New Phytologist最新文献

{"title":"Transgene-free genome editing in poplar","authors":"Lennart Hoengenaert, Chantal Anders, Jan Van Doorsselaere, Ruben Vanholme, Wout Boerjan","doi":"10.1111/nph.20415","DOIUrl":"https://doi.org/10.1111/nph.20415","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>Precise gene-editing methods are valuable tools to enhance genetic traits. Gene editing is commonly achieved via stable integration of a gene-editing cassette in the plant's genome. However, this technique is unfavorable for field applications, especially in vegetatively propagated plants, such as many commercial tree species, where the gene-editing cassette cannot be segregated away without breaking the genetic constitution of the elite variety.</li>\u0000<li>Here, we describe an efficient method for generating gene-edited <i>Populus tremula × P. alba</i> (poplar) trees without incorporating foreign DNA into its genome. Using <i>Agrobacterium tumefaciens</i>, we expressed a base-editing construct targeting <i>CCoAOMT1</i> along with the <i>ALS</i> genes for positive selection on a chlorsulfuron-containing medium.</li>\u0000<li>About 50% of the regenerated shoots were derived from transient transformation and were free of T-DNA. Overall, 7% of the chlorsulfuron-resistant shoots were T-DNA free, edited in the <i>CCoAOMT1</i> gene and nonchimeric.</li>\u0000<li>Long-read whole-genome sequencing confirmed the absence of any foreign DNA in the tested gene-edited lines. Additionally, we evaluated the <i>CodA</i> gene as a negative selection marker to eliminate lines that stably incorporated the T-DNA into their genome. Although the latter negative selection is not essential for selecting transgene-free, gene-edited <i>Populus tremula × P. alba</i> shoots, it may prove valuable for other genotypes or varieties.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"103 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142992030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell proliferation suppressor RBR1 interacts with ARID1 to promote pollen mitosis via stabilizing DUO1 in Arabidopsis","authors":"Lei Li, Qianqian Hu, Yi Zhao, Ting Jiang, Huaihao Yang, Binglian Zheng","doi":"10.1111/nph.20399","DOIUrl":"https://doi.org/10.1111/nph.20399","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>In plants, sperm cell formation involves two rounds of pollen mitoses, in which the microspore initiates the first pollen mitosis (PMI) to produce a vegetative cell and a generative cell, then the generative cell continues the second mitosis (PMII) to produce two sperm cells. DUO1, a R2R3 Myb transcription factor, is activated in the generative cell to promote S-G2/M transition during PMII. Loss-of-function of DUO1 caused a complete arrest of PMII. Despite the importance of DUO1, how DUO1 is regulated is largely unexplored.</li>\u0000<li>We previously demonstrated that ARID1, an ARID transcription factor, stimulates <i>DUO1</i> transcription.</li>\u0000<li>Here, we show that cell proliferation suppressor RBR1 interacts with ARID1 to stabilize DUO1. While the C-terminus of RBR1 is dispensable for vegetative growth, it plays a crucial role in reproductive development and facilitates interaction with ARID1. Moreover, DUO1 is a short-lived protein, ARID1 promotes the RBR1–DUO1 interaction, and RBR1 stabilizes DUO1 in a proteasome-dependent manner. Thus, RBR1 promotes DUO1-dependent PMII progression via antagonizing its repressive role in the cell cycle factors CDKA;1 and CYCB1;1.</li>\u0000<li>Collectively, we uncover that ARID1 and RBR1 act in concert to regulate DUO1 at both the transcriptional and posttranscriptional levels, balancing cell specification and cell division.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"45 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apoplastic pH is a chemical switch for extracellular H2O2 signaling in abscisic acid-mediated inhibition of cotyledon greening","authors":"Miao Zhou, Jia Yuan Ye, Yi Ju Shi, Yi Jie Jiang, Yao Zhuang, Qing Yang Zhu, Xing Xing Liu, Zhong Jie Ding, Shao Jian Zheng, Chong Wei Jin","doi":"10.1111/nph.20400","DOIUrl":"https://doi.org/10.1111/nph.20400","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>The apoplastic pH (pH_Apo) in plants is susceptible to environmental stimuli. However, the biological implications of pH_Apo variation have remained largely unknown.