Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"In vitro evaluation of functional properties of extracts of Fucus vesiculosus obtained with different conventional solvents","authors":"Luciana Rossi ,&nbsp;Benedetta Canala ,&nbsp;Anna Paola Fifi ,&nbsp;Sara Frazzini","doi":"10.1016/j.algal.2024.103787","DOIUrl":"10.1016/j.algal.2024.103787","url":null,"abstract":"<div><div><em>Fucus vesiculosus</em> is a rich source of bioactive substances with many biochemical functions that provide it a variety of biological effects. Over the years, significant research efforts have been made to extract bioactive compounds by applying different methodologies for various applications. There are several solvents used for the extraction of natural products since the choice of solvent must be based primarily on the characteristics of the matrices and the properties of the molecular classes to be obtained. Therefore, the aim of this study was to investigate the efficiency of different conventional solvents to maximize the yield of polyphenol and flavonoid content as well as the antioxidant, antimicrobial and anti-inflammatory capacity. The different extracts of <em>F. vesiculosus</em> were analyzed for the Total Polyphenol Content (TPC) and the Total Flavonoid Content (TFC). As well the antioxidant, antimicrobial, and anti-inflammatory capacities were evaluated. The results concerning the content of bioactive molecules disclosed that the extraction carried out with the methanol (50 %) was the one that gave the highest yield in both polyphenol (2.27 ± 0.17 mg GAE/ 50 mg of sample) and flavonoid content (187.12 ± 12.86 mg CE/50 mg of sample) compared to acetone and ethanol extracts. Regarding the functional properties, the results obtained disclose that the extract of <em>F. vesiculosus</em> had a high antioxidant capacity (90 % inhibition of radical scavenging activity). Additionally, the growth inhibition assay disclosed that <em>F. vesiculosus</em> can reduce significantly (<em>p</em> &lt; 0.05) the growth of <em>E. coli</em> F18⁺, in particular when the alga is extracted with methanol and acetone. As well, a concentration of 1 mg/mL of <em>F. vesiculosus</em> inhibits the protein denaturation by 60 %, highlighting a potential anti-inflammatory activity. In conclusion, this study discloses the richness of bioactive molecules in <em>F. vesiculosus</em> and the resulting functional properties, highlighting also the power of methanol as extraction solvent.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103787"},"PeriodicalIF":4.6,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative technology for microalgal cell preservation through immobilization in polylactic acid nanofibers","authors":"Ana Claudia Araujo de Almeida ,&nbsp;Bruna da Silva Vaz ,&nbsp;Jorge Alberto Vieira Costa ,&nbsp;Michele Greque de Morais","doi":"10.1016/j.algal.2024.103781","DOIUrl":"10.1016/j.algal.2024.103781","url":null,"abstract":"<div><div>Microalgae are of great biotechnological importance. Thus, it is essential to apply maintenance methods for the utilization of microalgae at any time. Facilitating microalgae adsorption processes on nanofibers may be a promising approach for microalgae preservation. Thus, the objective of this study was to apply poly (lactic acid) nanofibers in the preservation of microalgae <em>Chlorella fusca</em> LEB 111 cells. The nanofibers were characterized regarding their morphology, thermal properties, structural characteristics and wettability. The microalgae cells were immobilized on the nanofibers and stored for 30 days at room temperature, refrigeration and thermostated chamber. Free microalgae cells were also maintained for the same period under the same conditions of the traditional method of microalgae preservation, continuous replication. The cell viability of the free and immobilized cells on the nanofibers was analyzed by Neutral Red (NR) and Trypan Blue (TB). At the end of the experiment, the immobilized cells showed greater viability (94 and 100 %) compared to the free cells (84 %). The cultivation of immobilized cells showed significant cell growth on the 25th day of cultivation for the evaluated storage conditions (3.6, 3.6 and 2.8 g L⁻¹ for refrigeration, room temperature and thermostatted chamber, respectively). Therefore, poly (lactic acid) nanofibers (PLA) are characterized as an innovative technology for microalgae maintenance.