New Phytologist最新文献

Contrasting CH 4 flux responses to distinct drought regimes and nitrogen enrichment in a temperate steppe 对比ch4通量对温带草原不同干旱制度和氮富集的响应

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-20 DOI: 10.1111/nph.71677

Lan Du, Jiatao Zhang, Yan Shen, Yonghong Luo, Jinbao Zhang, Ru Tian, Zhuwen Xu

{"title":"Contrasting CH 4 flux responses to distinct drought regimes and nitrogen enrichment in a temperate steppe","authors":"Lan Du, Jiatao Zhang, Yan Shen, Yonghong Luo, Jinbao Zhang, Ru Tian, Zhuwen Xu","doi":"10.1111/nph.71677","DOIUrl":"https://doi.org/10.1111/nph.71677","url":null,"abstract":"Summary Drought and nitrogen (N) deposition alter greenhouse gas fluxes, but how distinct drought scenarios interact with N enrichment to regulate the methane (CH 4 ) sink remains poorly understood. We conducted a 4‐yr field experiment in a temperate steppe to examine CH 4 fluxes under three drought regimes – early‐season intense drought (ID), mid‐season chronic drought (CD), and mid‐season reduced rainfall frequency (RF) – and N addition. CH 4 uptake and its driving mechanisms were assessed through field measurements and structural equation modeling, focusing on the interplay between soil physicochemical properties, microbial abundance, and plant‐mediated emissions. All drought treatments reduced the CH 4 sink with divergent temporal dynamics; reduced RF caused the most persistent reduction, while N addition alleviated the inhibitory effects of CD. These shifts were driven by distinct mechanisms: ID acted via lowering previous‐year belowground net primary productivity and increasing dissolved organic N, CD functioned through increased Artemisia frigida cover and reduced arbuscular mycorrhizal fungal abundance, and RF suppressed uptake via the morphological expansion of A. frigida . We conclude that CH 4 sink stability is highly sensitive to drought regime, and that the morphological expansion of specific plants such as A. frigida may partially offset the soil CH 4 sink. ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"37 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single amino‐acid switch reprograms ROP6 signaling to drive functional evolution in plants 单个氨基酸开关重编程ROP6信号以驱动植物的功能进化

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-20 DOI: 10.1111/nph.71650

Liuqin Zhang, Yahong An, Ting Lan, Jinyu Cui, Xinyue Du, Liman Mu, Lina Liu, Mingxuan Xu, QingQing Li, Liping Cheng, Jianyan Zeng, Lei Hou, Yan Pei, Mi Zhang

{"title":"A single amino‐acid switch reprograms ROP6 signaling to drive functional evolution in plants","authors":"Liuqin Zhang, Yahong An, Ting Lan, Jinyu Cui, Xinyue Du, Liman Mu, Lina Liu, Mingxuan Xu, QingQing Li, Liping Cheng, Jianyan Zeng, Lei Hou, Yan Pei, Mi Zhang","doi":"10.1111/nph.71650","DOIUrl":"https://doi.org/10.1111/nph.71650","url":null,"abstract":"Summary Rho‐related GTPases of plants (ROPs) are evolutionarily conserved molecular switches that typically maintain PIN‐formed proteins at the plasma membrane (PM), yet their functional divergence remains poorly understood. Here, we reveal that a single amino‐acid substitution at residue 194 acts as a core determinant, driving the evolutionary split between Brassicaceae and Malvaceae ROP6 orthologs: glycine enforces PM stabilization, whereas alanine licenses endocytic function. In cotton, the Malvaceae‐predominant alanine mutated GhROP6 displays an auxin‐independent manner. The distinct activity is attributed to the recruitment of three GhRIC effectors that directly bind the actin cytoskeleton and promote F‐actin assembly and bundling. The resulting actin reorganization facilitates GhPIN3a internalization and proteolysis, consequently enhancing cotton fiber elongation. Our findings demonstrate that a single amino‐acid change can reprogram ROP signaling and uncover a molecular switch underlying the functional evolution of ROP6 in regulating plant development. ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"13 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative genomics of dark septate endophytes: shedding light on the enigmatic lifestyle and biology of DSEs 暗隔内生菌的比较基因组学：揭示DSEs神秘的生活方式和生物学

