ChemBioChem最新文献_第8页

A Thermostable Fatty Acid Hydratase from Marinitoga Piezophila with Low Temperature Optimum and Broad Product Scope 一种低温最适产范围广的嗜热马里尼托加脂肪酸水合酶。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-26 DOI: 10.1002/cbic.202500081

Niels Krabbe Johnsen, David Marlo Werenberg Marcher, Mathias Bille Nielsen, Yan Zhang, Zheng Guo, Bekir Engin Eser

{"title":"A Thermostable Fatty Acid Hydratase from Marinitoga Piezophila with Low Temperature Optimum and Broad Product Scope","authors":"Niels Krabbe Johnsen, David Marlo Werenberg Marcher, Mathias Bille Nielsen, Yan Zhang, Zheng Guo, Bekir Engin Eser","doi":"10.1002/cbic.202500081","DOIUrl":"10.1002/cbic.202500081","url":null,"abstract":"Hydroxy fatty acids (HFAs) are valuable derivatives of fatty acids (FAs) with interesting bioactivities. Moreover, they are used in materials industry as additives, starting materials and surfactants. HFAs can be produced from FAs either by hydroxylation or by hydration reaction, if FA is unsaturated, using chemical or enzymatic methods. FA hydratases (FAHs) are promising biocatalysts for HFA synthesis thanks to their non-redox nature, high efficiency and excellent selectivity. Although FAHs are relatively more stable compared to other enzymes like monooxygenases, their tolerance to high temperature and organic solvents is limited. In this study, we characterized a rare thermostable FAH ortholog through database gene mining. This enzyme from Marinitoga Piezophila, a thermo-piezophilic organism, displayed novel properties, including broad substrate scope, broad pH range, unique regioselectivity and excellent thermostability (retaining full activity after 30 min incubation at 70 °C); however, quite interestingly, its temperature optimum was at 20 °C. Although kinetic parameters indicate a less efficient enzyme compared to some other FAHs, the enzyme can reach over 90% conversion within 24 h at a 100 mL scale reaction containing 1.75 mM substrate. Furthermore, mutagenesis of key active-site residues indicated a possibly different reaction mechanism compared to earlier proposed mechanisms.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 17","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12442217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aspartimide Formation and Its Prevention in Fmoc Chemistry Solid Phase Peptide Synthesis Fmoc化学固相肽合成中阿斯巴胺的形成及其预防。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-26 DOI: 10.1002/cbic.202500490

Marco J. W. Kong, Tim J. H. P. van den Braak, Kevin Neumann

引用次数: 0

Front Cover: Chromenone Derivatives as CRM1 Inhibitors for Targeting Glioblastoma (ChemBioChem 15/2025) 封面：染色质衍生物作为靶向胶质母细胞瘤的CRM1抑制剂（ChemBioChem 15/2025）

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-25 DOI: 10.1002/cbic.70013

Salvatore Princiotto, Lucía Jiménez, Lucía Domínguez, João G. N. Sequeira, Cristiana Mourato, Alba Orea-Soufi, Bruno Santos, Sabrina Dallavalle, Miguel Machuqueiro, Bibiana I. Ferreira, Wolfgang Link

引用次数: 0

Cover Feature: Characterization of the Cubamyces Menziesii Terpenome (ChemBioChem 15/2025) 封面专题：Cubamyces Menziesii Terpenome的表征（ChemBioChem 15/2025）

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-25 DOI: 10.1002/cbic.70014

Létitia Leydet, Julie Couillaud, Agnès Amouric, Elise Courvoisier-Dezord, Carole Avesque, Thierry Giardina, Mireille Attolini, Pierre Rousselot-Pailley, Katia Duquesne, Marie-Noelle Rosso, Gilles Iacazio

引用次数: 0

Light-Triggered Disassembly of Peptide Nanostructures 肽纳米结构的光触发分解。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-14 DOI: 10.1002/cbic.202500414

