New Phytologist最新文献

{"title":"A novel intergenic long noncoding RNA WTRL1 regulates tillering and contributes to ideal architecture in wheat","authors":"Wanlin Zhou, Zhiqiang Wang, Xinjian Zhou, Yunzi Jiang, Lang Yu, Min Liang, Xudong Chen, Yuxin Huang, Haoran Shi, Yu Lin, Yaxi Liu","doi":"10.1111/nph.70583","DOIUrl":"https://doi.org/10.1111/nph.70583","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Long noncoding RNAs (lncRNAs) are crucial posttranscriptional regulators of plant growth and resistance. To uncover lncRNAs modulating wheat tillering, we analyzed previously published lncRNA datasets from tillering‐related near‐isogenic lines (NILs) and identified a differentially expressed long intergenic noncoding RNA (lincRNA), designated <jats:italic>Wheat Tiller Related Long noncoding RNA 1</jats:italic> (<jats:italic>WTRL1</jats:italic>), as a candidate regulator.</jats:list-item> <jats:list-item>The spatiotemporal expression pattern of <jats:italic>WTRL1</jats:italic> was analyzed using RT‐qPCR and RNA fluorescence <jats:italic>in situ</jats:italic> hybridization. Functional validation was performed by overexpressing and silencing <jats:italic>WTRL1</jats:italic> in common wheat. Putative targets were identified via transcriptome analysis of transgenic lines and validated using modified dual luciferase reporter (Dual‐Luc) assays.</jats:list-item> <jats:list-item><jats:italic>WTRL1</jats:italic> was found highly expressed in tiller buds. Overexpression of <jats:italic>WTRL1</jats:italic> significantly reduced tiller number and accelerated flowering time, whereas silencing of <jats:italic>WTRL1</jats:italic> increased tiller number and delayed flowering. Transcriptomics and Dual‐Luc assays identified two target genes, <jats:italic>TraesFLD1D01G290100</jats:italic> and <jats:italic>TraesFLD1A01G316100</jats:italic>, potentially mediating these effects.</jats:list-item> <jats:list-item><jats:italic>WTRL1</jats:italic> is a lincRNA that negatively regulates tiller number and promotes flowering in wheat, likely through posttranscriptional regulation of specific target genes. This discovery provides a valuable genetic tool for molecular breeding strategies aimed at optimizing wheat tiller development and flowering time.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"74 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145235325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bounds on stomatal size can explain scaling with stomatal density in forest plants.","authors":"Congcong Liu,Christopher D Muir,Lawren Sack,Ying Li,Li Xu,Mingxu Li,Jiahui Zhang,Hugo Jan de Boer,Xingguo Han,Guirui Yu,Nianpeng He","doi":"10.1111/nph.70626","DOIUrl":"https://doi.org/10.1111/nph.70626","url":null,"abstract":"A prevailing hypothesis posits that achieving higher maximum rates of leaf carbon gain and water loss is constrained by geometry and/or selection to limit the allocation of epidermal area to stomata (fS). Under this 'stomatal-area minimization hypothesis', higher gs,max is associated with greater numbers of smaller stomata because this trait combination increases gs,max with minimal increase in fS, leading to relative conservation of fS semi-independent of gs,max due to coordination in stomatal size, density, and pore depth. An alternative hypothesis is that the evolution of higher gs,max can be enabled by a greater epidermal area allocated to stomata, leading to positive covariation between fS and gs,max; we call this the 'stomatal-area adaptation hypothesis'. Under this hypothesis, the interspecific scaling between gs,max, stomatal density, and stomatal size is a by-product of selection on a moving optimal gs,max. We integrated biophysical and evolutionary quantitative genetic modeling with phylogenetic comparative analyses of a global data set of stomatal density and size from 2408 vascular forest species. The models present specific assumptions of both hypotheses and deduce predictions that can be evaluated with our empirical analyses of forest plants. There are three main results. First, neither the stomatal-area minimization nor adaptation hypothesis is sufficient to be supported. Second, estimates of interspecific scaling from common regression