Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Unveiling the potential of freshwater filamentous algae as a promising alternative source of essential fatty acids and bioactive carotenoids","authors":"Atiqur Rahaman,&nbsp;Dieter Hanelt,&nbsp;Klaus von Schwartzenberg,&nbsp;Abdelfatah Abomohra","doi":"10.1016/j.algal.2025.104100","DOIUrl":"10.1016/j.algal.2025.104100","url":null,"abstract":"<div><div>Developing a sustainable and cost-effective food source enriched with essential fatty acids (EFAs) and other bioactive compounds, while minimizing the environmental impact, remains a significant challenge nowadays. In that regard, filamentous algae can be harvested more easily and at a lower cost compared to unicellular microalgae, making large-scale production of biomass and bioactive compounds more eco-friendly. The present study explores the potential of freshwater filamentous algae as a promising source for value-added products, particularly EFAs and bioactive pigments. The study involved an initial screening of 91 filamentous algae strains from the Microalgae and Zygnematophyceae Collection Hamburg (MZCH) to identify isolates with high growth rate. Eleven promising candidates were selected for further biochemical evaluation. Cell dimensions showed 5.8–117.2 μm length and 6.4–56.7 μm width. This variability in cell size enhances their suitability for specific applications, such as textile industry, where larger filaments can be directly utilized in producing bio-based fibers, yarns and fabrics. Among the studied algae, <em>Oedogonium foveolatum</em> and <em>Spirogyra</em> sp. #771 showed the highest biomass concentration (dry weight) of 0.70 and 0.67 g L⁻¹, respectively. Regarding value-added products, <em>Hyalotheca dissiliens</em> showed the highest carbohydrate content of 56.4 ± 2.32 % of dry weight (dw%), while <em>Mougeotiopsis calospora</em> showed the highest protein content of 48.0 ± 2.19 dw%. <em>Klebsormidum nitens</em> showed the highest total bioactive carotenoids (tBC) content of 11.5 ± 0.05 mg g⁻¹ dw and the highest tBC concentration of 4.03 ± 0.16 mg L⁻¹. Interestingly, <em>Groenbladia neglecta</em> stood out with the highest recorded lipid content of 34.4 ± 1.59 dw% and EFAs content of 14.3 ± 0.46 dw%. It also achieved a balanced ω-6/ω-3 ratio of 3.57 ± 0.03, which is optimal for dietary applications. The present study suggests <em>G. neglecta</em> under the mentioned growth conditions as a promising candidate for developing sustainable EFA-rich food sources, warranting further research and development.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104100"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144137690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alkaline phosphatase-coupled high affinity phosphate transporters were involved in low phosphorus stress responding in Pyropia yezoensis","authors":"Xianrong Gao ,&nbsp;Chao Tian ,&nbsp;Shurui Wang ,&nbsp;Menglin Guo ,&nbsp;Jianfeng Niu ,&nbsp;Guangce Wang","doi":"10.1016/j.algal.2025.104101","DOIUrl":"10.1016/j.algal.2025.104101","url":null,"abstract":"<div><div>Phosphate (Pi) often becomes a limiting nutrient in oceans, particularly in macroalgae aquaculture areas. More importantly, the continuous increase of <em>Pyropia yezoensis</em> cultivation density has exacerbated Pi deficiency issues. This study used transcriptome analysis of <em>Py. yezoensis</em> seedlings under low-Pi conditions and identified the gene <em>PyALP</em>, an alkaline phosphatase, which was significantly upregulated. Through Weighted Gene Co-expression Network Analysis (WGCNA) and Pearson correlation, <em>PyPHO89</em>, a Pi transporter gene, and six transcription factors were co-expressed with <em>PyALP</em>. Bioinformatics predictions indicated that PyALP belongs to a PhoA type enzyme. However, the molecular structures of the glycosylphosphatidylinositol (GPI) and anchor the N-terminal glycine were not detected, suggesting that PyALP is confined to the microenvironment between the cell wall and plasma membrane, where it hydrolyzes dissolved organic phosphorus (DOP). PyPHO89 was predicted to be a high-affinity symporter for inorganic phosphorus uptake. Promoter analysis revealed Myb binding sites in both <em>PyALP</em> and <em>PyPHO89</em>. Among the screened potential transcription factor genes, only <em>Py08914</em> possessed a conserved SHLQKYR motif and a glutamine-rich C-terminal sequence, which are characteristic of a typical Pi deficiency-responsive transcription factor. Quantitative RT-PCR showed upregulation of <em>PyALP</em> and <em>PyPHO89</em> after 6 h of Pi-starvation, and expression of <em>Py08914</em> peaked in the first 2–4 h of starvation. This implies that the hydrolysis of DOP and uptake of phosphorus are co-regulated by the Myb transcription factor. PyALP activity increased with Pi-starvation and both phosphoesters and phosphonates could be used with similar efficiency. This is the first report regarding the Pi-deficiency response mechanism in <em>Py. yezoensis</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104101"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of cultivar selection, cultivation region and harvest timing on the structure and properties of carbohydrates from Saccharina japonica","authors":"Mingjie Tian ,&nbsp;Yueqi Kong ,&nbsp;Delin Duan ,&nbsp;Soon-Mi Shim ,&nbsp;Xiaoting Fu","doi":"10.1016/j.algal.2025.104106","DOIUrl":"10.1016/j.algal.2025.104106","url":null,"abstract":"<div><div>The study aimed to explore the difference of yields, structures, and functions of three carbohydrates in <em>Saccharina japonica</em> (<em>S. japonica</em>), considering the differences of cultivars of Haiza (the northern cultivar) and Puke (the southern cultivar), cultivation regions (the north, the south, and the transplanted Puke from south to north), and harvesting times during their whole cultivation. The yield of mannitol was significantly higher in samples cultivated in the north region (5.07–27.82 %) compared to those from the south (2.34–3.37 %), and for both cultivars the optimal yield was in May and June. The molecular weight of alginate was lower in Puke but higher in both Haiza and the transplanted Puke. The mannuronate/guluronate ratio of alginate noticeably deceased from levels of 2.28–2.40 to levels of 1.68–1.93 after transplantation Puke from the south to north, which indicated the possible upregulation of the expression of genes encoding mannuronate C5-epimerases in colder temperature cultivation. Meanwhile, different fucoidan samples showed diverse monosaccharide composition, sulfate content, sulfation positions, and antioxidant activities. Fucoidans from Haiza showed better ABTS and hydroxyl radical scavenging ability. The correlation analysis indicated that the fucose content was positively correlated with ABTS radical scavenging ability (<em>R</em> = 0.576), while fucose and sulfate group contents were negatively correlated with the hydroxyl radical scavenging ability (<em>R</em> = 0.581 and <em>R</em> = 0.829). Overall, this study deepened the understanding of carbohydrates diversity in <em>S. japonica</em> and provided a scientific foundation for optimizing its cultivation strategies.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104106"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The combined effect of phosphorus forms and zinc on arsenic toxicity and bioaccumulation in Phaeodactylum tricornutum","authors":"Jiandi Li ,&nbsp;Liping Jia ,&nbsp;Yongqiang Tian ,&nbsp;Xiaohuang Chen ,&nbsp;Fengjiao Liu ,&nbsp;Xuguang Huang","doi":"10.1016/j.algal.2025.104097","DOIUrl":"10.1016/j.algal.2025.104097","url":null,"abstract":"<div><div>Microalgae employ transporter proteins that are shared arsenate (As(V)) to efficiently uptake phosphate (dissolved inorganic phosphorus, DIP). Additionally, zinc-activated enzymes, alkaline phosphatase (AP), drive the dissolved organic phosphorus (DOP) assimilation, thereby intricately linking the dynamics of zinc (Zn), phosphorus (P), and As(V). This study investigated the effects of two distinct P forms (DOP and DIP) and Zn levels on the toxicity and bioaccumulation of As(V) in <em>Phaeodactylum tricornutum</em>. The results showed that <em>P. tricornutum</em> showed a marked decrease in chlorophyll <em>a</em> (Chl <em>a</em>), maximal photochemical efficiency of Photosystem II (Fv/fm) parameter for plant stress detection, and cell density in DOP cultures compared with DIP cultures. However, the supplementation of Zn alleviated the