</li>\u0000<li>The universal stress phytohormone abscisic acid (ABA) as well as the major environmental stimuli drought and salinity were selected as representative cases to investigate how changes in pH_Apo relate to plant behaviors in <i>Arabidopsis</i>. Variations in pH_Apo negatively regulated the cotyledon greening inhibition to the universal stress hormone ABA or environmental stimuli through the action of extracellular hydrogen peroxide (eH₂O₂).</li>\u0000<li>Further studies revealed that an increase in pH_Apo diminishes the chemical reactivity of eH₂O₂, effectively functioning as an ‘off’ switch for its action in oxidizing thiols of plasma membrane proteins. Consequently, this suppresses the eH₂O₂-mediated cotyledon greening inhibition to environmental stimuli and ABA, alongside inhibiting the eH₂O₂-mediated intracellular Ca²⁺ signaling. Conversely, a decrease in pH_Apo serves as an ‘on’ switch for the action of eH₂O₂.</li>\u0000<li>In summary, the pH_Apo is a crucial messenger and chemical switch for eH₂O₂ in signal transduction, notwithstanding the apparent simplicity of the underlying mechanism. Our findings provide a novel fundamental biological insight into the significance of pH.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"44 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concurrent common fungal networks formed by different guilds of fungi","authors":"Matthias C. Rillig, Anika Lehmann, Ian R. Mounts, Beatrice M. Bock","doi":"10.1111/nph.20418","DOIUrl":"https://doi.org/10.1111/nph.20418","url":null,"abstract":"&lt;h2&gt; Introduction&lt;/h2&gt;\u0000&lt;p&gt;Networks formed by fungi that link among plants have captured the imagination of scientists and the wider public alike (Selosse &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2006&lt;/span&gt;; Karst &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;). This work on fungal connections among plant roots has almost exclusively focused on mycorrhizal fungi, with most work focusing on arbuscular mycorrhizal and ectomycorrhizal fungi; other groups of mycorrhiza, such as ericoid mycorrhiza and orchid mycorrhiza have also been studied. Reasons underpinning this focus on common mycorrhizal networks (CMNs) are quite evident: these fungi form well-documented and functionally relevant symbioses with the majority of plants and the fungi grow inside the roots, forming symbiotic exchange interfaces (Smith &amp; Read, &lt;span&gt;2008&lt;/span&gt;).&lt;/p&gt;\u0000&lt;p&gt;A recently introduced conceptual framework (Rillig &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2025&lt;/span&gt;) has proposed a hierarchical set of terms to describe such links: the current definition of common mycorrhizal networks demands the presence of hyphal continuous links that forms an uninterrupted cytoplasmic flow between roots of at least two plants (Karst &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;). This is a special case, in reality, for which several criteria have to be fulfilled (Lehmann &amp; Rillig, &lt;span&gt;2025&lt;/span&gt;) to ensure that it is just the resource transfer via the hyphal link that is responsible for any measured plant responses. In the new framework, this special case is referred to as common mycorrhizal networks with hyphal continuity (CMN-HC). In this conceptual framework, common mycorrhizal networks of any kind – involving direct hyphal connections or not – are referred to as CMNs. In addition, the term common fungal network (CFN) has been introduced, representing the most general case of hyphal linkages among plants: those that are formed by any type of filamentous fungus (not limited to mycorrhizal fungi) and that are either direct or indirect in their mode of linking (i.e. hyphal continuity or not).&lt;/p&gt;\u0000&lt;p&gt;A systematic mapping of the field of ‘common mycorrhizal networks’ revealed that &lt;i&gt;c&lt;/i&gt;. 33% of the experimental research data is on networks formed not just by the targeted mycorrhizal fungi, but with other filamentous fungi present in addition to mycorrhizal fungi (Lehmann &amp; Rillig, &lt;span&gt;2025&lt;/span&gt;). These are mainly field studies or studies using whole microbial communities as inoculum sources for the network. Thus, effects of CFNs are already implicitly part of our experimental results, but we do not know about their contribution to the studied mycorrhizal networks. We propose here that such CFNs are likely the reality in soils, rather than just CMNs, and that this more complex reality should be captured in future work on fungal networks linking among plants (Fig. 1). In this paper, we build on the recent conceptual development and these systematic mapping results to propose research on various forms of CFNs.