ChemBioChem最新文献_第4页

A Rapid and Sensitive MicroPlate Assay (MPSA) Using an Alkyne-Modified CMP-Sialic Acid Donor to Evaluate Human Sialyltransferase Specificity. 使用炔基修饰的 CMP-唾液酸供体评估人类唾液基转移酶特异性的快速灵敏微板测定 (MPSA)。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-29 DOI: 10.1002/cbic.202400539

Kiamungongo Clairene Filipe, Sushmaa Dangudubiyyam, Cédric Lion, Mathieu Decloquement, Roxana Elin Teppa, Christophe Biot, Anne Harduin-Lepers

引用次数: 0

Marker-dependent cleavage of RNA by binary (split) DNAzyme (BiDz) and binary DNA machines (biDNM). 二元（分裂）DNA 酶（BiDz）和二元 DNA 机（biDNM）对 RNA 的标记依赖性裂解。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-29 DOI: 10.1002/cbic.202400665

Mikhail V Dubovichenko, Daria D Nedorezova, Christina Patra, Valeria S Drozd, Vladimir S Andrianov, Anna I Ashmarova, Vivian O Nnanyereugo, Ahmed A Eldeeb, Dmitry M Kolpashchikov

{"title":"Marker-dependent cleavage of RNA by binary (split) DNAzyme (BiDz) and binary DNA machines (biDNM).","authors":"Mikhail V Dubovichenko, Daria D Nedorezova, Christina Patra, Valeria S Drozd, Vladimir S Andrianov, Anna I Ashmarova, Vivian O Nnanyereugo, Ahmed A Eldeeb, Dmitry M Kolpashchikov","doi":"10.1002/cbic.202400665","DOIUrl":"https://doi.org/10.1002/cbic.202400665","url":null,"abstract":"Oligonucleotide gene therapy (OGT) can be used to suppress specific RNA in cells and thus have been explored for gene therapy. Despite extensive effort, there is no clinically significant OGT for treating cancer. Low efficiency of OGT is one of the problems. Earlier, we proposed to address this problem by suppressing most vital genes in cancer cells e.g. housekeeping genes. To achieve specific activation of the OGT agents in cancer but not in normal cells, we designed binary (split) DNAzyme (BiDz), which is activated by cancer-related nucleic acid sequences. This work is devoted to BiDz optimization using cancer marker-related sequence as an activator and three folded RNA targets. To achieve efficient binding of folded RNA, the BiDz design was equipped with RNA binding/unwinding arms, a construction that was dabbed 'BiDz machines' (BiDM). BiDM was designed to have improved both iRNA cleavage rates and RNA recognition in comparison with BiDz. Further development of DNA nanotechnology-inspired agents can advance OGT technology in treating cancer, viral infections, and genetic disorders.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Universal Support for the Solid-Phase Synthesis of Peptidyl-tRNA Mimics. 肽基-tRNA 模拟物固相合成的通用支持。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-28 DOI: 10.1002/cbic.202400717

