New Phytologist最新文献

{"title":"Arabidopsis cyclin‐dependent kinases and MAP65 phosphorylation link microtubule dynamics with salt stress adaptation","authors":"Xiaoxiao Qu, Junjie Zou, Chunxia Zhang, Junling Yue, Kezhen Yang, Jie Le","doi":"10.1111/nph.71659","DOIUrl":"https://doi.org/10.1111/nph.71659","url":null,"abstract":"Summary <jats:list list-type=\"bullet\"> <jats:list-item> Plants possess sophisticated regulatory mechanisms to balance growth and stress responses. Cyclin‐dependent kinases (CDKs) are established regulators of cell division and endoreduplication, yet their roles in abiotic stress responses remain poorly understood. </jats:list-item> <jats:list-item> Here, using reverse genetics, biochemical, and cell biological approaches, we show that Arabidopsis ( <jats:italic>Arabidopsis thaliana</jats:italic> ) CDKB1s contribute to salt stress adaptation through a mechanism involving the phosphorylation of MAP65‐1 and MAP65‐2, two microtubule‐associated proteins essential for cytoskeleton dynamics under salt stress. </jats:list-item> <jats:list-item> Loss of CDKB1 function enhances salt tolerance, accompanied by attenuated plasma membrane depolarization and reactive oxygen species accumulation. <jats:italic>In vitro</jats:italic> phosphorylation assays demonstrate that CDKB1;1 directly phosphorylates MAP65‐1 at Ser‐503. Genetic analyses indicate that phospho‐dead MAP65 variants confer enhanced salt tolerance, whereas phosphomimetic variants compromise tolerance even in the <jats:italic>cdkb1;1 1;2</jats:italic> background, supporting a critical role for MAP65 phosphorylation status at these sites in salt stress adaptation. We further found that CDKA;1 can similarly interact with and phosphorylate MAP65‐1, suggesting a potential parallel role for CDKA;1 in this process. </jats:list-item> <jats:list-item> Collectively, our findings identify a regulatory module involving CDKs, MAP65, and microtubules that links cell cycle kinases and cytoskeleton dynamics with plant salt stress adaptation. </jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"20 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"No need to exceed: tomato ripening is controlled by a small amount of ethylene","authors":"Sara Zenoni, Alessandra Amato","doi":"10.1111/nph.71678","DOIUrl":"https://doi.org/10.1111/nph.71678","url":null,"abstract":"","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"11 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA 160 controls the auxin response factor–class III peroxidase module and couples salicylic acid and redox signaling networks in the potato hypersensitive response to potato virus Y","authors":"Maja Križnik, Tjaša Lukan, Maja Zagorščak, Anna Coll Rius, Karmen Pogačar, Nastja Marondini, Katja Stare, Valentina Levak, Jitka Široká, Ondřej Novák, Aleš Kladnik, Kristina Gruden","doi":"10.1111/nph.71646","DOIUrl":"https://doi.org/10.1111/nph.71646","url":null,"abstract":"Summary <jats:list list-type=\"bullet\"> <jats:list-item> Hypersensitive response (HR) is an effector‐triggered immune response leading to pathogen restriction and local cell death. Small RNAs (sRNAs), mediating RNA silencing, have a well‐established role in antiviral immunity; however, their role in HR has not been addressed to date. </jats:list-item> <jats:list-item> We applied a spatially resolved sRNA‐omics approach to uncover changes at the sRNA level and the dynamics of sRNA–gene regulatory networks in potato ( <jats:italic>Solanum tuberosum</jats:italic> L.) HR to potato virus Y (PVY). </jats:list-item> <jats:list-item> We show that miR160 is repressed in cells adjacent to HR lesions in the resistant cultivar, but not in PVY‐sensitive, salicylic acid (SA)‐depleted plants. Beyond its canonical regulation of <jats:italic>StARF10</jats:italic> and <jats:italic>StARF17</jats:italic> , miR160 controls a broader regulatory network spanning auxin signaling, cell‐wall remodeling, redox homeostasis, and tuberization. Elevated miR160 levels promote susceptibility‐associated traits, reduce thermotolerance, and suppress genes strongly activated in HR. </jats:list-item> <jats:list-item> Mechanistically, we identify a novel miR160– <jats:italic>StARF10/StARF17</jats:italic> – <jats:italic>StPRX28</jats:italic> module that links miR160 repression to the induction of a Class III apoplastic peroxidase in HR, with SA acting upstream. Together, these findings establish miR160 as an important regulatory node that connects SA–auxin–redox signaling pathways and tunes development, defense, and heat resilience, and highlight miR160 as a promising target for