Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Digestate dilution shapes carbohydrate and pigment production during microalgal and cyanobacterial-based biogas upgrading","authors":"Matilde Ciani ,&nbsp;Laura Vargas-Estrada ,&nbsp;Alessandra Adessi ,&nbsp;Raúl Muñoz","doi":"10.1016/j.algal.2025.104290","DOIUrl":"10.1016/j.algal.2025.104290","url":null,"abstract":"<div><div>Microalgae and cyanobacteria offer a promising platform for integrating sustainable technologies aligned with circular and green economy goals. However, current studies often focus on a limited number of genera and overlook how centrate dilution influences metabolite production. This study investigates the potential of the freshwater microalga <em>Parachlorella hussii</em> N9 and the marine cyanobacterium <em>Cyanothece</em> sp. CE4 for photobiological biogas upgrading coupled with nutrient recovery from centrate, assessing the impact of centrate dilution on carbohydrate and pigment content. By varying centrate concentration (5–50 %) in tap or seawater, this research explores how the biogas-to-centrate ratio can be adjusted for biomass production, TN and CO₂ abatement, and to target specific metabolites, advancing circular bioeconomy strategies. The microalga exhibited faster growth than the cyanobacterium, achieving the stationary phase in three days, and higher cellular and soluble carbohydrate productivity (up to 237 and 75 mg L⁻¹d⁻¹, respectively). CO₂ abatement (almost complete in all treatments) reached ∼513 ± 28 mg L⁻¹ of culture, while nitrogen removal considering initial centrate concentration ranged between 32 and 250 mg N L⁻¹, but 100 % TN removal was exhibited only with the lower centrate concentrations (5–10 %). These lower concentrations also induced the highest carbohydrate content in biomass (41–44 % dw). In contrast, pigment content increased with higher centrate concentrations: the microalga reached 3.6 % dw of chlorophyll <em>a</em>t 50 % centrate, while the cyanobacterium produced up to 0.6 % dw of C-phycocyanin; both strains showed similar carotenoid content (0.4–0.5 % dw). This study highlights the potential of adjusting centrate dilution to target microalgal metabolism for integrated CO₂ capture, nutrient recovery, and bioproduct generation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104290"},"PeriodicalIF":4.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic engineering and metabolomics based profiling of cyanobacteria for enhanced succinate production","authors":"Meghna Srivastava ,&nbsp;Pramod P. Wangikar","doi":"10.1016/j.algal.2025.104286","DOIUrl":"10.1016/j.algal.2025.104286","url":null,"abstract":"<div><div>Succinate, a versatile four‑carbon dicarboxylic acid, is pivotal in synthesizing industrial chemicals, pharmaceuticals, and biodegradable polymers. Traditional production methods are constrained by intensive energy requirements, complex procedures, and reliance on non-renewable fossil resources, impeding sustainable manufacturing. This study introduces a robust and renewable production platform by integrating metabolic engineering with untargeted metabolomics in the fast-growing cyanobacterium <em>Synechococcus elongatus</em> PCC 11801. Specifically, we engineered heterologous expression of glyoxylate cycle enzymes, isocitrate lyase (ICL) and malate synthase (MS), under native cyanobacterial promoters to circumvent carbon loss typically encountered during decarboxylation in the TCA cycle. Concurrently, CRISPR-Cpf1 genome editing was utilized to knock out succinate dehydrogenase (SDH), which ordinarily diverts succinate toward fumarate, thus reinforcing succinate accumulation. High cell density cultivation in enriched 5× BG11 medium under optimized culture conditions with low light intensity and elevated CO₂ gave up to 350 mg/L extracellular succinate titer. Comprehensive metabolome profiling revealed that increased succinate production was associated with extensive reprogramming within central carbon metabolism, marked by enhanced glycolytic throughput, accumulation of TCA cycle intermediates, and pronounced changes in amino acid profiles and redox balance, but also imposed metabolic stress. These findings emphasize the effectiveness and promise of integrating genetic engineering with advanced metabolomic profiling and optimization of cultivation conditions to facilitate the sustainable photosynthetic production of succinate and other value-added chemicals.