Cangsong Liao, David Lim, Gladwin Suryatin Alim, Florian P Seebeck
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5-thiohistidine N-acetyltransferase from Proteiniphilum saccharofermentans.
Ovothiol A is a 5-thiohistidine derivative biosynthesized by a broad range of prokaryotic and eukaryotic organisms. Its redox-active mercaptoimidazole side chain is believed to protect cells from oxidative stress. The three enzymes that produce ovothiol A from histidine, cysteine, and S-adenosyl methionine have been identified and characterized. In contrast, no enzymes are known that produce other 5-thiohistidine derivatives. Here, we describe a small family of acetyl-coenzyme A-dependent transferases that produce N-acetyl-5-thiohistidine. The discovery of these enzymes from Proteiniphilum saccharofermentans and related Bacteroidota provides evidence that the 5-thiohistidine class may be structurally and functionally more diverse than previously thought.
期刊介绍:
ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).