Julia Thaler, Christoph Mitteregger, Laurin Flemmich, Ronald Micura

{"title":"A Universal Support for the Solid-Phase Synthesis of Peptidyl-tRNA Mimics.","authors":"Julia Thaler, Christoph Mitteregger, Laurin Flemmich, Ronald Micura","doi":"10.1002/cbic.202400717","DOIUrl":"https://doi.org/10.1002/cbic.202400717","url":null,"abstract":"Hydrolysis-resistant RNA-peptide conjugates that mimic peptidyl-tRNAs are often required for structural and functional studies of protein synthesis at the ribosome. These conjugates can be synthesized by solid-phase chemical synthesis, which allows maximum flexibility in both the peptide and RNA sequence. The commonly used strategy is based on (3'-N-aminoacyl)-3'-amino-3'-deoxyadenosine solid supports, which already contain the first C-terminal amino acid of the target peptidyl chain. This is a limitation in the sense that different individual supports must be synthesized for different C-terminal amino acids. In this study, we demonstrate a solution to this problem by introducing a novel universal support. The key is a free ribose 3'-NH2 group that can be coupled to any amino acid. This is made possible by a photocleavable ether moiety that links the ribose 2'-O to the support, thus avoiding the typical O-to-N migration that occurs when using 2'-O-acyl linked solid supports. Once assembled, the conjugate is readily cleaved by UV irradiation. The structural integrity of the obtained peptidyl-RNA conjugates was verified by mass spectrometry analysis. In conclusion, the new photocleavable solid support makes the synthesis of 3'-peptidyl tRNA mimics of different peptidyl chains significantly more efficient compared to the commonly used approaches.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rewiring Lysine Catabolism in Cancer Leads to Increased Histone Crotonylation and Immune Escape. 癌症中赖氨酸分解代谢的重构导致组蛋白质粒化和免疫逃逸的增加

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-27 DOI: 10.1002/cbic.202400638

Kosta Besermenji, Rita Petracca

引用次数: 0

Supramolecular Conductive Hydrogels for Tissue Engineering Applications. 用于组织工程应用的超分子导电水凝胶。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-27 DOI: 10.1002/cbic.202400733

Aashwini Bhavsar, Falguni Pati, Priyadarshi Chakraborty

{"title":"Supramolecular Conductive Hydrogels for Tissue Engineering Applications.","authors":"Aashwini Bhavsar, Falguni Pati, Priyadarshi Chakraborty","doi":"10.1002/cbic.202400733","DOIUrl":"https://doi.org/10.1002/cbic.202400733","url":null,"abstract":"Owing to their unique attributes, including reversibility, specificity, directionality, and tunability, supramolecular biomaterials have evolved as an excellent alternative to conventional biomaterials like polymers, ceramics, and metals. Supramolecular hydrogels, in particular, have garnered significant interest because their fibrous architecture, high water content, and interconnected 3D network resemble the extracellular matrix to some extent. Consequently, supramolecular hydrogels have been used to develop biomaterials for tissue engineering. Supramolecular conductive hydrogels combine the advantages of supramolecular soft materials with the electrical properties of metals, making them highly relevant for electrogenic tissue engineering. Given the versatile applications of these hydrogels, it is essential to periodically review high-quality research in this area. In this review, we focus on recent advances in supramolecular conductive hydrogels, particularly their applications in tissue engineering. We discuss the conductive components of these hydrogels and highlight notable reports on their use in cardiac, skin, and neural tissue engineering. Additionally, we outline potential future developments in this field.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cracking Lysine Crotonylation (Kcr): Enlightening a Promising Post-Translational Modification. 破解赖氨酸巴豆酰化（Kcr）：揭示一种前景广阔的翻译后修饰。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-27 DOI: 10.1002/cbic.202400639

Marinda Westerveld, Kosta Besermenji, David Aidukas, Nikita Ostrovitsa, Rita Petracca

{"title":"Cracking Lysine Crotonylation (Kcr): Enlightening a Promising Post-Translational Modification.","authors":"Marinda Westerveld, Kosta Besermenji, David Aidukas, Nikita Ostrovitsa, Rita Petracca","doi":"10.1002/cbic.202400639","DOIUrl":"https://doi.org/10.1002/cbic.202400639","url":null,"abstract":"Lysine crotonylation (Kcr) is a recently discovered post-translational modification (PTM). Both histone and non-histone Kcr-proteins have been associated with numerous diseases including cancer, acute kidney injury, HIV latency, and cardiovascular disease. Histone Kcr enhances gene expression to a larger extend than the extensively studied lysine acetylation (Kac), suggesting Kcr as a novel potential therapeutic target. Although numerous scientific reports on crotonylation were published in the last years, relevant knowledge gaps concerning this PTM and its regulation still remain. To date, only few selective Kcr-interacting proteins have been identified and selective methods for the enrichment of Kcr-proteins in chemical proteomics analysis are still lacking. The development of new techniques to study this underexplored PTM could then clarify its function in health and disease and hopefully accelerate the development of new therapeutics for Kcr-related disease. Herein we briefly review what is known about the regulation mechanisms of Kcr and the current methods used to identify Kcr-proteins and their interacting partners. This report aims to highlight the significant potential of Kcr as a therapeutic target and to identify the existing scientific gaps that new research must address.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Potency and Specificity of Cryptophycin-Loaded Antibody-Drug Conjugates. 评估隐球菌素负载抗体-药物共轭物的效力和特异性