Raphael Meyer, Julian Link, Lucas Gunkel, Albin Lahu, Hakan Demirezen, Tanja Weil, David Y. W. Ng

{"title":"Light-Triggered Disassembly of Peptide Nanostructures","authors":"Raphael Meyer, Julian Link, Lucas Gunkel, Albin Lahu, Hakan Demirezen, Tanja Weil, David Y. W. Ng","doi":"10.1002/cbic.202500414","DOIUrl":"10.1002/cbic.202500414","url":null,"abstract":"While the assembly of supramolecular peptide nanostructures with diverse functions and morphologies has been extensively studied, the controlled disassembly of these architectures remains less understood. To address this, two short amphiphilic peptides incorporating anthracene as a light-responsive unit and lysine as a pH-sensitive residue are designed. These peptides self-assemble into nanosheets or nanoribbons with distinct secondary structures, which are further tunable by pH through modulation of peptide charge. Upon irradiation at 365 nm, the anthracene moieties undergo a bimolecular [4 + 4] cycloaddition, disrupting the π–π stacking interactions by distorting the planarity of the aromatic units. This photoreaction leads to disassembly of the supramolecular architectures within 10 min. Unlike monomolecular reactions such as photocleavage, the kinetics of this bimolecular photodimerization are significantly influenced by the degree of preorganization within the assemblies. These findings underscore the critical interplay between supramolecular architecture and molecular photochemistry, enabling light-triggered, structure-dependent disassembly of diverse peptide nanostructures.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 16","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/cbic.202500414","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Oligonucleotides Containing a Dna Distorting Interstrand Crosslink Produced by Mitomycins 丝裂霉素产生的含有Dna扭曲链交联的寡核苷酸的合成。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-14 DOI: 10.1002/cbic.202500221

Sarah Marks, Gabriel Martinez, Michael Adamov, Christopher Alley, Bianca Davila, Ana G. Petrovic, Rinat Abzalimov, Padmanava Pradhan, Marta Concheiro-Guisan, Elise Champeil

{"title":"Synthesis of Oligonucleotides Containing a Dna Distorting Interstrand Crosslink Produced by Mitomycins","authors":"Sarah Marks, Gabriel Martinez, Michael Adamov, Christopher Alley, Bianca Davila, Ana G. Petrovic, Rinat Abzalimov, Padmanava Pradhan, Marta Concheiro-Guisan, Elise Champeil","doi":"10.1002/cbic.202500221","DOIUrl":"10.1002/cbic.202500221","url":null,"abstract":"Mitomycin C (MC) is a powerful chemotherapy agent currently used in clinics for the treatment of various types of cancer. MC functions by inhibiting cellular growth through the formation of cytotoxic interstrand crosslinks (ICLs). These ICLs induced by MC have minimal impact on the DNA backbone, preserving its B-DNA structure. Recent research suggests that the cellular machinery recognizes and repairs ICLs differently based on their specific structure. To better understand how DNA distortion caused by MC ICLs influences cytotoxic effects, Herein, a novel mitomycin ICL is synthiesized that, unlike MC, significantly distorts DNA and widens the minor groove. This work outlines the synthesis of oligonucleotides bearing a single monoadduct or a single ICL of this new MC derivative at a defined position. Such substrates are widely used for investigations into biological processes such as DNA damage/repair studies. The monoadducted and crosslinked oligonucleotides are thoroughly characterized using various techniques, including enzymatic digestion to nucleosides, mass and circular dichroism spectroscopy, as well as thermal denaturation studies. Furthermore, the structure of this novel crosslinked duplex is compared with that of less-distorting mitomycin ICLs using Molecular Dynamics simulations.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 16","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of Fluidized Bed Reactors in Biocatalysis 流化床反应器在生物催化中的应用。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-14 DOI: 10.1002/cbic.202500143

