Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Carbon footprint assessment of microalgal biomass production, hydrothermal liquefaction and refining to sustainable aviation fuel (SAF) in mainland Portugal","authors":"Renata Pires ,&nbsp;Tiago P. Silva ,&nbsp;Cláudia Ribeiro ,&nbsp;Luís Costa ,&nbsp;Cristina T. Matos ,&nbsp;Paula Costa ,&nbsp;Tiago F. Lopes ,&nbsp;Francisco Gírio ,&nbsp;Carla Silva","doi":"10.1016/j.algal.2024.103799","DOIUrl":"10.1016/j.algal.2024.103799","url":null,"abstract":"<div><div>Industrial liquid effluents (e.g., from fertilizer industry) and flue gas streams (e.g., CO₂-rich, from cement industry) arise as an opportunity for waste valorization. Microalgae are suitable biomass for assimilating both effluents at the cultivation stage. Under a biorefinery concept, given the urge for energy transition in the aviation sector, this research explores the transformation of a microalgae consortium grown at an industrial site in Portugal and its subsequent harvesting, hydrothermal liquefaction (HTL), and bio-oil refining. A life cycle assessment (LCA) approach is undertaken with two functional units (FU): 1 kg of microalgae dry-cell weight (dw) and 1 MJ of bio-jet fuel. The latter follows an attributional approach with energy allocation for comparison with the Carbon Offsetting and Reduction Scheme for International Aviation (CORSIA) guidelines. HTL is based on data from bench-scale experiments and literature, whereby the Petroleum Refinery Life Cycle Inventory Model (PRELIM) is used to mimic bio-oil refining. Following this approach, achieving Sustainable Aviation Fuel (SAF) compliance requires net-zero electricity (0 gCO₂eq/kWh), with an HTL bio-oil yield of 55.6 % dw (the maximum observed), a minimum refining bio-jet fuel yield of at least 16 %. Alternatively, an HTL bio-oil yield of 36.9 % dw (the median observed) with a refining efficiency of at least 24.3 %.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103799"},"PeriodicalIF":4.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic microalgal co-cultivation for treatment of municipal wastewater using a two-stage cultivation system and biomass valorization","authors":"Rahul Kumar Goswami ,&nbsp;Sanjeet Mehariya ,&nbsp;Pradeep Verma","doi":"10.1016/j.algal.2024.103800","DOIUrl":"10.1016/j.algal.2024.103800","url":null,"abstract":"<div><div>The present study aimed to develop different microalgal co-cultivation <em>Tetraselmis indica</em> BDUG001 (TS) and <em>Picochlorum</em> sp. (PC) for the treatment and recycling of raw municipal wastewater (MWW). The nutrient removal and biomolecules production, 75 % raw MWW + 25 % ASN-III, 50 % secondary treated municipal wastewater (STMWW) + 50 % ASN-III, and 50 % TS-treated MWW (TSTMWW) + 50 % ASN-III was used. Among them, co-cultivation 2TS:1PC and 2PC:1TS exhibited maximum nutrient removal efficiency (NRE) and biomass and biomolecules production in 75 % raw MWW + 25 % ASN-III, and 50 % TSTMWW + 50 % ASN-III, respectively. Based on the above outcomes, two-stage cultivation systems (TSCSs) were developed to maximize the NRE and biomolecules production using the micro vertical glass photobioreactor (MVG-PBR). 2TS:1PC and 2PC:1TS co-cultivation ratio was used for first and second stage of cultivation and noted as 2TS:1PC (S1) and 2PC:TS (S2), respectively. The co-cultivation ratios were compared with monoculture TS (S1) and PC (S2), and the cost analysis was performed to assess the viability of the process. The obtained results showed that during the first stage of TSCSs, co-cultivation 2TS:1PC (S1) and TS (S1) exhibited NRE of COD (95 and 91.42 %), TN (93.06 and 89.54 %) and TP (100 and 93.87 %), and generated dry biomass (3.11 and 2.75 g/L), lipid content (46 and 42.17 %) having FAME content (82.25 and 32.01 %), astaxanthin (1.12 and 0.93 mg/g DCW) and β-carotene (7.01 and 3.30 mg/g DCW), respectively. During the second stage of TSCSs, co-cultivation 2PC:1TS (S2) and PC (S2) exhibited NRE of COD (95.50 and 93.28 %), TN (98.97 and 94.38 %) and TP (100 and 94.50 %), and generated biomass (3.24 and 2.78 g/L), lipid content (48.50 and 40.70 %) having FAME content (86.78 and 44.20 %), astaxanthin (1.15 and 0.71 mg/g DCW) and β-carotene (5.14 and 5.33 mg/g DCW), respectively. The cost analysis suggested that co-cultivation-assisted TSSCSs generated more revenue compared to monoculture-based TSCSs.