ChemBioChem最新文献_第2页

Charge-Based Discrimination of Amyloids Using an Amyloid-Targeting Chemiluminescent Probe. 淀粉样蛋白靶向化学发光探针在淀粉样蛋白电荷识别中的应用。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.202600008

Jeewon Chung, R Erin Jessup, Jerry Yang

{"title":"Charge-Based Discrimination of Amyloids Using an Amyloid-Targeting Chemiluminescent Probe.","authors":"Jeewon Chung, R Erin Jessup, Jerry Yang","doi":"10.1002/cbic.202600008","DOIUrl":"https://doi.org/10.1002/cbic.202600008","url":null,"abstract":"Fluorescent probes have been widely developed for detecting amyloid biomarkers associated with neurodegenerative diseases (NDs). The requirement for external light for excitation, however, limits their utility for research and in vivo applications. Chemiluminescent probes, which use chemical reactions to generate emissive excited states rather than externally applied light, may offer a promising alternative to overcome some limitations for optical imaging of amyloid biomarkers. Here, we designed and synthesized a chemiluminescent amyloid-binding probe (CLIP-1) and evaluated its capability to label three amyloid biomarkers for NDs-amyloid β (Aβ), α-synuclein (α-syn), and tau protein aggregates. We found that CLIP-1 exhibits markedly enhanced chemiluminescence (CL) in aqueous solution when activated by ambient oxygen in the presence of aggregated Aβ, whereas little to no emission is observed in the absence of aggregates or in the presence of monomeric Aβ. Additionally, CLIP-1 displayed a different wavelength of emission when bound to tau aggregates compared to Aβ or α-syn aggregates, which we attribute to differences in the relative overall charge of the amyloids at neutral pH. Imaging of brain slices confirmed enhanced CL of CLIP-1 in an Alzheimer's disease mouse model, highlighting the potential for amyloid-targeting chemiluminescent probes as new tools for aiding in diagnosis of amyloid-associated neurodegenerative diseases.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e202600008"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing S. aureus tRNA Guanine Transglycosylase for Efficient Site-Specific Covalent Labeling of DNA. 利用金黄色葡萄球菌tRNA鸟嘌呤转糖基酶进行DNA的高效位点特异性共价标记。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.202500971

Zhenglong Zhai, Evan McCormack, Alexander Harjung, Neal K Devaraj

{"title":"Harnessing S. aureus tRNA Guanine Transglycosylase for Efficient Site-Specific Covalent Labeling of DNA.","authors":"Zhenglong Zhai, Evan McCormack, Alexander Harjung, Neal K Devaraj","doi":"10.1002/cbic.202500971","DOIUrl":"https://doi.org/10.1002/cbic.202500971","url":null,"abstract":"Site-specific DNA modification enables the construction of functional nucleic acid architectures for imaging, diagnostics, and information encoding. Bacterial tRNA guanine transglycosylases (TGTs) have been shown to exchange guanine-34 in cognate tRNAs for prequeuosine1 (preQ1) analogs. Escherichia coli TGT (EcTGT) has been widely harnessed for site-specific RNA labeling. However, EcTGT exhibits slow labeling kinetics and lower efficiency on DNA. Here, we demonstrate that TGT from Staphylococcus aureus (SaTGT) is a superior enzyme for DNA modification. Like EcTGT, SaTGT recognizes only the anticodon stem-loop and efficiently modifies the DNA hairpin dECYMH, whereas EcTGT shows no appreciable modification. Using the S. aureus tRNAHis-based hairpin as a starting point, we identified the most promising scaffolds. Rational loop optimization yielded dSAH-6, which is labeled by SaTGT with near-quantitative conversion under standard conditions. Side-by-side comparisons revealed that SaTGT outperforms EcTGT in both yield and kinetics across multiple preQ1 derivatives and hairpin sequences. Importantly, the optimized dSAH-6 sequence enables robust labeling at 5'-, 3'-, or internal positions in longer ssDNA constructs and supports efficient multi-site incorporation. These findings significantly expand the DNA-TAG toolbox and highlight how natural variation in TGT substrate promiscuity can be exploited for improved nucleic acid modification technologies.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e202500971"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design of a Multivalent RNA Combining Structural Motifs Tailored to Multiple Domains of Fused in Sarcoma for Potent Inhibition of Aggregation. 合理设计一种多价RNA结合结构基序，以适应肉瘤中融合的多个结构域，有效抑制聚集。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.202500568

