Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Role of algae in modulating human lipid profiles from metabolic pathways to biotechnological applications","authors":"Vishal Kumar ,&nbsp;Deepika Kaushik ,&nbsp;Harmandeep Kaur ,&nbsp;Emel Oz ,&nbsp;Charles Brennan ,&nbsp;Charalampos Proestos ,&nbsp;Fatih Oz ,&nbsp;Mukul Kumar","doi":"10.1016/j.algal.2025.104149","DOIUrl":"10.1016/j.algal.2025.104149","url":null,"abstract":"<div><div>Lipids are vital organic compounds that serve crucial functions in energy storage, cellular architecture, and signaling processes. Algal lipids rich in polyunsaturated fatty acids, particularly omega 3 and omega 6, have garnered attention for their purported advantages, including the resolution of inflammation, cardiovascular protection, and regulation of metabolism. This detailed investigation delves into the importance of lipids, especially those derived from algae. Their potential offers a valuable pathway for acquiring a wide range of crucial fatty acids and bioactive substances that may enhance human well-being. The manuscript explores the biosynthesis of algal lipids, detailing the related metabolic pathways and their potential integration to improve lipid profiles in humans. Moreover, the study highlights the mechanisms of lipid transport and metabolism in human systems, the relationship between algal lipids and gut microbiota, and the recent innovations in biotechnology focused on improving algal lipid production. This study further investigates their effectiveness in tackling neuroinflammation and metabolic disorders. In conclusion, the utilization of algae lipids and their incorporation into functional foods and nutraceuticals offers a promising alternative to current techniques for sourcing PUFAs, underscoring their significance in modern dietary habits.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104149"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of Baltic macroalgae for food preservation","authors":"Provalenta A. Tirtantyo ,&nbsp;Martina Blümel ,&nbsp;Claudia Welsch ,&nbsp;Arlette Wenzel-Storjohann ,&nbsp;Deniz Tasdemir","doi":"10.1016/j.algal.2025.104141","DOIUrl":"10.1016/j.algal.2025.104141","url":null,"abstract":"<div><div>Despite all technological developments, food spoilage still remains a major economic, environmental and health concern. This study aimed to assess the potential of eight Baltic macroalgae for food preservation, specifically for antimicrobial and antioxidant active food packaging applications. Three brown, three red and two green macroalgae species were extracted automatically by Accelerated Solvent Extraction (ASE) and Supercritical Fluid Extraction (SFE) and evaluated for their antimicrobial effect against nine foodborne pathogens, antioxidant capacity and total phenolic content (TPC). The ASE and SFE extracts of brown algae (<em>Fucus</em> spp.) showed the highest antimicrobial and antioxidative activities and the greatest TPCs<em>. Fucus distichus</em> subsp. <em>evanescens</em> exhibited the highest inhibition against <em>Staphylococcus aureus</em> with IC₅₀ values of 2.1 μg/mL (ASE, H₂O:ethanol (50:50) extract) and 4.4 μg/mL (SFE, CO₂:Ethanol (50:50). The ethanolic (100 %) ASE and CO₂:Ethanol (20:80) SFE extracts of <em>Fucus serratus</em> showed the best antioxidant capacity (IC₅₀s 37.9 μg/mL (ASE) and 24.6 μg/mL (SFE)) and the highest TPCs (158 mg GAE/g (ASE) and 297.41 mg GAE/g (SFE)). Bioactive extracts were further analyzed by UPLC-MS/MS-based untargeted metabolomics. The main components of all extracts were polar lipids (galactolipids and betaine lipids), along with carotenoids, phlorotannins and chlorophylls. These results provide insights into the bioactivity and chemical diversity of Baltic macroalgae, highlighting the potential of <em>Fucus</em> spp. for food preservation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104141"},"PeriodicalIF":4.