methods cannot reliably distinguish between hypotheses when stomatal size is bounded. Third, we reconcile both hypotheses with the data by including an additional assumption that stomatal size is bounded by a wide range and under selection; we refer to this synthetic hypothesis as the 'stomatal adaptation + bounded size' hypothesis. This study advances our understanding of scaling between stomatal size and density by mathematically describing specific assumptions of competing hypotheses, demonstrating that existing hypotheses are inconsistent with observations, and reconciling these hypotheses with phylogenetic comparative analyses by postulating a synthetic model of selection on gs,max, fS, and stomatal size.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"22 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145235548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential developmental processes in Physcomitrium patens require distinct levels of total activity provided by functionally redundant PpROP GTPases.","authors":"Aude Le Bail,Benedikt Kost,Janina Nüssel,Tamara Isabeau Lolis,David Koch,Hildegard Voll,Sylwia Schulmeister,Alexander Kaier,Karin Ljung,Maria Ntefidou","doi":"10.1111/nph.70603","DOIUrl":"https://doi.org/10.1111/nph.70603","url":null,"abstract":"RHO (RAS homologous) GTPases regulate important cellular and developmental processes in most eukaryotes. Plant-specific ROP (RHO of plants) GTPase families expanded and functionally diversified during the evolution of vascular plants, but contain few members in nonvascular extant relatives of early land plants. Here, a systematic investigation of essential PpROP functions in the development of the nonvascular moss Physcomitrium patens is presented. This investigation was based on: knocking out individually or all possible combinations of each of the four PpROP genes, which encode nearly identical proteins; complementing knockout lines with wild-type (WT) or mutated PpROPs, or with heterologous homologs; and inducing PpROP overexpression. PpROPs were found to have previously unknown functions in cell proliferation, caulonema differentiation, and gametophore formation. PpROP functions were observed to display variable dependence on guanosine diphosphate (GDP)/guanosine triphosphate (GTP) cycling and to rely on distinct downstream signaling. Different cellular and developmental processes were determined to require distinct levels of total PpROP activity, rather than individual PpROPs. These observations provide important insights into PpROP functions and signaling in P. patens, enhancing our understanding of the evolution of the regulation of developmental processes by ROP/RHO GTPases. The evolutionary origin of the remarkable functional integration and sequence conservation within the PpROP family is discussed.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"158 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary dynamics of regulatory genes orchestrate secondary vascular innovation in Populus.","authors":"Xiufang Dai,Jiaman Liu,Wei Li","doi":"10.1111/nph.70630","DOIUrl":"https://doi.org/10.1111/nph.70630","url":null,"abstract":"","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"28 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TCP3-mediated regulation of cell expansion in Arabidopsis thaliana.","authors":"Tomotsugu Koyama,Tadashi Kunieda,Hiromi Toyonaga,Mika Nobuhara,Nobutaka Mitsuda,Kouichi Soga,Junko Ishida,Motoaki Seki,Koji Takahashi,Toshinori Kinoshita,Ayumu Bessho,Taku Demura,Masaru Ohme-Takagi","doi":"10.1111/nph.70631","DOIUrl":"https://doi.org/10.1111/nph.70631","url":null,"abstract":"Cell expansion is crucial for organ morphogenesis in multicellular organisms. Apoplast acidification triggers plant cell expansion. Plant hormones and transcription factors such as TEOSINTE BRANCHED, CYCLOIDEA, and PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTORs (TCPs) control cell expansion. However, details regarding the regulatory mechanism of cell expansion for organ morphogenesis remain unclear. In this study, we used molecular, biochemical, cellular, genetic, atomic force microscopy, and tensile testing analyses and showed that miR319-targeted TCPs integrated cell