ChemBioChem最新文献_第3页

Optimization and Application of the Purified Cell-free System.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-03 DOI: 10.1002/cbic.202500176

Chao Liao, Hengrun Li, Haotian Zheng, Chaofeng Li, Liangxu Liu, Jiawei Wang, Jun Ni

引用次数: 0

Phase separation in biochemical and biological systems: Implications for disease pathogenesis.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-03 DOI: 10.1002/cbic.202400883

Raj Dave, Kshipra Pandey, Ritu Patel, Raghu Solanki, Nidhi Gour, Dhiraj Bhatia

引用次数: 0

Induction and manipulation of biomolecular condensates through spatially heterogeneous solution conditions.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-03 DOI: 10.1002/cbic.202500044

Alexander Kai Buell

引用次数: 0

SAAP-148 oligomerizes into a hexamer forming a hydrophobic inner core.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-01 DOI: 10.1002/cbic.202500112

Nermina Malanovic, Aden Hodzic, Djenana Vejzovic, Altea Topciu, Kirill Kuhlmann, Raj Kumar, Maria Andrea Mroginski, Alejandra Andrea de Miguel, Pia Hofmann, Klaus Zangger, Markus Weingarth, Robert A Cordfunke, Jan W Drijfhout, Peter Nibbering, Michal Belicka, Karl Lohner

{"title":"SAAP-148 oligomerizes into a hexamer forming a hydrophobic inner core.","authors":"Nermina Malanovic, Aden Hodzic, Djenana Vejzovic, Altea Topciu, Kirill Kuhlmann, Raj Kumar, Maria Andrea Mroginski, Alejandra Andrea de Miguel, Pia Hofmann, Klaus Zangger, Markus Weingarth, Robert A Cordfunke, Jan W Drijfhout, Peter Nibbering, Michal Belicka, Karl Lohner","doi":"10.1002/cbic.202500112","DOIUrl":"https://doi.org/10.1002/cbic.202500112","url":null,"abstract":"Human cathelicidin LL-37 is a widely studied antimicrobial 37-mer peptide with various ascribed functions, that serves as a template for designing novel peptides. Its derivative, the 24-mer SAAP-148, is highly effective in vitro in eradicating multidrug-resistant bacteria, persistent cells, and biofilms, without inducing resistance. SAAP-148 is characterized by a high cationic charge (+11) and 46% hydrophobicity, which, once the peptide folds into an alpha helix, forms a wide hydrophobic face. This highly amphipathic nature facilitates on the one hand its insertion into the membrane's fatty acyl chain region and on the other hand it´s interaction with anionic membrane components, which contributes to its mode of action in killing bacteria. However, the contributions of the secondary and quaternary structures remain underexplored. To address this, we conducted a study using anionic and zwitterionic membrane models, applying circular dichroism, NMR spectroscopy, X-ray scattering, AlphaFold 3 and molecular dynamics simulations. Our results reveal that SAAP-148 adopts a stable hexameric bundle composed of three parallel dimers, creating a hydrophobic core of aromatic residues. This structure is retained at the membrane interface, where MD simulations suggest the formation of a fiber-like structure on anionic membranes, most likely driven by exposed cationic side chains.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500112"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of endogenous sequences interacting with METTL3/METTL14 RNA methyltransferase.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-01 DOI: 10.1002/cbic.202500006

