New Phytologist最新文献

{"title":"Provenance and environment jointly shape early regeneration in fir and beech: evidence from distributed participatory trials across Europe","authors":"Katalin Csilléry, Justine Charlet de Sauvage, Madleina Caduff, Johannes Alt, Marjorie Bison, Mert Çelik, Nicole Ponta, Daniel Wegmann","doi":"10.1111/nph.71627","DOIUrl":"https://doi.org/10.1111/nph.71627","url":null,"abstract":"Summary <jats:list list-type=\"bullet\"> <jats:list-item> Forest regeneration is shaped by strong demographic filters during germination and early establishment, yet the relative contributions of seed provenance and environment remain poorly understood under natural conditions. </jats:list-item> <jats:list-item> We combined climate‐chamber trials with a continental‐scale, field‐based citizen science seed‐sowing experiment to quantify germination, early phenological development, and 3‐yr postgermination survival of fir ( <jats:italic>Abies</jats:italic> spp.) and beech ( <jats:italic>Fagus</jats:italic> spp.) provenances across Europe. We estimated germination metrics, reconstructed phenological trajectories, and quantified successive demographic filters from seed to established seedling. </jats:list-item> <jats:list-item> Germination was structured by provenance‐by‐environment interactions in both genera, but with contrasting expression. Fir showed broadly parallel environmental responses among provenances and partial concordance between climate‐chamber and field results, whereas beech exhibited strong provenance‐by‐environment responses and weak predictability from controlled conditions. Seed weight was a key axis of variation in beech (lighter seeds germinated more frequently and developed faster), but its effects in fir were confounded with geographic differentiation. </jats:list-item> <jats:list-item> Post‐establishment survival was dominated by site‐level environmental filtering and site‐by‐provenance interactions, with limited consistent provenance effects. This reveals a life‐stage shift from fine‐scale provenance‐by‐environment effects during germination and early development to broader environmental control of survival – an important consideration for regeneration‐based management and assisted migration. </jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"21 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149198999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roots to simplicity: when evolution removes a key organ","authors":"Rebecca Fairburn, Alexander Ware, Anthony Bishopp","doi":"10.1111/nph.71639","DOIUrl":"https://doi.org/10.1111/nph.71639","url":null,"abstract":"Summary The evolution of roots was an instrumental event in the colonisation of land by vascular plants. Roots have undergone extensive radiation and can take many diverse forms including taproots, fibrous roots, and adventitious roots. This includes specialist root types, such as pneumatophores in mangroves, that have allowed species to occupy new ecological niches. In some cases, the relationship between roots and soil has been broken, rendering roots unnecessary and ultimately resulting in loss of this organ in some species. We identify several ecological niches/life habits in which rootless vascular plants have evolved: aquatic environments, canopies, arid environments, and carnivory. Many of these plants are challenging to cultivate in a laboratory environment. The exception to this is duckweeds, which we propose as a powerful experimental model for studying organ loss in plants.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"471 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149172946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling epigenetic and genetic variations in response to cold stress in two lotus ecotypes","authors":"Yue Zhang,&nbsp;Xiangyan He,&nbsp;Tao Shi,&nbsp;Zhen Li,&nbsp;Jinming Chen","doi":"10.1111/nph.71570","DOIUrl":"10.1111/nph.71570","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Genetic variations accumulate over long evolutionary timescales, whereas epigenetic modifications can arise rapidly and be inherited across generations. However, the interplay between genetic and epigenetic variations in shaping ecotype-specific phenotypic plasticity remains elusive.</li>\u0000 \u0000 \u0000 <li>Focusing on two lotus ecotypes that evolved under distinct winter temperature regions and display divergent annual growth cycles under cold stress, we generated DNA methylation landscapes across three sequence contexts (CG, CHG, and CHH, where H = A, T, or C) and identified single-cytosine methylation polymorphisms (SMPs) and single-nucleotide polymorphisms (SNPs). Interestingly, only CG methylation patterns mirror population-level genetic variations.