Nadia Guajardo, Paula Grez, Ricardo S. Schrebler, Rodrigo A. Schrebler

{"title":"Applications of Fluidized Bed Reactors in Biocatalysis","authors":"Nadia Guajardo, Paula Grez, Ricardo S. Schrebler, Rodrigo A. Schrebler","doi":"10.1002/cbic.202500143","DOIUrl":"10.1002/cbic.202500143","url":null,"abstract":"The main objective of this article is to review previous contributions on the applications of fluidized bed reactors (FBR) in biocatalysis. FBR combines the properties of a stirred tank reactor and a continuous tubular reactor, making it an efficient system for carrying out enzymatic reactions with immobilized enzymes. This equipment's advantages include its high transfer capacity and versatility, as it can be used with liquid and gaseous phases. According to the literature, these devices have been primarily used to degrade contaminants, synthesize cosmetic ingredients, produce food and pharmaceutical compounds, and synthesize biolubricants and biodiesel. The enzymes most used in fluidized bed mode are laccases, lipases, and proteases immobilized on methacrylate resins, mesoporous silicas, alginate, and chitosan beads. Enzyme immobilization is essential, as it can promote the suspension of biocatalyst particles, thereby increasing yields and productivity. One of the leading prospects for these systems is to stabilize the fluidized bed using a magnetic field and the concept of “microfluidization,” which enables the stabilization of smaller biocatalyst particles with smaller equipment, thereby increasing efficiency and intensifying the biocatalytic process. In the future, the versatility of FBR will constitute an attractive alternative for developing biocatalytic systems.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Proteinosomes with Cellular-Like Functionalities 具有细胞样功能的工程蛋白体。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-14 DOI: 10.1002/cbic.202500448

Renzhuo Li, Xiaoman Liu, Xin Huang

{"title":"Engineering Proteinosomes with Cellular-Like Functionalities","authors":"Renzhuo Li, Xiaoman Liu, Xin Huang","doi":"10.1002/cbic.202500448","DOIUrl":"10.1002/cbic.202500448","url":null,"abstract":"Recent development of various types of functional microcompartmentalized ensembles that can imitate rudimentary aspects of living cells has attracted great attentions in a wide range of research communities. Proteinosome is one typical form of these biomimetic structures. Significant advances in their construction have indicated that by utilizing interfacial self-assembly, polymer-based membrane templating, and hybrid lipid–polymer systems can achieve tunable permeability, mechanical stability, and stimuli–responsive behaviors, which is detailedly elaborated in this review. Additionally, this review emphasizes their functionalization strategies that enable programable bioactivity and communication, as well as community aggregation structures to build prototissues exhibiting cellular-like behaviors. Innovations in stimuli–responsive materials and multicompartmentalized architectures further enhance spatiotemporal control over biochemical processes, while the convergence of polymer chemistry, synthetic biology, and nanotechnology continues to expand the functional scope of proteinosomes, positioning them as transformative tools for next-generation artificial cell models or biomimetic materials. Having covered these topics in depth, this review hopes to inspire additional investigation into the research methodologies related to proteinosomes, furthermore the artificial cells, as well as possible applications in targeted cancer therapy, gene therapy, and artificial organelles design.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 16","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diastereoselective, Diversifiable Synthesis and Biological Evaluation of the Virginiamycin Inducers, the Virginiae Butanolides 维吉尼亚霉素诱导剂维吉尼亚丁内酯的非对映选择性、多样化合成及生物学评价。

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-14 DOI: 10.1002/cbic.202500386

Kylie G. Castator, Manuela Frias-Gomez, Lauren E. Wilbanks, Elizabeth I. Parkinson

