New Phytologist最新文献

{"title":"Species diversity mediates negative impacts of plant invasion on native-community flowering synchrony.","authors":"Rui-Feng Zhang,Xue Zhang,Long Dang,Yu-Jian Guo,Yu-Han Xu,Ai-Di-Na Yisilamu,Zhao-Gui Yan,Lei Chen,Yong-Jian Wang","doi":"10.1111/nph.70401","DOIUrl":"https://doi.org/10.1111/nph.70401","url":null,"abstract":"Plant invasion is a major global driver to the structure of natural communities. Species diversity is a fundamental determinant of community regeneration through flowering phenology. However, the impacts of plant invasion on native plant flowering phenology and how species diversity regulates the process remain unclear. We conducted a field experiment to assess the effects of plant invasion (Solidago canadensis) and community species diversity on flowering phenology of native plants from different flowering functional groups at the community and species levels. At the community level, plant invasion shortened flowering duration and reduced flowering synchrony. Furthermore, high diversity reduced the negative impacts of plant invasion on flowering synchrony. Among flowering functional groups, the late- and mid-flowering species responded more negatively to plant invasion than the early-flowering species. Structural equation modeling indicated that plant invasion suppressed plant height and first or last flowering date by reducing light availability, which in turn affected flowering phenology. However, high diversity counteracted the negative effects. Our findings confirm the role of high diversity in resisting plant invasions on flowering phenology and reproduction of native plant communities, and suggest that high diversity with different flowering functional groups should be considered to efficiently restore native communities invaded by alien plants, such as S. canadensis.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"21 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental insights into genome reconstruction driven by horizontal transfer of supernumerary chromosomes in Magnaporthe oryzae.","authors":"Zhenyu Fang,Yuyong Li,Jianqiang Huang,Meilian Chen,Xiali Chen,Xueting Mo,Zhenhui Zhong,Xiuxiu Li,Guodong Lu,Guifang Lin,Zonghua Wang,Huakun Zheng","doi":"10.1111/nph.70438","DOIUrl":"https://doi.org/10.1111/nph.70438","url":null,"abstract":"Many pathogenic fungi display 'two-speed genome', with the fast-evolving genomic compartments enriched with repetitive sequences, particularly the transposons, which have been shown to drive the variation of pathogenicity-associated genes. Supernumerary chromosomes (SCs) are known to facilitate genomic variation in fungal pathogens, but their specific role in such processes remains understudied. In this study, we assessed the transferability of SCs between asexual Magnaporthe oryzae strains during co-culture and co-infection, and investigated their role in genome reconstruction through experimental evolution assays. We found that SCs could be horizontally transferred between M. oryzae strains and revealed frequent structural variations facilitated by SCs, including deletions, duplications, translocations, and SC-core chromosome recombinations during and after horizontal transfer. Remarkably, all observed intra- and inter-chromosome rearrangements were confined to core chromosome ends and SCs, indicating a robust role of SCs in facilitating genetic exchange within fast-evolving genomic compartments. Additionally, SC carrying the avirulence gene AvrPikE modulates M. oryzae virulence against Pikh rice through horizontal transfer, loss of whole SC, and segmental deletions. Our findings establish SCs as critical players in shaping the diversity and dynamics of the pathogenic fungal genomes, highlighting them as a cradle for the variation of pathogenicity-associated genes.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"20 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A DELLA-ANAC106 reciprocal negative feedback circuit modulates gibberellin homeostasis to regulate male fertility in Arabidopsis.","authors":"Niu Liu,Yuchen Qian,Qiqi Lu,Xiaofei Han,La Yang,Jiahao Su,Yan Zhang,Min Ni,Yiming Shao,Wangshu Mou,Shuqun Zhang,Juan Xu","doi":"10.1111/nph.70427","DOIUrl":"https://doi.org/10.1111/nph.70427","url":null,"abstract":"While gibberellin (GA) homeostasis is critical for male fertility, the molecular mechanisms governing GA metabolic balance during pollen development remain poorly characterized. This study uncovers a regulatory network maintaining GA equilibrium to ensure male fertility in Arabidopsis thaliana. Through integrated multiomics approaches (DAP-Seq and RNA-Seq) combined with genetic analysis, we identified ANAC106 (AT3G12910), a previously unrecognized NAC (NAM; ATAF1, 2; and