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104286"},"PeriodicalIF":4.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of including Arthrospira platensis as a functional ingredient in dairy cow feed","authors":"M. Barcelo-Villalobos ,&nbsp;F. Arrebola ,&nbsp;A. Vizcaino ,&nbsp;F.J. Alarcón ,&nbsp;F.G. Acién","doi":"10.1016/j.algal.2025.104295","DOIUrl":"10.1016/j.algal.2025.104295","url":null,"abstract":"<div><div>The sustainability of dairy production faces challenges due to environmental concerns and the high cost of conventional feed ingredients. <em>Arthrospira platensis</em>, a protein-rich cyanobacteria with bioactive compounds, has been proposed as a functional feed ingredient to enhance milk quality while supporting sustainable livestock practices. This study evaluates the effects of Arthrospira supplementation on milk production, nutritional composition, physicochemical properties, and animal health indicators, alongside its economic feasibility. A 35-day feeding trial was conducted on 12 Holstein dairy cows, each having a live body weight of 510–532 kg and 2–4 parities. They were divided into a control group and an Arthrospira-supplemented group (250 g/day, 1 % <em>w</em>/w of the feed ration). Milk production was monitored daily while milk composition (protein, fat, lactose, and solids-not-fat), physicochemical properties (cryoscopic point and electrical conductivity), bacterial content, somatic cell count (SCC), and milk urea nitrogen (MUN) were analysed weekly. Economic viability was assessed based on feed cost variations with the different Arthrospira levels included.</div><div>The results showed an initial reduction in milk yield due to feed rejection, which stabilized over time. Arthrospira supplementation did not significantly alter key milk components, but bacterial counts decreased at the beginning of the trial, suggesting antimicrobial benefits. The SCC and MUN remained within normal ranges, indicating no adverse effects on udder health or nitrogen metabolism. However, the economic analysis revealed a feed cost increase from €0.30/kg to €0.55/kg when 1 %<em>w</em>/w Arthrospira was included, highlighting the need for cost reduction strategies. This preliminary study suggests that <em>Arthrospira platensis</em> can serve as a sustainable functional feed ingredient, helping to maintain milk quality while offering antimicrobial benefits - though economic constraints limit large-scale adoption at present.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104295"},"PeriodicalIF":4.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in photobioreactor systems: Optimizing operations for enhanced microalgal growth and bioremediation","authors":"Zubair Hashmi ,&nbsp;Ibrahim Maina idriss ,&nbsp;Juliana Zaini ,&nbsp;Muhammad Saifullah Abu Bakar ,&nbsp;Muhammad Roil Bilad","doi":"10.1016/j.algal.2025.104282","DOIUrl":"10.1016/j.algal.2025.104282","url":null,"abstract":"<div><div>Photobioreactors (PBRs) are critical for sustainable production of biofuels, high-value compounds, and environmental services including carbon capture and wastewater treatment. This review compares tubular, flat-panel, vertical column, hybrid, and membrane-integrated PBRs, analyzing how geometry and operating strategies influence light utilization, mass transfer, nutrient delivery, and scalability. Recent performance data on illumination regimes, gas–liquid transfer rates, biomass productivity, and biochemical yields are synthesized to link engineering design with cultivation outcomes. Persistent challenges include oxygen accumulation, fouling, high energy demand, and scale-up limitations. Emerging solutions such as LED-based spectral control, modular hybrid configurations, and membrane-assisted biomass retention show strong potential. The review's distinctive contribution is a design-to-performance framework that connects reactor configuration and operational parameters to growth rates, product yields, and techno-economic viability. Future priorities include integrating computational fluid dynamics with kinetic growth models, developing low-energy gas transfer technologies, applying image-based monitoring for early contamination detection, and tailoring designs to local water, land, and regulatory conditions. By combining technical, environmental, and economic insights, this work provides a practical pathway toward cost-effective, scalable, and sustainable microalgae cultivation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104282"},"PeriodicalIF":4.