Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Growth characteristics and nutritional quality of two typical microalgae: Interactive effects of temperature, light, and nitrate","authors":"Yuwei Zhou ,&nbsp;Lele Jiang ,&nbsp;Yuxi Weng ,&nbsp;Haiyang Zong ,&nbsp;Zhenzhen Li ,&nbsp;Juntian Xu ,&nbsp;Futian Li","doi":"10.1016/j.algal.2025.104004","DOIUrl":"10.1016/j.algal.2025.104004","url":null,"abstract":"<div><div>In the context of increasing demand for food products, microalgae garnered considerable interest due to their essential roles in supporting aquatic food webs and serving as bait in aquaculture. In this regard, it is crucial to clarify their optimal culturing conditions, including both single-factor and multiple-factor combinations. In this study, <em>Thalassiosira weissflogii</em> and <em>Pyramimonas</em> sp. were cultivated under five levels of temperature, light intensity, and nitrate to determine their optimum growth conditions. A full factorial experiment was then conducted, employing two levels for each factor (10 and 25 °C for temperature; 50 and 150 μmol photons m⁻² s⁻¹ for light intensity; 2 and 100 μmol L⁻¹ for nitrate concentration) to investigate the growth, photosynthesis, and amino acid and fatty acid compositions of the two species. <em>T. weissflogii</em> exhibited an optimal growth temperature 4 °C higher than that of <em>Pyramimonas</em> sp., while the latter performed better at low temperatures. The full factorial experiment revealed that temperature was the most significant factor influencing the growth of two species. The fatty acid composition of <em>T. weissflogii</em> was primarily affected by temperature changes, with an average 4 % higher percentage of polyunsaturated fatty acids observed at high temperature. Nitrate concentration emerged as the key factor influencing photosynthetic rates, as well as soluble protein and carbohydrate contents, while it was the only factor affecting amino acid profiles. Generally, nitrate limitation resulted in lower photosynthetic rates, higher soluble carbonate contents, while soluble protein content and amino acid composition varied depending on the species and culturing conditions. These findings provide valuable information and reference points for enhancing biomass accumulation and the production of active substances in microalgae cultivation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104004"},"PeriodicalIF":4.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects during co-pyrolysis of microalgal residues and municipal sewage sludge in an H2-containing atmosphere: Pyrolysis characteristic and kinetics","authors":"Ke Song ,&nbsp;Runxi Yin ,&nbsp;Bolun Hao ,&nbsp;Yao Gao ,&nbsp;Zhongdong Zhao ,&nbsp;Anqing Zheng ,&nbsp;Guozhang Chang ,&nbsp;Jie Li ,&nbsp;Jian Zhang","doi":"10.1016/j.algal.2025.104013","DOIUrl":"10.1016/j.algal.2025.104013","url":null,"abstract":"<div><div>In this study, the co-pyrolysis kinetics of municipal sewage sludge (MSS) and <em>Chlorella vulgaris</em> (MCV) in hydrogen atmosphere was studied by thermogravimetric analyzer. Three dynamic models (Friedman, Flynn-Wall-Ozawa and Kissinger-Akahira-Sunose) and DAEM (a model fitting method) were used to accurately fit the experimental data at three heating rates (10, 30 and 50 °C/min). Their physical and chemical properties indicate that they have potential for energy recycling and fuel production. The kinetic model parameters are fitted successfully at different heating rates, and the linear fitting curve of DAEM is within the expectation. The kinetic curve fitting results show that the activation energy changes with the conversion rate, which is directly related to the complexity of molecular chain breaks during pyrolysis. When the proportion of MCV is 20 % (MSS80MCV20), the average activation energy of pyrolysis at 50 % conversion is the lowest, no more than 100 KJ/mol. This data is significantly lower than the average activation energies of the other three samples, which are 179 KJ/mol, 325 KJ/mol and 260 KJ/mol. This study confirmed that the mixed pyrolysis of MSS and MCV in hydrogen atmosphere can significantly change the activation energy <em>Eα</em> of the reaction, which can provide basic data support for the reaction operation conditions of the pyrolysis unit.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104013"},"PeriodicalIF":4.