&lt;/p&gt;\u0000&lt;figure&gt;&lt;picture&gt;\u0000&lt;source","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"18 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Charlotte Grossiord","authors":"","doi":"10.1111/nph.20404","DOIUrl":"10.1111/nph.20404","url":null,"abstract":"&lt;p&gt;I have always been passionate about plant science. I think it all started with my upbringing in Africa. I grew up in different countries with climates ranging from tropical to semi-arid, so I was constantly surrounded by nature and exposed to an incredible variety of ecosystems. It was impossible not to fall in love with the diversity of plant life. As a kid, I spent my days running through forests, climbing trees, and building treehouses. I was in awe of how resilient and beautiful the natural world was. And it wasn't just outdoors – our home was always filled with plants. They were't just decorations to me; they felt like part of the family, living companions that brought life into every room. All of that shaped my curiosity about plants – how they work, how they interact with their surroundings, and why they are so vital to our world. That curiosity eventually led me to pursue a PhD, where I focused on understanding the role of biodiversity in forests and how it influences how they function. Now, as a plant ecophysiologist, I study how forests respond to climate change and how biodiversity helps them stay resilient. That childhood wonder I felt has not gone away – it still drives everything I do and makes me excited to share my love for plants with others.&lt;/p&gt;&lt;p&gt;Deciding to pursue a career in research was't something that happened overnight, but once I figured it out, it felt like the perfect fit. I have always had a passion for science and a fascination with understanding the natural world, but when I started university, I honestly did't know what researchers or scientists actually did. That all changed during my first research internships. Those experiences gave me a glimpse into the life of a scientist. It didn't take long for me to realize this was exactly what I wanted to do. I loved the variety the job offered – spending time in the field, working in the lab, coming up with new ideas, and traveling to conferences where I got to meet people from all over the world. One of the things that surprised me was how much I enjoyed the writing side of it. Writing had always been a passion of mine, and being able to craft papers and create figures to tell scientific stories was so rewarding. What really sealed the deal for me was the chance to piece together puzzles about how forests function and respond to climate change. The mix of creativity, curiosity, and the opportunity to make a difference felt irresistible. Even now, I still feel that same excitement. Research is a job where no two days are the same, and that is exactly what makes it so fulfilling.&lt;/p&gt;&lt;p&gt;What gets me excited every day is the chance to work with an incredible team of scientists, some of whom are just starting their scientific journey. Mentoring them and helping them build their careers in science is honestly one of the best parts of what I do. Whether we are tackling research challenges together or celebrating their achievements, it is so rewarding to see their confidence","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 5","pages":"1843-1845"},"PeriodicalIF":8.3,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aging-dependent temporal regulation of MIR156 epigenetic silencing by CiLDL1 and CiNF-YB8 in chrysanthemum","authors":"Xuekai Gao,&nbsp;Lei Liu,&nbsp;Tianle Wang,&nbsp;Chuyan Jiang,&nbsp;Yujin Xue,&nbsp;Yahui Sun,&nbsp;Zhaoyu Gu,&nbsp;Yanjie Xu,&nbsp;Cai-Zhong Jiang,&nbsp;Junping Gao,&nbsp;Bo Hong,&nbsp;Chao Ma","doi":"10.1111/nph.20354","DOIUrl":"10.1111/nph.20354","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Temporal decline in microRNA miR156 expression is crucial for the transition to, and maintenance of, the adult phase and flowering competence in flowering plants. However, the molecular mechanisms underlying the temporal regulation of miR156 reduction remain largely unknown.