New Phytologist最新文献

{"title":"Priming the pump: translational dynamics from seed to seedling transition under priming treatment","authors":"Naoto Sano","doi":"10.1111/nph.70156","DOIUrl":"https://doi.org/10.1111/nph.70156","url":null,"abstract":"<div>Seed germination is considered a developmental transition phase, as it is part of the process in which metabolically quiescent dry seeds grow into vigorous seedlings through metabolic reactivation after water uptake, accompanied by dramatic changes in the associated gene expression networks (Silva <i>et al</i>., <span>2016</span>). Seed priming is a pre-sowing technique used in crop production to improve germination performance, resulting in more vigorous and stress-tolerant seedlings (Pagano <i>et al</i>., <span>2023</span>). This technique stimulates the transition from seeds to seedlings by initiating controlled seed hydration and then drying before germination. The trade-off is that primed seeds generally age and lose viability more rapidly than unprimed seeds (Fabrissin <i>et al</i>., <span>2021</span>). An article recently published in <i>New Phytologist</i>, by Gran <i>et al</i>. (<span>2025</span>; doi: 10.1111/nph.70098) ‘Unravelling the dynamics of seed stored mRNAs during seed priming’, provides novel insights into the translational changes underlying the transition from seeds to seedlings through priming treatment. <blockquote><p><i>‘How can we capture dynamic changes in gene expression induced by seed priming? has been a major challenge in elucidating the molecular mechanisms underlying priming-related traits.’</i></p>\u0000<div></div>\u0000</blockquote>\u0000</div>\u0000<p>In seed plants, seeds function as dispersal units for the next generation, incorporating various survival strategies. The desiccation tolerance of seeds enables their survival in a quiescent state under extreme water-deficient conditions. Subsequently, under environmentally favourable conditions, seed germination and seedling establishment occur, accompanied by a complete loss of desiccation tolerance after germination (Sano & Verdier, <span>2024</span>). Another key environmental adaptation mechanism is seed dormancy, which prevents vivipary and, following seed dispersal, delays and staggers germination over time. The depth of dormancy responses to seasonal environmental changes determines the optimal timing of germination for maximizing seedling survival and growth (Sano & Marion-Poll, <span>2021</span>). Following dispersal from the mother plant, seeds are subjected to fluctuations in temperature and hydration–dehydration cycles, which alter the embryo's lifespan and could influence seed persistence in the soil seed bank under natural environmental conditions (Long <i>et al</i>., <span>2015</span>). A pre-sowing treatment, ‘seed priming’, mimics such environmental fluctuations. In agriculture, rapid and uniform germination is essential. Priming reduces dormancy and promotes germination through controlled hydration, followed by re-drying before desiccation tolerance is lost. This process enables storage and distribution of primed seeds before sowing. However, a phenomenon known as ‘overpriming’ causes seed death after re-drying, and primed seeds more generally have a r","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"36 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivating vibrant apples: the role of nitrogen signaling in orchestrating anthocyanin biosynthesis for enhanced fruit coloration and quality","authors":"Xiaofeng Zhou, Nan Ma","doi":"10.1111/nph.70158","DOIUrl":"https://doi.org/10.1111/nph.70158","url":null,"abstract":"<div>Plants respond to nitrogen fluctuations through sophisticated metabolic reprogramming, with anthocyanin accumulation serving as a hallmark of stress adaptation. Nitrogen signaling, as a core regulatory pathway in this process, finely regulates anthocyanin biosynthesis through an intricate network of transcription factors, metabolic pathways, and signaling cascades. However, the molecular mechanisms underlying this regulation remain elusive. In an article recently