Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Optimizing semi-continuous cultivation of Arthrospira platensis C1 for phycocyanin production using dynamic modeling","authors":"Noppathonthan Aowtrakool ,&nbsp;Teeraphan Laomettachit ,&nbsp;Marasri Ruengjitchatchawalya","doi":"10.1016/j.algal.2025.104157","DOIUrl":"10.1016/j.algal.2025.104157","url":null,"abstract":"<div><div>The production of C-phycocyanin (C-PC) from <em>Arthrospira platensis</em> is strongly influenced by nitrogen availability. In the present study, we used our previously developed ODE-based dynamic model to simulate the effects of ammonium concentrations ranging from 0.25 to 2.0 mM N on C-PC and biomass production in <em>A. platensis</em> C1. Comparisons with experimental data demonstrated the model's high predictive accuracy in both batch and semi-continuous systems, supported by low RMSE values and high correlation coefficients. Model-guided optimization of semi-continuous processes revealed that an initial ammonium concentration of 1.8 mM N, with a 36 h harvesting interval, significantly enhanced the C-PC content to approximately 28 % DW. This model offers a robust framework for managing nitrogen levels and optimizing cultivation intervals to maximize C-PC content. These insights underscore the model’s utility as a tool for optimizing C-PC production while promoting sustainable practices in microalgae cultivation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104157"},"PeriodicalIF":4.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved lipid production in Chlorella sp. UJ-3 using Fe3O4 nanoparticles assisted by an alternating magnetic field and transcriptome analysis","authors":"Feng Wang ,&nbsp;Wenjing Ren ,&nbsp;Wen Guan ,&nbsp;Shuhao Huo ,&nbsp;Bin Zou ,&nbsp;Jingya Qian ,&nbsp;Anzhou Ma ,&nbsp;Guoqiang Zhuang ,&nbsp;Ling Xu","doi":"10.1016/j.algal.2025.104147","DOIUrl":"10.1016/j.algal.2025.104147","url":null,"abstract":"<div><div>In this study, a process intensification strategy combining magnetic Fe₃O₄ nanoparticles (NPs) and an alternating magnetic field (MF) was employed to enhance lipid production in <em>Chlorella</em> sp. UJ-3. Among the tested MFs at various frequencies, the alternating MF at 200 Hz demonstrated the most significant promotion of cell growth and lipid production. Under this treatment, biomass increased by 64.5 %, and total lipid production was enhanced by 170.8 % compared to the untreated control. With the MF and Fe₃O₄ NPs treatment, algal cells regulated their antioxidant defense system, resulting in increased activities of related antioxidant enzymes to counteract the elevated reactive oxygen species induced by the MF and Fe₃O₄ NPs. The regulatory mechanism of Fe₃O₄ NPs on <em>Chlorella</em> sp. metabolism under a MF was analyzed at the transcriptome level. The addition of Fe₃O₄ NPs promoted the synthesis and desaturation of fatty acids while reducing their β-oxidation. The expression of most enzyme genes in the glycolytic pathway were downregulated, while several enzyme genes in the pentose phosphate pathway were upregulated. The expression of superoxide dismutase and catalase genes was also upregulated under Fe₃O₄ NPs treatment. This study provides an efficient method for enhancing lipid production in microalgal cells and offers valuable insights into the biological effects of magnetic NPs on algal metabolism.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104147"},"PeriodicalIF":4.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic impact of elevated CO2 and photo-bioreactor illumination surface area on marine microalgal biomass and bio-chemicals production","authors":"Venkatesan Ajithkumar ,&nbsp;V. Isaimozhi ,&nbsp;Bhavika Mehta ,&nbsp;Kirti Singhal ,&nbsp;Shreya Sadukha ,&nbsp;Koustav Biswas ,&nbsp;Arup Ghosh ,&nbsp;Ramalingam Dineshkumar","doi":"10.1016/j.algal.2025.104156","DOIUrl":"10.1016/j.algal.2025.104156","url":null,"abstract":"<div><div>This study investigates the synergistic effects of enriched CO₂ and illumination surface area of a photo<strong>-</strong>bioreactor (PBR) on