ChemBioChem最新文献_第8页

Mitochondrial Viscosity Probes: Iridium(III) Complexes Induce Apoptosis in HeLa Cells. 线粒体粘度探针：铱（III）复合物诱导 HeLa 细胞凋亡。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-08 DOI: 10.1002/cbic.202400756

Bingbing Chen, Zhijun Liang, Yao Gong, Wei Wu, Jiaen Huang, Jiaxi Chen, Yanmei Wang, Jun Mei, Rui Chen, Zunnan Huang, Jing Sun

引用次数: 0

High-Throughput Discovery of Substrate Peptide Sequences for E3 Ubiquitin Ligases Using a cDNA Display Method. 利用 cDNA 显示法高通量发现 E3 泛素连接酶的底物多肽序列

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-07 DOI: 10.1002/cbic.202400617

Kenwa Tamagawa, Robert E Campbell, Takuya Terai

{"title":"High-Throughput Discovery of Substrate Peptide Sequences for E3 Ubiquitin Ligases Using a cDNA Display Method.","authors":"Kenwa Tamagawa, Robert E Campbell, Takuya Terai","doi":"10.1002/cbic.202400617","DOIUrl":"10.1002/cbic.202400617","url":null,"abstract":"Cells utilize ubiquitin as a posttranslational protein modifier to convey various signals such as proteasomal degradation. The dysfunction of ubiquitylation or following proteasomal degradation can give rise to the accumulation and aggregation of improperly ubiquitylated proteins, which is known to be a general causation of many neurodegenerative diseases. Thus, the characterization of substrate peptide sequences of E3 ligases is crucial in biological and pharmaceutical sciences. In this study, we developed a novel high-throughput screening system for substrate peptide sequences of E3 ligases using a cDNA display method, which enables covalent conjugation between peptide sequences and their corresponding cDNA sequences. First, we focused on the MDM2 E3 ligase and its known peptide substrate as a model to establish the screening method, and confirmed that cDNA display method was compatible with in vitro ubiquitylation. Then, we demonstrated identification of MDM2 substrate sequences from random libraries to identify a novel motif (VKFTGGQLA). Bioinformatics analysis of the hit sequences was performed to gain insight about endogenous substrate proteins.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400617"},"PeriodicalIF":2.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Insights into the Reaction Mechanisms of Non-Heme Diiron Enzymes Containing Oxoiron(IV) Complexes. 对含氧铁(IV)络合物的非半胱氨酸二铁酶反应机制的最新见解。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-07 DOI: 10.1002/cbic.202400788

Rui-Ning Li, Shi-Lu Chen

引用次数: 0

Front Cover: Uracil-Selective Cross-Linking in RNA and Inhibition of miRNA Function by 2-Amino-6-vinyl-7-deazapurine Deoxynucleosides (ChemBioChem 21/2024) 封面：2-氨基-6-乙烯基-7-脱氮嘌呤脱氧核苷在 RNA 中的尿嘧啶选择性交联和对 miRNA 功能的抑制（ChemBioChem 21/2024）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-06 DOI: 10.1002/cbic.202482101

Nadya Soemawisastra, Hidenori Okamura, Ahmed Mostafa Abdelhady, Kazumitsu Onizuka, Mamiko Ozawa, Fumi Nagatsugi

引用次数: 0

ER Oxidoreductin 1-Like Activity of Cyclic Diselenides Drives Protein Disulfide Isomerase in an Electron Relay System. 环状二硒化物的 ER 氧化还原蛋白 1 类活性在电子中继系统中驱动蛋白二硫异构酶。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-06 DOI: 10.1002/cbic.202400739

Rumi Mikami, Yuya Nishizawa, Yuki Iwata, Shingo Kanemura, Masaki Okumura, Kenta Arai

{"title":"ER Oxidoreductin 1-Like Activity of Cyclic Diselenides Drives Protein Disulfide Isomerase in an Electron Relay System.","authors":"Rumi Mikami, Yuya Nishizawa, Yuki Iwata, Shingo Kanemura, Masaki Okumura, Kenta Arai","doi":"10.1002/cbic.202400739","DOIUrl":"10.1002/cbic.202400739","url":null,"abstract":"Disulfide formation generally involves a two-electron oxidation reaction between cysteine residues. Additionally, disulfide formation is an essential post-translational modification for the structural maturation of proteins. This oxidative folding is precisely controlled by an electron relay network constructed by protein disulfide isomerase (PDI), with a CGHC sequence as the redox-active site, and its family enzymes. Creating reagents that mimic the functions of these enzymes facilitates folding during chemical protein synthesis. In this study, we aimed to imitate a biological electron relay system using cyclic diselenide compounds as surrogates for endoplasmic reticulum oxidoreductin 1 (Ero1), which is responsible for the re-oxidation of PDI. Oxidized PDI (PDIox) introduces disulfide bonds into substrate proteins, resulting in its conversion to reduced PDI (PDIred). The PDIred is then re-oxidized to PDIox by a coexisting cyclic diselenide compound, thereby restoring the function of PDI as a disulfide-forming agent. The produced diselenol state is readily oxidized to the original diselenide state with molecular oxygen, continuously sustaining the PDI catalytic cycle. This artificial electron relay system regulating enzymatic PDI function effectively promotes the oxidative folding of disulfide-containing proteins, such as insulin - a hypoglycemic formulation - by enhancing both yield and reaction velocity.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400739"},"PeriodicalIF":2.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein Condensates Unfold G-Quadruplex Resembling a Helicase Activity. 蛋白质缩聚物展开类似螺旋酶活性的 G 型四联体。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-06 DOI: 10.1002/cbic.202400791

