New Phytologist最新文献

{"title":"The rhizobial type III effectors ErnA and Sup3 hijack the SUMOylation pathway to trigger nodule formation in Aeschynomene species","authors":"Fazal Haq,&nbsp;Alicia Camuel,&nbsp;Mélanie Carcagno,&nbsp;Emanuele G. Biondi,&nbsp;Valérie Pacquit,&nbsp;Laurent Deslandes,&nbsp;Eric Giraud,&nbsp;Peter Mergaert","doi":"10.1111/nph.70334","DOIUrl":"10.1111/nph.70334","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"247 4","pages":"1826-1836"},"PeriodicalIF":8.3,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70334","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity factor p22 of tomato chlorosis virus interferes with abscisic acid-mediated antiviral defenses by disrupting the function of WRKY81 in tomato","authors":"Lianyi Zang,&nbsp;Pei Wang,&nbsp;Qin Yan,&nbsp;Sijia Liu,&nbsp;Mengxue Xiao,&nbsp;Dezhi Peng,&nbsp;Jinhao Xie,&nbsp;Liping Wang,&nbsp;Weihong Lin,&nbsp;Laihua Dong,&nbsp;Simon C. Groen,&nbsp;Xiaoping Zhu,&nbsp;Tao Zhou","doi":"10.1111/nph.70267","DOIUrl":"10.1111/nph.70267","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Abscisic acid (ABA) is essential for plant resistance to both biotic and abiotic stresses, but its regulation during virus infection and role in antiviral defense remain poorly understood. Here, we report that overexpression of SlWRKY81 led to increased ABA accumulation, whereas silencing <i>SlWRKY81</i> reduced ABA levels in tomato plants. Further analysis revealed that SlWRKY81 directly binds the promoters of ABA biosynthesis genes <i>SlABA2</i> and <i>SlABA3</i>, thereby activating their expression.</li>\u0000 \u0000 <li>Infection by tomato chlorosis virus (ToCV) inhibited ABA content significantly. Consistently, heterologous expression of ToCV-encoded p22 pathogenicity protein in tomato resulted in an ABA-deficient phenotype. Additionally, exogenous ABA application significantly improved antiviral defense against ToCV, whereas ABA-deficient tomato mutant <i>sitiens</i> exhibited higher susceptibility to ToCV.</li>\u0000 \u0000 <li>While it has been demonstrated that ABA modulates virus accumulation via fine-tuning immunity, viral counter-mechanisms remain unclear. We demonstrate that p22 inhibited the nuclear localization of SlWRKY81. Furthermore, p22 entered the nucleus and impaired the DNA binding activity of SlWRKY81, simultaneously accelerating its degradation through the ubiquitin-26S proteasome pathway via an unknown mechanism.</li>\u0000 \u0000 <li>Our findings uncover the positive role of SlWRKY81 in regulating ABA biosynthesis in tomato and reveal mechanisms by which a viral pathogenicity factor interferes with ABA-mediated antiviral defenses.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"247 4","pages":"1837-1854"},"PeriodicalIF":8.3,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144341265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic evidence unveils the genetic architecture and evolution of the S-locus controlling heterostyly in Rubiaceae","authors":"Shuai Yuan,&nbsp;Spencer C. H. Barrett,&nbsp;Chuqiao Tang,&nbsp;Yangmeng Zhang,&nbsp;Qilin Sun,&nbsp;Zhongtao Zhao,&nbsp;Yu Zhang,&nbsp;Dianxiang Zhang,&nbsp;Shixiao Luo","doi":"10.1111/nph.70311","DOIUrl":"10.1111/nph.70311","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>The <i>S</i>-locus supergene controlling heterostyly has multiple angiosperm origins and is characterized by convergent evolution in form and function. The genetic architecture of floral polymorphism has been studied in several unrelated families, but not Rubiaceae, which has the largest number of heterostylous species.</li>\u0000 \u0000 <li>We assembled genomes of the long- and short-styled morphs of distylous <i>Mussaenda lancipetala</i> and investigated the structure and evolution of the <i>S-locus</i> in three <i>Mussaenda</i> species to evaluate evidence for convergence in genetic architecture and gene function in unrelated families.</li>\u0000 \u0000 <li>Genome assemblies and population genomics indicated that the <i>S</i>-locus in <i>M. lancipetala</i> is comprised of three hemizygous genes – <i>MuIAA</i>, <i>MuGA3ox</i>, and <i>MuAPs</i> – present only in the S-morph. In each species, <i>MuIAA</i>, involved in the auxin response pathway, was highly expressed in pistils and floral tubes of the S-morph, and is a likely candidate gene controlling style length. Molecular evolution analysis indicated that the <i>S</i>-haplotype has accumulated repetitive sequences, <i>S</i>-linked genes showed no evidence of relaxed purifying selection, and the evolutionary assembly of the <i>S</i>-locus involved stepwise duplication.</li>\u0000 \u0000 <li>Our findings provide support for the prevalence of hemizygosity in <i>S</i>-locus genes and have identified molecular pathways underpinning convergent morphological evolution of clear adaptive significance.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"247 4","pages":"1925-1941"},"PeriodicalIF":8.