ChemBioChem最新文献_第2页

Front Cover: Recent Advances in Biocatalytic and Chemoenzymatic Synthesis of Oligonucleotides (ChemBioChem 9/2025) 封面：生物催化和化学酶合成寡核苷酸的最新进展（ChemBioChem 9/2025）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-12 DOI: 10.1002/cbic.202580901

Pierre Nicolas Bizat, Nazarii Sabat, Marcel Hollenstein

引用次数: 0

The Advancement of Prime Editing Technology. 主要编辑技术的进步。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-11 DOI: 10.1002/cbic.202500193

Lu Zhang, Dongdong Zhao, Zhandong Wei, Xiao Zhu, Taixin Sha, Wenzhu Tang, Changhao Bi, Xueli Zhang

引用次数: 0

Tagatose 1,6-bisphosphate aldolases, the unloved DHAP-aldolases. 塔格糖1,6-二磷酸醛缩酶，不受欢迎的dhap醛缩酶。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-10 DOI: 10.1002/cbic.202500285

Christine Guérard-Hélaine, Léo Paulat, Marielle Lemaire, Virgil Hélaine

引用次数: 0

Cyanophenylalanine as an Infrared Probe for Iron-Sulfur Cluster Redox State in Multi-Centre Metalloenzymes. 氰苯丙氨酸作为多中心金属酶铁-硫团簇氧化还原态的红外探针。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-10 DOI: 10.1002/cbic.202500251

Zehui Duan, Jiaao Wei, Stephen B Carr, Miguel Ramirez, Rhiannon M Evans, Philip A Ash, Patricia Rodriguez-Macia, Amit Sachdeva, Kylie Alison Vincent

{"title":"Cyanophenylalanine as an Infrared Probe for Iron-Sulfur Cluster Redox State in Multi-Centre Metalloenzymes.","authors":"Zehui Duan, Jiaao Wei, Stephen B Carr, Miguel Ramirez, Rhiannon M Evans, Philip A Ash, Patricia Rodriguez-Macia, Amit Sachdeva, Kylie Alison Vincent","doi":"10.1002/cbic.202500251","DOIUrl":"https://doi.org/10.1002/cbic.202500251","url":null,"abstract":"We demonstrate that the noncanonical amino acid, para-cyanophenylalanine (CNF), when incorporated into metalloproteins, functions as an infrared spectroscopic probe for the redox state of iron sulfur clusters, offering a strategy for determining electron occupancy in the electron transport chains of complex metalloenzymes. We observe a redshift of ≈ 1 - 2 cm-1 in the NC stretching frequency following reduction of spinach ferredoxin modified to contain CNF close to its [2Fe-2S] centre, and this shift is reversed on re-oxidation. We extend this to CNF positioned near to the proximal [4Fe-4S] cluster of the [FeFe] hydrogenase from Desulfovibrio desulfuricans. In combination with a distal [4Fe-4S] cluster and the [4Fe-4S] cluster of the active site 'H-cluster' ([4Fe-4S]H), the proximal cluster forms an electron relay connecting the active site to the surface of the protein. Again, we observe a reversible shift in wavenumber for CNF following cluster reduction in either apo-protein (containing the iron-sulfur clusters but lacking the active site) or holo-protein with intact active site, demonstrating general applicability of this approach to studying complex metalloenzymes.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500251"},"PeriodicalIF":2.6,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and evaluation of pseudoglucosinolates (psGSLs) releasing isothiocyanates (ITCs) in the presence of azoreductases. 在偶氮还原酶存在下释放异硫氰酸酯的假硫代葡萄糖苷（psGSLs）的合成及评价。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-09 DOI: 10.1002/cbic.202500152

Aishi Chakrabarti, Charity S G Ganskow, Mervic D Kagho, Claire C Jimidar, Lorenz Wiese, Neslihan Beyazit, Ulrike Beutling, Magarita Seeger, Silvana Smits, Stella Nyström, Anett Schallmey, Anne Farewell, Mark Brönstrup, Philipp Klahn

引用次数: 0

Hydrocarbon stapling enables improvement of antimicrobial activity and proteolytic stability of host-defense peptide ocellatin-3N. 钉接碳氢化合物可以提高宿主防御肽ocellatin-3N的抗菌活性和蛋白水解稳定性。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-09 DOI: 10.1002/cbic.202500204

Hao Yang, Fei Yuan, Guangxu Xie, Yinxue Fu, Jia Mi, Longjie Yu, Weijia Liu, Yulei Li

引用次数: 0

Biosynthesis of the fungal cyclic lipodepsipeptide pleosporacin, a new selective inhibitor of the phytopathogen Botrytis cinerea. 真菌环脂肽多孢霉素的生物合成——一种新的植物病原菌葡萄孢菌的选择性抑制剂。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-09 DOI: 10.1002/cbic.202500315

Carsten Wieder, Rainer Wiechert, Alexander Yemelin, Louis Pergaud Sandjo, Eckhard Thines, Till Opatz, Anja Schüffler

引用次数: 0

Gas Microbubble Formation via Near-Infrared Femtosecond Laser Ablation in Live Cells. 通过近红外飞秒激光烧蚀在活细胞中形成气体微泡。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-09 DOI: 10.1002/cbic.202500086

Kazunori Okano, Rieko Aida, Hayato Suwa, Naomi Tanga, Koichro Kishima, Yaxiaer Yalikun, Yoichroh Hosokawa, Hiromi Hagiwara

