Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Seaweed-mediated Ca-doped ZnO nanoparticles for sodic soil reclamation and enhanced growth of Arachis hypogaea L.","authors":"G. Keerthana,&nbsp;Mohammed Al-zharani,&nbsp;Mohammed Mubarak,&nbsp;Jayanthi Barasarathi,&nbsp;Mythili Ravichandran,&nbsp;R. Dineshkumar","doi":"10.1016/j.algal.2026.104981","DOIUrl":"10.1016/j.algal.2026.104981","url":null,"abstract":"<div><div>The present study, biogenic calcium-doped zinc oxide nanoparticles (Ca-ZnO NPs) were synthesized using calcium carbonate (CaCO₃) derived from the marine macroalgae <em>Halimeda macroloba</em> and evaluated for their potential in sodic soil reclamation and growth improvement of groundnut (<em>Arachis hypogaea</em> L.). The synthesized CaCO₃ and Ca-ZnO NPs were characterized using UV–vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA) for characterization and confirmation. A pot experiment was conducted to evaluate the effects of Ca-ZnO NPs on soil physicochemical properties and plant growth under sodic soil conditions to improve agricultural productivity. The application of Ca-ZnO NPs significantly improved seed germination (93.3%) compared with untreated sodic soil (66.7%). Plant growth parameters, including leaf number (5.3 plant⁻¹) and root length (5.6 cm), were higher in treated soil than in the control. Soil analysis revealed a reduction in soil pH from 9.8 ± 0.12 to 7.5 ± 0.08 and electrical conductivity from 7.6 ± 0.35 to 2.3 ± 0.21 mS cm⁻¹ following Ca-ZnO NPs application. Exchangeable sodium percentage decreased from 14.57 ± 0.80% to 7.03 ± 0.52% during the 20-day incubation period. Elemental analysis indicated increased concentrations of essential nutrients, particularly calcium (from 5.1 to 140 g kg⁻¹), along with reductions in toxic trace elements. This study presents a novel approach by utilizing seaweed-derived biogenic CaCO₃ as a sustainable calcium dopant for the green synthesis of ZnO nanoparticles, enabling simultaneous sodic soil amelioration and groundnut growth promotion through an eco-friendly nanotechnology-based strategy.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"99 ","pages":"Article 104981"},"PeriodicalIF":5.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149140616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon capture potential assessment of algal cultivation in China based on LCA","authors":"Mao Mu,&nbsp;Shiming Zhou,&nbsp;Shaozhen Hou,&nbsp;Feng Tan,&nbsp;Wanming Lu,&nbsp;Leyou Wang,&nbsp;Shuang Wang","doi":"10.1016/j.algal.2026.104990","DOIUrl":"10.1016/j.algal.2026.104990","url":null,"abstract":"<div><div>To address the overlooked effects of algal categories and geographical conditions on CO₂ fixation, this study evaluates the global warming potential (GWP) and net CO₂ capture of microalga (<em>Chlorella vulgaris</em>, <em>C. vulgaris</em>) and macroalga (<em>Enteromorpha prolifera</em>, <em>E. prolifera</em>) cultivation across China using life cycle assessment (LCA). First, spatially resolved growth models for both algae were developed to determine their nationwide productivity. Taking the province with maximum productivity as a reference, the contribution of subprocess to overall cultivation GWP and the geographical distribution of GWP across China were determined. Key findings indicate that artificial lighting (requiring electricity) in photobioreactors (PBR) is uneconomical. Replacing artificial lighting with sunlight could reduce the GWP of PBR cultivation below that of open raceway pond (ORP) cultivation. However, separation and drying processes in both <em>C. vulgaris</em> cultivation systems consume substantial electricity, significantly increasing GWP of overall process and thus reducing their net CO₂ capture. After optimizing separation and drying, PBR cultivation of <em>C. vulgaris</em> shows advantages over <em>C. vulgaris</em> cultivation in ORP and demonstrated a net CO₂ capture range from 164.4 g m⁻² year⁻¹ (Guangxi) to 2165.2 g m⁻² year⁻¹ (Yunnan); Nevertheless, net CO₂ capture of <em>C. vulgaris</em> remains smaller than that of <em>E. prolifera</em> (over 2500 g m⁻² year⁻¹). Furthermore, 25.37 million hectare <em>E. prolifera</em> cultivation shows potential of absorbing 1.64–2.26 billion ton CO₂ annually. Although this figure is equivalent to 15–21% of China's annual CO₂ emissions, it represents a theoretical upper-bound cultivation-stage capture potential under idealized assumptions, rather than realized climate mitigation. In addition, even proving that the tremendous algal reserve and carbon sink generated via initial CO₂ capture during cultivation pave the way for further downstream application, this study does not evaluate cradle-to-grave carbon sequestration, which could be accessed by further research.