Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Worldwide distribution, current trends and scientific progress in Spirulina research (1967–2024): A Scientometric analysis","authors":"Michele Corrêa Bertoldi ,&nbsp;Monique Ellen Torres Resende ,&nbsp;Joyce Késsia Alves Grigório ,&nbsp;Hernani Ciro Santana","doi":"10.1016/j.algal.2025.104029","DOIUrl":"10.1016/j.algal.2025.104029","url":null,"abstract":"<div><div>Spirulina is a promising functional food with versatile applications in the environment, animal feed, cosmetics, biofuel production, agriculture, biotechnology, and medicine. This study presents the first scientometric analysis of the scientific literature concerning Spirulina published worldwide in the Web of Science database (1967–2024). The descriptive and network/visualization analyses were carried out via WoS tools and CiteSpace, respectively. It presents a visualization of complex information (5899 articles) from a broad perspective. The number of publications increased 11.3 times from 2002 to 2022, whereas the average number of citations per year increased by 32.3 times in the same period. Most academic production of Spirulina has been carried out by researchers from China (15.1 % of the world production), India (11.6 %), the USA (8.2 %), Brazil (8.0 %) and Egypt (6.5 %). Over the past twenty years, research on Spirulina has advanced significantly, revealing remarkable progress in understanding its health benefits and applications for food and dietary supplementation purposes. Spirulina shows promise for modulating the gut microbiota, but robust human clinical studies are lacking. Even though spirulina is a potential source of biodiesel, high production costs still hinder its commercial viability. Spirulina production has a significant positive impact on waste reduction, bioremediation and economic diversification. While Spirulina production continues to present growing opportunities across various sectors, from health to energy, there is a clear need to overcome gaps related to scalability, consumer acceptance, lifecycle assessments, engine performance, economic feasibility and regulation to maximize its positive impact on the circular economy and global sustainability.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104029"},"PeriodicalIF":4.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of salt concentration and trophic mode on glucosylglycerol and phycocyanin production by Arthrospira maxima","authors":"Jianke Huang ,&nbsp;Ting Yao ,&nbsp;Sanjiong Yi ,&nbsp;Dawei Sun ,&nbsp;Pan Zhang ,&nbsp;Hanlong Wang ,&nbsp;Xiwen Xue ,&nbsp;Ruizeng Zhang","doi":"10.1016/j.algal.2025.104025","DOIUrl":"10.1016/j.algal.2025.104025","url":null,"abstract":"<div><div><em>Arthrospira maxima</em> is an excellent cyanobacterial strain for phycocyanin production, while it has also recently been employed to produce a high-value product called glucosylglycerol that can be used as a cosmetic and food ingredient. In this study, the effect of NaCl-induced salt stress on cell growth, glucosylglycerol (GG) and phycocyanin (PC) production by <em>A. maxima</em> was investigated. The results suggested that the addition of NaCl effectively induced GG synthesis in cyanobacteria. The GG content increased from 0 % to the highest level of 15.29 % with increasing NaCl concentrations from 0 to 900 mM. However, the highest GG yield (174.54 mg L⁻¹) was obtained at 600 mM NaCl as a higher NaCl concentration significantly reduced the biomass concentration. Furthermore, the PC content decreased from 17.44 to 9.72 %, and the PC yield sharply reduced from 333.63 to 80.00 mg L⁻¹, indicating that increasing salt concentration adversely affected PC production. Consequently, the NaCl concentration of 300 mM was considered a suitable option for simultaneous production of PC and GG, under which both the high yields of GG (149.00 mg L⁻¹) and PC (249.05 mg L⁻¹) were achieved. Moreover, trophic mode markedly influenced GG synthesis in <em>A. maxima.</em> Photoautotrophic cells accumulated as much as 15.77 % GG in cyanobacterial cells, whereas only 6.28 % GG accumulated in mixotrophic cells, resulting in a significantly lower GG yield. In conclusion, the application of salt stress was an effective approach to producing GG under the photoautotrophic mode, and a medium salt concentration was the optimal option to simultaneously achieve high productivities of GG and PC. This study lays a foundation for efficient co-production of PC and GG, facilitating the high-value utilization of <em>Arthrospira maxima.