Hongling Zhou, Yuanyuan Li, Jingwen Zhang, Li Wang, Ya Ying Zheng, Chengze Wu, Thomas J Begley, Jia Sheng, Rui Wang
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引用次数: 0
Abstract
RNA modifications play pivotal roles in numerous cellular processes and human diseases. In addition to the well-studied methyl-based modifications, hydrophobic prenyl-modifications have also been found in many RNA species. Herein, a chemical labeling strategy for tagging specific tRNAs by taking advantage of a natural SelU-mediated geranylation process is reported. A series of "clickable" geranyl-pyrophosphate analogs is synthesized and two candidates to label 2-thiouridine (s2U) containing tRNA using a two-step process, azidation-and-click-tagging with fluorescent dyes, namely ACT-Flu are identified. This method has also been applied to in-cell RNA imaging, providing a useful biochemical toolset to further study the biological functions of s2U-tRNAs and monitor their status in both healthy and diseased cells, since human tRNAs do not have the prenyl group on natural s2U34 modification.
期刊介绍：
ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).
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