New Phytologist最新文献

{"title":"A barley MLA immune receptor is activated by a fungal nonribosomal peptide effector for disease susceptibility","authors":"Yueqiang Leng, Florian Kümmel, Mingxia Zhao, István Molnár, Jaroslav Doležel, Elke Logemann, Petra Köchner, Pinggen Xi, Shengming Yang, Matthew J. Moscou, Jason D. Fiedler, Yang Du, Burkhard Steuernagel, Steven Meinhardt, Brian J. Steffenson, Paul Schulze-Lefert, Shaobin Zhong","doi":"10.1111/nph.20289","DOIUrl":"https://doi.org/10.1111/nph.20289","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>The barley <i>Mla</i> locus contains functionally diversified genes that encode intracellular nucleotide-binding leucine-rich repeat receptors (NLRs) and confer strain-specific immunity to biotrophic and hemibiotrophic fungal pathogens.</li>\u0000<li>In this study, we isolated a barley gene <i>Scs6</i>, which is an allelic variant of <i>Mla</i> genes but confers susceptibility to the isolate ND90Pr (<i>Bs</i>_ND90Pr) of the necrotrophic fungus <i>Bipolaris sorokiniana</i>. We generated <i>Scs6</i> transgenic barley lines and showed that <i>Scs6</i> is sufficient to confer susceptibility to <i>Bs</i>_ND90Pr in barley genotypes naturally lacking the receptor. The <i>Scs6-</i>encoded NLR (SCS6) is activated by a nonribosomal peptide (NRP) effector produced by <i>Bs</i>_ND90Pr to induce cell death in barley and <i>Nicotiana benthamiana</i>. Domain swaps between MLAs and SCS6 reveal that the SCS6 leucine-rich repeat domain is a specificity determinant for receptor activation by the NRP effector.</li>\u0000<li><i>Scs6</i> is maintained in both wild and domesticated barley populations. Our phylogenetic analysis suggests that <i>Scs6</i> is a <i>Hordeum</i>-specific innovation.</li>\u0000<li>We infer that SCS6 is a <i>bona fide</i> immune receptor that is likely directly activated by the nonribosomal peptide effector of <i>Bs</i>_ND90Pr for disease susceptibility in barley. Our study provides a stepping stone for the future development of synthetic NLR receptors in crops that are less vulnerable to modification by necrotrophic pathogens.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"81 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anionic phospholipid-mediated transmembrane transport and intracellular membrane trafficking in plant cells","authors":"Qun Zhang, Like Shen, Feng Lin, Qi Liao, Shi Xiao, Wenhua Zhang","doi":"10.1111/nph.20329","DOIUrl":"https://doi.org/10.1111/nph.20329","url":null,"abstract":"Cellular membranes primarily consist of proteins and lipids. These proteins perform cellular functions such as metabolic regulation, environmental and hormonal signal sensing, and nutrient transport. There is increasing experimental evidence that certain lipids, particularly anionic phospholipids, can act as signaling molecules. Specific examples of functional regulation by anionic phospholipids in plant cells have been reported for transporters, channels, and even receptors. By regulating the structure and activity of membrane-integral proteins, these phospholipids mediate the transport of phytohormones and ions, and elicit physiological responses to developmental and environmental cues. Phospholipids also control membrane protein abundance and lipid composition and abundance by facilitating vesicular trafficking. In this review, we discuss recent research that elucidates the mechanisms by which membrane-integral transporters and channels are controlled via phospholipid signaling, as well as the regulation of membrane protein accumulation by phospholipids through coordinated removal, recycling, and degradation processes.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"20 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The SLR1-OsMADS23-D14 module mediates the crosstalk between strigolactone and gibberellin signaling to control rice tillering","authors":"Xingxing Li, Zizhao Xie, Tian Qin, Chenghang Zhan, Liang Jin, Junli Huang","doi":"10.1111/nph.20331","DOIUrl":"https://doi.org/10.1111/nph.20331","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>Strigolactones (SLs) and gibberellins (GAs) have been found to inhibit plant branching or tillering, but molecular mechanisms underlying the interplay between SL and GA signaling to modulate tillering remain elusive.