ChemBioChem最新文献_第7页

Biosynthesis of the C-Glycosylated Depside Arenicolin B.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-28 DOI: 10.1002/cbic.202500003

Colin W Johnson, Lorraine Nuniz, Bruno Perlatti, Gerald F Bills, Yi Tang

引用次数: 0

β-Turn Mimicking Crosslinking Provides Hyperstability and Fast Folding Kinetics for Short Collagen Triple Helices.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-28 DOI: 10.1002/cbic.202400834

Pengfei Jin, Diane N Rafizadeh, Huanyi Zhao, David M Chenoweth

引用次数: 0

Nanopipettes for Chemical Analysis in Life Sciences.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-27 DOI: 10.1002/cbic.202400879

Shiyu Zheng, Mansha Wu, Xiaoyuan Wang, Shuyue Xu, Ruocan Qian

引用次数: 0

Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-27 DOI: 10.1002/cbic.202500072

Christina Möller, Henrik Terholsen, Ole Schmöker, Thi Linh Anne Lê, Jan Wesche, Paula Schmiade, Esther Eppendorfer, Niklas Rimkus, Britta Girbardt, Dominique Böttcher, Gottfried J Palm, Jens Hoppen, Michael Lammers, Andreas Greinacher, Konstanze Aurich, Uwe T Bornscheuer

{"title":"Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O.","authors":"Christina Möller, Henrik Terholsen, Ole Schmöker, Thi Linh Anne Lê, Jan Wesche, Paula Schmiade, Esther Eppendorfer, Niklas Rimkus, Britta Girbardt, Dominique Böttcher, Gottfried J Palm, Jens Hoppen, Michael Lammers, Andreas Greinacher, Konstanze Aurich, Uwe T Bornscheuer","doi":"10.1002/cbic.202500072","DOIUrl":"10.1002/cbic.202500072","url":null,"abstract":"The supply of blood products such as red blood cells poses a challenge due to rising demand and declining donor numbers. Careful matching of blood products of different types is required. Only type O of the blood types A, B, AB and O can be received by any patient without transfusion incompatibilities. Therefore, O-type blood can be considered \"universal blood\" and is especially needed in emergency situations. In this study, we focused on the conversion of the B antigen by enzymatic deglycosylation to generate the H antigen determining O-type blood. For this, we characterized several previously unstudied α-1,3-galactosidases belonging to the GH110 family. Our findings revealed that the α-1,3-galactosidase from Pedobacter panaciterrae (PpaGal) exhibits superior efficiency compared to previously described galactosidases. We further increased the activity of PpaGal by 2.5-fold using site-directed mutagenesis. Moreover, we solved two crystal structures of PpaGal, one in the apo-state and another in complex with d-galactose. The combination of our mutagenesis study with the solved crystal structures provides valuable information to guide further optimization of PpaGal or other B antigen converting enzymes paving the way for the easier production of universal blood from B-type blood.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500072"},"PeriodicalIF":2.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coelenterazine-dependent Bioluminescence in the Cyclopoid Copepod, Triconia conifera. 环口桡足类三疣梭子蟹的生物发光依赖于腔肠素。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-27 DOI: 10.1002/cbic.202401051

Gaku Mizuno, Shinji Shimode, Yuichiro Nishibe, Yuichi Oba

引用次数: 0

Expanding the Diversity of Nitroxide-Based Paramagnetic Probes Conjugated to Non-Canonical Amino Acids for Sdsl-Epr Applications.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-26 DOI: 10.1002/cbic.202500064

Maxime Bizet, Áron Balázsi, Frédéric Biaso, Deborah Byrne, Emilien Etienne, Bruno Guigliarelli, Philippe Urban, Pierre Dorlet, Gilles Truan, Guillaume Gerbaud, Tamás Kálai, Marlène Martinho