fine‐tuning tolerance to multiple stresses with minimal yield loss. </jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"9 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering uORFs in wheat improves abiotic stress tolerance without growth penalties","authors":"Xiaomin Si, Yonghui Yan, Shenglong Bai, Guanghui Guo, Panpan Xiao, Mengxiao Hu, Kai Xia, Yongqi Ren, Jintao Jiang, Rong Fan, Xiang Ji, Yue Wang, Hao Li, Tingting Dang, Qinmin Gao, Luyu Liu, Changbin Xiong, Kangpeng Liu, Yun Zhou, Chun‐Peng Song","doi":"10.1111/nph.71644","DOIUrl":"https://doi.org/10.1111/nph.71644","url":null,"abstract":"Summary <jats:list list-type=\"bullet\"> <jats:list-item> Developing climate‐resilient crops is critical for ensuring global food security, yet agricultural productivity is frequently constrained by the trade‐off between stress tolerance and growth. This challenge is particularly acute in allohexaploid wheat, a staple crop susceptible to abiotic stresses, underscoring the demand for precise gene regulation. </jats:list-item> <jats:list-item> Here, we established a simple, efficient, and transgene‐free method for regulating gene expression in allohexaploid wheat by disrupting or creating <jats:italic>de novo</jats:italic> upstream open reading frames (uORFs) via genome editing. </jats:list-item> <jats:list-item> Using ribosome profiling, we identified 1199 actively translated uORFs across the wheat genome and developed a dual‐luciferase screening pipeline to pinpoint potent translational repressors of positive regulators of abiotic stress. CRISPR/Cas9‐mediated disruption of the uORFs in <jats:italic>TaCIPK14</jats:italic> and <jats:italic>TaGGP1</jats:italic> elevated downstream protein accumulation, thereby enhancing drought, cold, and salt tolerance of plants. Furthermore, base editing was employed to generate <jats:italic>de novo</jats:italic> uORFs in <jats:italic>TaMADS26,</jats:italic> effectively downregulating gene expression and improving drought resilience. Notably, both the transgene‐free <jats:italic>uorf</jats:italic> <jats:sub> <jats:italic>TaGGP1‐ACG</jats:italic> </jats:sub> and uORF <jats:sub>TaMADS26</jats:sub> mutants conferred pronounced yield advantages under drought conditions without incurring growth penalties under well‐watered conditions. </jats:list-item> <jats:list-item> This study provides a predictable and precise way for modulating gene expression, enabling the coordinated improvement of stress resilience and agronomic performance in wheat. </jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"27 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond sanctions: where the costs of nursery pollination are borne","authors":"Kenji Suetsugu","doi":"10.1111/nph.71679","DOIUrl":"https://doi.org/10.1111/nph.71679","url":null,"abstract":"","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"10 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought‐induced plant dominance shift amplifies functional niche differentiation in steppe communities","authors":"Wang Ma, Xiaosa Liang, Wentao Luo, Bingchuan Zhang, Jiaxin Hu, Jia Guo, Nazim Hassan, Qiang Yu, Xingguo Han, Zhengwen Wang, Yann Hautier","doi":"10.1111/nph.71674","DOIUrl":"https://doi.org/10.1111/nph.71674","url":null,"abstract":"Summary <jats:list list-type=\"bullet\"> <jats:list-item> Trait‐based studies on community assembly under environmental change often overlook intraspecific trait variation (ITV), despite its considerable role in modulating community dynamics, which may largely mislead the inference of community assembly. </jats:list-item> <jats:list-item> Under simulated extreme droughts in a temperate steppe, we integrated ITV and interspecific trait variation for four key traits, plant height (Height), specific leaf area (SLA), leaf dry matter content (LDMC), and leaf thickness (LT), to examine how drought alters functional niche structure and underlying processes. </jats:list-item> <jats:list-item> Drought shifted communities toward conservative resource‐use strategies, reduced community variation in LT, and interspecific trait overlap in terms of SLA, LDMC, LT, and the multivariate trait space, whereas community‐level ITV remained stable across all traits. These changes were driven primarily by diversity loss and increases in the dominance of fewer species, with negligible effects of intraspecific trait plasticity or species turnover. Communities with fewer but more dominant species exhibited smaller trait overlap. </jats:list-item> <jats:list-item> Our findings advance our understanding of the paradigm of trait convergence under environmental