IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-18 DOI: 10.1002/cbic.202580801

Jöri E. Wehrmüller, Julia C. Frei, Torsten Hechler, Michael Kulke, Andreas Pahl, Martin Béhé, Roger Schibli, Philipp R. Spycher

引用次数: 0

Front Cover: The Biarylitides: Understanding the Structure and Biosynthesis of a Fascinating Class of Cytochrome P450 Modified RiPP Natural Products (ChemBioChem 7/2025) 封面：The biarylides：了解一类迷人的细胞色素P450修饰的RiPP天然产物的结构和生物合成（ChemBioChem 7/2025）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-16 DOI: 10.1002/cbic.202580701

Leo Padva, Jemma Gullick, Laura J. Coe, Mathias H. Hansen, James J. De Voss, Max Crüsemann, Max J. Cryle

引用次数: 0

Cover Feature: Boronic Acid-Linked Apo-Zinc Finger Protein for Ubiquitin Delivery in Live Cells (ChemBioChem 7/2025) 封面专题：用于活细胞中泛素传递的硼酸连接载脂蛋白锌指蛋白（ChemBioChem 7/2025）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-16 DOI: 10.1002/cbic.202580702

Dr. Pritam Ghosh, Prof. Dr. Oliver Seitz

引用次数: 0

Efficient synthesis of cleavable CPP-cargo conjugate via low-equivalent of CPP activated by 2,2'-dithiodipyridine. 利用2,2'-二硫代二吡啶活化的低当量CPP高效合成可切割CPP-货物偶联物。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-09 DOI: 10.1002/cbic.202500032

Pincheng Li, Xiaona Han, Beichen Wang, Yanyan Guo, Yu Wang, Yiming Li

引用次数: 0

An Indole Dearomatization Strategy for the Synthesis of Pseudo-Natural Products. 假天然产物合成中的吲哚脱芳化策略。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-09 DOI: 10.1002/cbic.202500182

Joseph G F Hoock, Annina Burhop, Luca C Greiner, Beate Schölermann, Celine Da Cruz Lopes Guita, Jie Liu, Sukdev Bag, Axel Pahl, Sonja Sievers, Rebecca Scheel, Carsten Strohmann, Slava Ziegler, Michael Grigalunas, Herbert Waldmann

{"title":"An Indole Dearomatization Strategy for the Synthesis of Pseudo-Natural Products.","authors":"Joseph G F Hoock, Annina Burhop, Luca C Greiner, Beate Schölermann, Celine Da Cruz Lopes Guita, Jie Liu, Sukdev Bag, Axel Pahl, Sonja Sievers, Rebecca Scheel, Carsten Strohmann, Slava Ziegler, Michael Grigalunas, Herbert Waldmann","doi":"10.1002/cbic.202500182","DOIUrl":"https://doi.org/10.1002/cbic.202500182","url":null,"abstract":"The indole moiety is a privileged fragment that frequently populates existing bioactive compound collections. We describe the development of an indole-dearomatization sequence and its application for library expansion of a collection of indole-containing pseudo-NPs. The resulting compounds are topologically distinct from the original compound class. Phenotyping by means of the cell painting assay initially indicated that the dearomatized compounds are morphologically different than the original pseudo-NP compound class and guiding NPs. However, analysis by means of a new sub-profile analysis of the same cell painting assay data indicated that similar morphologies persisted throughout the compound classes. Further biological studies supported the findings of the sub-profile analysis and highlights its potential to more effectively characterize novel compounds. The biological findings suggest that a plethora of indole-dearomatization reactions could be applied to existing indole-containing compound collections to rapidly access new biologically relevant scaffolds.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500182"},"PeriodicalIF":2.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal Folding of Peptides into Single α-Helical Turns. 肽热折叠成单个α-螺旋匝。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-08 DOI: 10.1002/cbic.202500050

Erode N Prabhakaran, Ankur Kumar

引用次数: 0

Biochemical Characterization of Multimodular Xylanolytic Carbohydrate Esterases from the Marine Bacterium Flavimarina sp. Hel_I_48. 海洋细菌Flavimarina sp.多模块木聚糖水解碳水化合物酯酶的生化特性

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-08 DOI: 10.1002/cbic.202500058

Michelle Teune, Thorben Döhler, Daniel Bartosik, Thomas Schweder, Uwe T Bornscheuer