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-20 DOI: 10.1111/nph.71652

Sándor Csíkos, Zsolt Merényi, Dániel G. Knapp, Anna Lipzen, Kurt LaButti, Jasmyn Pangilinan, Robert Riley, Sajeet Haridas, Asaf Salamov, Kerrie Barry, Alan Kuo, Elodie Drula, Igor V. Grigoriev, Bernard Henrissat, Francis Martin, László G. Nagy, Gábor M. Kovács

{"title":"Comparative genomics of dark septate endophytes: shedding light on the enigmatic lifestyle and biology of DSEs","authors":"Sándor Csíkos, Zsolt Merényi, Dániel G. Knapp, Anna Lipzen, Kurt LaButti, Jasmyn Pangilinan, Robert Riley, Sajeet Haridas, Asaf Salamov, Kerrie Barry, Alan Kuo, Elodie Drula, Igor V. Grigoriev, Bernard Henrissat, Francis Martin, László G. Nagy, Gábor M. Kovács","doi":"10.1111/nph.71652","DOIUrl":"https://doi.org/10.1111/nph.71652","url":null,"abstract":"Summary Dark septate endophytes (DSE) are widespread root‐colonising fungi with poorly understood lifestyle and ecological functions. We investigated their genomic traits, sexual reproduction signatures, carbohydrate‐active enzyme (CAZyme) and secondary metabolites profiles, and tested whether genomic features distinguish DSEs and other endophytes from fungi with other lifestyles. We also reconstructed ancestral states to clarify the evolutionary origins of DSEs. We analysed 107 Ascomycota genomes representing diverse lifestyles, including nine newly sequenced DSE genomes. The dataset allowed us to test whether secreted enzyme profiles differ between fungi colonising roots vs shoots and those associated with grass vs nongrass hosts. DSEs evolved through multiple independent trajectories and diverse lifestyles and show remarkable metabolic diversity. They possess extensive degradative capacities and some of the largest biosynthetic gene cluster (BGC) richness among fungi. Despite sharing BGC richness with other endophytes, DSEs form a distinct subgroup with fewer cellulose‐ and more hemicellulose‐targeting enzymes. Genomes also revealed genomic signatures associated with fungi colonising grass vs nongrass hosts and root‐ vs shoot‐associated niches. Our comparative genomics approach highlights how genome‐level data can deepen our understanding of endophytic lifestyle and the biology of the enigmatic DSE fungi. ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"43 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary shifts to gynomonoecy correlate with changes in capitulum architecture in South African Helichrysum (Asteraceae) 南非蜡菊（菊科）头状花序结构的变化与向雌雄同体的进化转变相关

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-20 DOI: 10.1111/nph.71668

Zaynab Shaik, Nicola G. Bergh, Carme Blanco‐Gavaldà, G. Anthony Verboom

{"title":"Evolutionary shifts to gynomonoecy correlate with changes in capitulum architecture in South African Helichrysum (Asteraceae)","authors":"Zaynab Shaik, Nicola G. Bergh, Carme Blanco‐Gavaldà, G. Anthony Verboom","doi":"10.1111/nph.71668","DOIUrl":"https://doi.org/10.1111/nph.71668","url":null,"abstract":"Summary Gynomonoecy has evolved repeatedly in Asteraceae, suggesting selective advantages for species whose capitula combine pistillate and bisexual flowers. Theories invoking conspicuous peripheral flowers and achene heteromorphism do not account for gynomonoecy in the southern African‐centred genus Helichrysum , which lacks both these features. We investigated whether gynomonoecy is associated with changes in capitulum architecture in South African Helichrysum , focusing on flower packing density and achene size. We compared flower packing density and achene size between hermaphroditic and gynomonoecious Helichrysum species using phylogenetic generalised least squares and reconstructed ancestral sexual systems using a well‐sampled, time‐calibrated phylogeny. Gynomonoecious species produced nearly twice as many flowers per unit receptacle area as hermaphroditic species and had subtly but significantly smaller achenes. Ancestral‐state reconstruction strongly supported hermaphroditism as ancestral, with gynomonoecy originating independently 18 times. Our results provide the first phylogenetically informed evidence within a single Asteraceae genus that shifts to gynomonoecy correlate with shifts in capitulum architecture. The association between gynomonoecy, denser flower packing, and reduced achene size is consistent with the hypothesis that accentuated resource gradients within capitula and priority access to resources by first‐maturing peripheral flowers may have favoured the evolution of gynomonoecy. ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"14 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell‐type‐specific PIN‐FORMED 3‐mediated polar auxin transport regulates cambial activity and tension wood formation in Populus tomentosa 细胞类型特异性PIN - FORMED 3介导的极性生长素运输调节毛白杨形成层活性和张力材形成