</em></div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104025"},"PeriodicalIF":4.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated approach for wastewater remediation and biofertilizer development using Chlorella vulgaris in raceway ponds","authors":"Daniela Delgado Pineda ,&nbsp;Mery Y. Flores Guetti ,&nbsp;Ximena Rosado-Espinoza ,&nbsp;Muhammad Ajaz Ahmed ,&nbsp;Gilberto J. Colina Andrade ,&nbsp;Ruly Terán Hilares","doi":"10.1016/j.algal.2025.104022","DOIUrl":"10.1016/j.algal.2025.104022","url":null,"abstract":"<div><div>Wastewater is quite complex and requires a series of different processes for its treatment. Vermifilters are one such strategy that significantly reduces pollutants but with limited ability to remove phosphorus and nitrogen. To address this, the effluent from the vermifilters can be further processed by microalgae, which can enhance water quality. In this study, microalgae were cultivated in an outdoor raceway pond, using treated wastewater as medium, and the resulting biomass was used as a biofertilizer for Cherry tomato (<em>Solanum lycopersicum</em> var. <em>cerasiforme</em>) cultivation. The microalgae treatment led to significant reductions of 78 % in COD, 86 % in phosphorus, and 45 % in nitrogen (<em>p</em> &lt; 0.05). A microalgae concentration of 1.45 g/L was achieved, with a productivity rate of 0.43 g/L/day. The microalgae biomass contained 56.17 % protein, 30.73 % carbohydrates, and 18 % lipids. After 90 days of growth, cherry tomato plants fertilized with the microalgae-based biofertilizer produced a total weight of 622.4 g, compared to 756.5 g with chemical NPK fertilizer (20-20-20) and 285.4 g with no fertilizer (the control). In terms of other growth metrics such as average height, dry biomass, and number of leaves; the plants treated with commercial fertilizer reached the best results, following by the microalgae biofertilizer and finally, the control showed the least growth. These results suggest that microalgae-based biofertilizer can be a promising alternative, particularly for sandy loam soils, which are usually employed for tomato cultivation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104022"},"PeriodicalIF":4.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the of red seaweed polysaccharides on hypertension–associated biomarkers in cadmium chloride-induced Wistar rats","authors":"Chaithanya Bose ,&nbsp;Penchala Udaya Bhanu ,&nbsp;Kajal Chakraborty ,&nbsp;Lokanatha Valluru ,&nbsp;Shubhajit Dhara ,&nbsp;Shilpa Kamalakar Pai","doi":"10.1016/j.algal.2025.104021","DOIUrl":"10.1016/j.algal.2025.104021","url":null,"abstract":"<div><div>Red seaweeds, recognized for their diverse polysaccharide structures, exhibit significant therapeutic potential in modulating the renin-angiotensin-aldosterone system (RAAS) to mitigate hypertension, a major risk factor for cardiovascular diseases. This study investigates the antihypertensive properties of polysaccharides extracted from four Rhodophyta species: <em>Gracilaria salicornia</em> (GSP), <em>Gracilaria edulis</em> (GEP), <em>Gelidiella acerosa</em> (GAP), and <em>Gelidium pusillum</em> (GPP), using a cadmium chloride (CdCl₂)-induced hypertensive model in Wistar rats. GSP (100 mg/kg BW) significantly reduced hypertension-associated biomarkers, including angiotensin II (&gt;65 %), C-reactive protein and myoglobin (&gt;54 %), and troponin I and T (∼72 %), compared to GEP, GAP, and GPP in hypertensive rats (<em>p</em> &lt; 0.05). GSP also improved serum levels of vitamin E (0.15 μg/mL) and nitric oxide (7.31 μg/mL), comparable to the telmisartan group, which restored vitamin E and nitric oxide to 0.24 μg/mL and 8.27 μg/mL, respectively. Antioxidant assays revealed a significant reduction in enzymatic antioxidant activity in CdCl₂-induced hypertensive rats relative to the healthy control group, with notable recovery upon GSP treatment. Histopathological evaluation indicated restored structural integrity of extracellular and cellular components in GSP-treated hypertensive rats, highlighting its potential to alleviate hypertension-induced oxidative stress.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104021"},"PeriodicalIF":4.