ChemBioChem最新文献_第10页

Small Molecule Fluorescent Probes for Glutathione S-Transferase. 谷胱甘肽s -转移酶的小分子荧光探针。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-19 DOI: 10.1002/cbic.202400994

Pingping Lu, Huiting Huang, Jia Liu, Yixuan Cao, Sheng Hua Liu, Jun Yin

引用次数: 0

Mitochondria-Targeted Temozolomide Probe for Overcoming MGMT-Mediated Resistance in Glioblastoma 线粒体靶向替莫唑胺探针克服mgmt介导的胶质母细胞瘤耐药。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-19 DOI: 10.1002/cbic.202400935

Daniel Szames, Dr. Shana O. Kelley

{"title":"Mitochondria-Targeted Temozolomide Probe for Overcoming MGMT-Mediated Resistance in Glioblastoma","authors":"Daniel Szames, Dr. Shana O. Kelley","doi":"10.1002/cbic.202400935","DOIUrl":"10.1002/cbic.202400935","url":null,"abstract":"Temozolomide (Tmz) is a DNA methylating agent used for the treatment of glioblastoma multiforme (GBM). Resistance to Tmz in GBM is caused by the DNA direct repair enzyme O6-methylguanine DNA methyltransferase (MGMT), which is expressed in ~50 % of GBM tumours. It has yet to be confirmed that MGMT acts within mitochondria to repair mitochondrial DNA (mtDNA), and in this report we discuss the development of a novel mitochondria-targeted temozolomide probe (mtTmz) for evading MGMT-mediated resistance. Through conjugation of Tmz to a mitochondria-penetrating peptide (MPP), exclusive mitochondrial localization was achieved, and the probe retained alkylation activity demonstrated by chemical and DNA-based assays. Absence of nuclear DNA damage was assessed by detecting γH2AX foci. mtTmz demonstrated efficient cell killing capabilities independent of MGMT status in GBM cells as determined by cell viability assays. It was determined using a Proteinase K digestion assay that MGMT does not translocate to mitochondria in response to mtTmz treatment, and RT-qPCR analysis demonstrated that mtTmz does not induce MGMT gene expression compared to Tmz. The results reported highlight both the potential of mitochondrial targeting of Tmz and mitochondria as a therapeutic target in MGMT-expressing GBM.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbic.202400935","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conjugation of Human N-Glycans Improves the Drug Properties of Existing Peptides and Proteins 人n -聚糖的偶联改善了现有多肽和蛋白质的药物特性。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-19 DOI: 10.1002/cbic.202401066

Dr. Yuji Nishiuchi, Dr. Sofia Elouali, Dr. Masato Noguchi, Dr. Hirofumi Ochiai

{"title":"Conjugation of Human N-Glycans Improves the Drug Properties of Existing Peptides and Proteins","authors":"Dr. Yuji Nishiuchi, Dr. Sofia Elouali, Dr. Masato Noguchi, Dr. Hirofumi Ochiai","doi":"10.1002/cbic.202401066","DOIUrl":"10.1002/cbic.202401066","url":null,"abstract":"Glycosylation is one of the most ubiquitous post-translational modifications observed in peptides and proteins. It affects the structural and functional characteristics of these macromolecules, thereby exerting a profound influence on a multitude of biological processes. N-Glycans are expected to be a beneficial modifier for increasing the solubility and in vivo half-life, and reducing the aggregation and immunogenicity of native bioactive peptides and proteins, which have seen limited clinical utility due to their short blood half-life and unsuitable physicochemical properties. Chemoselective glycosylation reactions that can be conducted post-synthesis and in aqueous conditions are a promising strategy for the high-throughput development of peptide/protein drugs. This “glycoconjugation” approach is particularly advantageous in that manipulation of glycan protecting groups is not necessary, thereby allowing conjugation reactions to be carried out between target molecules and unprotected glycans. By providing a single glycosylation profile, i. e., glycan structure, number, and position, glycoconjugation not only allows the beneficial properties of N-glycans to be exploited, but also facilitates the investigation of N-glycan function.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wireframe DNA Origami Capable of Vertex-protruding Transformation. 能够顶点突出转换的线框DNA折纸。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-19 DOI: 10.1002/cbic.202401071

Yosuke Ochi, Wataru Kato, Yoichi Tsutsui, Yuki Gomibuchi, Daichi Tominaga, Keisuke Sakai, Takeshi Araki, Suzunosuke Yoshitake, Takuo Yasunaga, Yusuke V Morimoto, Kazuhiro Maeda, Junichi Taira, Yusuke Sato