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"99 ","pages":"Article 104990"},"PeriodicalIF":5.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149140620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automating microalgal raceway pond operations using real-time spectroradiometric monitoring","authors":"Song Gao,&nbsp;Christopher Katinas,&nbsp;Jerilyn A. Timlin,&nbsp;Thomas A. Reichardt,&nbsp;Scott Edmundson,&nbsp;Michael Huesemann","doi":"10.1016/j.algal.2026.104706","DOIUrl":"10.1016/j.algal.2026.104706","url":null,"abstract":"<div><div>Biomass concentration is among the most important metrics in microalgal cultivation to track growth and inform operation. Sensors commonly used for biomass concentration measurements that involve direct interaction with the culture medium often face limitations such as biofouling in long-term applications. In this study, we successfully integrated noncontact spectroradiometric sensors to perform real-time, automatic dilution control in outdoor raceway ponds. By utilizing the sensor inputs, we demonstrated the successful implementation of two cultivation strategies in outdoor raceway ponds. For turbidostat operation, spectroradiometric sensors enabled automatic dilution control so that the biomass concentration could be consistently maintained near a predetermined set point. For the Optimized Illuminance strategy, light distribution was estimated in real time and used to minimize the dark zone through automatic dilution of the culture. In both cases, the dilution rate dynamically responded to varying weather, leading to substantial increases in biomass productivity—51% for turbidostat and 84% for Optimized Illuminance—compared with that of the semi-continuously diluted culture. Although challenges such as noise from low solar angles and a foamy culture surface affected sensor accuracy, the results demonstrate the potential of spectroradiometric sensing technologies to enhance algal productivity, reduce operational costs, and improve scalability for outdoor cultivation systems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"98 ","pages":"Article 104706"},"PeriodicalIF":5.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multifaceted anti-cancer strategy through apoptosis induction, MMP suppression, and anti-metastatic activity of bioactive pigment–rich lipophilic extracts and their chemical characterization from marine cyanobacteria isolates","authors":"Ghada E. Hegazy,&nbsp;Reham Gamal,&nbsp;Hussein Oraby,&nbsp;Esmail M. El-Fakharany","doi":"10.1016/j.algal.2026.104790","DOIUrl":"10.1016/j.algal.2026.104790","url":null,"abstract":"<div><div>This study investigated the isolation, chemical characterization, and anti-cancer potential of three cyanobacterial strains: <em>Oscillatoria simplicissima</em> Q1, <em>Oscillatoria acutissima</em> Q2, and <em>Phormidium formosum</em> Q3. Pigment-rich lipophilic extracts were analyzed using GC–MS and FT/IR, revealing the presence of diverse bioactive metabolites, including phytol, squalene, β-sitosterol, and linoleic acid, which are known for their anticancer properties. Biological evaluation demonstrated that all extracts exhibited selective cytotoxic activity against human carcinoma cell lines (A431, A549, and MDA), with high safety toward normal human dermal fibroblast (HSF) cells. The highest activity was observed for the Q3 extract against A549 cells, with an IC₅₀ value of 41.78 μg/mL and a corresponding selectivity index (SI) of 8.16, indicating strong selectivity toward cancer cells. Mechanistically, the observed anticancer activity is attributed to the combined action of key lipophilic metabolites such as phytol (apoptosis induction), β-sitosterol (cell proliferation inhibition), and squalene (oxidative stress modulation), supported by morphological evidence of apoptosis. Furthermore, the extracts significantly inhibited cancer cell migration, with Q3 showing up to 95.34% inhibition after 48 h, and downregulated MMP-2 and MMP-9 gene expression by up to 1.82-fold and 2. 32-fold, respectively. These findings highlight the potential of cyanobacterial pigment-rich lipophilic extracts as promising multi-target anticancer agents.