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BZR2-GRF5 acts as a hub module in regulating in vitro regeneration through brassinosteroid–auxin crosstalk in hybrid sweetgum","authors":"Ying Li,&nbsp;Wenhao Ma,&nbsp;Paiting Wang,&nbsp;Haiyao Ma,&nbsp;Hongxuan Li,&nbsp;Fen Bao,&nbsp;Yingming Fan,&nbsp;Shuaizheng Qi,&nbsp;Dingju Zhan,&nbsp;Zhenwu Pang,&nbsp;Jian Zhao,&nbsp;Jinfeng Zhang,&nbsp;Siyuan Chen","doi":"10.1111/nph.70256","DOIUrl":"10.1111/nph.70256","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li><i>In vitro</i> plant regeneration is critical for germplasm multiplication, conservation, and genetic improvement in woody plants, primarily via two pathways: somatic embryogenesis (SE) and organogenesis. Brassinosteroids (BR) and auxin are key hormones regulating plant regeneration. However, the synergistic mechanism between these hormones in hybrid sweetgum remains poorly understood.</li>\u0000 \u0000 <li>We demonstrated that LsfBZR1/2 in the BR signaling pathway interacts with LsfGRF2/3/5. Functional characterization revealed that overexpression of either <i>LsfBZR2</i> or <i>LsfGRF5</i> significantly promoted callus proliferation while suppressing somatic embryo formation during SE, and enhanced shoot regeneration efficiency in organogenesis.</li>\u0000 \u0000 <li>LsfBZR2-LsfGRF5 module was found to differentially regulate the auxin efflux gene <i>LsfWAG2</i> in a tissue-specific manner. During SE, <i>LsfBZR2</i> overexpression suppresses <i>LsfWAG2</i> expression, leading to auxin accumulation that promotes embryogenic callus (EC) proliferation. Conversely, in the organogenesis pathway, <i>LsfBZR2</i> overexpression enhanced <i>LsfWAG2</i> expression, which reduced auxin levels and consequently accelerated shoot regeneration.</li>\u0000 \u0000 <li>This study represents the first identification of the LsfBZR2-LsfGRF5 module as a central regulator with dual functions in different tissues of hybrid sweetgum. Through the crosstalk between BR and auxin, this module influences both SE and organogenesis. Our study provides evidence supporting the elucidation of the molecular mechanism of <i>in vitro</i> regeneration in hybrid sweetgum, which is of great significance for breeding and large-scale production.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"247 4","pages":"1694-1711"},"PeriodicalIF":8.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms driving functional divergence of transcription factor paralogs","authors":"Isabella J. Higgins, Sarah G. Choudury, Aman Y. Husbands","doi":"10.1111/nph.70309","DOIUrl":"https://doi.org/10.1111/nph.70309","url":null,"abstract":"SummaryTranscription factors (TFs) are core components of the regulatory toolkits that control gene expression. The sophistication of these regulatory toolkits dramatically increased during Eukaryotic evolution, accomplished in part by the duplication of existing TFs and the subsequent repurposing of these new paralogs. This process, termed functional divergence, drove the evolution of increasingly elaborate programs of gene expression and, in turn, cellular and organismal complexity. Mechanisms generating functional divergence of TF paralogs are thus of significant interest. Here, we review the numerous mechanisms that can lead to divergence of TF paralogs, drawing on studies from across Eukaryota but with a special emphasis on the plant kingdom. We end by placing these mechanisms back into a broader evolutionary context.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"12 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional evidence of pluripotency during development of the leaf gall formed by grape phylloxera (Daktulosphaira vitifoliae)","authors":"Jack C. Schultz,&nbsp;Mélanie J. A. Body,&nbsp;Patrick P. Edger,&nbsp;Sarah Melissa Witiak,&nbsp;Heidi M. Appel","doi":"10.1111/nph.70241","DOIUrl":"10.1111/nph.70241","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"247 4","pages":"1712-1726"},"PeriodicalIF":8.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70241","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards the control of biofilm formation in Anabaena (Nostoc) sp. PCC7120: novel insights into the genes involved and their regulation","authors":"Irene Olivan-Muro,&nbsp;Jorge Guío,&nbsp;Germán Alonso-Tolo,&nbsp;Emma Sevilla,&nbsp;María F. Fillat","doi":"10.1111/nph.70317","DOIUrl":"10.1111/nph.70317","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"247 4","pages":"1805-1825"},"PeriodicalIF":8.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70317","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The collector practices that shape spatial, temporal, and taxonomic bias in herbaria.","authors":"Ryan J Schmidt,Kristen E Saban,Lena Struwe,Charles C Davis","doi":"10.1111/nph.70297","DOIUrl":"https://doi.org/10.1111/nph.70297","url":null,"abstract":"Natural history collections (NHCs) are essential for studying biodiversity. Although spatial, temporal, and taxonomic biases in NHCs affect analyses, the influence of collector practices on biases remains largely unexplored. We utilized one million digitized specimens collected in the northeastern United States by c. 10 000 collectors to investigate how collector practices shape spatial, temporal, and taxonomic biases in NHCs; and similarities and differences between practices of more- and less-prolific collectors. We identified six common collector practices, or collection norms: collectors generally collected different species, from multiple locations, from