Miki Imanishi, Tamaki Endoh, Yiwei Ling, Shujiro Okuda

{"title":"Identification of endogenous sequences interacting with METTL3/METTL14 RNA methyltransferase.","authors":"Miki Imanishi, Tamaki Endoh, Yiwei Ling, Shujiro Okuda","doi":"10.1002/cbic.202500006","DOIUrl":"https://doi.org/10.1002/cbic.202500006","url":null,"abstract":"N6-methyladenosine (m6A) is the most abundant RNA modification in mRNA and regulates various biological processes. The RNA-binding properties of m6A writer proteins play an important role in determining RNA modification sites. METTL3 and METTL14 form the core of the m6A writer complex, with METTL3 as the catalytic methyltransferase and METTL14 as the RNA-binding scaffold. Thus far, the comprehensive RNA binding properties of METTL3/14 remain unknown. Using RNA-capturing microsphere particles (R-CAMPs), immobilizing RNA fragments derived from endogenous RNAs of human lung carcinoma cells, we isolated RNA fragments that interacted with the METTL3/14 methyltransferase domain. Bioinformatics analysis revealed that the pool of isolated sequences contained significantly more regions with the potential to form RNA G-quadruplexes (rG4s) than the randomly extracted RNA sequence pool and that the (GGA) repeat sequences were most enriched. CD spectroscopy, gel mobility shift assays, and methylation experiments demonstrated that METTL3/14 binds to RNA sequences containing (GGA) repeats that form rG4 structure much stronger than RNAs that do not form rG4s. This study shows the potential of RNA G-quadruplex structures as a modulator of epitranscriptomic modification of m6As.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500006"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioapplications of Cell Membrane Engineering with DNA Nanotechnology.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-01 DOI: 10.1002/cbic.202500066

Jingyi Zheng, Jian-Rong Zhang, Sai Bi, Qianying Zhang, Jun-Jie Zhu

{"title":"Bioapplications of Cell Membrane Engineering with DNA Nanotechnology.","authors":"Jingyi Zheng, Jian-Rong Zhang, Sai Bi, Qianying Zhang, Jun-Jie Zhu","doi":"10.1002/cbic.202500066","DOIUrl":"https://doi.org/10.1002/cbic.202500066","url":null,"abstract":"Engineering the cell surface has emerged as a significant method for manipulating cell behavior and determining cell fate. Regulating the composition or structure of cell membranes has the potential to impact the essential roles they play in biointerfacing, signal transduction, and compartmentalization. This presents significant prospects for the advancement of cell-based biomedicine. DNA nanotechnology has emerged as a promising experimental toolbox for cell membrane engineering, owing to its high programmability and excellent biocompatibility. Non-genetic strategies based on DNA nanotechnology for programming cell membranes have seen rapid growth over the past decade, showing promising prospects for the cell-based therapeutic diagnostic. In this review, we first introduced the nongenetic-based strategies for the functionalization of cell membranes. We also highlighted and summarized the biological applications of DNA nanotechnology in cell membrane engineering, including molecular sensing, modulation of cell membrane signaling pathways and intercellular interactions, and establishment of transmembrane channels. Finally, we have discussed the challenges and prospects of DNA nanotechnology in cell membrane engineering.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500066"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile Conjugation Method of CpG-ODN Adjuvant to Intact Virions for Vaccine Development.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-03-31 DOI: 10.1002/cbic.202400988

Alexandra Maalouf, Hengwei Zhu, Anika Zaman, Neena Carpino, Janet Hearing, Surita Bhatia, Isaac Sheridan Carrico

{"title":"Facile Conjugation Method of CpG-ODN Adjuvant to Intact Virions for Vaccine Development.","authors":"Alexandra Maalouf, Hengwei Zhu, Anika Zaman, Neena Carpino, Janet Hearing, Surita Bhatia, Isaac Sheridan Carrico","doi":"10.1002/cbic.202400988","DOIUrl":"https://doi.org/10.1002/cbic.202400988","url":null,"abstract":"Vaccines are a pivotal achievement in public health, offering inexpensive, distributable and highly effective protection against infectious diseases. Despite significant advancements in vaccine development, there are still many diseases for which vaccines are unavailable or offer limited protection. The global impact of the deficiency in vaccine-induced immunity against these diseases is profound, leading to increased rates of illness, more frequent hospitalizations, and higher mortality rates. Recent studies have demonstrated conjugation mechanisms and delivery methods to co-present adjuvants and protein epitopes to antigen-presenting cells, significantly enhancing adaptive immunity. We introduce a novel approach to incorporate an adjuvant into the vaccine by covalently attaching it to whole enveloped virions. Using \"clickable\" azide-enabled viral particles, generated through metabolic incorporation of N-azidoacetyl glucosamine (GlcNAz), we conjugated the virions with a cyclo-octyne-modified CpG-ODN. Conjugation yielded a potent adjuvant-virus complex, eliciting higher TLR9-mediated cell activation of cultured bone marrow-derived macrophages relative to co-administered adjuvants and virions. Administration of covalent adjuvant-virion conjugates increase immune cell stimulation and may provide a generalizable and effective strategy for eliciting a heightened immune response for vaccine development.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400988"},"PeriodicalIF":2.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cold-Adapted Lipid A from Polaribacter sp. SM1127: A Study of Structural Heterogeneity and Immunostimulatory properties.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-03-31 DOI: 10.1002/cbic.202500100