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-27 DOI: 10.1002/cbic.202400738

Peter Bitsch, Cedric Dessin, Sebastian Bitsch, Jona Voss, Janine Becker, Panna Sharma, Neha Biyani, Harry Kochat, Norbert Sewald, Harald Kolmar

引用次数: 0

(+)-3,6-Epoxymaaliane: A Novel Derivative of (+)-Bicyclogermacrene Oxidation Catalyzed by CYP450 BM3-139-3 and Its Variants. (+)-3,6-环氧马来酰亚胺：一种由 CYP450 BM3-139-3 及其变体催化的 (+)-Bicyclogermacrene 氧化作用的新型衍生物。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-27 DOI: 10.1002/cbic.202400410

Kai Xu, Zheng-Yu Huang, Chen-Yi Sun, Jiang Pan, Chun-Xiu Li, Jian-He Xu

{"title":"(+)-3,6-Epoxymaaliane: A Novel Derivative of (+)-Bicyclogermacrene Oxidation Catalyzed by CYP450 BM3-139-3 and Its Variants.","authors":"Kai Xu, Zheng-Yu Huang, Chen-Yi Sun, Jiang Pan, Chun-Xiu Li, Jian-He Xu","doi":"10.1002/cbic.202400410","DOIUrl":"https://doi.org/10.1002/cbic.202400410","url":null,"abstract":"(+)-Bicyclogermacrene is a sesquiterpene compound found in various plant essential oils and serves as a crucial precursor for multiple biologically active compounds. Many derivatives of (+)-bicyclogermacrene have been shown to exhibit valuable bioactivities. Cytochrome P450 BM3 from Bacillus megaterium can catalyze a variety of substrates and different types of oxidation reactions, making it become a powerful tool for oxidizing terpenes. In this study, we employed P450 BM3-139-3 variant for in vitro enzymatic oxidation of (+)-bicyclogermacrene, identifying a novel oxidized derivative of (+)-bicyclogermacrene, named (+)-3,6-epoxymaaliane, and an unknown sesquiterpenoid in a ratio of 70 : 30 (by GC peak area). (+)-3,6-Epoxymaaliane showed demonstrated antibacterial activities toward Escherichia coli and Staphylococcus aureus. To obtain a better variant of the monooxygenase with a high selectivity to form (+)-3,6-epoxymaaliane, we combined alanine scanning with the \"Focused Rational Iterative Site-Specific Mutagenesis\" (FRISM) strategy to modify the closest residues within 5 Å radius surrounding the substrate to create a small-but-smart library of mutants. Consequently, it gave an optimal variant with 1.6-fold improvement, in a turnover number (TON) of up to 964 toward (+)-3,6-epoxymaaliane production with a higher product selectivity.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferrocenyl β-Diketonate Compounds: Extended Ring Systems for Improved Anticancer Activity. 二茂铁基 β-二酮化合物：用于提高抗癌活性的扩展环系统。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-24 DOI: 10.1002/cbic.202400759

Benjamin J Hofmann, Enas T Aljohani, Natalia Cicovacki, Ivan Lee, Derek T Warren, Anastasia Sobolewski, Tameryn Stringer, Rianne M Lord

