Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Scale-up study of enhancing algal growth through bioaugmentation with the indole-3-acetic acid producing bacteria Azospirillum brasilense","authors":"Reilly Durham ,&nbsp;Linduo Zhao ,&nbsp;Vanessa DeShambo ,&nbsp;Grace Wilken ,&nbsp;Trevor Rickerd ,&nbsp;Devin Epstein ,&nbsp;Lance Charles Schideman ,&nbsp;Sungwhan Kim ,&nbsp;Jihoon Yang ,&nbsp;Ryan W. Davis","doi":"10.1016/j.algal.2025.104163","DOIUrl":"10.1016/j.algal.2025.104163","url":null,"abstract":"<div><div>Algae-based wastewater treatment technologies can recover nutrients while generating algal biomass with diverse application potentials. While numerous bacteria have demonstrated the ability to enhance algal growth through symbiotic relationships in laboratory settings, scaling this benefit to larger applications remains challenging due to complex wastewater conditions and microbe-algae dynamics. This study explores the effectiveness of bioaugmentation with <em>Azospirillum brasilense</em>, a plant growth-promoting bacterium known for producing the phytohormone indole-3-acetic acid (IAA), in boosting algal productivity within an Algaewheel wastewater treatment system. Through a series of experiments conducted at lab-, pilot-, and full-scale levels, we evaluated the impact of bioaugmentation on algal growth and developed a bioaugmentation strategy for an Algaewheel system. The results indicate that a biweekly dosing, achieving a final bioaugmenting cell density of 0.2–4 × 10⁹ cells/L, effectively doubles the biomass productivity in a residential subdivision wastewater treatment plant in Northern Illinois. The bacterial production of IAA was the primary mechanism driving this enhancement, as demonstrated by the similar growth and yield improvements observed with chemically supplemented IAA and bioaugmentation across all experimental scales. High-throughput sequencing of 18S rRNA genes revealed that the algal community in the bioaugmented tank exhibited more stable biodiversity than the control. Additionally, bioaugmentation improved nutrient removal efficiency during winter, highlighting its potential to enhance the overall performance and sustainability of algae-based wastewater systems. The developed bioaugmentation and chemical treatment methods offer further operational solutions for managing yield, optimizing biochemical profiles, and enhancing biofuel production potential.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104163"},"PeriodicalIF":4.6,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular phylogeny and character evolution of family Selenastraceae (Sphaeropelales, Chlorophyceae, Chlorophyta)","authors":"Xudong Liu ,&nbsp;Xiaoqian Duan ,&nbsp;Na Ma ,&nbsp;Jia Feng ,&nbsp;Zhengyu Hu ,&nbsp;Shulian Xie","doi":"10.1016/j.algal.2025.104164","DOIUrl":"10.1016/j.algal.2025.104164","url":null,"abstract":"<div><div>The family Selenastraceae comprises a group of very common small green algae which have extensive application value across various economic sectors. However, the taxonomic status of the family is highly confused, and the phylogenetic relationships among the major taxa within the family remain unclear. In this study, we obtained 90 new algal strains from across China, identified 24 species and a large number of undetermined species, and added 167 new molecular sequences, significantly expanding the data volume for this family. The phylogenetic positions of genus <em>Apodococcus</em> and eight species were determined for the first time. Based on more reliable phylogenetic relationships, we systematically organized and discussed the existing and newly discovered polyphyly issues of the genera <em>Ankistrodesmus</em>, <em>Monoraphidium</em>, <em>Kirchneriella</em>, <em>Raphidocelis</em> and <em>Nephrochlamys</em>. We also proposed that the genus <em>Ankistrodesmus</em> may represent an early-evolving lineage within the family. Furthermore, ancestral reconstruction analysis indicated that fusiform cell shape, unicellular or small-colony, parallel arrangement of autospores, release of autospores via median split of mother cell wall, naked pyrenoids, and a thin mucilaginous envelope were plesiomorphies within the family. In the future, the acquisition of additional algal strains and molecular data will help to construct more stable phylogenetic relationship among taxa and further complete the taxonomic revision of the family Selenastraceae.