New Phytologist最新文献

{"title":"DynG: a dynamic scaling factor for thermographic stomatal conductance estimation under changing environmental conditions","authors":"Jiayu Zhang, Elias Kaiser, Leo F. M. Marcelis, Silvere Vialet‐Chabrand","doi":"10.1111/nph.70555","DOIUrl":"https://doi.org/10.1111/nph.70555","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Thermal imaging is a key plant phenotyping and monitoring technique but faces major bottlenecks in accurately and efficiently inferring stomatal conductance (<jats:italic>g</jats:italic><jats:sub>sw</jats:sub>) from leaf temperature. The conductance index (<jats:italic>I</jats:italic><jats:sub>g</jats:sub>) was previously proposed to estimate <jats:italic>g</jats:italic><jats:sub>sw</jats:sub> from thermography by linking temperature differences between real and artificial leaves (ALs) based on the leaf energy balance. However, <jats:italic>I</jats:italic><jats:sub>g</jats:sub> is highly sensitive to environmental fluctuations, hampering interpretation and reducing reproducibility.</jats:list-item> <jats:list-item>We developed a simple and novel correction factor (named DynG) for <jats:italic>I</jats:italic><jats:sub>g</jats:sub> that accounts for environmental fluctuations when scaling <jats:italic>I</jats:italic><jats:sub>g</jats:sub> to <jats:italic>g</jats:italic><jats:sub>sw</jats:sub>. This was achieved by capturing temperature variations in a set of ALs with a range of known constant pore conductances. This approach provided the <jats:italic>I</jats:italic><jats:sub>g</jats:sub>–conductance relationship, using ALs as a reference, to infer <jats:italic>g</jats:italic><jats:sub>sw</jats:sub> of real leaves from their measured <jats:italic>I</jats:italic><jats:sub>g</jats:sub>.</jats:list-item> <jats:list-item>In fluctuating environments, <jats:italic>g</jats:italic><jats:sub>sw</jats:sub> estimated using DynG showed greater accuracy and stability than <jats:italic>g</jats:italic><jats:sub>sw</jats:sub> calculated from <jats:italic>I</jats:italic><jats:sub>g</jats:sub> alone, and was in good agreement with <jats:italic>g</jats:italic><jats:sub>sw</jats:sub> determined using lysimetric and gas exchange methods. DynG's power was further showcased in distinguishing <jats:italic>g</jats:italic><jats:sub>sw</jats:sub> of Arabidopsis genotypes differing in stomatal traits (Col‐0, <jats:italic>epf1epf2</jats:italic>, and EPF2OE).</jats:list-item> <jats:list-item>We conclude that <jats:italic>I</jats:italic><jats:sub>g</jats:sub> corrected with DynG can reliably estimate <jats:italic>g</jats:italic><jats:sub>sw</jats:sub> in fluctuating environments without complex modeling, opening new avenues for <jats:italic>g</jats:italic><jats:sub>sw</jats:sub> phenotyping and monitoring.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"53 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145002874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic architecture of the S‐locus supergene revealed in a tetraploid distylous species","authors":"Zhonglai Luo, Spencer C. H. Barrett, Tieyao Tu, Zhongtao Zhao, Shanshan Jia, Shiran Gu, Tingting Duan, Yu Zhang, Bingqiang Xu, Lei Gu, Xiaofang Deng, Libo Jiang, Miaomiao Shi, Dianxiang Zhang","doi":"10.1111/nph.70521","DOIUrl":"https://doi.org/10.1111/nph.70521","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Heterostyly is a polymorphic floral adaptation controlled by supergenes. The molecular basis of distyly has been investigated in diploid species from several unrelated families, but information is lacking for polyploid systems.</jats:list-item> <jats:list-item>Here, we address this knowledge gap in <jats:italic>Schizomussaenda henryi</jats:italic>, a tetraploid distylous species of Rubiaceae, the family with the greatest number of heterostylous species. Using chromosome‐level genome assemblies and transcriptome profiling, we characterized its tetraploid genome, identified the <jats:italic>S</jats:italic>‐locus region, and performed evolutionary analysis.