ChemBioChem最新文献_第8页

Synthesis of 2-Substituted Adenosine Triphosphate Derivatives and their use in Enzymatic Synthesis and Postsynthetic Labelling of RNA. 2-取代ATP衍生物的合成及其在酶促合成和RNA合成后标记中的应用。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-02 DOI: 10.1002/cbic.202500241

Ugnė Šinkevičiūtė, Tania Sanchez-Quirante, Samanta Rožánková, Lenka Poštová Slavětínská, Veronika Raindlová, Michal Hocek

引用次数: 0

Biosynthesis of Ephedrine is Initiated by Pyridoxal Phosphate-Dependent Formation of Cathinone. 由磷酸吡哆醛依赖性形成卡西酮引发麻黄碱的生物合成。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-05-01 DOI: 10.1002/cbic.202500279

Karina Witte, Anne Behrens, Hannes M Schwelm, Volker Auwärter, Michael Müller

{"title":"Biosynthesis of Ephedrine is Initiated by Pyridoxal Phosphate-Dependent Formation of Cathinone.","authors":"Karina Witte, Anne Behrens, Hannes M Schwelm, Volker Auwärter, Michael Müller","doi":"10.1002/cbic.202500279","DOIUrl":"https://doi.org/10.1002/cbic.202500279","url":null,"abstract":"Ephedra alkaloids possess some of the most basic structures of alkaloids. Despite their importance for human use and their commercial relevance, the biosynthesis of ephedra alkaloids has remained enigmatic. The predominant biosynthetic pathway in the literature proposes a thiamine-dependent caboligation followed by a transaminase, although no candidate enzymes have yet been identified in ephedra alkaloid producers. In this work, an alternative pathway in plants to ephedra alkaloids via (S)-cathinone was investigated that circumvents the formation of 1-phenylpropane-1,2-dione as an intermediate and is in full agreement with previous biosynthetic studies. This alternative pathway involves the pyridoxal phosphate (PLP)-dependent carboligation of benzoyl-CoA and l-alanine in a single step. The PLP-dependent formation of labeled and unlabeled (S)-cathinone was detected in plant lysate of young stem tissue of various Ephedra species that contained Ephedra alkaloids, as well as in young leaf tissue of Catha edulis. The incorporation of labeled nitrogen from l-alanine into (S)-cathinone supports the hypothesis that an α-oxoamine synthase (AOS) catalyzes the formation of (S)-cathinone, bypassing the dione as an intermediate. These results demonstrate the involvement of a PLP-dependent AOS as a pivotal step in the biosynthesis of ephedra alkaloids.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500279"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence Lifetime Multiplexing with Environment-Sensitive Chemigenetic Probes. 荧光寿命复用与环境敏感的化学探针。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-30 DOI: 10.1002/cbic.202500174

Sarah Emmert, Anna Rovira, Pablo Rivera-Fuentes

引用次数: 0

Binding of Hg(I) and Hg(II) Ions to Amyloid-Beta (Aβ) Peptide Variants Affect Their Structure and Aggregation. Hg(I)和Hg（II）离子与淀粉样β (Aβ)肽变体的结合影响其结构和聚集。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-30 DOI: 10.1002/cbic.202500252

Elina Berntsson, Andra Noormägi, Kärt Padari, Jüri Jarvet, Astrid Gräslund, Margus Pooga, Peep Palumaa, Sebastian K T S Wärmländer

{"title":"Binding of Hg(I) and Hg(II) Ions to Amyloid-Beta (Aβ) Peptide Variants Affect Their Structure and Aggregation.","authors":"Elina Berntsson, Andra Noormägi, Kärt Padari, Jüri Jarvet, Astrid Gräslund, Margus Pooga, Peep Palumaa, Sebastian K T S Wärmländer","doi":"10.1002/cbic.202500252","DOIUrl":"https://doi.org/10.1002/cbic.202500252","url":null,"abstract":"Mercury (Hg) exposure is a possible risk factor for Alzheimer´s disease (AD). Some studies reported higher Hg levels in AD patients, but evidence is inconclusive. A mechanism linking Hg exposure to AD neuropathology remains to be found. The hallmark of AD brains is deposits of insoluble amyloid plaques consisting mainly of aggregated amyloid-β (Aβ) peptides. Here, we use transmission electron microscopy (TEM) and biophysical spectroscopy techniques to study in vitro interactions between inorganic Hg and pathologically relevant Aβ(1-40) and Aβ(4-40) variants and the Aβ(1-40)(H6A, H13A, H14A) mutant. For the first time, effect on Aβ aggregation of both Hg(I) and Hg(II) is compared. Hg(II) binds Aβ(1-40) with apparent binding affinity of 28±8 µM, at 20 °C in 20 mM MES buffer, pH 7.3. The N-terminal His6, His13 and His14 residues are involved in binding coordination. Hg(II) binding induces structural alterations (coil-coil interactions) in Aβ monomers positioned in membrane-mimicking SDS micelles. Equimolar amounts of either Hg(I) or Hg(II) inhibit normal Aβ fibrillation by directing aggregation towards formation of large amorphous aggregates. All these structural rearrangements may be relevant for the harmful Aβ aggregation processes involved in AD brain pathology. Inducing protein misfolding and aggregation might be a general toxic mechanism of mercury.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500252"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploiting SpyTag/SpyCatcher Technology to Design New Artificial Catalytic Copper Proteins. 利用SpyTag/SpyCatcher技术设计新型人工催化铜蛋白。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-29 DOI: 10.1002/cbic.202500208

