New Phytologist最新文献

{"title":"SHOT GLASS, an R2R3-MYB transcription factor, promotes gemma cup and gametangiophore development in Marchantia polymorpha.","authors":"Yuuki Sakai,Hideyuki Takami,Shohei Yamaoka,Hirotaka Kato,Hidehiro Fukaki,Takayuki Kohchi,Kimitsune Ishizaki","doi":"10.1111/nph.70337","DOIUrl":"https://doi.org/10.1111/nph.70337","url":null,"abstract":"Many plants reproduce asexually by generating clonal progeny from vegetative tissues, a process known as vegetative reproduction. This reproduction mode contrasts with sexual reproduction, which enhances genetic diversity. The bryophyte Marchantia polymorpha L. adjusts its reproductive strategy in response to seasonal environmental cues, transitioning between vegetative and sexual reproduction. In this study, we identified a gene encoding the R2R3-MYB transcription factor SHOT GLASS (MpSTG) as a critical regulator of gemma cup development. MpSTG was predominantly expressed in the gemma cup, apical notch, and sexual reproductive organs (gametangiophores). MpSTG mutation resulted in the formation of abnormal shot-glass-shaped structures lacking gemmae, which replaced functional gemma cups. Additionally, MpSTG-disrupted plants failed to develop sexual reproductive organs, even under inductive conditions. In Arabidopsis thaliana, the MpSTG ortholog LATERAL ORGAN FUSION1 (AtLOF1) plays a pivotal role in lateral bud formation. We demonstrated that MpSTG can partially compensate for AtLOF1's function in lateral bud formation in A. thaliana. Our findings suggest that MpSTG is a key regulator of vegetative and sexual reproduction in M. polymorpha, and illustrate that evolutionarily conserved developmental mechanisms may function in both the gametophyte generation of bryophytes and the sporophyte generation of angiosperms.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"13 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A critical reassessment of the novel weapons hypothesis and allelopathy as an adaptive strategy that facilitates plant invasion.","authors":"Robert I Colautti,Pedro Madeira Antunes","doi":"10.1111/nph.70415","DOIUrl":"https://doi.org/10.1111/nph.70415","url":null,"abstract":"Plants have evolved an assortment of chemical adaptations that integrate environmental cues with developmental processes to regulate growth and reproduction. A subset of these phytochemicals may be considered allelopathic adaptations if they enhance fitness by suppressing competition for limiting resources. Despite compelling critiques by John Harper almost a half century ago, research on allelopathy nonetheless experienced a revival at the turn of the 21st century that gave rise to a 'novel weapons hypothesis' (NWH) to explain plant invasions. We briefly review the storied history of allelopathy and apply a systematic literature review to scrutinize the NWH from an eco-evolutionary perspective. Although it remains a highly influential metaphor, we find confused definitions and insufficient empirical evidence to support the NWH. Channeling Harper's original critiques, we propose that research on the NWH - and allelopathy more generally - would benefit from field experiments that causally link plant chemistry and competitive interactions to resource availability, fitness components, and population growth. We introduce a set of postulates to help address confusion over definitions and guide empirical tests of the causal processes linking plant chemistry to competitive outcomes, adaptive evolution, and the structure of plant communities.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"13 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR825-5p-regulated TNLs govern Arabidopsis resistance to Tetranychus urticae and Pieris brassicae.","authors":"Irene Rosa-Diaz,Diego Lopez-Marquez,Carmen R Beuzon,Isabel Diaz","doi":"10.1111/nph.70411","DOIUrl":"https://doi.org/10.1111/nph.70411","url":null,"abstract":"MicroRNAs are essential regulators in plant resistance to biotic stresses, but their specific roles in the plant-herbivore context require deeper investigation. Here, we studied how the Arabidopsis miR825-5p differentially modulates certain TNLs (MRT1, MRT2, and MIST1), triggering defensive responses against the sucking acari Tetranychus urticae or the chewing insect Pieris brassicae. We demonstrated that the expression of miR825-5p is downregulated following T. urticae and P. brassicae infestation and identified miRNA TNL targets whose expression is induced by both feeders. miR825-5p downregulates MRT1 and MRT2, acting as a negative modulator of Arabidopsis basal resistance against T. urticae. A similar miR825-5p-mediated regulation of basal resistance, including MRT1 and MRT2 but also MIST1 silencing, is involved in the Arabidopsis response against P. brassicae. Moreover, miR825-5p triggered the production of MIST1-derived secondary interfering RNAs (phasiRNAs) and amplified silencing of MRT1 and MRT2. Taken together, our findings reveal the role of the miR825-5p/TNL module in controlling Arabidopsis response and adapting plant defenses based on the specific threat.