</li>\u0000<li>We found that the transcription factor OsMADS23 plays a crucial role in the crosslink between SL and GA signaling in rice tillering. Loss-of-function mutant <i>osmads23</i> shows normal axillary bud formation but defective bud outgrowth, thus reducing the tiller number in rice, whereas overexpression of <i>OsMADS23</i> significantly increases tillering by promoting tiller bud outgrowth.</li>\u0000<li>OsMADS23 physically interacts with DELLA protein SLENDER RICE1 (SLR1), and the interaction reciprocally stabilizes each other. Genetic evidence showed that SLR1 is required for OsMADS23 to control rice tillering. OsMADS23 acts as an upstream transcriptional repressor to inhibit the expression of SL receptor gene <i>DWARF14</i> (<i>D14</i>), and addition of SLR1 further enhances OsMADS23-mediated transcriptional repression of <i>D14</i>, indicating that <i>D14</i> is the downstream target gene of OsMADS23–SLR1 complex. Moreover, application of exogenous SL and GA reduces the protein stability of OsMADS23–SLR1 complex and promotes <i>D14</i> expression.</li>\u0000<li>Our results revealed that SLs and GAs synergistically inhibit rice tillering by destabilizing OsMADS23–SLR1 complex, which provides important insights into the molecular networks of SL–GA synergistic interaction during rice tillering.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"33 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The promising role of proteomes and metabolomes in defining the single‐cell landscapes of plants","authors":"Christopher R. Anderton, R. Glen Uhrig","doi":"10.1111/nph.20303","DOIUrl":"https://doi.org/10.1111/nph.20303","url":null,"abstract":"SummaryThe plant community has a strong track record of RNA sequencing technology deployment, which combined with the recent advent of spatial platforms (e.g. 10× genomics) has resulted in an explosion of single‐cell and nuclei datasets that can be positioned in an <jats:italic>in situ</jats:italic> context within tissues (e.g. a cell atlas). In the genomics era, application of proteomics technologies in the plant sciences has always trailed behind that of RNA sequencing technologies, largely due in part to upfront cost, ease‐of‐use, and access to expertise. Conversely, the use of early analytical tools for characterizing small molecules (metabolites) from plant systems predates nucleic acid sequencing and proteomics analysis, as the search for plant‐based natural products has played a significant role in improving human health throughout history. As the plant sciences field now aims to fully define cell states, cell‐specific regulatory networks, metabolic asymmetry and phenotypes, the measurement of proteins and metabolites at the single‐cell level will be paramount. As a result of these efforts, the plant community will unlock exciting opportunities to accelerate discovery and drive toward meaningful translational outcomes.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"222 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic regulation of lignin biosynthesis in wood formation","authors":"Hongyan Ma, Liwei Su, Wen Zhang, Yi Sun, Danning Li, Shuang Li, Ying-Chung Jimmy Lin, Chenguang Zhou, Wei Li","doi":"10.1111/nph.20328","DOIUrl":"https://doi.org/10.1111/nph.20328","url":null,"abstract":"&lt;h2&gt; Introduction&lt;/h2&gt;\u0000&lt;p&gt;Lignin is a phenolic polymer and a major component of wood, constituting &lt;i&gt;c&lt;/i&gt;. 