{"title":"Expanding the Diversity of Nitroxide-Based Paramagnetic Probes Conjugated to Non-Canonical Amino Acids for Sdsl-Epr Applications.","authors":"Maxime Bizet, Áron Balázsi, Frédéric Biaso, Deborah Byrne, Emilien Etienne, Bruno Guigliarelli, Philippe Urban, Pierre Dorlet, Gilles Truan, Guillaume Gerbaud, Tamás Kálai, Marlène Martinho","doi":"10.1002/cbic.202500064","DOIUrl":"10.1002/cbic.202500064","url":null,"abstract":"Understanding protein structure requires studying its dynamics, which is critical to elucidating its functional role. Biophysical techniques have revolutionized this field over time, providing remarkable insights into structure-function relationships. Among these, Site-Directed Spin Labelling (SDSL) combined with Electron Paramagnetic Resonance (EPR) is a powerful method delivering structural data at the residue level, irrespective of protein size or environment. Traditional nitroxide labels targeting cysteine residues face limitations when these residues are essential for protein structure or function. To address this, alternatives have been proposed as the use of non-canonical amino acids (ncaa) coupled with specific nitroxide labels. This study introduces 14N-HO-5223, a novel nitroxide label specific to the pAzPhe ncaa, along with its 15N-derivative. These labels were grafted at two sites of the model protein, the diflavin cytochrome P450 reductase. For comparative purpose, two already reported labels were also used. Continuous wave (cw) EPR spectroscopy confirmed the HO-5223 label as an effective reporter of protein dynamics. Additionally, Double Electron-Electron Resonance (DEER) measurements provided distance distributions between the semi-quinone FMNH⋅ state of the CPR and all nitroxide labels. These results expand the toolkit of the ncaa-nitroxide pairs, enabling EPR-based structural studies of proteins where cysteine modification is impractical, further advancing our ability to decode protein dynamics and function.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500064"},"PeriodicalIF":2.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Chemical Biology Insights Towards Reversible Stapled Peptides.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-26 DOI: 10.1002/cbic.202500052

Ying Chen, Chuan Dai, Jinyan Han, Yun Xing, Feng Yin, Zigang Li

{"title":"Recent Chemical Biology Insights Towards Reversible Stapled Peptides.","authors":"Ying Chen, Chuan Dai, Jinyan Han, Yun Xing, Feng Yin, Zigang Li","doi":"10.1002/cbic.202500052","DOIUrl":"10.1002/cbic.202500052","url":null,"abstract":"Peptides are increasingly recognized for their advantages over small molecules in the modulation of protein-protein interactions (PPIs), particularly in terms of potency and selectivity. \"Staples\" can be coupled to the amino acid residues of linear peptides to limit their conformation, improving the stability, membrane permeability, and resistance to proteolysis of peptides. However, the addition of staples can sometimes lead to the complete inactivation of the original peptide or result in extensive interactions that complicate biophysical analysis. Reversible stapled peptides provide an excellent solution to these issues. Besides, probes based on reversible stapled peptides are also indispensable tools for thoroughly investigating PPIs. Consequently, the development of diverse reversible stapling techniques for stapled peptides is crucial for broadening the applications of peptide molecules in drug discovery, drug delivery, and as tools in chemical biology research. This review aims to summarize representative chemical design strategies for reversible stapled peptides, focusing on reversible chemical stapling methods involving sulfhydryl, amino, and methylthio groups, as well as reversible modulation of the conformational states of stapled peptides. Additionally, we demonstrate some intriguing biological applications of stapled peptides and, finally, suggest future research directions in the field that will serve as references for related researchers.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202500052"},"PeriodicalIF":2.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of Polyhydroxyalkanoates From Sucrose by Recombinant Pseudomonas putida KT2440.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-26 DOI: 10.1002/cbic.202401000

Hye Min Song, Seo Hyun Lim, Eun Seo Lee, Dojin Kim, Sang Yup Lee, Ki Jun Jeong, Si Jae Park

