Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Key nutrients and their transportation processes influencing algal growth in a dam-controlled river","authors":"Jing Liu ,&nbsp;Qiuyu Liu ,&nbsp;Huaibin Wei ,&nbsp;Xinyu Zhang ,&nbsp;Wenhan Wan ,&nbsp;Yanmei Wang","doi":"10.1016/j.algal.2025.104017","DOIUrl":"10.1016/j.algal.2025.104017","url":null,"abstract":"<div><div>A dam-controlled river refers to a river where flow and water levels are regulated through hydraulic engineering structures, such as dams and sluices. This research focuses on controlling eutrophication in dam-controlled rivers, with particular attention to the Huaidian Dam on China's Shaying River. The primary objective of this research was to identify the key nutrients and their transportation processes affecting algal growth during the non-flood season. To achieve this, we analyzed algal density, species diversity, and the distribution of key nutrients, including ammonia nitrogen (NH₄⁺-N), nitrate nitrogen (NO₃⁻-N), and total phosphorus (TP) across dissolved, suspended, and sediment phases in both the hyporheic zone and surface water. The results reveal that diatoms dominate the algal community, comprising 66.91 % of the total composition and displaying higher cell density and biomass. Nutrient concentrations were mainly observed downstream of the dam, with NH₄⁺-N predominantly detected in sediments (2.92–9.23 mg/kg) and pore water (1.1–13.5 mg/L), NO₃⁻-N in sediment (2.5–12 mg/kg), and TP mainly in sediments (50–70 mg/kg). Redundancy analysis demonstrated that NO₃⁻-N in overlying water, sediment, and pore water were critical for algal growth, contributing 78.9 %, 77.5 %, and 75 %, respectively. Our findings suggest that the interaction between NO₃⁻-N transport and transformation within sediments and pore water is a significant factor influencing algal growth during non-flood seasons. This study highlights the influence of nutrient transport and transformation at the water-sediment interface for algal growth and recommends targeted measures to mitigate eutrophication risks in dam-controlled rivers.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104017"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel culture method with filter paper for isolating aerial microalgae under aerial-phase conditions","authors":"Nobuhiro Aburai,&nbsp;Yudai Ishikura,&nbsp;Katsuhiko Fujii","doi":"10.1016/j.algal.2025.104019","DOIUrl":"10.1016/j.algal.2025.104019","url":null,"abstract":"<div><div>Terrestrial microbial mats contain a wide variety of aerial microalgae, including useful drug-resistant and non-drug-resistant algal strains. A method for cultivating aerial microalgae in diverse terrestrial environments is needed to isolate these useful strains. The aim of this study was to develop a novel method for isolating aerial microalgae that does not rely on conventional antibiotics and characterize the isolated strains by screening and species identification. The effectiveness of filter paper as a culture substrate was investigated under aerial conditions; a total of 79 strains of aerial microalgae were successfully isolated from the microbial mat. After screening strains that grew well by altering the nitrogen source, pH, cultivation temperature, and light intensity of the medium, nine promising strains were identified from the isolated strains. Molecular phylogenetic trees suggested that algal strains were aerial microalgae of the genera <em>Coelastrella</em>, <em>Klebsormidium</em>, <em>Tetratostichococcus</em>, and <em>Chloroidium</em>. This simple, filter paper-based isolation method is thus effective for isolating aerial microalgae of various genera.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104019"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Illuminating microalgal biofilms using advanced photonic imaging techniques","authors":"Julia Mougin ,&nbsp;Anne-Sophie Pavaux ,&nbsp;Julien Deschamps ,&nbsp;Vlad Costache ,&nbsp;Filipa Lopes ,&nbsp;Romain Briandet","doi":"10.1016/j.algal.2025.104018","DOIUrl":"10.1016/j.algal.2025.104018","url":null,"abstract":"<div><div>In recent years, microalgal biofilms have garnered significant interest primarily due to their potential to transform wastewater treatment and overcome the limitations associated with conventional microalgal planktonic-based cultivation methods. Advances in imaging