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"38 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation and centromere inactivation of fusion chromosomes in two allotetraploid species from the Saccharum complex.","authors":"Zehuai Yu,Yongji Huang,Xikai Yu,Zuhu Deng,Jin Chai,Jiarui Liu,Zhiyun Gong,Wei Yao,Jisen Zhang,Muqing Zhang,Fan Yu,Jiming Jiang","doi":"10.1111/nph.70369","DOIUrl":"https://doi.org/10.1111/nph.70369","url":null,"abstract":"Chromosome fusion can cause a change in the basic chromosome number of a species, thus imposing a major impact on speciation. However, there are few studies on the structure and evolution of fusion chromosomes in plants. Erianthus rockii and Narenga porphyrocoma are allotetraploid species within the Saccharum complex. Both species possess five distinct fusion chromosomes, each resulting from the fusion of two ancestral chromosomes. We developed a high-resolution oligonucleotide (oligo)-based painting technique and constructed a detailed cytogenetic map for each of the 10 fusion chromosomes, enabling visualization of the breakpoints and fusion events of their ancestral chromosomes. We observed several common features associated with the 10 fusion chromosomes. The breakpoints of these fusion chromosomes were consistently located in the proximal regions near the centromeres of the ancestral chromosomes. Each fusion event was accompanied by the inactivation of one of the two ancestral centromeres, along with the loss of the associated centromeric repeats in the inactivated centromere. We conclude that the 10 fusion chromosomes likely originated through different mechanisms, including nested chromosome fusion, end-to-end fusion, and centromere misdivision followed by rejoining of the resulting fragments.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"133 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA elements and their biotechnological applications in plants","authors":"Filip Lastovka, Hadrien Peyret, George P. Lomonossoff, Betty Y.‐W. Chung","doi":"10.1111/nph.70400","DOIUrl":"https://doi.org/10.1111/nph.70400","url":null,"abstract":"SummaryEngineering of plants for improved traits and efficient heterologous protein production can be achieved by modifying or introducing <jats:italic>cis</jats:italic>‐ or <jats:italic>trans</jats:italic>‐acting RNA elements. The function of these elements depends not only on their nucleotide sequence but also on their highly dynamic higher order structures. In this review, we explore RNA regulatory elements with established or potential application in plant biotechnology. We discuss RNA elements involved in translational control, transcript stability, and protein coproduction, as well as RNA domains that mediate conditional expression, RNA decay, or cap‐independent initiation. While some of these elements can be used in transiently or stably transformed plants, others have proven valuable in plant‐based <jats:italic>in vitro</jats:italic> expression systems. Additionally, we highlight RNA elements important for plastid gene expression. Finally, we examine RNA elements that are yet to be applied in plant biotechnology but have been successfully used in other organisms or require further understanding before they can be effectively utilized.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"23 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incorporating responses of traits to changing climates into species distribution models: a path forward","authors":"Shijia Peng, Aaron M. Ellison, Charles C. Davis","doi":"10.1111/nph.70402","DOIUrl":"https://doi.org/10.1111/nph.70402","url":null,"abstract":"SummaryConventional species distribution models (SDMs) typically consider only abiotic factors, thus overlooking critical biotic dimensions, including traits that play an important role in determining species' distributions in changing environments. Process‐based trait SDMs explicitly incorporate traits and have been applied to SDMs. However, their parameterization can be complex and require data that are unavailable for most species. Recently developed hierarchical trait‐based SDMs use widely available data and facilitate the incorporation of traits into SDMs at broad temporal, spatial, and taxonomic scales. However, despite their promise, existing hierarchical trait‐based SDMs fail to accommodate changing trait spaces under different climate conditions. Here, we provide a new, simplified framework for hierarchical trait‐based SDMs that integrates individuals' trait responses into forecasts of species range shifts in response to ongoing climate changes. We further briefly discuss the issue of non‐independence among species in hierarchical trait‐based SDMs. This work will contribute to an improved understanding of how traits affect species distributions along environmental and temporal gradients and facilitate the application of trait‐based SDMs at large scales under future climate change.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"8 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Warm temperature modifies cell fates to reduce stomata production in Arabidopsis","authors":"Josué Saiz‐Pérez, Alexandra Baekelandt, Jonatan Illescas‐Miranda, Lieven Sterck, Marnik Vuylsteke, Eun‐Ji Kim, Boyu Guo, Bénédicte Desvoyes, Crisanto Gutierrez, Eugenia Russinova, Carmen Fenoll, Montaña Mena","doi":"10.1111/nph.70396","DOIUrl":"https://doi.org/10.1111/nph.70396","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Stomatal abundance decrease in Arabidopsis triggered by warm temperature is attributed to PIF4‐mediated repression of <jats:italic>SPEECHLESS</jats:italic> (<jats:italic>SPCH</jats:italic>) expression. We identified the unknown developmental and transcriptional basis of this adaptive response.</jats:list-item> <jats:list-item>We traced stomatal lineages <jats:italic>in vivo</jats:italic> using cell‐identity marker lines and mutants, quantified epidermal traits, and conducted RNA sequencing under oscillating temperatures.