{"title":"Gas Microbubble Formation via Near-Infrared Femtosecond Laser Ablation in Live Cells.","authors":"Kazunori Okano, Rieko Aida, Hayato Suwa, Naomi Tanga, Koichro Kishima, Yaxiaer Yalikun, Yoichroh Hosokawa, Hiromi Hagiwara","doi":"10.1002/cbic.202500086","DOIUrl":"https://doi.org/10.1002/cbic.202500086","url":null,"abstract":"Focused near-infrared femtosecond (NIR fs) laser irradiation induces efficient photochemical breakdown of intracellular molecules, resulting in the formation of gas microbubbles that ultimately lead to cell death and removal. This study characterizes these microbubbles using high-speed imaging. Microbubbles generated within cells by single-pulse irradiation were consistently larger than those formed in the surrounding culture medium across a broad pulse energy range. Notably, the energy threshold for intracellular microbubble formation was several tens of nanojoules lower than in the culture medium, enabling precise cell ablation without inducing extracellular bubbling. Repeated pulse irradiation at 1 kHz frequency further reduced the energy threshold and increased bubble size, with saturation observed after approximately 30 pulses. Near-threshold pulse irradiation was then employed to develop a precise method for investigating cellular motility in vitro. Cell-free zones were created within cell monolayers, enabling motile cells to migrate into the cleared zones. This method was validated using the A549 lung cancer cell line and the plant flavonoid apigenin, which exhibited dose-dependent reduction in A549 cell motility. These findings highlight the method's potential for evaluating cellular behaviours and screening chemical compounds.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500086"},"PeriodicalIF":2.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultraviolet Ozone Pretreatment Enhances selective PETase Biodegradation of Textiles Compared to Sunlight and Alkali Treatments. 与日光和碱处理相比，紫外臭氧预处理提高了纺织品peta酶的选择性生物降解。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-08 DOI: 10.1002/cbic.202500004

Sariah Giraldo-Narcizo, Antonio Guerrero, Ana Maria Sanchez-Perez

{"title":"Ultraviolet Ozone Pretreatment Enhances selective PETase Biodegradation of Textiles Compared to Sunlight and Alkali Treatments.","authors":"Sariah Giraldo-Narcizo, Antonio Guerrero, Ana Maria Sanchez-Perez","doi":"10.1002/cbic.202500004","DOIUrl":"https://doi.org/10.1002/cbic.202500004","url":null,"abstract":"The rapid growth of the fashion industry has led to increasing textile waste, exacerbating environmental pollution and climate change. To support sustainability and circular economy goals, this study investigates the enzymatic degradation of cotton/polyethylene terephthalate (PET) mixed textiles using PETase, comparing wild-type (WT) and mutant (MUT S238F/W159H) variants. To improve enzyme accessibility, we evaluated three pretreatment strategies: alkali treatment, ultraviolet-ozone (UVO) exposure, and natural sunlight weathering. The effects were assessed by measuring textile weight loss, surface morphology (SEM), infrared spectroscopy (FTIR), and yields of terephthalic acid (TPA) and mono-(2-hydroxyethyl) terephthalic acid (MHET). Alkali treatment produced the highest weight loss, while UVO pretreatment moderately degraded textiles and significantly enhanced enzymatic TPA production. In contrast, prolonged sunlight exposure had negligible effects. ^1H NMR analysis of supernatants confirmed the formation of oxidized PET products following UVO exposure, indicating surface chemical modifications that increase enzymatic susceptibility. Our results reveal differential effects on PET and cotton fibers, highlighting UVO as a promising, selective pretreatment for mixed textile waste. This study demonstrates the potential of combining photochemical oxidation and enzymatic processes for targeted PET degradation, contributing to more efficient textile recycling strategies.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500004"},"PeriodicalIF":2.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Organometallic Gold(III) Complexes as Precision Scaffolds for Next-Generation Therapeutic and Imaging Agents. 利用有机金属金（III）配合物作为新一代治疗和显像剂的精密支架。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-08 DOI: 10.1002/cbic.202500347

Sophie R Thomas, Riccardo Bonsignore

{"title":"Harnessing Organometallic Gold(III) Complexes as Precision Scaffolds for Next-Generation Therapeutic and Imaging Agents.","authors":"Sophie R Thomas, Riccardo Bonsignore","doi":"10.1002/cbic.202500347","DOIUrl":"https://doi.org/10.1002/cbic.202500347","url":null,"abstract":"Gold(III) organometallic complexes, particularly cyclometalated Au(III) compounds, have emerged as powerful tools in catalysis and bioinorganic chemistry, offering unique reactivity distinct from their Au(I) counterparts. Among their most interesting transformations, C-S cross-coupling reactions have become a selective strategy for cysteine arylation, enabling site-specific modifications of peptides and proteins. This review provides a comprehensive overview of cyclometalated Au(III) complexes in C-S bond formation, detailing the mechanistic insights, ligand effects, and electronic factors that dictate their reactivity. The role of ancillary ligands in tuning stability and selectivity is critically assessed, alongside advancements in structural modifications that enhance catalytic efficiency. Beyond fundamental C-S cross-coupling, we explore the broader applications of these gold(III) complexes, including enzyme inhibition, metabolic disruption, and transmembrane protein modulation, with implications in anticancer therapy, antimicrobial strategies, and in vivo catalytic transformations. By bridging fundamental organometallic reactivity with innovative biomedical applications, this review highlights the potential of cyclometalated Au(III) complexes as next-generation catalysts for both synthetic and therapeutic innovations.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500347"},"PeriodicalIF":2.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0