technologies have provided insight into the complex dynamics of microalgal biofilms, which could help in understanding both biofilm structure and function, and thus to optimize microalgae biofilm-based technologies. In particular, photonic microscopy allows researchers to study the architectural nuances, physiological responses, as well as spatial organization and interactions within biofilm communities. Despite these significant advances, there is still a lack of consistency in approaches and analytical techniques, which hinders standardized studies. Therefore, there is a need for common protocols and benchmarks in photosynthetic biofilm imaging. This review focuses primarily on key photonic imaging technologies, including confocal laser scanning microscopy (CLSM), light-sheet microscopy, histology coupled with a digital slide scanner, and optical coherence tomography (OCT). Principles, advantages/limitations and current applications of each technique in the context of microalgal biofilm studies, are presented allowing researchers to make reasoned decisions for their specific investigative endeavors.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104018"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colony formation of toxic Microcystis aeruginosa affected by microplastics-derived organic matter","authors":"Cunhao Du ,&nbsp;Wenlu Sang ,&nbsp;Yushen Ma ,&nbsp;Hui Chen ,&nbsp;Xiaoming Zhu ,&nbsp;Lixiao Ni ,&nbsp;Chu Xu ,&nbsp;Jiahui Shi ,&nbsp;Muyu Feng ,&nbsp;Mohamed Abbas ,&nbsp;Yiping Li ,&nbsp;Shiyin Li","doi":"10.1016/j.algal.2025.103997","DOIUrl":"10.1016/j.algal.2025.103997","url":null,"abstract":"<div><div>Cyanobacterial blooms and microplastic (MP) pollution have become increasingly prominent global environmental issues. This study investigated the effects of polyethylene-derived organic matter (PE-DOM) and polylactic acid-derived organic matter (PLA-DOM) on the growth and physiological characteristics of <em>Microcystis aeruginosa</em> (<em>M. aeruginosa</em>). Fluorescence spectroscopy revealed that both PLA-DOM and PE-DOM contained tryptophan-like compounds that promoted the growth of <em>M. aeruginosa</em>, with PLA-DOM exhibiting higher concentrations of these compounds. Elemental analysis indicated that both MPs-DOM provided essential trace elements such as Mg, Na, Ca, and Zn, which are crucial for algal growth. Both MPs-DOM treatments increased cell biomass, chlorophyll-<em>a</em> content, polysaccharide and protein contents, and the activities of catalase (CAT) and superoxide dismutase (SOD), while also improving photosynthetic parameters. These results suggest that MPs-DOM foster <em>M. aeruginosa</em> growth by enhancing nutrient availability, photosynthetic efficiency, and stress tolerance. Overall, <em>M. aeruginosa</em> showed better growth at higher concentrations of DOM and better growth under exposure to PLA-DOM. Furthermore, MPs-DOM enhanced the expression of <em>mcy</em>B and <em>mcy</em>D, leading to the production and release of microcystin-LR (MC-LR), which promoted the formation of dominant <em>M. aeruginosa</em> populations. These findings provide new insights into the formation of cyanobacterial blooms and the ecological risks posed by MP pollution.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 103997"},"PeriodicalIF":4.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae extracts reduce appetite in zebrafish larvae linked to long-chain fatty acids and 5′-methylthioadenosine","authors":"Javier Sanz Moxó ,&nbsp;Ana Catarina Fonseca ,&nbsp;Gabriela Matos ,&nbsp;Jorge Saraiva ,&nbsp;Ricardo Pereira ,&nbsp;Cristina Rocha ,&nbsp;Vítor Vasconcelos ,&nbsp;Ralph Urbatzka","doi":"10.1016/j.algal.2025.104003","DOIUrl":"10.1016/j.algal.2025.104003","url":null,"abstract":"<div><div>Appetite modulation is a promising strategy for obesity treatment. The presented research screened 103 microalgae extracts from three strains of microalgae (<em>Chlorella</em> sp.