published in <i>New Phytologist</i>, Guo <i>et al</i>. (<span>2025</span>, doi: 10.1111/nph.70040) unveiled a small part of these mechanisms with their outstanding research work. They proposed an ‘ubiquitination–phosphorylation–hormone’ tripartite regulatory framework, revealing how nitrate signaling dynamically coordinates with gibberellin pathways through posttranslational modifications to precisely regulate anthocyanin biosynthesis. Their work systematically deciphers the molecular logic of nutrient–hormone cross talk, offering novel insights into the interplay between nitrate signaling and phytohormone interaction networks. This discovery is of great significance in terms of revealing the molecular mechanisms of plant adaptation to environmental changes, as well as for future applications in agriculture, ecology, and other fields. <blockquote><p><i>It unveils the dynamic integration of post-translational modifications… with hormonal cues, through spatiotemporally orchestrated multi-tiered interactions, thereby providing a conceptual framework for optimizing nitrogen–hormone balance in fruit crop cultivation systems</i>.</p>\u0000<div></div>\u0000</blockquote>\u0000</div>\u0000<p>Nitrogen, as a central element in plant life processes, not only provides the structural foundation for the synthesis of primary metabolites such as amino acids and nucleic acids but also functions as a metabolic hub by dynamically sensing environmental nitrogen availability. This regulatory process involves multilayered coordination mechanisms. Under nitrogen-deficient conditions, plants adopt a ‘survival-priority’ strategy for resource reallocation. For instance, <i>Arabidopsis</i> seedlings exhibit a 42% reduction in Chl content, specific accumulation of anthocyanins in leaves, and a marked increase in lateral root density (Scheible <i>et al</i>., <span>2004</span>; Peng <i>et al</i>., <span>2008</span>). Such adaptive remodeling is achieved through dual metabolic adjustments: Nitrate resupply rapidly induces the trehalose biosynthesis gene <i>AtTPS5</i> while suppressing trehalose-6-phosphate phosphatases (<i>AtTPPA/B</i>), forming a ‘metabolic valve’ that redirects carbon flux from starch synthesis toward phenylpropanoid pathways (Scheible <i>et al</i>., <span>2004</span>). Concurrently, downregulation of nitrogen-intensive enzymes such as phenylalanine ammonia-lyase enhances nitrogen recycling efficiency in <i>Nicotiana tabacum</i> xylem by 37% (Fritz <i>et al</i>., <span>2006</span>).</p>\u0000<p>At the transcriptional le","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"64 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mago nashi controls auxin-mediated embryo patterning in Arabidopsis by regulating transcript abundance","authors":"Liping Liu, Wen Gong, Regina Stöckl, Philipp Denninger, Uwe Schwartz, Mark A. Johnson, Thomas Dresselhaus","doi":"10.1111/nph.70154","DOIUrl":"https://doi.org/10.1111/nph.70154","url":null,"abstract":"<p>Establishing the apical-basal body axis is one of the earliest steps in embryo development of animals and plants. In the fruit fly <i>Drosophila</i>, for example, the axis is established by localized graded determinants in an initial syncytium (Bloom, <span>1996</span>; Moussian & Roth, <span>2005</span>). In vertebrates, axis formation is established through a sequence of interactions between neighboring cells and via cell movement (Czirok <i>et al</i>., <span>2004</span>; Mongera <i>et al</i>., <span>2019</span>). By contrast, plant embryogenesis has no syncytial phase, and each cell has a fixed position and does not move (Capron <i>et al</i>., <span>2009</span>). In the model plant <i>Arabidopsis thaliana</i> (Arabidopsis), body axis formation is already initiated with the asymmetric division of the zygote. Zygotic division gives rise to a smaller apical daughter cell from which most of the embryo will develop and a large basal daughter cell, which will form the suspensor connecting the embryo to the maternal tissue. Two principal pathways regulate the establishment of the apical-basal axis in Arabidopsis: One involves activation of the transcription factors <i>WUSCHEL-RELATED HOMEOBOX 2</i> (<i>WOX2</i>) and <i>WOX8</i>, and the other one involves PIN-FORMED (PIN)-mediated auxin transport and temporal activity of the auxin response machinery (Lau <i>et al</i>., <span>2012</span>; Robert <i>et al</i>., <span>2013</span>; Palovaara <i>et al</i>., <span>2016</span>; Dresselhaus & Jürgens, <span>2021</span>). <i>WOX2</i> and <i>WOX8</i> are initially co-expressed in the zygote and are thereafter restricted to the apical and basal daughter cells, marking the apical and basal cell lineages, respectively (Haecker <i>et al</i>., <span>2004</span>; Breuninger <i>et al</i>., <span>2008</span>). Plants utilize directional transport of auxin to generate an asymmetric auxin response that specifies the embryonic apical-basal axis (Friml <i>et al</i>., <span>2003</span>; Weijers <i>et al</i>., <span>2006</span>; Ueda <i>et al</i>., <span>2011</span>). Suspensor-expressed auxin efflux carrier PIN7 mediates polar auxin flow from the suspensor toward the embryo proper, which is required for embryo development (Friml <i>et al</i>., <span>2003</span>; Robert <i>et al</i>., <span>2013</span>). Later during embryogenesis, the onset of localized auxin biosynthesis mediates polarization of the auxin efflux carriers PIN1, which is required for the specification of basal embryonic structures (e.g. the root pole) (Ikeda <i>et al</i>., <span>2009</span>; Eklund <i>et al</i>., <span>2010</span>; Robert <i>et al</i>., <span>2013</span>).</p>\u0000<p>Mago nashi (Mago), which was originally identified in <i>Drosophila</i>, is required for polarity establishment during early embryogenesis. Mago is a maternal component of an mRNP complex that is required for polarized localization of <i>oskar</i> mRNA to the posterior pole for axis formation in <i>Drosophila</i> oocytes","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"68 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AMF diversity promotes plant community phosphorus acquisition and reduces carbon costs per unit of phosphorus","authors":"Sören Eliot Weber, Jordi Bascompte, Ansgar Kahmen, Pascal A. Niklaus","doi":"10.1111/nph.70161","DOIUrl":"https://doi.org/10.1111/nph.70161","url":null,"abstract":"<p>\u0000</p><ul>\u0000<li>Plants may benefit from more diverse communities of arbuscular mycorrhizal fungi (AMF), as functional complementarity of AMF may allow for increased resource acquisition, and because a high AMF diversity increases the probability of plants matching with an optimal AMF symbiont.</li>\u0000<li>We repeatedly radiolabeled plants and AMF in the glasshouse over <i>c.</i> 9 months to test how AMF species richness (SR) influences the exchange of plant C (¹⁴C) for AMF P (³²P &amp; ³³P) and resulting shoot nutrients and mass from a biodiversity–ecosystem functioning perspective.</li>\u0000<li>Plant P acquisition via AMF increased with sown AMF SR, as did shoot biomass, shoot P, and shoot N. The rate of plant C transferred to AMF for this P (C:P) decreased with sown AMF SR.</li>\u0000<li>Plants in plant communities benefit from inoculation with a variety of AMF species via more favorable resource exchange. Surprisingly, this effect did not differ among functionally distinct communities comprised entirely of either legumes, nonlegume forbs, or C3 grasses.</li>\u0000</ul><p></p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"35 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRUSTing transposable elements to create ultra-clean transgene insertions","authors":"Ryan Swanson, Brooke Diehl, R. Keith Slotkin","doi":"10.1111/nph.70157","DOIUrl":"https://doi.org/10.1111/nph.70157","url":null,"abstract":"&lt;div&gt;The molecular improvement of crops necessitates the placement of custom DNA sequences, known as ‘genes of interest’ (GOI), into their genomes. A GOI can be foreign DNA (transgenic) or sequences copied from that same crop genome (cisgenic). The process of adding the GOI into the new genome, called transformation, is inefficient and relies on the use of selectable marker genes, so the majority of plant