New Phytologist最新文献

{"title":"An allometry perspective on crops","authors":"Adrianus J. Westgeest,&nbsp;François Vasseur,&nbsp;Brian J. Enquist,&nbsp;Rubén Milla,&nbsp;Alicia Gómez-Fernández,&nbsp;David Pot,&nbsp;Denis Vile,&nbsp;Cyrille Violle","doi":"10.1111/nph.20129","DOIUrl":"10.1111/nph.20129","url":null,"abstract":"<p>Understanding trait–trait coordination is essential for successful plant breeding and crop modeling. Notably, plant size drives variation in morphological, physiological, and performance-related traits, as described by allometric laws in ecology. Yet, as allometric relationships have been limitedly studied in crops, how they influence and possibly limit crop performance remains unknown. Here, we review how an allometry perspective on crops gains insights into the phenotypic evolution during crop domestication, the breeding of varieties adapted to novel conditions, and the prediction of crop yields. As allometry is an active field of research, modeling and manipulating crop allometric relationships can help to develop more resilient and productive agricultural systems to face future challenges.</p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1223-1237"},"PeriodicalIF":8.3,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142236224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic ROS and retrograde signaling pathways","authors":"Keun Pyo Lee,&nbsp;Chanhong Kim","doi":"10.1111/nph.20134","DOIUrl":"10.1111/nph.20134","url":null,"abstract":"<p>Sessile plants harness mitochondria and chloroplasts to sense and adapt to diverse environmental stimuli. These complex processes involve the generation of pivotal signaling molecules, including reactive oxygen species (ROS), phytohormones, volatiles, and diverse metabolites. Furthermore, the specific modulation of chloroplast proteins, through activation or deactivation, significantly enhances the plant's capacity to engage with its dynamic surroundings. While existing reviews have extensively covered the role of plastidial retrograde modules in developmental and light signaling, our focus lies in investigating how chloroplasts leverage photosynthetic ROS to navigate environmental fluctuations and counteract oxidative stress, thereby sustaining primary metabolism. Unraveling the nuanced interplay between photosynthetic ROS and plant stress responses holds promise for uncovering new insights that could reinforce stress resistance and optimize net photosynthesis rates. This exploration aspires to pave the way for innovative strategies to enhance plant resilience and agricultural productivity amidst changing environmental conditions.</p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1183-1198"},"PeriodicalIF":8.3,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142236230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streamlined screening platforms lead to the discovery of pachysiphine synthase from Tabernanthe iboga","authors":"Mohamed O. Kamileen,&nbsp;Yoko Nakamura,&nbsp;Katrin Luck,&nbsp;Sarah Heinicke,&nbsp;Benke Hong,&nbsp;Maite Colinas,&nbsp;Benjamin R. Lichman,&nbsp;Sarah E. O'Connor","doi":"10.1111/nph.20133","DOIUrl":"10.1111/nph.20133","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1437-1449"},"PeriodicalIF":8.3,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142236223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The small RNA biogenesis in rice is regulated by MAP kinase-mediated OsCDKD phosphorylation","authors":"Dhanraj Singh,&nbsp;Neetu Verma,&nbsp;Balakrishnan Rengasamy,&nbsp;Gopal Banerjee,&nbsp;Alok Krishna Sinha","doi":"10.1111/nph.20116","DOIUrl":"10.1111/nph.20116","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>CDKs are the master regulator of cell division and their activity is controlled by the regulatory subunit cyclins and phosphorylation by the CAKs. However, the role of MAP kinases in regulating plant cell cycle or CDKs have not been explored.</li>\u0000 \u0000 \u0000 <li>Here, we report that the MAP kinases OsMPK3, OsMPK4, and OsMPK6 physically interact and phosphorylate OsCDKD and its regulatory subunit OsCYCH in rice. MAP kinases phosphorylate CDKD at Ser-168 and Thr-235 residues in OsCDKD. The MAP kinase-mediated phosphorylation of OsCDKD is required for its activation to control the small RNA biogenesis. The phosphodead version of OsCDKD fails to activate the C-terminal domain of RNA Polymerase II, thereby negatively impacting small RNA transcription.</li>\u0000 \u0000 \u0000 <li>Further, the overexpression lines of wild-type (WT) <i>OsCDKD</i> and phosphomimic <i>OsCDKD</i> show increased root growth, plant height, tiller number, panicle number, and seed number in comparison to WT, phosphodead <i>OsCDKD</i>-OE, and kinase-dead <i>OsCDKD</i>-OE plants.</li>\u0000 \u0000 \u0000 <li>In a nutshell, our study establishes a novel regulation of OsCDKD by MAPK-mediated phosphorylation in rice. The phosphorylation of OsCDKD by MAPKs imparts a positive effect on rice growth and development by regulating miRNAs transcription.