CUC2) transcription factor, as a key regulator to maintain GA biosynthesis and signaling homeostasis. Biochemical approaches using protein interaction assays and transcriptional activity tests elucidated a DELLA-ANAC106 feedback mechanism. Gain-of-function and loss-of-function of ANAC106 caused opposing effects on tapetum degradation, both leading to severe male sterility. ANAC106 directly activates GA biosynthetic genes (GA3ox2 and GA3ox4), elevating bioactive GA levels that promote DELLA degradation. The pollen developmental defects observed in ANAC106-overexpressing plants were effectively rescued by overexpressing the DELLA proteins in anthers or developing pollen. Furthermore, DELLA proteins physically interact with ANAC106 in a dose-dependent manner, inhibiting its transcriptional activation capacity to prevent GA over-accumulation. We reveal a DELLA-ANAC106 regulatory circuit that maintains GA homeostasis through reciprocal negative feedback. This self-correcting mechanism ensures precise spatiotemporal control of GA levels during tapetum-pollen coordination, representing a paradigm in phytohormonal regulation of plant reproduction.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"1 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genetic analyses of Arabidopsis autophagy‐related 8 family reveal redundant regulatory roles during autophagy","authors":"Kuntian Dong, Guiling Deng, Yilin Liu, Kuo‐En Chen, Huan Wei, Xiner Huang, Wanying Huang, Ping Zheng, Takashi Ueda, Richard D. Vierstra, Xiao Huang, Faqiang Li","doi":"10.1111/nph.70418","DOIUrl":"https://doi.org/10.1111/nph.70418","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>The ubiquitin‐like protein ATG8 is a central component of the autophagy process and is required at multiple steps during both bulk and selective autophagy. Currently, our understanding of the roles of ATG8 in plants and the possible functional specialization of its family members is limited by genetic redundancy.</jats:list-item> <jats:list-item>Here, we employed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas)9 targeting technology to systematically inactivate all nine <jats:italic>Arabidopsis thaliana</jats:italic> ATG8 loci. Subsequent analyses of the resulting mutants revealed that, unlike mammalian ATG8 family members, which have distinct roles, <jats:italic>Arabidopsis</jats:italic> isoforms largely overlap in their functions controlling autophagic flux. Notably, combinatorial mutations have similarly impaired autophagy and misregulated proteomes much like other autophagy mutants.</jats:list-item> <jats:list-item>We further examined the functional redundancy of <jats:italic>Arabidopsis</jats:italic> ATG8s in late autophagy stages by investigating their interactions with Rab GTPase (RABG)3/RAB7 proteins. We found that all ATG8 representatives could interact with RABG3 proteins via ATG8‐interacting motif–LC3‐interacting region‐docking site interfaces. Such interactions are crucial for RABG3 binding to the autophagosome membrane and probably for the fusion of autophagosomes with the vacuole. However, they are not necessary for endosomal trafficking.</jats:list-item> <jats:list-item>With this collection of multiple high‐order <jats:italic>atg8</jats:italic> mutants, we provide a venue to selectively study the roles of individual ATG8 isoforms during both canonical and noncanonical autophagy in <jats:italic>Arabidopsis</jats:italic>.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"54 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated identification of honey bee pollen loads for field‐applied palynological studies","authors":"Jonathan Barés, Pascal Poncelet, Christine M. Doucet, Charline Legrand, Anais Cambon, Capucine Carlier, Patrick Chevin, Leo‐Paul Dewaele, Delphine Jullien, Jean‐Baptiste Thibaud, Pierre Charnet, Matthieu Rousset, Pierre‐Olivier Cheptou","doi":"10.1111/nph.70435","DOIUrl":"https://doi.org/10.1111/nph.70435","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>In a changing world, it is crucial to characterise communities and their evolution over time. Because social insect pollinators forage on flowering plants around the colony, the nest potentially contains important information about the pollinated plants such as species identity and plant phenology. In this paper, we introduce new approaches to assess plant composition in a Mediterranean summer plant community from pollen foraged by honeybees.</jats:list-item> <jats:list-item>We leveraged the autofluorescence properties of the pollen load to classify plant species, both using a UV/Vis spectrophotometer in the laboratory and a dedicated prototype ‘pollen analyser’ adapted to field studies.