Silvia Gentili, Francesca Miglioli, Valentina Borghesani, Gloria Spagnoli, Denise Bellotti, Davide Cavazzini, Remo Guerrini, Maurizio Remelli, Giovanni Maestri, Simone Ottonello, Angelo Bolchi, Matteo Tegoni

{"title":"Exploiting SpyTag/SpyCatcher Technology to Design New Artificial Catalytic Copper Proteins.","authors":"Silvia Gentili, Francesca Miglioli, Valentina Borghesani, Gloria Spagnoli, Denise Bellotti, Davide Cavazzini, Remo Guerrini, Maurizio Remelli, Giovanni Maestri, Simone Ottonello, Angelo Bolchi, Matteo Tegoni","doi":"10.1002/cbic.202500208","DOIUrl":"10.1002/cbic.202500208","url":null,"abstract":"Designing artificial metal binding sites within a protein is challenging since amino acid residues need to be placed in desired positions in the final construct and the use of non-natural amino acids is difficult. The alternative approach of directing the insertion of artificial metal coordination systems presents the difficulty of grafting such site in a single desired position. Spy protein is composed of a protein component (SpyCatcher) which binds spontaneously an oligopeptide (SpyTag) with formation of an isopeptide bond. A SpyTag peptide equipped with an ATCUN (amino terminal copper and nickel) binding site is designed to bind copper(II) with high femtomolar affinity both in the absence of SpyCatcher and in the reconstituted Spy construct. The Cu2+ ATCUN site in the reconstituted Spy protein presents a catalytic activity in reactive oxygen species production, higher than that of the SpyTag peptide alone. This method offers a novel approach for constructing artificial metalloproteins by incorporating functional metal binding sites into a peptide, which can then be clicked onto its protein counterpart. The small size and modularity of this construct make it versatile for integration into other protein systems, eventually moving the complexity from a protein to a peptide and highlighting its potential for protein design.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500208"},"PeriodicalIF":2.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst? 作用于RNA的小核糖酶的工程：需要什么才能使新功能与现有催化剂协同工作？

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-28 DOI: 10.1002/cbic.202500213

Constanze Ebermann, Sabine Müller

{"title":"Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst?","authors":"Constanze Ebermann, Sabine Müller","doi":"10.1002/cbic.202500213","DOIUrl":"10.1002/cbic.202500213","url":null,"abstract":"The engineering of nucleic acids has been a longstanding objective in research, with the field gaining significant attention following the discovery of ribozymes in the early 1980s. Numerous nucleic acid catalysts have been developed to catalyze a wide range of reactions, and the structures of ribozymes have been modified to allow allosteric regulation by an external cofactor. All these constructs hold considerable promise for applications in biosensors for medical and environmental diagnostics, as well as in molecular tools for regulating cellular processes. In addition to the development of nucleic acid enzymes through in vitro selection, rational design offers a robust strategy for engineering ribozymes with customized properties. The structures and mechanisms of numerous nucleic acid catalysts have been thoroughly elucidated, making structural modulation a viable approach for designing their functional properties. Rational design necessitates the consideration of several parameters, and a range of tools is available to guide sequence design. This review discusses sequence, structural, and functional design, primarily using the example of the hairpin ribozyme, to highlight the challenges and opportunities of rational nucleic acid enzyme engineering.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbic.202500213","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and Achievements of Peptide Synthesis in Aqueous and Micellar Media 在水和胶束介质中合成肽的挑战和成就。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-27 DOI: 10.1002/cbic.202500099

Francesca Bordignon, Alessandro Scarso, Alessandro Angelini

引用次数: 0

Proline Amide Catalyzes Formation of Toxic Crotonaldehyde from Acetaldehyde Under Physiologically Relevant Conditions. 脯氨酸酰胺在生理相关条件下催化乙醛生成有毒的巴豆醛。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-25 DOI: 10.1002/cbic.202500138