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated microalgae biorefinery using produced water: Simultaneous obtaining of biomass, biofuels and exopolysaccharides","authors":"Bianca Bomfim Andrade ,&nbsp;Carolina Oliveira de Souza ,&nbsp;Natália Hlavnicka Miranda ,&nbsp;Jadson dos Santos França ,&nbsp;Ana Teresa Lombardi ,&nbsp;Silvana Mattedi e Silva ,&nbsp;Denilson de Jesus Assis ,&nbsp;Jania Betania Alves da Silva ,&nbsp;Fabio Alexandre Chinalia ,&nbsp;Lucas Guimarães Cardoso","doi":"10.1016/j.algal.2025.104140","DOIUrl":"10.1016/j.algal.2025.104140","url":null,"abstract":"<div><div>Produced water (PW), a waste product of the oil industry, can be integrated into microalgae biorefinery systems not only for its bioremediation but also for the production of industrially relevant biomolecules, supporting the circular economy. Accordingly, the growth of microalgal species <em>Chlorolobion braunii</em>, <em>Chlorella zofingiensis</em>, <em>Monoraphidium griffithii</em> (freshwater), <em>Dunaliella tertiolecta</em>, <em>Isochrysis galbana</em>, and <em>Phaeodactylum tricornutum</em> (marine) was tested in media containing PW concentrations from 0 % to 100 %. PW concentrations &gt;50 % supported microalgae growth, with the highest biomass production observed for <em>I. galbana</em> at PW80% (1.58 g L⁻¹). Among the tested species, <em>D. tertiolecta</em> exhibited the highest lipid content at PW80% (37.26 %), while <em>C. braunii</em> showed the highest carbohydrate content at PW50% (24.51 %). The biodiesel produced in PW exhibited a fatty acid profile comparable to that obtained in the standard medium, with characteristics similar to those of commercially available biodiesel, highlighting the potential of PW as a substrate for biorefinery processes. The tested microalgae also produced bioethanol in quantities exceeding those reported in the literature for cultures grown in the standard medium (0.60–16.81 mL). Cultivation in PW also led to substantial production of exopolysaccharides, particularly by <em>I. galbana</em>, which yielded 0.56 g L⁻¹ at PW80%. This species also demonstrated high bioremediation efficiency, achieving complete removal of phosphorus and nitrate and reducing iron concentrations by &gt;88 % in the PW80% medium. These results demonstrate the technical feasibility of using PW for microalgae cultivation and the concurrent production of biofuels and EPS while also supporting effective waste bioremediation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104140"},"PeriodicalIF":4.6,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination of Sargassum wightii extract and pyridoxin mixture improves growth and salinity tolerance in Okra for sustainable agriculture","authors":"Zawar Khan ,&nbsp;Humaira Gul ,&nbsp;Aliya Rehman ,&nbsp;Asma Tabassum ,&nbsp;Mamoona Rauf ,&nbsp;Muhammad Arif ,&nbsp;Muhammad Hamayun ,&nbsp;Mohamed A. El-Sheikh ,&nbsp;Zainul Abideen","doi":"10.1016/j.algal.2025.104145","DOIUrl":"10.1016/j.algal.2025.104145","url":null,"abstract":"<div><div>Salinity is a major problem for food security and sustainable development in the world. Applying effective seaweeds and pyridoxine mixture offer a potential in the form of bio-stimulants for improving plant growth and ecophysiological output. This study was designed to compare impact of <em>Sargassum wightii</em> extract (2 and 4 %) and pyridoxine 0.04 % and their mixture for the first time on <em>Abelmoschus esculentus</em> plants under 125 mM NaCl to enhance its salt tolerance and biomass production. Plant growing with the <em>S. wightii</em> extract (2 and 4 %) and pyridoxine 0.04 % mixture stimulated the growth parameters around (28 to 107 %) compared to their sole effects. Spraying of <em>S. wightii</em> aqueous extract (2 and 4 %) and pyridoxine (0.04 %) alone and in combination improved the relative water contents (57 % in control, 16–78 % in salinity) and essential minerals uptake (26–40 %) while reduced (40 %) sodium levels for optimum ion homeostasis especially in salinity. Plants treated with <em>S. wightii</em> extract and pyridoxin mixture enhanced antioxidant enzyme activities but hydrogen peroxide, catalase and abscisic acid, were reduced. Application of <em>S. wightii</em> (4 %) with pyridoxine (0.04 %) worked as natural bio-stimulants for amelioration of salt stress and biomass production of okra.