Nobuaki Kuroiwa, Fumika Sakaue, Motoki Miura, Ayano Go, Ryo Iwase, Rintaro Iwata Hara, Nobuo Sanjo, Kumiko Ui-Tei, Takanori Yokota

{"title":"Rational Design of a Multivalent RNA Combining Structural Motifs Tailored to Multiple Domains of Fused in Sarcoma for Potent Inhibition of Aggregation.","authors":"Nobuaki Kuroiwa, Fumika Sakaue, Motoki Miura, Ayano Go, Ryo Iwase, Rintaro Iwata Hara, Nobuo Sanjo, Kumiko Ui-Tei, Takanori Yokota","doi":"10.1002/cbic.202500568","DOIUrl":"https://doi.org/10.1002/cbic.202500568","url":null,"abstract":"Fused in sarcoma (FUS) is an RNA-binding protein whose pathological aggregation, driven by aberrant phase separation, is implicated in amyotrophic lateral sclerosis (ALS). Although RNA molecules can modulate the FUS phase behavior, identifying highly effective sequences remains challenging because of FUS's multiple low-specificity RNA-binding domains. In this study, we rationally designed a 65-mer RNA, U1'+TERRA, by combining a stem-loop-GGU motif and a G-quadruplex (G4) structure, each known to interact with distinct FUS domains. U1'+TERRA exhibited strong binding affinity and effectively inhibited FUS aggregation in vitro. We introduced 2'-O-methyl modifications, generating (U1'+TERRA)-2'-OMe, which retained structural integrity and demonstrated resistance to nuclease degradation to enhance biological stability. Notably, (U1'+TERRA)-2'-OMe suppressed FUS aggregation even at a low concentration. These findings suggested that multivalent RNA constructs with rationally arranged motifs can serve as potent inhibitors of FUS aggregation. Our approach highlights the potential of structure-guided RNA engineering for the development of nucleic acid therapeutics targeting RNA-binding proteins involved in neurodegenerative diseases, such as ALS.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e202500568"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic Cell-Based Artificial Stem Cell Niches for Hematopoietic Stem Cell Differentiation. 基于合成细胞的人工干细胞龛用于造血干细胞分化。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.202500864

Ivaylo Balabanov, Sara Madureira, Anna Burgstaller, Maja Fehlberg, Nils Piernitzki, Nurzhan Abdukarimov, Franziska Lautenschläger, Oskar Staufer

{"title":"Synthetic Cell-Based Artificial Stem Cell Niches for Hematopoietic Stem Cell Differentiation.","authors":"Ivaylo Balabanov, Sara Madureira, Anna Burgstaller, Maja Fehlberg, Nils Piernitzki, Nurzhan Abdukarimov, Franziska Lautenschläger, Oskar Staufer","doi":"10.1002/cbic.202500864","DOIUrl":"10.1002/cbic.202500864","url":null,"abstract":"Hematopoietic stem cells (HSCs) receive a combination of biochemical and biomechanical signals within the bone marrow that guide their differentiation process. These include soluble factor signaling with cytokines, cellular confinement in the stem cell niche, and contact-dependent receptor-ligand interactions with stromal cells. Recreating this complex microenvironment in vitro is a principal engineering challenge for regenerative therapies and tissue engineering. While cytokines can be easily supplemented in vitro, and several systems for confined HSC culture have been developed, integrating receptor-based intercellular interactions found in stem cell niches has only been achieved with quantitatively undefined heterotypic co-cultures. We report here the development of microwell-based systems that integrate synthetic cells to mimic receptor-ligand interactions within hematopoietic niches. The synthetic cells are based on droplet-supported lipid bilayers (dsLBs) with cytomimetic stiffness and present Notch receptor ligands on a laterally mobile lipid membrane. We show the system's applicability to individually tune the three signaling axes: soluble factors, confinement, and intercellular interactions for HSC differentiation. Introducing synthetic cells as an alternative to coculture and feeder cells opens the possibility to engineer precisely defined HSC niches with adjustable biochemical and biomechanical properties.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e202500864"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13096856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of BI-10: An Unexplored Broad-Spectrum Antimicrobial Benzimidazole Derivative Targeting Bacterial Membrane. BI-10的发现：一种未开发的广谱抗菌苯并咪唑衍生物靶向细菌膜。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.70316