</li>\u0000 \u0000 <li>Here, we investigated the epigenetic mechanism regulating the temporal silencing of <i>cin-MIR156</i> in wild chrysanthemum (<i>Chrysanthemum indicum</i>), focusing on the role of the lysine-specific demethylase CiLDL1 and the nuclear factor Y complex.</li>\u0000 \u0000 <li>CiLDL1 and CiNF-YB8 interact with the classical histone-like fold domain (HFD) of CiNF-YC1 and CiNF-YA3, which form distinct heterotrimers binding to the ‘CCAAT’ box in the promoter region of <i>cin-MIR156ab</i>. CiLDL1 and CiNF-YB8 have opposing effects on <i>cin-MIR156ab</i> expression, with influencing histone 3 lysine 4 demethylation (H3K4me2) levels at the <i>cin-MIR156ab</i> locus. During aging, decreased <i>CiNF-YB8</i> expression leads to a quantitative switch from the CiNF-YA3–CiNF-YC1–CiNF-YB8 heterotrimer to the CiNF-YA3–CiNF-YC1–CiLDL1 heterotrimer, which reduces H3K4me2 levels at the <i>cin-MIR156ab</i> locus, thus temporal silencing its expression.</li>\u0000 \u0000 <li>Our results thus reveal that the dynamic regulatory shift between CiLDL1 and CiNF-YB8 ensures proper aging-dependent flowering in chrysanthemum.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 5","pages":"2309-2321"},"PeriodicalIF":8.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two reductases complete steroidal glycoalkaloids biosynthesis in potato","authors":"Ryota Akiyama, Daiki Terami, Aozora Noda, Bunta Watanabe, Naoyuki Umemoto, Toshiya Muranaka, Kazuki Saito, Yukihiro Sugimoto, Masaharu Mizutani","doi":"10.1111/nph.20411","DOIUrl":"https://doi.org/10.1111/nph.20411","url":null,"abstract":"&lt;h2&gt; Introduction&lt;/h2&gt;\u0000&lt;p&gt;Steroidal glycoalkaloids (SGAs), a class of specialized metabolites derived from cholesterol and containing nitrogen, are typically found in the Solanaceae plant family. This includes staple food crops such as potato (&lt;i&gt;Solanum tuberosum&lt;/i&gt;), tomato (&lt;i&gt;Solanum lycopersicum&lt;/i&gt;), and eggplant (&lt;i&gt;Solanum melongena&lt;/i&gt;) (Friedman, &lt;span&gt;2002&lt;/span&gt;, &lt;span&gt;2006&lt;/span&gt;, &lt;span&gt;2015&lt;/span&gt;). SGAs serve as a chemical protectant against a wide range of plant pathogens and herbivores (Milner &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2011&lt;/span&gt;). Some SGAs are considered antinutritional factors in foods, causing gastrointestinal and neurological disorders in humans, in addition to exhibiting unpleasant tastes described as bitter, burning, scratchy, or acrid.&lt;/p&gt;\u0000&lt;p&gt;SGAs are composed of two structural elements: the aglycone unit, an anitrogen-containing steroid derived from cholesterol, and a glycoside residue attached to the hydroxy group at C-3 (Milner &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2011&lt;/span&gt;; Sawai &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2014&lt;/span&gt;). The skeletal structure of the aglycone allows SGAs to be classified into two general classes: spirosolane and solanidane (Friedman &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;1997&lt;/span&gt;; Milner &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2011&lt;/span&gt;). Tomatoes exclusively contain spirosolane-type SGAs, with α-tomatine and dehydrotomatine being the most abundant in green tissues (Friedman, &lt;span&gt;2002&lt;/span&gt;). Conversely, potatoes produce solanidane-type SGAs, specifically α-solanine and α-chaconine, which constitute over 90% of the total SGAs in cultivated potatoes (Shakya &amp; Navarre, &lt;span&gt;2008&lt;/span&gt;).&lt;/p&gt;\u0000&lt;p&gt;Recent research on tomato and potato plants has uncovered numerous biosynthetic genes involved in SGA biosynthesis (Fig. 1). Three cytochrome P450 monooxygenases (PGA2/GAME6, PGA1/GAME8, and PGA3/GAME4), a 2-oxoglutarate-dependent dioxygenase (16DOX/GAME11), and an aminotransferase (PGA4/GAME12) have been identified as key players in the early stages of SGA biosynthesis from cholesterol (Itkin &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2013&lt;/span&gt;; Umemoto &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2016&lt;/span&gt;; Nakayasu &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2017&lt;/span&gt;, &lt;span&gt;2021&lt;/span&gt;). Additionally, GAME15, a