<em>, Chlorococcum</em> sp. and <em>Nannochloropsis oceanica</em>) for appetite reducing activity, using high pressure processing and moderate electric fields. Two different feeding assays with zebrafish larvae were applied using either fluorescent labeled liposomes or paramecia. Two extracts of <em>Chlorococcum</em> sp., reduced food uptake for &gt;30 % in the liposomes assay, while three extracts of <em>Chlorella</em> sp. (grown under autotrophic and heterotrophic conditions) for &gt;40 % in the paramecia assay. Real-time mRNA expression revealed that autotrophic grown <em>Chlorella</em> sp. increased the anorexigenic signaling of <em>cartpt</em> and <em>pomc</em>. Metabolite profiling with LCMS/MS was performed to get first insights into responsible compounds for appetite signaling. <em>Chlorococcum</em> presented a glycerolipid cluster with 9 highly correlated nodes and 6 putative identifications, while <em>Chlorella</em> sp. (heterotrophic) had 11 highly correlated nodes with 3 putative identifications as nucleotides and nucleosides. Following, unsaturated fatty acids (oleic acid (OA), linoleic acid (LA), palmitoleic acid (PA)), as members of the glycerolipid cluster, and adenosine (A) and 5-methylthioadenosine (5′-MTA), as member of the nucleosides, were commercially acquired and tested in both appetite assays in zebrafish. All unsaturated fatty acids reduced food intake in both assays, while 5′-MTA only in the liposomes assay. Overall, the extraction conditions of microalgae triggered promising appetite reduction activity in <em>Chlorella</em> and <em>Chlorococcum</em>, which could be linked to unsaturated fatty acids and 5′-MTA.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104003"},"PeriodicalIF":4.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multispectral imaging techniques for evaluating physicochemical-, antioxidant, and sensory properties of Alaria esculenta and Saccharina latissima","authors":"Anna Þóra Hrólfsdóttir ,&nbsp;Andrea Rakel Sigurðardóttir ,&nbsp;Hildur Inga Sveinsdóttir ,&nbsp;Sigurjón Arason ,&nbsp;Nette Schultz ,&nbsp;Hafsteinn Einarsson ,&nbsp;María Gudjónsdóttir","doi":"10.1016/j.algal.2025.104009","DOIUrl":"10.1016/j.algal.2025.104009","url":null,"abstract":"<div><div>With the increased seaweed production worldwide, there is a need to focus on improved production practices to produce high-quality seaweed biomass, especially if the biomass is intended for high-quality products and human consumption. Multispectral imaging (MSI) is a novel technique used as a quality control tool within the food industry due to its rapid, real-time measurements. Therefore, the study aimed to assess the possibilities of using MSI as a quality control tool within seaweed cultivation to predict the physicochemical (including proximate composition, trace minerals, pH, and color), microbiological (total viable counts (TVC)), sensory, and antioxidant properties of <em>A. esculenta</em> and <em>S. latissima</em> throughout diverse processing and handling. The results showed differences in spectra between species, and species classification got 100 % accuracy when using a Support Vector Machine (SVM) with the spectral data. Furthermore, results indicated that the partial least square regression (PLSR) models developed with cross-validation of the MSI data effectively predicted multiple quality parameters, including pH (<span><math><msubsup><mi>R</mi><mi>CV</mi><mn>2</mn></msubsup></math></span> = 0.94, RMSECV = 0.278), carbohydrate content (<span><math><msubsup><mi>R</mi><mi>CV</mi><mn>2</mn></msubsup></math></span> = 0.89, RMSECV = 0.76), protein content (<span><math><msubsup><mi>R</mi><mi>CV</mi><mn>2</mn></msubsup></math></span> = 0.94, RMSECV = 0.12), ash content (<span><math><msubsup><mi>R</mi><mi>CV</mi><mn>2</mn></msubsup></math></span> = 0.80, RMSECV = 0.51), and phenolic content (<span><math><msubsup><mi>R</mi><mi>CV</mi><mn>2</mn></msubsup></math></span> = 0.992, RMSECV = 0.24). In addition, the results showed possibilities of using the technology to assess several sensory properties. In conclusion, the results show the potential of using the MSI technology as an effective quality control tool within the seaweed industry to simultaneously evaluate multiple physicochemical properties of brown seaweed biomass.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104009"},"PeriodicalIF":4.