Biplab Sarkar, Rakshit Manhas, Arti Rathore, Jyoti Kumari, Shifa Firdous, Avisek Mahapa

{"title":"Discovery of BI-10: An Unexplored Broad-Spectrum Antimicrobial Benzimidazole Derivative Targeting Bacterial Membrane.","authors":"Biplab Sarkar, Rakshit Manhas, Arti Rathore, Jyoti Kumari, Shifa Firdous, Avisek Mahapa","doi":"10.1002/cbic.70316","DOIUrl":"10.1002/cbic.70316","url":null,"abstract":"Antimicrobial resistance (AMR) has emerged as a global healthcare crisis, necessitating the discovery of potent antibacterial agents. In the present investigation, we report the discovery, in vitro and ex vivo antibacterial efficacy studies, and mechanism of action of an unexplored benzimidazole derivative (BI-10), a promising broad-spectrum antibacterial agent with significant efficacy against Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA). BI-10 demonstrated a minimum Inhibitory Concentration (MIC) of 2.4 μg/ml (6.25 µM) against these priority pathogens. In vitro assessments revealed that BI-10 possessed rapid bactericidal activity, anti-biofilm potential and showed synergistic interactions with conventional antibiotics. Ex vivo efficacy studies using various mammalian cell lines demonstrated strong intracellular killing. Moreover, BI-10 showed the ability to prevent both adhesion and invasion of pathogens in different mammalian cell infection models. The membrane-disrupting nature of BI-10 against both pathogens was established using scanning electron microscopy, membrane permeability, depolarization, and integrity assays. Multiple in silico analyses further demonstrated drug-likeness and the biocompatibility profile of BI-10. Altogether, our findings establish BI-10 as a potent, broad-spectrum antimicrobial agent with robust in vitro and ex vivo efficacy and bacterial cell membrane-disrupting activity. This study highlights BI-10 as a valuable antibacterial lead molecule for the development of a new antimicrobial agent against multidrug-resistant pathogens.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e70316"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luminescent Fe₃O₄ Nanohybrid for Intra-Cellular Imaging and Combinatorial Chemo-Photothermal Therapy in Cancer. 发光Fe3O4纳米杂化物在肿瘤细胞内成像和化学光热联合治疗中的应用。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.202500886

Bijaideep Dutta, Sonali Gupta, Sharanaya Purandare, Premlata Bind, Rudheer Bapat, Nand Kisore Prasad, Kanhu Charan Barick, P A Hassan

{"title":"Luminescent Fe3O4 Nanohybrid for Intra-Cellular Imaging and Combinatorial Chemo-Photothermal Therapy in Cancer.","authors":"Bijaideep Dutta, Sonali Gupta, Sharanaya Purandare, Premlata Bind, Rudheer Bapat, Nand Kisore Prasad, Kanhu Charan Barick, P A Hassan","doi":"10.1002/cbic.202500886","DOIUrl":"10.1002/cbic.202500886","url":null,"abstract":"Highly stable water dispersible carbon dot decorated hybrid magnetic nanoparticles (FCDs) that combines magnetic Fe3O4 nanocrystals and fluorescent carbon dots (GUCDs) were successfully synthesized using a facile strategy. The resultant Fe3O4@GUCDs (FCDs) hybrid NPs not only validate excellent magnetic responsive properties (Ms = 33.5 emu g-1) from the magnetic (Fe3O4) core, but also exhibit intriguing photo luminescent properties, including excellent photo stability from the CDs produced from glucose-urea mixture. The FCDs can enter the intracellular region and illuminate breast cancer cells (MCF-7), and showed a time and dose-dependent localization kinetics. Meanwhile, the presence of hydrophilic surface functional groups in FCDs contributes to the excellent stability in aqueous solutions as well as loading of the anti-cancer drug doxorubicin with a loading capacity of 22 mg g-1. More importantly, the FCDs can absorb NIR irradiation followed by their conversion to heat energy to achieve photothermal modality. Thus, such nanostructured hybrid NPs (FCDs) demonstrate great potential towards advanced single platform nano-theranostics with combinatorial chemo-photothermal therapy.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e202500886"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13096865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postsynthetic Construction and Properties of Oligonucleotides Containing Fluorescent 7-Arylethenyl-7-Deazaguanosine Derivatives. 含荧光7-芳基-7-去氮鸟苷衍生物的寡核苷酸的合成后结构和性质。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.70337