Roberta Cirella, Emanuela Andretta, Luca De Simone Carone, Francesca Olmeo, Mei-Ling Sun, Marcello Mercogliano, Yu-Zhong Zhang, Antonio Molinaro, Alba Silipo, Flaviana Di Lorenzo

{"title":"Cold-Adapted Lipid A from Polaribacter sp. SM1127: A Study of Structural Heterogeneity and Immunostimulatory properties.","authors":"Roberta Cirella, Emanuela Andretta, Luca De Simone Carone, Francesca Olmeo, Mei-Ling Sun, Marcello Mercogliano, Yu-Zhong Zhang, Antonio Molinaro, Alba Silipo, Flaviana Di Lorenzo","doi":"10.1002/cbic.202500100","DOIUrl":"https://doi.org/10.1002/cbic.202500100","url":null,"abstract":"Polaribacter sp. SM1127, a cold-adapted marine Gram-negative bacterium isolated from Laminaria in Arctic waters, plays a crucial role in nutrient cycling and biopolymer degradation in cold environments. Additionally, its exopolysaccharide (EPS) exhibits promising biotechnological potential, including antioxidant and wound-healing properties. This study focuses on the isolation and characterization of lipid A, the glycolipid component of Polaribacter sp. SM1127 lipopolysaccharide (LPS), by bypassing full LPS extraction and working directly with the ethanol precipitation product containing both EPS and bacterial cells. Mass spectrometry analysis revealed significant structural heterogeneity in the lipid A, with variations in fatty acid chain length, branching, saturation and hydroxylation. These features likely enable the bacterium to fine-tune its response to fluctuating temperatures or other cold-related environmental stresses, contributing to resilience in the Arctic Ocean ecosystem. Furthermore, immunological assays demonstrated that both LPS and EPS produced by Polaribacter sp. SM1127 induce weak TLR4 activation and, in general, poorly stimulate the NF-kB pathway, compared to E. coli LPS. These findings suggest their potential as immunomodulatory agents, like vaccine adjuvants.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500100"},"PeriodicalIF":2.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid Metabolism and Immune Function: Chemical Tools for Insights into T-Cell Biology.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-03-31 DOI: 10.1002/cbic.202400980

L Reinalda, M van der Stelt, Sander Izaak van Kasteren

{"title":"Lipid Metabolism and Immune Function: Chemical Tools for Insights into T-Cell Biology.","authors":"L Reinalda, M van der Stelt, Sander Izaak van Kasteren","doi":"10.1002/cbic.202400980","DOIUrl":"https://doi.org/10.1002/cbic.202400980","url":null,"abstract":"Lipids are essential biomolecules playing critical roles in cellular processes, including energy storage, membrane structure, and signaling. This review highlights the chemical tools that have been developed to study the role of lipid metabolism in immune function, focusing on T-cell biology. Fatty acids (FAs), as core lipid components, influence immune responses through structural, signaling, and metabolic roles. Recent studies reveal how specific FAs modulate T-cell activation, proliferation, and function, with implications for regulatory and effector subsets. Emerging tools, such as fluorescence-based lipids and click chemistry, enable precise tracking of lipid uptake and metabolism at single-cell levels, addressing limitations of traditional bulk methods. Advances in metabolomics and proteomics offer further insights into lipid-mediated immune regulation. Understanding these mechanisms provides opportunities for targeting lipid metabolism in therapeutic strategies for cancer and other immune-related diseases. The integration of lipidomic technologies into immunology uncovers novel perspectives on how lipids shape immune responses at cellular and molecular scales.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400980"},"PeriodicalIF":2.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning Tetrazine Substituents to Enhance Vinyltetrazine Labeling Performance.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-03-31 DOI: 10.1002/cbic.202500067

Haoxing Wu, Xinxin Liang, Jie Li, Xirui Liu, Xinyu He, Tingyu Chen, Shiyi Zhou

引用次数: 0