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104145"},"PeriodicalIF":4.6,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The metabolome of crustose coralline algae is driven by phylogeny and environmental conditions","authors":"Camille Vizon ,&nbsp;Laura Lagourgue ,&nbsp;Hendrikje Jorissen ,&nbsp;Delphine Raviglione ,&nbsp;Claude E. Payri ,&nbsp;Isabelle Bonnard ,&nbsp;Maggy M. Nugues","doi":"10.1016/j.algal.2025.104146","DOIUrl":"10.1016/j.algal.2025.104146","url":null,"abstract":"<div><div>Metabolomics is a powerful approach to investigate the effect of environmental conditions on metabolite variations in marine algae. Here, we focused on crustose coralline algae (CCA), a group of calcifying, red algae which play an important role on coral reefs through their interactions with corals and contribution to coral reef formation. Despite their ecological importance, little is known about their metabolome and how it varies with environmental conditions and phylogeny. Using an untargeted metabolomic approach, we explored the metabolomic fingerprints of seven CCA species (order: Corallinales) commonly found on the coral reefs of Moorea, French Polynesia. We developed an extraction method to characterize the CCA metabolome on two sample types (whole fragment and surface only) and explored the chemical variations of CCA across species, reef habitat and microhabitat. The extraction method successfully characterized the CCA metabolome, as demonstrated by a technical variability lower than the biological variability for both sample types. The CCA metabolome was species-specific and a correlation was found between phylogenetic taxonomy and metabolomic profiles of the different species. Moreover, the metabolomic composition of certain species differed between the back and fore reef habitats and between exposed and cryptic microhabitats. These results highlight a high variability in the CCA metabolome mediated by phylogeny and environmental conditions. This study provides valuable insights into the sources of metabolomic variation in CCA. It lays the groundwork for exploring the ecological functions of the CCA metabolome and its potential use as a tool to assess organismal and ecosystem health.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104146"},"PeriodicalIF":4.6,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Edible fungi pellets as an effective carrier of microalgae biomass and fish waste hydrolysates","authors":"María Laura Miño,&nbsp;Pedro Darío Zapata,&nbsp;María de los Angeles Kolman","doi":"10.1016/j.algal.2025.104139","DOIUrl":"10.1016/j.algal.2025.104139","url":null,"abstract":"<div><div>Microalgae are a promising resource for food and feed, but their large-scale application is limited by the high cost of biomass harvesting. Fungal-assisted bioflocculation offers a sustainable alternative; however most reported fungal strains are inedible, limiting their use in food and feed applications.</div><div>This study evaluates the efficiency of the edible fungi <em>Pleurotus pulmonarius</em> LBM105 and <em>Lentinus sajor-caju</em> LBM266 to flocculate four native microalgae strains from Misiones, Argentina. The effect of a sugarcane bagasse extract (SBE) based medium on flocculation efficiency (FE) and biomass composition was assessed.</div><div>Results showed that <em>P. pulmonarius</em> LBM105 pellets obtained in malt extract (ME) medium achieved a FE of 96 % after 24 h when co-cultivated with <em>G. emersonii</em> CMI015. In contrast, <em>L. sajor-caju</em> LBM266 pellets exhibited a lower FE (65–71 %) in the standard medium but reached 100 % in SBE medium when co-cultivated with CMI015 and CMI016. ATR-FTIR analysis suggested the involvement of amino, amide, phosphate, hydroxyl, and carboxyl groups in fungal–microalgal interactions. Biomass characterization of the fungal-microalgae pellets (FMP) revealed compositional shifts with increased lipid and protein content in <em>P. pulmonarius</em> LBM105 + CMI015 pellets. FMPs were further enriched with fish waste hydrolysate (FWH). In lyophilized <em>P. pulmonarius</em> + CMI015 pellets, FWH supplementation increased both lipid, protein and total phenolic content (TPC) levels, while in L. <em>sajor-caju</em> + CMI016 only TPC increases. This strategy of FWH enrichment using lyophilized FMPs as carriers represents a sustainable method for incorporating bioactive compounds into biomass. These findings highlight the potential of FWH-enriched fungal–microalgae pellets for use in food and feed applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104139"},"PeriodicalIF":4.6,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PERCEPTIVE: an R shiny pipeline for the prediction of epigenetic modulators in novel species","authors":"Eric M. Small,&nbsp;Christina R. Steadman","doi":"10.1016/j.algal.2025.104142","DOIUrl":"10.1016/j.algal.2025.104142","url":null,"abstract":"<div><div>Epigenetic processes are central to