{"title":"Biosynthesis of Polyhydroxyalkanoates From Sucrose by Recombinant Pseudomonas putida KT2440.","authors":"Hye Min Song, Seo Hyun Lim, Eun Seo Lee, Dojin Kim, Sang Yup Lee, Ki Jun Jeong, Si Jae Park","doi":"10.1002/cbic.202401000","DOIUrl":"10.1002/cbic.202401000","url":null,"abstract":"A sucrose-utilization pathway was developed in Pseudomonas putida using sacC from Mannheimia succiniciproducens, which encodes a β-fructofuranosidase that hydrolyzes sucrose into glucose and fructose. Excretion of β-fructofuranosidase into the culture medium was confirmed via western blot analysis. In nitrogen-limited cultivation, P. putida expressing SacC produced 10.52 wt % medium-chain-length polyhydroxyalkanoate (MCL-PHA), while P. putida expressing SacC along with poly(3-hydroxybutyrate) [P(3HB)] biosynthesis genes produced 9.16 wt % P(3HB) from sucrose. Batch and fed-batch cultures of recombinant P. putida suggested that the glucose and fructose derived from sucrose can be completely utilized for cell growth and P(3HB) production. In fed-batch cultures, sucrose supplied into the fermentor to maintain its concentration around 20 g/L was rapidly hydrolyzed into glucose and fructose supporting the production of 30.2 g/L P(3HB) with 38.1 wt %. The engineered P. putida reported herein can facilitate the production of PHAs from sucrose, an abundant and inexpensive carbon source.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202401000"},"PeriodicalIF":2.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Silicon Rhodamine-fused Glibenclamide to Label and Detect Malaria-infected Red Blood Cells.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-25 DOI: 10.1002/cbic.202400628

Claudia Bastl, Cindy M Close, Ingo Holtz, Blaise Gatin-Fraudet, Mareike Eis, Michelle Werum, Smilla Konrad, Laura Kneller, Kilian Roßmann, Christiane Huhn, Souvik Ghosh, Julia Ast, Dorien A Roosen, Martin Lehmann, Volker Haucke, Luc Reymond, David J Hodson, Philip Tinnefeld, Kai Johnsson, Viktorija Glembockyte, Nicole Kilian, Johannes Broichhagen

{"title":"A Silicon Rhodamine-fused Glibenclamide to Label and Detect Malaria-infected Red Blood Cells.","authors":"Claudia Bastl, Cindy M Close, Ingo Holtz, Blaise Gatin-Fraudet, Mareike Eis, Michelle Werum, Smilla Konrad, Laura Kneller, Kilian Roßmann, Christiane Huhn, Souvik Ghosh, Julia Ast, Dorien A Roosen, Martin Lehmann, Volker Haucke, Luc Reymond, David J Hodson, Philip Tinnefeld, Kai Johnsson, Viktorija Glembockyte, Nicole Kilian, Johannes Broichhagen","doi":"10.1002/cbic.202400628","DOIUrl":"10.1002/cbic.202400628","url":null,"abstract":"The malaria parasite Plasmodium falciparum affects the lives of millions of people worldwide every year. The detection of replicating parasites within human red blood cells is of paramount importance, requiring appropriate diagnostic tools. Herein, we design and apply a silicon rhodamine-fused glibenclamide (SiR-glib). We first test this far-red fluorescent, fluorogenic and endoplasmic reticulum-targeting sulfonylurea in mammalian cells and pancreatic islets, before characterizing its labeling performance in red blood cells infected with the asexual developmental stages of Plasmodium falciparum. We further combine SiR-glib with a portable smartphone-based microscope to easily and rapidly identify parasitized red blood cells, providing proof of principle for diagnostic use in rural endemic areas without major healthcare facilities.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":" ","pages":"e202400628"},"PeriodicalIF":2.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Synthetic Riboswitches for Tunable Gene Regulation in Mammalian Cells.

IF 2.6 4区生物学

ChemBioChem Pub Date : 2025-02-24 DOI: 10.1002/cbic.202401015

Rushikesh M Khadake, Vaani Arora, Payal Gupta, Ambadas B Rode

引用次数: 0