New Phytologist最新文献

{"title":"Complete pathway elucidation and heterologous reconstitution of (+)-nootkatone biosynthesis from Alpinia oxyphylla","authors":"Xiaomin Deng,&nbsp;Ziling Ye,&nbsp;Jingyu Duan,&nbsp;Fangfang Chen,&nbsp;Yao Zhi,&nbsp;Man Huang,&nbsp;Minjian Huang,&nbsp;Weijia Cheng,&nbsp;Yujie Dou,&nbsp;Zhaolin Kuang,&nbsp;Yanglei Huang,&nbsp;Guangkai Bian,&nbsp;Zixin Deng,&nbsp;Tiangang Liu,&nbsp;Li Lu","doi":"10.1111/nph.19375","DOIUrl":"10.1111/nph.19375","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>(+)-Nootkatone is a natural sesquiterpene ketone widely used in food, cosmetics, pharmaceuticals, and agriculture. It is also regarded as one of the most valuable terpenes used commercially. However, plants contain trace amounts of (+)-nootkatone, and extraction from plants is insufficient to meet market demand. <i>Alpinia oxyphylla</i> is a well-known medicinal plant in China, and (+)-nootkatone is one of the main components within the fruits.</li>\u0000 \u0000 <li>By transcriptome mining and functional screening using a precursor-providing yeast chassis, the complete (+)-nootkatone biosynthetic pathway in <i>Alpinia oxyphylla</i> was identified.</li>\u0000 \u0000 <li>A (+)-valencene synthase (AoVS) was identified as a novel monocot-derived valencene synthase; three (+)-valencene oxidases AoCYP6 (CYP71BB2), AoCYP9 (CYP71CX8), and AoCYP18 (CYP701A170) were identified by constructing a valencene-providing yeast strain. With further characterisation of a cytochrome P450 reductase (AoCPR1) and three dehydrogenases (AoSDR1/2/3), we successfully reconstructed the (+)-nootkatone biosynthetic pathway in <i>Saccharomyces cerevisiae</i>, representing a basis for its biotechnological production.</li>\u0000 \u0000 <li>Identifying the biosynthetic pathway of (+)-nootkatone in <i>A. oxyphylla</i> unravelled the molecular mechanism underlying its formation <i>in planta</i> and also supported the bioengineering production of (+)-nootkatone. The highly efficient yeast chassis screening method could be used to elucidate the complete biosynthetic pathway of other valuable plant natural products in future.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"241 2","pages":"779-792"},"PeriodicalIF":9.4,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabarcoding read abundances of orchid mycorrhizal fungi are correlated to copy numbers estimated using ddPCR","authors":"Deyi Wang,&nbsp;Krijn B. Trimbos,&nbsp;Sofia I. F. Gomes,&nbsp;Hans Jacquemyn,&nbsp;Vincent S. F. T. Merckx","doi":"10.1111/nph.19385","DOIUrl":"10.1111/nph.19385","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"242 4","pages":"1825-1834"},"PeriodicalIF":9.4,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physcomitrium patens response to elevated CO2 is flexible and determined by an interaction between sugar and nitrogen availability","authors":"Boominathan Mohanasundaram,&nbsp;Somnath Koley,&nbsp;Doug K. Allen,&nbsp;Sona Pandey","doi":"10.1111/nph.19348","DOIUrl":"10.1111/nph.19348","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Mosses hold a unique position in plant evolution and are crucial for protecting natural, long-term carbon storage systems such as permafrost and bogs. Due to small stature, mosses grow close to the soil surface and are exposed to high levels of CO₂, produced by soil respiration. However, the impact of elevated CO₂ (eCO₂) levels on mosses remains underexplored.</li>\u0000 \u0000 \u0000 <li>We determined the growth responses of the moss <i>Physcomitrium patens</i> to eCO₂ in combination with different nitrogen levels and characterized the underlying physiological and metabolic changes.</li>\u0000 \u0000 \u0000 <li>Three distinct growth characteristics, an early transition to caulonema, the development of longer, highly pigmented rhizoids, and increased biomass, define the phenotypic responses of <i>P. patens</i> to eCO₂. Elevated CO₂ impacts growth by enhancing the level of a sugar signaling metabolite, T6P. The quantity and form of nitrogen source influences these metabolic and phenotypic changes. Under eCO₂, <i>P. patens</i> exhibits a diffused growth pattern in the presence of nitrate, but ammonium supplementation results in dense growth with tall gametophores, demonstrating high phenotypic plasticity under different environments.</li>\u0000 \u0000 \u0000 <li>These results provide a framework for comparing the eCO₂ responses of <i>P. patens</i> with other plant groups and provide crucial insights into moss growth that may benefit climate change models.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"241 3","pages":"1222-1235"},"PeriodicalIF":9.4,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat tolerance of a tropical–subtropical rainforest tree species Polyscias elegans: time-dependent dynamic responses of physiological thermostability and biochemistry","authors":"Lingling Zhu,&nbsp;Andrew P. Scafaro,&nbsp;Elizabeth Vierling,&nbsp;Marilyn C. Ball,&nbsp;Bradley C. Posch,&nbsp;Frederike Stock,&nbsp;Owen K. Atkin","doi":"10.1111/nph.19356","DOIUrl":"10.1111/nph.19356","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Heat stress interrupts physiological thermostability and triggers biochemical responses that are essential for plant survival. However, there is limited knowledge on the speed plants adjust to heat in hours and days, and which adjustments are crucial.