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-18 DOI: 10.1111/nph.71660

Dongmei Xiang, Lingmin Ran, Yuanjin Li, Liqing Zong, Baoxia Li, Qingqing Chu, Mi Zhang, Xiaokang Fu, Lianghanxiao Sun, Shutang Tan, Markus Geisler, Bangjun Wang, Xiaoyan Gu, Keming Luo, Changzheng Xu

{"title":"Cell‐type‐specific PIN‐FORMED 3‐mediated polar auxin transport regulates cambial activity and tension wood formation in Populus tomentosa ","authors":"Dongmei Xiang, Lingmin Ran, Yuanjin Li, Liqing Zong, Baoxia Li, Qingqing Chu, Mi Zhang, Xiaokang Fu, Lianghanxiao Sun, Shutang Tan, Markus Geisler, Bangjun Wang, Xiaoyan Gu, Keming Luo, Changzheng Xu","doi":"10.1111/nph.71660","DOIUrl":"https://doi.org/10.1111/nph.71660","url":null,"abstract":"Summary Auxin is a central regulator of tree secondary growth and forms maxima in the vascular cambium, yet how these maxima are established remains elusive. Here, we functionally characterized two redundant PIN‐FORMED 3 ( PIN3 ) paralogs from Populus tomentosa to define their roles in secondary growth and gravity‐induced tension wood (TW) formation. We employed fluorescent reporter lines, clustered regularly interspaced short palindromic repeats‐generated knockout mutants, phloem‐specific RNA interference transgenic plants and isotopic auxin transport assays to dissect PIN3 functions during secondary growth of poplar stems. Loss of function of PIN3 paralogs severely compromises cambial proliferation, secondary xylem differentiation and TW development. PIN3 proteins display cell‐type‐specific polar localization with basal polarity in phloem cells and outer lateral polarity in endodermal cells. Phloem‐expressed PIN3 facilitates basipetal auxin transport and is required for cambial auxin response. Upon gravistimulation, endodermal PIN3 repolarizes along the gravity vector, correlating with TW formation. Our findings identify a cell‐type‐specific, PIN3‐mediated auxin transport mechanism that contributes to auxin‐driven secondary growth of tree stems. ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"20 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From topology to biological meaning: why plant family classification matters beyond systematics 从拓扑学到生物学意义：为什么植物科分类比系统学更重要

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-18 DOI: 10.1111/nph.71690

Tim Böhnert, Maximilian Weigend

引用次数: 0

Recent plant photosynthate as a driver of ecological and evolutionary processes 近代植物光合作用作为生态和进化过程的驱动因素

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-18 DOI: 10.1111/nph.71637

David Johnson, Hannah M. Griffiths

引用次数: 0

Arabidopsis cyclin‐dependent kinases and MAP65 phosphorylation link microtubule dynamics with salt stress adaptation 拟南芥细胞周期蛋白依赖激酶和MAP65磷酸化将微管动力学与盐胁迫适应联系起来

