Nadia Guajardo, Paula Grez, Ricardo S. Schrebler, Rodrigo A. Schrebler
{"title":"Applications of Fluidized Bed Reactors in Biocatalysis","authors":"Nadia Guajardo, Paula Grez, Ricardo S. Schrebler, Rodrigo A. Schrebler","doi":"10.1002/cbic.202500143","DOIUrl":null,"url":null,"abstract":"<p>The main objective of this article is to review previous contributions on the applications of fluidized bed reactors (FBR) in biocatalysis. FBR combines the properties of a stirred tank reactor and a continuous tubular reactor, making it an efficient system for carrying out enzymatic reactions with immobilized enzymes. This equipment's advantages include its high transfer capacity and versatility, as it can be used with liquid and gaseous phases. According to the literature, these devices have been primarily used to degrade contaminants, synthesize cosmetic ingredients, produce food and pharmaceutical compounds, and synthesize biolubricants and biodiesel. The enzymes most used in fluidized bed mode are laccases, lipases, and proteases immobilized on methacrylate resins, mesoporous silicas, alginate, and chitosan beads. Enzyme immobilization is essential, as it can promote the suspension of biocatalyst particles, thereby increasing yields and productivity. One of the leading prospects for these systems is to stabilize the fluidized bed using a magnetic field and the concept of “microfluidization,” which enables the stabilization of smaller biocatalyst particles with smaller equipment, thereby increasing efficiency and intensifying the biocatalytic process. In the future, the versatility of FBR will constitute an attractive alternative for developing biocatalytic systems.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"26 18","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ChemBioChem","FirstCategoryId":"99","ListUrlMain":"https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202500143","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The main objective of this article is to review previous contributions on the applications of fluidized bed reactors (FBR) in biocatalysis. FBR combines the properties of a stirred tank reactor and a continuous tubular reactor, making it an efficient system for carrying out enzymatic reactions with immobilized enzymes. This equipment's advantages include its high transfer capacity and versatility, as it can be used with liquid and gaseous phases. According to the literature, these devices have been primarily used to degrade contaminants, synthesize cosmetic ingredients, produce food and pharmaceutical compounds, and synthesize biolubricants and biodiesel. The enzymes most used in fluidized bed mode are laccases, lipases, and proteases immobilized on methacrylate resins, mesoporous silicas, alginate, and chitosan beads. Enzyme immobilization is essential, as it can promote the suspension of biocatalyst particles, thereby increasing yields and productivity. One of the leading prospects for these systems is to stabilize the fluidized bed using a magnetic field and the concept of “microfluidization,” which enables the stabilization of smaller biocatalyst particles with smaller equipment, thereby increasing efficiency and intensifying the biocatalytic process. In the future, the versatility of FBR will constitute an attractive alternative for developing biocatalytic systems.
期刊介绍:
ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).