ChemBioChem最新文献_第9页

Phase Separation in Biological Systems: Implications for Disease Pathogenesis. 生化和生物系统中的相分离：对疾病发病机制的影响。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-03 DOI: 10.1002/cbic.202400883

Raj Dave, Kshipra Pandey, Ritu Patel, Raghu Solanki, Nidhi Gour, Dhiraj Bhatia

引用次数: 0

Induction and Manipulation of Biomolecular Condensates Through Spatially Heterogeneous Solution Conditions. 空间异质溶液条件下生物分子凝聚物的诱导与调控。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-03 DOI: 10.1002/cbic.202500044

Alexander K Buell

引用次数: 0

SAAP-148 Oligomerizes into a Hexamer Forming a Hydrophobic Inner Core. SAAP-148寡聚成六聚体，形成疏水内核。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-01 DOI: 10.1002/cbic.202500112

Aden Hodzic, Djenana Vejzovic, Altea Topciu, Kirill Kuhlmann, Raj Kumar, Maria Andrea Mroginski, Alejandra de Miguel, Pia Hofmann, Klaus Zangger, Markus Weingarth, Robert A Cordfunke, Jan W Drijfhout, Peter Nibbering, Michal Belicka, Karl Lohner, Nermina Malanovic

{"title":"SAAP-148 Oligomerizes into a Hexamer Forming a Hydrophobic Inner Core.","authors":"Aden Hodzic, Djenana Vejzovic, Altea Topciu, Kirill Kuhlmann, Raj Kumar, Maria Andrea Mroginski, Alejandra de Miguel, Pia Hofmann, Klaus Zangger, Markus Weingarth, Robert A Cordfunke, Jan W Drijfhout, Peter Nibbering, Michal Belicka, Karl Lohner, Nermina Malanovic","doi":"10.1002/cbic.202500112","DOIUrl":"10.1002/cbic.202500112","url":null,"abstract":"Human cathelicidin LL-37 derivative, the 24-mer SAAP-148, is highly effective in vitro in eradicating multidrug-resistant bacteria without inducing resistance. SAAP-148 has a high cationic charge (+11) and 46% hydrophobicity, which, once the peptide folds into an alpha helix, forms a wide hydrophobic face. This highly amphipathic nature facilitates on the one hand its insertion into the membrane's fatty acyl chain region and on the other hand it´s interaction with anionic membrane components, which aids in killing bacteria. However, the contributions of the secondary and quaternary structures have not been thoroughly investigated so far. To address this, we applied circular dichroism, NMR spectroscopy, X-ray scattering, AlphaFold 3 protein folding software, and molecular dynamics simulations. Our results reveal that SAAP-148 adopts a stable hexameric bundle composed of three parallel dimers, that together form a hydrophobic core of aromatic side chain residues. The hexameric structure is retained at the membrane interface, whereby, MD simulation studies indicated the formation of a fiber-like structure in the presence of anionic membranes. This certainly seems plausible, as oligomers are stabilized by aromatic residues, and the exposure of positively charged side chains on the surface likely facilitates the transition of the peptide into fibrils on anionic membranes.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500112"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Endogenous Sequences Interacting with METTL3/METTL14 RNA Methyltransferase. 与METTL3/METTL14 RNA甲基转移酶相互作用的内源性序列的鉴定。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-01 DOI: 10.1002/cbic.202500006

Tamaki Endoh, Yiwei Ling, Shujiro Okuda, Miki Imanishi

{"title":"Identification of Endogenous Sequences Interacting with METTL3/METTL14 RNA Methyltransferase.","authors":"Tamaki Endoh, Yiwei Ling, Shujiro Okuda, Miki Imanishi","doi":"10.1002/cbic.202500006","DOIUrl":"10.1002/cbic.202500006","url":null,"abstract":"N6-methyladenosine (m6A) is the most abundant RNA modification in mRNA and regulates various biological processes. The RNA-binding properties of m6A writer proteins play an important role in determining RNA modification sites. METTL3 and METTL14 form the core of the m6A writer complex, with METTL3 as the catalytic methyltransferase and METTL14 as the RNA-binding scaffold. Thus far, the comprehensive RNA-binding properties of METTL3/14 remain unknown. Using RNA-capturing microsphere particles (R-CAMPs), immobilizing RNA fragments derived from endogenous RNAs of human lung carcinoma cells, RNA fragments that interacted with the METTL3/14 methyltransferase domain are isolated. Bioinformatics analysis reveals that the pool of isolated sequences contains significantly more regions with the potential to form RNA G-quadruplexes (rG4s) than the randomly extracted RNA sequence pool and that the (GGA) repeat sequences are most enriched. Circular dichroism spectroscopy, gel mobility shift assays, and methylation experiments demonstrate that METTL3/14 binds to RNA sequences containing (GGA) repeats that form rG4 structure much stronger than RNAs that do not form rG4s. This study shows the potential of RNA G-quadruplex structures as a modulator of epitranscriptomic modification of m6As.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500006"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioapplications of Cell Membrane Engineering with DNA Nanotechnology. DNA纳米技术在细胞膜工程中的生物应用。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-01 DOI: 10.1002/cbic.202500066