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103781"},"PeriodicalIF":4.6,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The determining regions, specific genes and markers of sex in Pyropia haitanensis (Bangiales, Rhodophyta)","authors":"Long-chen Tang ,&nbsp;Hong-chang Ding ,&nbsp;Ye-fan Qiu,&nbsp;Xing-hong Yan","doi":"10.1016/j.algal.2024.103780","DOIUrl":"10.1016/j.algal.2024.103780","url":null,"abstract":"<div><div>Genetic studies have confirmed that the sex of <em>Pyropia haitanensis</em> belongs to the genotypic determination. However, the genes involved in the sex determination have not yet been studied. In this study, two double haploid (DH) populations of the male and female were established using the sex-sectored blades produced by a crossing experiment of <em>P. haitanensis</em>, and the sex-linked regions (SLRs) were identified by BSA-Seq, which were located on chromosome 4. HiFi sequencing was performed on the SLRs of female and male for reassembly and gene annotations, and the results showed that the SLRs of female and male contained 93 and 94 genes with sizes of 0.32 and 0.31 Mb, respectively. In the SLRs of female and male, there was a sex-determining region (SDR) containing 5 and 3 genes, respectively. In addition, gene conversion of the SDRs resulted in non-Mendelian genetic segregation with a segregation ratio of male: female to be 4:0, 3:1, 1:3 or 0:4. Two female-specific genes (<em>PhF00071</em> and <em>PhF00072</em>) were found in the female SDR. Gene cloning revealed that the full-length CDS of <em>PhF00071</em> was 816 bp, encoding a protein containing an RWP-RK domain; while the full-length CDS of <em>PhF00072</em> was 858 bp, encoding a protein that did not contain any known domains. qRT-PCR revealed that the expression levels of the two female-specific genes were significantly increased during the formation of female germ cells (<em>P</em> &lt; 0.05). In addition, primers were designed using the specific sequences of female and male SDRs, and the genomic DNA of female, male and heterozygous strains were amplified by PCR. The results proved that only one specific band could appear in female or male strains, while both female and male-specific bands appeared in heterozygous strains. This study lays a foundation for further elucidating the mechanisms of sex determination and sexual maturity in <em>P. haitanensis</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103780"},"PeriodicalIF":4.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of low-dose X-ray radiation on the lipidome of Chlorella vulgaris","authors":"Mattia Casula ,&nbsp;Giacomo Fais ,&nbsp;Debora Dessì ,&nbsp;Cristina Manis ,&nbsp;Alessandra Bernardini ,&nbsp;Cyprien Verseux ,&nbsp;Viviana Fanti ,&nbsp;Pierluigi Caboni ,&nbsp;Giacomo Cao ,&nbsp;Alessandro Concas","doi":"10.1016/j.algal.2024.103783","DOIUrl":"10.1016/j.algal.2024.103783","url":null,"abstract":"<div><div><em>C. vulgaris</em> is a microalga with great potential as a source of lipids and essential fatty acids for human nutrition during extended space missions to Mars. However, the effects of Mars-like radiation on lipid composition are still poorly understood. In this study, we analyzed the effects of X-rays on the growth and lipid biosynthesis of <em>C. vulgaris</em> CCALA 269, exposing the cultures to doses of 450, 900, 1800, 3600, and 10,800 mSv, simulating approximately 354, 709, 1417, 2835, and 8504 days of exposure to Martian radiation, respectively. The results show that, although growth remained stable, doses exceeding 1800 mSv led to an increased production of specific lipid classes, suggesting an adaptive mechanism to counteract radiation stress. This adaptation was accompanied by an increase in reactive oxygen species (ROS) and changes in pigment composition, with an elevation in pheophytin-a and chlorophyll-a, and a decrease in chlorophyll-b. Our results demonstrate the ability of <em>C. vulgaris</em> to adapt to ionizing radiation, highlighting its suitability for sustainable lipid production in extraterrestrial environments, supporting human life on Mars through in situ resource utilization.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103783"},"PeriodicalIF":4.