Xiwei Dai , Xin Chen , Ziqian Cheng , Peizhong Wang , Lianjun Sun , Junbo Huang , Xuezhi Zhang , Haiyang Zhang
{"title":"A multi-dimensional comparative study on the performance of algae removal using various flotation","authors":"Xiwei Dai , Xin Chen , Ziqian Cheng , Peizhong Wang , Lianjun Sun , Junbo Huang , Xuezhi Zhang , Haiyang Zhang","doi":"10.1016/j.algal.2024.103793","DOIUrl":"10.1016/j.algal.2024.103793","url":null,"abstract":"<div><div>Flotation is considered the most cost-effective and efficient algae-water separation technology. However, there are various flotation techniques for algae removal, such as coagulation-flotation (CF), foam flotation (FF), and positively charged bubble flotation (PF). It remains unclear which method is most suitable for removing algae from water bodies and under what specific conditions each technique is most effective. This study systematically compares CF, FF, and PF in terms of algal cell removal efficiency, concentration ratio, flotation kinetics, impact on algal cells, removal efficiency of algal organic matter (AOM), microcystins (MC-LR) and disinfection by-products (DBPs), as well as economic cost analysis. CF is better suited for algae removal in water bodies, including drinking water sources, using fixed installations on shore due to its high removal efficiency, high concentration ratio, low chemical dosage, minimal residuals, and low risk of generating DBPs. FF is more appropriate for non-drinking water sources as it can remove algae and further control algal growth; however, its residual CTAB may pose a threat to drinking water safety. PF is most suitable for in situ algae removal within water bodies, primarily because it does not require stirring or coagulation. Instead, modified bubbles can be directly introduced into the algal distribution layer, where they adhere to algal cells, facilitating algae-water separation. All three flotation methods are economically feasible for algae removal. For FF, the costs of chemicals and electricity are nearly equal, while for CF and PF, the primary cost is electricity. This study provides data to support the selection of appropriate flotation technologies for emergency removal of algal blooms in water bodies.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103793"},"PeriodicalIF":4.6,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microalgal-bacterial co-cultivation on novel bio-coated supports: Evaluation of growth performance in submerged and permeated biofilm cultivation system with cost-benefit assessment","authors":"C.Y. Tong , Chai Ying Kee , Kohsuke Honda , C.J.C. Derek","doi":"10.1016/j.algal.2024.103792","DOIUrl":"10.1016/j.algal.2024.103792","url":null,"abstract":"<div><div>In microalgae mass production, co-cultivation with bacteria and biofilm immobilization hold promise, yet challenges persist in biofilm-based cultivation due to weak cohesion under stress. Hence, a novel bio-coating derived from spent medium and cells (extra-/intra-cellular organic matter from <em>Cylindrotheca fusiformis</em> and <em>Escherichia coli</em>) was applied to microporous membrane in submerged and permeated biofilm systems. Results showed a minimum 25 % improvement in biomass productivity (up to 45 g m<sup>−2</sup>) on bio-coated membranes in permeated system. Mucopolysaccharides in bio-coating facilitated biofilm development and encouraged a 10-fold higher AOM yield (defense mechanism against shearing force) in submerged systems, but biomass productivity was 10 times lower than permeated system. In permeated system, cells on IOM-coated membranes exhibited the highest biomass growth and lipid yield, potentially addressing the biomass-lipid trade-off. Permeated system with low operating cost around 69 $ kg<sup>−1</sup> was a viable cultivation approach, presenting an opportunity to optimize microalgae production facilities.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103792"},"PeriodicalIF":4.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yali Wang , Wenjie Tian , Zhongjie Wang , Xiaobin Wen , Yeguang Li , Yi Ding
