IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-04 DOI: 10.1002/cbic.202500122

Mussarat Tasleem, Asaad Mohamad Matouk, Manzar Abbas

{"title":"Design of Short Peptides for the Reduction of Silver Ions and Stabilization of Nanocomposites in Combating Bacterial Infections.","authors":"Mussarat Tasleem, Asaad Mohamad Matouk, Manzar Abbas","doi":"10.1002/cbic.202500122","DOIUrl":"https://doi.org/10.1002/cbic.202500122","url":null,"abstract":"Combating bacterial infections has become a formidable challenge in healthcare due to the rise of antibiotic resistance. Recently, short peptide-based nanobiomaterials, assembled through silver metal and peptide building blocks, have emerged as promising antibiotic agents for treating resistant bacterial infections. In this minireview, recent advances in silver-peptide nanocomposites are highlighted, both with and without the assistance of UV or sunlight, for antibacterial applications. The chemical design of biomolecules such as amphiphilic short peptides, amino acids, and oligopeptides plays a crucial role in the reduction, stabilization, and biocompatibility of silver-peptide nanocomposites. Noncovalent interactions involved in the formation of nanocomposites are explored in the context of the structure-function relationship. The antibacterial activities and underlying mechanisms, which depend on specific peptide building blocks, are also reviewed. Finally, the conclusion and the outlook provide insights into the design of novel peptide building blocks for the development of silver-peptide nanocomposites with enhanced antibacterial activities.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500122"},"PeriodicalIF":2.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boron-Based Functionalities Enhance, the Potency of 2,5-Dimethylfuran-Based IDO1 Inhibitors.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-04 DOI: 10.1002/cbic.202500134

Thomas J C Carraro, Samrat Dasgupta, Jacqueline Ku, Shane R Thomas, Louis M Rendina

引用次数: 0

Designing Enzymatic Reactivity with an Expanded Palette.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-04 DOI: 10.1002/cbic.202500076

Reuben B Leveson-Gower

{"title":"Designing Enzymatic Reactivity with an Expanded Palette.","authors":"Reuben B Leveson-Gower","doi":"10.1002/cbic.202500076","DOIUrl":"https://doi.org/10.1002/cbic.202500076","url":null,"abstract":"The expanding applications of biocatalysis in the chemical and pharmaceutical sectors herald a greener future for these industries. Yet, the range of chemical reactions known to enzymes only covers a small fraction of what is required for modern synthetic routes. To continue the increases in sustainability afforded by converting chemical processes into enzymatic ones, fundamentally new kinds of biocatalytic reactivity are required. Perhaps the very components from which enzymes are constructed, a palette of canonical amino acids and cofactors, inherently limit their catalytic possibilities, even if all the available natural sequence space can be explored. In recent years, there has been an explosion of strategies to produce new biocatalytic function through the incorporation of noncanonical amino acids and synthetic cofactors, new colors which are added to the enzyme design palette. This has enabled new enzymatic reactions that proceed via organocatalytic, organometallic, and photocatalytic mechanisms. Aside from designing new enzymatic activities from scratch, exogenous photocatalysts have recently also been used in synergy with natural enzyme active sites to diverge their reactivity towards radical pathways. This review will highlight recent developments in enriching enzymatic chemistry with new unnatural components, providing an outlook for future directions and needed developments for practicality and sustainability.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500076"},"PeriodicalIF":2.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Bacterial Sulfotransferase Catalyzes an Unusual Di-Sulfation in Natural Products Biosynthesis.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-04 DOI: 10.1002/cbic.202500024

Conor Pulliam, Lukuan Hou, Dan Xue, Mingming Xu, Katherine Holandez-Lopez, Jie Li

{"title":"A Bacterial Sulfotransferase Catalyzes an Unusual Di-Sulfation in Natural Products Biosynthesis.","authors":"Conor Pulliam, Lukuan Hou, Dan Xue, Mingming Xu, Katherine Holandez-Lopez, Jie Li","doi":"10.1002/cbic.202500024","DOIUrl":"https://doi.org/10.1002/cbic.202500024","url":null,"abstract":"Sulfation is a widely used strategy in nature to modify the solubility, polarity, and biological activities of molecules. The enzymes catalyzing sulfation, sulfotransferases (STs), are typically highly specific to a single sulfation site in a molecule. Herein, the identification and characterization of sulfated adipostatins is reported and reveals a novel sulfotransferase, AdpST, which is responsible for di-sulfation at two sites of adipostatins. The initial bioinformatic analysis in search of adipostatin analogs from Streptomyces davaonensis DSM101723 identifies adpST and a 3'-phosphoadenosine-5'-phosphosulfate (PAPS) biosynthetic cassette, which are co-clustered with the adipostatin-encoding type III polyketide synthase. Mono- and di-sulfated adipostatin analogs are discovered in the extracts of S. davaonensis DSM101723, whereas di-sulfated bacterial natural products has not been reported. Using a series of in vivo and in vitro experiments, it is confirmed that AdpST is solely responsible for both mono- and di-sulfation of adipostatins, a catalytic activity which has not been identified in bacterial PAPS-dependent STs to date. It is further demonstrated that the dedicated PAPS biosynthetic cassette improves di-sulfation capacity. Lastly, it is determined that AdpST shares similarity with a small group of uncharacterized STs, suggesting the presence of additional unique bacterial STs in nature, and that AdpST is phylogenetically distant from many characterized STs.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500024"},"PeriodicalIF":2.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrocycles of Saxitoxin: Insights into the Structure of Zetekitoxin AB.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-04 DOI: 10.1002/cbic.202500170