Jingyi Zheng, Jian-Rong Zhang, Sai Bi, Qianying Zhang, Jun-Jie Zhu

{"title":"Bioapplications of Cell Membrane Engineering with DNA Nanotechnology.","authors":"Jingyi Zheng, Jian-Rong Zhang, Sai Bi, Qianying Zhang, Jun-Jie Zhu","doi":"10.1002/cbic.202500066","DOIUrl":"10.1002/cbic.202500066","url":null,"abstract":"Engineering the cell surface has emerged as a significant method for manipulating cell behavior and determining cell fate. Regulating the composition or structure of cell membranes has the potential to impact the essential roles they play in biointerfacing, signal transduction, and compartmentalization. This presents significant prospects for the advancement of cell-based biomedicine. DNA nanotechnology has emerged as a promising experimental toolbox for cell membrane engineering, owing to its high programmability and excellent biocompatibility. Nongenetic strategies based on DNA nanotechnology for programming cell membranes have seen rapid growth over the past decade, showing promising prospects for cell-based therapeutic diagnostics. In this review, the nongenetic-based strategies for the functionalization of cell membranes are first introduced. The biological applications of DNA nanotechnology in cell membrane engineering are also highlighted and summarized, including molecular sensing, modulation of cell membrane signaling pathways and intercellular interactions, and establishment of transmembrane channels. Finally, the challenges and prospects of DNA nanotechnology in cell membrane engineering are discussed.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500066"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile Conjugation Method of CpG-ODN Adjuvant to Intact Virions for Vaccine Development. CpG-ODN佐剂与完整病毒粒子的快速偶联方法用于疫苗研制。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-03-31 DOI: 10.1002/cbic.202400988

Alexandra Maalouf, Hengwei Zhu, Anika Zaman, Neena Carpino, Janet Hearing, Surita Bhatia, Isaac Sheridan Carrico

{"title":"Facile Conjugation Method of CpG-ODN Adjuvant to Intact Virions for Vaccine Development.","authors":"Alexandra Maalouf, Hengwei Zhu, Anika Zaman, Neena Carpino, Janet Hearing, Surita Bhatia, Isaac Sheridan Carrico","doi":"10.1002/cbic.202400988","DOIUrl":"https://doi.org/10.1002/cbic.202400988","url":null,"abstract":"Vaccines are a pivotal achievement in public health, offering inexpensive, distributable and highly effective protection against infectious diseases. Despite significant advancements in vaccine development, there are still many diseases for which vaccines are unavailable or offer limited protection. The global impact of the deficiency in vaccine-induced immunity against these diseases is profound, leading to increased rates of illness, more frequent hospitalizations, and higher mortality rates. Recent studies have demonstrated conjugation mechanisms and delivery methods to co-present adjuvants and protein epitopes to antigen-presenting cells, significantly enhancing adaptive immunity. We introduce a novel approach to incorporate an adjuvant into the vaccine by covalently attaching it to whole enveloped virions. Using \"clickable\" azide-enabled viral particles, generated through metabolic incorporation of N-azidoacetyl glucosamine (GlcNAz), we conjugated the virions with a cyclo-octyne-modified CpG-ODN. Conjugation yielded a potent adjuvant-virus complex, eliciting higher TLR9-mediated cell activation of cultured bone marrow-derived macrophages relative to co-administered adjuvants and virions. Administration of covalent adjuvant-virion conjugates increase immune cell stimulation and may provide a generalizable and effective strategy for eliciting a heightened immune response for vaccine development.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400988"},"PeriodicalIF":2.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cold-Adapted Lipid A from Polaribacter sp. SM1127: A Study of Structural Heterogeneity and Immunostimulatory Properties. 来自极化杆菌sp. SM1127的冷适应脂质A：结构异质性和免疫刺激特性的研究。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-03-31 DOI: 10.1002/cbic.202500100

Roberta Cirella, Emanuela Andretta, Luca De Simone Carone, Francesca Olmeo, Mei-Ling Sun, Yu-Zhong Zhang, Marcello Mercogliano, Antonio Molinaro, Alba Silipo, Flaviana Di Lorenzo