development of <em>P. tricornutum</em> in DOP conditions. Furthermore, the results also indicated that the 96-h EC₅₀ values of As(V) toxicity in <em>P. tricornutum</em> was enhanced by DOP, but the addition of Zn alleviates this effect. Moreover, the intracellular As of <em>P. tricornutum</em> was reduced by 35.79 %, and 36.80 % in DOP+Zn culture compared to DOP culture at 0.05, and 0.1 μmol·L⁻¹ As(V) exposures, respectively. However, there were no significant differences between Zn additions to the growth and intracellular As of <em>P. tricornutum</em> in DIP conditions. These results suggest that DOP enhances As toxicity in microalgae, but Zn can significantly attenuate the toxicity and bioaccumulation of As in microalgae under DOP, thereby impacting the cycling of As in marine ecosystems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104097"},"PeriodicalIF":4.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of heavy metal tolerance and lipid accumulation in Euglena gracilis under Cd stress by γ-aminobutyric acid: A physiological, biochemical, and metabolomic study","authors":"Chao Li ,&nbsp;Ke Xu ,&nbsp;Mengzhe Du ,&nbsp;Ming Du ,&nbsp;Zixi Chen ,&nbsp;Qiong Liu ,&nbsp;Hui Zhu ,&nbsp;Liqing Zhao ,&nbsp;Jiangxin Wang","doi":"10.1016/j.algal.2025.104098","DOIUrl":"10.1016/j.algal.2025.104098","url":null,"abstract":"<div><div><em>Euglena gracilis</em> (<em>E. gracilis</em>) is a unicellular eukaryotic photosynthetic protist without cell wall. As a model organism, it has been extensively studied in endosymbiosis, chloroplast development, environmental bioremediation, and high-value product synthesis. Heavy metal pollution, such as cadmium (Cd) stress, is prevalent and induces high levels of reactive oxygen species (ROS) in microalgae, leading to oxidative stress, reduced photosynthetic efficiency, and inhibited growth. This study explored the application of γ-aminobutyric acid (GABA), a plant hormone-like substance, to enhance heavy metal tolerance in <em>E. gracilis</em> under Cd stress, aiming to increase the accumulation of high-value by-products such as lipids. As previously studied, our findings also showed that Cd stress significantly inhibited <em>E. gracilis</em> growth and pigment accumulation. However, it is worth noting that our results showed that exogenous GABA addition improved cell counts, lipid content, protein content and pigment content. GABA also significantly increased glutathione (GSH) content. Notably, Metabolomic analysis revealed that Cd stress shifted <em>E. gracilis</em> energy storage from carbohydrates to lipids and proteins, and exogenous GABA addition further enhanced this trend. These results suggest that GABA play a significant role in increasing lipid accumulation and enhancing Cd tolerance in <em>E. gracilis</em>, providing an economical and eco-friendly strategy for lipid production and Cd adsorption.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104098"},"PeriodicalIF":4.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pp07886, a glycoside hydrolase family 5 protein from Pythium porphyrae, is a required virulence factor and induces host disease resistance","authors":"Huichao Yang ,&nbsp;Zhaolan Mo ,&nbsp;Peiwen Weng ,&nbsp;Yongwei Yan ,&nbsp;Lei Tang ,&nbsp;Xiaojun Rong ,&nbsp;Jie Li","doi":"10.1016/j.algal.2025.104096","DOIUrl":"10.1016/j.algal.2025.104096","url":null,"abstract":"<div><div><em>Pythium porphyrae</em> is an important waterborne oomycete pathogen causing red rot disease in the red alga <em>Pyropia yezoensis</em>. However, the virulence factors and pathogenic mechanisms of <em>Pyt</em>. <em>porphyrae</em> remain unknown, hindering the development of prevention and control methods against red rot disease. In this study, the <em>Phytophthora sojae</em> transformation system was used to investigate the virulence factors – glycoside hydrolase family 5 (GH5) in <em>Pyt</em>. <em>porphyrae</em>. The expression of 14 GH5 genes in <em>Pyt</em>. <em>porphyrae</em> at different developmental and infection stages was analyzed. The GH5 gene <em>Pp07886</em> was significantly induced at the early (30 min post-infection) and middle (5 days post-infection) stages of infection, indicating its critical role in <em>Pyt</em>. <em>porphyrae</em> invasion. Heterologous expression transformant of <em>Phy</em>. <em>sojae</em> (Ps-hePp07886-11) and mutants of <em>Ps4041</em> (the homolog of <em>Pp07886</em> in <em>Phy</em>. <em>sojae</em>), PsΔ4041-RFP and PsΔ4041-Pp07886, were generated to evaluated the function of <em>Pp07886</em>. Compared to the wild-type strain, the virulence of Ps-hePp07886-11 increased significantly in soybeans. Compared to PsΔ4041-RFP, the mycelial growth and virulence of PsΔ4041-Pp07886 enhanced. These results indicated that <em>Pp07886</em> may contribute to growth and virulence. The qRT-PCR and immunoblotting revealed rapid induction of <em>Pp07886</em> within 3 h post-infection in both Ps-hePp07886-11 and PsΔ4041-Pp07886. Furthermore, the recombinant Pp07886 protein could improve the resistance of soybean to <em>Phy</em>. <em>sojae</em> P6497 infection. These results suggested that Pp07886 may play an important role in oomycete virulence and growth, and could enhance the host resistance to pathogen infection.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104096"},"PeriodicalIF":4.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response mechanisms of microalgal-bacterial granular sludge to different loading strategies of copper oxide nanoparticles","authors":"Alfonz Kedves ,&nbsp;Zoltán Kónya","doi":"10.1016/j.algal.2025.104099","DOIUrl":"10.1016/j.algal.2025.104099","url":null,"abstract":"<div><div>This study investigated the effects of copper oxide nanoparticles (CuO NPs) at concentrations of 1–50 mg/L on the microalgal-bacterial granular sludge (MBGS) wastewater treatment process, comparing two nanoparticle addition methods: shock-load (SL) and stepwise (SW) increases. The results demonstrated that the chemical oxygen demand (COD) and ammonia‑nitrogen (NH₃-N) removal were more sensitive in the reactor with shock-load (R_SL), phosphate concentrations were higher in the effluent from the reactor with stepwise increases (R_SW). Accumulation of nitrate‑nitrogen was observed only in the SW method at CuO NP concentrations ≥20 mg/L, likely due to the nanoparticles becoming entrapped within the granule interiors, as evidenced by scanning electron microscopy coupled with energy-dispersive X-ray (SEM-EDX) mapping. Although total EPS levels were highest in both reactors at 20 mg/L CuO NP dosage, the levels were significantly higher in the RSW sludge (191.1 mg/g MLVSS in R_SL vs. 218.3 mg/g MLVSS in R_SW), primarily due to the long-term exposure leading to increased loosely-bound EPS (LB-EPS) content (75.7 mg/g MLVSS in R_SL vs. 102.9 mg/g MLVSS in R_SW). Furthermore, CuO NPs interacted with EPS, binding to N<img>H, C<img>N, C<img>O, and C-O-C groups in protein (PN) and polysaccharide (PS) structures.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104099"},"PeriodicalIF":4.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stressing cyanobacteria for enhanced production of bulk and value-added chemicals","authors":"Guanlan Mo ,&nbsp;Xuejing Xu ,&nbsp;Huili Sun ,&nbsp;Jiahui Sun ,&nbsp;Shaoming Mao ,&nbsp;Jinyu Cui ,&nbsp;Guodong Luan ,&nbsp;Xuefeng Lu","doi":"10.1016/j.algal.2025.104095","DOIUrl":"10.1016/j.algal.2025.104095","url":null,"abstract":"<div><div>Cyanobacteria, ancient photosynthetic prokaryotes capable of directly converting CO₂ and solar energy into various chemicals through photosynthesis, have drawn great attention as a photosynthetic biomanufacturing platform. To enhance the production of various chemicals, multi-dimensional engineering strategies spanning genetic modification to process optimization have been developed. Notably, stress activation strategies, characterized by their strong controllability and the absence of transgenic risks, have recently attracted significant attention. These strategies could reprogram native metabolic networks, effectively redirecting photosynthetic flux toward targeted biosynthesis pathways while maintaining cellular viability, thereby boosting the production of both bulk and value-added chemicals. This review summarizes recent advances in stress-driven cyanobacterial chemical production, including compatible solutes, pigments and biofuels. We dissect regulatory mechanisms at transcriptional, translational, and enzymatic levels, and discuss challenges and potential strategies for enhancing the large-scale industrial production of cyanobacteria under stress conditions.