</li>\u0000 \u0000 <li>Tropical–subtropical rainforest tree species (<i>Polyscias elegans</i>) were heated at 40°C for 5 d, before returning to 25°C for 13 d of recovery. Leaf heat tolerance was quantified using the temperature at which minimal chl <i>a</i> fluorescence sharply rose (<i>T</i>_crit). <i>T</i>_crit, metabolites, heat shock protein (HSP) abundance and membrane lipid fatty acid (FA) composition were quantified.</li>\u0000 \u0000 <li><i>T</i>_crit increased by 4°C (48–52°C) within 2 h of 40°C exposure, along with rapid accumulation of metabolites and HSPs. By contrast, it took &gt; 2 d for FA composition to change. At least 2 d were required for <i>T</i>_crit, HSP90, HSP70 and FAs to return to prestress levels.</li>\u0000 \u0000 <li>The results highlight the multi-faceted response of <i>P</i>. <i>elegans</i> to heat stress, and how this response varies over the scale of hours to days, culminating in an increased level of photosynthetic heat tolerance. These responses are important for survival of plants when confronted with heat waves amidst ongoing global climate change.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"241 2","pages":"715-731"},"PeriodicalIF":9.4,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant litter loss exacerbates drought influences on grasslands","authors":"Wanjie Chen,&nbsp;Lin Jiang,&nbsp;Ruoyu Jia,&nbsp;Bo Tang,&nbsp;Hongzhi Jiang,&nbsp;Yang Wang,&nbsp;Xiaoming Lu,&nbsp;Jishuai Su,&nbsp;Yongfei Bai","doi":"10.1111/nph.19374","DOIUrl":"10.1111/nph.19374","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Plant litter is known to affect soil, community, and ecosystem properties. However, we know little about the capacity of litter to modulate grassland responses to climate change.</li>\u0000 \u0000 <li>Using a 7-yr litter removal experiment in a semiarid grassland, here we examined how litter removal interacts with a 2-yr drought to affect soil environments, plant community composition, and ecosystem function.</li>\u0000 \u0000 <li>Litter loss exacerbates the negative impacts of drought on grasslands. Litter removal increased soil temperature but reduced soil moisture and nitrogen mineralization, which substantially increased the negative impacts of drought on primary productivity and the abundance of perennial rhizomatous graminoids. Moreover, complete litter removal shifted plant community composition from grass-dominated to forb-dominated and reduced species and functional group asynchrony, resulting in lower ecosystem temporal stability.</li>\u0000 \u0000 <li>Our results suggest that ecological processes that lead to reduction in litter, such as burning, grazing, and haying, may render ecosystems more vulnerable and impair the capacity of grasslands to withstand drought events.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"241 1","pages":"142-153"},"PeriodicalIF":9.4,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stochastic organelle genome segregation through Arabidopsis development and reproduction","authors":"Amanda K. Broz,&nbsp;Daniel B. Sloan,&nbsp;Iain G. Johnston","doi":"10.1111/nph.19288","DOIUrl":"10.1111/nph.19288","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"241 2","pages":"896-910"},"PeriodicalIF":9.4,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizobium symbiotic efficiency meets CEP signaling peptides","authors":"Carole Laffont,&nbsp;Florian Frugier","doi":"10.1111/nph.19367","DOIUrl":"10.1111/nph.19367","url":null,"abstract":"<p>C-terminally encoded peptides (CEP) signaling peptides are drivers of systemic pathways regulating nitrogen (N) acquisition in different plants, from Arabidopsis to legumes, depending on mineral N availability (e.g. nitrate) and on the whole plant N demand. Recent studies in the <i>Medicago truncatula</i> model legume revealed how root-produced CEP peptides control the root competence for endosymbiosis with N fixing rhizobia soil bacteria through the activity of the Compact Root Architecture 2 (CRA2) CEP receptor in shoots. Among <i>CEP</i> genes, <i>MtCEP7</i> was shown to be tightly linked to nodulation, and the dynamic temporal regulation of its expression reflects the plant ability to maintain a different symbiotic root competence window depending on the symbiotic efficiency of the rhizobium strain, as well as to reinitiate a new window of root competence for nodulation.