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary assessment of the antioxidant, anti-inflammatory, antidiabetic, and anti-carcinogenic attributes of Australian red seaweeds","authors":"Faezeh Ebrahimi ,&nbsp;Vigasini Subbiah ,&nbsp;Osman Tuncay Agar ,&nbsp;Alistair R. Legione ,&nbsp;Farhad Ahmadi ,&nbsp;Hafiz A.R. Suleria","doi":"10.1016/j.algal.2025.104005","DOIUrl":"10.1016/j.algal.2025.104005","url":null,"abstract":"<div><div>Red seaweeds contain an assortment of valuable phytochemical; however, while their pharmaceutical and therapeutic potential has been previously recognized, many aspects of their bioactive properties remain underexplored, especially among the Australian species. An ultrasonic-assisted extraction method was used to obtain polyphenol-rich extracts from thirteen Australian red seaweeds. Antioxidant activity and anti-inflammatory potential, assessed through assays such as urease inhibition and nitric oxide scavenging, as well as anti-diabetic capacity through α-amylase and α-glucosidase inhibition, were evaluated. <em>Rhodophyllis</em> sp. demonstrated the greatest antioxidant capacity with relative antioxidant capacity index score of 1.43, while <em>Crouania attenuata</em> exhibited superior antidiabetic effects through α-glucosidase and α-amylase inhibition. Notable anti-inflammatory potential was observed in <em>Plocamium</em> and <em>Asparagopsis</em> samples, with <em>A. armata</em> excelling in protease inhibition. Moreover, LC-ESI-QTOF-MS/MS analysis of the extracts tentatively identified 26 polyphenols, indicating the rich bioactive profile of these marine resources. The <em>in vitro</em> assays and phenolic composition highlight the considerable biological potential of Australian Rhodophyta, emphasizing the need for further exploration of these red seaweeds as promising candidates for therapeutic and pharmaceutical applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104005"},"PeriodicalIF":4.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High yield isolation of lutein from high water content Chlorella Vulgaris: A water-plasma and three-phase partitioning approach","authors":"Siying Hu ,&nbsp;Jingcheng Wu ,&nbsp;Yang Liu ,&nbsp;Yangjie Yi ,&nbsp;Md Asraful Alam","doi":"10.1016/j.algal.2025.104008","DOIUrl":"10.1016/j.algal.2025.104008","url":null,"abstract":"<div><div>Extracting lutein from microalgae is a promising approach for lutein production due to the unique advantages of microalgae than terrestrial plants, including superior photosynthetic efficiency, rapid growth rate and potential for carbon mitigation. However, conventional extraction methods from microalgae usually include the utilization of traditional solvents or harsh reaction conditions, resulting in environmental impact and high energy consumption. To address these challenges, an innovative strategy integrating water-plasma with three-phase partitioning (TPP) methods has been proposed for extracting lutein from high water content microalgae. This approach utilizes the reactive species generated by water-plasma, which interact effectively with algal cellular components, while TTP further improved extraction efficiency. Under optimized conditions of 12.9 % DKP concentration, 176.9 W treatment power and 8.8 min treatment time of this method achieved a maximum lutein extraction yield of 0.287 mg/g microalgae. Importantly, this lutein production process eliminates the need for the drying of microalgae biomass, thereby significantly diminishing energy consumption and enhancing the economical scalability of lutein production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104008"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgal mixotrophic cultivation enhances lignocellulosic pretreatment wastewater utilization: Inhibitors detoxification process and C5/C6 sugar high-value conversion","authors":"Huiying Wang ,&nbsp;Lei Qin ,&nbsp;Wei Qi ,&nbsp;Mostafa Elshobary ,&nbsp;Wen Wang ,&nbsp;Xiang Wang ,&nbsp;Zhongming Wang ,&nbsp;Shunni Zhu","doi":"10.1016/j.algal.2025.104007","DOIUrl":"10.1016/j.algal.2025.104007","url":null,"abstract":"<div><div>Algae, with their strong carbon fixation capacity and ability to accumulate high-value products, hold significant potential for utilizing lignocellulosic pretreatment wastewater (LPW). However, effective bio-detoxification of inhibitors and the efficient utilization of C5/C6 sugars in LPW are critical for