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macroalgae and microalga blend in dogs' food: Effects on palatability, digestibility, and fecal metabolites and microbiota","authors":"Cátia S.C. Mota ,&nbsp;Ana R.J. Cabrita ,&nbsp;Timur Yergaliyev ,&nbsp;Amélia Camarinha-Silva ,&nbsp;Agostinho Almeida ,&nbsp;Helena Abreu ,&nbsp;Joana Silva ,&nbsp;António J.M. Fonseca ,&nbsp;Margarida R.G. Maia","doi":"10.1016/j.algal.2024.103775","DOIUrl":"10.1016/j.algal.2024.103775","url":null,"abstract":"<div><div>The promotion of dogs' nutrition, health, and well-being are highly valued by pet owners, leading to an increasing interest in pet food with alternative, functional, and more sustainable supplements, such as algae. Few studies have assessed the supplementation of dog food with individual algae species, but no information exists on combined macro- and microalgae. This study evaluated the effects of dietary supplementation of a commercial algal blend, composed of two macroalgae (<em>Ulva rigida</em> and <em>Fucus vesiculosus</em>) and one microalga (<em>Chlorella vulgaris</em>) on palatability, intake, digestibility, metabolizable energy (ME), and fecal characteristics, metabolites, and microbiota of adult healthy Beagle dogs. Palatability was assessed by comparing a complete diet without (control) and with 1.5 % algae blend using twelve dogs. Then, six of these dogs were randomly selected for the digestibility trials. Following an initial trial to determine the control diet digestibility, a replicated Latin square was performed with three experimental periods of 10 days each, and three algal blend supplementation levels (0.5, 1.0, and 1.5 %). Dietary algal blend did not affect the first approach and first taste, but dogs preferred the control diet. Algal blend levels had no impact on intake, but organic matter, fiber, and energy digestibility increased with 1.5 % inclusion compared with 0.5 %. At the highest level, algae blend promoted fecal total short chain fatty acids and acetate while at lowest fecal propionate decreased and fecal production increased. Compared with the control diet, algal blend-supplemented diets promoted most nutrients and energy digestibility, diet ME content, and fecal quality, while reducing fecal butyrate. Fecal microbiota diversity and abundance were mostly unaffected by algae blend supplementation, with health-promoting genera <em>Turicibacter</em> and <em>Blautia</em> being the most abundant in all samples. Overall, results suggest algal blend as a promising alternative supplement for dog food, but further research is needed to unveil potential health-promoting effects.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103775"},"PeriodicalIF":4.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of seeding twine type and seeding density on hatchery performance and initial at-sea cultivation performance of the kelp Ecklonia radiata","authors":"Rebecca J. Lawton,&nbsp;Marie Magnusson","doi":"10.1016/j.algal.2024.103777","DOIUrl":"10.1016/j.algal.2024.103777","url":null,"abstract":"<div><div>Optimisation of cultivation methods is crucial to enable development of an efficient and cost-effective industry for novel seaweed species. Therefore, we investigated the effects of seeding twine type and zoospore seeding density on hatchery performance and initial at-sea cultivation performance of the emerging kelp aquaculture target <em>Ecklonia radiata</em>. Spores were seeded at three densities (2500, 5000 and 10,000 spores mL⁻¹) onto three twine types with differing diameters (Kuralon™: ⌀ 1.2 and 2 mm, nylon: ⌀ 1 and 3 mm, polyester: ⌀ 0.9 and 2 mm) and maintained in the hatchery for 60 or 74 days before outplanting to a marine farm site. At the end of the hatchery period sporophyte length and overall spool quality were highest on polyester and Kuralon™ seeding twines compared to nylon twines and sporophyte density was highest on polyester seeding twine, but two weeks after outplanting there were few differences between twines. Both hatchery performance and initial at-sea cultivation performance were best at seeding densities of 5000 and 10,000 spores mL⁻¹ compared to 2500 spores mL⁻¹ for twines with a 60-day hatchery period, but there were few differences between seeding densities for twines with a 74-day hatchery period. Seeding twine diameter did not have any significant effects on hatchery performance or initial at-sea cultivation performance. We recommend using a seeding density of 2500 spores mL⁻¹ and polyester or kuralon twine with a small diameter (e.g., ~1 mm) for cultivation of <em>E. radiata</em> to maximise efficiency and performance in the hatchery. Future studies should compare at-sea cultivation performance over an entire growing season to assess any effects of twine type on final biomass yield. Contrasts in our results with those of twine selection studies for other seaweeds highlight the importance of conducting species specific optimisation when developing cultivation techniques for new target species.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103777"},"PeriodicalIF":4.