ChemBioChem最新文献_第10页

d-Alanine, a Circadian Metabolite that Regulates Glucose Metabolism and Viral Infection.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-18 DOI: 10.1002/cbic.202500018

Tomonori Kimura, Shinsuke Sakai, Yoshitaka Isaka

引用次数: 0

Linker Ratio-Dependent Physicochemical Properties of ZIF-8 Encapsulated Mini Protein 20 Mimicking Uricase.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-17 DOI: 10.1002/cbic.202400832

Siti Fatimah Nur Abdul Aziz, Abu Bakar Salleh, Yahaya M Normi, Shahrul Ainliah Alang Ahmad

{"title":"Linker Ratio-Dependent Physicochemical Properties of ZIF-8 Encapsulated Mini Protein 20 Mimicking Uricase.","authors":"Siti Fatimah Nur Abdul Aziz, Abu Bakar Salleh, Yahaya M Normi, Shahrul Ainliah Alang Ahmad","doi":"10.1002/cbic.202400832","DOIUrl":"10.1002/cbic.202400832","url":null,"abstract":"Despite a growing interest in zeolitic imidazolate framework-8 (ZIF-8), notably for their potential as a host for various bio- and molecules, including peptides, the critical factors affecting their physicochemical properties and encapsulation efficiency are relatively unknown, limiting their widespread use. Herein, mini protein 20 (mp20), biomolecule mimicking uricase was used as a model to be hosted within ZIF-8 (mp20@ZIF-8) biocomposites. ZIF-8 were synthesized over a range of molar ratio of Zn to 2-methylimidazole(2-HmIm). By systematically exploring the impacts of various linker ratios, we found that a Zn to 2-HmIm ratio of 1 : 4 offers the highest encapsulation efficiency and thermal stability, making it particularly suitable for applications where these properties are critical. The 1 : 8 ratio, on the other hand, makes biocomposites with the most crystallized and a well-balanced combination of particle size and surface area, which are advantageous for applications requiring high structural integrity and surface interaction. This approach not only advances our understanding of protein encapsulation in MOFs but also provides new insights into how the linker-to-metal ratio can be optimized for different applications. Subsequent studies could expand upon these findings by implementing the enhanced biocomposites in practical applications, examining the encapsulation of biomolecules, or assessing the durability the long-term stability and functionality of these materials in various conditions.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400832"},"PeriodicalIF":2.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NMR Solution Structure of the N-Terminal GSPII Domain from the Thermus Thermophilus Traffic ATPase PilF and Reconstruction of its c-di-GMP Binding Capability.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-17 DOI: 10.1002/cbic.202400959

Konstantin Neißner, Carolin Frohnapfel, Heiko Keller, Elke Duchardt-Ferner, Vanessa Schneider, Zeinab Kamjou, Beate Averhoff, Jens Wöhnert

{"title":"NMR Solution Structure of the N-Terminal GSPII Domain from the Thermus Thermophilus Traffic ATPase PilF and Reconstruction of its c-di-GMP Binding Capability.","authors":"Konstantin Neißner, Carolin Frohnapfel, Heiko Keller, Elke Duchardt-Ferner, Vanessa Schneider, Zeinab Kamjou, Beate Averhoff, Jens Wöhnert","doi":"10.1002/cbic.202400959","DOIUrl":"10.1002/cbic.202400959","url":null,"abstract":"The cyclic dinucleotide c-di-GMP is an important second messenger molecule in bacteria and interacts with a variety of receptor molecules including RNA and protein domains. An important class of c-di-GMP-binding protein domains are the general secretory pathway type II (GSPII) domains as exemplified by the N-terminal domain of the ATPase MshE from Vibrio cholerae (MshEN). MshEN binds monomeric c-di-GMP via two consecutive copies of a 24-residue sequence motif, which form a compact 4-α-helical bundle. The ATPase PilF from Thermus thermophilus regulates pilus formation, motility and DNA-uptake. Its N-terminal section contains three consecutive GSPII domains (GSPII-A-GSPII-C) all with considerable sequence homology to MshEN. While the GSPII-B and the GSPII-C domains bind c-di-GMP, the GSPII-A domain does not. To determine why it is incapable of c-di-GMP-binding we determined the NMR-solution structure of this domain. Our structure shows how small deviations in the consensus motif sequence, a stabilizing N-terminal helical capping motif and intersubdomain interactions absent in MshEN cooperate to prevent c-di-GMP-binding. By combining point mutations and truncations, we re-established the c-di-GMP binding capability. Our findings shed new light on the evolution and functional diversification of GSPII domains and the importance of sequence variations for protein activity in this domain family.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400959"},"PeriodicalIF":2.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Study of DNA in Simulated Crowded Fluids.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-14 DOI: 10.1002/cbic.202500022