regulating gene expression, genome stability, and metabolic function across the tree of life; yet, their roles remain underexplored in microalgae, especially as new species continue to be identified and characterized. This is likely due to the cumbersome nature and species-dependent attributes of epigenetic wet-lab methodologies, which preclude the rapid identification of epigenetic modifications and modulators. However, there is high conservation of epigenetic processes from budding yeast to humans; in many cases, one may infer how behavior and function are epigenetically regulated in novel species by identifying epigenetic modulators, or the proteins responsible for conferring epigenetic modifications. To this end, we have developed a graphical software package, titled PERCEPTIVE (<u>p</u>ipelin<u>e</u> for the p<u>r</u>edi<u>c</u>tion of <u>ep</u>igenetic modula<u>t</u>ors <u>i</u>n no<u>v</u>el sp<u>e</u>cies). This platform solely uses the genomic sequence of an algal species, and preexisting information from other model organisms, to predict the epigenetic modulators and associated modifications in algae. Predictions are presented to the user in a graphical interface, which provides literature-based interpretation of results, enabling users to quickly understand potential epigenetic processes in their algal species of interest and plan follow-up experiments. To test PERCEPTIVE, we predicted epigenetic modulators in several feedstock candidate algae species. To validate these predictions, wet-lab studies were performed, including mass spectrometry; these results underscore the high accuracy of PERCEPTIVE predictions. Overall, PERCEPTIVE represents a powerful in silico tool for the research and manipulation of algal species, which does not require a priori knowledge of epigenetics and is accessible to a broad set of investigators.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104142"},"PeriodicalIF":4.6,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How sticky is sticky? A note on gametophyte adherence of the macroalga Saccharina latissima (Phaeophyceae)","authors":"Eoghan Mánus Cunningham ,&nbsp;Pamela J. Walsh ,&nbsp;Louise Kregting","doi":"10.1016/j.algal.2025.104138","DOIUrl":"10.1016/j.algal.2025.104138","url":null,"abstract":"<div><div>Temperate marine systems are ideal environments for expanding global seaweed aquaculture, yet high costs, particularly from macroalgal nursery facilities, have become a limiting factor for industry development. Recent research has explored the use of binder-seeding, whereby juvenile kelp life stages are seeded directly onto rope using adhesive solutions and deployed directly at sea. This method is used to reduce our reliance on nursery facilities and reduce aquaculture costs. While binder-seeding has shown mixed results, a more simplistic approach by Klaus Lüning has received less attention. Klaus Lüning (1979) commented that kelp gametophytes were naturally ‘sticky’ and attach to substrata within ‘a short time’, potentially highlighting a natural and more cost-effective seeding technique. Here, we explored the natural adherence of <em>Saccharina latissima</em> gametophytes to aquaculture twine. Cultures were sprayed onto twine and left to adhere for eight increasing time periods, before being submerged in seawater and measured after 10 weeks of growth. Gametophytes were found to adhere and grow successfully on twine after immediate submergence in seawater, however significant differences in sporophyte lengths and density after 10 weeks growth were observed among time treatments. Sporophyte lengths and densities were found to be highest when the culture was left to adhere for 30 min prior to submergence in seawater, potentially highlighting a time-sensitive sweet spot for natural gametophyte seeding. Further, we confirm that Lüning's observation of kelp gametophytes sticking to substrata after a ‘short time’ was in fact ‘immediate’, thereby shorter than previously thought.