cyanobacteria, with an emphasis on key enzymatic and regulatory steps influencing heme production. Potential engineering targets across the pathway are discussed. Because direct heme-focused studies in microalgae are still scarce, insights are partly informed by advances in engineering related metabolites and by metabolic engineering strategies established in bacteria and yeast.</div><div>Strain improvement approaches are discussed, including random mutagenesis and genetic engineering, together with current limitations related to genetic tools, pathway regulation, and large-scale cultivation. Overall, this review highlights current knowledge gaps and summarizes potential strategies for support of efforts to improve heme production in microalgae and cyanobacteria.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104823"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Galactomannan-based sustainable functional biopolymeric material for efficient harvesting of Chlorella sorokiniana","authors":"Niwas Kumar,&nbsp;Namrata Bhagat,&nbsp;Subham Sekhar Mandal,&nbsp;Pralay Maiti,&nbsp;Pratyoosh Shukla","doi":"10.1016/j.algal.2026.104806","DOIUrl":"10.1016/j.algal.2026.104806","url":null,"abstract":"<div><div>Microalgae serve as sustainable biofactories for bioenergy and bioproducts, yet their biomass harvesting remains a critical bottleneck because of low culture density and the inherent instability caused by negative zeta potential. Conventional flocculants often contaminate biomass with toxic residues or metallic ions, resulting in difficulties in efficient downstream processing of microalgal biomass. Thus, it is essential to develop natural, non-toxic, and biocompatible materials for efficient microalgal dewatering. A cationic locust bean gum (GLBG) polymer was synthesized via graft copolymerization of [2-(Methacryloyloxy)ethyl] trimethylammonium chloride (METAC) onto locust bean gum. The polymer was characterized using FTIR, NMR, elemental analysis, XRD, and zeta potential measurements. Its harvesting efficiency was evaluated against <em>Chlorella sorokiniana</em>. Also, cytocompatibility was assessed through trypan blue staining, chlorophyll fluorescence, and in vitro fibroblast cell viability assays. The effect of GLBG on metabolite extraction was examined for lipids, proteins, and carbohydrates. GLBG achieved &gt;97% harvesting efficiency at an optimum dosage of 25 ppm. It did not interfere with lipid, protein, or carbohydrate recovery from biomass and preserved microalgal cell integrity and photosynthetic performance. Cytocompatibility assays confirmed non-toxicity, positioning GLBG as a sustainable, biodegradable, and efficient biopolymeric material for microalgal biomass recovery and downstream applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104806"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of carbon-coated zero-valent iron nanoparticle suspensions supplementation on the performance of high-rate algal pond treating biogas and wastewater","authors":"Rentaro Kuri,&nbsp;Bruna Sampaio de Mello,&nbsp;Edwin G. Hoyos,&nbsp;Saúl Blanco,&nbsp;Tatsuki Toda,&nbsp;Raúl Muñoz","doi":"10.1016/j.algal.2026.104822","DOIUrl":"10.1016/j.algal.2026.104822","url":null,"abstract":"<div><div>This study assessed the effect of carbon-coated zero-valent iron nanoparticle (ZVI-NPs) suspension on enhancing photosynthetic biogas upgrading. A continuous photobioreactor system co-cultivating microalgae and bacteria was operated using synthetic biogas and real digestate to evaluate microalgal growth, photosynthetic performance, and CO₂ removal with ZVI-NPs suspension. The addition of ZVI-NPs suspension notably increased biomass concentration (up to 3.1 g L⁻¹) and photosynthetic efficiency (up to 0.64). These enhancements raised the pH of the cultivation broth to 9.3 and improved CO₂ mass transfer, resulting in a CO₂ removal efficiency of 95.0% and an average CH₄ content of 91.9% in the upgraded biogas, meeting grid-injection standards. Although bicarbonate supplementation was required to avoid inorganic carbon limitation, the ZVI-NPs significantly reduced the overall inorganic carbon demand by boosting photosynthesis. Overall, these findings demonstrate that ZVI-NPs suspensions can effectively enhance microalgal performance and CO₂ capture, supporting their application as sustainable and cost-effective strategy for biogas upgrading.