Liam A Thomas, Vicki L Emms, Dipti Vashi, Louise Fairall, John W R Schwabe, Richard J Hopkinson

引用次数: 0

Molecular Dynamics Simulations of Monomeric and Tetrameric Amyloid β_1-42 Peptides with d-Aspartic Acid Residues. 含d-天冬氨酸残基的单聚和四聚淀粉样蛋白β1-42肽的分子动力学模拟。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-25 DOI: 10.1002/cbic.202500171

Ayato Mizuno, Tomoki Nakayoshi, Kenju Inaoka, Ayumi Shingaki, Eiji Kurimoto, Koichi Kato, Akifumi Oda

{"title":"Molecular Dynamics Simulations of Monomeric and Tetrameric Amyloid β1-42 Peptides with d-Aspartic Acid Residues.","authors":"Ayato Mizuno, Tomoki Nakayoshi, Kenju Inaoka, Ayumi Shingaki, Eiji Kurimoto, Koichi Kato, Akifumi Oda","doi":"10.1002/cbic.202500171","DOIUrl":"https://doi.org/10.1002/cbic.202500171","url":null,"abstract":"Amyloid β1-42 (Aβ1-42) peptide includes three aspartic acid (Asp) residues. It is known that these Asp residues undergo stereoinversion to d-Asp in ageing tissues, a process that promotes β-sheet structure formation. In this study, the 3D structures of Aβ1-42 monomers and tetramers containing d-Asp residues are analyzed using molecular dynamics (MD) simulations. Seven types of mutants are generated by stereoinverting the three Asp residues, and monomer MD simulations are performed using an implicit solvent model for all seven mutants and the wild type. Following these implicit solvent simulations, tetramer MD simulations using explicit water molecules are conducted for the wild type and three mutants previously reported to form secondary structures in experimental studies. The MD simulations of Aβ1-42 monomers with implicit solvent successfully reproduced the trend of increased β-structure formation caused by D-Asp7 and d-Asp23. However, the effects of d-Asp1 are only captured in tetramer simulations using explicit water. These findings suggest that explicit water is necessary to accurately model peptide-peptide interactions and that multimer simulations are essential for investigating structural features, such as β-sheet formations and aggregation in proteins containing d-amino acids.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500171"},"PeriodicalIF":2.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Covalent Peptide-Graphene Conjugates for Enhanced Cell Spreading, Osteogenic Differentiation, and Angiogenesis in Bone Defects. 共价肽-石墨烯偶联物促进骨缺损细胞扩散、成骨分化和血管生成。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-04-25 DOI: 10.1002/cbic.202500210

Michelle E Wolf, Yaxuan Liu, Jason D Orlando, Jingzhi Zhou, Stefanie A Sydlik

{"title":"Covalent Peptide-Graphene Conjugates for Enhanced Cell Spreading, Osteogenic Differentiation, and Angiogenesis in Bone Defects.","authors":"Michelle E Wolf, Yaxuan Liu, Jason D Orlando, Jingzhi Zhou, Stefanie A Sydlik","doi":"10.1002/cbic.202500210","DOIUrl":"10.1002/cbic.202500210","url":null,"abstract":"Traumatic bone injury is one of the most common injuries that require surgical intervention, and current treatments suffer severe drawbacks. Modern research in bone regeneration focuses on implants that will support and enhance native tissue regeneration. One scaffold material that shows promise is graphene oxide (GO), a 2D nanomaterial made from oxidation of graphite. GO is biocompatible, strong, osteoinductive, is safely and slowly resorbed by the body, has a cheap, facile, and scalable synthesis, and is highly tailorable and functionalizable. The bioactivity of GO can be enhanced via functionalization with biomolecules such as peptides, proteins, and small molecules. Here, short peptides RGD, DGEA, and KKGHK are covalently bound to GO through a Claisen modification (CG) to create new functional graphenic materials that are cell-adhesive, osteogenic, and angiogenic, respectively. These peptide-Claisen graphenes (peptide-CGs) are found to be cytocompatible, to encourage cell spreading on the graphenic surface, to promote osteogenesis in stem cells, and to induce angiogenesis in vascular endothelial cells. They show promise as next-generation bone regeneration scaffolds by overcoming challenges frequently faced by bone regeneration scaffolds, namely retaining implanted and recruited cells, promoting their survival, proliferation, and differentiation, and ensuring a sufficient oxygen and nutrient supply to new tissue.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e2500210"},"PeriodicalIF":2.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0