Wenyuan Li, Srinivas R Paladugu, Jordan P Liles, Manju Karthikeyan, Kevin Chase, Shrinivasan Raghuraman, Matthew S Sigman, Ryan E Looper

引用次数: 0

Bioorthogonal Post-Labeling Reveals Nuclear Localization of a Highly Cytotoxic Half-sandwich Ir(III) Tetrazine Complex in Live Cells.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-03 DOI: 10.1002/cbic.202500090

Alfonso Annunziata, Sadek Amhaz, Jérémy Forté, Geoffrey Gontard, Romain Morichon, Joëlle Sobczak-Thépot, Michele Salmain

{"title":"Bioorthogonal Post-Labeling Reveals Nuclear Localization of a Highly Cytotoxic Half-sandwich Ir(III) Tetrazine Complex in Live Cells.","authors":"Alfonso Annunziata, Sadek Amhaz, Jérémy Forté, Geoffrey Gontard, Romain Morichon, Joëlle Sobczak-Thépot, Michele Salmain","doi":"10.1002/cbic.202500090","DOIUrl":"https://doi.org/10.1002/cbic.202500090","url":null,"abstract":"Intracellular imaging of anticancer metallodrugs often relies on pre-labeling with organic fluorophores, which significantly affects their physicochemical properties and intracellular distribution. On the other hand, the reported post-labeling strategies based on click-chemistry reactions require cell fixation and permeabilization. Here, we present a post-labeling approach based on the catalyst-free, inverse electron-demand Diels-Alder reaction (iEDDA) between a strained fluorescein-tagged bicyclononyne derivative (BCN-FAM) and half-sandwich Ir(III) complexes containing bidentate ligands comprising a tetrazine (Tz-R,R') entity. Five half-sandwich Ir(III) complexes with formula [Cp*Ir(Tz-R,R')Cl]0/+ have been synthesized and fully characterized, including the X-ray crystal structures of three of the five derivatives. Investigations of their stability and their reactivity in aqueous solution and in a model iEDDA reaction revealed the strong influence of the tetrazine ligand structure on the chemical properties of the corresponding complexes. A highly cytotoxic metallodrug candidate (Ir-C,NPh,Me) was identified from biological studies, and chemical reactivity studies disclosed an unusual preference for binding of methionine over cysteine. Post-labeling of Ir-C,NPh,Me in live HeLa cells highlighted its preferential accumulation within the nucleus, suggesting its retention through covalent modifications of nuclear proteins in good agreement with other half-sandwich iridium(III) complexes.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500090"},"PeriodicalIF":2.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and Application of the Purified Cell-free System.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-03 DOI: 10.1002/cbic.202500176

Chao Liao, Hengrun Li, Haotian Zheng, Chaofeng Li, Liangxu Liu, Jiawei Wang, Jun Ni

引用次数: 0

Induction and manipulation of biomolecular condensates through spatially heterogeneous solution conditions.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-03 DOI: 10.1002/cbic.202500044

Alexander Kai Buell

引用次数: 0

Phase separation in biochemical and biological systems: Implications for disease pathogenesis.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-03 DOI: 10.1002/cbic.202400883

Raj Dave, Kshipra Pandey, Ritu Patel, Raghu Solanki, Nidhi Gour, Dhiraj Bhatia

引用次数: 0

SAAP-148 oligomerizes into a hexamer forming a hydrophobic inner core.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-01 DOI: 10.1002/cbic.202500112

Nermina Malanovic, Aden Hodzic, Djenana Vejzovic, Altea Topciu, Kirill Kuhlmann, Raj Kumar, Maria Andrea Mroginski, Alejandra Andrea de Miguel, Pia Hofmann, Klaus Zangger, Markus Weingarth, Robert A Cordfunke, Jan W Drijfhout, Peter Nibbering, Michal Belicka, Karl Lohner

{"title":"SAAP-148 oligomerizes into a hexamer forming a hydrophobic inner core.","authors":"Nermina Malanovic, Aden Hodzic, Djenana Vejzovic, Altea Topciu, Kirill Kuhlmann, Raj Kumar, Maria Andrea Mroginski, Alejandra Andrea de Miguel, Pia Hofmann, Klaus Zangger, Markus Weingarth, Robert A Cordfunke, Jan W Drijfhout, Peter Nibbering, Michal Belicka, Karl Lohner","doi":"10.1002/cbic.202500112","DOIUrl":"https://doi.org/10.1002/cbic.202500112","url":null,"abstract":"Human cathelicidin LL-37 is a widely studied antimicrobial 37-mer peptide with various ascribed functions, that serves as a template for designing novel peptides. Its derivative, the 24-mer SAAP-148, is highly effective in vitro in eradicating multidrug-resistant bacteria, persistent cells, and biofilms, without inducing resistance. SAAP-148 is characterized by a high cationic charge (+11) and 46% hydrophobicity, which, once the peptide folds into an alpha helix, forms a wide hydrophobic face. This highly amphipathic nature facilitates on the one hand its insertion into the membrane's fatty acyl chain region and on the other hand it´s interaction with anionic membrane components, which contributes to its mode of action in killing bacteria. However, the contributions of the secondary and quaternary structures remain underexplored. To address this, we conducted a study using anionic and zwitterionic membrane models, applying circular dichroism, NMR spectroscopy, X-ray scattering, AlphaFold 3 and molecular dynamics simulations. Our results reveal that SAAP-148 adopts a stable hexameric bundle composed of three parallel dimers, creating a hydrophobic core of aromatic residues. This structure is retained at the membrane interface, where MD simulations suggest the formation of a fiber-like structure on anionic membranes, most likely driven by exposed cationic side chains.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500112"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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