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104095"},"PeriodicalIF":4.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine algae oligosaccharides: Eco-friendly elicitors for sustainable agriculture","authors":"Abhisha Roy ,&nbsp;Girish T.R. ,&nbsp;Sri Sailaja Nori ,&nbsp;Shrikumar Surya Narayan ,&nbsp;Saikat Bhattacharjee ,&nbsp;Vipin Hallan","doi":"10.1016/j.algal.2025.104093","DOIUrl":"10.1016/j.algal.2025.104093","url":null,"abstract":"<div><div>With an increasing demand for agricultural production to meet global needs, the emphasis is on enhancing crop yields over expansion on arable farming lands. Traditional methods to combat plant diseases involving fungicides, bactericides, and insecticides have raised concerns due to their adverse effects on health and the environment. Consequently, there is an urgent need for novel and environmentally friendly approaches to control plant diseases. Elicitors are defined as substances that stimulate the production of specific bioactive components. These compounds, when applied in small quantities, activate plant defense mechanisms. Seaweeds, classified into three major groups, play crucial roles in marine ecosystems and have great economic significance. Among the aquatic flora, these marine algae are especially notable for their high content of marine algae polysaccharides (MAP), including carrageenan, alginate, fucoidan, laminaran, agarose and ulvan that have been reported to act as elicitors of plant defenses. When hydrolyzed, MAPs yield marine algae oligosaccharides (MAOs), which garnered more significant interest in recent years because of their superior solubility. This review presents a holistic view of seaweeds as natural elicitors, primarily focusing on MAPs and MAOs in enhancing the defensive capabilities of plants. We discuss various methodologies deployed to process MAOs from MAPs, and provide a comprehensive comparison of their efficacies. Further, we explore their application regimens of as elicitors for plant defense, particularly in the context of sustainable agriculture.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104093"},"PeriodicalIF":4.6,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon dioxide-assisted enhancement of microalgae growth and pollutant removal in piggery wastewater by newly-isolated ammonia-tolerant microalgae Chlorella sorokinfana","authors":"Junjie He ,&nbsp;Jingcheng Zhang ,&nbsp;Huaiyi Ren ,&nbsp;Yao Zhang ,&nbsp;Huankai Li ,&nbsp;Hui Liu","doi":"10.1016/j.algal.2025.104094","DOIUrl":"10.1016/j.algal.2025.104094","url":null,"abstract":"<div><div>Livestock and poultry breeding produces a large amount of carbon dioxide (CO₂) and wastewater with high concentrations of ammonia‑nitrogen (NH₃−N), such as piggery wastewater (PW). Whether CO₂ can promote microalgae growth and pollutant removal is promising in the green and sustainable treatment of PW. Thus, this study isolated an ammonia-tolerance microalgae species from the PW, which was used to find optimal CO₂ aeration concentration in the microalgae-based PW treatment. The isolated species was identified as <em>Chlorella sorokinfana</em> (genetic similarity of 100 %). The optimal 20 % CO₂ addition could provide carbon sources and balance pH, when compared with the control group within nine days, the growth rate and chlorophyll <em>a</em> of the isolated microalgae reached 6.00 × 10⁷ cells/mL/d and 8.79 mg/L, which considerably increased by 113 % and 79 %, respectively (<em>p</em> &lt; 0.05); the removal efficiencies of chemical oxygen demand, dissolved organics, total nitrogen, NH₃-N, and total phosphorous were significantly increased from 42 %, 80 %, 23 %, 28 %, and 34 % to 70 %, 88 %, 66 %, 72 %, and 99 %, respectively (<em>p</em> &lt; 0.05). Differential expression genes were also the highest in the 20 % CO₂ vs the control groups. Based on the enrichment analysis, 20 % CO₂ aeration upregulated ribosome biogenesis and nitrogen metabolism, thus promoting microalgae growth and protein synthesis.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104094"},"PeriodicalIF":4.6,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}