member of the cellulose synthase-like family, has been identified as an SGA biosynthetic gene (Jozwiak &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;). GAME15 catalyzes the transglucuronidation of the C3-hydroxy group of cholesterol, which represents the first step in the SGA biosynthetic pathway from cholesterol (Jozwiak &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;). Furthermore, GAME15 functions as a scaffold protein, facilitating physical interactions among the SGA biosynthetic enzymes and enabling efficient substrate channeling (Boccia &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;; Jozwiak &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;). The final products, such as α-solanine and α-tomatine, do not retain the glucuronic acid moiety, suggesting that the glucuronic acid is hydrolyzed at some point in the biosynthetic pathway, although the exact timing of this r","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"6 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the subdaily variations in methane, nitrous oxide and carbon dioxide fluxes from upland tropical tree stems","authors":"Laëtitia M. Bréchet, Roberto L. Salomόn, Katerina Machacova, Clément Stahl, Benoît Burban, Jean‐Yves Goret, Kathy Steppe, Damien Bonal, Ivan A. Janssens","doi":"10.1111/nph.20401","DOIUrl":"https://doi.org/10.1111/nph.20401","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Recent studies have shown that stem fluxes, although highly variable among trees, can alter the strength of the methane (CH<jats:sub>4</jats:sub>) sink or nitrous oxide (N<jats:sub>2</jats:sub>O) source in some forests, but the patterns and magnitudes of these fluxes remain unclear. This study investigated the drivers of subdaily and seasonal variations in stem and soil CH<jats:sub>4</jats:sub>, N<jats:sub>2</jats:sub>O and carbon dioxide (CO<jats:sub>2</jats:sub>) fluxes.</jats:list-item> <jats:list-item>CH<jats:sub>4</jats:sub>, N<jats:sub>2</jats:sub>O and CO<jats:sub>2</jats:sub> fluxes were measured continuously for 19 months in individual stems of two tree species, <jats:italic>Eperua falcata</jats:italic> (Aubl.) and <jats:italic>Lecythis poiteaui</jats:italic> (O. Berg), and surrounding soils using an automated chamber system in an upland tropical forest. Subdaily variations in these fluxes were related to environmental and stem physiological (sap flow and stem diameter variations) measurements under contrasting soil water conditions.</jats:list-item> <jats:list-item>The results showed that physiological and climatic drivers only partially explained the subdaily flux variations. Stem CH<jats:sub>4</jats:sub> and CO<jats:sub>2</jats:sub> emissions and N<jats:sub>2</jats:sub>O uptake varied with soil water content, time of day and between individuals. Stem fluxes decoupled from soil fluxes.</jats:list-item> <jats:list-item>Our study contributes to understanding the regulation of stem greenhouse gas fluxes. It suggests that additional variables (e.g. internal gas concentrations, wood‐colonising microorganisms, wood density and anatomy) may account for the remaining unexplained variability in stem fluxes, highlighting the need for further studies.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"16 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142987454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"At least two functions for BdMUTE during the development of stomatal complexes in Brachypodium distachyon","authors":"Laura Serna","doi":"10.1111/nph.20396","DOIUrl":"https://doi.org/10.1111/nph.20396","url":null,"abstract":"","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"46 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The aerial epidermis is a major site of quinolizidine alkaloid biosynthesis in narrow-leafed lupin","authors":"Karen Michiko Frick,&nbsp;Marcus Daniel Brandbjerg Bohn Lorensen,&nbsp;Nikola Micic,&nbsp;Eddi Esteban,&nbsp;Asher Pasha,&nbsp;Alexander Schulz,&nbsp;Nicholas James Provart,&nbsp;Hussam Hassan Nour-Eldin,&nbsp;Nanna Bjarnholt,&nbsp;Christian Janfelt,&nbsp;Fernando Geu-Flores","doi":"10.1111/nph.20384","DOIUrl":"10.1111/nph.20384","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 5","pages":"2052-2068"},"PeriodicalIF":8.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142974547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}