the growth, biochemicals and pigment production of marine microalgae. Accordingly, marine <em>Chlorella</em> sp. and <em>D. salina</em> were cultivated under various conditions, including ambient CO₂ (AC: 0.04 %, <em>v</em>/v) and high CO₂ (HC: 15 %, v/v), with two illumination surface area-to-volume (IS/V) ratios: low (0.22 cm⁻¹) and high (0.44 cm⁻¹). The results demonstrated that both marine strains showed significant changes in biochemical yields under different cultivation conditions, with the highest biomass yield of 2.01–2.07 g L⁻¹ observed when HC and high IS/V ratio were combined. These biomass yields are 107–132 % higher compared to AC and low IS/V conditions. <em>Chlorella</em> sp. and <em>D. salina</em> cultured under the combined HC with high IS/V conditions significantly improved the production of lutein and lipid by 163–417 % and 12–36 %, respectively. However, the maximum protein (178–188 mg g⁻¹) and carbohydrate (182–284 mg g⁻¹) yields in both strains were achieved under AC with high IS/V and low IS/V conditions, respectively. CO₂ sequestration of 0.40–0.51 g L⁻¹ d⁻¹ exhibited by these strains are comparatively higher than most of the marine microalgae reported in the literature. The novelty of this study lies in the demonstration of the synergistic effects of elevated CO₂ levels (15 %, <em>v</em>/v), equivalent to coal<strong>-</strong>fired flue gas, and PBR's illumination surface area on marine microalgal cultivation in a seawater-supplemented medium, leading to optimized CO₂ fixation, biomass yield, and bio-chemicals production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104156"},"PeriodicalIF":4.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing productivity and susceptibility attributes towards a rapid management-risk assessment of seaweed","authors":"D. Milla-Figueras ,&nbsp;P. Torres ,&nbsp;A.Z. Botelho ,&nbsp;A. Larrea ,&nbsp;M.I. Parente ,&nbsp;P. Afonso","doi":"10.1016/j.algal.2025.104160","DOIUrl":"10.1016/j.algal.2025.104160","url":null,"abstract":"<div><div>The challenge of balancing conservation with sustainable use of marine resources faced by fisheries managers is quite often constrained by limited data on key species. This has become particularly true in the case of seaweed, which provide essential habitats for a diverse range of species in marine ecosystems and have long been valued by humans as food or, more recently, for their industrial applications. The expanding seaweed industry relies heavily on harvesting but historical declines in seaweed populations raise concerns over sustainability, leveraged by a general data-poor scenario for these species. To address this, we propose adapting the Productivity and Susceptibility Analysis (PSA) method—traditionally used in fishery assessments—for seaweeds. This novel approach incorporates the unique biology of seaweeds, including growth, reproduction, and susceptibility to environmental changes and harvesting practices, to evaluate their vulnerability. We applied this adapted PSA framework to a case study of seaweed harvesting in the Azores Archipelago, mid-north Atlantic, demonstrating its applicability in future science-based seaweed fisheries management srtategies. Among the 18 species studied, we identified five of high vulnerability (<em>Cystoseira pustulata</em>, <em>Fucus spiralis</em>, <em>Gongolaria abies-marina</em>, <em>Laminaria ochroleuca</em>, and <em>Ericaria selaginoides</em>). Our adapted PSA framework also considers local ecological impacts, stakeholder input, and relevant environmental factors, providing targeted management recommendations. While such a PSA does not replace stock assessments, it serves as a valuable risk assessment tool, identifying species needing greater management attention despite data limitations. Ultimately, this framework supports local, national and international policy and management measures, contributing to the conservation of coastal marine ecosystems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104160"},"PeriodicalIF":4.