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-large adsorption capacity for tetracycline by defatted Schizochytrium limacinum residue biochar","authors":"Liqin Lin ,&nbsp;Linjuan Ning ,&nbsp;Shiping Chen ,&nbsp;Baobei Wang ,&nbsp;Huaqiang Zhuang ,&nbsp;Xiaolian Jing ,&nbsp;Qingbiao Li","doi":"10.1016/j.algal.2025.104011","DOIUrl":"10.1016/j.algal.2025.104011","url":null,"abstract":"<div><div>Tetracycline (TC) is the one of the largest used antibiotics and its resulting water environment pollution has received widespread attention. In this paper, biochar was prepared by using the defatted <em>Schizochytrium limacinum</em> (microalgae) residue as raw material and KHCO₃ as activator, and its adsorption properties for TC were investigated. The results showed that KHCO₃-activation biochar possessed the developed hierarchical porous structure. The specific surface area was up to 2295.26 m²·g⁻¹, which was 409.6-fold higher than the pristine biochar. When the mass ratio of the pristine biochar to KHCO₃ was 1:1, the as-prepared biochar displayed the best adsorption performance for TC. The pseudo-second-order reaction kinetics can better describe the kinetic experiment results. The maximum adsorption capacity was 1357.5 mg·g⁻¹, which was more favorable than other biochar derived from agricultural waste reported previously. The adsorption capacity was stable under different solution conditions, suggesting that the microalgae residue-based biochar has a promising application foreground in wastewater treatment.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104011"},"PeriodicalIF":4.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution patterns and benthic assemblages associated with Pyropia columbina, a harvested alga from the Atlantic Patagonian coast","authors":"Mario Rodolfo Robert ,&nbsp;María Soledad Zabala ,&nbsp;María M. Mendez","doi":"10.1016/j.algal.2025.104014","DOIUrl":"10.1016/j.algal.2025.104014","url":null,"abstract":"<div><div>The benthic communities on rocky shores are shaped by a combination of biological, physical, and anthropogenic stressors. On Atlantic Patagonia's intertidal rocky shores, the seaweed <em>Pyropia columbina</em> plays an important ecological role and contributes economically to local communities. Despite its dominance in the benthic assemblage and the longstanding harvesting tradition, no previous studies have described changes in algae cover or the environmental factors shaping these patterns. Moreover, basic characteristics associated with the species have not been described in detail yet. The main objective of this study was to describe the populations of <em>P. columbina</em> along the Patagonian coast. Specific goals included evaluating the spatial and temporal distribution, abundance patterns of <em>P. columbina</em> and its associated benthic assemblages, and identifying the influence of environmental factors on these patterns. Additionally, we detailed the settlement substrates and attachment strength across different sites. Sampling was conducted at five locations on Patagonian coast, during two seasons (autumn and spring) over two years at the mid-intertidal level. Our results showed a greater cover of <em>P. columbina</em> in spring, related to its annual life cycle. Multivariate analyses of primary cover revealed similar assemblage structures across most sites. The main factors influencing the structure of benthic assemblages were wave exposure, rugosity, slope, maximum wave height, and suspended matter. <em>Perumytilus purpuratus</em> was the dominant species at most sites and the primary settlement substrate for <em>P. columbina</em>. The attachment strength of the algae was higher in spring and at sites with greater wave exposure, varying across different substrates. This study offers the first comprehensive description of the distribution patterns of <em>P. columbina</em> and its associated benthic communities on the rocky shores of Patagonia, providing valuable insights for the ecosystem-based management of these coastal areas.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104014"},"PeriodicalIF":4.