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104138"},"PeriodicalIF":4.6,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contradictive effects of light on diarrhetic shellfish toxin production-depending on photosynthetic status and photoprotection ability of Prorocentrum lima strains","authors":"Heng Chen ,&nbsp;Ruiwang Wei ,&nbsp;Zehui Huang ,&nbsp;Hua Zhang ,&nbsp;Kaixuan Huang ,&nbsp;Songhui Lu","doi":"10.1016/j.algal.2025.104135","DOIUrl":"10.1016/j.algal.2025.104135","url":null,"abstract":"<div><h3>Introduction</h3><div>Diarrhetic shellfish toxins (DST), including okadaic acid (OA) and its analogues, are potent inhibitors of protein phosphatases and can be transported via food chain, potentially leading to human poisoning. Their biosynthesis in <em>Prorocentrum</em> and <em>Dinophysis</em> genera is light-dependent; however, it can be inhibited by high or ultraviolet radiation (UV) in our recent study.</div></div><div><h3>Methods</h3><div>Based on the hypothesis that photosynthetic status is relevant to DST production, two <em>Prorocentrum lima</em> strains (SD4, light-tolerant; DS4G4, light-sensitive) were exposed to different light conditions (12–400 μmol photons m⁻² s⁻¹, with/without UV-A) for 30 days.</div></div><div><h3>Results</h3><div>Initially, photosynthetic activity (<em>F</em>_<em>v</em><em>/fm</em>) was inhibited by high light/UV-A, then recovered. Notably, the DST production rate (<em>R</em>_tox) decreased exponentially with increasing light on day 6, more significantly in strain DS4G4. During photoacclimation (day 6 – day 18), <em>R</em>_tox increased and attained a maximum (<em>R</em>_max), which in all cases was exponentially increased with light intensity. Under PAR conditions, the half-light saturation intensity (<em>I</em>_k) for <em>R</em>_max of OA did not surpass 25 μmol photons m⁻² s⁻¹ in both strains, highlighting the relatively low light requirements for DST production. Additionally, on day 6, chlorophyll <em>a</em> production rate (<em>R</em>_{Chl a}) demonstrated different correlations with <em>R</em>_tox under low and high light.</div></div><div><h3>Conclusion</h3><div>DST production is tightly associated with photosynthetic status and strain-specific photoprotection ability. The inhibition of DST by light is an inevitable phenomenon, and the underlying relationship between toxin production and photoinhibition process was further explored and discussed.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104135"},"PeriodicalIF":4.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the potency of rice field cyanobacterial strains for plant growth promotion and root colonization by deciphering the molecular elicitors","authors":"Manickam Gayathri,&nbsp;Sumathy Shunmugam,&nbsp;Gangatharan Muralitharan","doi":"10.1016/j.algal.2025.104131","DOIUrl":"10.1016/j.algal.2025.104131","url":null,"abstract":"<div><div>Soil cyanobacteria collected from paddy fields across six diverse locations were evaluated for their plant growth-promoting potential, specifically through cyanobacterial extracellular products (CEP) influencing <em>Pisum sativum</em> L. germination and subsequent morphometric traits. Out of 20 tested strains, <em>Anabaena</em> sp. MBDU 902 demonstrated radicle and plumule lengths of 4.59 cm and 1.75 cm, respectively, while <em>Nostoc microscopicum</em> MBDU 102 exhibited enhanced radicle and plumule growth responses of 60 % and 80 %. Strain prioritization was achieved through Preference Ranking Organization Method for Enrichment Evaluation (PROMETHEE) and Graphical Analysis for Interactive Assistance (GAIA), where <em>Nostoc commune</em> MBDU 101 emerged as the most potent strain based on aggregate growth metrics. The bioactive extract of <em>Nostoc commune</em> MBDU 101 was purified and analyzed using Preparative High-Performance Liquid Chromatography (P-HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS), identifying key phytohormones, including Indole-3-butyric acid (IBA), Gibberellic acid, IAA conjugates, and long chain fatty acids, confirmed via agar slant bioassay. To elucidate its root association capabilities, hydroponic cultivation of <em>Zea mays</em> L. was conducted using <em>Nostoc commune</em> MBDU 101 culture and CEP, resulting in significant enhancement of seedling length, biomass, leaf dimensions, and lateral root development compared to control. Root colonization was validated through confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Gas Chromatography-Mass Spectrometry (GC–MS) revealed signaling molecules in culture filtrates essential for root association, underscoring <em>Nostoc commune</em> MBDU 101 robust plant growth-promoting attributes.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104131"},"PeriodicalIF":4.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}