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of microalgae: Efficient conversion of Chlorella sp. biomass into ethyl levulinate","authors":"G.T. Jeong","doi":"10.1016/j.algal.2025.104151","DOIUrl":"10.1016/j.algal.2025.104151","url":null,"abstract":"<div><div>Microalgae have significant promise as sustainable bioresources for the synthesis of biofuels, biochemicals, and biomaterials. This study synthesized ethyl levulinate (EL) by thermochemical conversion of microalgae using methanesulfonic acid (MSA) as a catalyst. To optimize the conversion factors influencing the synthesis of EL from microalgae, the one-factor-at-a-time technique was applied to the reaction performed in ethanol media. The addition of water to the reactant strongly inhibited the formation of EL. Under the optimized conditions of 2.5 % microalgae biomass with 0.5 M MSA at 170 °C for 45 min, a 65.76 ± 0.57 % EL yield carbohydrate content basis (34.31 ± 0.30 % based on biomass amount) was achieved. Furthermore, the EL yields increased linearly with the combined severity (CS) until 3.4 and then slightly declined thereafter. The highest yields were achieved near CS 3.4. The results indicated that microalgae, which are easily accessible and potentially significant bioresources, together with MSA, an ecologically benign and highly effective acid catalyst, might be used for the production of promising biorefinery products.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104151"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An efficient method for producing polysaccharides with high antioxidant activity from Caulerpa lentillifera and evaluating their stability","authors":"Ratana Chaiklahan,&nbsp;Nattayaporn Chirasuwan,&nbsp;Thanyalak Srinorasing,&nbsp;Channarong Suaisom","doi":"10.1016/j.algal.2025.104153","DOIUrl":"10.1016/j.algal.2025.104153","url":null,"abstract":"<div><div>Algal polysaccharides are widely utilized in various industries due to their functional and bioactive properties. However, commercial-scale polysaccharide production requires simple, efficient, and low-cost processes. This study developed a practical and scalable method for producing polysaccharides from <em>Caulerpa lentillifera</em>. Given its high moisture and polysaccharide content, a squeezing method followed by spray drying was evaluated. The squeezed fresh polysaccharide (SFP) exhibited higher levels of bioactive compounds and stronger antioxidant activity than the polysaccharide extracted at 90 °C (90-FP). Similarly, the spray-dried polysaccharide (SDP), obtained from squeezing and spray drying, showed superior bioactive compound content and antioxidant activity compared to the ethanol-precipitated polysaccharide (EPP). The SDP extract contained 51.25 % total sugars, 7.33 % sulfates, 2.62 % uronic acid, 7.99 % protein, and 8.64 mg GAE/g total phenolic content. It exhibited strong antioxidant activity, with 50 % inhibitory concentration (IC₅₀) values of 0.73 mg/mL for 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, 9.57 mg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 26.96 mg/mL for lipid peroxidation (LPO) inhibition, and 0.88 mg/mL for ferrous ion-chelating (FIC) ability. Its ferric-reducing antioxidant power (FRAP) was 7.49 mg Fe²⁺/g extract. Additionally, the SDP extract demonstrated high stability suitable for industrial application, with a shelf life of 8.78 months and a half-life of 57.76 months at room temperature.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104153"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selecting phytohormone treatments through Grey Relational and Principal Component Analysis for microalgal bioactives production","authors":"S. Kichouh-Aiadi,&nbsp;J.J. Gallardo-Rodríguez,&nbsp;M.C. Cerón-García,&nbsp;L. López-Rosales,&nbsp;A. Sánchez-Mirón,&nbsp;F. García-Camacho","doi":"10.1016/j.algal.2025.104152","DOIUrl":"10.1016/j.algal.2025.104152","url":null,"abstract":"<div><div>Microalgae represent a promising sustainable source of high-value compounds including carotenoids and omega-3 fatty acids, which have applications in nutraceuticals, pharmaceuticals, cosmetics, and