</jats:list-item> <jats:list-item>Our results demonstrate that data collected from fluorescent spectra and pollen analyser measurements of pollen load from 14 plant species are specific enough to distinguish plant species. When combined with machine learning techniques, particularly the Support Vector Machine classifier, these approaches provide powerful methods to automatically identify species from fluorescence measurements of pollen load.</jats:list-item> <jats:list-item>Overall, our study shows that analysing the autofluorescence of honeybee pollen load enables the precise identification of their floral origins, paving the way for a real‐time, spatially distributed observatory of flowering plants to monitor species identity, flowering phenology and long‐term ecological dynamics.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"69 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre‐Miocene evolutionary dynamics of tropical rainforests from a dated phylogeny of the palm family","authors":"Gang Yao, Bine Xue, Wei Wang, Craig Barrett, Tong‐Jian Liu, Marinoli Rivas‐Chamorro, Betty Millán, Xue‐Jun Ge","doi":"10.1111/nph.70440","DOIUrl":"https://doi.org/10.1111/nph.70440","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Tropical rainforests (TRFs) are the most species‐rich terrestrial biome on Earth, which experienced a series of global climate fluctuations since the mid‐Cretaceous. However, the relationship between the evolutionary dynamics of TRFs and global climate changes is poorly understood. As an important element of the TRF biome, the palm family Arecaceae provides an ideal model to study the evolutionary history of TRFs.</jats:list-item> <jats:list-item>We present a new palm phylogeny based on combined next‐generation and Sanger sequence data, covering 181/184 genera and 604 of the 2500 accepted species, and calibrated it with 12 fossils to infer the pre‐Miocene temporal evolutionary dynamics of the family.</jats:list-item> <jats:list-item>Results show that Arecaceae started to diversify at <jats:italic>c</jats:italic>. 108.3 million years ago (Ma) during the mid‐Cretaceous, and the diversification rate of the family generally increased during global warming periods and decreased during cooling periods from the mid‐Cretaceous to the end of the Oligocene. The extinction rate of palms increased lastingly since the Oligocene when global temperature was relatively low.</jats:list-item> <jats:list-item>Our data for palms in combination with those from other taxa suggest that marked species turnover might have occurred in the accumulation of species diversity of TRFs during different geological eras under the context of global climate changes.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"31 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PagMIR166c targets PagECH2 to regulate cambial differentiation into secondary xylem, cell expansion, and lignin deposition via different auxin signaling pathways in poplar stems","authors":"Pan Zhao, Xinyi Zhou, Qiulin Yu, Yuting Su, Ran Liu, Shuya Zheng, Huihong Guo","doi":"10.1111/nph.70425","DOIUrl":"https://doi.org/10.1111/nph.70425","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Secondary xylem, a key trait of trees, is the main source of tree biomass. MicroRNAs (miRNAs) play important regulatory roles in the secondary xylem formation; however, the underlying regulatory mechanisms are far from being completely elucidated.</jats:list-item> <jats:list-item>In this study, we provide the first evidence that <jats:italic>PagMIR166c</jats:italic> is involved in the secondary xylem formation in poplar stems. Overexpression of <jats:italic>PagMIR166c</jats:italic> inhibited cambial differentiation into secondary xylem and cell expansion, but promoted lignin deposition and cell wall thickening in poplar stems. Consistently, CRISPR/Cas9‐mediated <jats:italic>pagmir166c</jats:italic> mutants showed an opposite phenotype.</jats:list-item> <jats:list-item>5′ RNA ligase‐mediated rapid amplification of cDNA ends, degradome sequencing, and dual‐luciferase reporter assay showed that <jats:italic>PagMIR166c</jats:italic> targets <jats:italic>PagECH2</jats:italic>, a key gene for IAA biosynthesis. <jats:italic>PagECH2</jats:italic>‐overexpressing (<jats:italic>PagECH2</jats:italic>‐OE) plants showed a phenotype opposite to that of <jats:italic>PagMIR166c</jats:italic>‐overexpressing (<jats:italic>PagMIR166c</jats:italic>‐OE) plants, but similar to that of <jats:italic>pagmir166c</jats:italic> mutants. It was also found that the expression levels of <jats:italic>auxin response factor</jats:italic> genes (<jats:italic>PagARF2</jats:italic>/<jats:italic>PagARF3</jats:italic>/<jats:italic>PagARF4</jats:italic>/<jats:italic>PagARF5</jats:italic>/<jats:italic>PagARF7</jats:italic>) were significantly down‐ or upregulated in <jats:italic>PagMIR166c</jats:italic>‐OE and <jats:italic>PagECH2</jats:italic>‐OE plants as well as <jats:italic>pagmir166c</jats:italic> mutants compared to wild‐type.