material that did not successfully undergo transformation is culled. However, after transformation and selection, these selectable marker genes become a liability. They present a significant hurdle in the regulatory process because they introduce foreign (transgenic) materials and have the potential to spread herbicide or antibiotic resistance to wild plant relatives. Marker-free DNA insertion is important to the field of plant biotechnology because it minimizes the potential impact to the environment, reduces the regulatory burden, and improves consumer acceptance (Singh &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;). At the same time, because transformation occurs at a low success rate and requires selection, efficient marker-free transgenesis is currently not feasible (Kausch &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2021&lt;/span&gt;). In practice, today's crop improvement transgenes possess both a resistance gene and a GOI (Fig. 1a), for example, both an antibiotic resistance gene and a stress tolerance trait. Methods have been developed to remove the selectable marker after transgenesis with the goal of leaving only the remaining GOI (Nishizawa-Yokoi &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2015&lt;/span&gt;). For example, Cre-&lt;i&gt;lox&lt;/i&gt; is a recombination system used to remove the selectable marker from the transgene after transgenesis and selection, leaving a small 34 bp &lt;i&gt;LoxP&lt;/i&gt; footprint adjacent to the GOI (Fig. 1b; Yarmolinsky &amp; Hoess, &lt;span&gt;2015&lt;/span&gt;). Cre-&lt;i&gt;lox&lt;/i&gt; has been used extensively for commercial crop production; however, the system has significant challenges, such as inefficient excision of the marker gene and the lack of total elimination of foreign DNA. &lt;blockquote&gt;&lt;p&gt;&lt;i&gt;‘For plants or lines that are recalcitrant to transformation, this reduces the number of transformed individuals required to generate the user's desired final GOI event.’&lt;/i&gt;&lt;/p&gt;\u0000&lt;div&gt;&lt;/div&gt;\u0000&lt;/blockquote&gt;\u0000&lt;/div&gt;\u0000&lt;figure&gt;&lt;picture&gt;\u0000&lt;source media=\"(min-width: 1650px)\" srcset=\"/cms/asset/dbba6a48-67b7-4c35-a766-9bac85dff400/nph70157-fig-0001-m.jpg\"/&gt;&lt;img alt=\"Details are in the caption following the image\" data-lg-src=\"/cms/asset/dbba6a48-67b7-4c35-a766-9bac85dff400/nph70157-fig-0001-m.jpg\" loading=\"lazy\" src=\"/cms/asset/5bf852cf-0494-46c9-ab2e-03a76f5a4ff2/nph70157-fig-0001-m.png\" title=\"Details are in the caption following the image\"/&gt;&lt;/picture&gt;&lt;figcaption&gt;\u0000&lt;div&gt;&lt;strong&gt;Fig. 1&lt;span style=\"font-weight:normal\"&gt;&lt;/span&gt;&lt;/strong&gt;&lt;div&gt;Open in figure viewer&lt;i aria-hidden=\"true\"&gt;&lt;/i&gt;&lt;span&gt;PowerPoint&lt;/span&gt;&lt;/div&gt;\u0000&lt;/div&gt;\u0000&lt;div&gt;Transgenesis and transposition combined in transposon-mediated ultra-clean selectable transform","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"23 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carposphere microbiota alters grape volatiles and shapes the wine grape typicality","authors":"Menglong Liu,&nbsp;Xuenan Yao,&nbsp;Haiqi Wang,&nbsp;Xiaobo Xu,&nbsp;Junhua Kong,&nbsp;Yongjian Wang,&nbsp;Weiping Chen,&nbsp;Huiqing Bai,&nbsp;Zixuan Wang,&nbsp;Mathabatha Evodia Setati,&nbsp;Sam Crauwels,&nbsp;Erna Blancquaert,&nbsp;Peige Fan,&nbsp;Zhenchang Liang,&nbsp;Zhanwu Dai","doi":"10.1111/nph.70152","DOIUrl":"10.1111/nph.70152","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>While specific environments are known to shape plant metabolomes and the makeup of their associated microbiome, it is as yet unclear whether carposphere microbiota contribute to the characteristics of grape fruit flavor of a particular wine region.</li>\u0000 \u0000 <li>Here, carposphere microbiomes and berry transcriptomes and metabolomes of three grape cultivars growing at six geographic sites were analyzed.