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-18 DOI: 10.1111/nph.71659

Xiaoxiao Qu, Junjie Zou, Chunxia Zhang, Junling Yue, Kezhen Yang, Jie Le

{"title":"Arabidopsis cyclin‐dependent kinases and MAP65 phosphorylation link microtubule dynamics with salt stress adaptation","authors":"Xiaoxiao Qu, Junjie Zou, Chunxia Zhang, Junling Yue, Kezhen Yang, Jie Le","doi":"10.1111/nph.71659","DOIUrl":"https://doi.org/10.1111/nph.71659","url":null,"abstract":"Summary Plants possess sophisticated regulatory mechanisms to balance growth and stress responses. Cyclin‐dependent kinases (CDKs) are established regulators of cell division and endoreduplication, yet their roles in abiotic stress responses remain poorly understood. Here, using reverse genetics, biochemical, and cell biological approaches, we show that Arabidopsis ( Arabidopsis thaliana ) CDKB1s contribute to salt stress adaptation through a mechanism involving the phosphorylation of MAP65‐1 and MAP65‐2, two microtubule‐associated proteins essential for cytoskeleton dynamics under salt stress. Loss of CDKB1 function enhances salt tolerance, accompanied by attenuated plasma membrane depolarization and reactive oxygen species accumulation. In vitro phosphorylation assays demonstrate that CDKB1;1 directly phosphorylates MAP65‐1 at Ser‐503. Genetic analyses indicate that phospho‐dead MAP65 variants confer enhanced salt tolerance, whereas phosphomimetic variants compromise tolerance even in the cdkb1;1 1;2 background, supporting a critical role for MAP65 phosphorylation status at these sites in salt stress adaptation. We further found that CDKA;1 can similarly interact with and phosphorylate MAP65‐1, suggesting a potential parallel role for CDKA;1 in this process. Collectively, our findings identify a regulatory module involving CDKs, MAP65, and microtubules that links cell cycle kinases and cytoskeleton dynamics with plant salt stress adaptation. ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"20 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No need to exceed: tomato ripening is controlled by a small amount of ethylene 不需要超过：番茄的成熟是由少量乙烯控制的

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-18 DOI: 10.1111/nph.71678

Sara Zenoni, Alessandra Amato

引用次数: 0

MicroRNA 160 controls the auxin response factor–class III peroxidase module and couples salicylic acid and redox signaling networks in the potato hypersensitive response to potato virus Y MicroRNA 160控制生长素反应因子III类过氧化物酶模块，并偶联水杨酸和氧化还原信号网络在马铃薯对马铃薯Y病毒的过敏反应中

IF 9.4 1区生物学

New Phytologist Pub Date : 2026-10-18 DOI: 10.1111/nph.71646

Maja Križnik, Tjaša Lukan, Maja Zagorščak, Anna Coll Rius, Karmen Pogačar, Nastja Marondini, Katja Stare, Valentina Levak, Jitka Široká, Ondřej Novák, Aleš Kladnik, Kristina Gruden

{"title":"MicroRNA 160 controls the auxin response factor–class III peroxidase module and couples salicylic acid and redox signaling networks in the potato hypersensitive response to potato virus Y","authors":"Maja Križnik, Tjaša Lukan, Maja Zagorščak, Anna Coll Rius, Karmen Pogačar, Nastja Marondini, Katja Stare, Valentina Levak, Jitka Široká, Ondřej Novák, Aleš Kladnik, Kristina Gruden","doi":"10.1111/nph.71646","DOIUrl":"https://doi.org/10.1111/nph.71646","url":null,"abstract":"Summary Hypersensitive response (HR) is an effector‐triggered immune response leading to pathogen restriction and local cell death. Small RNAs (sRNAs), mediating RNA silencing, have a well‐established role in antiviral immunity; however, their role in HR has not been addressed to date. We applied a spatially resolved sRNA‐omics approach to uncover changes at the sRNA level and the dynamics of sRNA–gene regulatory networks in potato ( Solanum tuberosum L.) HR to potato virus Y (PVY). We show that miR160 is repressed in cells adjacent to HR lesions in the resistant cultivar, but not in PVY‐sensitive, salicylic acid (SA)‐depleted plants. Beyond its canonical regulation of StARF10 and StARF17 , miR160 controls a broader regulatory network spanning auxin signaling, cell‐wall remodeling, redox homeostasis, and tuberization. Elevated miR160 levels promote susceptibility‐associated traits, reduce thermotolerance, and suppress genes strongly activated in HR. Mechanistically, we identify a novel miR160– StARF10/StARF17 – StPRX28 module that links miR160 repression to the induction of a Class III apoplastic peroxidase in HR, with SA acting upstream. Together, these findings establish miR160 as an important regulatory node that connects SA–auxin–redox signaling pathways and tunes development, defense, and heat resilience, and highlight miR160 as a promising target for fine‐tuning tolerance to multiple stresses with minimal yield loss. ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"9 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0