{"title":"Wireframe DNA Origami Capable of Vertex-protruding Transformation.","authors":"Yosuke Ochi, Wataru Kato, Yoichi Tsutsui, Yuki Gomibuchi, Daichi Tominaga, Keisuke Sakai, Takeshi Araki, Suzunosuke Yoshitake, Takuo Yasunaga, Yusuke V Morimoto, Kazuhiro Maeda, Junichi Taira, Yusuke Sato","doi":"10.1002/cbic.202401071","DOIUrl":"10.1002/cbic.202401071","url":null,"abstract":"Regulating dynamic behavior of the designed molecular structures provides a foundation for the construction of functional molecular devices. DNA nanotechnology allows conformational changes in two-dimensional and three-dimensional DNA origami nanostructures by introducing flexibility between the faces of the structures. However, dynamic transformations in wireframe DNA origami, composed solely of vertices and edges, remain challenging due to vertex-specific flexibility. We report a wireframe DNA origami capable of vertex-protruding transformation between the open- and closed-form with eight protruding vertices. This reversible transformation is driven by DNA hybridization and a toehold-mediated strand displacement reaction. Spacer strands between vertices and edges were designed to introduce flexibility. Coarse-grained molecular dynamics simulations demonstrated that a longer spacer increases conformational flexibility and can achieve the narrow angles required for the vertex-protruding transformation. The experimental results showed the successful assembly of the open-form structure under optimized salt conditions, as visualized through transmission electron microscopy images. Furthermore, the transformation between the open- and closed-form structures was demonstrated by the sequential addition of signal strands. This vertex-protruding transformation mechanism will expand the design approach of dynamic DNA nanostructures and help develop functional molecular devices for artificial molecular systems.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202401071"},"PeriodicalIF":2.6,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Feature: Recombinant Production of The Cyclotide Kalata B1 by Conditional Split Inteins (ChemBioChem 4/2025) 封面特写：条件分裂蛋白重组生产环肽Kalata B1 （ChemBioChem 4/2025）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-18 DOI: 10.1002/cbic.202580404

Dr. Abdulkadir Yayci, Dr. Yen-Hua Huang, Dr. Peta J. Harvey, Prof. David J. Craik

引用次数: 0

Front Cover: HPPE Activates NRF2 Signaling by Liberating Heavy Metal Stores (ChemBioChem 4/2025) 封面：HPPE通过释放重金属储存激活NRF2信号（ChemBioChem 4/2025）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-18 DOI: 10.1002/cbic.202580401

Rebecca Freeman, Michael J. Bollong

引用次数: 0

Anti-Neoplastic Gallium-Based PROTAC for PDI Degradation Triggers Autophagy and Immunogenic Cell Death 用于PDI降解的抗肿瘤镓基PROTAC可引发自噬和免疫原性细胞死亡。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-18 DOI: 10.1002/cbic.202400900

Xin-Xin Peng, Xiang-Yu Tan, Hang Zhang, Song Gao, Jun-Long Zhang

引用次数: 0

Impact of DNA Nick on the Self-Assembly of Single-Tile-Based Polyhedra DNA缺口对单瓦片多面体自组装的影响。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-18 DOI: 10.1002/cbic.202400973

Lingjun Wan, Zhiyuan Zhu, Mengzhou Wei, Prof. Yulin Li

{"title":"Impact of DNA Nick on the Self-Assembly of Single-Tile-Based Polyhedra","authors":"Lingjun Wan, Zhiyuan Zhu, Mengzhou Wei, Prof. Yulin Li","doi":"10.1002/cbic.202400973","DOIUrl":"10.1002/cbic.202400973","url":null,"abstract":"Tile-based self-assembly is a simple yet highly efficient bottom-up nanofabrication strategy, which has been widely applied in many fields such as structural DNA nanotechnology. The self-assembly of DNA tile is determined by not only the sequence design, but also the existing nick in the tile, as it would influence the flexibility and the symmetry of the tile. Herein, we systematically investigated the impact of nick on the tile-based assembly of three-dimensional (3D) DNA polyhedra in various conditions. A series of tile concentrations, four different sticky ends, two annealing approaches, buffers with different metal cations, and different molar percentages of intact tiles, were all tested. It was found that the tile concentration and the valence of metal cations played significant roles in how DNA nick influenced the assembly of DNA polyhedra. Besides, when the molar percentage of intact tiles was higher than the threshold of about two-thirds, the impact of nick on the assembly of DNA polyhedra would not be obvious. Our work provides an insightful understanding of the role of nick in the fabrication of high-quality structures from single-tile-based assembly.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Feature: Synthesis of Substituted Acyclic and Cyclic N-Alkylhydrazines by Enzymatic Reductive Hydrazinations (ChemBioChem 4/2025) 封面专题：酶还原肼合成取代无环和环n -烷基肼（ChemBioChem 4/2025）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-18 DOI: 10.1002/cbic.202580402

Niels Borlinghaus, Donato Calabrese, Lars Lauterbach, Bettina M. Nestl

引用次数: 0

Beyond Phenolics: Alternative Substrates for Type III Copper Enzymes 超越酚类：III型铜酶的替代底物。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-18 DOI: 10.1002/cbic.202400982

Matthias Pretzler, Annette Rompel

{"title":"Beyond Phenolics: Alternative Substrates for Type III Copper Enzymes","authors":"Matthias Pretzler, Annette Rompel","doi":"10.1002/cbic.202400982","DOIUrl":"10.1002/cbic.202400982","url":null,"abstract":"The type III copper enzyme family of tyrosinases (TYRs) catalyzes the ortho-hydroxylation and oxidation of phenols as well as the two-electron oxidation of catechols to ortho-quinones. TYRs use copper ions as their tightly bound cofactors and utilize molecular oxygen as their cosubstrate. They are responsible for physiologically important reactions like the formation of melanin, the primary pigment animals apply for protection against UV light. While the reactivity of TYRs on substrates containing aromatic hydroxy groups (i. e. phenols) is well recognized, reports clearly demonstrating that TYRs are active on aromatic amines as well have gone largely unnoticed. In this perspective we aim to bring together the sparse data on non-phenolic TYR substrates to illustrate the potential of TYRs for the oxidation of aminophenols and anilines. The activity of TYRs on aromatic amines extends the substance classes amenable to biotechnological production with TYRs from catechols to N-phenyl imines and phenoxazinone derivates and calls for the inclusion of TYRs among the candidates for oxidative modification of aromatic amines in metabolic pathways.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 7","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbic.202400982","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0