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiochemical insights into substrate-diatom adhesion: Influence of surface properties on biofilm dynamics and productivity","authors":"Manikandan Sivakumar ,&nbsp;Inbakandan Dhinakarasamy ,&nbsp;Clarita Clements ,&nbsp;Naren Kumar Thirumurugan ,&nbsp;Subham Chakraborty ,&nbsp;Anu Chandrasekar ,&nbsp;Jeevitha Vinayagam ,&nbsp;Kumar Chandrasekaran ,&nbsp;Thirugnanasambandam Rajendran ,&nbsp;S.A.S. Suriyaraj ,&nbsp;K.R. Arjun Aditiya","doi":"10.1016/j.algal.2025.104024","DOIUrl":"10.1016/j.algal.2025.104024","url":null,"abstract":"<div><div>Diatoms play a significant role in biofilm formation due to their unique surface properties and robust extracellular polymeric substance (EPS) production. This study investigates the influence of substrate and diatom surface physiochemical parameters on adhesion and biofilm productivity. Using <em>Amphora coffeiformis</em> COR_3 and <em>Nitschzia microcephala</em> COR_2 as a model organism, results highlighted that the adhesion density varied significantly with substratum type. The strongly polar <em>N. microcephala</em> COR_2 cells (γ^p = 32.5 ± 0.89 mJ/m²) favored increased adhesion towards glass with adhesion density of 3.017 × 10⁵ cells/mm². Conversely, <em>A. coffeiformis</em> COR_3 with its intense dispersive component (γ^LW = 24.1 ± 0.08 mJ/m²) promoted strong adhesion towards PE with adhesion density of 2.938 × 10⁵ cells/mm². <em>A. coffeiformis</em> favored adhesion with hydrophobic substrates via van der Waals interactions, facilitated by hydrophobic methylene groups on its surface. While, <em>N. microcephala</em> promoted adhesion with the hydrophilic substrates through acid-base interactions and hydrogen bonding. These findings are further complemented by the varying EPS composition with <em>A. coffeiformis</em> producing protein-rich EPS on hydrophobic substrates, while <em>N. microcephala</em> releasing carbohydrate-rich EPS on hydrophilic substrates. This surface chemistry driven biofilm formation provides a baseline for optimizing the diatom-substrate pairs to enhance biofilm performance in industrial applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104024"},"PeriodicalIF":4.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay between Ca2+ and ROS in regulating β-carotene biosynthesis in Dunaliella salina under high temperature stress","authors":"Jianxin Tang,&nbsp;Zizhou Zhang,&nbsp;Jinghan Wang,&nbsp;Fantao Kong,&nbsp;Zhanyou Chi","doi":"10.1016/j.algal.2025.104023","DOIUrl":"10.1016/j.algal.2025.104023","url":null,"abstract":"<div><div>Temperature influences biomass and β-carotene production in <em>Dunaliella salina</em>, while high temperature generally induces β-carotene biosynthesis via increased ROS, the intricate and incomplete mechanisms necessitate the identification of novel regulators and their interactions with ROS. A comprehensive analysis of physiological responses to different temperatures was investigated in this study, including cell proliferation, biomass growth, carbon utilization efficiency, β-carotene synthesis, chlorophyll profiles, photosynthetic efficiency, ROS, antioxidant content, activity of antioxidant enzymes, free radical scavenger capacity, and Ca²⁺ level. The results showed that 34 °C is the optimal temperature for β-carotene accumulation, and high temperature impairs chlorophyll, photosynthesis, and carbon utilization by enhancing oxidative stress. High temperature (40 °C vs 25 °C) triggers a simultaneous increase in ROS (24.34-fold) and Ca²⁺ levels (16.04-fold), implicating Ca²⁺ as a temperature-sensitive regulator of β-carotene that potentially interacts with ROS. Further chemical treatments at 34 °C demonstrated that Ca²⁺ supplementation enhanced biomass and increased β-carotene yield and content by 4.81 %, 11.79 %, and 19.99 %, respectively. Supplementation with H₂O₂ increased the yield and content of β-carotene (5.62 % and 30.57 %) but reduced biomass (19.23 %). Notably, supplemented H₂O₂ decreased cellular Ca²⁺, while supplemented Ca²⁺ enhanced antioxidant capacity. This study identifies Ca²⁺ as a novel regulator of high-temperature β-carotene biosynthesis and shows its interplay with ROS.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104023"},"PeriodicalIF":4.