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104822"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148567180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated pond co-culture of Penaeus vannamei with red seaweeds: Implications for nutrient uptake and dual production performance","authors":"K. Surya,&nbsp;Madhuri Pathak,&nbsp;Munilkumar Sukham,&nbsp;Thongam Ibemcha Chanu,&nbsp;Layana Porayil,&nbsp;Vaibhav A. Mantri,&nbsp;Debajit Sarma,&nbsp;G. Harini,&nbsp;P.S. Prathib,&nbsp;Bhavik Kantilal Bhagiya,&nbsp;Komal R. Chilveri","doi":"10.1016/j.algal.2026.104794","DOIUrl":"10.1016/j.algal.2026.104794","url":null,"abstract":"<div><div>The integration of seaweeds into shrimp aquaculture offers a sustainable strategy for nutrient bioremediation and productivity enhancement. Despite its potential, comparative nutrient dynamics, shrimp performance, and seaweed bioactive responses in semi-intensive pond co-culture systems along the Maharashtra coast remain insufficiently explored. A 56-day pond experiment was conducted in a completely randomized design with three treatments: shrimp monoculture (C), shrimp co-cultured with <em>K. alvarezii</em> (T1), and with <em>G. foliifera</em> (T2), with seaweed monolines stocked at 1 g L⁻¹ introduced on day 45 of shrimp culture. Compared to the control, co-culture treatments, especially with <em>Gracilaria foliifera</em> (T2) significantly improved water quality by reducing nitrate, nitrite, total ammonia nitrogen, and phosphate by 90.1%, 72.1%, 57%, and 75.1%, respectively. Co-cultured shrimp, particularly in T2, showed enhanced growth performance, with higher final weight (19.13 g), weight gain (812.74%), specific growth rate (3.94% day⁻¹), and yield (0.12 kg m⁻³) compared to monoculture. Physiological assessments revealed reduced oxidative stress, improved immune status, healthier tissue histology, and increased protein content in co-cultured shrimp. While <em>K. alvarezii</em> achieved higher biomass increment, <em>G. foliifera</em> contributed more effectively to nutrient removal and shrimp performance. Co-cultured seaweeds also exhibited elevated protein, lipid content, enhanced elemental profile, with co-cultured seaweeds also exhibiting improved protein, lipid content, and elemental composition compared to pre-co-culture biomass. Bioactive properties were influenced by co-culture conditions and solvent polarity, with aqueous solvents enhancing phenolic content and organic solvents improving antioxidant activity. Shrimp–seaweed co-culture, particularly with <em>G. foliifera</em>, offers farmers a practical and scalable approach to improve water quality, enhance shrimp yield, and generate additional income from value-added seaweed biomass.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104794"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and transcriptomic responses of Synechococcus sp. FACHB-805 to phosphite under phosphate limitation","authors":"Ye Zheng,&nbsp;Xingyao Ye,&nbsp;Xiaojun Niu,&nbsp;Dongqing Zhang,&nbsp;Mo Wang,&nbsp;Yang Yu,&nbsp;Ling Li,&nbsp;Chen Wang,&nbsp;Xiufang Yang,&nbsp;Xintai Su","doi":"10.1016/j.algal.2026.104801","DOIUrl":"10.1016/j.algal.2026.104801","url":null,"abstract":"<div><div>With the increasing detection of phosphite (Phi) in aquatic systems, growing concern has emerged regarding its bioavailability and potential consequences for microbial nutrient utilization and carbon cycling. However, whether Phi can functionally substitute for phosphate (Pi), and how Phi exposure modifies cellular physiology and stress responses under Pi-deficient conditions, remain insufficiently understood in freshwater cyanobacteria. Here we systematically investigated the physiological, biochemical, ultrastructural, and transcriptional responses of the freshwater cyanobacterium <em>Synechococcus</em> sp. FACHB-805 to a Phi exposure gradient under contrasting Pi regimes. The results showed that <em>Synechococcus</em> sp. FACHB-805 lacks the functional <em>ptxD</em>-mediated enzymatic machinery required to oxidize phosphite into metabolically usable orthophosphate. This genetic deficiency fundamentally underpins the strain's inability to sustain population growth, chlorophyll-a accumulation, and Rubisco activity under phosphite-sole conditions. Elevated Phi exposure under Pi-limitation did not relieve the Pi-starvation state and was associated with higher malondialdehyde content and superoxide dismutase activity, together with transcriptional signatures suggestive of redox-stress-related adjustment and genome-maintenance-related responses. Together, these results indicate that Phi, even when unable to support growth as a sole phosphorus source, can shape the oxidative state, carbon fixation efficiency, and dissolved organic carbon release of dominant freshwater cyanobacteria under Pi limitation, underscoring the importance of incorporating phosphorus redox chemistry into assessments of microbial carbon cycling in P-limited aquatic ecosystems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104801"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-energy