Mariam El-Morched, Jesse Vanloon, Frank Wien, Thad Harroun, Hongbin Yan

引用次数: 0

Front Cover: Self-Assembled BODIPY@Au Core-Shell Structures for Durable Neuroprotective Phototherapy (ChemBioChem 3/2025)

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-13 DOI: 10.1002/cbic.202580301

Ms. Melody Cai-Syaun Wu, Mr. Jack Hau-Ting Wei, Mr. Ricky Yu-Syun Fan, Ms. Eng Zhi Sim, Prof. Ken-Tye Yong, Prof. Tianxun Gong, Prof. Kien Voon Kong

引用次数: 0

Cover Feature: Understanding the P-Cluster of Vanadium Nitrogenase: an EPR and XAS Study of the Holo vs. Apo Forms of the Enzyme (ChemBioChem 3/2025) 封面专题：了解钒氮酶的 P 簇：酶的 Holo 与 Apo 形态的 EPR 和 XAS 研究（ChemBioChem 3/2025）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-13 DOI: 10.1002/cbic.202580302

Isis M. Wahl, Kushal Sengupta, Maurice van Gastel, Laure Decamps, Serena DeBeer

引用次数: 0

Effects of D-Amino Acid Replacements on the Conformational Stability of Miniproteins.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-13 DOI: 10.1002/cbic.202500085

Ruiwen Xu, Jiawen Huang, Ariel J Kuhn, Samuel H Gellman

引用次数: 0

Cover Feature: The Mysterious World of Non-Canonical Caps – What We Know and Why We Need New Sequencing Techniques (ChemBioChem 3/2025) 封面专题：非典型帽子的神秘世界--我们所知道的以及为什么我们需要新的测序技术（ChemBioChem 3/2025）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-13 DOI: 10.1002/cbic.202580304

Flaminia Mancini, Hana Cahova

引用次数: 0

Apparent Reversal of Allosteric Response in Mycobacterium tuberculosis MenD Reveals Links to Half-of-Sites Reactivity.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-13 DOI: 10.1002/cbic.202400943

Ngoc Anh Thu Ho, Fiona M Given, Tamsyn Stanborough, Michelle Klein, Timothy M Allison, Esther M M Bulloch, Wanting Jiao, Jodie M Johnston

{"title":"Apparent Reversal of Allosteric Response in Mycobacterium tuberculosis MenD Reveals Links to Half-of-Sites Reactivity.","authors":"Ngoc Anh Thu Ho, Fiona M Given, Tamsyn Stanborough, Michelle Klein, Timothy M Allison, Esther M M Bulloch, Wanting Jiao, Jodie M Johnston","doi":"10.1002/cbic.202400943","DOIUrl":"10.1002/cbic.202400943","url":null,"abstract":"Redox-active molecules play critical roles in various biological functions, including cellular respiration. In bacterial electron transport chains, menaquinones serve as key electron carriers. The first committed enzyme in the menaquinone biosynthesis pathway of Mycobacterium tuberculosis (Mtb), MenD, is allosterically inhibited by 1,4-dihydroxy-2-naphthoic acid (DHNA), the first redox-active metabolite in the pathway. Structural asymmetries in Mtb-MenD suggest that this enzyme operates via a half-of-sites mechanism for catalysis. Here, we investigate the interplay between its catalytic and allosteric mechanisms. Using molecular dynamics (MD) simulations, mutagenesis, kinetic and binding assays, and structural analyses, we identified and characterised mutants of two residues, D141 and D306, involved in stabilising asymmetric conformations associated with allostery. These mutations had complex effects on Mtb-MenD's reaction kinetics, with the D306 mutants showing an apparent reversal of the allosteric response to DHNA. Our findings indicate that asymmetric active site conformations may facilitate optimal binding of cofactors and substrates, while the transition between alternating active site conformations is essential for the catalytic cycle. DHNA binding stabilises asymmetry in the tetramer, likely promoting the binding of cofactors, substrates, or reaction intermediates. However, DHNA interferes with the transition between alternating conformations, ultimately impairing turnover and catalytic cycling in Mtb-MenD.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400943"},"PeriodicalIF":2.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and Emerging Strategies of Immunotherapy for Glioblastoma.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-13 DOI: 10.1002/cbic.202400848

Mingchuan Mao, Yao Lei, Xianbin Ma, Hai-Yan Xie

引用次数: 0