Miho Kawaguchi, Yasufumi Fuchi, Yuta Ito, Yoshiyuki Hari

{"title":"Postsynthetic Construction and Properties of Oligonucleotides Containing Fluorescent 7-Arylethenyl-7-Deazaguanosine Derivatives.","authors":"Miho Kawaguchi, Yasufumi Fuchi, Yuta Ito, Yoshiyuki Hari","doi":"10.1002/cbic.70337","DOIUrl":"10.1002/cbic.70337","url":null,"abstract":"Fluorescent nucleobase analogs (FBAs) are widely used probes for nucleic acid analysis. In this study, we designed, synthesized, and investigated fluorescent 7-arylethenyl-7-deazaguanosine derivatives as novel FBAs conjugated to molecular rotor structures. More than 10 types of oligonucleotides (ONs) containing the designed FBAs were constructed via the postsynthetic modification of 7-iodo-7-deazaguanosine using Suzuki-Miyaura cross-coupling. This approach allowed the efficient construction of a guanosine-type FBA library. The duplex stability of ONs containing 7-arylethenyl-7-deazaguanosine was slightly lower than that of canonical ONs. In contrast, naphthylethenyl-modified derivatives unexpectedly showed enhanced triplex stability compared with canonical DNA triplexes. The synthesized FBAs exhibited fluorescence emissions in the visible region, and the fluorescence spectra of the duplexes and triplexes containing 7-arylethenyl-7-deazaguanosine exhibited enhanced emissions, which varied depending on the aryl substituent. In particular, upon G·TA triplex formation, 1-or 2-naphthylethenyl-modified derivatives acted as a molecular rotor and showed strong fluorescence enhancement in a sequence-selective manner.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e70337"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Yield Expression of Arylmalonate Decarboxylase in Escherichia coli Through High-Cell-Density Cultivation Strategies. 通过高密度培养策略在大肠杆菌中高产表达芳基丙二酸脱羧酶

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.70339

Jan Gerstenberger, Timm Werbilo, Robert Kourist, Selin Kara

{"title":"High-Yield Expression of Arylmalonate Decarboxylase in Escherichia coli Through High-Cell-Density Cultivation Strategies.","authors":"Jan Gerstenberger, Timm Werbilo, Robert Kourist, Selin Kara","doi":"10.1002/cbic.70339","DOIUrl":"10.1002/cbic.70339","url":null,"abstract":"This study develops and optimizes a high-cell-density cultivation (HCDC) of E. coli BL21 (DE3) to improve enzyme production utilizing DASGIP multibioreactor systems. By shifting from traditional shake flask methods to HCDC, we observed a 43-fold increase in biomass production, with a comparable mass-specific activity of the cell-free extract. The HCDC demonstrated a 30-fold increase in the active, soluble expression of arylmalonate decarboxylase (AMDase) from Bordetella bronchiseptica (BbAMDase) compared to conventional methodology. The protocol was designed to be readily adapted to various expression approaches and to provide a robust foundation for broader use in recombinant protein production. The HCDC was achieved with a linear glucose feed to promote robust cell growth. We optimized the glycerol feeding strategy during isopropyl β-D-1-thiogalactopyranoside (IPTG) induction to maximize AMDase production. Throughout the induction phase, we monitored both soluble expression yield and enzymatic activity, aiming to establish a process that is not only profitable but also efficient and stable.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e70339"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13096762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Peripheral Myelin Protein 2 and Charcot-Marie-Tooth Disease or Structural Missense Variants Show Different Binding to Myelin-Like Lipid Monolayers. 人外周髓磷脂蛋白2和腓骨肌病或结构错义变异显示髓磷脂样脂质单分子层的不同结合。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.202500947