biorefinery of Tetradesmus obliquus using hydrodynamic cavitation for protein extraction and bioenergy recovery","authors":"Dilson Novais Rocha,&nbsp;Natália dos Santos Renato,&nbsp;Alisson Carraro Borges,&nbsp;André Pereira Rosa,&nbsp;Maurício de Oliveira Leite,&nbsp;Marcio Arêdes Martins","doi":"10.1016/j.algal.2026.104804","DOIUrl":"10.1016/j.algal.2026.104804","url":null,"abstract":"<div><div>The development of microalgal biorefineries depends on energy efficiency and costs associated with wet biomass processing. In this work, alternative processing routes for <em>Tetradesmus obliquus</em> BR003 were evaluated by combining hydrodynamic cavitation (HC) for cell disruption with protein and lipid extraction, followed by anaerobic digestion of the residual biomass. Using HC, the yields of protein extraction (PE) and lipid extraction (LE) were 36% and 46.5%, respectively. When extracting lipids from deproteinized biomass (PLE), the yield was lower (32.1%) indicating that part of the lipids was co-extracted during the protein recovery step. Anaerobic digestion allowed increased energy recovered by 67.5% and 68.7% from LE and PLE biomass, respectively, with the use of HC. The HC showed a 5.5 times lower energy consumption than ultrasound disruption (US), and the lipid extraction route followed by anaerobic digestion of residual biomass yielded the highest net energy ratio (NER). Hydrodynamic cavitation is an effective method for cell disruption with low energy consumption. Although routes including protein extraction showed lower net energy ratios, the extraction of this compound is a promising strategy to add value to the microalgal production chain, since the price of this product is eight times higher than the market price of microalgae oil. The novelty of this study consists on the experimental integration of hydrodynamic cavitation followed by extraction processes and anaerobic digestion, combined with a consistent energy balance to identify energetically favorable processing routes.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104804"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetically driven carbonate precipitation by Chlorella vulgaris controls copper immobilization under non-equilibrium conditions","authors":"José San Martín,&nbsp;Alexander Kusianovich,&nbsp;Robinson Soto-Ramírez,&nbsp;Mariella Rivas,&nbsp;David Jeison,&nbsp;Álvaro Torres-Aravena","doi":"10.1016/j.algal.2026.104800","DOIUrl":"10.1016/j.algal.2026.104800","url":null,"abstract":"<div><div>Photosynthetically driven microbially induced carbonate precipitation (MICP) has emerged as a promising pathway for metal immobilization; however, the mechanisms governing mineral formation under microalgal conditions remain poorly understood. In this study, copper immobilization was investigated using <em>Chlorella vulgaris</em> across a wide concentration range (0.008–50 mM Cu), combining batch experiments, adsorption controls, geochemical modeling, and FTIR characterization. Photosynthetic activity controlled pH dynamics through CO₂ uptake, promoting carbonate supersaturation and mineral formation. Copper removal reached up to 90% at 0.05 mM, while abiotic precipitation dominated at both low (0.008 mM) and high concentrations (≥1 mM), indicating a shift from biologically influenced to chemically controlled regimes. Adsorption contributed less than 10%, suggesting a secondary role primarily associated with heterogeneous nucleation. Equilibrium modeling predicted well-defined carbonate phases such as malachite, whereas FTIR analysis identified spectral features consistent with poorly crystalline, organic-associated carbonate phases and Cu<img>O bonding environments, supporting non-equilibrium mineralization pathways. These results demonstrate that mineral formation in photosynthetic MICP systems is governed by the coupling between microalgal activity and solution chemistry, rather than by equilibrium conditions alone. The findings highlight the importance of biological interfaces and transient physicochemical conditions in controlling mineralization pathways, with direct implications for the design and optimization of microalgae-based systems for metal removal.