{"title":"Identification and characterization of a novel Haematococcus pluvialis strain resistant to Paraphysoderma sedebokerense infection","authors":"Yali Wang , Wenjie Tian , Zhongjie Wang , Xiaobin Wen , Yeguang Li , Yi Ding","doi":"10.1016/j.algal.2024.103791","DOIUrl":"10.1016/j.algal.2024.103791","url":null,"abstract":"<div><div>The commercial culture of <em>Haematococcus pluvialis</em> is threatened by the pathogen <em>Paraphysoderma sedebokerense</em>, leading to substantial losses in the natural astaxanthin industry. This study successfully identified a novel <em>H. pluvialis</em> strain WBG-26, which exhibited high resistance to <em>P. sedebokerense</em> in both laboratory and open raceway ponds. Comparative analysis showed that the resistant strain WBG-26 had a thicker cell wall and lower levels of specific monosaccharides which may be closely related to resistance. RNA-sequencing analysis revealed differential gene expression profiles, with 29 up-regulated and 25 down-regulated genes identified as crucial for resistance. Using bioinformatics analysis, we identified several potential resistance genes and resistance-related genes, such as those encoding receptor-like kinases, leucine-rich repeat, transcription factors, CAZymes, and heat shock proteins, which may play critical roles in defense response. This study represents a comprehensive investigation of multiple defense mechanisms of microalgae, providing insights into breeding disease-resistant microalgal strains for biotechnological applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103791"},"PeriodicalIF":4.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mert Kolukısaoğlu , Ece Polat , Nurgül Çelik Balcı , Mahmut Altınbaş
{"title":"Carbonic anhydrase activity and metabolite variation of different microalgae species at alkaline pHs","authors":"Mert Kolukısaoğlu , Ece Polat , Nurgül Çelik Balcı , Mahmut Altınbaş","doi":"10.1016/j.algal.2024.103778","DOIUrl":"10.1016/j.algal.2024.103778","url":null,"abstract":"<div><div>A significant increase in atmospheric greenhouse gases over the last century has led to the development of several methods and technologies to remove carbon dioxide (CO<sub>2</sub>). Microorganisms produce carbonate minerals through the natural mineralization of CO<sub>2</sub>; however, the feasibility of this process remains in research. This work aimed to study the cultivation of different microalgae under alkaline pH to maintain their potential for carbon mitigation. According to the results, the highest carbonic anhydrase activity has been reached (4.64 mg/g) for <em>Chlamydomonas reinhardtii</em> at a pH of 10. <em>C. reinhardtii</em>, at a pH of 9.5, yielded the highest chlorophyll content (23.58 mg/g), while Spirulina at a pH of 8.5 produced the highest biomass (882.9 mg/L). Also, a positive correlation existed between pH and lipid content for <em>C. reinhardtii</em>. Spirulina, however, exhibits the opposite effect. According to a principal component analysis, there is an opposite relationship between pH and carbonic anhydrase (CA) activity for <em>C. reinhardtii</em> and filamentous-type cyanobacteria from Salda Lake. The following order of the suitability of the microalgae species for high carbon capture is determined by the Analytic hierarchy process method: Spirulina> <em>C. reinhardtii</em> > <em>Chlorella vulgaris</em> > Coccus-type cyanobacteria > Filamentous-type cyanobacteria from Salda Lake. Additionally, this study provided important results regarding the cyanobacteria species isolated from an alkaline lake, Lake Salda. This would contribute to future studies of carbon capture and carbon mitigation mechanisms.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103778"},"PeriodicalIF":4.