the successful application of algae in resource utilization. This study investigates the effects of typical LPW inhibitors—furfural and vanillin—on the physiological and biochemical properties of <em>Chlorella sorokiniana</em> under mixotrophic conditions. Additionally, the biodetoxification of these inhibitors and the conversion of C5/C6 sugars into high-value products were examined. The results demonstrated that <em>C. sorokiniana</em> could tolerate up to 0.5 g/L furfural and 0.25 g/L vanillin during mixotrophic growth. However, exposure to furfural, vanillin, and their combination significantly inhibited algal antioxidant enzyme activities and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) function, leading to damage to the photosynthetic apparatus and a reduction in the conversion of high-value products. Despite these challenges, the algae were able to convert these inhibitors into less toxic compounds, such as alcohols and acids, through both photosynthesis and respiration, achieving a 100 % conversion rate within the first three days of exposure. Moreover, <em>C. sorokiniana</em> effectively utilized C6 sugars for biomass accumulation, which comprised 41.12 %–55.96 % protein, 12.32 %–19.89 % lipids, and 20.06 %–44.65 % carbohydrates. Additionally, the algae successfully converted C5 sugars into xylitol, a high-value product. These findings provide valuable insights into the potential of algal systems for resource utilization from lignocellulosic biomass, as well as the cost-effective production of valuable algal biomass and by-products.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104007"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and transcriptomic responses of soil green alga Desmochloris sp. FACHB-3271 to salt stress","authors":"Tianli Li ,&nbsp;Yanwen Zhang ,&nbsp;Yunlu Jia ,&nbsp;Zichao Gong ,&nbsp;Xiang Fan ,&nbsp;Qi Zhang ,&nbsp;Lingling Zheng ,&nbsp;Jin Liu ,&nbsp;Dongyi Wang ,&nbsp;Fan Ye ,&nbsp;Fang Bai ,&nbsp;Lirong Song","doi":"10.1016/j.algal.2025.104006","DOIUrl":"10.1016/j.algal.2025.104006","url":null,"abstract":"<div><div>Salinity, a rising global threat to agriculture exacerbated by climate change, may be mitigated through salt-soil bioremediation using microalgae. Most studies on stress responses have focused on aquatic algae, leaving soil microalgae largely unexplored. This study investigates the short- and long-term salt stress responses in the salt-tolerant soil microalga <em>Desmochloris</em> sp. FACHB-3271, combining phylogenetic, physiological, and biochemical analyses. Under salt stress, <em>Desmochloris</em> sp. showed sustained growth, with elevated photosynthetic activity and increased polysaccharide, soluble proteins, and total lipid contents. Linoleic acid was the predominant fatty acid, comprising 48.23 % of the total lipids. Long-term salt stress enhanced energy metabolism (chlorophyll synthesis, glycolysis) and osmotic regulation (fatty acid synthesis and degradation, starch and sucrose synthesis), while short-term stress induced dynamic changes. Three signaling pathways (cAMP, MAPK, and ABC transporters) were identified as regulators of salt stress changes. Notably, genes related to vitamin B12 for synthesis exhibited significant changes. These findings provide insights into the salt stress mechanisms of soil microalgae, offering genetic resources for saline-alkali soil remediation, for genetic modification of microalgae for biofuel production and novel approaches to gene discovery in non-genome-referenced algae.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104006"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transformation of seaweed waste into valuable phycocolloids able of reducing the arsenic, mercury and vanadium toxicity in aqueous solutions","authors":"Angela Macrì ,&nbsp;Eleonora Agostino ,&nbsp;Damiano Spagnuolo ,&nbsp;Domenico Trombetta ,&nbsp;Antonella Smeriglio ,&nbsp;Antonio Spanò ,&nbsp;Marina Morabito ,&nbsp;Giuseppa Genovese ,&nbsp;Maria Teresa Caccamo ,&nbsp;Salvatore Magazù ,&nbsp;Marco Sebastiano Nicolò ,&nbsp;Vincenzo Zammuto ,&nbsp;Concetta Gugliandolo","doi":"10.1016/j.algal.2025.104002","DOIUrl":"10.1016/j.algal.2025.104002","url":null,"abstract":"<div><div>Heavy metal pollution is a major environmental and human concern. The conversion of invasive macroalgae biomass, usually treated as waste, into high-valuable and eco-friendly