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A Mn(salen)-Based Artificial Metalloenzyme for Nitrene and Oxene Transfer Catalysis.
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-12 DOI: 10.1002/cbic.202400774
Zhennan Liu, Yee-Song Law, Ravi Kumar Verma, Yi Ling Goh, Mun Fei Eddy Wong, Barindra Sana, Hao Fan, Ee Lui Ang, Yee Hwee Lim
{"title":"A Mn(salen)-Based Artificial Metalloenzyme for Nitrene and Oxene Transfer Catalysis.","authors":"Zhennan Liu, Yee-Song Law, Ravi Kumar Verma, Yi Ling Goh, Mun Fei Eddy Wong, Barindra Sana, Hao Fan, Ee Lui Ang, Yee Hwee Lim","doi":"10.1002/cbic.202400774","DOIUrl":"https://doi.org/10.1002/cbic.202400774","url":null,"abstract":"<p><p>The development of artificial metalloenzymes (ArMs) offers a potent approach to incorporate non-natural chemical reactions into biocatalysis. Here we report the assembly of Mn(salen)-based ArMs by embedding biotinylated Mn(salen) complexes into streptavidin (Sav) variants. Using commercially available nitrene and oxo transfer reagents, these biohybrid catalysts catalyzed the aziridination of alkenes and oxidation of benzylic C-H bonds with up to 19 and 146 turnover numbers.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400774"},"PeriodicalIF":2.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of linker length on the function of biotinylated OSW-1 probes.
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-12 DOI: 10.1002/cbic.202400923
Myat Nyein Khine, Naho Isogai, Tomoya Takeshita, Kaori Sakurai
{"title":"Effect of linker length on the function of biotinylated OSW-1 probes.","authors":"Myat Nyein Khine, Naho Isogai, Tomoya Takeshita, Kaori Sakurai","doi":"10.1002/cbic.202400923","DOIUrl":"https://doi.org/10.1002/cbic.202400923","url":null,"abstract":"<p><p>The biotinylated probes based on anticancer saponin OSW-1 with varied linker lengths were synthesized and their cell growth inhibitory activity and affinity pulldown efficiency were evaluated. All the probes possessed similar cytotoxicity compared to the parent natural product, showing that the linker moiety did not significantly affect cell uptake or target engagement. In contrast, when evaluated against the known target proteins, OSBP and ORP4, the biotinylated probe 3 with PEG5 linker enabled most effective enrichment of target proteins in an affinity pulldown assay, suggesting that the cytotoxicity and pulldown efficiency did not correlate among the probes studied. The selectivity of affinity pulldown using the optimal probe was also verified by facile identification of the enriched protein by silver staining and LC/MS analysis. Therefore, probe 3 with PEG5 linker comprising of 25 atoms (28 Å) was found to be an optimal biotinylated probe for isolating OSW-1 binding proteins from cell lysate.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400923"},"PeriodicalIF":2.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Position-Regulated Electrostatic Interactions for Single Amino Acid Revealed by Aspartic Acid-Scanning Mutagenesis.
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-12 DOI: 10.1002/cbic.202400891
Mengting Chen, Lilusi Ma, Minxian Li, Xiaocui Fang, Yanlian Yang, Chen Wang
{"title":"Position-Regulated Electrostatic Interactions for Single Amino Acid Revealed by Aspartic Acid-Scanning Mutagenesis.","authors":"Mengting Chen, Lilusi Ma, Minxian Li, Xiaocui Fang, Yanlian Yang, Chen Wang","doi":"10.1002/cbic.202400891","DOIUrl":"10.1002/cbic.202400891","url":null,"abstract":"<p><p>We have examined in this contribution the electrostatic interactions between single arginine and aspartic acid by analyzing the peptide-peptide binding characteristics involving arginine-aspartic acid, arginine-glycine, arginine-tryptophan and tryptophan-glycine interactions. The results of aspartic acid mutagenesis revealed that the interactions between arginine and aspartic acid have significant dependence on the position and composition of amino acids. While the primary interaction can be attributed to arginine-tryptophan contacts originated from the indole moieties with the main chains of 14-mers containing N-H and C=O moieties, pronounced enhancement could be identified in association with the electrostatic side-chain-side-chain interactions between arginine and aspartic acid. An optimal separation of 2~4 amino acids between two adjacent aspartic acid and tryptophan binding sites can be identified to achieve maximal enhancement of binding interactions. Such observed separation dependence may be utilized to unravel cooperative effects in heterogeneous interactions between single pair of amino acids.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400891"},"PeriodicalIF":2.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dinuclear Ru(II) Complexes Containing Tetrapyrido[3,2-a : 2,3-c : 3,2-h : 2''',3'''-j]Phenazine Ligand for Biomedical Applications.