</li>\u0000 \u0000 \u0000 <li>Using epigenetic genome-wide association analysis, we identified differentially methylated CG sites that are either <i>cis</i>- or <i>trans</i>-regulated by SNP. Notably, we constructed a multifactorial regulatory network centered on the <i>NnMKK4</i>-<i>NnCYCD5</i> module, linking cold response with cell cycle regulation. Temperature stress experiments conducted on lotus ecotypes and transgenic Arabidopsis (OE-<i>NnMKK4</i> and OE-<i>NnCYCD5</i>) confirmed that <i>NnMKK4</i> acts as a cold receptor and that <i>NnCYCD5</i> promotes cell cycle progression and growth under cold conditions.</li>\u0000 \u0000 \u0000 <li>Collectively, our findings provide novel insights into the co-evolutionary dynamics of epigenetic and genetic variations that are associated with different growth cycles of lotus ecotypes in response to cold stress.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"252 4","pages":"1510-1525"},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148983986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"David J. Beerling","authors":"","doi":"10.1111/nph.71468","DOIUrl":"10.1111/nph.71468","url":null,"abstract":"<p>David J. Beerling, University of Sheffield (UK).\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"252 4","pages":"1396-1398"},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.71468","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148809960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GCR6, a novel QTL regulating grain chalkiness in rice","authors":"Bo Cheng,&nbsp;Shoufa Wang,&nbsp;Guangqi Wang,&nbsp;Hongru Zhang,&nbsp;Yibo Li","doi":"10.1111/nph.71563","DOIUrl":"10.1111/nph.71563","url":null,"abstract":"<p>Isolation and characterization of GCR6 controlling grain chalkiness in rice.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"252 4","pages":"1366-1371"},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.71563","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148909155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought and salinity stress remodel Asian rice (Oryza sativa) leaf development through cell-type-specific regulatory programs","authors":"Sean M. Robertson,&nbsp;Obaid Maqsood,&nbsp;Chi Dang,&nbsp;Asher Pasha,&nbsp;Claudio Stasolla,&nbsp;Nicholas J. Provart,&nbsp;Olivia Wilkins","doi":"10.1111/nph.71378","DOIUrl":"10.1111/nph.71378","url":null,"abstract":"<p>Osmotic stressors impair tissue expansion and slow crop development, which can ultimately reduce yield. The mechanisms through which dividing and differentiating cells perceive, integrate, and respond to stress are not well understood. To explore this knowledge gap, we assessed Asian rice (<i>Oryza sativa</i>) seedling responses to drought and salinity stress by performing single-nucleus transcriptome analyses on developing leaves and by assaying ion distribution using ICP-MS and CoroNa Green staining. With a dataset of &gt; 125 000 high-quality nuclei, we identified transcriptome signatures of developmental trajectories for each of the major tissue systems, from their origin as dividing cells in the shoot apical meristem to mature cells in epidermal, mesophyll, and vascular tissues. We explored the extensive heterogeneity between cell types with respect to their responses to stress by developing an ensemble-based Perturbation Index and a tool for visualizing pseudobulk expression data and characterizing regulatory modules for different stress responses. Transcriptionally, moderate stress perturbs cells early in development and induces regulatory networks that inhibit cell division, whereas mild stressors primarily disrupt gene expression in differentiated tissues. Overall, these results indicate that leaf development occurring in salt and drought stress conditions is the product of a complex interplay of developmental, environmental, and cell-type contexts.</p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"252 4","pages":"1376-1395"},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.71378","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148372311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomics reveals lineage-associated structural variation and diversification in a barley fungal pathogen","authors":"Xiaohui Yu,&nbsp;Mumta Chhetri,&nbsp;Mafruha Hasan,&nbsp;Thomas H. Roberts,&nbsp;Peng Zhang,&nbsp;Robert F. Park,&nbsp;Yi Ding","doi":"10.1111/nph.71571","DOIUrl":"10.1111/nph.71571","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"252 4","pages":"1565-1583"},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.71571","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The PSI-NDH supercomplex prevents chilling-induced PSI photoinhibition","authors":"Ko Takeuchi,&nbsp;Shintaro Harimoto,&nbsp;Kentaro Ifuku","doi":"10.1111/nph.71594","DOIUrl":"10.1111/nph.71594","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Chilling stress induces photosystem I (PSI) photoinhibition in cucumber (<i>Cucumis sativus</i> L.). This process is associated with insufficient activity of the chloroplast NADH dehydrogenase-like complex (NDH). However, whether the importance of NDH can be generalized to other species and how NDH function is impaired remain unclear.