ChemBioChem最新文献_第9页

RETRACTION: High-Resolution Imaging of Human Cancer Proteins Using Microprocessor Materials. RETRACTION：使用微处理器材料对人类癌症蛋白质进行高分辨率成像。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-04 DOI: 10.1002/cbic.202400798

{"title":"RETRACTION: High-Resolution Imaging of Human Cancer Proteins Using Microprocessor Materials.","authors":"","doi":"10.1002/cbic.202400798","DOIUrl":"https://doi.org/10.1002/cbic.202400798","url":null,"abstract":"Retraction: M. J. Solares, G. M. Jonaid, W. Y. Luqiu, S. Berry, J. Khadela, Y. Liang, M. C. Evans, K. J. Pridham, W. J. Dearnaley, Z. Sheng and D. F. Kelly, \"High-Resolution Imaging of Human Cancer Proteins Using Microprocessor Materials,\" ChemBioChem, 23, no. 17 (2022), e202200310, https://doi.org/10.1002/cbic.202200310. The above article, published online on 5 July 2022 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Ruben Ragg; and Wiley-VCH GmbH. The retraction has been agreed following a thorough peer review process conducted by the author's institution, Penn State. The editors' own independent investigation confirmed that density map for the p53 dimer does not align well with the model for Figures 2B and S3 A. Furthermore, it was found that the coordinate and map files associated with the article available in the Protein Data Bank [PDB accession codes 8F2I (p53 monomer), 8F2H (p53 dimer)] and Electron Microscopy Data Bank [EMDB accession codes EMD28817 (p53 monomer), EMD28816 (p53 dimer)] exhibit poor alignment throughout. In addition, the authors did not provide original research data, maps and models in question, to justify the findings. The editors' own independent investigation confirmed that the conclusions of this manuscript are not sufficiently supported. Author D. F. Kelly responded to our notice of retraction, but did not state their agreement nor disagreement. Author G. M. Jonaid agrees with the retraction. All other authors did not respond to our notice regarding the retraction.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400798"},"PeriodicalIF":2.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemoenzymatic Cyclization by Vanadium Chloroperoxidase for Synthesis of 4-Hydroxyisochroman-1-Ones. 利用氯过氧化钒酶的化学合成环化作用合成 4-羟基异苯并二氢吡喃-1-酮。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-03 DOI: 10.1002/cbic.202400697

Chisanu Krongyut, Nittaya Wiriya, Worakrit Saiyasombat, Kantapat Chansaenpak, Sineenat Sripattanakul, Anyanee Kamkaew, Rung-Yi Lai

引用次数: 0

Distinct Effects of SARS-CoV-2 Protein Segments on Structural Stability, Amyloidogenic Potential, and α-Synuclein Aggregation. SARS-CoV-2 蛋白段对结构稳定性、淀粉样蛋白生成潜能和 α-突触核蛋白聚集的不同影响

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-31 DOI: 10.1002/cbic.202400598

Vince St Dollente Mesias, Jianing Zhang, Hongni Zhu, Xin Dai, Jixi Li, Jinqing Huang

{"title":"Distinct Effects of SARS-CoV-2 Protein Segments on Structural Stability, Amyloidogenic Potential, and α-Synuclein Aggregation.","authors":"Vince St Dollente Mesias, Jianing Zhang, Hongni Zhu, Xin Dai, Jixi Li, Jinqing Huang","doi":"10.1002/cbic.202400598","DOIUrl":"10.1002/cbic.202400598","url":null,"abstract":"Amyloidosis is characterized by the abnormal accumulation of misfolded proteins, called amyloid fibrils, leading to diverse clinical manifestations. Recent studies on the amyloidogenesis of SARS-CoV-2 protein segments have raised concerns on their potential link to post-infection neurodegeneration, however, the mechanisms remain unclear. Herein, we investigated the structure, stability, and amyloidogenic propensity of a nine-residue segment (SK9) of the SARS-CoV-2 envelope protein and their impact on neuronal protein α-synuclein (αSyn) aggregation. Specifically, the amino acid sequence of the SK9 wildtype has been modified from a basic and positively charged peptide (SFYVYSRVK), to a nearly neutral and more hydrophobic peptide (SAAVASAVK, labelled as SK9 var1), and to an acidic and negatively charged peptide (SDAVANAVK, labelled as SK9 var2). Our findings reveal that the SK9 wildtype exhibited a pronounced amyloidogenic propensity due to its disordered and unstable nature, while the SK9 variants possessed more ordered and stable structures preventing the amyloid formation. Significantly, the SK9 wildtype demonstrated distinct effect on αSyn aggregation kinetics and aggregate morphology to facilitate the formation of αSyn aggregates with enhanced resistance against enzymatic degradation. This study highlights the potential of modifying short peptide sequences to fine-tune their properties, providing insights into understanding and regulating viral-induced amyloid aggregations.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400598"},"PeriodicalIF":2.6,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Rapid and Sensitive MicroPlate Assay (MPSA) Using an Alkyne-Modified CMP-Sialic Acid Donor to Evaluate Human Sialyltransferase Specificity. 使用炔基修饰的 CMP-唾液酸供体评估人类唾液基转移酶特异性的快速灵敏微板测定 (MPSA)。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-29 DOI: 10.1002/cbic.202400539