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-11 DOI: 10.1002/cbic.202400931
Rajesh Kushwaha, Samya Banerjee
{"title":"Dinuclear Ru(II) Complexes Containing Tetrapyrido[3,2-a : 2,3-c : 3,2-h : 2''',3'''-j]Phenazine Ligand for Biomedical Applications.","authors":"Rajesh Kushwaha, Samya Banerjee","doi":"10.1002/cbic.202400931","DOIUrl":"10.1002/cbic.202400931","url":null,"abstract":"<p><p>Ruthenium complexes are among the most extensively studied and developed luminescent transition-metal complexes for anticancer applications. Dinuclear Ru(II) complexes have caught significant interest for larger size, higher charge, and variable complex shapes. In this concept, we have explored past and recent works on the possible biological applications of versatile tetrapyrido[3,2-a : 2,3-c : 3,2-h : 2''',3'''-j]phenazine (tppz)-based dinuclear Ru(II) complexes with a focus on their use as quadruplex DNA probes, organelle imaging, and phototherapeutic agents. This concept also points out that a particular type of dinuclear Ru(II) complexes can act as multitargeting and multifunctional anticancer agents -making this an exciting research area in which an array of further applications will likely emerge.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400931"},"PeriodicalIF":2.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CORRIGENDUM: Corrigendum: Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals rbbp4 as a Cellular Target of Protopanaxadiol Against Colorectal Cancer Cells [Chembiochem. 2022; 23(13): e202200038].
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-11 DOI: 10.1002/cbic.202400766
{"title":"CORRIGENDUM: Corrigendum: Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals rbbp4 as a Cellular Target of Protopanaxadiol Against Colorectal Cancer Cells [Chembiochem. 2022; 23(13): e202200038].","authors":"","doi":"10.1002/cbic.202400766","DOIUrl":"https://doi.org/10.1002/cbic.202400766","url":null,"abstract":"","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400766"},"PeriodicalIF":2.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Upgraded Solid-Phase Assembly of Chelators (DOTA and NOTA) Enabled Bacterial Uptake Studies of Radiolabeled Peptide.
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-10 DOI: 10.1002/cbic.202400996
Bibekananda Pati, Anuj Kumar, Arnab Chowdhury, Nitesh Mani Tripathi, Vinod Gour, Archana Mukherjee, Anupam Bandyopadhyay
{"title":"An Upgraded Solid-Phase Assembly of Chelators (DOTA and NOTA) Enabled Bacterial Uptake Studies of Radiolabeled Peptide.","authors":"Bibekananda Pati, Anuj Kumar, Arnab Chowdhury, Nitesh Mani Tripathi, Vinod Gour, Archana Mukherjee, Anupam Bandyopadhyay","doi":"10.1002/cbic.202400996","DOIUrl":"10.1002/cbic.202400996","url":null,"abstract":"<p><p>Among popular radio metal chelators, DOTA and NOTA have been remarkably considered in radionuclide therapy and imaging studies due to several advantages in pharmacology. Here, we developed a practical and general method for assembling DOTA and NOTA in the solid phase peptide (pseudo-dilute conditions) using a wide range of solvents with easily accessible and economical feedstocks, which mitigated unprecedented challenges associated with previously reported methods. This upgraded approach enabled an efficient installation of these two chelators on various bioactive peptide sequences. Finally, we assessed the antimicrobial activity of the DOTA- and NOTA-attached Combi peptides to B. subtilis, which was intact. The authenticity of the assembled DOTA framework was assessed by labeling <sup>177</sup>Lu and in vitro bacterial uptake in E. coli and S. aureus. <sup>177</sup>Lu-labeled DOTA-Combi peptide exhibited promising uptake for developing a bacterial infection imaging agent while negligible hemolysis activity even at >200 μM. This contribution will be valued for developing peptide radiopharmaceuticals with operational simplicity and economic approaches.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400996"},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing Nanomaterials for Enhanced DNA-Based Biosensing and Therapeutic Performance.
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-10 DOI: 10.1002/cbic.202400936
Xumin Pan, Xiaoman Zhao, Yanhong Lu, Ping Xie, Lan Liu, Xia Chu
{"title":"Harnessing Nanomaterials for Enhanced DNA-Based Biosensing and Therapeutic Performance.","authors":"Xumin Pan, Xiaoman Zhao, Yanhong Lu, Ping Xie, Lan Liu, Xia Chu","doi":"10.1002/cbic.202400936","DOIUrl":"10.1002/cbic.202400936","url":null,"abstract":"<p><p>The integration of nanomaterials with DNA-based systems has emerged as a transformative approach in biosensing and therapeutic applications. Unique features of DNA, like its programmability and specificity, complement the diverse functions of nanomaterials, leading to the creation of advanced systems for detecting biomarkers and delivering treatments. Here, we review the developments in DNA-nanomaterial conjugates, emphasizing their enhanced functionalities and potential across various biomedical applications. We first discuss the methodologies for synthesizing these conjugates, distinguishing between covalent and non-covalent interactions. We then categorize DNA-nanomaterials conjugates based on the properties of the DNA and nanomaterials involved, respectively. DNA probes are classified by their application into biosensing or therapeutic uses, and, several nanomaterials are highlighted by their recent progress in living biological. Finally, we discuss the current challenges and future prospects in this field, anticipating that significant progress in DNA-nanomaterial conjugates will greatly enhance precision medicine.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400936"},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quadruplex DNA Hybrid Catalysts for Enantioselective Reactions.