expansion with organ morphogenesis in Arabidopsis thaliana. We found that TCPs directly induce the expression of genes encoding cell wall-loosening proteins and SMALL AUXIN UP RNAs (SAURs), activators of plasma membrane-localized H+-ATPases. TCP-mediated activation of plasma membrane-localized H+-ATPases stimulates apoplast acidification, reduces cell wall stiffness, promotes cell expansion, and thus exaggerates elongation of the hypocotyl. Ectopic expression of a SAUR gene in sextuple tcp mutant plants substantially recovered the hypocotyl morphology of the tcp mutant, providing genetic evidence of TCP-mediated SAUR regulation. Collectively, our data show that TCPs regulate apoplast acidification for cell expansion.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"47 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis thaliana Cyclic Nucleotide-Gated Channel 19 is involved in root extracellular ATP and Pep1 signalling.","authors":"Youzheng Ning,Bryony C I C Jacobs,Clementine Langlet,Limin Wang,Zhizhong Song,Adeeba M Dark,Elsa Matthus,Sebastian Eves-van den Akker,Taufiq Rahman,Julia M Davies","doi":"10.1111/nph.70624","DOIUrl":"https://doi.org/10.1111/nph.70624","url":null,"abstract":"","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"8 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fifty shades of fade: linking transmittance loss to cellular death during leaf dehydration.","authors":"Alexandre J H Gonzalez,Marylou Mantova,Christelle Blavignac,Hervé Cochard,William M Hammond,N Michele Holbrook,Adam C Graham,Emily E Perry,José M Torres-Ruiz","doi":"10.1111/nph.70627","DOIUrl":"https://doi.org/10.1111/nph.70627","url":null,"abstract":"Drought-induced tree mortality is associated with hydraulic failure and subsequent cell death. Leaf vitality is a critical indicator of tree water stress; however, current methods for assessing cell death are destructive and unsuitable for large-scale measurements. The optical method, which detects changes in leaf light transmittance, has been traditionally used to monitor vein cavitation. However, emerging studies have reported transmittance changes extending beyond veins, suggesting a potential link with mesophyll cell death. In this study, we investigated whether the loss of light transmittance in leaves reflects underlying cellular mortality. We subjected seedlings from six tree species with contrasting drought resistance to progressive water stress. Changes in leaf light transmittance were continuously monitored using the optical method while cell mortality was quantified using classical methods. Cryo-SEM imaging provided anatomical insights into cell structural degradation and its influence on transmittance. Our results revealed a strong synchronicity between transmittance loss and cell mortality increases across species, supporting the use of light transmittance as a nondestructive proxy for cellular damage. Overall, our results highlight the potential of the optical method for early detection of drought-induced stress and mortality in trees and offer a promising, scalable, and noninvasive tool for monitoring tree health under drought.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"31 9 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rooting for function: community‐level fine‐root traits relate to many ecosystem functions","authors":"Kathryn E. Barry, Justus Hennecke, Alexandra Weigelt, Joana Bergmann, Helge Bruelheide, Grégoire T. Freschet, Colleen M. Iversen, Thomas W. Kuyper, Daniel C. Laughlin, M. Luke McCormack, Catherine Roumet, Fons van der Plas, Jasper van Ruijven, Rachel Wijsmuller, Harald Auge, Nico Eisenhauer, Josephine Haase, Charles A. Nock, Yvonne Oelmann, Wolfgang Wilcke, Liesje Mommer","doi":"10.1111/nph.70606","DOIUrl":"https://doi.org/10.1111/nph.70606","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Humans are driving biodiversity change, which also alters community functional traits. However, how changes in the functional traits of the community alter ecosystem functions—especially belowground—remains an important gap in our understanding of the consequences of biodiversity change.