Florian Arndt Schöffmann, Md Abdus Shukur Imran, Christian Schwieger, Øystein Hetland, Vanessa Jerschabek, Arne Raasakka, Petri Kursula, Dariush Hinderberger

{"title":"Human Peripheral Myelin Protein 2 and Charcot-Marie-Tooth Disease or Structural Missense Variants Show Different Binding to Myelin-Like Lipid Monolayers.","authors":"Florian Arndt Schöffmann, Md Abdus Shukur Imran, Christian Schwieger, Øystein Hetland, Vanessa Jerschabek, Arne Raasakka, Petri Kursula, Dariush Hinderberger","doi":"10.1002/cbic.202500947","DOIUrl":"10.1002/cbic.202500947","url":null,"abstract":"The peripheral myelin protein P2 (also known as PMP2/FABP8) has two basic functions in vivo: the transport of fatty acids within the cell to the target compartments and the adhesion of lipid bilayers in the peripheral nervous system myelin to each other. In this work, we focus on the membrane-binding properties of P2 and its Charcot-Marie-Tooth disease variants. Experiments were carried out on lipid monolayers at the air-water interface as a membrane model system, with the lipid composition of the cytoplasmic leaflet of myelin. Our study provides quantitative data on the membrane affinity of P2 and its disease-linked or structure-based missense variants toward the native lipid membrane and shows affinity differences due to single P2 point mutations. Phospholipid monolayer surface pressure measurements are supported by epifluorescence microscopy, which not only shows the adhesive property of all P2 variants but also the complex cross-linking property of the wild-type P2. An analysis of transcriptomics databases confirms expression of P2 in human, but not mouse, central nervous system nonneuronal cells. Taken together, our work further confirms the role of P2 in binding myelin-like lipid membranes as well as its direct effects on lipid membrane properties.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e202500947"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13096861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147727834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antisense Oligonucleotide Releasing Cassette for Conditional Targeted RNA Degradation. 条件靶向RNA降解的反义寡核苷酸释放盒。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2026-04-28 DOI: 10.1002/cbic.202500899

Valeriia S Drozd, Muhammad S Anwar, Adeliia A Salimova, Daria S Rybalko, Tatiana V Vasileva, Victor V Tatarskiy, Dmitry M Kolpashchikov, Ahmed A Eldeeb

{"title":"Antisense Oligonucleotide Releasing Cassette for Conditional Targeted RNA Degradation.","authors":"Valeriia S Drozd, Muhammad S Anwar, Adeliia A Salimova, Daria S Rybalko, Tatiana V Vasileva, Victor V Tatarskiy, Dmitry M Kolpashchikov, Ahmed A Eldeeb","doi":"10.1002/cbic.202500899","DOIUrl":"https://doi.org/10.1002/cbic.202500899","url":null,"abstract":"Conventional single-stranded antisense oligonucleotides (ASOs) work by directly binding target mRNA, which limits their use to genes that need to be silenced without affecting healthy cells. To address this limitation and achieve conditional gene silencing specifically within target cells, we developed. This system activates its RNA-cleaving function only upon binding to a specific biomarker nucleic acid sequence followed by RNase H-dependent cleavage of the targeted RNA. Under cell-free conditions, ARC showed 3-fold greater RNA cleavage efficiency in the presence of the biomarker as compared to its absence. Intriguingly, in the ARC-treated GFP-expressing K562 cell line a 45% reduction in GFP fluorescence was achieved in the presence of the biomarker sequence, which was 5.3-fold greater than the reduction observed when it was absent. This ARC's conditional ASO release offers a new mechanism for safely targeting essential or vital genes exclusively in cells that produce specific RNA biomarkers. Our findings indicate that the future in the field of nucleic acid nanotechnology will critically depend on two key advancements: the development of appropriate intracellular delivery systems and the establishment of robust predictive tools for accurately modeling the thermodynamic behavior of complex nanostructures, including those incorporating diverse chemical modifications.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"27 8","pages":"e202500899"},"PeriodicalIF":2.8,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147758009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0