{"title":"Cold-Adapted Lipid A from Polaribacter sp. SM1127: A Study of Structural Heterogeneity and Immunostimulatory Properties.","authors":"Roberta Cirella, Emanuela Andretta, Luca De Simone Carone, Francesca Olmeo, Mei-Ling Sun, Yu-Zhong Zhang, Marcello Mercogliano, Antonio Molinaro, Alba Silipo, Flaviana Di Lorenzo","doi":"10.1002/cbic.202500100","DOIUrl":"10.1002/cbic.202500100","url":null,"abstract":"Polaribacter sp. SM1127, a cold-adapted marine Gram-negative bacterium isolated from Laminaria in Arctic waters, plays a crucial role in nutrient cycling and biopolymer degradation in cold environments. Additionally, its exopolysaccharide (EPS) exhibits promising biotechnological potential, including antioxidant and wound-healing properties. This study focuses on the isolation and characterization of lipid A, the glycolipid component of Polaribacter sp. SM1127 lipopolysaccharide (LPS), by bypassing full LPS extraction and working directly with the ethanol precipitation product containing both EPS and bacterial cells. Mass spectrometry analysis reveals significant structural heterogeneity in the lipid A, with variations in fatty acid chain length, branching, saturation, and hydroxylation. These features likely enable the bacterium to fine-tune its response to fluctuating temperatures or other cold-related environmental stresses, contributing to resilience in the Arctic Ocean ecosystem. Furthermore, immunological assays demonstrate that both LPS and EPS produced by Polaribacter sp. SM1127 induce weak Toll-like receptor 4 activation and, in general, poorly stimulate the nuclear factor kappa-light-chain-enhancer of activated B cells pathway, compared to Escherichia coli LPS. These findings suggest their potential as immunomodulatory agents, like vaccine adjuvants.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500100"},"PeriodicalIF":2.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid Metabolism and Immune Function: Chemical Tools for Insights into T-Cell Biology. 脂质代谢和免疫功能：洞察t细胞生物学的化学工具。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-03-31 DOI: 10.1002/cbic.202400980

Luuk Reinalda, Mario van der Stelt, Sander Izaak van Kasteren

{"title":"Lipid Metabolism and Immune Function: Chemical Tools for Insights into T-Cell Biology.","authors":"Luuk Reinalda, Mario van der Stelt, Sander Izaak van Kasteren","doi":"10.1002/cbic.202400980","DOIUrl":"10.1002/cbic.202400980","url":null,"abstract":"Lipids are essential biomolecules playing critical roles in cellular processes, including energy storage, membrane structure, and signaling. This review highlights the chemical tools that have been developed to study the role of lipid metabolism in immune function, focusing on T-cell biology. Fatty acids (FAs), as core lipid components, influence immune responses through structural, signaling, and metabolic roles. Recent studies reveal how specific FAs modulate T-cell activation, proliferation, and function, with implications for regulatory and effector subsets. Emerging tools, such as fluorescence-based lipids and click chemistry, enable precise tracking of lipid uptake and metabolism at the single-cell level, addressing limitations of traditional bulk methods. Advances in metabolomics and proteomics offer further insights into lipid-mediated immune regulation. Understanding these mechanisms provides opportunities to target lipid metabolism in therapeutic strategies for cancer and other immune-related diseases. The integration of lipidomic technologies into immunology uncovers novel perspectives on how lipids shape immune responses at cellular and molecular scales.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2400980"},"PeriodicalIF":2.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning Tetrazine Substituents to Enhance Vinyltetrazine Labeling Performance. 调整四氮取代基以提高乙烯基四氮标记性能。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-03-31 DOI: 10.1002/cbic.202500067

Xinxin Liang, Jie Li, Xinyu He, Xirui Liu, Tingyu Chen, Shiyi Zhou, Haoxing Wu

引用次数: 0

Direct Preparation of Site-Specific Antibody-Drug Conjugates with Unpurified Antibodies in Culture Medium. 用未纯化抗体在培养基中直接制备位点特异性adc。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-03-28 DOI: 10.1002/cbic.202401082

Mengru Lu, Yue Zeng, Jiaying Hang, Wei Shi, Wei Huang, Feng Tang

{"title":"Direct Preparation of Site-Specific Antibody-Drug Conjugates with Unpurified Antibodies in Culture Medium.","authors":"Mengru Lu, Yue Zeng, Jiaying Hang, Wei Shi, Wei Huang, Feng Tang","doi":"10.1002/cbic.202401082","DOIUrl":"10.1002/cbic.202401082","url":null,"abstract":"In comparison with traditional chemotherapeutic drugs, antibody-drug conjugates (ADCs) have been extensively employed in cancer therapy due to their high selectivity and low systemic toxicity. However, the structural complexity of ADCs makes its preparation process more intricate and time-consuming. Conventional ADC production involves multistep purification processes, which significantly increase the costs. Herein, an approach for the direct preparation of site-specific ADCs in an antibody expression system that is derived from the reported K248 site-specific modification technology is demonstrated. This strategy requires only a single purification step to obtain site-specific ADCs, thereby greatly shortening the preparation cycle of ADCs. The preparation of site-specific ADCs in the expression system of Sacituzumab is successfully achieved. The resulting ADCs exhibited comparable physicochemical properties and activities to those obtained through the conventional two-step purification strategy. The approach demonstrates high compatibility with various linkers and antibodies, offering a streamlined methodology for the preparation of site-specific ADCs.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2401082"},"PeriodicalIF":2.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0