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of endogenous phytohormone profilings in different life stages of Haematococcus pluvialis by targeted metabolomics","authors":"Yang Sun ,&nbsp;Yun Li ,&nbsp;Jing Zhang ,&nbsp;Jiaji Zhang ,&nbsp;Wenjie Yan ,&nbsp;Xu Gao","doi":"10.1016/j.algal.2025.104283","DOIUrl":"10.1016/j.algal.2025.104283","url":null,"abstract":"<div><div>Phytohormones play a crucial role in the growth and production of commercial microalgae, and are closely associated with their life stages. In the study, targeted metabolomics analysis was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) to investigate the phytohormone profiles of four life stages (zoospores, ZP; palmella, PL; palmella cell in transition to aplanospore, PAL; aplanospore, AL) of <em>Haematococcus pluvialis</em>. A total of 18 phytohormones were identified and quantified, including 4 auxins, 8 cytokinins (CKs), 2 jasmonates, 2 growth inhibitors, 1 abscisic acid (ABA), and 1 salicylic acid (SA). Indole-3-acetic acid (IAA) was the major auxin, with the highest content observed at the PL stage when biomass accumulation reaches its highest peak. <em>Cis</em>-zeatin (cZ) and <em>cis</em>-zeatin riboside (cZR) were the two predominant CKs, which increased significantly at the AL stage. Similarly, abscisic acid (ABA) content was the highest at the AL stage, which was associated with the accumulation of astaxanthin. The results provide important basic information for the development of plant growth regulators in the production of <em>H. pluvialis</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104283"},"PeriodicalIF":4.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell aggregation and temperature regulation mitigate Poterioochromonas predation on fast-growing cyanobacteria","authors":"Bin Long ,&nbsp;Ana Ramos ,&nbsp;Joshua S. Yuan ,&nbsp;Susie Y. Dai","doi":"10.1016/j.algal.2025.104285","DOIUrl":"10.1016/j.algal.2025.104285","url":null,"abstract":"<div><div>Cyanobacteria are promising for sustainable bioproduction but are vulnerable to predation, particularly by the mixotrophic protist <em>Poterioochromonas</em>, which threatens large-scale cultivation. This study investigates the predation susceptibility of three fast-growing cyanobacterial strains—<em>Synechococcus elongatus</em> UTEX 2973 (S2973), <em>Synechococcus elongatus</em> PCC 11801 (S11801), and <em>Synechococcus</em> sp. PCC 11901 (S11901)—to <em>Poterioochromonas</em> sp. CCMP 2740, using a reproducible predator-prey model. Grazing experiments, microscopy, and growth measurements revealed that while all strains were susceptible, S11801 and S11901 exhibited significantly higher resistance than S2973. This resistance was linked to their natural formation of microcolonies, which offered spatial protection and size exclusion against the predator. Engineering S2973 to aggregate enhanced its predation tolerance, confirming cell aggregation as a protective mechanism. Additionally, increasing temperature to 38 °C effectively eliminated the predator while supporting robust cyanobacterial growth, presenting a practical control strategy. These findings offer valuable insights for strain selection and predator management in cyanobacterial biomanufacturing, highlighting cell aggregation as an innate defense and temperature regulation as a practical control method.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104285"},"PeriodicalIF":4.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorella supplementation diminishes cardiovascular risk factors in adults: A GRADE-assessed systematic review and meta-analyses of randomized clinical trials","authors":"Hamidreza Shiri ,&nbsp;Abdolkarim Talebi Taheri ,&nbsp;Ali Akbar Soleimani ,&nbsp;Mohammad Hadi Nematollahi ,&nbsp;Reza Meshkani ,&nbsp;Ghodratollah Panahi ,&nbsp;Ozra Tabatabaei-Malazy ,&nbsp;Bagher Larijani","doi":"10.1016/j.algal.2025.104281","DOIUrl":"10.1016/j.algal.2025.104281","url":null,"abstract":"<div><h3>Background</h3><div>Cardiovascular disease (CVD) poses significant health challenges and life-threatening risks worldwide. Reducing risk factors for CVD has led to a decrease in its prevalence. Chlorella, a green alga, has beneficial cardiometabolic effects.