ChemBioChem最新文献_第6页

Improving CsOAC Activity in Saccharomyces cerevisiae for directed Production of Olivetolic Acid through Rational Design. 通过合理设计提高酿酒酵母中的 CsOAC 活性，以定向生产橄榄醇酸。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-17 DOI: 10.1002/cbic.202400651

Oliver Kayser, Saskia Spitzer, Marco Aras

引用次数: 0

Aptamer-based Immune Checkpoint Inhibition for Cancer Immunotherapy. 基于色素的免疫检查点抑制用于癌症免疫疗法。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-17 DOI: 10.1002/cbic.202400599

Yihao Huang, Guihong Lin, Sinong Liu, Mingying Chen, Chaoyong Yong Yang, Yanling Song

引用次数: 0

Design and evaluation of peptide inhibitors targeting the dimerization of SARS-CoV-2 main protease. 设计和评估针对 SARS-CoV-2 主要蛋白酶二聚化的多肽抑制剂。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-17 DOI: 10.1002/cbic.202400688

Lu Liu, Yi Yang, Zhiyi Zhao, Xiaoying Li, Yian Chen, Shao-Lin Zhang, Aimin Yang

{"title":"Design and evaluation of peptide inhibitors targeting the dimerization of SARS-CoV-2 main protease.","authors":"Lu Liu, Yi Yang, Zhiyi Zhao, Xiaoying Li, Yian Chen, Shao-Lin Zhang, Aimin Yang","doi":"10.1002/cbic.202400688","DOIUrl":"https://doi.org/10.1002/cbic.202400688","url":null,"abstract":"The severe acute respiratory syndrome virus 2 (SARS-CoV-2) seriously impacted public health. The evolutionarily conserved viral chymotrypsin-like main protease (Mpro) is an important target for anti-SARS-CoV-2 drug development. Previous studies have shown that the eight N-terminal amino acids (N8) of SARS-CoV Mpro are essential for its dimerization, and are used to design inhibitors against SARS-CoV Mpro dimerization. Here, we established a simple readout assay using SDS-PAGE and Coomassie blue staining to measure inhibitory activity of N8 peptide derived from SARS-CoV-2 Mpro. To optimize its inhibitory effect, we then modified the side-chain length, charge, and hydrophilicity of the N8 peptide, and introduced a mutated Mpro recognition sequence. As a result, we obtained a series of potent peptide inhibitors against SARS-CoV-2 Mpro, with N8-A24 being the most efficient with an IC50 value of 1.44 mM. We observed that N8-A24 reduced Mpro dimerization with an IC50 value of 0.86 mM. Molecular docking revealed that N8-A24 formed hydrogen bond interactions with critical dimeric interface residues, thus inhibiting its dimerization and activity. In conclusion, our study not only discovers a series of peptide inhibitors targeting the SARS-CoV-2 Mpro dimerization, but also provides a promising strategy for the rational design of new inhibitors against COVID-19.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amide Synthesis from Decarboxylative Coupling of Isocyanates and Carboxylic Acids. 异氰酸酯和羧酸的脱羧偶联酰胺合成。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-17 DOI: 10.1002/cbic.202400770

R Wang, W H Liu

引用次数: 0

The Second Methylation in Psilocybin Biosynthesis Is Enabled by a Hydrogen Bonding Network Extending into the Secondary Sphere Surrounding the Methyltransferase Active Site. 甲基转移酶活性位点周围的氢键网络促成了迷幻药生物合成过程中的第二次甲基化。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-16 DOI: 10.1002/cbic.202400497

Jesse Hudspeth, Kai Rogge, Tobias Wagner, Maximilian Müll, Dirk Hoffmeister, Bernhard Rupp, Sebastiaan Werten

{"title":"The Second Methylation in Psilocybin Biosynthesis Is Enabled by a Hydrogen Bonding Network Extending into the Secondary Sphere Surrounding the Methyltransferase Active Site.","authors":"Jesse Hudspeth, Kai Rogge, Tobias Wagner, Maximilian Müll, Dirk Hoffmeister, Bernhard Rupp, Sebastiaan Werten","doi":"10.1002/cbic.202400497","DOIUrl":"10.1002/cbic.202400497","url":null,"abstract":"The Psilocybe cubensis SAM-dependent methyltransferase, PsiM, catalyzes the last step in the biosynthesis of psilocybin. Likely evolved from monomethylating RNA methyltransferases, PsiM acquired a key amino acid exchange in the secondary sphere of the active site, M247 N, which is responsible for its capacity to dimethylate. Two variants, PsiMN247M and PsiMN247A, were generated to further examine the role of Asn247 for mono- and dimethylation in PsiM. Herein, we present the kinetic profiles of both variants and crystal structures at resolutions between 0.9 and 1.0 Å. Each variant was crystallized as a ternary complex with the non-methylated acceptor substrate, norbaeocystin and S-adenosyl-l-homocysteine, and in a second complex with the cofactor analog, sinefungin, and the monomethylated substrate, baeocystin. Consistent with the inability of the variants to catalyze a second methyl transfer, these structures reveal catalytically non-productive conformations and a high level of disorder of the methylamine group of baeocystin. Additionally, both variants exhibit destabilization in the β5-β7 sheets and a conserved β-turn of the core Rossmann fold, causing 20-fold reduced substrate binding and 2-fold lower catalytic efficiency even with norbaeocystin. Our structural and kinetic analyses of the variants suggest that Asn247 is essential to allow enough space in the active site for multiple methylations while also participating in a network of hydrogen bonds that stabilizes secondary structure elements in the immediate vicinity of the active site for optimal methylation of norbaeocystin.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo and in vitro Reconstitution of Biosynthesis of N-Prenylated Phenazines Revealing Diverse Phenazine-Modifying Enzymes. 在体内和体外重组 N-异戊烯基化吩嗪的生物合成，发现多种吩嗪修饰酶。