20–30% of the wood biomass. It is the key limiting factor for wood conversion efficiency because it must be removed to extract the other two wood components, cellulose (40–50% of wood) and hemicelluloses (20–30% of wood), for paper making (Sarkanen, &lt;span&gt;1976&lt;/span&gt;; Chiang, &lt;span&gt;2002&lt;/span&gt;; Ragauskas &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2006&lt;/span&gt;). Wood cellulose and hemicelluloses are potentially commercial feedstock for biofuel production (Sarkanen, &lt;span&gt;1976&lt;/span&gt;). Lignin is synthesized through the polymerization of three canonical monolignols, &lt;i&gt;p&lt;/i&gt;-coumaryl, coniferyl, and sinapyl alcohols, known as the &lt;i&gt;p&lt;/i&gt;-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) units, respectively (Freudenberg, &lt;span&gt;1965&lt;/span&gt;; Sarkanen &amp; Ludwig, &lt;span&gt;1971&lt;/span&gt;; Higuchi, &lt;span&gt;1997&lt;/span&gt;). Monolignols are biosynthesized from phenylalanine through a complex network pathway mediated by &gt; 20 pathway enzymes. The mediation requires combined catalysis functions of individual enzymes (Higuchi, &lt;span&gt;1997&lt;/span&gt;; Sulis &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;; Li &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;), enzyme complexes (Chen &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2011&lt;/span&gt;, &lt;span&gt;2014&lt;/span&gt;; Lin &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2015&lt;/span&gt;; Yan &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2018&lt;/span&gt;; X. Zhao &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;), and protein phosphorylation (Wang &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2015&lt;/span&gt;) to produce monolignols for lignin polymerization.&lt;/p&gt;\u0000&lt;p&gt;At the genetic level, the expression of genes encoding pathway enzymes controls monolignol production, affecting lignin biosynthesis and thus the recalcitrance of wood to conversion. Transregulation of monolignol gene expression has been extensively studied. Approximately 50 transcription factors (TFs) belonging to MYB, NAC, WRKY, ERF, WBLH, TZF, HSF, MADS-box, and LBD families have been implicated in such regulation (Supporting Information Table S1; Li &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;). Only a few TFs, such as PtoMYB221, PtrMYB092, PtrMYB161, PtrMYB189, PdMYB221/LTF1, BpNAC012, PtrHSFB3-1, and PagERF81, have been demonstrated to directly regulate monolignol biosynthesis genes (including &lt;i&gt;COMT2&lt;/i&gt;, &lt;i&gt;CAld5H1&lt;/i&gt;, &lt;i&gt;CAld5H2&lt;/i&gt;, &lt;i&gt;CCoAOMT2&lt;/i&gt;, &lt;i&gt;CCoAOMT1&lt;/i&gt;, &lt;i&gt;4CL&lt;/i&gt;5, &lt;i&gt;C3H3&lt;/i&gt;, &lt;i&gt;C4H1&lt;/i&gt;, &lt;i&gt;PAL2&lt;/i&gt;, &lt;i&gt;PAL4&lt;/i&gt;, &lt;i&gt;PAL5&lt;/i&gt;, &lt;i&gt;CSE1&lt;/i&gt;, &lt;i&gt;4CL1&lt;/i&gt;, &lt;i&gt;CCR2&lt;/i&gt;, and &lt;i&gt;CCR1&lt;/i&gt;) (Tang &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2015&lt;/span&gt;; Gui &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2019&lt;/span&gt;; H. Chen &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2019&lt;/span&gt;; Hu &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2019&lt;/span&gt;; Wang &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2020&lt;/span&gt;; Liu &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2021&lt;/span&gt;; X. W. Zhao &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;). These TFs bind to the promoters of monolignol genes and interact with other regulators to exert activating or repressive effects on lignin biosynthesis.&lt;/p&gt;\u0000&lt;p&gt;Gene expression can be controlled by histone modifications, including acetylation and methylation (Grunstein, &lt;span&gt;1997&lt;/s","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"66 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-activated channelrhodopsins: a revolutionary toolkit for the remote control of plant signalling","authors":"Rainer Hedrich, Matthew Gilliham","doi":"10.1111/nph.20311","DOIUrl":"https://doi.org/10.1111/nph.20311","url":null,"abstract":"Channelrhodopsins (CHRs), originating within algae and protists, are membrane-spanning ion channel proteins that are directly activated and/or deactivated by specific wavelengths of light. Since 2005, CHRs have been deployed as genetically encoded optogenetic tools to rapidly advance understanding of neuronal networks. CHRs provide the opportunity to finely tune ion transport across membranes and regulate membrane potential. These are fundamental biochemical signals, which in plants