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of a novel polysaccharide from Tribonema minus and preparation of polysaccharide–selenium nanoparticles with enhanced antioxidant activity","authors":"Rundong Yang ,&nbsp;Yuhao Guo ,&nbsp;Yanchao Mao ,&nbsp;Wenxiang Zhang ,&nbsp;Feifei Wang ,&nbsp;Zhenzhou Zhu","doi":"10.1016/j.algal.2025.104020","DOIUrl":"10.1016/j.algal.2025.104020","url":null,"abstract":"<div><div><em>Tribonema</em> polysaccharide (TP), a biologically active polysaccharide containing β-1,3-glycosidic bonds, has not been extensively studied as a dispersing agent for stabilising selenium nanoparticles (SeNPs), and its synergistic effects with SeNPs remain poorly understood. This study investigates the relationship between the antioxidant activity and physicochemical properties of TP–SeNPs, which were synthesised through chemical reduction at varying TP-to‑selenium mass ratios (TPSMRs). TP was isolated from <em>Tribonema minus</em> using ultrasonic-assisted extraction, achieving a 119.64 % increase in yield under optimal conditions (400 W, 25 min, 1:400 solid-to-liquid ratio). Structural analysis identified TP as a novel β-1,3-glucan (8.46 kDa) composed exclusively of glucose units. Moreover, TP–SeNPs prepared at varying TPSMRs exhibited significantly higher antioxidant activity than either TP or SeNPs alone, demonstrating a clear synergistic effect. Notably, TP–SeNPs prepared at a TPSMR of 2:3 exhibited the lowest half-maximal inhibitory concentrations for scavenging 2,2-diphenyl-1-picrylhydrazyl, superoxide anions, and hydroxyl radicals (0.30, 0.77 and 0.46 mg/mL, respectively). This was attributed to the increased selenium content and reduced particle size of these complexes. Additionally, TP–SeNPs displayed excellent dispersibility and stability, likely due to non-covalent interactions between TP and SeNPs. In cellular assays, TP–SeNPs exhibited low cytotoxicity and dose-dependently enhanced cell viability under H₂O₂-induced oxidative stress. Cells treated with 200 mg/L TP–SeNPs exhibited a 35.83 % increase in viability compared with the model group. This protection was associated with elevated antioxidant enzyme activity, increased glutathione levels and reduced malondialdehyde content, thereby mitigating oxidative damage in HepG2 cells. This study is the first to characterise a novel β-1,3-glucan from <em>T. minus</em> and to elucidate the structure–activity relationships of TP–SeNPs, highlighting their potential as functional additives or pharmaceutical supplements.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104020"},"PeriodicalIF":4.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription factor MYB15 mediates docosahexaenoic acid biosynthesis and salt tolerance in Schizochytrium sp.","authors":"Yana Liu,&nbsp;Xiao Han,&nbsp;Pengyu Pan,&nbsp;Zhi Chen","doi":"10.1016/j.algal.2025.104016","DOIUrl":"10.1016/j.algal.2025.104016","url":null,"abstract":"<div><div><em>Schizochytrium</em> sp., a unicellular heterotrophic marine protist belonging to the thraustochytrids, serves as a commercial source of docosahexaenoic acid (DHA). Enhancing strain tolerance to environmental stress promotes strain robustness and productivity. Currently, we know very little about the transcription regulators that regulate DHA biosynthesis and abiotic stress response in thraustochytrids, which hinders strain improvement by transcription factor engineering strategies. In this study, we identified a 3R-MYB transcription factor, MYB15, which was transcriptionally downregulated under low-nitrogen condition in <em>Schizochytrium</em> sp. ATCC20888. Deletion of the <em>myb15</em> gene increased production of total lipids and DHA by 27.1% and 46.7%, respectively. Under high salt condition, <em>myb15</em>-deletion mutant ΔMYB15 displayed stronger salt tolerance and higher biomass than parental strain or complementation strain CMYB15. Electrophoretic mobility shift assays (EMSAs), DNase I footprinting assays, and transcriptome analysis demonstrated that MYB15 bound specifically to the 6-bp element 5′-CNGTTR-3′ in upstream regions of target genes, represses transcription of fatty acid biosynthetic genes (<em>dga1</em>, <em>fabD</em>, <em>fas</em>, and <em>pfa</em>) and salt tolerance-related genes (<em>sos2</em>, <em>sos3</em>, and <em>betA</em>). Taken together, we demonstrated that MYB15 acts as a critical repressor for DHA biosynthesis and salt stress response. Deletion of <em>myb15</em> generates a mutant with remarkable capabilities of DHA production and salt resistance, which make it a promising <em>Schizochytrium</em> strain for industrial application.