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1482-1497"},"PeriodicalIF":8.3,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142236225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen sulfide antagonizes cytokinin to change root system architecture through persulfidation of CKX2 in Arabidopsis","authors":"Xiuyu Wang,&nbsp;Cuixia Liu,&nbsp;Tian Li,&nbsp;Fangyu Zhou,&nbsp;Haotian Sun,&nbsp;Fali Li,&nbsp;Ying Ma,&nbsp;Honglei Jia,&nbsp;Xiaoyue Zhang,&nbsp;Wei Shi,&nbsp;Chunmei Gong,&nbsp;Jisheng Li","doi":"10.1111/nph.20122","DOIUrl":"10.1111/nph.20122","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Hydrogen sulfide (H₂S) is an endogenous gaseous signaling molecule, which has been shown to play an important role in plant growth and development by coupling with various phytohormones. However, the relationship between H₂S and cytokinin (CTK) and the mechanisms by which H₂S and CTK affect root growth remain poorly understood.</li>\u0000 \u0000 \u0000 <li>Endogenous CTK was analyzed by UHPLC-ESI-MS/MS. Persulfidation of cytokinin oxidase/dehydrogenases (CKXs) was analyzed by mass spectrometry (MS). <i>ckx2/CKX2</i>^{<i>wild-type (WT)</i>}, OE <i>CKX2</i> and <i>ckx2/CKX2</i>^{<i>Cys(C)62alanine(A)</i>} transgenic lines were isolated with the <i>ckx2</i> background.</li>\u0000 \u0000 \u0000 <li>H₂S is linked to CTK content by CKX2, which regulates root system architecture (RSA). Persulfidation at cysteine (Cys)62 residue of CKX2 enhances CKX2 activity, resulting in reduced CTK content. We utilized <i>35S-LCD/oasa1</i> transgenic lines to investigate the effect of endogenous H₂S on RSA, indicating that H₂S reduces the gravitropic set-point angle (GSA), shortens root hairs, and increases the number of lateral roots (LRs). The persulfidation of CKX2^Cys62 changes the elongation of cells on the upper and lower flanks of LR elongation zone, confirming that Cys62 of CKX2 is the specificity target of H₂S to regulate RSA <i>in vivo</i>.</li>\u0000 \u0000 \u0000 <li>In conclusion, this study demonstrated that H₂S negatively regulates CTK content and affects RSA by persulfidation of CKX2^Cys62 in <i>Arabidopsis thaliana</i>.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1377-1390"},"PeriodicalIF":8.3,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142299242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current challenges for plant biology research in the Global South","authors":"Gabriela Auge,&nbsp;Rohan Shawn Sunil,&nbsp;Robert A. Ingle,&nbsp;Puthan Valappil Rahul,&nbsp;Marek Mutwil,&nbsp;José M. Estevez","doi":"10.1111/nph.20083","DOIUrl":"10.1111/nph.20083","url":null,"abstract":"<p>In an attempt to address the large inequities faced by the plant biology communities from the Global South (i.e. countries located around the tropics and the Southern Hemisphere) at international conferences, this Viewpoint is the reflexive thinking arising from the concurrent session titled ‘Arabidopsis and its translational research in the Global South’ organized at the International Conference of Arabidopsis Research 2023 (ICAR 2023) in Chiba, Japan in June 2023. Here, we highlight the main obstacles plant biology communities in the Global South face in terms of knowledge production, as measured by the unequal production and citation of publications, investigating and advancing local plant genomics and biodiversity, combating disparities in gender and diversity, and current initiatives to break isolation of scientists.</p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1168-1174"},"PeriodicalIF":8.3,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142234050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global patterns and controlling factors of tree bark C : N : P stoichiometry in forest ecosystems consistent with biogeochemical niche hypothesis","authors":"Haiyang Gong,&nbsp;Jordi Sardans,&nbsp;Heng Huang,&nbsp;Zhengbing Yan,&nbsp;Zhiqiang Wang,&nbsp;Josep Peñuelas","doi":"10.1111/nph.20119","DOIUrl":"10.1111/nph.20119","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Bark serves crucial roles in safeguarding trees physically and chemically, while also contributing to nutrient cycling and carbon sequestration. Despite its importance, the broader biogeographical patterns and the potential factors influencing bark C : N : P stoichiometry in forest ecosystems remain largely unknown.</li>\u0000 \u0000 \u0000 <li>In this study, we compiled a comprehensive dataset comprising carbon (C), nitrogen (N), and phosphorus (P) concentrations in bark with 1240 records from 550 diverse forest sites to systematically analyze the large-scale patterns and the factors controlling bark C : N : P stoichiometry.