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103800"},"PeriodicalIF":4.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving carbon-reduced catalytic gasification of microalgae for biohydrogen production","authors":"Ozlem Akca ,&nbsp;Junhui Chen ,&nbsp;Leilei Dai ,&nbsp;Kirk Cobb ,&nbsp;Yanling Cheng ,&nbsp;Paul Chen ,&nbsp;Hanwu Lei ,&nbsp;Roger Ruan","doi":"10.1016/j.algal.2024.103797","DOIUrl":"10.1016/j.algal.2024.103797","url":null,"abstract":"<div><div>To realize efficient biohydrogen production from microalgal biomass, catalytic gasification under optimized conditions was employed in this study for enhanced biohydrogen yield. Initially, the biochemical characteristics of <em>C. vulgaris</em> cultivated under stress conditions was investigated and then correlated with syngas production through the principal component analysis (PCA) to explore their effects on biohydrogen production. Subsequently, CaO catalyst-mediated catalytic gasification was developed and applied to convert <em>C. vulgaris</em> biomass to renewable biohydrogen with reduced carbon emission. The central composite design (CCD) method and regression analysis were used to optimize two essential gasification parameters, i.e., temperature at 600, 750, and 900 °C and catalyst loading with 0, 20, and 100 wt%, and also to investigate their synergetic effects on algae-to-biohydrogen conversion. The results demonstrated that microalgal biochemical compositions exerted obvious effects on subsequent biohydrogen production, especially enhanced lipid composition promoted biohydrogen yield. Further parameter optimization, particularly catalyst loading, was able to significantly improve H₂ production while reducing CO₂ generation. This study provides valuable insights into carbon-reduced biohydrogen production from renewable microalgal biomass for future sustainability development.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103797"},"PeriodicalIF":4.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water treatment of recirculating aquaculture system (RAS) effluent water through microalgal biofilms","authors":"Hanna Böpple ,&nbsp;Noor L.E. Kymmell ,&nbsp;Petronella Margaretha Slegers ,&nbsp;Peter Breuhaus ,&nbsp;Dorinde M.M. Kleinegris","doi":"10.1016/j.algal.2024.103798","DOIUrl":"10.1016/j.algal.2024.103798","url":null,"abstract":"<div><div>This research studied the growth of microalgae (<em>Chlorella vulgaris</em> and <em>Phaeodactylum tricornutum</em>) on a biofilm reactor using effluent water from salmon production in a recirculating aquaculture system (RAS). RAS effluent water contains considerable amounts of nitrate and small amounts of phosphate, that stem from dissolved excess feed and fish faeces. In microalgae growth experiments, we tested a twin-layer biofilm reactor, which has one layer for substrate distribution (RAS effluent water-based medium) and adhered onto that, a carrier layer for biofilm cultivation. First, we tested five different carrier materials (newsprint, filter paper, polypropylene, viscose/polyester mix, viscose) to assess the microalgae's attachment ability of the material, where the viscose fibre material proved to be the most suitable. The biofilm reactor design had to be improved for saltwater suitability, as water evaporation caused changes in salinity and nutrient concentrations and ultimately led to the formation of salt crusts on the biofilm and clogging of the irrigation system. A dilution of the medium with osmosis water compensated the evaporation rate and a technical improvement of the irrigation system established stable cultivation conditions. The biofilm reactor was then tested for all