</jats:list-item> <jats:list-item>Taken together, we identified a novel module, PagMIR166c‐PagECH2, and elucidated the mechanism by which this module regulates the secondary xylem formation via different auxin signaling pathways in poplar stems.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"1 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal repackaging of rainfall magnifies negative impacts of vapor pressure deficit on semiarid ecosystem productivity","authors":"Fangyue Zhang, Joel A. Biederman, Nathan A. Pierce, Jessica S. Guo, Daniel L. Potts, Russell L. Scott, Yongshuo H. Fu, William K. Smith","doi":"10.1111/nph.70431","DOIUrl":"https://doi.org/10.1111/nph.70431","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Many drylands are experiencing less frequent but larger rainfall events alongside rising temperatures and vapor pressure deficit (VPD). How these shifts influence dryland productivity remains unclear.</jats:list-item> <jats:list-item>Using a 4‐yr field experiment in a semiarid grassland, we examined how infrequent but large rainfalls shape VPD constraints on photosynthesis. Ambient conditions over the course of the study spanned 2 yr of extremely high VPD and 2 yr of relatively low VPD, providing a unique opportunity to test the effects of VPD on ecosystem gross primary productivity (GPP) under controlled soil moisture conditions.</jats:list-item> <jats:list-item>GPP declined 39 ± 4% under high VPD even though irrigation treatments and soil moisture levels were unchanged. Daily GPP sensitivity to VPD was strongest under infrequent but large rainfall events, with a 58% ± 19% increase in sensitivity compared to normal rainfall. Another facet of this four‐year study was that deep‐rooted perennials became increasingly dominant through time under infrequent but large rainfalls independent of VPD. Meanwhile, shallow‐rooted annuals became more dominant in frequent but small rainfall treatments and varied with VPD.</jats:list-item> <jats:list-item>These findings underscore how temporally repackaging rainfall into fewer, larger events exacerbates VPD constraints on photosynthesis by driving shifts in vegetation structure, and how an intensified hydrological cycle may impact dryland productivity.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"283 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caroline Dean","authors":"","doi":"10.1111/nph.70423","DOIUrl":"https://doi.org/10.1111/nph.70423","url":null,"abstract":"","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"8 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dorsiventral and angular resistant spectral indices for estimating leaf biochemical traits: scaling from leaf to plant canopy levels","authors":"Dongjie Ran, Zhongqiu Sun, Shan Lu, Kenji Omasa","doi":"10.1111/nph.70417","DOIUrl":"https://doi.org/10.1111/nph.70417","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Leaf biochemical trait estimation is often limited by the dorsiventral asymmetry in leaf surface and internal structures, which causes marked differences in reflection between the adaxial and abaxial sides. In high spatial resolution remote sensing, this challenge is further complicated by leaf orientations, varying solar geometry, and sensor fields of view, which introduce multiangular reflection information. Therefore, mitigating the asymmetry effects of multiangular reflection in dorsiventral leaves is critical for accurate estimation of biochemical traits at both leaf and canopy levels.</jats:list-item> <jats:list-item>Here, we analyzed the multiangular reflection characteristics between two sides of dorsiventral leaves and proposed dorsiventral and angular resistant spectral indices (DARSIs) to estimate leaf Chl content (LCC) and equivalent water thickness based on a theoretical wavelength selection method.</jats:list-item> <jats:list-item>We found substantial variations in the accuracy of existing indices across viewing angles and leaf sides, while DARSIs achieved high accuracy by mitigating the asymmetry effect of multiangular reflection in dorsiventral leaves. When upscaling to the plant canopy level using close‐range hyperspectral images, DARSIs mapped the spatial distribution of LCC more accurately.</jats:list-item> <jats:list-item>Our findings indicate that DARSIs are practical tools for estimating leaf biochemical traits and can provide valuable insights for monitoring plant physiological status.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"121 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}