{"title":"Ferrocenyl β-Diketonate Compounds: Extended Ring Systems for Improved Anticancer Activity.","authors":"Benjamin J Hofmann, Enas T Aljohani, Natalia Cicovacki, Ivan Lee, Derek T Warren, Anastasia Sobolewski, Tameryn Stringer, Rianne M Lord","doi":"10.1002/cbic.202400759","DOIUrl":"https://doi.org/10.1002/cbic.202400759","url":null,"abstract":"A library of ferrocenyl β-diketonate compounds with varying degrees of aromatic functionality have been synthesized and fully characterized. This includes cyclic voltammetry and the analysis of four new structures by single crystal X-ray diffraction. The compounds cytotoxic potential has been determined by MTT screening against pancreatic carcinoma (MIA PaCa-2), ovarian adenocarcinoma (A2780), breast adenocarcinomas (MDA-MB-231 and MCF-7) and normal epithelial retinal (ARPE-19). The compounds show a general trend, where increasing the number of aromatic rings in the molecule yields an increase in cytotoxicity and follows the trend anthracenyl > naphthyl > phenyl > methyl. The compounds are particularly sensitive to the triple negative cancer cell line MDA-MB-231, and the potential modes of action have been studied by production of reactive oxygen species using fluorescence microscopy and cell morphology using Scanning Electron Microscopy. All assays highlight the ferrocenyl β-diketonate with an anthracenyl substituent to be the lead compound in this library. The decomposition was also observed within cells, to give a cytotoxic fluorescent molecule, which has been visualized by confocal microscopy.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light-driven Lytic Polysaccharide Monooxygenase Catalysis Mediated by Type I Photosensitizers. 由 I 型光敏剂介导的光驱动溶解多糖单氧化酶催化。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-24 DOI: 10.1002/cbic.202400486

Ana Gabriela Veiga Sepulchro, Milena Moreira Vacilotto, Lucas D Dias, Vanessa O A Pellegrini, Josman Velasco, Natalia M Inada, Fernando Segato, Igor Polikarpov

{"title":"Light-driven Lytic Polysaccharide Monooxygenase Catalysis Mediated by Type I Photosensitizers.","authors":"Ana Gabriela Veiga Sepulchro, Milena Moreira Vacilotto, Lucas D Dias, Vanessa O A Pellegrini, Josman Velasco, Natalia M Inada, Fernando Segato, Igor Polikarpov","doi":"10.1002/cbic.202400486","DOIUrl":"10.1002/cbic.202400486","url":null,"abstract":"The use of light as abundant, renewable, and clean energy source to boost lytic polysaccharide monooxygenase (LPMO) reactions represents an exciting and yet under-explored opportunity. Herein we demonstrated that photosensitizers, commonly used in photodynamic therapy, which act through the photocatalytic Type I mechanism can drive the oxidation of PASC by LPMOs, whereas Type II photosensitizers are not capable of promoting the LPMO activity. We analyzed Type I and Type II photosensitizers (methylene blue and tetraiodide salt of meso-tetrakis-(4-N-methylpyridyl) porphyrin, respectively) and demonstrated that, even without an addition of external reductant, Type I was capable of boosting Thermothelomyces thermophila MtLPMO9A activity in the presence of light. We also evaluated the photobiosystem in the presence and/or absence of molecular oxygen (O2) and hydrogen peroxide (H2O2), and investigated the role of superoxide radical in the methylene blue fueled reactions. Furthermore, we demonstrated that sodium bisulfite (NaHSO3), a chemical scavenger of H2O2, acts by safeguarding the enzyme from oxidative damage caused by accumulation of H2O2 early in photosensitizer-driven LPMO reactions. Finally, the results of the present work demonstrated that light-driven LPMO reactions mediated photodynamic therapy (PDT) Type I photosensitizers, which also includes molecules such as curcumin and riboflavin, is a general phenomenon.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0