functional foods. Fucoxanthin, a xanthophyll carotenoid, exhibits anti-obesity, antidiabetic, antioxidant, and anti-inflammatory properties, while omega-3 fatty acids like docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are essential nutrients particularly important in aquaculture and human health. However, commercial production faces challenges including low productivity and high processing costs. This study aimed to evaluate the effects of twelve phytohormones (auxins, cytokinins, gibberellins, abscisic acid, jasmonates, and salicylic acid) on biomass productivity, fucoxanthin content, and fatty acid profiles of three biotechnologically relevant microalgae: <em>Chrysochromulina rotalis</em>, <em>Heterosigma akashiwo</em>, and <em>Entomoneis sp.</em> Phytohormones were tested at concentrations in the range 0.5–30 mg L⁻¹ (100 mg L⁻¹ in the case of ethanolamine). Grey Relational Analysis (GRA) and Principal Component Analysis (PCA) revealed species-specific optimal treatments: salicylic acid (30 mg L⁻¹) for <em>C. rotalis</em> increased fucoxanthin by 43 % and omega-3 fatty acids by 36 %; gibberellic acid (30 mg L⁻¹) for <em>H. akashiwo</em> improved fucoxanthin by 27 % and EPA by 25 %; and 6-benzylaminopurine (15 mg L⁻¹) for <em>Entomoneis sp.</em> enhanced fucoxanthin by 34 % and total fatty acids by 28 %. These treatments demonstrated significant improvements in both biomass productivity and high-value metabolite accumulation with potential cost-effectiveness. The molecular mechanisms underlying these improvements involve complex interactions between hormone signaling pathways, primary metabolism, and secondary metabolite synthesis. Further exploration of wastewater-derived phytohormones could reduce production costs and enhance sustainability in microalgae-based bioprocesses.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104152"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient-driven biomass variation in Ulva prolifera: Experimental insights for predicting Yellow Sea green tide magnitude","authors":"Tingting Wu ,&nbsp;Wei Liu ,&nbsp;Jingfei Liu ,&nbsp;Jingyi Sun ,&nbsp;Hongtao Liu ,&nbsp;Yutao Qin ,&nbsp;Huanhong Ji ,&nbsp;Jianheng Zhang ,&nbsp;Peimin He","doi":"10.1016/j.algal.2025.104143","DOIUrl":"10.1016/j.algal.2025.104143","url":null,"abstract":"<div><div>The green tide in the Yellow Sea, one of the largest globally, is primarily driven by the biomass of <em>Ulva prolifera</em>, which is influenced by seawater nutrient levels, particularly inorganic nitrogen and phosphorus. This study aimed to clarify the relationship between <em>U. prolifera</em> growth and seawater nitrogen and phosphorus concentrations. Experiments were conducted in the northern Jiangsu coastal waters using three nutrient concentration gradients (high, medium, and low) to assess their impact on <em>U. prolifera</em> biomass. Additionally, the proportional relationship between nutrient uptake by algal tissue and nutrient depletion from the water was analyzed. Results showed a significant positive correlation between nitrogen and phosphorus concentrations and <em>U. prolifera</em> growth, with Spearman's correlation coefficients exceeding 0.4 and <em>p-</em>values &lt;0.05. Increased nitrogen and phosphorus levels promoted faster growth and higher biomass, with NH₄⁺ having the most pronounced effect, leading to a maximum weight gain of 107.4 %. Nutrient uptake measurements indicated that for each gram of nitrogen absorbed, <em>U. prolifera</em> biomass increased by 277.9 g, and for each gram of phosphorus absorbed, biomass increased by 4056.5 g. In conclusion, nutrient enrichment accelerates <em>U. prolifera</em> growth, and limiting nitrogen and phosphorus inputs could reduce biomass accumulation in the early stages of green tide formation. These findings provide valuable insights for developing strategies to manage and control green tide events and protect marine ecosystems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104143"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolome profiling