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104016"},"PeriodicalIF":4.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental and model-based parameterization of the fundamental process kinetics of Chromochloris zofingiensis","authors":"Yob Ihadjadene ,&nbsp;Lory Ascoli ,&nbsp;Tehreem Syed ,&nbsp;Leon Urbas ,&nbsp;Thomas Walther ,&nbsp;Gunnar Mühlstädt ,&nbsp;Stefan Streif ,&nbsp;Felix Krujatz","doi":"10.1016/j.algal.2025.104012","DOIUrl":"10.1016/j.algal.2025.104012","url":null,"abstract":"<div><div>The microalgae <em>Chromochloris zofingiensis</em> has attracted considerable interest due to its remarkable ability to accumulate high value metabolites. Current research focuses primarily on laboratory-scale cultivation, while the development of fundamental model frameworks for process upscaling is neglected. In order to develop an effective scale-up process for <em>C. zofingiensis</em>, it is crucial to identify the strain-specific kinetics and dependencies on the environmental factors affecting its growth (light, temperature, pH, nutrients and dissolved oxygen concentration), as many of them remain unknown. This study represents a combination of different experimental and model-based approaches to parametrize the key growth parameters of <em>C. zofingiensis</em>, as well as its response kinetics towards different cultivation factors for model framework construction and calibration. The optimal temperature, DOC and PAR intensity for photosynthesis were successfully determined to be 26.58 °C, 6–7 mg_O₂/L, and 22.42 μmol/(m² s), respectively. This enabled the development of a model-based control strategy, by maintaining the average PAR intensity within the PBR at its optimal range of 20–30 μmol/(m² s), to ensure an efficient scale-up process, which effectively improved the productivity by a factor of 5 when scaling up the cultivation of <em>C. zofingiensis</em> to a 200 L pilot-scale PBR.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104012"},"PeriodicalIF":4.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfiltration of microalgae biomolecules: Impact of pulsed electric fields and high-pressure homogenization on single-cell protein recovery and membrane fouling","authors":"Byron Perez ,&nbsp;Zhao Li ,&nbsp;Iris Haberkorn ,&nbsp;Joseph Dumpler ,&nbsp;Alexander Mathys ,&nbsp;Peter Desmond","doi":"10.1016/j.algal.2025.104015","DOIUrl":"10.1016/j.algal.2025.104015","url":null,"abstract":"<div><div>This study investigated the separation of single-cell proteins from disrupted <em>Auxenochlorella protothecoides</em> microalgae using microfiltration. The microalgae were heterotrophically cultivated and subjected to two cell disruption methods, high-pressure homogenization (HPH) and pulsed electric field with incubation (PEF + inc), and stored at −20 °C. The impact of HPH and PEF + inc on the microfiltration performance of thawed samples was assessed. HPH treatment resulted in complete cell lysis, releasing a heterogeneous mixture of intracellular compounds. This, potentially combined with freezing effects, led to rapid membrane fouling and a 70 % decline in the permeate flux, finally stabilizing at 15 L m⁻² h⁻¹. In contrast, PEF + inc preserved cell integrity and particle size even after freezing, liberating mainly soluble compounds. This resulted in a more porous cake layer and a limited decline in the permeate flux, which stabilized at 24 L m⁻² h⁻¹. Additionally, higher protein transmission rates were achieved with PEF + inc (141.75 g m⁻² h⁻¹) than with HPH (54.53 g m⁻² h⁻¹) and it was 5.6 times more energy efficient. The cake layer formed by the HPH-treated samples had a higher resistance owing to smaller apparent pores and increased interaction with permeating proteins, which hindered protein transmission. These findings demonstrate that cell disruption methods can significantly influence downstream protein recovery efficiency and energy consumption. PEF + inc shows great potential as an energy-efficient bioprocessing method for microalgae biorefineries.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104015"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}