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biogenic silver nanoparticles synthesized by microalgae: A comprehensive review of eco-friendly wound healing therapies","authors":"Ming-Li Teoh , Li-Lin Lein , Hazel Jing-Yi Leong , Peter Convey","doi":"10.1016/j.algal.2024.103782","DOIUrl":"10.1016/j.algal.2024.103782","url":null,"abstract":"<div><div>Wounds are generally caused by an injury to the skin such as a cut or a puncture. They can be considered in two groups, acute and chronic wounds. Poor wound healing can lead to multiple complications including chronic pain, irritation, unpleasant odours and infections. It is important, therefore, to assist wound healing using suitable treatments as rapidly as possible in order to reduce these complications and improve positive outcomes. Some currently available therapies used in wound care, such as gauzes, steroidal drugs, hyperbaric oxygen therapy and bioengineered cell constructions can lead to negative side effects, limiting their utilization in wound care. With recent advances, nanotechnology has been integrated into wound healing agents, providing an alternative therapeutic approach for the treatment of skin injuries. Amongst the available metal-containing nanoparticles, silver nanoparticles (AgNPs) currently have the greatest potential to be used in wound healing applications due to their strong antimicrobial properties. However, conventional methods of nanoparticle synthesis themselves raise health and environmental concerns due to their use of toxic chemicals and production of harmful waste products.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103782"},"PeriodicalIF":4.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lijuan Wang , Xiaoyan Liu , Jialin Liu , Tianxiang Zheng , Zhaofeng Wang , Xia Liu , Guanghong Luo
{"title":"Effects of different drying methods on the nutritional components, microstructure, and metabolomic profile of Spirulina maxima","authors":"Lijuan Wang , Xiaoyan Liu , Jialin Liu , Tianxiang Zheng , Zhaofeng Wang , Xia Liu , Guanghong Luo","doi":"10.1016/j.algal.2024.103788","DOIUrl":"10.1016/j.algal.2024.103788","url":null,"abstract":"<div><div><em>Spirulina maxima</em> (<em>S. maxima</em>) is considered a potential solution to address the issue of human food security. In this study, three drying methods were employed: spray drying (SD), freeze drying (FD), and vacuum drying (VD), to process fresh <em>S. maxima</em> (FS). A comprehensive nutritional evaluation was conducted using principal component analysis and cluster analysis, along with an untargeted metabolomics study. The results indicated that after SD, FD, and VD treatments, the phycocyanin content decreased by 23 %, 9 %, and 80 %, respectively, compared to that of the FS. The polysaccharide content also significantly decreased by 36 %, 33 %, and 67 %, respectively. Comprehensive analysis showed that SD resulted in the least loss of nutritional indicators in <em>S. maxima</em>. Further metabolomics analysis revealed that prolonged exposure to high temperatures can lead to metabolic disruptions in 2-oxocarboxylic acid metabolism, purine metabolism, amino acid biosynthesis, and starch and sucrose metabolism. Additionally, the levels of coproporphyrin I and coproporphyrin III increased which ultimately led to a decrease in chlorophyll and phycocyanin contents. Therefore, VD resulted in the poorest quality of <em>S. maxima</em>. This study provided a theoretical basis for the quality assessment of <em>S. maxima</em> in response to drying.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103788"},"PeriodicalIF":4.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Experimental evaluation of Scenedesmus javanensis, Halochlorella rubescens, and Chlorolobion braunii for lipid-rich biomass production and phycoremediation of dairy wastewater","authors":"Theja Joseph , Joseph George Ray","doi":"10.1016/j.algal.2024.103789","DOIUrl":"10.1016/j.algal.2024.103789","url":null,"abstract":"<div><div>Phycoremediation uses algae as an ecotechnological tool to recycle eutrophic and polluted wastewater from industries. It is vital for fostering a sustainable circular bioeconomy, as the resulting algal biomass can be utilized as a versatile industrial bioresource. This approach is especially pertinent for the dairy industry, which produces substantial amounts of nutrient-rich dairy wastewater (DWW). The effectiveness of phycoremediation of DWW depends on the knowledge of indigenous algae that can thrive in such conditions and rapidly produce lipid-rich or nutraceutically or otherwise