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104164"},"PeriodicalIF":4.6,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective cyanobactericidal activity of Nocardioides convexus MS16 against Microcystis aeruginosa, mediated by direct attack","authors":"Seonah Jeong ,&nbsp;Ve Van Le ,&nbsp;So-Ra Ko ,&nbsp;Mingyeong Kang ,&nbsp;Min-Seong Kim ,&nbsp;Zhun Li ,&nbsp;Chi-Yong Ahn","doi":"10.1016/j.algal.2025.104165","DOIUrl":"10.1016/j.algal.2025.104165","url":null,"abstract":"<div><div>A cyanobactericidal bacterium, <em>Nocardioides convexus</em> MS16, was isolated from the co-culture solution of <em>Microcystis aeruginosa</em> and <em>Myriophyllum spicatum</em> (Eurasian watermilfoil), an aquatic plant known for its inhibitory effects on the growth of <em>M. aeruginosa</em>. The present study investigated the cyanobactericidal activity of <em>N. convexus</em> MS16 against <em>M. aeruginosa</em> KW, evaluating its potential as a biocontrol agent for managing <em>Microcystis</em> blooms. To determine the algicidal mode of action, treatments using the bacterial culture, washed cells (direct attack), and cell-free filtrate (indirect allelopathy) were compared. The MS16 strain exhibited a weak allelochemical impact but a strong direct physical attack, demonstrating algicidal activity of 84 % against <em>M. aeruginosa</em> KW within a day and 98 % within two days, using washed cells. The scanning electron microscope images showed that the cells of <em>M. aeruginosa</em> KW were damaged by cell surface-attached <em>N. convexus</em> MS16. Furthermore, <em>N. convexus</em> MS16 exhibited a selective inhibition of <em>M. aeruginosa</em>, compared to other cyanobacteria and green algae. The gene expression levels of <em>ftsH</em>, <em>rbcL</em>, <em>mcyA</em>, <em>SOD</em>, <em>GSH</em>, and <em>recA</em> in <em>M. aeruginosa</em> KW significantly decreased after treatment with <em>N. convexus</em> MS16 culture, indicating that <em>M. aeruginosa</em> had lost the ability to grow and survive. These findings highlight the potential of <em>N. convexus</em> MS16 to enhance the development of eco-friendly methods to mitigate harmful cyanobacterial blooms.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104165"},"PeriodicalIF":4.6,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of N-Methylpyrrolidone (NMP) with a rotating algae-bacterial biofilm system","authors":"Zhongyi An,&nbsp;Pengqiang Song,&nbsp;Dan Li,&nbsp;Xiuwen You,&nbsp;Huichao Zhang","doi":"10.1016/j.algal.2025.104161","DOIUrl":"10.1016/j.algal.2025.104161","url":null,"abstract":"<div><div>N-Methyl-2-pyrrolidone (NMP) is widely used in the lithium battery manufacturing industry, and residual NMP is discharged with production wastewater. Traditional physicochemical methods for treating NMP-containing wastewater face challenges such as high energy consumption, complex operations, and environmental risks. Considering NMP as a nitrogenous heterocyclic compound, this research investigated the potential of an algal-bacterial symbiotic system for the effective treatment of NMP wastewater. In this treatment approach, NMP was used as the exclusive source of nitrogen and carbon in the wastewater. The results demonstrated that in sequential batch experiments with NMP concentrations of 1000 mg/L and 4000 mg/L, removal efficiencies of COD, TOC, and TN all exceeded 95 %. Ammonium nitrogen produced from NMP degradation was efficiently absorbed by the algae within the biofilm, ensuring that the treated effluent met the wastewater discharge standards of the lithium battery industry. In semi-continuous flow experiments, maintaining a HRT of 72 h resulted in average removal efficiency of COD, TOC, and TN for 1000 mg/L NMP wastewater at 70.9 ± 3.7 %, 92.4 ± 0.7 %, and 37.8 ± 8.9 %, respectively. Microbial community analysis revealed that the presence of NMP enhanced the species richness within the biofilm. Heterotrophs <em>Chitinophagales</em>，<em>Phormidesmiales</em> were the most abundant bacteria, likely contributing to TOC degradation, while autotrophs <em>Phormidesmiales</em> and <em>Trebouxiophyceae, Chlorella, Chloroflexales, Chloronema</em> potentially playing a role in ammonium nitrogen absorption and fixation. The presence of protists and micro-metazoans such as <em>Amphileptus, Rhabditida</em> and <em>Phascolodon</em>, within the biofilms demonstrates a more complete biological chain within the biofilms, which contributes to the removal of pollutants from the wastewater. These insights offer a novel biological treatment technology for the efficient management of lithium battery wastewater.