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinity-dependent effects of seawater acidification on growth, photosynthetic physiology and biochemistry of the invasive macroalga Codium fragile","authors":"Yaoyao Chu ,&nbsp;Yunyun Shi ,&nbsp;Yuning Xue ,&nbsp;Lianghua He ,&nbsp;Xiaodong Li ,&nbsp;Xiangzhu Shi ,&nbsp;Yinggu Kuang ,&nbsp;Xinhua Chen ,&nbsp;Chao Zhao","doi":"10.1016/j.algal.2025.104010","DOIUrl":"10.1016/j.algal.2025.104010","url":null,"abstract":"<div><div>Ocean acidification (OA) and seawater salinity are two major environmental factors that influence the growth and distribution of macroalgae in coastal ecosystems. To investigate the effects of OA and salinity on the invasive macroalga <em>Codium fragile</em>, the growth, Chlorophyll <em>a</em> fluorescence, and biochemical compositions (pigment and soluble carbohydrate contents, the superoxide dismutase (SOD) activity, and malondialdehyde (MDA) contents) were studied after exposure to two pCO₂ levels (400 ppmv, LC; and 1000 ppmv, HC) and four salinity regimes (high salinity, 40 psu; control salinity, 30 psu; medium salinity, 20 psu; low salinity, 10 psu). The results showed that, except for SOD activity at 20 psu, the growth, maximum and effective quantum yield of PSII, and maximum relative electron transport, pigment and soluble carbohydrate contents, SOD activity, and the MDA content were adversely impacted by both hypo- and hypersaline under LC conditions. Similarly, under HC conditions, the growth, photosynthetic physiology and biochemistry were negatively impacted by low salinity, while high salinity enhanced pigment contents and chlorophyll fluorescence parameters but inhibited SOD activity and MDA contents. Furthermore, higher pCO₂ significantly promoted growth, pigment contents, and photosynthetic performance at 20 and 40 psu, while it amplified the depression in growth at 10 psu. These findings suggest that OA may enhance the potential invasive ability and salinity tolerance of <em>C. fragile</em> under medium hyposaline and hypersaline conditions by alleviating the negative effects of salinity stress on growth, photosynthesis, and pigments synthesis. However, it may also synergistically reduce algal growth at further reduced salinity. These data collected herein are valuable for understanding <em>C. fragile</em> cultivation and predicting its future distribution in response to changing ocean conditions.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104010"},"PeriodicalIF":4.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth characteristics and nutritional quality of two typical microalgae: Interactive effects of temperature, light, and nitrate","authors":"Yuwei Zhou ,&nbsp;Lele Jiang ,&nbsp;Yuxi Weng ,&nbsp;Haiyang Zong ,&nbsp;Zhenzhen Li ,&nbsp;Juntian Xu ,&nbsp;Futian Li","doi":"10.1016/j.algal.2025.104004","DOIUrl":"10.1016/j.algal.2025.104004","url":null,"abstract":"<div><div>In the context of increasing demand for food products, microalgae garnered considerable interest due to their essential roles in supporting aquatic food webs and serving as bait in aquaculture. In this regard, it is crucial to clarify their optimal culturing conditions, including both single-factor and multiple-factor combinations. In this study, <em>Thalassiosira weissflogii</em> and <em>Pyramimonas</em> sp. were cultivated under five levels of temperature, light intensity, and nitrate to determine their optimum growth conditions. A full factorial experiment was then conducted, employing two levels for each factor (10 and 25 °C for temperature; 50 and 150 μmol photons m⁻² s⁻¹ for light intensity; 2 and 100 μmol L⁻¹ for nitrate concentration) to investigate the growth, photosynthesis, and amino acid and fatty acid compositions of the two species. <em>T. weissflogii</em> exhibited an optimal growth temperature 4 °C higher than that of <em>Pyramimonas</em> sp., while the latter performed better at low temperatures. The full factorial experiment revealed that temperature was the most significant factor influencing the growth of two species. The fatty acid composition of <em>T. weissflogii</em> was primarily affected by temperature changes, with an average 4 % higher percentage of polyunsaturated fatty acids observed at high temperature. Nitrate concentration emerged as the key factor influencing photosynthetic rates, as well as soluble protein and carbohydrate contents, while it was the only factor affecting amino acid profiles. Generally, nitrate limitation resulted in lower photosynthetic rates, higher soluble carbonate contents, while soluble protein content and amino acid composition varied depending on the species and culturing conditions. These findings provide valuable information and reference points for enhancing biomass accumulation and the production of active substances in microalgae cultivation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"88 ","pages":"Article 104004"},"PeriodicalIF":4.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}