天然深共溶溶剂提取小球藻多糖

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-08-01 DOI:10.1016/j.algal.2025.104260

Bingfeng Zhou , Jiaming Jiang , Yu Huo , Juying Lin , Huiqing Zhang , Changhua Shang

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引用次数: 0

摘要

传统的多糖提取方法存在多糖降解、时间过长、温度过高导致多糖结构和生物活性发生变化等缺点。与传统的萃取方法相比，绿色天然深共晶溶剂（NADES）在各行业中具有广阔的应用前景。虽然NADES已被广泛用于提取藻类中的多糖等物质，但从小球藻中提取多糖的研究尚不多见。本研究采用超声辅助NADESs法提取核核小球藻多糖。在10种NADESs、蒸馏水和50%乙醇中，选择提取小球藻多糖效率最高的DES 7进行进一步研究。通过单因素实验和Box-Behnken设计（BBD）优化提取条件，获得最佳提取条件和提取效率。为了进一步研究小球藻多糖（CHP）的抗氧化活性，测定了小球藻多糖（CHP）对DPPH/ABTS自由基的清除能力和铁还原能力。最适宜的提取液为DES 7（氯化胆碱乙醇），提取率为3.09%。经响应面法的BBD实验优化后，DES 7的最佳提取条件为料液比1:30，摩尔比1:5.878，含水量20%。在理想的提取条件下，多糖CHP的实际提取效率为4.256%（高于初始的3.09%），明显高于以往研究中对小球藻多糖的提取效率（5.32 mg/g DW和17.35 mg/g DW）。此外，与冷冻干燥等其他干燥技术相比，采用喷雾干燥法制备核核小球藻粉末具有较低的生产成本。CHP具有较强的DPPH和ABTS清除能力（分别为38.19%和86.32%）和铁还原能力（OD700 = 1.56）。本研究提出了一种绿色提取小球藻多糖的新方法，为小球藻多糖在食品、化妆品和制药等行业的应用提供了良好的基础。

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Extraction of Chlorella polysaccharide using natural deep eutectic solvent

The conventional extraction methods of polysaccharides have some disadvantages such as polysaccharide degradation, the changes in structure and biological activity of polysaccharides due to too long time and too high temperature. Compared with the conventional extraction methods, the green natural deep eutectic solvent (NADES) has a promising application in various industries. Although NADES has been widely used to extract many substances from algae including polysaccharides, polysaccharide extraction from Chlorella was rare. In this study, Chlorella pyrenoidosa polysaccharides were extracted by ultrasound-assisted NADESs. Among 10 NADESs, distilled water and 50 % ethanol, DES 7 with the highest extraction efficiency of Chlorella polysaccharides was selected for further research. Single factor experiment and Box–Behnken design (BBD) optimization for extraction conditions of DES 7 were performed to obtain the best extraction conditions and extraction efficiency. To further investigate antioxidant activities of Chlorella polysaccharide (CHP), DPPH/ABTS radical scavenging activities and iron reducing power of polysaccharide CHP were determined. The most appropriate NADES was DES 7 (choline chloride:ethanol) with extraction efficiency of 3.09 %. After the experimental optimization with BBD of response surface methodology, the optimal extraction conditions for DES 7 were solid-to-liquid ratio of 1:30, molar ratio of 1:5.878 and water content of 20 %. Under the ideal extraction conditions, the real extraction efficiency of polysaccharides CHP was 4.256 % (more than the initial 3.09 %), which was obviously higher than that of polysaccharides from Chlorella vulgaris in previous studies (5.32 mg/g DW and 17.35 mg/g DW). Furthermore, Chlorella pyrenoidosa powder was prepared by spray drying with lower production cost compared with other drying techniques like freeze-drying. In addition, CHP exhibited strong DPPH and ABTS scavenging activities (38.19 % and 86.32 %) and iron reducing power (OD₇₀₀ = 1.56), respectively. In this study, a novel and green method for extracting Chlorella polysaccharide CHP was proposed, which provided the good basis for the application of Chlorella polysaccharides in food, cosmetic and pharmaceutical industries.

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment