New Phytologist最新文献

{"title":"Exploring the metabolic daylength measurement system: implications for photoperiodic growth","authors":"Man-Wah Li,&nbsp;Joshua M. Gendron","doi":"10.1111/nph.20275","DOIUrl":"10.1111/nph.20275","url":null,"abstract":"<p>Photoperiod is an environmental signal that varies predictably across the year. Therefore, the duration of sunlight available for photosynthesis and in turn the ability of plants to accumulate carbon resources also fluctuates across the year. To adapt to these variations in photoperiod, the metabolic daylength measurement (MDLM) system measures the photosynthetic period rather than the absolute photoperiod, translating it into seasonal gene expression changes linked to photoperiodic growth. In this Tansley Insight, we briefly summarize the current understanding of the MDLM system and highlight gaps in our knowledge. Given the system's critical role in seasonal growth, understanding the MDLM system is essential for enhancing plant adaptation to different photoperiods and optimizing agricultural production.</p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"503-509"},"PeriodicalIF":8.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20275","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142630943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Centromeres are hotspots for chromosomal inversions and breeding traits in mango","authors":"Melanie J. Wilkinson,&nbsp;Kathleen McLay,&nbsp;David Kainer,&nbsp;Cassandra Elphinstone,&nbsp;Natalie L. Dillon,&nbsp;Matthew Webb,&nbsp;Upendra K. Wijesundara,&nbsp;Asjad Ali,&nbsp;Ian S. E. Bally,&nbsp;Norman Munyengwa,&nbsp;Agnelo Furtado,&nbsp;Robert J. Henry,&nbsp;Craig M. Hardner,&nbsp;Daniel Ortiz-Barrientos","doi":"10.1111/nph.20252","DOIUrl":"10.1111/nph.20252","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Chromosomal inversions can preserve combinations of favorable alleles by suppressing recombination. Simultaneously, they reduce the effectiveness of purifying selection enabling deleterious alleles to accumulate.</li>\u0000 \u0000 <li>This study explores how areas of low recombination, including centromeric regions and chromosomal inversions, contribute to the accumulation of deleterious and favorable loci in 225 <i>Mangifera indica</i> genomes from the Australian Mango Breeding Program.</li>\u0000 \u0000 <li>Here, we identify 17 chromosomal inversions that cover 7.7% (29.7 Mb) of the <i>M. indica</i> genome: eight pericentric (inversion includes the centromere) and nine paracentric (inversion is on one arm of the chromosome). Our results show that these large pericentric inversions are accumulating deleterious loci, while the paracentric inversions show deleterious levels above and below the genome wide average. We find that despite their deleterious load, chromosomal inversions contain small effect loci linked to variation in crucial breeding traits.</li>\u0000 \u0000 <li>These results indicate that chromosomal inversions have likely facilitated the evolution of key mango breeding traits. Our study has important implications for selective breeding of favorable combinations of alleles in regions of low recombination.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"899-913"},"PeriodicalIF":8.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant organic nitrogen nutrition: costs, benefits, and carbon use efficiency","authors":"Laura Tünnermann, Camila Aguetoni Cambui, Oskar Franklin, Patrizia Merkel, Torgny Näsholm, Regina Gratz","doi":"10.1111/nph.20285","DOIUrl":"https://doi.org/10.1111/nph.20285","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Differences in soil mobility and assimilation costs between organic and inorganic nitrogen (N) compounds would hypothetically induce plant phenotypic plasticity to optimize acquisition of, and performance on, the different N forms. Here we evaluated this hypothesis experimentally and theoretically.</jats:list-item> <jats:list-item>We grew Arabidopsis in split‐root setups combined with stable isotope labelling to study uptake and distribution of carbon (C) and N from <jats:sc>l</jats:sc>‐glutamine (<jats:sc>l</jats:sc>‐gln) and NO<jats:sub>3</jats:sub><jats:sup>−</jats:sup> and assessed the effect of the N source on biomass partitioning and carbon use efficiency (CUE).</jats:list-item> <jats:list-item>Analyses of stable isotopes showed that 40–48% of C acquired from <jats:sc>l</jats:sc>‐gln resided in plants, contributing 7–8% to total C of both shoots and roots. Plants grown on <jats:sc>l</jats:sc>‐gln exhibited increased root mass fraction and root hair length and a significantly lower N uptake rate per unit root biomass but displayed significantly enhanced CUE.</jats:list-item> <jats:list-item>Our data suggests that organic N nutrition is linked to a particular phenotype with extensive growth of roots and root hairs that optimizes for uptake of less mobile N forms. Increased CUE and lower N uptake per unit root growth may be key facets linked to the organic N phenotype.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"160 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small, but mitey: investigating the molecular genetic basis for mite domatia development and intraspecific variation in Vitis riparia using transcriptomics","authors":"Eleanore Jeanne Ritter,&nbsp;Carolyn D. K. Graham,&nbsp;Chad Niederhuth,&nbsp;Marjorie Gail Weber","doi":"10.1111/nph.20226","DOIUrl":"10.1111/nph.20226","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 1","pages":"215-231"},"PeriodicalIF":8.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20226","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards targeted engineering of promoters via deletion of repressive cis-regulatory elements","authors":"Flora Zhiqi Wang, Krishna K. Niyogi","doi":"10.1111/nph.20280","DOIUrl":"https://doi.org/10.1111/nph.20280","url":null,"abstract":"&lt;div&gt;Many plants with enhanced traits have been engineered via the overexpression of target genes conferring specific benefits to farmers and/or consumers. This is most commonly achieved via transgenic overexpression of either a native or a heterologous gene. However, very few of these transgenic crops have a practical impact due to negative public perceptions of conventional genetically modified (GM) crops and financially burdensome regulations. As an alternative approach, scientists have turned to CRISPR-mediated gene editing to generate transgene-free, gain-of-function alleles. In a recently published article in &lt;i&gt;New Phytologist&lt;/i&gt;, Wang &lt;i&gt;et al&lt;/i&gt;. (&lt;span&gt;2024&lt;/span&gt;; doi: 10.1111/nph.20141) demonstrate the potential to generate ideal gain-of-function plants via CRISPR/Cas9-based targeted deletion of repressive &lt;i&gt;cis&lt;/i&gt;-regulatory elements (CRE) from native promoters. This study not only provides a generalizable approach towards rationally generating gain-of-function promoter alleles with a solid mechanistic underpinning but also highlights the power of targeting repressive motifs conserved across species to achieve similar phenotypes (increased protein content) in both rice (a monocot) and soybean (a dicot). &lt;blockquote&gt;&lt;p&gt;‘… &lt;i&gt;this approach is informed by a solid mechanistic understanding of repressive CREs within promoters, presenting a more rational and systematic approach to achieving endogenous gene overexpression&lt;/i&gt;.’&lt;/p&gt;\u0000&lt;div&gt;&lt;/div&gt;\u0000&lt;/blockquote&gt;\u0000&lt;/div&gt;\u0000&lt;p&gt;There are several ways by which plant scientists have attempted to generate gain-of-function alleles using CRISPR (Fig. 1). The first and most widely adopted approach is unbiased, multiplex, CRISPR-mediated, mutagenesis of a target promoter with multiple guide RNAs (gRNAs) in hope of generating gain-of-function alleles via fortuitous mutagenesis of upstream regulatory sequences. Since the first demonstration of Cas9-driven promoter mutagenesis by Rodríguez-Leal &lt;i&gt;et al&lt;/i&gt;. (&lt;span&gt;2017&lt;/span&gt;), several groups have utilized this approach to upregulate their genes of interest (Song &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;; Zhou &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;; Karavolias &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;; Patel-Tupper &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;). However, this method tends to result in mostly loss-of-function (knock-out and knock-down) alleles, and some gain-of-function alleles isolated using this method may involve larger-scale rearrangements such as chromosomal inversions (Patel-Tupper &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;). Additionally, the underlying mechanism driving overexpression of the target gene is frequently not understood in this approach, limiting its potential for rational design and engineering of promoters. A second and more recently attempted approach involves the insertion of enhancers identified via genome enrichment or STARR-seq assays followed by subsequent introduction of the enhancer into the target promoter through CRISPR-mediated knock-in. This method is o","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"196 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mistletoes have higher hydraulic safety but lower efficiency in xylem traits than their hosts","authors":"Yun-Bing Zhang,&nbsp;Xian-Yan Huang,&nbsp;Marina Corrêa Scalon,&nbsp;Yan Ke,&nbsp;Jing-Xin Liu,&nbsp;Qin Wang,&nbsp;Wen-Hua Li,&nbsp;Da Yang,&nbsp;David S. Ellsworth,&nbsp;Yong-Jiang Zhang,&nbsp;Jiao-Lin Zhang","doi":"10.1111/nph.20257","DOIUrl":"10.1111/nph.20257","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Both mistletoes and their hosts are challenged by increasing drought, highlighting the necessity of understanding their comparative hydraulic properties. The high transpiration of mistletoes requires efficient water transport, while high xylem tensions demand strong embolism resistance, representing a hydraulic paradox.</li>\u0000 \u0000 <li>This study, conducted across four environments with different aridity indices in Yunnan, China, examined the xylem traits of 119 mistletoe–host species pairs.</li>\u0000 \u0000 <li>Mistletoes showed lower water use efficiency, indicating a more aggressive water use. They also showed lower hydraulic efficiency (lower vessel diameter and theoretical hydraulic conductivity) but higher safety (lower vulnerability index and higher conduit wall reinforcement, vessel grouping index, and wood density) compared with their hosts, supporting the trade-off between efficiency and safety. Environmental variation across sites significantly affected xylem trait comparisons between mistletoes and hosts. Additionally, the xylem traits of mistletoes were strongly influenced by host water supply efficiency. The overall xylem trait relationships in mistletoes and hosts were different.</li>\u0000 \u0000 <li>These findings stress the impact of host and site on the hydraulic traits of mistletoes, and suggest that mistletoes may achieve high transpiration by maintaining high stomatal conductance under low water potentials. This study illuminates the distinctive adaptation strategies of mistletoes due to their parasitic lifestyle.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"607-624"},"PeriodicalIF":8.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capital and income breeders among herbs: how relative biomass allocation into a storage organ relates to clonal traits, phenology and environmental gradients","authors":"Timothy Harris,&nbsp;Andrea Kučerová,&nbsp;Martin Bitomský,&nbsp;Alena Bartušková,&nbsp;Frederick Curtis Lubbe,&nbsp;Jitka Klimešová","doi":"10.1111/nph.20260","DOIUrl":"10.1111/nph.20260","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Perennial herbs of seasonal climates invest carbon into belowground storage organs (e.g. rhizomes) to support growth when photosynthetic acquisition cannot cover demands. An alternative explanation interprets storage allocation as surplus carbon that is undeployable for growth when plants are limited by nutrients/water. We analysed relative investments to rhizomes to see to which of these explanations they align, and asked whether they scale with biomass of aboveground organs in individual species and whether clonal growth traits, phenology or environmental conditions explain investment among populations or species.</li>\u0000 \u0000 <li>We measured biomass of rhizomes, aboveground stems and leaves in 20 temperate herbaceous perennial species, each at two localities, establishing allometric relationships for pairs of organs. We correlated relative rhizome investment with clonal traits, environmental gradients and phenology, across species.</li>\u0000 \u0000 <li>For pairs of organs, biomass typically scales isometrically. Interspecific allocation differences are largely explained by phenology. Neither interspecific nor intraspecific differences were explained by clonal traits or environment.</li>\u0000 \u0000 <li>Storage organs of perennial herbs do not comprise deposition of carbon surplus, but receive greater allocation in capital breeders (early-flowering), than among income breeders (late-flowering) relying on acquisition during growing season. Capital and income breeders in plants deserve further examination of benefits/costs.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 1","pages":"154-168"},"PeriodicalIF":8.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo detection of spectral reflectance changes associated with regulated heat dissipation mechanisms complements fluorescence quantum efficiency in early stress diagnosis","authors":"Sara Pescador-Dionisio,&nbsp;Maria Pilar Cendrero-Mateo,&nbsp;Adrián Moncholí-Estornell,&nbsp;Aida Robles-Fort,&nbsp;Miren I. Arzac,&nbsp;Begoña Renau-Morata,&nbsp;Beatriz Fernández-Marín,&nbsp;José Ignacio García-Plazaola,&nbsp;Rosa V. Molina,&nbsp;Carolina Rausell,&nbsp;José Moreno,&nbsp;Sergio G. Nebauer,&nbsp;Inmaculada García-Robles,&nbsp;Shari Van Wittenberghe","doi":"10.1111/nph.20253","DOIUrl":"10.1111/nph.20253","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"559-576"},"PeriodicalIF":8.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20253","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Turnip mosaic virus selectively subverts a PR-5 thaumatin-like, plasmodesmal protein to promote viral infection","authors":"Rongrong He,&nbsp;Yinzi Li,&nbsp;Mark A. Bernards,&nbsp;Aiming Wang","doi":"10.1111/nph.20233","DOIUrl":"10.1111/nph.20233","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 1","pages":"299-317"},"PeriodicalIF":8.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A consumer-oriented approach to define breeding targets for molecular breeding","authors":"Luis Felipe V. Ferrão,&nbsp;Camila F. Azevedo,&nbsp;Charles A. Sims,&nbsp;Patricio R. Munoz","doi":"10.1111/nph.20254","DOIUrl":"10.1111/nph.20254","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"711-721"},"PeriodicalIF":8.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20254","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}