</jats:list-item> <jats:list-item>The <jats:italic>S</jats:italic>‐locus contains four hemizygous genes in the S‐morph. <jats:italic>SchzAUX22</jats:italic> emerged as a candidate gene potentially regulating both style length and filament growth via auxin signaling. Phylogenetic and <jats:italic>k</jats:italic>‐mer‐based analysis suggested a hybrid allopolyploid origin for <jats:italic>S. henryi</jats:italic>, while no subgenome dominance was detected. Results from the comparison of <jats:italic>K</jats:italic><jats:sub><jats:italic>s</jats:italic></jats:sub> values indicated that <jats:italic>S</jats:italic>‐locus formation likely occurred through stepwise duplications.</jats:list-item> <jats:list-item>This study provides the first comprehensive genomic analysis of distyly in a polyploid species and demonstrates that the <jats:italic>S</jats:italic>‐locus remains intact despite allopolyploidization resulting from hybridization. Our results indicated that polyploidization does not necessitate the breakdown of distyly, which occurs in several other heterostylous lineages.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"53 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145002875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid epigenome unveils parental genetic divergence shaping salt-tolerant heterosis in Brassica napus","authors":"Junlin Chen,&nbsp;Minyan Zhang,&nbsp;Qing Zhang,&nbsp;Xianfei Hou,&nbsp;Donghai Jia,&nbsp;Yuanguo Gu,&nbsp;Heping Wan,&nbsp;Hu Zhao,&nbsp;Jing Wen,&nbsp;Bin Yi,&nbsp;Tingdong Fu,&nbsp;Jinxiong Shen,&nbsp;Lun Zhao","doi":"10.1111/nph.70532","DOIUrl":"10.1111/nph.70532","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Heterosis holds great potential for improving yield, quality, and environmental adaptability in crop breeding, which suggests that hybrids can exhibit better performance in adapting to extreme environments. However, the epigenetic mechanisms of salt-tolerant heterosis in allopolyploid crop <i>Brassica napus</i> (AACC, 2<i>n</i> = 38), particularly chromatin accessibility, remain largely unexplored.</li>\u0000 \u0000 <li>We investigated the dynamics of chromatin accessibility and transcriptional reprogramming during a time course of salt exposure in <i>Brassica napus</i> hybridization. We observed the importance of epigenetic and transcriptional regulation in plant resilience. The chromatin accessibility and transcriptome rapidly changed within a short time frame of salt exposure.</li>\u0000 \u0000 <li>Hybrid possessed more accessible chromatin and more active transcriptome than that of parents driven by epigenetic aggregation and genetic complementation. Broader and more flexible genomic resources enabled hybrid preferentially unitized advantageous alleles for salt stress adaptation. Meanwhile, these salt stress-responsive genes in hybrid exerted various heterotic effects, with non-additive genetic effects, including full-dominance, partial-dominance, and overdominance effects, playing a crucial role in salt stress adaptation.</li>\u0000 \u0000 <li>Our results expanded the heterosis hypothesis from an epigenetic perspective and emphasized how the combined effects of genetic and epigenetic factors enable hybrid to better withstand salt stress.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"248 3","pages":"1475-1490"},"PeriodicalIF":8.1,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of rhizobial cheaters by the host Medicago truncatula involves repression of symbiotic functions and induction of defense","authors":"Min Chen,&nbsp;Axelle Raisin,&nbsp;Natalie Judkins,&nbsp;Pierre-Marie Allard,&nbsp;Emmanuel Défossez,&nbsp;Michael Stumpe,&nbsp;Inmaculada Yruela,&nbsp;Manuel Becana,&nbsp;Didier Reinhardt","doi":"10.1111/nph.70494","DOIUrl":"10.1111/nph.70494","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"248 3","pages":"1458-1474"},"PeriodicalIF":8.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144995670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constraint-based metabolic modeling reveals metabolic properties underpinning the unprecedented growth of Chlorella ohadii","authors":"Fayaz Soleymani,&nbsp;Sandra Marcela Correa,&nbsp;Marius Arend,&nbsp;Niayesh Forghanisardaghi,&nbsp;Haim Treves,&nbsp;Zahra Razaghi-Moghadam,&nbsp;Zoran Nikoloski","doi":"10.1111/nph.70528","DOIUrl":"10.1111/nph.70528","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"248 3","pages":"1572-1583"},"PeriodicalIF":8.