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-10 DOI: 10.1002/cbic.202400909
Zixiao Wang, Xingchen Dong, Yashao Chen, Changhao Wang
{"title":"Quadruplex DNA Hybrid Catalysts for Enantioselective Reactions.","authors":"Zixiao Wang, Xingchen Dong, Yashao Chen, Changhao Wang","doi":"10.1002/cbic.202400909","DOIUrl":"10.1002/cbic.202400909","url":null,"abstract":"<p><p>Beyond the pivotal genetic roles of DNA, its duplex structures as chiral scaffolds interacting with metal complexes give rise to DNA hybrid catalysts for a set of aqueous-phase enantioselective reactions. Besides DNA duplex, DNA quadruplexes including G-quadruplex and i-motif show tunable structures with variable non-canonical base pairs. In this concept, based on the interaction between metal species and DNA, we classify the construction strategies of quadruplex DNA hybrid catalysts into supramolecular, covalent and coordinative modes. Furthermore, we analyze the relationship between DNA quadruplexes and their enantioselective catalytic performance. Finally, we summarize the current challenges and look forward to the future directions in DNA-based enantioselective catalysis.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400909"},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research Progress in the Field of Hydrogen Sulfide Donors in the Last Five Years.
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-10 DOI: 10.1002/cbic.202400817
Qian Pang, Fangjun Huo, Caixia Yin
{"title":"Research Progress in the Field of Hydrogen Sulfide Donors in the Last Five Years.","authors":"Qian Pang, Fangjun Huo, Caixia Yin","doi":"10.1002/cbic.202400817","DOIUrl":"https://doi.org/10.1002/cbic.202400817","url":null,"abstract":"<p><p>Hydrogen sulfide (H<sub>2</sub>S) serves as the third gasotransmitter, crucial in various physiological processes involving its production and metabolism. Elevated levels of H<sub>2</sub>S can result in acute or chronic poisoning, whereas lower concentrations are involved in regulating diverse physiological and pathological activities within the human body. Moreover, it actively participates in maintaining normal cellular function by exerting cell protection and anti-apoptotic effects. In recent years, extensive research has been conducted to explore the physiological significance of H<sub>2</sub>S and its potential applications in developing prodrugs. To further unravel the biological and clinical potential of H<sub>2</sub>S, H<sub>2</sub>S donors have gained widespread utilization. These compounds facilitate our understanding of the specific functional aspects governed by H<sub>2</sub>S and hold promise as potential therapeutic agents. Therefore, it is necessary to study H<sub>2</sub>S as a delivery vehicle at the cellular and in vivo levels. This review provides an overview of advancements made over the past five years regarding H<sub>2</sub>S donors and their applications in biology, encompassing indirectly released donors of carbonyl sulfide (COS), directly released small molecule donors, Nanocomposite scaffolds, and hydrogels.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400817"},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Navigating the deuteration landscape: innovations, challenges, and clinical potential of deuterioindoles.
IF 2.6 4区 生物学
ChemBioChem Pub Date : 2024-12-10 DOI: 10.1002/cbic.202400837
Li Zhang, Shujingwei Zhang, Yang Zhang, Bo Liu, Xiang Li, Bo Han
{"title":"Navigating the deuteration landscape: innovations, challenges, and clinical potential of deuterioindoles.","authors":"Li Zhang, Shujingwei Zhang, Yang Zhang, Bo Liu, Xiang Li, Bo Han","doi":"10.1002/cbic.202400837","DOIUrl":"https://doi.org/10.1002/cbic.202400837","url":null,"abstract":"<p><p>Indoles, pivotal to the realm of drug discovery, underpin numerous FDA-approved therapeutics. Despite their clinical benefits, pharmacokinetic and toxicity concerns have occasionally hampered their broader application. A notable advancement in this domain is the substitution of hydrogen atoms with deuterium, known as deuterium modification, which significantly enhances the pharmacological properties of these compounds. This review elucidates the progression of deuterium chemistry, culminating in approval of Deutetrabenazine in 2017. This milestone has catalyzed additional research into deuterated indoles, such as Dosimertinib, which have demonstrated enhancements in stability, toxicity profiles, and therapeutic efficacy. Moreover, the review addresses challenges and patent issues in the synthesis of deuterated indoles and highlights their potential applications in precision medicine. In the future, deuterated indoles may positively impact therapy and contribute to advances in precision medicine through molecular engineering.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400837"},"PeriodicalIF":2.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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