can be translated into physiological and developmental responses such as changes in photosynthesis, growth, turgor, vascular hydraulics, phosphorylation or reactive oxygen species (ROS) status, gene expression, or even cell death. Exploration of CHR family diversity and structure–function engineering has led to the expansion of the CHR optogenetic toolbox, offering unparalleled opportunities to precisely control and understand electrical and secondary messenger signalling in higher plants. In this Tansley Insight, we provide an overview of the recent progress in the application of CHR optogenetics in higher plants and discuss their possible uses in the remote control of plant biology, illuminating a new future domain for plant research enabled through synthetic biology.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"1 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142777436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular mycorrhizal interactions and nutrient supply mediate floral trait variation and pollinator visitation","authors":"Aidee Guzman,&nbsp;Marisol Montes,&nbsp;Nada Lamie,&nbsp;Martin Bañuelos,&nbsp;Gisel DeLaCerda,&nbsp;Isabel Soria-Gilman,&nbsp;Mary Firestone,&nbsp;Timothy Bowles,&nbsp;Claire Kremen","doi":"10.1111/nph.20219","DOIUrl":"10.1111/nph.20219","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 1","pages":"406-419"},"PeriodicalIF":8.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20219","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maarja Öpik to take up the position of New Phytologist Editor-in-Chief from January 2025","authors":"Keith Lindsey","doi":"10.1111/nph.20305","DOIUrl":"10.1111/nph.20305","url":null,"abstract":"<p>The New Phytologist Foundation is delighted to announce that Professor Maarja Öpik will take up the position of Editor-in-Chief of <i>New Phytologist</i> from January 2025, for an initial term of 5 years.</p><p>Maarja has served as a member of <i>New Phytologist</i>'s editorial board since 2013 and is a professor of Molecular Ecology and Director of the Institute of Ecology and Earth Sciences at the Faculty of Science and Technology at the University of Tartu, Estonia.</p><p>Maarja's research addresses the interactions between plants and mycorrhizal fungi, with specific focus on arbuscular mycorrhizal fungal diversity patterns. Maarja pioneered one of the first public databases in the field, MaarjAM (Öpik <i>et al</i>., <span>2010</span>), which is now widely used as a tool for arbuscular mycorrhizal fungal identification and in arbuscular mycorrhizal fungus ecological research.</p><p>Maarja will lead an outstanding international board of Editors that focuses on all aspects of plant biology, spanning the journal's five sections: Physiology &amp; Development, Interaction, Environment, Evolution, and Transformative Plant Biotechnology. Maarja noted ‘<i>New Phytologist</i> is a journal that inspires its readers and authors, and this is the main quality that I aspire to keep, strengthen and develop as an Editor-in-Chief. Publishing inspiring papers and maintaining an active, engaging scientific community contributes towards strengthening the journal and its community’.</p><p>Maarja will take over the position from Professor Alistair M. Hetherington, who will step down as Editor-in-Chief after 12 years of outstanding service. We are grateful for the outstanding leadership offered by Alistair Hetherington, and the journal's achievements under his tenure as Editor-in-Chief are many. As we look forward, I am delighted to welcome Maarja, an exemplary scientist and editorial colleague to the position of Editor-in-Chief. We are excited to support Maarja's vision for the journal, and her commitment to the ethos of the Foundation in promoting plant science and supporting the international community of plant biologists. We look forward to the next chapter of the journal's development under Maarja's leadership.</p><p>The New Phytologist Foundation remains neutral with regard to jurisdictional claims in maps and in any institutional affiliations.