</p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"241 1","pages":"24-27"},"PeriodicalIF":9.4,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19367","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetically induced Arabidopsis thaliana autotetraploids provide insights into the analysis of meiotic mutants with altered crossover frequency","authors":"Pablo Parra-Nunez,&nbsp;Nadia Fernández-Jiménez,&nbsp;Miguel Pachon-Penalba,&nbsp;Eugenio Sanchez-Moran,&nbsp;Mónica Pradillo,&nbsp;Juan Luis Santos","doi":"10.1111/nph.19366","DOIUrl":"10.1111/nph.19366","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"241 1","pages":"197-208"},"PeriodicalIF":9.4,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19366","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71428094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genomes of Darwin's primroses reveal chromosome-scale adaptive introgression and differential permeability of species boundaries","authors":"Rebecca L. Stubbs,&nbsp;Spyros Theodoridis,&nbsp;Emiliano Mora-Carrera,&nbsp;Barbara Keller,&nbsp;Giacomo Potente,&nbsp;Narjes Yousefi,&nbsp;Paul Jay,&nbsp;Étienne Léveillé-Bourret,&nbsp;Rimjhim Roy Choudhury,&nbsp;Ferhat Celep,&nbsp;Judita Kochjarová,&nbsp;Elena Conti","doi":"10.1111/nph.19361","DOIUrl":"10.1111/nph.19361","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"241 2","pages":"911-925"},"PeriodicalIF":9.4,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19361","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71428095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finding a good balance: two distinct chromatin factors fine-tune stress response in Arabidopsis seedlings","authors":"Rafal Archacki","doi":"10.1111/nph.19357","DOIUrl":"10.1111/nph.19357","url":null,"abstract":"&lt;p&gt;Organisms maintain their integrity and achieve their goals of survival and reproduction by autonomously processing diverse inputs, such as environmental and intrinsic signals, into integrated responses; examples of this include initiating morphogenesis or changing the rate of growth. Plants, as sessile organisms, must continuously adapt to changing environmental conditions. Notable examples are water scarcity or salinity-induced stress faced by seeds or young seedlings, where the plants respond by delaying germination and by arresting postgermination development. These responses can be reversed upon the return of favorable conditions (Finch-Savage &amp; Bassel, &lt;span&gt;2016&lt;/span&gt;; Brandizzi, &lt;span&gt;2020&lt;/span&gt;). While such plasticity offers advantages in nature, it is often undesirable in agricultural settings, as plants may also restrict their growth in response to moderate or temporary stress signals, which can lead to reductions in yield and harvest delays (Gupta &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2020&lt;/span&gt;; Zhang &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2020&lt;/span&gt;). Hence, unraveling the physiological and molecular basis of reaction to stress in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt; (Arabidopsis) and other plant species has been a major task in plant biology. It is now well established that stress responses are accompanied by changes in the activity of hundreds, or even thousands of genes, highlighting the remarkable ability of plants to adapt to conditions prevailing at a particular moment. These broad transcriptional responses are triggered and orchestrated by multiple signaling pathways comprising the action of hormones, transcription factors (TFs), and chromatin modifications (Takahashi &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2018&lt;/span&gt;; Waadt &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;; Zhang &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;). Yet, despite significant progress, the emerging picture is still far from complete. In an article published in this issue of &lt;i&gt;New Phytologist&lt;/i&gt;, Perrella &lt;i&gt;et al&lt;/i&gt;. (&lt;span&gt;2023&lt;/span&gt;; 166–179) report that two distinct chromatin factors, Histone Deacetylase Complex 1 (HDC1) and linker (H1) histones, are involved in shaping the proper transcriptional response to salt stress in Arabidopsis seedlings. They propose a ‘dual brake’ mechanism where HDC1 and H1 impact two different epigenetic marks to suppress some key stress-induced genes, thereby preventing hypersensitive responses.&lt;/p&gt;&lt;p&gt;In addition to its purely structural functions, chromatin is considered to be a major regulatory system that coordinates various genetic networks, including those involved in stress perception and response (Badeaux &amp; Shi, &lt;span&gt;2013&lt;/span&gt;). External and developmental cues can be transmitted through signaling cascades to chromatin-modifying enzymes, including histone acetyltransferases, methyltransferases, and many others (Bannister &amp; Kouzarides, &lt;span&gt;2011&lt;/span&gt;). The introduced modifications can directly or indirectly modulate chromatin structure, affecting its accessibility to differen","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"241 1","pages":"7-9"},"PeriodicalIF":9.4,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19357","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71428079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}