</div></div><div><h3>Methods</h3><div>Systematic searches were performed in Web of Science, Scopus, and PubMed/Medline through January 10, 2025, to identify relevant randomized controlled trials (RCTs) according to the PRISMA checklist and PICOS criteria (Population: adults &gt;18 years, Intervention: chlorella, Comparison: placebo/control, Outcomes: BMI, weight, hs-CRP, FBG, HOMA-IR, insulin, TG, TC, HDL<img>C, LDL-C, SBP, DBP, and Study: RCTs). A meta-analysis was conducted using random-effects models. The GRADE framework was used to assess evidence certainty.</div></div><div><h3>Results</h3><div>In the 25 studies (33 effect sizes), chlorella supplementation improved dyslipidemia (TC: WMD = −5.26 mg/dl, <em>P</em> &lt; 0.001; LDL-C: WMD = −7 mg/dl, <em>P</em> &lt; 0.001; HDL<img>C: WMD = 2.34 mg/dl, <em>P</em> = 0.006), enhanced insulin sensitivity (HOMA-IR: WMD = −0.31, <em>P</em> = 0.004), lowered blood pressure (SBP: WMD = −3.68 mmHg, <em>P</em> = 0.005; DBP: WMD = −2.04 mmHg, <em>P</em> = 0.04), diminished anthropometric measures (weight: WMD = −1.37 kg, <em>P</em> = 0.001; BMI: WMD = −0.25 kg/m², <em>P</em> &lt; 0.001), and reduced inflammation (hs-CRP: WMD = −0.74 mg/dl, <em>P</em> &lt; 0.001). Chlorella supplementation did not affect TG, FBG, and insulin levels. GRADE analysis showed low-quality evidence for hs-CRP and FBG, high-quality evidence for TC, LDL-C, and BMI, and moderate-quality evidence for other parameters.</div></div><div><h3>Conclusions</h3><div>This systematic review and meta-analysis demonstrate that chlorella supplementation improves blood pressure, anthropometric measures, insulin sensitivity, inflammatory markers, and dyslipidemia, which may help lower CVD risk in adults. However, more studies are needed to confirm these facts.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104281"},"PeriodicalIF":4.5,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic complexity and evolutionary diversification of a novel cyanobacterium from coral reef ecosystems","authors":"Haiyan Li ,&nbsp;Wenjie Shi ,&nbsp;Leyao Zeng ,&nbsp;Jianhua Kang ,&nbsp;Kieng Soon Hii ,&nbsp;Hala F. Mohamed ,&nbsp;Renhui Li ,&nbsp;Zhaohe Luo","doi":"10.1016/j.algal.2025.104280","DOIUrl":"10.1016/j.algal.2025.104280","url":null,"abstract":"<div><div>Heterocyte-forming cyanobacteria play a critical role in carbon, oxygen, and nitrogen cycling in coral reef ecosystems. Despite their ecological significance and biomedical potential, our understanding of reef-associated cyanobacteria remains limited. This study described <em>Aliinostoc maniaoense</em> sp. nov., a novel heterocyte-forming cyanobacterium isolated from a tropical coral reef in the South China Sea. Morphologically, <em>A. maniaoense</em> exhibited filaments with loose structural arrangement, gas vesicle-bearing motile hormogonia, and cylindrical or irregularly shaped akinetes, distinguishing it from other members of the genus. Phylogenetic analyses confirmed its placement within <em>Aliinostoc</em> and demonstrated significant genetic divergence from previously described species. Genomic analysis revealed a complete nitrogen fixation gene cluster, whose phylogeny suggested ancient rearrangement or horizontal acquisition, likely reflecting ecological adaptation and the selective advantage of biological nitrogen fixation in nutrient-poor coral reef habitats. Additionally, <em>A. maniaoense</em> possessed an unusually high number of ribosomal operons, with notable variations in 16S<img>23S internal transcribed spacer (ITS) secondary structures, highlighting the complexity of ITS-based taxonomy in cyanobacteria. In vitro bioactivity assays demonstrated potent cytotoxic effects against several cancer cell lines, underscoring its biomedical potential. Moreover, genome mining revealed biosynthetic gene clusters for nocuolin A and heterocyst glycolipids along with several uncharacterized secondary metabolite pathways, suggesting promising ecological and biotechnological applications. These findings expanded our understanding of cyanobacterial diversity in coral reefs and emphasized the importance of integrating morphological, molecular, and genomic approaches for accurate species classification and functional characterization.