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104161"},"PeriodicalIF":4.6,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brown alga Costaria costata as a source of phlorethol that inhibits TPA-induced neoplastic cell transformation and progression of human breast cancer cells via AKT/GSK-3β/CDKs pathway","authors":"Olesya S. Malyarenko,&nbsp;Tatiana I. Imbs,&nbsp;Roza V. Usoltseva,&nbsp;Svetlana P. Ermakova","doi":"10.1016/j.algal.2025.104162","DOIUrl":"10.1016/j.algal.2025.104162","url":null,"abstract":"<div><div>Phlorotannins are polyphenolic compounds synthesized by brown algae that have recently attracted great interest due to their unique structure, vast range of bioactivities, and potential applications in human health and nutrition. In the present study, the chemical structure of phlorethol from the brown alga <em>Costaria costata</em> (CcPh) is elucidated. Its effect and molecular mechanisms of anticancer action are assessed using the models of 12-<em>O</em>-tetradecanoylphorbol-13-acetate (TPA)-induced tumorigenic transformation of mouse epidermal JB6 Cl41-5a cells and human triple-negative breast cancer MDA-MB-231 cells. It has been found that CcPh having a degree of polymerization of 12–25 phloroglucinol units and a molecular weight of 2520 Da, belong to the phlorethol class. CcPh shows cytotoxic activity against non-induced and TPA-induced JB6 Cl41-5a cells with IC₅₀ = 97.1 ± 3.0 and 114.3 ± 2.2 μg/mL, respectively, or MDA-MB-231 cells with IC₅₀ = 95 ± 1.2 and 109 ± 7.5 μg/mL, respectively. CcPh effectively inhibits TPA-induced neoplastic transformation of JB6 Cl41-5a cells, colony formation, and migration of MDA-MB-231 cells through the inhibition of AKT and GSK-3β kinase phosphorylation and the regulation of cell cycle protein (CDK4, Cyclin D1, and p21) expression. These properties of phlorotannins provide a wide range of opportunities for the development of new approaches to prevention and therapy of highly aggressive forms of breast cancer in the future.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104162"},"PeriodicalIF":4.6,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144535593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diesel alkanes are incorporated and metabolized in Ulva lactuca activating the expression and activities of antioxidant enzymes and alkane-metabolizing enzymes","authors":"Stephanie Romero ,&nbsp;Patricia Méndez ,&nbsp;Rodrigo A. Contreras ,&nbsp;Marlén Gutiérrez-Cutiño ,&nbsp;Héctor Osorio ,&nbsp;Alberto González ,&nbsp;Alejandra Moenne","doi":"10.1016/j.algal.2025.104166","DOIUrl":"10.1016/j.algal.2025.104166","url":null,"abstract":"<div><div>To analyze whether the marine alga <em>Ulva lactuca</em> can incorporate and metabolize linear hydrocarbons (alkanes) of diesel, the alga was cultivated without diesel (control) or with 2.5 % v/v of diesel for 168 h (7 d). The concentration of alkanes in the culture medium decreased by 83 % at 168 h of culture while in algal tissue alkane levels increased to a maximal level at 24 h and decrease in 78 % at 168 h of culture. Diesel was constituted by alkanes from C7 to C28, mainly by alkanes C14 to C21 which represents 73 % of total alkanes. In algal tissue, the concentration of alkanes showed a maximal level at 24 h and alkanes C13 and C14 decreased in 100 % at 168 h, alkanes C15 and C16 decreased in 98 and 92 %, respectively at 168 h, and alkanes C18 and C21 decreased around 60 %. Thus, <em>U. lactuca</em> probably metabolizes main diesel alkanes in 60 % to 100 % at 168 h of culture. The incorporation of alkanes induced an oxidative stress condition characterized by an increase in superoxide anions and hydrogen peroxide levels that was rapidly mitigated by the increase in the level of antioxidant molecules such as ascorbate and glutathione, and the increase in activities of antioxidant enzymes such as SOD, AP, DHAR and GR showing maximal activities at 24 h of exposure. The expression and activities of alkane-metabolizing enzymes such as alkane monooxygenase, alcohol dehydrogenase and aldehyde dehydrogenase were increased, indicating that they may be involved in the metabolization of alkanes into fatty acids to fuel basal metabolism. Thus, the alga <em>U. lactuca</em> may constitute a useful biotechnological tool to remove diesel from seawater.