</li>\u0000 \u0000 <li>The composition of the carposphere microbiome was determined mainly by environmental conditions, rather than grape genotype. Bacterial microbiota likely contributed to grape volatile profiles. Particularly, candidate operational taxonomic units (OTUs) in genus Sphingomonas were highly correlated with grape C6 aldehyde volatiles (also called green leaf volatiles, GLVs), which contribute to a fresh taste. Furthermore, a core set of expressed genes was enriched in lipid metabolism, which is responsible for bacterial colonization and C6 aldehyde volatile synthesis activation. Finally, a similar grape volatile profile was observed after inoculating the berry skin of two grape cultivars with <i>Sphingomonas</i> sp., thus providing evidence for the hypothetical microbe–metabolite relationship.</li>\u0000 \u0000 <li>These results provide novel insight into how the environment–microbiome–plant quality (E × Mi × Q) interaction may shape berry flavor and thereby typicality, serving as a foundation for decision-making in vineyard microbial management.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"246 5","pages":"2280-2294"},"PeriodicalIF":8.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Holo-omics disentangle drought response and biotic interactions among plant, endophyte and pathogen","authors":"Peilin Chen,&nbsp;Qingyi Yu,&nbsp;Cong Wang,&nbsp;Liliam Montoya,&nbsp;Patrick T. West,&nbsp;Ling Xu,&nbsp;Nelle Varoquaux,&nbsp;Benjamin Cole,&nbsp;Kim K. Hixson,&nbsp;Young-Mo Kim,&nbsp;Ling Liu,&nbsp;Baodan Zhang,&nbsp;Jie Zhang,&nbsp;Baiyang Li,&nbsp;Elizabeth Purdom,&nbsp;John Vogel,&nbsp;Christer Jansson,&nbsp;Robert B. Hutmacher,&nbsp;Jeffery A. Dahlberg,&nbsp;Devin Coleman-Derr,&nbsp;Peggy G. Lemaux,&nbsp;John W. Taylor,&nbsp;Cheng Gao","doi":"10.1111/nph.70155","DOIUrl":"10.1111/nph.70155","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Holo-omics provide a novel opportunity to study the interactions among fungi from different functional guilds in host plants in field conditions.</li>\u0000 \u0000 \u0000 <li>We address the entangled responses of plant pathogenic and endophytic fungi associated with sorghum when droughted through the assembly of the most abundant fungal, endophyte genome from rhizospheric metagenomic sequences followed by a comparison of its metatranscriptome with the host plant metabolome and transcriptome.</li>\u0000 \u0000 \u0000 <li>The rise in relative abundance of endophytic <i>Acremonium persicinum</i> (operational taxonomic unit 5 (OTU5)) in drought co-occurs with a rise in fungal membrane dynamics and plant metabolites, led by ethanolamine, a key phospholipid membrane component. The negative association between endophytic <i>A. persicinum</i> (OTU5) and plant pathogenic fungi co-occurs with a rise in expression of the endophyte's biosynthetic gene clusters coding for secondary compounds. Endophytic <i>A. persicinum</i> (OTU5) and plant pathogenic fungi are negatively associated under preflowering drought but not under postflowering drought, likely a consequence of variation in fungal fitness responses to changes in the availability of water and niche space caused by plant maturation over the growing season.</li>\u0000 \u0000 \u0000 <li>Our findings suggest that the dynamic biotic interactions among host, beneficial and harmful microbiota in a changing environment can be disentangled by a blending of field observation, laboratory validation, holo-omics and ecological modelling.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"246 6","pages":"2702-2717"},"PeriodicalIF":8.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ring-specific vulnerability to embolism reveals accumulation of damage in the xylem","authors":"Jaycie C. Fickle,&nbsp;German Vargas G.,&nbsp;William R. L. Anderegg","doi":"10.1111/nph.70137","DOIUrl":"10.1111/nph.70137","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"246 5","pages":"2046-2058"},"PeriodicalIF":8.