{"title":"BZR2-GRF5作为枢纽模块,通过油菜素类固醇-生长素串扰调控杂交甜菊的体外再生。","authors":"Ying Li, Wenhao Ma, Paiting Wang, Haiyao Ma, Hongxuan Li, Fen Bao, Yingming Fan, Shuaizheng Qi, Dingju Zhan, Zhenwu Pang, Jian Zhao, Jinfeng Zhang, Siyuan Chen","doi":"10.1111/nph.70256","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>\n </p><ul>\n \n <li><i>In vitro</i> plant regeneration is critical for germplasm multiplication, conservation, and genetic improvement in woody plants, primarily via two pathways: somatic embryogenesis (SE) and organogenesis. Brassinosteroids (BR) and auxin are key hormones regulating plant regeneration. However, the synergistic mechanism between these hormones in hybrid sweetgum remains poorly understood.</li>\n \n <li>We demonstrated that LsfBZR1/2 in the BR signaling pathway interacts with LsfGRF2/3/5. Functional characterization revealed that overexpression of either <i>LsfBZR2</i> or <i>LsfGRF5</i> significantly promoted callus proliferation while suppressing somatic embryo formation during SE, and enhanced shoot regeneration efficiency in organogenesis.</li>\n \n <li>LsfBZR2-LsfGRF5 module was found to differentially regulate the auxin efflux gene <i>LsfWAG2</i> in a tissue-specific manner. During SE, <i>LsfBZR2</i> overexpression suppresses <i>LsfWAG2</i> expression, leading to auxin accumulation that promotes embryogenic callus (EC) proliferation. Conversely, in the organogenesis pathway, <i>LsfBZR2</i> overexpression enhanced <i>LsfWAG2</i> expression, which reduced auxin levels and consequently accelerated shoot regeneration.</li>\n \n <li>This study represents the first identification of the LsfBZR2-LsfGRF5 module as a central regulator with dual functions in different tissues of hybrid sweetgum. Through the crosstalk between BR and auxin, this module influences both SE and organogenesis. Our study provides evidence supporting the elucidation of the molecular mechanism of <i>in vitro</i> regeneration in hybrid sweetgum, which is of great significance for breeding and large-scale production.</li>\n </ul>\n </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"247 4","pages":"1694-1711"},"PeriodicalIF":8.1000,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"BZR2-GRF5 acts as a hub module in regulating in vitro regeneration through brassinosteroid–auxin crosstalk in hybrid sweetgum\",\"authors\":\"Ying Li, Wenhao Ma, Paiting Wang, Haiyao Ma, Hongxuan Li, Fen Bao, Yingming Fan, Shuaizheng Qi, Dingju Zhan, Zhenwu Pang, Jian Zhao, Jinfeng Zhang, Siyuan Chen\",\"doi\":\"10.1111/nph.70256\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>\\n </p><ul>\\n \\n <li><i>In vitro</i> plant regeneration is critical for germplasm multiplication, conservation, and genetic improvement in woody plants, primarily via two pathways: somatic embryogenesis (SE) and organogenesis. Brassinosteroids (BR) and auxin are key hormones regulating plant regeneration. However, the synergistic mechanism between these hormones in hybrid sweetgum remains poorly understood.</li>\\n \\n <li>We demonstrated that LsfBZR1/2 in the BR signaling pathway interacts with LsfGRF2/3/5. Functional characterization revealed that overexpression of either <i>LsfBZR2</i> or <i>LsfGRF5</i> significantly promoted callus proliferation while suppressing somatic embryo formation during SE, and enhanced shoot regeneration efficiency in organogenesis.</li>\\n \\n <li>LsfBZR2-LsfGRF5 module was found to differentially regulate the auxin efflux gene <i>LsfWAG2</i> in a tissue-specific manner. During SE, <i>LsfBZR2</i> overexpression suppresses <i>LsfWAG2</i> expression, leading to auxin accumulation that promotes embryogenic callus (EC) proliferation. Conversely, in the organogenesis pathway, <i>LsfBZR2</i> overexpression enhanced <i>LsfWAG2</i> expression, which reduced auxin levels and consequently accelerated shoot regeneration.</li>\\n \\n <li>This study represents the first identification of the LsfBZR2-LsfGRF5 module as a central regulator with dual functions in different tissues of hybrid sweetgum. Through the crosstalk between BR and auxin, this module influences both SE and organogenesis. Our study provides evidence supporting the elucidation of the molecular mechanism of <i>in vitro</i> regeneration in hybrid sweetgum, which is of great significance for breeding and large-scale production.</li>\\n </ul>\\n </div>\",\"PeriodicalId\":214,\"journal\":{\"name\":\"New Phytologist\",\"volume\":\"247 4\",\"pages\":\"1694-1711\"},\"PeriodicalIF\":8.1000,\"publicationDate\":\"2025-06-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New Phytologist\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/nph.70256\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/nph.70256","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
BZR2-GRF5 acts as a hub module in regulating in vitro regeneration through brassinosteroid–auxin crosstalk in hybrid sweetgum
In vitro plant regeneration is critical for germplasm multiplication, conservation, and genetic improvement in woody plants, primarily via two pathways: somatic embryogenesis (SE) and organogenesis. Brassinosteroids (BR) and auxin are key hormones regulating plant regeneration. However, the synergistic mechanism between these hormones in hybrid sweetgum remains poorly understood.
We demonstrated that LsfBZR1/2 in the BR signaling pathway interacts with LsfGRF2/3/5. Functional characterization revealed that overexpression of either LsfBZR2 or LsfGRF5 significantly promoted callus proliferation while suppressing somatic embryo formation during SE, and enhanced shoot regeneration efficiency in organogenesis.
LsfBZR2-LsfGRF5 module was found to differentially regulate the auxin efflux gene LsfWAG2 in a tissue-specific manner. During SE, LsfBZR2 overexpression suppresses LsfWAG2 expression, leading to auxin accumulation that promotes embryogenic callus (EC) proliferation. Conversely, in the organogenesis pathway, LsfBZR2 overexpression enhanced LsfWAG2 expression, which reduced auxin levels and consequently accelerated shoot regeneration.
This study represents the first identification of the LsfBZR2-LsfGRF5 module as a central regulator with dual functions in different tissues of hybrid sweetgum. Through the crosstalk between BR and auxin, this module influences both SE and organogenesis. Our study provides evidence supporting the elucidation of the molecular mechanism of in vitro regeneration in hybrid sweetgum, which is of great significance for breeding and large-scale production.
期刊介绍:
New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.