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104166"},"PeriodicalIF":4.6,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of properties of seaweed-based films by fatty acid incorporation: Preservation potential for cherry tomatoes","authors":"Zahide Polat ,&nbsp;Huriye Gözde Ceylan ,&nbsp;Ahmet Ferit Atasoy","doi":"10.1016/j.algal.2025.104159","DOIUrl":"10.1016/j.algal.2025.104159","url":null,"abstract":"<div><div>This study aimed to enhance the properties of seaweed (<em>Chondrus crispus</em>) mucilage-based films by incorporating stearic acid (SA) at concentrations of 0, 5, 10, 15, and 20 % (<em>w</em>/w, based on polymer weight). The fabricated films were characterized for their physicochemical, mechanical, structural, and optical properties. Furthermore, the film-forming solution (containing 0 % and 20 % SA) was used to coat cherry tomatoes, and their quality characteristics were analyzed during 12 days of storage at 10 °C. SA incorporation led to significant (<em>p</em> &lt; 0.05) improvements in water vapor barrier capacity (WVP) and elasticity (EAB), with concentrations above 10% showing comparable values for both properties (<em>p</em> &gt; 0.05). Films with 5 % SA showed weak oxygen barrier properties, while films with higher SA concentrations demonstrated similar performance (<em>p</em> &gt; 0.05) to the control. Moreover, increasing SA concentration led to a significant decrease in light transmittance. SEM analysis revealed that SA caused layering and lipid aggregation. Furthermore, the addition of SA resulted in the formation of new bands in XRD and changes in band intensities and positions in FTIR. Coating treatments limited the changes in the total soluble solids content, pH, and titration acidity of cherry tomatoes compared to the uncoated sample. While the matrix containing 20 % SA effectively preserved the quality of the cherry tomatoes, the SA-free coating (SA0) resulted in greater weight loss and softening compared to the uncoated tomatoes. In conclusion, SA incorporation enhances the mechanical, light barrier, and structural properties of seaweed-based films, and the 20 % SA film matrix is an alternative for preserving cherry tomatoes.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104159"},"PeriodicalIF":4.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of phytoplankton groups using in-situ multi-excitation chlorophyll fluorescence measurements and machine learning (mf-ML)","authors":"Qinglong Zhang ,&nbsp;Yan Huang ,&nbsp;Soonmo An","doi":"10.1016/j.algal.2025.104155","DOIUrl":"10.1016/j.algal.2025.104155","url":null,"abstract":"<div><div>This study introduces a novel framework for the high-resolution quantification of phytoplankton communities using machine learning, integrating <em>in situ</em> multi-excitation chlorophyll fluorescence data with traditional high-performance liquid chromatography (HPLC) methods. The framework overcomes limitations of conventional sampling techniques by providing continuous, high-resolution profiling of phytoplankton distributions, capturing diel vertical migration (DVM) patterns, and analyzing responses to environmental factors such as irradiance and nutrient concentrations. The results from an XGBoost (Extreme Gradient Boosting)-based model demonstrated strong predictive accuracy across eight phytoplankton groups, effectively identifying complex nonlinear interactions that traditional methods struggle to resolve. Specifically, the model successfully traced DVM in dinoflagellates, offering insights into harmful algal bloom (HAB) dynamics. This study highlights multi-wave length excited fluorescence spectrometry as a cost-effective, accurate, and robust tool for monitoring phytoplankton distributions <em>in situ</em>, offering a significant advancement over remote sensing and discrete sampling techniques. By providing continuous monitoring of phytoplankton behavior and community structure, this approach can enhance the management of marine ecosystems, particularly in the context of HABs. Future work could expand this framework's applicability to other marine regions and phytoplankton communities, with the potential for real-time monitoring systems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104155"},"PeriodicalIF":4.