valuable biomass. This study assessed the lab-scale phycoremediation of DWW, emphasizing the biomass production potential and quality of three fast-growing green microalgae: <em>Chlorolobion braunii, Scenedesmus javanensis</em>, and <em>Halochlorella rubescens</em>, which are recognized for their high lipid yield potential in growth media. We assessed nutrient and mineral removal efficiency, biomass yield, productivity, biomass quality, and the biodiesel properties of lipids from these algae after ten days of growth in various dilutions and 100 % DWW. <em>C. braunii</em> demonstrated the highest biomass productivity of 149.32 mg L<sup>−1</sup> d<sup>−1</sup> in 100 % DWW. All species significantly reduced biochemical oxygen demand (BOD), chemical oxygen demand (COD), and total dissolved solids (TDS) of DWW, achieving complete removal of nutrients, along with 80–100 % removal of heavy metals after growth in DWW. <em>C. braunii</em> had a lipid productivity of 49.79 mg L<sup>−1</sup> d<sup>−1</sup> in 100 % DWW, and lipids from <em>C. braunii</em> and <em>S. javanensis</em> were suitable for biodiesel production, highlighting their potential for large-scale industrial phycoremediation of DWW while generating biodiesel-grade lipids and other valuable bioproducts.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103789"},"PeriodicalIF":4.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María del Carmen Romero-Cruz , Antonio Leon-Vaz , José María Vega , Javier Vigara
{"title":"Alterations in nitrogen metabolism caused by heavy metals in the acid-tolerant microalga Coccomyxa onubensis","authors":"María del Carmen Romero-Cruz , Antonio Leon-Vaz , José María Vega , Javier Vigara","doi":"10.1016/j.algal.2024.103784","DOIUrl":"10.1016/j.algal.2024.103784","url":null,"abstract":"<div><div>The microalga <em>Coccomyxa onubensis</em> is an extremophile microorganism with a unique ecosystem (Río Tinto, Huelva, Spain) that contains high amounts of contaminants, including heavy metals, sulphates, and nitrates, in acidic environments (pH 2.5). The current work presents an evaluation of the capacity of <em>Coccomyxa onubensis</em> to assimilate different nitrogen sources under Cu<sup>2+</sup>, Cd<sup>2+</sup>, AsO<sub>3</sub><sup>3−</sup>, AsO<sub>4</sub><sup>3−</sup> and Hg<sup>2+</sup> stress, and the metabolic implications of these stressors. The results showed that ammonium consumption was less affected than nitrate consumption when microalgae were cultivated with heavy metals (except cadmium). The activities of enzymes involved in nitrogen metabolism, such as nitrite reductase (NiR; EC:1.7.7.1), glutamine synthetase (GS; EC:6.3.2.1) and glutamate dehydrogenase (GDH; EC:1.4.1.2) were characterised to determine the Michaelis-Menten constant (<em>K</em><sub><em>m</em></sub>) and optimal temperature and pH values, being 45, 40 and 60 °C and pH values of 7.5, 6.0 and 9.0 for NiR, GS, and GDH, respectively. The effects of different heavy metals on these enzymes were assessed at multiple levels, and the results showed that the enzymatic activity of NiR was downregulated, specially under copper stress, maintaining 23 % of control NiR activity at 2 mM Cu<sup>2+</sup>. The enzymatic activity of GS was upregulated at low concentrations under cadmium and mercury stress (115–120 % of control cultures GS activity at 25 μM Cd<sup>2+</sup> and 50 nM Hg<sup>2+</sup>, respectively) and downregulated at high concentrations of these elements. GDH activity was upregulated in the presence of Cu<sup>2+</sup>, Cd<sup>2+</sup>, and Hg<sup>2+</sup>, with increases up to 192, 155 and 154 % at 1 mM Cu<sup>2+</sup>, 300 μM Cd<sup>2+</sup>, and 250 nM Hg<sup>2+</sup>, respectively. These results provide a better explanation of the effects of heavy metal stress on N metabolism in <em>Coccomyxa onubensis</em>, which may be used as a model eukaryotic organism of the Tinto River acidophilic ecosystem<em>.</em></div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103784"},"PeriodicalIF":4.6,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xianglan Kong , Wei Cao , Yongli Zhang , Yu Yang , Yong Ran
