BZR2-GRF5作为枢纽模块,通过油菜素类固醇-生长素串扰调控杂交甜菊的体外再生。

IF 8.1 1区 生物学 Q1 PLANT SCIENCES
New Phytologist Pub Date : 2025-06-20 DOI:10.1111/nph.70256
Ying Li, Wenhao Ma, Paiting Wang, Haiyao Ma, Hongxuan Li, Fen Bao, Yingming Fan, Shuaizheng Qi, Dingju Zhan, Zhenwu Pang, Jian Zhao, Jinfeng Zhang, Siyuan Chen
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引用次数: 0

摘要

木本植物的离体再生是种质资源增殖、保存和遗传改良的重要途径,主要通过体细胞胚胎发生(somatic embryogenesis, SE)和器官发生(organogenesis)两种途径实现。油菜素内酯(Brassinosteroids, BR)和生长素是调控植物再生的关键激素。然而,这些激素在杂交甜枫中的协同作用机制尚不清楚。我们证明了BR信号通路中的LsfBZR1/2与LsfGRF2/3/5相互作用。功能鉴定表明,过表达LsfBZR2或LsfGRF5均能显著促进愈伤组织增殖,抑制体胚形成,并提高器官发生中茎再生效率。发现LsfBZR2-LsfGRF5模块以组织特异性的方式差异调节生长素外排基因LsfWAG2。SE期间,LsfBZR2过表达抑制LsfWAG2表达,导致生长素积累,促进胚性愈伤组织(EC)增殖。相反,在器官发生途径中,LsfBZR2过表达会增强LsfWAG2的表达,从而降低生长素水平,从而加速茎部再生。本研究首次发现LsfBZR2-LsfGRF5模块在杂交甜枫的不同组织中具有双重功能。该模块通过BR和生长素之间的串扰,影响SE和器官发生。本研究为阐明杂交甜菊离体再生的分子机制提供了证据,对甜菊的选育和规模化生产具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
BZR2-GRF5 acts as a hub module in regulating in vitro regeneration through brassinosteroid–auxin crosstalk in hybrid sweetgum

  • In vitro plant regeneration is critical for germplasm multiplication, conservation, and genetic improvement in woody plants, primarily via two pathways: somatic embryogenesis (SE) and organogenesis. Brassinosteroids (BR) and auxin are key hormones regulating plant regeneration. However, the synergistic mechanism between these hormones in hybrid sweetgum remains poorly understood.
  • We demonstrated that LsfBZR1/2 in the BR signaling pathway interacts with LsfGRF2/3/5. Functional characterization revealed that overexpression of either LsfBZR2 or LsfGRF5 significantly promoted callus proliferation while suppressing somatic embryo formation during SE, and enhanced shoot regeneration efficiency in organogenesis.
  • LsfBZR2-LsfGRF5 module was found to differentially regulate the auxin efflux gene LsfWAG2 in a tissue-specific manner. During SE, LsfBZR2 overexpression suppresses LsfWAG2 expression, leading to auxin accumulation that promotes embryogenic callus (EC) proliferation. Conversely, in the organogenesis pathway, LsfBZR2 overexpression enhanced LsfWAG2 expression, which reduced auxin levels and consequently accelerated shoot regeneration.
  • This study represents the first identification of the LsfBZR2-LsfGRF5 module as a central regulator with dual functions in different tissues of hybrid sweetgum. Through the crosstalk between BR and auxin, this module influences both SE and organogenesis. Our study provides evidence supporting the elucidation of the molecular mechanism of in vitro regeneration in hybrid sweetgum, which is of great significance for breeding and large-scale production.
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来源期刊
New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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