</jats:list-item> <jats:list-item>Prolonged warm temperature or PIF4‐overexpression altered cell fates, inducing diverted stomatal precursors (DPs) that lacked stomatal fate, contributing to stomata reduction. DPs originated from meristemoids that lost <jats:italic>SPCH</jats:italic> expression, lacked <jats:italic>MUTE</jats:italic> expression, and exited the cell cycle. Short warm‐temperature pulses allowed later recovery of <jats:italic>SPCH</jats:italic> expression and did not induce DPs or stomata reduction. Comparison of transcriptomes obtained during warm‐temperature pulses with stomatal lineage cell‐specific profiles identified gene expression changes and contrasted their reversibility. Though at warm temperatures, key stomatal drivers were downregulated, most lineages formed stomata through partly modified transcriptional landscapes that promoted uncommitted cell identities and could include noncanonical pathways.</jats:list-item> <jats:list-item>Expression changes in stomatal regulators and cell‐fate changes explain lineage progression under fluctuating temperatures. Since short‐term temperature oscillations prevail in natural conditions, the requirement of long warm‐temperature exposure to trigger DPs would prevent stomata reduction by occasional temperature rises. Promoting uncommitted lineage stages provides flexibility to stomatal development under environmental changes.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"26 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overlooked aspects of CrRLK1L–RALF signaling","authors":"Sébastjen Schoenaers, Kris Vissenberg","doi":"10.1111/nph.70422","DOIUrl":"https://doi.org/10.1111/nph.70422","url":null,"abstract":"SummaryPlant development relies on the ability of cells to coordinate growth with the cell wall's biomechanical status. Rapid alkalinization factors (RALFs) and their <jats:italic>Catharanthus roseus</jats:italic> RLK1‐like (CrRLK1L) kinase receptors form a dynamic signaling system linking wall sensing to growth regulation. RALF–CrRLK1L signaling triggers subminute responses, ion fluxes, wall stiffening, and receptor clustering, positioning it as a core regulator of real‐time growth modulation. RALFs also act structurally by compacting pectins, introducing a feedback loop between wall composition and signaling. Frequent co‐expression and heteromerization of CrRLK1Ls with multiple RALFs argue for a flexible, combinatorial logic rather than simple one‐ligand‐one‐receptor models. This Tansley Insight outlines a conceptual framework in which flexible, modular receptor–ligand networks could underlie dynamic cell wall monitoring.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"21 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABA insensitive 5 mediates abscisic acid regulation of plant photosynthesis by coordinating carbon and nitrogen assimilation","authors":"Qiaoying Long, Yuxuan Huang, Heping Xie, Haohui He, Wenjing Miao, Wenle Xie, Hongjie Lin, Haijian Huang, Zhisheng Zhang, Xinxiang Peng, Guohui Zhu","doi":"10.1111/nph.70409","DOIUrl":"https://doi.org/10.1111/nph.70409","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Photosynthesis is inextricably linked to plant biomass and productivity. Enhancing the capacity for photosynthetic carbon assimilation stands as a pivotal strategy to boost plant photosynthetic efficiency; however, progress remains limited.</jats:list-item> <jats:list-item>We identified the <jats:italic>rog1</jats:italic> (<jats:italic>repressors of glu1‐1 1</jats:italic>) mutant, which was screened as a genetic suppressor of <jats:italic>glu1‐1</jats:italic>, an aberrant variant of <jats:italic>Arabidopsis</jats:italic> ferredoxin‐dependent glutamate synthase (Fd‐GOGAT) impaired in ammonium assimilation and photosynthesis. The <jats:italic>ROG1</jats:italic> gene encodes the abscisic acid (ABA) biosynthesis enzyme ABA2.</jats:list-item> <jats:list-item>Exogenous ABA exerts inhibitory effects on carbon and nitrogen assimilation, thus diminishing photosynthetic rates, and these inhibitions are contingent upon ABA insensitive 5 (ABI5), a key component of the ABA signaling pathway. Further investigation revealed that ABI5 acts as a transcriptional repressor, directly interacting with the promoters of carbon assimilation genes <jats:italic>RCA</jats:italic>, <jats:italic>RBCS2B</jats:italic>, and <jats:italic>RBCS3B</jats:italic>, as well as the nitrogen assimilation gene <jats:italic>GLU1</jats:italic>, thereby impeding their transcriptional activity. Reducing <jats:italic>ABI5</jats:italic> expression led to increased photosynthetic efficiency, growth, and productivity in both <jats:italic>Arabidopsis</jats:italic> and rice.</jats:list-item> <jats:list-item>This study highlights the critical role of ABI5 in plant photosynthesis by coordinating carbon and nitrogen assimilation, presenting a promising approach to enhance photosynthetic efficiency and agricultural productivity.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"21 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Higher adaxial stomatal density is associated with lower grain yield in spring wheat","authors":"Kajal Samantara, Elena Ivandi, Ingmar Tulva, Pirko Jalakas, Banafsheh Khalegh Doust, Anne Ingver, Merko Kärp, Gintaras Brazauskas, Mara Bleidere, Ilmar Tamm, Hanna Hõrak, Ebe Merilo","doi":"10.1111/nph.70416","DOIUrl":"https://doi.org/10.1111/nph.70416","url":null,"abstract":"","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"12 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144701846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}