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scenedesmus subspicatus potential for pharmacological compounds removal from aqueous media","authors":"Fábio Rodrigo de Oliveira ,&nbsp;Tiele Medianeira Rizzetti ,&nbsp;Renato Zanella ,&nbsp;Julia Antunes de Oliveira ,&nbsp;Camila Rafaela Rathke ,&nbsp;Valéria Louzada Leal Butzke ,&nbsp;Ênio Leandro Machado ,&nbsp;Carlos Alexandre Lutterbeck ,&nbsp;Rosana de Cassia de Souza Schneider","doi":"10.1016/j.algal.2024.103771","DOIUrl":"10.1016/j.algal.2024.103771","url":null,"abstract":"<div><div>Emerging pollutants such as active pharmaceutical compounds (APCs) excreted by humans and animals are of growing concern due to their environmental impacts. This study aimed at to assess the phycoremediation potential of the microalgae <em>Scenedesmus subspicatus</em> for the removal of APCs, from water under controlled light and temperature conditions. The effects of hydrolysis and photolysis on the compounds were also monitored. Known concentrations of acetaminophen, acetylsalicylic acid, salicylic acid, albendazole, atenolol, propranolol, caffeine, carbamazepine, ibuprofen, ciprofloxacin, norfloxacin, ofloxacin, sulfamethoxazole, and trimethoprim were added in the microalgae medium. Samples were collected at 7-day intervals over twenty-one days and analyzed by UHPLC-MS/MS. Salicylic acid, albendazole, acetaminophen, atenolol, propranolol, and sulfamethoxazole were degraded by photolysis, hydrolysis, and phycoremediation, with the latter proving be more efficient. Propranolol and sulfamethoxazole presented low degradation by photolysis and hydrolysis. Some of the investigated compounds showed limited degradation and were not eliminated by any methods. Caffeine, carbamazepine, and trimethoprim were unaffected by hydrolysis, photolysis, or phycoremediation. Microalgae growth during the experiment was limited, suggesting toxic effects of some APCs. The findings highlight the importance of phycoremediation as a promising alternative for removing emerging pollutants from water.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103771"},"PeriodicalIF":4.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acid preservation of Laminaria hyperborea with formic and acetic acid and its effects on alginate","authors":"Katharina Nøkling-Eide ,&nbsp;Finn Lillelund Aachmann ,&nbsp;Kjell Josefsen ,&nbsp;Ariadna Szczybelski Ciordia ,&nbsp;Håvard Sletta ,&nbsp;Øystein Arlov","doi":"10.1016/j.algal.2024.103776","DOIUrl":"10.1016/j.algal.2024.103776","url":null,"abstract":"<div><div>Each year, 150,000 tons of <em>Laminaria hyperborea</em> is harvested commercially for alginate production in Norway. Traditionally, formalin has been an attractive preservative in the industry because it inhibits microbial growth and prevents alginate discolouration by cross-binding with polyphenols. However, due to potential health risks by handling formalin in large quantities, EU is restricting its use, and it is necessary to look for alternative preservation methods. Low molecular weight organic acids are used as food preservatives because they inhibit microbial growth when undissociated. The concentration of undissociated acid is a function pH, acid concentration and p<em>K</em>_a of the organic acid, hence different organic acids will have varied preservative efficiency at a given pH. This study investigated how preservation with formic acid (p<em>K</em>_a = 3.75) and acetic acid (p<em>K</em>_a = 4.76), affected the colour, yield, molecular weight, and composition of alginates from <em>L. hyperborea</em> stipe and lamina when stored at pH 3.7/4.7, 13/20 °C, and 2/8 weeks. The results were compared with formalin preservation (0.1 % formaldehyde). Microbial growth was prevented with both acids at pH 3.7, however, acid hydrolysis led to alginate depolymerisation. Acetic acid at pH 4.7 inhibited microbial growth in stipe biomass for 8 weeks, and when stored at 13 °C, the alginate molecular weight remained high (M_w = 426 kDa). Furthermore, acid preservation led to increased alginate yield and colour reduction compared with fresh and non-preserved biomass. Microbial growth in non-preserved samples led to enzymatic depolymerisation of alginates, resulting in a higher fraction and block length of guluronic acid.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103776"},"PeriodicalIF":4.