{"title":"Chemical structure and hydrocarbon generation potentials of cyanobacteria Schizothrix calcicole and its resistant biopolymer","authors":"Xianglan Kong , Wei Cao , Yongli Zhang , Yu Yang , Yong Ran","doi":"10.1016/j.algal.2024.103786","DOIUrl":"10.1016/j.algal.2024.103786","url":null,"abstract":"<div><div>Microalgae have attracted much attention because of their great potential in the development of sustainable biofuel. In this study, cyanobacteria <em>Schizothrix calcicole</em> was fractionated into different fractions and characterized by elemental analyses, Rock-Eval pyrolysis, and <sup>13</sup>C NMR. Closed pyrolysis experiments were carried out on the bulk (BL) sample of <em>S. calcicole</em> and its nonhydrolyzable organic matter (NHOM) fraction. The results suggested the NHOM fraction was composed of a saturated and unbranched or weakly branched hydrocarbon chain with a chain length up to 32, which was highly aliphatic resistant biopolymer similar to algaenan in structure, and exhibited higher oil yield (58.1 %) and oil and gas production potentials (OGPs, 63.9 %) than the BL sample did. Moreover, the <em>n-</em>alkanes for the NHOM fraction showed bimodal distribution and were dominated by long chains higher than C<sub>15</sub>. On the contrary, the BL sample exhibited unimodal distribution of <em>n</em>-alkanes, in which middle- and short-chain <em>n</em>-alkanes with chain length <17 were more abundant. In addition, the results indicated <sup>13</sup>C NMR is an effective approach to evaluate hydrocarbon generation potentials. Our investigation identifies aliphatic biopolymers in cyanobacteria <em>S. calcicole</em> and improves the understanding of hydrocarbon generation of its different fractions.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103786"},"PeriodicalIF":4.6,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Loretto Contreras-Porcia , María Dolores Torres , Herminia Domínguez , Julie Queffelec , Florentina Piña , Sheyma Inoubli , Rebeca Aldunate , Francisca C. Bronfman , Nancy Pizarro , Fernanda Toro-Mellado , Maximillian Pozo-Sotelo , María Gallardo-Campos , Benjamín Pinilla-Rojas , Jorge Rivas , Jean Pierre Remonsellez
{"title":"Sustainable biorefinery of Ulva stenophylloides, a green-tide species from the South Pacific coast: A study on antioxidant, oligosaccharide, and ulvan extraction","authors":"Loretto Contreras-Porcia , María Dolores Torres , Herminia Domínguez , Julie Queffelec , Florentina Piña , Sheyma Inoubli , Rebeca Aldunate , Francisca C. Bronfman , Nancy Pizarro , Fernanda Toro-Mellado , Maximillian Pozo-Sotelo , María Gallardo-Campos , Benjamín Pinilla-Rojas , Jorge Rivas , Jean Pierre Remonsellez","doi":"10.1016/j.algal.2024.103785","DOIUrl":"10.1016/j.algal.2024.103785","url":null,"abstract":"<div><div>In this study, biomass characterization and sustainable biorefinery were determined using microwave-assisted extraction to recover valuable biocompounds from the green-tide species <em>Ulva stenophylloides</em>. The biomass exhibits concentrations of 21 % for proteins; 24.6 % for sulfate; 2.1 % for lipids; 27 % for carbohydrates; 15 % and 11 % for chlorophylls <em>a</em> and <em>b</em>, respectively; and 4.6 % for carotenoids. The temperature of the microwave treatment influenced both the yield and quality of biocompounds, with higher concentrations of phenolic compounds and antioxidant activities observed in the soluble extracts at temperatures between 180 °C and 220 °C, and higher concentrations of oligosaccharides obtained at temperatures from 120 °C to 200 °C. Microwave treatment at 120 °C yielded the highest content of ulvans (21 %), as well as favorable molecular weight and viscoelastic properties. Furthermore, the ulvan extract demonstrated no toxic effect in both <em>in vitro</em> and <em>in vivo</em> toxicological assays. These findings highlight the potential of green tides as a renewable biomass source for various biocompounds, opening up possibilities for future biotechnology research.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103785"},"PeriodicalIF":4.6,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}