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A FOX transcription factor phosphorylated for regulation of autophagy facilitates fruiting body development in Sclerotinia sclerotiorum","authors":"Genglin Zhu,&nbsp;Qi Zuo,&nbsp;Sirui Liu,&nbsp;Peiyi Zheng,&nbsp;Yanhua Zhang,&nbsp;Xianghui Zhang,&nbsp;Jeffrey A. Rollins,&nbsp;Jinliang Liu,&nbsp;Hongyu Pan","doi":"10.1111/nph.70151","DOIUrl":"10.1111/nph.70151","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Autophagy is a recycling process by which eukaryotic cells degrade their own components, and the fruiting body (sexual structure) is a necessary structure for some plant pathogenic fungi to start the infection cycle. However, the transcriptional regulation of plant pathogenic fungal autophagy and autophagy regulating sexual reproduction remains elusive.</li>\u0000 \u0000 \u0000 <li>Here, we provide the report linking autophagy transcription and fruiting body development in phytopathogenic fungi. The forkhead box transcription factor (FOX TF) SsFoxE2 in <i>Sclerotinia sclerotiorum</i> (Ss) binds to the promoters of <i>ATG</i> genes, thus promoting their transcription. SsFoxE2 is phosphorylated by AMP-activated protein kinase (AMPK) SsSnf1, and the phosphorylated SsFoxE2 interacts with (translationally controlled tumor protein) SsTctp1, leading to enhanced stability and <i>ATG</i> transcription activity of SsFoxE2.</li>\u0000 \u0000 \u0000 <li>Importantly, the regulation of autophagy by SsFoxE2 affects the balance of the ubiquitination system and the early development of the fruiting body, which directly determines the occurrence and prevalence of plant disease. Furthermore, transcriptional binding of FOX TF to <i>ATG</i> gene promoters is conserved in phytopathogenic fungi.</li>\u0000 \u0000 \u0000 <li>Taken together, our results bring new insights into pathogen initiation in phytopathogenic fungi and connect it to other autophagy-regulated processes in plant pathogens.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"246 6","pages":"2683-2701"},"PeriodicalIF":8.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and environmental drivers of intraspecific variation in foliar metabolites in a tropical tree community","authors":"Yunyun He,&nbsp;Robert R. Junker,&nbsp;Jianhua Xiao,&nbsp;Jesse R. Lasky,&nbsp;Min Cao,&nbsp;Mengesha Asefa,&nbsp;Nathan G. Swenson,&nbsp;Guorui Xu,&nbsp;Jie Yang,&nbsp;Brain E. Sedio","doi":"10.1111/nph.70146","DOIUrl":"10.1111/nph.70146","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 </p><ul>\u0000 \u0000 \u0000 <li>Plant interactions with abiotic and biotic environments are mediated by diverse metabolites, which are crucial for stress response and defense. These metabolites can not only support diversity by shaping species niche differences but also display heritable and plastic intraspecific variation, which few studies have quantified in terms of their relative contributions.</li>\u0000 \u0000 \u0000 <li>To address this shortcoming, we used untargeted metabolomics to annotate and quantify foliar metabolites and restriction-site associated DNA (RAD) sequencing to assess genetic distances among 300 individuals of 10 locally abundant species from a diverse tropical community in Southwest China. We quantified the relative contributions of relatedness and the abiotic and biotic environment to intraspecific metabolite variation, considering different biosynthetic pathways.</li>\u0000 \u0000 \u0000 <li>Intraspecific variation contributed most to community-level metabolite diversity, followed by species-level variation. Biotic factors had the largest effect on total and secondary metabolites, while abiotic factors strongly influenced primary metabolites, particularly carbohydrates. The relative importance of these factors varied widely across different biosynthetic pathways and different species.</li>\u0000 \u0000 \u0000 <li>Our findings highlight that intraspecific variation is an essential component of community-level metabolite diversity. Furthermore, species rely on distinct classes of metabolites to adapt to environmental pressures, with genetic, abiotic, and biotic factors playing pathway-specific roles in driving intraspecific variation.</li>\u0000 </ul>\u0000 \u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"246 6","pages":"2551-2564"},"PeriodicalIF":8.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}