</p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 1","pages":"5"},"PeriodicalIF":8.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142777426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decades-old carbon reserves are widespread among tree species, constrained only by sapwood longevity","authors":"Drew M. P. Peltier, Mariah S. Carbone, Kiona Ogle, George W. Koch, Andrew D. Richardson","doi":"10.1111/nph.20310","DOIUrl":"https://doi.org/10.1111/nph.20310","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>Carbon reserves are distributed throughout plant cells allowing past photosynthesis to fuel current metabolism. In trees, comparing the radiocarbon (Δ¹⁴C) of reserves to the atmospheric bomb spike can trace reserve ages.</li>\u0000<li>We synthesized Δ¹⁴C observations of stem reserves in nine tree species, fitting a new process model of reserve building. We asked how the distribution, mixing, and turnover of reserves vary across trees and species. We also explored how stress (drought and aridity) and disturbance (fire and bark beetles) perturb reserves.</li>\u0000<li>Given sufficient sapwood, young (&lt; 1 yr) and old (20–60+ yr) reserves were simultaneously present in single trees, including ‘prebomb’ reserves in two conifers. The process model suggested that most reserves are deeply mixed (30.2 ± 21.7 rings) and then respired (2.7 ± 3.5-yr turnover time). Disturbance strongly increased Δ¹⁴C mean ages of reserves (+15–35 yr), while drought and aridity effects on mixing and turnover were species-dependent. Fire recovery in <i>Sequoia sempervirens</i> also appears to involve previously unobserved outward mixing of old reserves.</li>\u0000<li>Deep mixing and rapid turnover indicate most photosynthate is rapidly metabolized. Yet ecological variation in reserve ages is enormous, perhaps driven by stress and disturbance. Across species, maximum reserve ages appear primarily constrained by sapwood longevity, and thus old reserves are probably widespread.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"195 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antisense CircRNA VvcircABH controls salt tolerance and the brassinosteroid signaling response by suppressing cognate mRNA splicing in grape","authors":"Zhen Gao, Yifan Su, Yaru Wang, Yeqi Li, Yue Wu, Xinru Sun, Yuxin Yao, Chao Ma, Jing Li, Yuanpeng Du","doi":"10.1111/nph.20306","DOIUrl":"https://doi.org/10.1111/nph.20306","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>Soil salinization is a major factor limiting the sustainable development of the grape industry. Circular RNAs (circRNAs) are more stable than linear mRNAs and are involved in stress responses. However, the biological functions and molecular mechanisms underlying antisense circRNAs in plants remain unclear.</li>\u0000<li>We identified the antisense circRNA <i>VvcircABH</i> through high-throughput sequencing. Using genetic transformation methods and molecular biological techniques, we analyzed the effects of <i>VvcircABH</i> on the response to salt stress and the mechanisms underlying its effects.</li>\u0000<li><i>VvcircABH</i> was located in the nucleus and upregulated by salt stress, while the expression level of its cognate gene <i>VvABH</i> (alpha/beta-hydrolase) was downregulated. <i>VvcircABH</i> overexpression or <i>VvABH</i> silencing greatly enhanced grape salt tolerance. <i>VvcircABH</i> could bind to the overlapping region and inhibits <i>VvABH</i> pre-mRNA splicing, thereby decreasing the expression level of <i>VvABH</i>. Additionally, <i>VvcircABH</i> repressed the additive effect of VvABH on the interaction between VvBRI1 (brassinosteroid-insensitive 1) and VvBKI1 (BRI1 kinase inhibitor 1), thus influencing the plant's response to BR, which plays important roles in plant salt tolerance.</li>\u0000<li>We conclude that <i>VvcircABH</i> and <i>VvABH</i> play distinct roles in the salt tolerance and brassinosteroid signaling response, and <i>VvcircABH</i> could govern the expression of <i>VvABH</i> by inhibiting its splicing.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"59 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}