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-16 DOI: 10.1002/cbic.202400723

Teruhito Kato, Dan Xia, Taro Ozaki, Tomoyo Nakao, Ping Zhao, Makoto Nishiyama, Taro Shiraishi, Tomohisa Kuzuyama

引用次数: 0

Front Cover: Vinyl 3-(Dimethylamino)propanoate as an Irreversible Acyl Donor Reagent in a Chromatography-free Lipase-Catalyzed Kinetic Resolution of sec-Alcohols (ChemBioChem 20/2024) 封面：3-(二甲基氨基)丙酸乙烯酯作为不可逆酰基给体试剂用于无色谱脂肪酶催化的仲醇动力学解析（ChemBioChem 20/2024）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-16 DOI: 10.1002/cbic.202482001

Beata Zdun, Dr. Paweł Borowiecki

引用次数: 0

Cover Feature: Photoactivated Anticancer Activity of Cobalt(III) Complexes with Naturally Occurring Flavonoids Chrysin and Silibinin (ChemBioChem 20/2024) 封面专题：钴（III）与天然黄酮类化合物 Chrysin 和 Silibinin 的光活化抗癌活性（ChemBioChem 20/2024）

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-16 DOI: 10.1002/cbic.202482002

Jyotirmoy Dutta, Arpan Bera, Aarti Upadhyay, Ashish Kumar Yadav, Samya Banerjee, Tukki Sarkar, Akhtar Hussain

{"title":"Cover Feature: Photoactivated Anticancer Activity of Cobalt(III) Complexes with Naturally Occurring Flavonoids Chrysin and Silibinin (ChemBioChem 20/2024)","authors":"Jyotirmoy Dutta, Arpan Bera, Aarti Upadhyay, Ashish Kumar Yadav, Samya Banerjee, Tukki Sarkar, Akhtar Hussain","doi":"10.1002/cbic.202482002","DOIUrl":"https://doi.org/10.1002/cbic.202482002","url":null,"abstract":"The article 10.1002/cbic.202400484 by Samya Banerjee, Tukki Sarkar, Akhtar Hussain, and co-workers highlights the synthesis, characterization, and evaluation of six new mixed-ligand cobalt(III) complexes for photodynamic therapy (PDT) in cancer treatment. Notably, complexes Co5 and Co6, featuring dipyridophenazine and the naturally occurring flavonoids chrysin and silibinin, exhibit significant toxicity against cervical and lung cancer cells under visible light with low micromolar IC50 values, while showing minimal toxicity in the absence of light. These complexes induce apoptosis via singlet oxygen generation following a type-II PDT pathway, displaying remarkable selectivity and biocompatibility for potential cancer PDT applications.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture>\u0000 </div>\u0000 </figure>\u0000 ","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbic.202482002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142443529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crown-Ether-Based Artificial K⁺ Selective Ionic Filter. 冠醚基人工 K+ 选择性离子过滤器

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-15 DOI: 10.1002/cbic.202400789

Pengyang Xin, Zhihui Jiu, Linlin Shi, Yuqing Shu, Yufei Song, Yonghui Sun

引用次数: 0

Multipartite Fluorogenic Sensors for Monitoring Tyrosine Phosphatase Activity. 用于监测酪氨酸磷酸酶活性的多组分荧光传感器

IF 2.6 4区生物学

ChemBioChem Pub Date : 2024-10-15 DOI: 10.1002/cbic.202400607

Amy Barrios, Daniel T Hansen, Julian Tu, Alison W Bouck, Cheryl L Mathis

{"title":"Multipartite Fluorogenic Sensors for Monitoring Tyrosine Phosphatase Activity.","authors":"Amy Barrios, Daniel T Hansen, Julian Tu, Alison W Bouck, Cheryl L Mathis","doi":"10.1002/cbic.202400607","DOIUrl":"https://doi.org/10.1002/cbic.202400607","url":null,"abstract":"Fluorogenic substrates are essential tools for studying the activity of many enzymes including the protein tyrosine phosphatases (PTPs). Here, we have taken the first step toward the development of genetically encodable sensors for PTP activity using fluorescent and fluorogen-activating proteins. The Fluorescence-Activating and absorption Shifting Tag (FAST) is a small protein that becomes fluorescent upon binding to a small molecule dye. We demonstrate that FAST protein can be used as a sensor for PTP-mediated dephosphorylation of phosphorylated dye molecules. Phosphorylated 4-hydroxybenzylidene rhodanine (pHBR) is not able to bind to the FAST protein and induce fluorescence, but provides a sensitive assay for PTP activity, readily detecting 100 pM concentrations of PTP1B in the presence of FAST with a kcat value of 19 ± 1 s-1 and a KM value of 93 ± 3 µM. In addition, while phosphorylation of the C-terminal peptide of split GFP does not result in appreciable change in fluorescence of the reconstituted protein, phosphorylation of the C-terminal peptide of the split FAST protein abrogates fluorescence. Upon PTP-mediated dephosphorylation of the C-terminal peptide, the ability of the N- and C-terminal components to form a fluorescent complex with the small molecule dye is restored, leading to fluorescence.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0