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics insights of biotin-enhanced astaxanthin and total fatty acid productivity in heterotrophic Chromochloris zofingiensis under stress conditions","authors":"Haodong Wang ,&nbsp;Xiaoshuang Zhang ,&nbsp;Min Gao ,&nbsp;Shixuan Gong ,&nbsp;Yaqi Xu ,&nbsp;Jiayin Wang ,&nbsp;Man Zhang ,&nbsp;Mengyao Xu ,&nbsp;Tiantian Zhao ,&nbsp;Zhao Zhang","doi":"10.1016/j.algal.2025.104158","DOIUrl":"10.1016/j.algal.2025.104158","url":null,"abstract":"<div><div>Astaxanthin is a secondary carotenoid which predominantly accumulates under abiotic stresses at the expense of cell growth. Functioning as an essential cofactor for carboxylase enzymes, biotin has been demonstrated to significantly enhance cellular stress resistance. This study explores the effects of exogenous biotin supplementation on the growth, astaxanthin, and total fatty acid (TFA) production in <em>Chromochloris zofingiensis</em> under nitrogen deficiency (ND) and high salt stress (HS). Exogenous biotin significantly improved biomass accumulation with the reduced reactive oxygen species (ROS) level under both stresses. At a concentration of 1000 μg L⁻¹, the maximal dry weight (DW) and astaxanthin content were achieved, which resulted in the highest astaxanthin yield as 3.29-fold and 2.61-fold of that in the respective controls under ND and HS conditions. Besides, biotin also enhanced TFA yield by 54 % and 36 % under ND and HS conditions, respectively. Integrated transcriptomic and metabolomic analyses demonstrated that biotin significantly enriched pathways related to amino acid metabolism, lipid, and carbohydrate metabolism under ND; whereas pathways associated with energy metabolism and carotenoid biosynthesis under HS. Biotin modulated the expression of key genes in carotenoid biosynthesis (<em>PSY</em>, <em>PDS</em>, <em>ZDS</em>, <em>CrtZ</em>) and fatty acid biosynthesis (<em>FabH</em>, <em>FabG</em>), demonstrating its role in fine-tuning these metabolic pathways. This study establishes biotin as a potent regulator of simultaneous astaxanthin and fatty acid accumulation in <em>C. zofingiensis</em> under stress conditions.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104158"},"PeriodicalIF":4.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrolysis and characterization of bioactivity and functionality properties of protein fractions from Spirulina platensis and Sargassum illicifolium for active shrimp preservation","authors":"Zahra Soltani Far ,&nbsp;Haidar sattar Abdoulwahid almashkoor ,&nbsp;Davood Abbaszadeh Silakhori ,&nbsp;Mahdieh Doraj","doi":"10.1016/j.algal.2025.104144","DOIUrl":"10.1016/j.algal.2025.104144","url":null,"abstract":"<div><div>Marine algae are promising sources of bioactive proteins, but their functional potential remains underexplored. Developing natural preservatives for seafood (e.g., shrimp) is critical to replace synthetic additives and address spoilage during cold storage. This study aimed to (1) produce bioactive peptides from <em>Spirulina platensis</em> and <em>Sargassum ilicifolium</em> via enzymatic hydrolysis using Alcalase, Protamex, Pancreatin, Pepsin, and Bromelain, followed by fractionation through membrane ultrafiltration, (2) characterize their functional/antioxidant/antibacterial properties, and (3) evaluate their efficacy in chitosan-starch coatings for preserving shrimp (<em>Litopenaeus vannamei</em>). Pepsin and Pancreatin yielded the highest degrees of hydrolysis (DH) for <em>S. platensis</em> (24.85 %) and <em>S. ilicifolium</em> (25.25 %). These fractions exhibited excellent solubility (&gt;80 % at pH 9), foaming (up to 46.22 %), emulsifying (EAI: 22.69 m²/g), and antioxidant properties (DPPH/ABTS scavenging and metal chelating activity). Sp- &lt; 3 kDa inhibited L. <em>monocytogenes</em> (12.3 mm zone) and <em>E. coli</em> (9.1 mm), while Si- &lt; 3 kDa was effective against <em>S. typhimurium</em> (6.2 mm). Coatings with Sp- &lt; 3 kDa significantly reduced TVB-N (18.88 vs. 34.95 mg N/100 g in control), microbial counts (TVC: 7.07 vs. 8.51 log CFU/g), and lipid oxidation (PV: 1.86 vs. 2.97 mil eq. O₂/kg) after 9 days at 4 °C (<em>P</em> &lt; 0.05). The &lt;3 kDa fractions demonstrated dual antioxidant-antimicrobial effects, offering a natural alternative for active food packaging.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"90 ","pages":"Article 104144"},"PeriodicalIF":4.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}