</li>\u0000 \u0000 \u0000 <li>We first examined whether NDH is essential for PSI protection under chilling stress using an NDH-deficient rice (<i>Oryza sativa</i> L.) mutant.</li>\u0000 \u0000 \u0000 <li>The NDH-deficient rice exhibited pronounced PSI photoinhibition under chilling stress. By contrast, rice expressing flavodiiron protein (FLV), which functions as an alternative electron acceptor downstream of PSI, did not exhibit PSI photoinhibition under chilling stress, demonstrating that the electron sink capacity of NDH is important for PSI protection under chilling stress. Furthermore, in cucumber, chilling stress destabilizes the PSI-NDH supercomplex, leading to NDH monomerization and a consequent loss of NDH activity. This NDH monomerization is likely attributable to chilling-induced damage to the light-harvesting complex Lhca, which mediates the association between PSI and NDH.</li>\u0000 \u0000 \u0000 <li>Together, these results indicate that NDH protects PSI from photoinhibition under chilling stress, and that chilling-induced destabilization of the PSI-NDH supercomplex represents a key molecular mechanism underlying PSI over-reduction and photoinhibition in cucumber.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"252 4","pages":"1470-1487"},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149010112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordination of drought-adaptive root growth plasticity through auxin homeostasis by the ZmSPL12–ZmYUC5 module in maize","authors":"Hua Xu,&nbsp;Lei Ma,&nbsp;Qingyun Zhang,&nbsp;Zhigang Zheng,&nbsp;Jiajing Li,&nbsp;Sen Yang,&nbsp;Yanfeng Zhao,&nbsp;Lian Jin,&nbsp;Binbin Zhao,&nbsp;Zhijing Bai,&nbsp;Jiyuan Cao,&nbsp;Youpeng Tao,&nbsp;Junjie Zou,&nbsp;Baobao Wang,&nbsp;Shujing Cheng,&nbsp;Jinfang Chu,&nbsp;Jiafan Liu,&nbsp;Haiyang Wang,&nbsp;Miaoyun Xu","doi":"10.1111/nph.71592","DOIUrl":"10.1111/nph.71592","url":null,"abstract":"<p>\u0000 \u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"252 4","pages":"1526-1544"},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.71592","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149019814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A suppressor screen uncovers flu as a weak mutant and chloroplastic 1O2 triggers EX1-independent stress responses in Arabidopsis","authors":"Fan Zhang,&nbsp;张璠,&nbsp;Min Hu,&nbsp;Huan Zhao,&nbsp;Fengguang Wang,&nbsp;Mingxuan Li,&nbsp;Yu Zhang,&nbsp;Jiale Shi,&nbsp;Shiguo Chen,&nbsp;Jingrui Li,&nbsp;Liangsheng Wang,&nbsp;王良省","doi":"10.1111/nph.71279","DOIUrl":"10.1111/nph.71279","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Singlet oxygen (¹O₂) is the main reactive oxygen species produced in chloroplasts, particularly under environmental stress conditions. Studies using the Arabidopsis conditional <i>flu</i> (<i>flu1-1</i>) mutant have shown that chloroplastic ¹O₂ acts as a signal, activating retrograde signaling that eventually leads to photoinhibition or cell death.</li>\u0000 \u0000 \u0000 <li>Through a forward genetic screen for suppressors using ethyl methanesulfonate (EMS)-mutagenized Arabidopsis <i>flu1-1/ex1</i> mutants, we isolated two suppressor mutants (<i>mut1</i> and <i>mut2</i>) that restored ¹O₂-induced stress responses. Using combined methods of map-based cloning and high-throughput sequencing, we mapped the causal mutations in both <i>mut1</i> and <i>mut2</i> to the <i>FLUORESCENT</i> (<i>FLU</i>) gene again.</li>\u0000 \u0000 \u0000 <li>We verified that the widely used <i>flu1-1</i> is not the long-recognized null mutant, but rather a weak allele, encoding a variant that binds weakly to GluTR1 and leads to partial shutdown of tetrapyrrole biosynthesis in the dark. We further found that the <i>FLU</i>-knockout mutants produce more ¹O₂, activating transcriptional changes, enzymatic lipid peroxidation, and programmed cell death (PCD) even in the absence of EX1. Overexpression of SAFE1 suppressed ¹O₂-induced PCD in <i>mut2</i> in a dosage-dependent manner.</li>\u0000 \u0000 \u0000 <li>Collectively, our findings redefine the nature of the widely used <i>flu1-1</i> mutant and reveal that excessive ¹O₂ can overwhelm the inhibitory effect of endogenous SAFE1 and induce stress responses independently of EX1.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"252 4","pages":"1488-1509"},"PeriodicalIF":8.7,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147989691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}