Liang Luo, Shixia Ji, Qiong Wu, Guohua Xu, Jiajing Zhao, Yixiang Liu, Lang Chen, Maili Liu, Ling Jiang, Conggang Li

引用次数: 0

Cover Feature: Selective Dissolution of Calcium Pyrophosphate Dihydrate Crystals Using a Pyrophosphate Specific Receptor (ChemBioChem 21/2024) 封面专题：利用焦磷酸特异性受体选择性溶解二水焦磷酸钙晶体（ChemBioChem 21/2024）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-06 DOI: 10.1002/cbic.202482102

Zachary H. Paine, Mayank Sharma, Simon H. Friedman

引用次数: 0

Close Proximity of Cholesterol Anchors in Membrane Induces the Dissociation of Amphiphilic DNA Strand from Membrane Surface. 膜中胆固醇锚的接近会导致两亲性 DNA 链从膜表面解离

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-05 DOI: 10.1002/cbic.202400748

Canqiong Hu, Shuang Liu, Guoxia Huang, Fan Yang, Lexun Li, Cao Zhang, Shuxuan Shao, Xiaodan Deng, Qiaoling Liu

{"title":"Close Proximity of Cholesterol Anchors in Membrane Induces the Dissociation of Amphiphilic DNA Strand from Membrane Surface.","authors":"Canqiong Hu, Shuang Liu, Guoxia Huang, Fan Yang, Lexun Li, Cao Zhang, Shuxuan Shao, Xiaodan Deng, Qiaoling Liu","doi":"10.1002/cbic.202400748","DOIUrl":"10.1002/cbic.202400748","url":null,"abstract":"Dynamic DNA nanotechnology is appealing for membrane surface engineering due to their versatility and programmability. To modulate the dynamic interactions between the DNA functional units immobilized on membrane surface, membrane-anchored DNA functional units often come into close proximity each other due to DNA base pairing, which also leads to the close contact of the hydrophobic anchors in membrane. However, whether the close contact of hydrophobic anchors induces the dissociation of amphiphilic DNA structures from membrane surface is not concerned. Herein, we utilized cholesterol-labelled single-stranded DNA (ssDNA) as a simplified amphiphilic DNA structure to investigate the stability of membrane anchored DNA strands upon the closely contact of cholesterol anchors. The close contact of cholesterol-labelled ssDNA molecules driven by toehold mediated strand displacement reaction leads to approximately 41 % membrane anchored ssDNA dissociation from membrane surface, indicating the proximal cholesterol anchors in membrane could reduce the anchoring stability of cholesterol-modified DNA strands. This work enhances our understanding of the interactions between amphiphilic DNA and membranes, and provides valuable insights for the design of future DNA constructs intended for applications involving dynamic DNA reactions on membrane surface.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400748"},"PeriodicalIF":2.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming of Glucose Metabolism for Revisiting Hepatocellular Carcinoma Resistance to Transcatheter Hepatic Arterial Chemoembolization. 重新规划葡萄糖代谢，重新审视肝癌对经导管肝动脉化疗栓塞的耐受性。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-05 DOI: 10.1002/cbic.202400719

Ruijie Zhang, Fan Dai, Songhan Deng, Yun Zeng, Jinyang Wang, Gang Liu

引用次数: 0

Cross-Coupling of Mo- and V-Nitrogenases Permits Protein-Mediated Protection from Oxygen Deactivation Mo-和 V-氮酶的交叉偶联允许蛋白质介导的氧失活保护。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-11-05 DOI: 10.1002/cbic.202400585

Daniel Ratcliff, G. C. Danielle Sedoh, Ross D. Milton

{"title":"Cross-Coupling of Mo- and V-Nitrogenases Permits Protein-Mediated Protection from Oxygen Deactivation","authors":"Daniel Ratcliff, G. C. Danielle Sedoh, Ross D. Milton","doi":"10.1002/cbic.202400585","DOIUrl":"10.1002/cbic.202400585","url":null,"abstract":"Nitrogenases catalyze dinitrogen (N2) fixation to ammonia (NH3). While these enzymes are highly sensitive to deactivation by molecular oxygen (O2) they can be produced by obligate aerobes for diazotrophy, necessitating a mechanism by which nitrogenase can be protected from deactivation. In the bacterium Azotobacter vinelandii, one mode of such protection involves an O2-responsive ferredoxin-type protein (“Shethna protein II”, or “FeSII”) which is thought to bind with Mo-dependent nitrogenase's two component proteins (NifH and NifDK) to form a catalytically stalled yet O2-tolerant tripartite protein complex. This protection mechanism has been reported for Mo-nitrogenase, however, in vitro assays with V-nitrogenase suggest that this mechanism is not universal to the three known nitrogenase isoforms. Here we report that the reductase of the V-nitrogenase (VnfH) can engage in this FeSII-mediated protection mechanism when cross-coupled with Mo-nitrogenase NifDK. Interestingly, the cross-coupling of the Mo-nitrogenase reductase NifH with the V-nitrogenase VnfDGK protein does not yield such protection.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"25 23","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0