three water types (<em>Chlorella</em> for freshwater, <em>Phaeodactylum</em> for brackish water and saltwater) that are discharged during a RAS production cycle for salmon. Microalgae paste was used for inoculation of the biofilm carrier material and after a short maturation phase the biofilm reactor was harvested every three days. This study demonstrated that a complete uptake of nitrate and phosphate from RAS effluent water through microalgae cultivation is possible, and the biofilm reactor is able to handle changes in nutrient concentrations and salinity. Biomass productivity for <em>Phaeodactylum</em> cultivated on brackish RAS medium was highest (15.28 g m⁻² d⁻¹), compared to saltwater RAS medium (4.35 g m⁻² d⁻¹) and <em>Chlorella</em> on freshwater RAS medium (4.25 g m⁻² d⁻¹).</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103798"},"PeriodicalIF":4.6,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triphasic hydrogel for cell co-culture in compartmentalized all-liquid micro-bioreactor","authors":"Yuwen Meng ,&nbsp;Gabriel Giannini Beillon ,&nbsp;Marina Lauby ,&nbsp;Ines Elharar ,&nbsp;Benoît Schoefs ,&nbsp;Justine Marchand ,&nbsp;Erwan Nicol","doi":"10.1016/j.algal.2024.103803","DOIUrl":"10.1016/j.algal.2024.103803","url":null,"abstract":"<div><div>Biotechnological processes aiming at producing bio-compounds from microalgae have not yet reached economic sustainability because of the high costs of downstream processing. To improve economic profitability, microalgae immobilization and co-culture with non-photosynthetic organisms exchanging nutrients and metabolites constitutes an advantageous alternative to bulk mono-cultivation because of the higher productivity. However, the equilibrium between a symbiotic and competitive relationship is delicate in bacteria-microalgae co-cultures. The challenge remains in immobilizing and co-culturing them in separate compartments, thereby preserving the advantages of co-culture while eliminating the potential for competition. To achieve this objective, we propose an innovative strategy based on the immobilization of microalgae and bacteria in microstructured hydrogels. A triphasic poly(ethylene oxide) (PEO)-based hydrogel containing dextran and gelatin phases was created and characterized by confocal microscopy and mechanical tests. Several physicochemical parameters were tested for obtaining a core-corona structure that could host separately microalgae and bacteria, thus allowing the co-culture of both microorganisms. The hydrogel was used as a micro-bioreactor allowing immobilized microalgae and bacteria to be co-cultured in separated compartments for at least 7 days and kept alive over 5 weeks despite the degradation of the gelatin phase. Microalgae and bacteria multiplied in their respective compartments until they filled all the available space. The fitness of the microalgae was affected by the immobilization before returning to normal performance. Physiological measurements have qualified a core-corona structure within hydrogels as a good environment for co-culture and immobilization of microalgae, thus validating the proof-of-concept and opening up possibilities for future applications in biotechnology such as the production of biomolecules.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103803"},"PeriodicalIF":4.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep sequencing analysis of chloroplast transcription and splicing in Euglena gracilis","authors":"Yali Zhao,&nbsp;Pingwei Gao,&nbsp;Luoxi Tang,&nbsp;Guihong Xiang,&nbsp;Chengfu Sun","doi":"10.1016/j.algal.2024.103804","DOIUrl":"10.1016/j.algal.2024.103804","url":null,"abstract":"<div><div>The transcription and splicing of the <em>Euglena gracilis</em> (<em>E. gracilis</em>) chloroplast have been studied only on the scale of individual genes, and a comprehensive genome-scale analysis is lacking. We addressed this question in this study using transcriptomic RNA sequencing (RNA-seq) technology. Upon mapping tens of millions of RNA-seq reads