products, is gaining growing interest in removing heavy metals (HMs). In this study, phycocolloids (PCs) extracted by a microwave-assisted method from <em>Agardhiella subulata</em> (κ-carrageenan), <em>Sargassum muticum</em> (alginate) and <em>Ulva ohnoi</em> (ulvan) were preliminary evaluated for their ability to reduce the toxicity of arsenite (As(III)), mercury (Hg(II)) and vanadate (V(V)), using inhibition assays of bioluminescence and viability of the marine diatom <em>Phaeodactylum tricornutum</em>. Although all PCs (1000 μg mL⁻¹) slightly reduced As(III) toxicity (≤10 %), all strongly reduced V(V) toxicity (≥70 %), only ulvan was also able to reduce more than twofold the Hg(II) toxicity. To investigate the mechanisms involved in the toxicity reduction, PCs were evaluated for: i) the ability to adsorb As(III), Hg(II) and V(V), and ii) the interaction between PCs functional groups and HMs. PCs adsorbed Hg(II) more efficiently than V(V) or As(III), being ulvan the most efficient in adsorbing Hg(II) (74 %), while alginate and κ-carrageenan were more efficient in adsorbing V(V) (40 %). Interestingly, after reaching the maximum adsorption capacity (30 min), Hg(II) and V(V) remained adsorbed to the PCs for a long time(over 240 min). As resulted by ATR-FTIR analysis, the biosorption mechanisms were mainly attributed to electrostatic interactions between Hg(II) and the functional groups (–COOH and C<img>O) of ulvan, whereas specific binding sites of alginate and κ-carrageenan complexed V(V). Although the adsorption of V(V) by ulvan was negligible, the spectra indicated that vanadate was chemically reduced to a lesser toxic form. Our results suggest that these PCs, extracted by an eco-friendly procedure, could be used to develop new strategies to remediate Hg(II) and V(V) pollution and simultaneously counteract their harmful effects in aquatic environments.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104002"},"PeriodicalIF":4.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of using freshwater microalgae to remove triclosan from aqueous media","authors":"Ana Gisell Pazmino-Sosa ,&nbsp;Jean-François Blais ,&nbsp;Pascale Champagne","doi":"10.1016/j.algal.2025.104001","DOIUrl":"10.1016/j.algal.2025.104001","url":null,"abstract":"<div><div>Triclosan, [5-chloro-2-(2,4-dicholophenoxy)phenol] (TCS), a broad-spectrum antimicrobial agent found in many personal care products, has raised concerns due to its presence in the environment. TCS has been associated to harmful effects, including oxidative damage in golfish cells, increased lipid peroxidation in clams, and disruption of the hypothalamic-pituitary-gonadal axis in Catla fish, and its potential contribution to antimicrobial resistance. This study evaluates the feasibility of using two freshwater microalgae species to remove TCS from aqueous media by 1) determining the toxicity of TCS on algal cultures, 2) evaluating their potential to remove TCS, and 3) identifying the TCS degradation kinetics. The toxicity test assessed various concentrations of TCS (0.06, 0.10, 0.20, 0.30, 1 mg L⁻¹) on <em>Chlorella vulgaris</em> and <em>Scenedesmus obliquus</em> growth. Results showed that <em>C. vulgaris</em> was entirely inhibited by concentrations exceeding 0.10 mg L⁻¹. In comparison, <em>S. obliquus</em> tolerated up to 0.30 mg L⁻¹ after six days of lag phase, but 1 mg L⁻¹ was toxic for both species. The removal efficiency achieved by <em>S. obliquus</em> was between 79 % and 94 % across all concentrations tested, while <em>C. vulgaris</em> achieved 70–95 % removal only in concentrations lower than 0.10 mg L⁻¹. The degradation kinetics revealed that the TCS half-life in wastewater was 1.3 days when <em>S. obliquus</em> was present, highlighting its potential to enhance pollutant removal. This study provides insights into the use of <em>S. obliquus</em> for removing contaminants from natural environments, contributing to understanding TCS dynamics in ecosystems with the presence of microalgae.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104001"},"PeriodicalIF":4.