{"title":"Biochemical Characterization of Multimodular Xylanolytic Carbohydrate Esterases from the Marine Bacterium Flavimarina sp. Hel_I_48.","authors":"Michelle Teune, Thorben Döhler, Daniel Bartosik, Thomas Schweder, Uwe T Bornscheuer","doi":"10.1002/cbic.202500058","DOIUrl":"https://doi.org/10.1002/cbic.202500058","url":null,"abstract":"Carbohydrate-active enzymes (CAZymes) are critical for sustainable biomass utilization due to their ability to degrade complex polysaccharides. Frequently, a multimodularity can be observed combining several CAZyme domains and activities in close proximity which can benefit this degradation process. In this study, three multimodular xylanolytic carbohydrate esterases (CEs), named Fl6, Fll1, and Fll4, originating from Flavimarina sp. Hel_I_48 that represent a novel arrangement of catalytic and/or binding domains, are investigated. While Fl6 acts as a glucuronyl esterase, it also contains a carbohydrate binding module which is normally associated with xylanase activity. Fll1 combines xylosidase with acetylxylan esterase (AXE) activity mediated by a CE3 domain. The third enzyme, Fll4, is the first enzyme that comprises three distinct CE domains and shows bifunctional activity as an AXE and a feruloyl esterase (FAE). Investigation of the single domains reveals that the CE6 domain of Fll4 mediates its AXE activity while one of the putative CE1 domains, CE1a, mediates the FAE activity. This investigation of multimodularity of marine CAZymes not only enhances our understanding of these enzymes but may provide a promising route toward more efficient algal biomass utilization for biotechnological applications.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500058"},"PeriodicalIF":2.6,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Molecular Toolbox for Linkage Type-Specific Analysis of Ubiquitin Signaling. 泛素信号连锁类型特异性分析的分子工具箱。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-07 DOI: 10.1002/cbic.202500114

Julian Koch, Camilla Reiter Elbæk, Dominik Priesmann, Rune Busk Damgaard

{"title":"The Molecular Toolbox for Linkage Type-Specific Analysis of Ubiquitin Signaling.","authors":"Julian Koch, Camilla Reiter Elbæk, Dominik Priesmann, Rune Busk Damgaard","doi":"10.1002/cbic.202500114","DOIUrl":"https://doi.org/10.1002/cbic.202500114","url":null,"abstract":"Modification of proteins and other biomolecules with ubiquitin regulates virtually all aspects of eukaryotic cell biology. Ubiquitin can be attached to substrates as a monomer or as an array of polyubiquitin chains with defined linkages between the ubiquitin moieties. Each ubiquitin linkage type adopts a distinct structure, enabling the individual linkage types to mediate specific functions or outcomes in the cell. The dynamics, heterogeneity, and in some cases low abundance, makes analysis of linkage type-specific ubiquitin signaling a challenging and complex task. Here we review the strategies and molecular tools available for enrichment, detection, and characterization of linkage type-specific ubiquitin signaling. The molecular 'toolbox' consists of a range of molecularly different affinity reagents, including antibodies and antibody-like molecules, affimers, engineered ubiquitin-binding domains, catalytically inactive deubiquitinases, and macrocyclic peptides, each with their unique characteristics and binding modes. The molecular engineering of these ubiquitin-binding molecues makes them useful tools and reagents that can be coupled to a range of analytical methods, such as immunoblotting, fluorescence microscopy, mass spectrometry-based proteomics, or enzymatic analyses to aid in deciphering the ever-expanding complexity of ubiquitin modifications.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500114"},"PeriodicalIF":2.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Persistent Myth of Limonene's Smell. 柠檬烯气味的持久神话。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-07 DOI: 10.1002/cbic.202401085

Klaus Roth, Hans Bauer, Birte J Sjursnes, Lise Kvittingen, Rudolf Schmid

引用次数: 0

Synthesis and Structure of Naphthoyl Thiourea-Based Binuclear Ruthenium(II) Arene Complexes: Studies on Anticancer Activity and Apoptotic Mechanism. 萘基硫脲基双核钌（II）芳烃配合物的合成与结构：抗癌活性及凋亡机制研究。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-07 DOI: 10.1002/cbic.202500057

Abirami Arunachalam, Rengan Ramesh

{"title":"Synthesis and Structure of Naphthoyl Thiourea-Based Binuclear Ruthenium(II) Arene Complexes: Studies on Anticancer Activity and Apoptotic Mechanism.","authors":"Abirami Arunachalam, Rengan Ramesh","doi":"10.1002/cbic.202500057","DOIUrl":"https://doi.org/10.1002/cbic.202500057","url":null,"abstract":"Herein, we describe the synthesis, characterization and anticancer activity of ruthenium(II) p-cymene complexes comprising naphthoyl thiourea-based ligands. The synthesized ruthenium(II) complexes(1-3) were fully characterized by elemental analysis and spectral methods. The structure of complex 2 has been elucidated by employing single crystal X-ray diffraction (SC-XRD), which verifies the two bidentate N^O and N^S coordination of the thiourea ligand to two Ru(II) centres. Further, the complexes have been subjected to stability studies to illustrate their aquation behavior in an aqueous medium. All the complexes have been screened for their anticancer efficacy in Breast (MCF-7), Colon (HT-29), Liver (HepG2) cancerous cells and non-cancerous kidney (Hek-293) cells. Among them, complex 2 with an IC50 concentration of 3.59 ± 0.72 µM exhibits the most potent activity in HT-29 cells, surpassing the positive control, cisplatin. In addition, AO-EB and Hoechst labelling of all the complexes(1-3) on HT-29 cells reveals morphological alterations such as nuclear fragmentation and chromatin condensation resulting from the death of cancerous cells via apoptosis. Biochemical assays such as reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and flow cytometry strongly confirm the cell death via mitochondrial dysfunction-mediated apoptosis.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500057"},"PeriodicalIF":2.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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