onto the <em>E. gracilis</em> chloroplast genome, the transcription of protein-coding genes, including monocistronic genes and genes in operons, was visualized, and the expression levels of these genes were quantified. The splicing of intron-containing genes in the <em>E. gracilis</em> chloroplast genome was also visualized and quantified, with introns, including some twintrons, and exons being clearly discerned. The correct coding sequences of <em>psbD</em>, <em>rpl23</em> and <em>rpl16</em> were deduced based on read coverage of the corresponding genes and confirmed experimentally. Additionally, a new group III intron was identified from the 5’ UTR of <em>petB</em>. This study updates our knowledge on the transcription and splicing of the <em>E. gracilis</em> chloroplast, and will be instrumental in the mechanistic exploration of gene transcription and intron splicing in the <em>E. gracilis</em> chloroplast.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103804"},"PeriodicalIF":4.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resource-efficiency of cyanobacterium production on Mars: Assessment and paths forward","authors":"Tiago P. Ramalho ,&nbsp;Vincent Baumgartner ,&nbsp;Nils Kunst ,&nbsp;David Rodrigues ,&nbsp;Emma Bohuon ,&nbsp;Basile Leroy ,&nbsp;Guillaume Pillot ,&nbsp;Christiane Heinicke ,&nbsp;Sven Kerzenmacher ,&nbsp;Marc Avila ,&nbsp;Cyprien Verseux","doi":"10.1016/j.algal.2024.103801","DOIUrl":"10.1016/j.algal.2024.103801","url":null,"abstract":"<div><div>Space agencies and private companies strive for a permanent human presence on the Moon and ultimately on Mars. Bioprocesses have been advocated as key enablers due to their ability to transform locally available resources into added-value materials. However, the resource-efficiency and scaling of space biosystems remain poorly understood, hindering quantitative estimates of their potential performance. We leveraged extensive cultivation experiments, where a cyanobacterium (<em>Anabaena</em> sp. PCC 7938) was subjected to conditions attainable on Mars, to develop a model that can estimate bioprocess productivity and resource-efficiency as a function of water, light, temperature, regolith minerals and perchlorates, and atmospheric carbon and nitrogen. We show that a breakeven can be reached within a few years. We discuss research lines to improve both resource-efficiency and the accuracy of the model, thereby reducing the need for costly tests in space and eventually leading to a biotechnology-supported, sustained human presence on Mars.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103801"},"PeriodicalIF":4.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of CRISPR/Cas9 and Cas12a for gene editing in Chlamydomonas reinhardtii","authors":"Aron Ferenczi,&nbsp;Matthew Fellbaum,&nbsp;Yen Peng Chew,&nbsp;Catherine Kidner,&nbsp;Attila Molnar","doi":"10.1016/j.algal.2024.103796","DOIUrl":"10.1016/j.algal.2024.103796","url":null,"abstract":"<div><div>CRISPR/Cas-based technologies have revolutionized biology, offering a wide range of gene editing and engineering applications due to their diverse enzyme characteristics. Among the CRISPR/Cas nucleases, Cas9, and more recently, Cas12a (formerly known as Cpf1), have been employed in various gene editing applications in many eukaryotes, including the model green alga <em>Chlamydomonas reinhardtii</em>. To provide a comprehensive picture of their applicability in single-strand templated DNA repair and gene editing, we first mapped their targeting space by analysing their corresponding PAM frequencies, and then compared Cas9 and Cas12a activities by targeting overlapping regions at three independent loci in the <em>Chlamydomonas</em> genome. We identified 8 and 32 times more target sites for Cas9 compared to Cas12a within promoter regions and coding sequences, respectively. We found that Cas9 and Cas12a RNPs- co-delivered with ssODN repair templates- induced similar levels of total editing, achieving as much as 20–30 % in all viably recovered cells. Importantly, the level of precision editing was slightly higher for Cas12a. In contrast, Cas9 alone was able to induce more edits at the FKB12 locus than its Cas12a counterpart, overall making Cas9 the preferable enzyme for genome engineering among the currently available nucleases in <em>C. reinhardtii</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103796"},"PeriodicalIF":4.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of temperature on the oxygen production capacity and growth of scenedesmus almeriensis","authors":"Cristina Cerdá-Moreno ,&nbsp;Silvia Villaró-Cos ,&nbsp;Javier Tripiana ,&nbsp;Santiago Triviño de las Heras ,&nbsp;Joan Tarragona ,&nbsp;Tomás Lafarga","doi":"10.1016/j.algal.2024.103795","DOIUrl":"10.1016/j.algal.2024.103795","url":null,"abstract":"<div><div>The optimal temperature and irradiance to maximise oxygen production were 39.3 °C and 512.5 μmol photons·m⁻²·s⁻¹, respectively. These values were obtained by photorespirometry, which is a quick method to measure the photosynthetic and respiration rates of microalgae at a laboratory scale. With these conditions, the global oxygen production rate of <em>S. almeriensis</em> was 246.23 mg_oxygen·g_biomass⁻¹·h⁻¹. When the culture temperature was controlled at 39.3 °C for 1 h per day, the daily oxygen production capacity of <em>S. almeriensis</em> increased from 3129.5 mg_oxygen·g_biomass⁻¹ to 3778.5 mg_oxygen·g_biomass⁻¹. However, keeping the temperature at 39.3 °C for a longer time period caused a damage to the photosynthetic apparatus. This was validated using laboratory-scale bubble columns. The damage was reversible when heating the cells for &lt;2 h, but keeping the temperature of the culture at 39.3 °C for 3 h led to an irreversible damage and a 6 % decrease in the photosynthetic performance. Controlling the overheating of microalgal cultures is crucial to maximise growth. In addition, the duration of the exposure to high temperatures should also be included into growth and taken in consideration.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103795"},"PeriodicalIF":4.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic transformation of the freshwater diatom Cyclotella meneghiniana via bacterial conjugation","authors":"Wenxiu Yin ,&nbsp;Yanyan Zhang ,&nbsp;Qin Huang ,&nbsp;Yufang Pan ,&nbsp;Hanhua Hu","doi":"10.1016/j.algal.2024.103794","DOIUrl":"10.1016/j.algal.2024.103794","url":null,"abstract":"<div><div>Diatoms, the most species-rich algae, produce the main primary productivity of marine and freshwater ecosystems. Though, genetic transformation has been established in a variety of marine diatoms, genetic modification of freshwater diatoms is still difficult to achieve. Centric diatom <em>Cyclotella</em> is a major genus of freshwater diatoms, and <em>C. meneghiniana</em> is the most well-known and intensively studied species in this genus. In this study, episomal plasmids for <em>C. meneghiniana</em> were constructed, and endogenous promoters of <em>fucoxanthin chlorophyll a/c-binding protein 3</em> gene (<em>Fcp3</em>) or <em>ribosomal protein L14</em> gene (<em>RL14</em>) were used to drive the expression of <em>blasticidin-S deaminase</em> gene (<em>bsr</em>), <em>enhanced green fluorescent protein</em> gene (<em>eGFP</em>) and <em>β-glucuronidase</em> gene (<em>GUS</em>). The plasmids were introduced into algal cells by bacterial conjugation, and transformants were obtained by screening on solid plates containing 0.2 μg mL⁻¹ blasticidin-S with the transformation efficiency of 9–58 transformants per 10⁶ cells. PCR analysis verified the transfer of the plasmid sequences in the cells, and the fluorescence detection and staining analysis demonstrated that eGFP and GUS proteins were expressed in the cytoplasm, indicating the successful and stable expression of exogenous genes in <em>C. meneghiniana</em> through bacterial conjugation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103794"},"PeriodicalIF":4.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}