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic advantage promotes Microcystis competitiveness against Scenedesmus: Synchronized dynamics and structure shifts in symbiotic microbiomes","authors":"Zeshuang Wang,&nbsp;Zhaowen Hu,&nbsp;Zengling Ma,&nbsp;He Zhang,&nbsp;Peng Xiao,&nbsp;Siyu Yang,&nbsp;Jun Zuo,&nbsp;Renhui Li","doi":"10.1016/j.algal.2025.104000","DOIUrl":"10.1016/j.algal.2025.104000","url":null,"abstract":"<div><div><em>Microcystis</em> threaten aquatic ecosystems because of its low nutritional value and cyanotoxin production. While its stress-induced photosynthetic enhancement under nutrient limitation is recognized, the role of interspecific competition in driving photo-physiological adaptation remains unclear. Field observations reveal synchronized restructuring of <em>Microcystis</em>-associated symbiotic microbiomes with algal dominance shifts, while their dynamics in laboratory competition systems remain poorly understood. We investigated <em>Microcystis aeruginosa</em>–<em>Scenedesmus obliquus</em> interactions under controlled competition, revealing: (1) <em>S. obliquus</em> presence triggered <em>M. aeruginosa</em> photosynthetic upregulation; (2) the specific growth rates and carrying capacities of both algae were mutually inhibited, with <em>M. aeruginosa</em> exerting a stronger inhibitory effect on <em>S. obliquus</em> than vice versa; (3) the structure and composition of the symbiotic microbial community changed in tandem with the increasing dominance of <em>M. aeruginosa</em>; (4) the relative abundance of bacteria associated with <em>M. aeruginosa</em>, including <em>Rhodobacter</em>, <em>Porphyrobacter</em>, and <em>Methylophilus</em>, gradually increased in parallel with the dominance of <em>M. aeruginosa</em>. These results indicate that enhanced photosynthesis facilitates the competitive advantage of <em>M. aeruginosa</em> over <em>S. obliquus</em> and emphasize the synchronized dynamics of the symbiotic microbial community, resembling patterns observed in natural waters. Our findings provide insights into cyanobacterial succession, informing bloom prediction and microbial management strategies.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104000"},"PeriodicalIF":4.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constructing a stable mannitol biosynthetic pathway in cyanobacteria via the introduction of heterologous mannitol dehydrogenase","authors":"Jiahui Sun ,&nbsp;Xiangyi Yuan ,&nbsp;Jinhui Tang ,&nbsp;Huili Sun ,&nbsp;Guodong Luan ,&nbsp;Xuefeng Lu","doi":"10.1016/j.algal.2025.103998","DOIUrl":"10.1016/j.algal.2025.103998","url":null,"abstract":"<div><div>Mannitol, a valuable sugar alcohol, finds extensive application in food and medicine industries. The advancements of synthetic biology technology have unlocked the potential of cyanobacteria for directly converting CO₂ into mannitol. Previously, attempts have been made to engineer mannitol synthesizing cyanobacteria strains by introducing a heterologous pathway consisting of mannitol-1-phosphate dehydrogenase (Mtld) and mannitol-1-phosphatase (M1Pase). However, the recombinant strains generally encountered stability and yield issues. In this work, an alternative mannitol synthesizing pathway in which a heterologous mannitol dehydrogenase (Mdh) that converts fructose into mannitol was engineered and evaluated in <em>Synechococcus elongatus</em> PCC 7942, and photosynthetic cell factories simultaneously producing mannitol and fructose were constructed. A salt stress-induced cultivation method was initially used to achieve stable mannitol synthesis by leveraging the property of mannitol as a compatible solute under salt stress. The engineered strain exhibited an improved mannitol yield of 0.95 g/L, accompanied by the synthesis of 1.05 g/L fructose during a 15-day cultivation process under hypersaline induction. By inductively controlling the upstream sucrose metabolism, salt-independent mannitol-fructose co-production can be realized in long-term cultivation. This led to the accumulation of 0.7 g/L mannitol and 0.27 g/L fructose in the recombinant strain, with no spontaneous mutations observed in the mannitol biosynthesis operon during a 31-day cultivation process. This study represents the first instance of constructing a cyanobacterial mannitol cell factory employing the Mdh-driven pathway. The findings in this work provided new strategies for engineering efficient and stable photosynthetic cell factories of mannitol in future, also sheds light on the plasticity of cyanobacterial sugar metabolism networks.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 103998"},"PeriodicalIF":4.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}