filtering. Drought‐induced diversity loss amplifies functional niche differentiation, underscoring the critical role of species reorganization through dominance shifts, with little evidence for intraspecific trait plasticity in mediating community assembly under climate extremes. </jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"37 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PuWRKY46 drives root system architecture and enhances salt tolerance in poplar","authors":"Haoqin Zhao, Shicheng Zhao, Tiantian Sun, Wenhui Zhuang, Jinhui Gao, Houliang Zhang, Jingru Ren, Qianqian Liu, Jingli Yang","doi":"10.1111/nph.71673","DOIUrl":"https://doi.org/10.1111/nph.71673","url":null,"abstract":"Summary <jats:list list-type=\"bullet\"> <jats:list-item> The developmental plasticity of lateral roots (LRs) is an essential adaptive strategy for optimizing root system architecture under salt stress. However, the molecular mechanisms by which salt stress modulates LR plasticity through cell wall remodeling remain largely elusive, particularly in woody species. This study investigates the abscisic acid (ABA)‐responsive transcription factor PuWRKY46 as a critical regulator of LR developmental plasticity under salt stress in <jats:italic>Populus ussuriensis</jats:italic> . </jats:list-item> <jats:list-item> This study integrated phenotypic characterization of transgenic overexpression and RNA interference (RNAi) poplar lines, molecular biochemical assays, protein–protein interaction analyses, transcriptomics, DNA affinity purification sequencing (DAP‐seq), phosphorylation assays, and cell wall biochemical analyses. </jats:list-item> <jats:list-item> Compared with wild‐type plants, <jats:italic>PuWRKY46</jats:italic> overexpression promotes LR emergence and enhances salt tolerance, whereas <jats:italic>PuWRKY46‐</jats:italic> RNAi lines exhibit pronounced salt sensitivity. Furthermore, the mitogen‐activated protein kinase PuMPK15 interacts with PuWRKY46, and the resulting phosphorylation stabilizes PuWRKY46 to enhance salt tolerance. The PuMPK15–PuWRKY46 module targets cell wall‐loosening gene <jats:italic>EXPANSIN‐A11</jats:italic> ( <jats:italic>PuEXPA11</jats:italic> ) to promote localized apoplastic acidification and relax hemicellulose cross‐linking, thereby driving root cell wall remodeling and facilitating LR development. </jats:list-item> <jats:list-item> The PuMPK15–PuWRKY46 signaling cascade regulates salt‐adaptive LR architectural plasticity in poplar. This study reveals a conditional‐plasticity regulatory mechanism and provides a valuable genetic resource for breeding salt‐tolerant woody plants. </jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"11 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unpacking the origins of among‐ and within‐individual variation in pollen quantity and quality: implications for crop yield and breeding","authors":"Marina Micaela Strelin, Marcelo Adrián Aizen","doi":"10.1111/nph.71655","DOIUrl":"https://doi.org/10.1111/nph.71655","url":null,"abstract":"Summary Enhancing crop yields depends on translating fundamental plant biology concepts into practical agricultural solutions – a critical challenge for global food security. Although breeding has historically prioritized yield and stability, the mechanisms underlying variation in key reproductive traits – particularly those determining male fitness – remain largely overlooked. Pollen quantity (grain number) and quality (the ability of a pollen grain to survive, germinate, and sire vigorous offspring) are central to yield and seed quality, yet their biological determinants are rarely integrated into breeding programs. Here, we present a conceptual framework showing how processes such as microspore development, plant aging, and resource allocation – shaped by genetic and environmental factors – generate predictable variation in pollen traits. Rather than reflecting stochastic noise, this variation emerges from coordinated developmental and physiological mechanisms acting across scales, from anther structure to whole‐plant resource dynamics and aging. We argue that crop science must shift from a fragmented, gene‐centered perspective – which relies on phenomenological statistical models such as quantitative trait loci mapping – toward a systemic approach that integrates genes with development, physiology, and the environment to understand variation in pollen quantity and quality. This shift will enable the implementation of targeted strategies to enhance crop yield through male function.