and sensory analysis revealed the seafood flavor enhancement in the treated biomass powder of golden Auxenochlorella pyrenoidosa mutant strain by high-pressure homogenization","authors":"Jiayan Xie ,&nbsp;Xiangru Shi ,&nbsp;Dong Wei","doi":"10.1016/j.algal.2025.104150","DOIUrl":"10.1016/j.algal.2025.104150","url":null,"abstract":"<div><div>As a novel resource of algae-based alternatives protein, the golden biomass of <em>Auxenochlorella pyrenoidosa</em> mutant (CX41 strain) was prepared by fermentation in shake flasks, following high-pressure homogenization (HPH) for cell disruption. The preferred parameters were obtained with 50 % (wet <em>w</em>/<em>v</em>, wet weight/volume) of CX41 biomass under 950 bars for HPH treatment in a single pass. Surprisingly, the enhanced pleasant flavor was observed in CX41 powder after HPH treatment (HPH-CX41). Electronic tongue and odor sensory analyses revealed significant increases of 15.22 % for the umami and 27.59 % for shredded squid odor in HPH-CX41 than that of CX41 powder. Profiling of non-volatiles verified significant increase of free amino acids content in HPH-CX41 powder. The amino acids and oligopeptides like glutamic acid and arginine-leucine increased by 4.56 and 16.07 folds respectively, resulting in a significant increase in umami. Analysis of volatiles and their relative odor activities indicated that the dominant odor in both powders was roasted, attributed to furans and pyrazines like 2-methyl-3-furanthiol, 5-methyl-2-furanmethanethiol, and 2, 3-diethyl-5-methyl-pyrazine. Their increased contents in HPH-CX41 powder resulted in an increased roasted intensity. The relative odor activity value (rOAV) of 2-pentyl-furan, with pleasant aroma of squid, significantly increased, leading to the enhanced seafood flavor of HPH-CX41 powder. This is the first report to investigate the metabolite basis of flavor enhancement in <em>Auxenochlorella pyrenoidosa</em> biomass powder by HPH technology, providing a potential application of HPH-CX41 powder as novel high-protein and flavoring agent in microalgae-based seafood substitutes.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104150"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanol-driven mixotrophic cultivation enhances biomass production and CO2 capture in Euglena gracilis for sustainable bioresource utilisation","authors":"Sahutchai Inwongwan ,&nbsp;Kritsana Duangjan ,&nbsp;Phinyarat Sensupa ,&nbsp;Kittiya Phinyo ,&nbsp;Khomsan Ruangrit ,&nbsp;Chayakorn Pumas ,&nbsp;Jeeraporn Pekkoh","doi":"10.1016/j.algal.2025.104148","DOIUrl":"10.1016/j.algal.2025.104148","url":null,"abstract":"<div><div>This study explored the enhancement of biomass production and CO₂ capture in <em>Euglena gracilis</em> through mixotrophic cultivation using ethanol as a high redox potential substrate. Three cultivation strategies were compared: photoautotrophic, heterotrophic, and mixotrophic. The mixotrophic condition yielded the highest biomass production at 1.79 g L⁻¹, markedly exceeding the yields observed in heterotrophic (0.84 g L⁻¹) and photoautotrophic (0.11 g L⁻¹) cultures. Although ethanol consumption was comparable between mixotrophic and heterotrophic conditions, the presence of light in the mixotrophic system appeared to enhance cellular metabolism, presumably by improving cellular energy availability. This led to greater biomass production and CO₂ fixation. Correspondingly, the mixotrophic culture achieved a CO₂ capture rate of 0.07 g CO₂ L⁻¹ day⁻¹, representing a 600 % increase compared to the 0.01 g CO₂ L⁻¹ day⁻¹ observed under photoautotrophic conditions, and exhibited a higher total carbon content in the biomass (41 % versus 28.9 %). These findings provide a proof-of-concept for using ethanol-supplemented mixotrophic cultivation to significantly boost both biomass production and CO₂ capture in <em>E. gracilis</em>, underscoring its potential for applications in carbon capture and sustainable biomass production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104148"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}