Kiamungongo Clairene Filipe, Sushmaa Dangudubiyyam, Cédric Lion, Mathieu Decloquement, Roxana Elin Teppa, Christophe Biot, Anne Harduin-Lepers

引用次数: 0

Marker-Dependent Cleavage of RNA by Binary (split) DNAzyme (BiDz) and Binary DNA Machines (BiDM). 二元（分裂）DNA 酶（BiDz）和二元 DNA 机（biDNM）对 RNA 的标记依赖性裂解。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-29 DOI: 10.1002/cbic.202400665

Mikhail V Dubovichenko, Daria D Nedorezova, Christina Patra, Valeria S Drozd, Vladimir S Andrianov, Anna I Ashmarova, Vivian O Nnanyereugo, Ahmed A Eldeeb, Dmitry M Kolpashchikov

{"title":"Marker-Dependent Cleavage of RNA by Binary (split) DNAzyme (BiDz) and Binary DNA Machines (BiDM).","authors":"Mikhail V Dubovichenko, Daria D Nedorezova, Christina Patra, Valeria S Drozd, Vladimir S Andrianov, Anna I Ashmarova, Vivian O Nnanyereugo, Ahmed A Eldeeb, Dmitry M Kolpashchikov","doi":"10.1002/cbic.202400665","DOIUrl":"10.1002/cbic.202400665","url":null,"abstract":"Oligonucleotide gene therapy (OGT) can be used to suppress specific RNA in cells and thus has been explored for gene therapy. Despite extensive effort, there is no clinically significant OGT for treating cancer. Low efficiency of OGT is one of the problems. Earlier, we proposed to address this problem by suppressing the most vital genes in cancer cells e. g. housekeeping genes. To achieve specific activation of the OGT agents in cancer but not in normal cells, we designed a binary (split) DNAzyme (BiDz) activated by cancer-related nucleic acid sequences. This work is devoted to BiDz optimization for efficient cleavage of structured RNA targets upon activation with a cancer marker-related sequence. To achieve efficient binding of folded RNA, the BiDz was equipped with RNA binding/unwinding arms to produce 'Binary DNA-nanomachines' (BiDM). It was demonstrated that BiDM can improve both the rate and selectivity of RNA cleavage in comparison with BiDz. For the best selectivity, single-nucleotide variations should be recognized by the strand detached from the common DNA scaffold of BiDM. Further development of DNA nanotechnology-inspired agents can advance OGT in treating cancer, viral infections, and genetic disorders.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400665"},"PeriodicalIF":2.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Universal Support for the Solid-Phase Synthesis of Peptidyl-tRNA Mimics. 肽基-tRNA 模拟物固相合成的通用支持。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-28 DOI: 10.1002/cbic.202400717

Julia Thaler, Christoph Mitteregger, Laurin Flemmich, Ronald Micura

{"title":"A Universal Support for the Solid-Phase Synthesis of Peptidyl-tRNA Mimics.","authors":"Julia Thaler, Christoph Mitteregger, Laurin Flemmich, Ronald Micura","doi":"10.1002/cbic.202400717","DOIUrl":"10.1002/cbic.202400717","url":null,"abstract":"Hydrolysis-resistant RNA-peptide conjugates that mimic peptidyl-tRNAs are often required for structural and functional studies of protein synthesis at the ribosome. These conjugates can be synthesized by solid-phase chemical synthesis, which allows maximum flexibility in both the peptide and RNA sequence. The commonly used strategy is based on (3'-N-aminoacyl)-3'-amino-3'-deoxyadenosine solid supports, which already contain the first C-terminal amino acid of the target peptidyl chain. This is a limitation in the sense that different individual supports must be synthesized for different C-terminal amino acids. In this study, we demonstrate a solution to this problem by introducing a novel universal support. The key is a free ribose 3'-NH2 group that can be coupled to any amino acid. This is made possible by a photocleavable ether moiety that links the ribose 2'-O to the support, thus avoiding the typical O-to-N migration that occurs when using 2'-O-acyl linked solid supports. Once assembled, the conjugate is readily cleaved by UV irradiation. The structural integrity of the obtained peptidyl-RNA conjugates was verified by mass spectrometry analysis. In conclusion, the new photocleavable solid support makes the synthesis of 3'-peptidyl tRNA mimics of different peptidyl chains significantly more efficient compared to the commonly used approaches.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400717"},"PeriodicalIF":2.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rewiring Lysine Catabolism in Cancer Leads to Increased Histone Crotonylation and Immune Escape. 癌症中赖氨酸分解代谢的重构导致组蛋白质粒化和免疫逃逸的增加