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"10 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the epigenetic regulatory network of cotton single‐cell fiber elongation mediated by H3K4me3","authors":"Mengli Yu, Le Liu, Yuling Guo, Yameng Xu, Yujun Li, Xiaonan Li, Yu Wang, Liqiang Fan, Zuoren Yang","doi":"10.1111/nph.71612","DOIUrl":"https://doi.org/10.1111/nph.71612","url":null,"abstract":"Summary <jats:list list-type=\"bullet\"> <jats:list-item> Histone modifications are central to plant development, yet how they coordinate cellular morphogenesis remains poorly understood. Cotton fibers, as single‐celled trichomes derived from seed epidermal cells, provide an ideal model to address this question. </jats:list-item> <jats:list-item> Multi‐omics approaches were used, including cleavage under targets and tagmentation for genome‐wide H3K4me3 profiling and transcriptome sequencing. These were integrated with molecular and genetic validation experiments such as virus‐induced gene silencing, overexpression, CRISPR/Cas9, yeast one/two‐hybrid, electrophoretic mobility shift assay, co‐immunoprecipitation, and split‐luciferase complementation assays. </jats:list-item> <jats:list-item> We identify GhMYBL as a core transcriptional repressor that negatively regulates fiber elongation. <jats:italic>GhMYBL</jats:italic> is subject to dual control: H3K4me3 governs its transcription, whereas GhHBI1 posttranslationally attenuates its DNA‐binding activity. GhMYBL directly represses the positive regulators <jats:italic>GhXTH22</jats:italic> and <jats:italic>GhALP1</jats:italic> , limiting elongation; GhHBI1 relieves this repression, enabling their normal expression and synergistic promotion of fiber elongation. </jats:list-item> <jats:list-item> Our results demonstrate that differential H3K4me3 modification orchestrates GhMYBL‐mediated transcriptional repression, which is fine‐tuned by GhHBI1 interaction to coordinate fiber cell elongation. This study provides mechanistic insights into the epigenetic control of cell elongation and highlights promising targets for cotton fiber improvement. </jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"35 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorylation and epigenetic remodeling mediate BpEBP1 ‐dependent drought resistance in birch","authors":"Shilin Sun, Pengyu Wang, Xue Yang, Jingxin Wang, Jingwen Wang, Xiaofu Li, Siyuan Niu, Xue Han, Di Wu, Chao Wang, Yucheng Wang","doi":"10.1111/nph.71613","DOIUrl":"https://doi.org/10.1111/nph.71613","url":null,"abstract":"Summary <jats:list list-type=\"bullet\"> <jats:list-item> BpEBP1, an ethylene‐responsive factor VII transcription factor, coordinates stress resilience in birch ( <jats:italic>Betula platyphylla</jats:italic> ), but its molecular mechanisms underlying drought response remain unclear. </jats:list-item> <jats:list-item> Using transgenic birch lines (overexpression and knockout), chromatin immunoprecipitation (ChIP)‐seq, biochemical assays, and protein interaction studies, we dissected the multilayered regulatory network of BpEBP1. </jats:list-item> <jats:list-item> BpEBP1 enhances drought tolerance in birch by directly activating key lignin and anthocyanin biosynthesis genes ( <jats:italic>BpMYC2</jats:italic> , <jats:italic>BpCOMT</jats:italic> , and <jats:italic>BpPOD5</jats:italic> ), thereby promoting lignin deposition and anthocyanin accumulation, which reinforce cell wall rigidity and boost reactive oxygen species scavenging capacity. Beyond the canonical GCC‐box <jats:italic>cis</jats:italic> ‐element, BpEBP1 binds to novel Noyes and ZNF449 motifs and uses these <jats:italic>cis</jats:italic> ‐elements to drive expression of the previously described target genes. Mechanistically, BpEBP1 recruits the histone demethylase BpJMJ25 to remove repressive H3K9me2 marks, thereby increasing chromatin accessibility to facilitate transcriptional activation. Meanwhile, drought‐induced phosphorylation at Thr148 by BpEDR1 specifically increases the DNA‐binding affinity of BpEBP1, without affecting its transactivation activity or interaction with BpJMJ25. </jats:list-item> <jats:list-item> BpEBP1 functions as a central regulatory hub that unites transcriptional activation of anthocyanin and lignin‐related genes, BpJMJ25‐mediated H3K9me2 epigenetic remodeling, and BpEDR1‐dependent Thr148 phosphorylation. These two regulatory layers act independently yet synergistically, collectively activating drought‐adaptive metabolic pathways and conferring robust drought resistance in birch. </jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"65 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}