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104790"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sourcing edible seaweeds for dietary supplements: a case study of fucoidans in the global market","authors":"Yuetuan Zhang,&nbsp;Maonian Xu,&nbsp;Olivia DeAngelis,&nbsp;Sankar Rathinam,&nbsp;Hildur Inga Sveinsdóttir,&nbsp;Björn Viðar Aðalbjörnsson","doi":"10.1016/j.algal.2026.104811","DOIUrl":"10.1016/j.algal.2026.104811","url":null,"abstract":"<div><div>Fucoidans are bioactive sulfated fucans in brown macroalgae, which have the potential for functional foods or pharmaceuticals. However, the fucoidan ingredients sold in the global market have varying quality. In this study, we aimed to characterize the quality of commercial fucoidan ingredients in the market by comparing them with two authentic fucoidan standards from <em>Fucus vesiculosus</em> and <em>Undaria pinnatifida</em>. The key constituting monosaccharide of fucoidan fucose as well as the monosaccharide profiles was determined using a high-performance liquid chromatography (HPLC) method. In addition, phenolic impurities, protein contents and sulfate contents in fucoidan samples were estimated using the Folin-Ciocalteu method, the Bradford method and the barium chloride-gelatin turbidimetric assay, respectively. Fourier-transform infrared spectroscopy (FTIR) was used for structural characterization of fucoidans, particularly the bands at 820–850 cm⁻¹ and 1225–1250 cm⁻¹ indicating the presence of sulfates. Out of eight commercial ingredients, three (i.e. s5, s6 and s8) did not have any fucose, sulfates or characteristic FTIR signals of fucoidans, indicating lack of fucoidans, while the rest mostly matched the standards of the same sourcing seaweed species. Phenolic impurities are in relatively low amount (10 to 124 μg/g), and the color intensity of commercial fucoidan samples (in powders) indicated that high extraction temperatures may increase the amount of co-extracted phenolics. Protein impurity contents are minor concerns in both analytical standards (&lt;0.5%) and commercial samples with the most less than 1%. However, all commercial samples showed significantly lower sulfate contents than their corresponding analytical standards. Our study highlights the quality concerns in the current global fucoidan market as well as the importance of comprehensive carbohydrate analyses for quality assurance.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104811"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Freeze-thaw cycles-assisted structural remodeling of soluble dietary fiber from Pyropia haitanensis: Multi-scale characterization and functional enhancement","authors":"Yujia Ou,&nbsp;Bingqian Gu,&nbsp;Minyan Chen,&nbsp;Shuang Tian,&nbsp;Tao Hong,&nbsp;Zedong Jiang,&nbsp;Hui Ni,&nbsp;Mingjing Zheng","doi":"10.1016/j.algal.2026.104787","DOIUrl":"10.1016/j.algal.2026.104787","url":null,"abstract":"<div><div>The effects of freeze-thaw cycles (FTC) on the structural and functional properties of soluble dietary fiber (SDF) extracted from <em>Pyropia haitanensis</em> were systematically investigated. Various FTC (1, 3, and 5 cycles) were applied to evaluate changes in SDF yield, microstructure, molecular conformation, thermal stability, physicochemical characteristics, and <em>in vitro</em> inhibitory activities against α-glucosidase and pancreatic lipase. The results showed that moderate FTC treatment, particularly three cycles, significantly increased the yield of SDF extracted from <em>P. haitanensis</em> to 22.94%, representing an approximate 14.59% increase compared to the control (<em>p</em> &lt; 0.05). Structural characterization revealed that FTC treatment transformed the compact SDF aggregates into a looser and more porous architecture, altered the O<img>H bonding environment, increased the accessibility of hydrophilic groups, and modulated crystalline order. LF-NMR analysis further indicated that FTC treatment changed water-state distribution, with FTC3 favoring water retention within the fiber matrix. Accordingly, FTC-treated SDF showed improved water-holding capacity, swelling capacity, and oil-holding capacity, with maximum values of 6.23 g/g, 10.39 mL/g, and 9.92 g/g, respectively. In addition, FTC-treated SDF exhibited enhanced inhibitory activities against α-glucosidase and pancreatic lipase, with maximum inhibition rates of 61.22% and 57.66%, respectively (<em>p</em> &lt; 0.05). These findings indicate that appropriate FTC treatment can improve the structure-related functionality of <em>P. haitanensis</em> SDF, providing a mild physical strategy for the high-value utilization of red-algal