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.70528","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145002876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue humidity and pH as important species traits regulating tree methane emissions in floodplain wetland forests","authors":"Marie‐Ange Moisan, Vincent Maire, Jacob Isabelle, Didier Philippo, Christine Martineau","doi":"10.1111/nph.70543","DOIUrl":"https://doi.org/10.1111/nph.70543","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>Despite the increasing number of studies investigating tree methane fluxes, the relationships between tree methane fluxes and species traits remain mostly unexplored.</jats:list-item> <jats:list-item>We measured leaf and stem methane fluxes of five tree species (<jats:italic>Acer saccharinum</jats:italic>, <jats:italic>Fraxinus nigra</jats:italic>, <jats:italic>Ulmus americana</jats:italic>, <jats:italic>Salix nigra</jats:italic>, and <jats:italic>Populus</jats:italic> spp.) in the floodplain of Lake St‐Pierre (Québec) and examined how these fluxes vary with species traits (wood density, humidity, pH; leaf water content, pH, stomatal conductance; methanogen and methanotroph relative abundances (RAs) in leaf, wood, and bark).</jats:list-item> <jats:list-item>Tree methane fluxes differed among tree species according to traits linked to the transport of soil‐produced methane and chemical conditions associated with the regulation of methane‐cycling microorganisms. Tree fluxes were correlated positively with heartwood and leaf pH and negatively with their humidity. Stem emissions were positively correlated with methanogen RA in heartwood, and leaf emissions were negatively correlated with the RA of leaf epiphytic methanotrophs, suggesting a contribution of tree microbiota in the regulation of methane fluxes.</jats:list-item> <jats:list-item>We demonstrated for the first time that tissue pH may be a particularly important trait influencing tree methane fluxes via the regulation of microbial mechanisms. Species with low tissue pH show potential for methane release mitigation.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"28 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144995154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic diversity in the genus Blepharis (Acanthaceae) and the evolutionary origins of C4 photosynthesis","authors":"Matheus E. Bianconi, Colin P. Osborne, Luke T. Dunning","doi":"10.1111/nph.70541","DOIUrl":"https://doi.org/10.1111/nph.70541","url":null,"abstract":"","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"13 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144995153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distal to proximal: a continuum of drivers shaping tree growth and carbon partitioning","authors":"Antoine Cabon","doi":"10.1111/nph.70516","DOIUrl":"https://doi.org/10.1111/nph.70516","url":null,"abstract":"SummaryThe relationship between tree carbon (C) assimilation and growth is central to understanding tree functioning and forecasting forest C sequestration, yet remains unresolved. The long‐standing debate over C <jats:italic>source</jats:italic> vs <jats:italic>sink</jats:italic> limits to growth has yielded invaluable insight, but rests on a false dichotomy. Reframing this issue in terms of <jats:italic>distal</jats:italic>‐<jats:italic>to</jats:italic>‐<jats:italic>proximal</jats:italic> processes driving sink activity and placing it within a broader understanding of C partitioning offers new insights. Building on transport‐resistance theory, I outline a framework where plant resource economies shape spatial gradients of resource availability along the leaf‐to‐root axis, thereby regulating local sink activity. This spatially explicit, trait‐informed perspective aligns with optimality theory and provides a mechanistic link between C partitioning and the plant functional trait spectrum. By moving beyond binary limitations and emphasizing integrated physiological processes, this approach can improve understanding of tree function and biomass increment under climate change.