{"title":"Diastereoselective, Diversifiable Synthesis and Biological Evaluation of the Virginiamycin Inducers, the Virginiae Butanolides","authors":"Kylie G. Castator, Manuela Frias-Gomez, Lauren E. Wilbanks, Elizabeth I. Parkinson","doi":"10.1002/cbic.202500386","DOIUrl":"10.1002/cbic.202500386","url":null,"abstract":"Streptomyces species are renowned for their ability to produce bioactive natural products (NPs) via biosynthetic gene clusters (BGCs). However, many BGCs remain transcriptionally silent under standard laboratory conditions. Among the key regulatory mechanisms for NP biosynthesis are the γ-butyrolactone (GBL) signaling molecules, which have been widely studied for their role in repressor-molecule circuits. While the S. coelicolor butanolides (SCBs) and A-factor from S. griseus have been extensively studied, the virginiae butanolides (VBs) from S. virginiae, which alleviate repression of the biosynthesis of the antibiotic virginiamycins via binding to the cluster situated TetR-like repressor BarA, remain understudied. This is in large part due to limited access to enantiopure VBs. Herein, we report a diastereoselective and diversifiable route to access the VB hormones, starting from a protected (R)-paraconyl alcohol intermediate. A library of VB derivatives was synthesized and tested for their ability to alleviate repression of BarA using a newly developed green fluorescent protein (GFP) reporter assay. The synthesis and assay described herein established the most quantitative structure–activity relationship (SAR) analysis of the VBs to date. Overall, this study provides new tools for probing NP regulation in Streptomyces and enables new strategies for BGC activation using synthetic GBL molecules.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 17","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12442216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-Targeting of PD-L1 and Integrin αvβ3 for Preclinical PET Imaging of Cancer PD-L1和整合素αvβ3在肿瘤临床前PET成像中的双重靶向作用

IF 2.8 4区生物学

ChemBioChem Pub Date : 2025-08-14 DOI: 10.1002/cbic.202500508

Wenhao Liu, Xiushuang Yuan, Siqi Zhang, Xingkai Wang, Xin Gao, Hao Tian, Dun Wang, Ming-Rong Zhang, Rui Wang, Kuan Hu

{"title":"Dual-Targeting of PD-L1 and Integrin αvβ3 for Preclinical PET Imaging of Cancer","authors":"Wenhao Liu, Xiushuang Yuan, Siqi Zhang, Xingkai Wang, Xin Gao, Hao Tian, Dun Wang, Ming-Rong Zhang, Rui Wang, Kuan Hu","doi":"10.1002/cbic.202500508","DOIUrl":"10.1002/cbic.202500508","url":null,"abstract":"The dual-targeting strategy has demonstrated advantages in enhancing tumor uptake, improving imaging contrast, and ultimately increasing tumor detection rate. PD-L1 is overexpressed on multiple tumor cells and regulated by αvβ3-integrin. In this study, a dual-targeting radiotracer, [64Cu]-PEG-RGD-TPP-1, is developedfor PET/CT imaging of both PD-L1 and αvβ3-integrin simultaneously, achieving high contrast, enhanced tumor uptake, and prolonged tumor retention time. [64Cu]-PEG-RGD-TPP-1 comprises the peptide TPP-1 and cyclic peptide c(RGDyC), linked via a PEG linker. The dual-targeting molecule had a moderate serum stability (≈60%) in vivo after 1 hr. This dual-targeting radiotracer is evaluated and compared with the single-targeting radiotracers [64Cu]-PEG-TPP-1 and [64Cu]-TPP-1. PET imaging and ex vivo biodistribution studies show that [64Cu]-PEG-RGD-TPP-1 exhibits higher tumor uptake than its single-targeting counterparts. Moreover, the dual-targeting radiotracer demonstrated potential for ultrasmall tumor imaging and could be combined with X-ray irradiation to further enhance PET imaging contrast, thereby improving tumor-targeting efficiency. These findings suggest that [64Cu]-PEG-RGD-TPP-1 is a promising noninvasive tracer for detecting tumors expressing PD-L1 and/or integrin avβ3, with the prospect of clinical implementation.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0