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-27 DOI: 10.1002/cbic.202400638

Kosta Besermenji, Rita Petracca

引用次数: 0

Supramolecular Conductive Hydrogels for Tissue Engineering Applications. 用于组织工程应用的超分子导电水凝胶。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-27 DOI: 10.1002/cbic.202400733

Aashwini Bhavsar, Falguni Pati, Priyadarshi Chakraborty

{"title":"Supramolecular Conductive Hydrogels for Tissue Engineering Applications.","authors":"Aashwini Bhavsar, Falguni Pati, Priyadarshi Chakraborty","doi":"10.1002/cbic.202400733","DOIUrl":"10.1002/cbic.202400733","url":null,"abstract":"Owing to their unique attributes, including reversibility, specificity, directionality, and tunability, supramolecular biomaterials have evolved as an excellent alternative to conventional biomaterials like polymers, ceramics, and metals. Supramolecular hydrogels, in particular, have garnered significant interest because their fibrous architecture, high water content, and interconnected 3D network resemble the extracellular matrix to some extent. Consequently, supramolecular hydrogels have been used to develop biomaterials for tissue engineering. Supramolecular conductive hydrogels combine the advantages of supramolecular soft materials with the electrical properties of metals, making them highly relevant for electrogenic tissue engineering. Given the versatile applications of these hydrogels, it is essential to periodically review high-quality research in this area. In this review, we focus on recent advances in supramolecular conductive hydrogels, particularly their applications in tissue engineering. We discuss the conductive components of these hydrogels and highlight notable reports on their use in cardiac, skin, and neural tissue engineering. Additionally, we outline potential future developments in this field.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400733"},"PeriodicalIF":2.6,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cracking Lysine Crotonylation (Kcr): Enlightening a Promising Post-Translational Modification. 破解赖氨酸巴豆酰化（Kcr）：揭示一种前景广阔的翻译后修饰。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-27 DOI: 10.1002/cbic.202400639

Marinda Westerveld, Kosta Besermenji, David Aidukas, Nikita Ostrovitsa, Rita Petracca

{"title":"Cracking Lysine Crotonylation (Kcr): Enlightening a Promising Post-Translational Modification.","authors":"Marinda Westerveld, Kosta Besermenji, David Aidukas, Nikita Ostrovitsa, Rita Petracca","doi":"10.1002/cbic.202400639","DOIUrl":"https://doi.org/10.1002/cbic.202400639","url":null,"abstract":"Lysine crotonylation (Kcr) is a recently discovered post-translational modification (PTM). Both histone and non-histone Kcr-proteins have been associated with numerous diseases including cancer, acute kidney injury, HIV latency, and cardiovascular disease. Histone Kcr enhances gene expression to a larger extend than the extensively studied lysine acetylation (Kac), suggesting Kcr as a novel potential therapeutic target. Although numerous scientific reports on crotonylation were published in the last years, relevant knowledge gaps concerning this PTM and its regulation still remain. To date, only few selective Kcr-interacting proteins have been identified and selective methods for the enrichment of Kcr-proteins in chemical proteomics analysis are still lacking. The development of new techniques to study this underexplored PTM could then clarify its function in health and disease and hopefully accelerate the development of new therapeutics for Kcr-related disease. Herein we briefly review what is known about the regulation mechanisms of Kcr and the current methods used to identify Kcr-proteins and their interacting partners. This report aims to highlight the significant potential of Kcr as a therapeutic target and to identify the existing scientific gaps that new research must address.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400639"},"PeriodicalIF":2.6,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Potency and Specificity of Cryptophycin-Loaded Antibody-Drug Conjugates. 评估隐球菌素负载抗体-药物共轭物的效力和特异性

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-27 DOI: 10.1002/cbic.202400738

Peter Bitsch, Cedric Dessin, Sebastian Bitsch, Jona Voss, Janine Becker, Panna Sharma, Neha Biyani, Harry Kochat, Norbert Sewald, Harald Kolmar

引用次数: 0