dietary fiber in functional food applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104787"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite-mediated enhancement of Synechococcus elongatus PCC 7942 growth by Rhodococcus sp. AF2108: Insights from stable isotope and transcriptomic analyses","authors":"Pei Yu Tan,&nbsp;Yuta Kato,&nbsp;Akihiro Hachikubo,&nbsp;Masaaki Konishi","doi":"10.1016/j.algal.2026.104803","DOIUrl":"10.1016/j.algal.2026.104803","url":null,"abstract":"<div><div>Cyanobacteria, which are promising platforms for sustainable bioproduction, are often limited by environmental and metabolic constraints under monoculture conditions. This study investigated the possible factors by which heterotrophic bacteria enhance cyanobacterial growth by co-culturing <em>Synechococcus elongatus</em> PCC 7942 and the following four cyanobacterium growth-promoting bacteria (CGPBs) in a membrane-separated Beppu flask system in a co-culture medium containing 5 g/L glucose to support heterotrophic bacterial activity: <em>Rhodococcus</em> sp. AF2108, <em>Ancylobacter</em> sp. GA1226, <em>Xanthobacter</em> sp. AF2111, and <em>Shewanella</em> sp. OR151. All CGPBs stimulated <em>S. elongatus</em> PCC 7942 growth, but AF2108 had the strongest effect by 11.5-fold increase of chlorophyll <em>a</em>. A stable isotope analysis revealed increases in δ¹³C and δ¹⁵N, indicating increased bicarbonate assimilation and nitrogen turnover. A transcriptomic analysis detected the upregulation of genes associated with the Calvin–Benson cycle, tricarboxylic acid cycle, glutamine synthetase–glutamate synthase cycle, and amino acid biosynthetic pathways along with the differential expression of iron-related ATP-binding cassette transporter genes. These results suggest that AF2108 promotes <em>S. elongatus</em> PCC 7942 growth through diffusible factors under glucose-containing co-culture conditions. These factors may include respiration-derived CO₂ and other unidentified bacterial metabolites, which together may influence photosynthesis, redox balance, and nutrient turnover. The study provides an exploratory model of non-contact cyanobacterium–bacterium interactions relevant to microbial consortia design.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104803"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of antimicrobial Defensins in diatom: Engineering Phaeodactylum tricornutum as a cell factory","authors":"Malika Mekhalfi,&nbsp;Eva Gourdon,&nbsp;Leanne Troussier,&nbsp;Phillipe Bulet","doi":"10.1016/j.algal.2026.104792","DOIUrl":"10.1016/j.algal.2026.104792","url":null,"abstract":"<div><div>The growing threat of antibiotic resistance requires new antimicrobial strategies. Antimicrobial peptides (AMPs), including β-Defensins, represent promising therapeutic candidates. This study evaluates the diatom <em>Phaeodactylum tricornutum</em> as a sustainable cell factory to produce Spheniscin-2 (Sphe-2/AvBD103b), a Defensin AMP secreted in the stomach of king penguins (<em>Aptenodytes patagonicus</em>). As a proof of concept, <em>P. tricornutum</em> was investigated as a heterologous expression host using the exogenous Defensin ETD151, an optimized form of the antifungal Defensin Heliomicin. This approach enabled optimization of culture conditions and the purification workflow, demonstrating successful recombinant production and recovery of correctly folded ETD151. The optimized gene encoding Sphe-2/AvBD103b, fused to the HASP1 signal peptide to promote extracellular secretion, was introduced into <em>P. tricornutum</em>. Transformants were cultivated and extracellular fractions were analyzed. The presence and integrity of the recombinant Defensin rSphe-2/AvBD103b were confirmed by mass spectrometry, revealing disulfide bridges formation consistent with native folding. Secreted recombinant Defensin rSphe-2/AvBD103b exhibited <em>in vitro</em> antibacterial activity against <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, and <em>Staphylococcus aureus</em>, demonstrating biological functionality. Collectively, these results demonstrate for the first time that <em>P. tricornutum</em> can express and secrete a correctly folded antifungal ETD151 Defensin and an active form of a β-Defensin. These findings establish a promising biotechnological platform for the production and purification of Defensins from the extracellular medium of transformed diatoms.