</li>\u0000 \u0000 \u0000 <li>The geometric means of bark C, N, and P concentrations were found to be 493.17 ± 1.75, 3.91 ± 0.09, and 0.2 ± 0.01 mg g⁻¹, respectively. Correspondingly, the C : N, C : P, and N : P mass ratios were 135.51 ± 8.11, 3313.19 ± 210.16, and 19.16 ± 0.6, respectively. Bark C : N : P stoichiometry exhibited conspicuous latitudinal trends, with the exception of N : P ratios. These patterns were primarily shaped by the significant impacts of climate, soil conditions, and plant functional groups. However, the impact of evolutionary history in shaping bark C : N : P stoichiometry outweigh climate, soil, and plant functional group, aligning with the biogeochemical niche (BN) hypothesis.</li>\u0000 \u0000 \u0000 <li>These finding enhance our understanding of the spatial distribution of bark nutrient stoichiometry and have important implications for modeling of global forest ecosystem nutrient cycles in a changing environment.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1303-1314"},"PeriodicalIF":8.3,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142299241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simple, robust, cost-effective, and low-input ChIP-seq method for profiling histone modifications and Pol II in plants","authors":"Danling Zhu,&nbsp;Yi Wen,&nbsp;Yifang Tan,&nbsp;Xi Chen,&nbsp;Zhe Wu","doi":"10.1111/nph.20125","DOIUrl":"10.1111/nph.20125","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Chromatin immunoprecipitation and sequencing (vs ChIP-seq) is an essential tool for epigenetic and molecular genetic studies. Although being routinely used, ChIP-seq is expensive, requires grams of plant materials, and is challenging for samples that enrich fatty acids such as seeds.</li>\u0000 \u0000 \u0000 <li>Here, we developed an Ultrasensitive Plant ChIP-seq (UP-ChIP) method based on native ChIP-seq combined with Tn5 tagmentation-based library construction strategy. UP-ChIP is generally applicable for profiling both histone modification and Pol II in a wide range of plant samples, such as a single Arabidopsis seedling, a few Arabidopsis seeds, and sorted nuclei.</li>\u0000 \u0000 \u0000 <li>Compared with conventional ChIP-seq, UP-ChIP is much less labor intensive and only consumes 1 μg of antibody and 10 μl of Protein-A/G conjugated beads for each IP and can work effectively with the amount of starting material down to a few milligrams. By performing UP-ChIP in various conditions and genotypes, we showed that UP-ChIP is highly reliable, sensitive, and quantitative for studying histone modifications. Detailed UP-ChIP protocol is provided.</li>\u0000 \u0000 \u0000 <li>We recommend UP-ChIP as an alternative to traditional ChIP-seq for profiling histone modifications and Pol II, offering the advantages of reduced labor intensity, decreased costs, and low-sample input.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1658-1669"},"PeriodicalIF":8.3,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142299238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of randomly mutated AlSRKb genes reveals that most loss-of-function mutations cause defects in plasma membrane localization","authors":"Masaya Yamamoto,&nbsp;Shotaro Ohtake,&nbsp;Akihisa Shinozawa,&nbsp;Matsuyuki Shirota,&nbsp;Yuki Mitsui,&nbsp;Hiroyasu Kitashiba","doi":"10.1111/nph.20111","DOIUrl":"10.1111/nph.20111","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1644-1657"},"PeriodicalIF":8.3,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142237014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An auxin homeostat allows plant cells to establish and control defined transmembrane auxin gradients","authors":"Markus Geisler,&nbsp;Ingo Dreyer","doi":"10.1111/nph.20120","DOIUrl":"10.1111/nph.20120","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Extracellular auxin maxima and minima are important to control plant developmental programs. Auxin gradients are provided by the concerted action of proteins from the three major plasma membrane (PM) auxin transporter classes AUX1/LAX, PIN and ATP-BINDING CASSETTE subfamily B (ABCB) transporters. But neither genetic nor biochemical nor modeling approaches have been able to reliably assign the individual roles and interplay of these transporter types.</li>\u0000 \u0000 \u0000 <li>Based on the thermodynamic properties of the transporters, we show here by mathematical modeling and computational simulations that the concerted action of different auxin transporter types allows the adjustment of specific transmembrane auxin gradients. The dynamic flexibility of the ‘auxin homeostat’ comes at the cost of an energy-consuming ‘auxin cycling’ across the membrane.</li>\u0000 \u0000 \u0000 <li>An unexpected finding was that potential functional ABCB-PIN synchronization appears to allow an optimization of the trade-off between the speed of PM auxin gradient adjustment on the one hand and ATP consumption and disturbance of general anion homeostasis on the other.</li>\u0000 \u0000 \u0000 <li>In conclusion, our analyses provide fundamental insights into the thermodynamic constraints and flexibility of transmembrane auxin transport in plants.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 4","pages":"1422-1436"},"PeriodicalIF":8.3,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142299239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}