sites sampled by others, during the principal growing season, species identifiable outside peak collecting months, and species from species-poor families and genera. Some norms changed over decades, with different taxa favored during different periods. Collection norms have increased taxonomic coverage in NHCs; however, collectors typically avoided large, taxonomically complex groups, causing their underrepresentation in NHCs. Less-prolific collectors greatly enhanced coverage by collecting during more months and from less-sampled locations. We assert that overall collection biases are shaped by shared predictable collection norms rather than random practices of individual collectors. Predictable biases offer an opportunity to more effectively address biases in future biodiversity models.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"98 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COmapper: high-resolution mapping of meiotic crossovers by long-read sequencing in Arabidopsis","authors":"Dohwan Byun,&nbsp;Namil Son,&nbsp;Heejin Kim,&nbsp;Jaeil Kim,&nbsp;Jihye Park,&nbsp;Sang-jun Park,&nbsp;Hyein Kim,&nbsp;Jaebeom Kim,&nbsp;Juhyun Kim,&nbsp;Seula Lee,&nbsp;Youbong Hyun,&nbsp;Piotr A. Ziolkowski,&nbsp;Ian R. Henderson,&nbsp;Kyuha Choi","doi":"10.1111/nph.70304","DOIUrl":"10.1111/nph.70304","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"247 4","pages":"1942-1957"},"PeriodicalIF":8.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70304","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144319845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The increasingly powerful term mycorrhiza warrants attention","authors":"Paola Bonfante,&nbsp;Andrea Genre","doi":"10.1111/nph.70324","DOIUrl":"10.1111/nph.70324","url":null,"abstract":"&lt;p&gt;As climate pressures intensify, human pressure on the environment increases, and the search for sustainable agricultural solutions becomes more urgent, interest in plant symbioses has surged across disciplines, policy makers, and consumers. Among the many plant–microbial symbioses, arbuscular mycorrhizal (AM) association represents one of the most ancient and ecologically significant relationships between plants and fungi, with major implications for plant nutrition, soil health, and ecosystem resilience (Martin &amp; van der Heijden, &lt;span&gt;2024&lt;/span&gt;). Indeed, AM fungi colonize the roots of &lt;i&gt;c&lt;/i&gt;. 72% of terrestrial plants and are known to support uptake of phosphorus and other minerals, reduce plant stress, and improve soil structure (van der Heijden &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2015&lt;/span&gt;; Genre &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2020&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;At the same time, advances in molecular biology and genetics are shedding new light on the regulatory circuits underpinning these interactions. In a recent landmark study published in &lt;i&gt;Nature&lt;/i&gt;, Cook &lt;i&gt;et al&lt;/i&gt;. (&lt;span&gt;2025&lt;/span&gt;) demonstrate that a single point mutation in a gene encoding a nuclear-localized cyclic nucleotide-gated channel (CNGC15) can induce spontaneous calcium oscillations, enhancing the establishment of both nitrogen-fixing and mycorrhizal symbioses, even under nutrient-rich conditions that typically suppress them. This discovery represents a leap forward in our ability to manipulate plant–microbe interactions and paves the way for integrating endosymbioses more robustly into future cropping systems.&lt;/p&gt;&lt;p&gt;This momentum in scientific progress and broad public interest also highlights a critical conceptual issue: the increasingly frequent misuse of the term &lt;i&gt;mycorrhiza&lt;/i&gt;, which threatens to undermine clear scientific communication at a time when interdisciplinary understanding is more important than ever.&lt;/p&gt;&lt;p&gt;The term mycorrhiza was introduced by Albert Bernhard Frank in 1885 to describe the mutualistic association between plant roots and fungi. Derived from the Greek &lt;i&gt;mykes&lt;/i&gt; (fungus) and &lt;i&gt;rhiza&lt;/i&gt; (root), it refers specifically to the symbiotic relationship, not to the fungus itself. Yet, a troubling trend has emerged, in which the term is increasingly used to denote the fungal partner – particularly in statements, such as ‘plants interact with arbuscular mycorrhiza’, a formulation that depicts mycorrhiza as the symbiont rather than the symbiosis.&lt;/p&gt;&lt;p&gt;While seemingly minor, this misusage signals a broader conceptual slippage. Such phrasing risks reinforcing a plant-centric bias that oversimplifies the intricate, bidirectional nature of the symbiosis. By reducing the fungal partner to an anonymous, passive player or, worse, conflating it with the entire symbiotic phenomenon, we miss the opportunity to appreciate the specificity and agency of these microorganisms. Analogously, one would not say that legumes interact with ‘nitrogen-fixing nodules’ but with rhizobia – the ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"247 4","pages":"1555-1556"},"PeriodicalIF":8.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70324","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}