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104280"},"PeriodicalIF":4.5,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct mRNA and miRNA responses in Chlamydomonas reinhardtii reveal particle-specific adaptive mechanisms to ZnO nanoparticles and ZnO bulk","authors":"Monika Damyanov ,&nbsp;Martina Kolackova ,&nbsp;Vladimira Tarbajova ,&nbsp;Zuzana Bytesnikova ,&nbsp;Nikola Vintrlikova ,&nbsp;Pavel Svec ,&nbsp;Jana Pekarkova ,&nbsp;Petr Urbis ,&nbsp;Jana Havrankova ,&nbsp;Samuel Zachej ,&nbsp;Dominik Pinkas ,&nbsp;Vlada Filimonenko ,&nbsp;Lukas Richtera ,&nbsp;Andrea Ridoskova ,&nbsp;Dalibor Huska","doi":"10.1016/j.algal.2025.104266","DOIUrl":"10.1016/j.algal.2025.104266","url":null,"abstract":"<div><div>We investigated the adaptive responses of <em>Chlamydomonas reinhardtii</em> to ZnO nanoparticles (ZnO NPs) and ZnO bulk (ZnO Bulk) using integrated physiological, transcriptomic, and miRNA analyses. Cryo-TEM and AAS showed that ZnO NPs accumulated at higher levels and were more widely distributed in cells, whereas ZnO Bulk formed larger intracellular structures. Both materials activated oxidative stress defenses and metal homeostasis, but Bulk uniquely induced strong DNA repair, protein folding, and cell cycle pathways, indicating sustained cellular stress. Small RNA profiling revealed broader miRNA modulation under Bulk exposure, targeting genes linked to chromatin regulation, signaling, and stress adaptation. However, the absence of consistent inverse miRNA–mRNA correlations suggests that miRNAs act mainly as modulators, with transcriptional and proteomic reprogramming driving the primary response. These findings highlight how particle size and aggregation state shape both transcriptional and post-transcriptional regulation in algal stress adaptation, providing new insight into microalgal resilience to engineered ZnO materials.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104266"},"PeriodicalIF":4.5,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot green synthesis of platinum nanoparticles (Pt-NPs) using Sargassum polyphyllum extract displays nanodrug prospects against human cervical, breast, and colon cancer cells","authors":"Sabiha Mahmood Ansari ,&nbsp;Quaiser Saquib ,&nbsp;Hend Awad Alwathnani ,&nbsp;Sulaiman Ali Alharbi ,&nbsp;Mohammed Rafi Shaik ,&nbsp;Mufsir Kuniyil ,&nbsp;Syed Farooq Adil ,&nbsp;Maqsood Ahmad Siddiqui ,&nbsp;Javed Ahmad ,&nbsp;Rizwan Wahab ,&nbsp;Sheikh Fayaz Ahmad ,&nbsp;Abdulaziz Ali Al-Khedhairy","doi":"10.1016/j.algal.2025.104278","DOIUrl":"10.1016/j.algal.2025.104278","url":null,"abstract":"<div><div>Fabrication of nanoparticles (NPs) via green method has superseded the chemical procedures owing to its inherent features of biocompatibility and environment safety. From this perspective we used <em>Sargassum polyphyllum</em>, a marine brown macroalgae, preserving unique phytochemicals and growing under stress-tolerant environmental settings. We aimed for synthesizing platinum NPs (Pt-NPs) using extracellular extract of <em>S. polyphyllum</em> and evaluated its in vitro anticancer efficacy against human breast (MCF-7), cervical (HeLa), and colon (HT-29) cancer cells. Pt-NPs formation confirmed by color change with simultaneous disappearance of SPR band at 258.4 nm. TEM and SEM analysis exhibited rough surfaced aggregated clusters of pleomorphic spheroidal particles having an average size of 4.7 ± 0.01 nm. The hydrodynamic properties of Pt-NPs showed small (90.65 d.nm) and large (367.7 d.nm) peaks in DLS and a zeta (<em>ζ)-</em>potential of −43.6 mV. MTT and NRU assays exhibited cytotoxic effects in MCF-7, HeLa, and HT-29 cells after Pt-NPs exposure for 24 h. Flow cytometric analysis of Pt-NPs-treated cells caused dysfunction of mitochondrial membrane potential (<em>ΔΨm</em>), greater levels of intracellular ROS, NO, esterase, and Ca²⁺ influx. Pt-NPs induced nuclear DNA damage and triggered apoptotic cell death in the cancer cells. Subsequently immunofluorescence has affirmed cytoplasmic localization of p53 in Pt-NPs treated cancer cells. qPCR array of 84 genes demonstrated the activation of myriad genes pertaining to human cancer pathway in the exposed cells. On the whole Pt-NPs has demonstrated its anticancer efficacy in the order of HeLa&gt;MCF-7 &gt; HT-29 cells indicating its prospective as a nanodrug derived through eco-friendly approach.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"91 ","pages":"Article 104278"},"PeriodicalIF":4.5,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}