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovering oxidized polar lipids in microalgae lipidome using liquid chromatography mass spectrometry approaches","authors":"Tiago Conde ,&nbsp;Diana Lopes ,&nbsp;Rita Pais ,&nbsp;Joana Batista ,&nbsp;Tatiana Maurício ,&nbsp;Felisa Rey ,&nbsp;Tânia Melo ,&nbsp;Pedro Domingues ,&nbsp;Rosário Domingues","doi":"10.1016/j.algal.2024.103764","DOIUrl":"10.1016/j.algal.2024.103764","url":null,"abstract":"<div><div>Microalgae are rich in polar lipids, such as glycolipids, phospholipids and betaine lipids esterified with omega-3 polyunsaturated fatty acids (PUFA), which are prone to oxidation. Under stress conditions, the redox balance in microalgae is altered, leading to an abnormal production of reactive oxygen species (ROS) that can affect surrounding lipids. While few oxidized polar lipids have been described to play important roles in mammals and possess interesting bioactive properties, their occurrence in microalgae is poorly described, hindering their exploitation as novel bioactive compounds. To enhance our understanding of these lipids, we conducted a targeted analysis of oxidized polar lipids in lipid extracts obtained from five microalgae species: <em>Chlorella vulgaris</em>, <em>Chlorococcum amblystomatis</em>, <em>Scendesmus obliquus</em>, <em>Nanochloropsis oceanica</em> and <em>Phaeodactylum ticornutum,</em> using reverse-phase liquid chromatography mass spectrometry (RP-LC-MS). A detailed analysis of the LC-MS data identified 150 oxidized polar lipid species across different classes of phospholipids, glycolipids and betaine lipids in the five microalgae species. These modified oxidized lipids featured oxygenated species with 1–3 additional oxygen atoms in the fatty acyl chains. The predominant oxidized fatty acids esterified to these lipids were omega-3 eicosapentaenoic acid (EPA, 20:5 <em>n</em>-3) and α-linonelic acid (ALA, 18:3 <em>n</em>-3). Notably, most oxidized lipid species were identified in diacylglyceryl-<em>N</em>,<em>N</em>,<em>N</em>-trimethylhomoserine (DGTS) in <em>C. amblystomatis, S. obliquus</em> and <em>N. oceanica</em>, monogalactosyldiacylglycerol (MGDG) in <em>P. tricornutum</em>, and phosphatidylethanolamine (PE) in <em>C. vulgaris</em>. Furthermore, distinct fragmentation patterns across lipid classes allowed the unequivocal identification of oxidized polar lipids. These findings reveal a diverse array of oxidized polar lipids in microalgae, predominantly enriched with oxidized omega-3 PUFA, highlighting microalgae as a natural source of oxidized polar lipids that may serve as a natural reservoir of bioactive omega-3 oxylipins.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103764"},"PeriodicalIF":4.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and performance of microalgae-based symbiotic systems for high-strength chemical oxygen demand wastewater treatment from the sugar mills","authors":"Siphelele Sibisi,&nbsp;Trisha Mogany,&nbsp;Faizal Bux,&nbsp;Ismail Rawat","doi":"10.1016/j.algal.2024.103773","DOIUrl":"10.1016/j.algal.2024.103773","url":null,"abstract":"<div><div>Agricultural and agro-industrial activities have risen exponentially to meet the ever-growing demand for food, energy, and other important resources. High freshwater consumption occurs in these sectors and is discharged as effluent containing excessive organic loads that require treatment. In this study, microalgal, bacterial, and fungal (yeast) isolates native to the sugar industry effluent were screened for effective chemical oxygen demand (COD) removal from wastewater when co-cultured. The microalgae-bacteria consortium (MBC) comprised <em>Chlorella sorokiniana</em> A7 and three bacterial strains including <em>Rhodococcus</em> sp. B009, <em>Bacillus</em> sp. B010, and B013; whilst the microalgae-yeast consortium (MYC) consisted of <em>Chlorella sorokiniana</em> A7 and <em>Saccharomyces cerevisiae</em> Y2. When the <em>Chlorella sorokiniana</em>-based symbiotic systems were characterized in sugar industry wastewater, excellent COD removal efficiencies were achieved compared to the axenic <em>Chlorella sorokiniana</em> A7. The COD removal efficiencies were 86 %, and 71 % after 96 h of cultivation for MBC, and MYC, respectively. After 168 h of cultivation in wastewater, ≥90 % of COD removal efficiency was observed in both MBC and MYC systems. The MYC also showed improved chlorophyll-<em>a</em> content, photosynthesis, and respiration in <em>Chlorella sorokiniana</em> A7. This study has demonstrated the efficiency of <em>Chlorella sorokiniana</em>-based consortium systems that could be used as eco-friendly and sustainable bioremediation tools for high-strength COD wastewater streams. An insight into mechanisms of interactions between <em>Chlorella</em> sp., and co-cultured microbial strains grown in sugar industry wastewater still needs further studies.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103773"},"PeriodicalIF":4.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}