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104792"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic enhancement of biomass and paramylon-enriched material biosynthesis in Euglena gracilis: Physiological responses and gene expression analysis under aromatic amino acid supplementation","authors":"Jiangyu Zhu,&nbsp;Xinyue Guo,&nbsp;Yifei Cai,&nbsp;Xue Xiao,&nbsp;Zhengfei Yang,&nbsp;Yongqi Yin,&nbsp;Minato Wakisaka,&nbsp;Weiming Fang","doi":"10.1016/j.algal.2026.104805","DOIUrl":"10.1016/j.algal.2026.104805","url":null,"abstract":"<div><div>The commercial viability of microalgal bioprocesses is strictly limited by the metabolic trade-off between biomass accumulation and storage polysaccharide synthesis. This study presents a potential metabolic supplementation approach using aromatic amino acids (AAAs) to address this constraint in <em>Euglena gracilis</em>. Supplementation with 0.5 g·L⁻¹ AAAs, particularly phenylalanine, acted as a potent nutritional supplement and potential regulator, increasing biomass by 25.2% while simultaneously boosting the yield of paramylon-enriched material by 2.22-fold. Mechanistic investigation suggested that AAAs may optimize the photosynthetic apparatus by expanding the light-harvesting antenna size and maintaining robust electron transport (ET₀/RC). Furthermore, AAAs mitigated oxidative stress by upregulating superoxide dismutase, thereby converting excess photosynthetic energy into biomass rather than cellular damage. Crucially, RT-qPCR-based gene expression analysis revealed that AAAs upregulated paramylon synthase genes (<em>EgGSL1</em> and <em>EgGSL2</em>) by up to 3.91-fold, which is consistent with enhanced biosynthetic capacity. By facilitating simultaneous cell growth and product accumulation, this benign strategy may bypass the energy- and time-intensive two-stage cultivation typically required for microalgal stress induction. Ultimately, this study provides an integrated “photosynthetic optimization–carbon repartitioning” approach, offering a promising baseline approach for microalgal cultivation and bioproduct accumulation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104805"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic insights into the successional transition from dinoflagellate to cyanobacterial blooms: Dual roles of phytohormones and allelochemicals","authors":"Guimei Wu,&nbsp;Mengxuan Yang,&nbsp;Ji Chen,&nbsp;Jun Zhou,&nbsp;Jie Zhang,&nbsp;Xin Wang,&nbsp;Yanzhao Li,&nbsp;Hongxia Wang,&nbsp;Mingyang Ma,&nbsp;Xinglong Jiang,&nbsp;Mingcan Wu","doi":"10.1016/j.algal.2026.104791","DOIUrl":"10.1016/j.algal.2026.104791","url":null,"abstract":"<div><div>Understanding the succession patterns among harmful microalgae is crucial for managing freshwater ecosystems. The succession from dinoflagellate (<em>Peridinium umbonatum</em>, Pu) to cyanobacterial (<em>Microcystis aeruginosa</em>, Ma) blooms is a common phenomenon，which is often mainly attributed to complex allelopathic interactions. However, the specific chemical compounds driving these interactions and their precise functional roles remain largely unknown. To address this gap, we employed non-targeted UHPLC-MS metabolomics with targeted absolute quantification (LC-QQQ-MS/MS) and functional validation assays to investigate the potential chemical mediators of this interaction. Our results demonstrated a paradoxical, bidirectional relationship: Ma competitively excluded Pu, while Pu's exudates promoted Ma's growth. Through rigorous structural validation with authentic standards (MSI Level 1), we identified key candidate molecules that may potentially mediating this dynamic. Exogenous validation assays suggested that the promotion of Ma is potentially linked to the phytohormone Kinetin 9-riboside and the central metabolite alpha-Ketoglutaric acid secreted by Pu. Conversely, Ma's dominance was likely facilitated by its release of inhibitory allelochemicals, specifically 3-Hydroxykynurenine and a homoisoflavonoid derivative (a Methylophiopogonanone A-like compound). This study moves beyond describing general allelopathy to proposing a potential chemical mediated dual-mechanism for complex microalgal interactions. These findings suggest a sophisticated chemical strategy for competitive dominance and highlight the hypothesized role of phytohormones in mediating phytoplankton community dynamics, offering new insights for predicting and managing harmful algal blooms.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"97 ","pages":"Article 104791"},"PeriodicalIF":5.0,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148554556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}