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"34 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144995157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-frequency sampling unveils biotic and abiotic drivers of rapid phytoplankton morphological changes.","authors":"Pavel Škaloud,Kateřina Tučková,Radka Čablová,Iva Jadrná,Ivana Černajová","doi":"10.1111/nph.70534","DOIUrl":"https://doi.org/10.1111/nph.70534","url":null,"abstract":"Phytoplankton, as primary producers, play a key role in aquatic ecosystems. Their community turnover is shaped by morphological traits that enable adaptation to diverse abiotic and biotic factors. Yet, the temporal scale of these dynamics remains poorly understood due to limited high-frequency sampling studies. Employing DNA metabarcoding, we assessed the community composition of the phytoplankton lineage Synurales (Chrysophyceae) at 3-d intervals during 70 d at a shallow peat bog lake in the Czech Republic. The selected group possesses a variety of species-specific key morphological traits, such as cell size, coloniality, and bristle formation. Using a custom reference database of cultured species, we assigned 99.93% of eDNA reads to 74 species-level lineages with known morphological traits. Community changes in colonial species were influenced by abiotic drivers such as silica concentration and wind speed. By contrast, shifts in unicellular species communities were mainly driven by Cladocera predators, influencing the occurrence of bristle-bearing species. Changes in species composition and morphological traits occurred within days, mirroring environmental variability. Achieving such fine-scale resolution, especially for small or rare taxa, would be extremely difficult using microscopy alone. eDNA enabled high-resolution community profiling and abundance estimation, demonstrating its key role and the importance of comprehensive reference databases.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"30 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144995840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorylation of the transcription factor PoWRKY31 by PoKIN10 activates lignin biosynthesis to improve tree peony drought tolerance","authors":"Yuting Luan, Heng Yu, Honglei An, Zijie Chen, Daqiu Zhao, Jun Tao","doi":"10.1111/nph.70546","DOIUrl":"https://doi.org/10.1111/nph.70546","url":null,"abstract":"Summary<jats:list list-type=\"bullet\"> <jats:list-item>The SNF1‐Related Protein Kinase 1 (SnRK1) signaling cascade is highly conserved and plays a crucial role in coordinating various cellular processes in plants. However, its regulatory mechanisms in stress responses remain largely unclear.</jats:list-item> <jats:list-item>Here, we started from the perspectives of transcriptional regulation, protein–protein interaction, and posttranslational modification, and through the integration of multiple molecular biology approaches and functional validation methods, we conducted a detailed analysis of the mechanisms by which PoKIN10 (SnRK1 catalytic subunit), PoWRKY31, PoWRKY75, and PoCCoAOMT participate in drought tolerance in tree peony.</jats:list-item> <jats:list-item>We report a novel regulatory module that modulates lignin biosynthesis to enhance drought tolerance in tree peony. PoKIN10 acts as a key regulator of lignin‐mediated drought tolerance by phosphorylating PoWRKY31 at the Ser262 residue, thereby enhancing its protein stability, DNA‐binding affinity, and transcriptional activation activity. PoWRKY31 directly binds to the promoter of <jats:italic>PoWRKY75</jats:italic> and activates its expression, which subsequently induces <jats:italic>PoCCoAOMT</jats:italic> expression, leading to increased lignin accumulation, improved water retention, and enhanced reactive oxygen species (ROS) scavenging capacity under drought stress.</jats:list-item> <jats:list-item>We therefore elucidate a regulatory module that governs lignin biosynthesis to improve drought tolerance in tree peony, offering new insights into lignin‐mediated drought adaptation in plants.</jats:list-item> </jats:list>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"15 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144995229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}