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104800"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient management strategy with optimum application regime in the globally farmed red seaweed Kappaphycus alvarezii enhances biomass production without compromising hydrocolloid yield and quality","authors":"Bienson Ceasar V. Narvarte,&nbsp;Tom Gerald T. Genovia,&nbsp;Michael Y. Roleda","doi":"10.1016/j.algal.2026.104812","DOIUrl":"10.1016/j.algal.2026.104812","url":null,"abstract":"<div><div>As demand for sustainable food ingredients rises, seaweed aquaculture has become increasingly important sector for food security, coastal livelihoods, and reducing pressure on terrestrial food systems. Carrageenan-producing eucheumatoids, particularly <em>Kappaphycus alvarezii</em>, underpin global supplies of food-grade hydrocolloids used in foods and nutraceuticals. However, the productivity and reliability of eucheumatoid farming systems, largely operated by small-scale farmers in tropical developing countries, are increasingly constrained by nutrient limitation, climate-driven environmental stress, and rising production costs. In response, farmers often apply inorganic fertilizers indiscriminately to boost biomass production, since trading depends only on raw dried seaweed (RDS) biomass, without considering the extractable hydrocolloid yield and quality. In this study, we assessed growth, morphology, and biochemistry of <em>K. alvarezii</em> under control, single-pulse (1×), and intermittent (5×) ammonium phosphate fertilization over 50 days. Intermittent supplementation enhanced growth rates (2.19–4.34% d⁻¹), branching density (6.06 ± 0.23 branchlet cm⁻¹), chlorophyll <em>a</em> and <em>d</em> (1.88–6.81 and 5.70–7.30 mg g⁻¹ FW, respectively), and protein content (1.65–2.94 %FW) of <em>K. alvarezii</em>, reflecting sustained nitrogen assimilation. However, carrageenan yield was comparable between control (41.16 ± 6.77 %DW) and 1× (39.70 ± 1.60 %DW) treatments but declined under intermittent fertilization (36.90 ± 2.10 %DW), despite no detectable effects on gel strength and viscosity. Our study provided evidence that intensified nutrient supplementation uncouples biomass production from carrageenan yield in farmed <em>K. alvarezii</em>. A “<em>boost-and-rest</em>” strategy, i.e., short-term or one-time nutrient enrichment followed by nutrient (N) limitation, stimulates early growth while maintaining carrageenan yield, reducing input costs, and enhancing sustainability. Optimizing nutrient application regime, when necessary and sanctioned, can improve farm efficiency, strengthen coastal livelihoods, and contribute to resilient seaweed-based food systems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104812"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Freshwater microalgae strain selection for industrial triacylglycerol production – a scale-down approach","authors":"Juliëtte S. Camstra ,&nbsp;Pepijn van Mierlo ,&nbsp;Inês Trincheiras ,&nbsp;Rick Wieggers ,&nbsp;Maria J. Barbosa ,&nbsp;Marcel Janssen","doi":"10.1016/j.algal.2026.104788","DOIUrl":"10.1016/j.algal.2026.104788","url":null,"abstract":"<div><div>Microalgae are a promising source of sustainable lipids, particularly triacylglycerols (TAGs), but large-scale production remains economically unviable and depends on strain selection. In this study, four microalgal strains were selected based on their ability to accumulate TAG: <em>Tetradesmus obliquus wild type</em> (<em>wt)</em>, <em>Tetradesmus obliquus</em> starchless mutant 1 (slm1), <em>Chromochloris zofingiensis</em>, and <em>Neochloris oleoabundans</em>. These strains were compared on growth rate, areal fatty acid productivity, and photosynthetic parameters under diurnal light and temperature cycles and nitrogen-sufficient and nitrogen-deficient conditions. This scale-down approach integrates laboratory screening under environmental fluctuations, providing novel insight into strain performance relevant for large-scale TAG production. Under nitrogen sufficiency, <em>T. obliquus wt</em> and <em>slm1</em> showed the highest growth, reaching 10.0 ± 0.2 (n = 2) and 9.7 ± 0.2 (n = 2) g m⁻² day⁻¹. Under nitrogen starvation, only slm1 maintained growth at 8.4 ± 1.6 g m⁻² day⁻¹, as productivity in other strains declined significantly. Additionally, <em>T. obliquus slm1</em> reached the highest total fatty acid content with 44.7 ± 3.7%, with 38.8 ± 2.8% of TAG. Therefore, <em>T. obliquus slm1</em> emerged as the highest TAG producer and reached an average areal productivity of 7.3 ± 1.1 g_TAG m⁻² day⁻¹ over the second week of starvation. The potential of this strain was demonstrated in a 275 L vertically stacked tubular photobioreactor, achieving an average volumetric biomass productivity of 0.53 ± 0.06 g L⁻¹ day⁻¹ during the growth and 0.28 ± 0.06 g L⁻¹ day⁻¹ during the stress phase. These values correspond to areal biomass productivities of 25.2 ± 2.8 g m⁻² day⁻¹ and 13.48 ± 3.01 g m⁻² day⁻¹. After 21 days of stress, the lipid content reached 42.9 ± 0.9% TFA and 32.5 ± 0.5% TAG.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104788"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148342799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}