</jats:list-item> <jats:list-item>We test hypotheses for how the root traits of the root economics space (composed of the collaboration and conservation gradients) are associated with proxies for ecosystem functioning across grassland and forest ecosystems in both observational and experimental datasets from 810 plant communities. First, we assessed whether community‐weighted means of the root economics space traits adhered to the same trade‐offs as species‐level root traits. Then, we examined the relationships between community‐weighted mean root traits and aboveground biomass production, root standing biomass, soil fauna biomass, soil microbial biomass, decomposition of standard and plot‐specific material, ammonification, nitrification, phosphatase activity, and drought resistance.</jats:list-item> <jats:list-item>We found evidence for a community collaboration gradient but not for a community conservation gradient. Yet, links between community root traits and ecosystem functions were more common than we expected, especially for aboveground biomass, microbial biomass, and decomposition.</jats:list-item> <jats:list-item>These findings suggest that changes in species composition, which alter root trait means, will in turn affect critical ecosystem functions.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"97 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145215633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of squalene epoxidases and oxidosqualene cyclases clarifies roles in BoswelliaC3‐epimeric triterpenoid pathway","authors":"Aashish Kumar, Kapil Dev, Sumit Ghosh","doi":"10.1111/nph.70596","DOIUrl":"https://doi.org/10.1111/nph.70596","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item><jats:italic>Boswellia</jats:italic> trees produce bioactive boswellic acids (BAs) in response to wounding, which are a rare class of C3‐epimeric triterpenoids. Understanding BA biosynthesis, particularly knowing whether C3‐epimerization occurs at the triterpenoid epoxidation/cyclization steps catalyzed by squalene epoxidase (SQE)/2,3‐oxidosqualene cyclase (OSC) or at the later stage of scaffold modification, remains largely elusive.</jats:list-item> <jats:list-item>We identified four BsSQEs (BsSQE1‐4) and six BsOSCs (BsOSC1‐6) from the <jats:italic>Boswellia serrata</jats:italic> transcriptome, and functionally characterized them <jats:italic>in vitro</jats:italic>, <jats:italic>in planta</jats:italic> assays and using yeast SQE/OSC mutants to know the involvement of BsSQEs/BsOSCs in BA biosynthesis.</jats:list-item> <jats:list-item>Wound‐inducible BsSQE1 and BsSQE3 formed (3<jats:italic>S</jats:italic>)‐2,3‐oxidosqualene, and a wound‐inducible BsOSC3 produced α‐amyrin and β‐amyrin. BsSQEs did not make (3<jats:italic>R</jats:italic>)‐2,3‐oxidosqualene, a potential source of C3 epimers, and none of the BsOSCs formed C3 epimers when assayed using racemic (3<jats:italic>R</jats:italic>,<jats:italic>S</jats:italic>)‐2,3‐oxidosqualene. These results excluded the possibility of C3‐epimerization at the squalene epoxidation/2,3‐oxidosqualene cyclization steps. Remarkably, <jats:italic>BsOSC3</jats:italic> overexpression in <jats:italic>B. serrata</jats:italic> leaves increased the contents of 3‐<jats:italic>epi</jats:italic>‐α‐amyrin, 3‐<jats:italic>epi</jats:italic>‐β‐amyrin and BAs, indicating that α/β‐amyrin produced by BsOSC3 might get epimerized and subsequently fed into the BA biosynthetic pathway.</jats:list-item> <jats:list-item>Overall, the results suggested that 3‐<jats:italic>epi</jats:italic>‐α/β‐amyrin is not the direct product of squalene epoxidation/2,3‐oxidosqualene cyclization reactions; rather, 3‐<jats:italic>epi</jats:italic>‐α/β‐amyrin might form via epimerization of α/β‐amyrin, thus establishing a critical step in the BA biosynthetic pathway.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"13 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145215920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Huong Thi Mai To","authors":"","doi":"10.1111/nph.70506","DOIUrl":"https://doi.org/10.1111/nph.70506","url":null,"abstract":"Huong Thi Mai To, University of Science and Technology of Hanoi (Vietnam).","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"11 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}