New Phytologist最新文献

{"title":"Pinpointing the timing of meiosis: a critical factor in evaluating the impact of abiotic stresses on the fertility of cereal crops","authors":"Farhad Masoomi‐Aladizgeh, Brian J. Atwell, Anowarul I. Bokshi, Rebecca J. Thistlethwaite, Ali Khoddami, Richard Trethowan, Daniel K. Y. Tan, Thomas H. Roberts","doi":"10.1111/nph.20297","DOIUrl":"https://doi.org/10.1111/nph.20297","url":null,"abstract":"SummaryThe development of male gametes, vital to sexual reproduction in crops, requires meiosis followed by successive mitotic cell divisions of haploid cells. The formation of viable pollen is especially vulnerable to abiotic stress, with consequences both for yield and for grain quality. An understanding of key molecular responses when specific stages during pollen development are subjected to stress (e.g. heat) is possible only when sampling is carefully informed by developmental biology. Traditionally, morphological characteristics have been commonly used in cereals as ‘indicators’ of male reproductive stages. We argue that these morphological attributes are strongly influenced by genotype and genotype–environment interactions and cannot be used reliably to define developmental events during microsporogenesis and microgametogenesis. Furthermore, asynchronous development along the axis of a single inflorescence calls for selective sampling of individual florets to define specific reproductive stages accurately. We therefore propose guidelines to standardise the sampling of cells during male reproductive development, particularly when interrogating the impact of stress on susceptible meiosis. Improved knowledge of development will largely negate the variability imposed by genotype, environment and asynchronous development of florets. Highlighting the subtleties required for sampling and investigation of male reproductive stages will make the selection of abiotic stress‐tolerant cereal genotypes more reliable.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"25 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transcription factor PpRKD evokes female developmental fate in the sexual reproductive organs of Physcomitrium patens","authors":"Emiko Yoro,&nbsp;Seiya Suzuki,&nbsp;Nobuhiro Akiyoshi,&nbsp;Rumiko Kofuji,&nbsp;Keiko Sakakibara","doi":"10.1111/nph.20262","DOIUrl":"10.1111/nph.20262","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"653-667"},"PeriodicalIF":8.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20262","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142689315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracing phosphorus from soil through mycorrhizal fungi to plants","authors":"Ylva Lekberg,&nbsp;Jan Jansa,&nbsp;David Johnson,&nbsp;Paul Milham,&nbsp;Chad Penn,&nbsp;Benjamin P. Colman","doi":"10.1111/nph.20217","DOIUrl":"10.1111/nph.20217","url":null,"abstract":"&lt;p&gt;A recent paper about carbon and phosphorus (P) transfer between arbuscular mycorrhizal (AM) fungi and plants (Lekberg &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;) was cited by Spohn &amp; Wanek (&lt;span&gt;2025&lt;/span&gt;; pp. 443–445, in this issue) to highlight potential pitfalls of using P radioisotopes (in this case &lt;sup&gt;32&lt;/sup&gt;P, although equally relevant for &lt;sup&gt;33&lt;/sup&gt;P as they behave similarly; Frossard &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2011&lt;/span&gt;). Specifically, the paper by Spohn &amp; Wanek (&lt;span&gt;2025&lt;/span&gt;) states that without knowing the specific activity in soil solution (i.e. the ratio of &lt;sup&gt;32&lt;/sup&gt;P : &lt;sup&gt;31&lt;/sup&gt;P), the conclusion in Lekberg &lt;i&gt;et al&lt;/i&gt;. (&lt;span&gt;2024&lt;/span&gt;) that more P was delivered by AM fungi in high-P than low-P soils may not be valid if the three low-P soils sorbed more &lt;sup&gt;32&lt;/sup&gt;P. We agree with Spohn &amp; Wanek (&lt;span&gt;2025&lt;/span&gt;) that tracer experiments should be interpreted with caution, and we appreciate the opportunity to explore some of the pitfalls highlighted in that paper in more detail.&lt;/p&gt;&lt;p&gt;Sorption of inorganic orthophosphate (H&lt;sub&gt;2&lt;/sub&gt;PO&lt;sub&gt;4&lt;/sub&gt;&lt;sup&gt;−&lt;/sup&gt; and HPO&lt;sub&gt;4&lt;/sub&gt;&lt;sup&gt;2−&lt;/sup&gt;) – the main P form taken up by plants, fungi, and prokaryotes from soil solution (Bucher, &lt;span&gt;2007&lt;/span&gt;) – is determined by pH, organic matter, hydrous oxides of aluminum and iron, and calcium carbonate (Frossard &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;1995&lt;/span&gt;; Daly &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2001&lt;/span&gt;; Barrow, &lt;span&gt;2017&lt;/span&gt;). In Lekberg &lt;i&gt;et al&lt;/i&gt;. (&lt;span&gt;2024&lt;/span&gt;), soils were collected from two regions, one with high-P availability and one with low-P availability. In both regions, soils were classified as fine to gravely, loamy Mollisols, and neither soil pH (6.80 ± 0.38 vs 6.53 ± 0.38, means ± SE) nor organic matter concentrations (4.50 ± 0.69% and 5.03 ± 2.00%) differed (&lt;i&gt;P&lt;/i&gt; &gt; 0.5) between the high-P and low-P soils, respectively. Total organic P stocks also did not differ significantly between the high-P and low-P soils (1178 ± 302 and 684 ± 79 mg kg&lt;sup&gt;−1&lt;/sup&gt;) and the strong regional difference in P availability based on Bray 1 extractions were likely due to soil mineralogy.&lt;/p&gt;&lt;p&gt;There is another reason why differences in sorption likely did not influence results in Lekberg &lt;i&gt;et al&lt;/i&gt;. (&lt;span&gt;2024&lt;/span&gt;). One of the main assumptions of plant–soil–isotope experiments is that the concentration of the isotope-tracer must be negligible compared to that of the nonlabelled nutrient in solution. If the amount of &lt;sup&gt;32&lt;/sup&gt;P added is similar to &lt;sup&gt;31&lt;/sup&gt;P, the solution and solid-phase P equilibrium is disrupted and net sorption of total and &lt;sup&gt;32&lt;/sup&gt;P will occur. The solution concentration where no net sorption or desorption occurs in soil is known as the ‘equilibrium P concentration at net zero sorption’ (EPC&lt;sub&gt;0&lt;/sub&gt;). Specific to each soil, EPC&lt;sub&gt;0&lt;/sub&gt; depends on the same variables that determine sorption, as well as P desorption kinetics, and microbial P immobilization and release. A rec","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"446-449"},"PeriodicalIF":8.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142689316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-based identification of general transcriptional predictors for plant disease","authors":"Jayson Sia,&nbsp;Wei Zhang,&nbsp;Mingxi Cheng,&nbsp;Paul Bogdan,&nbsp;David E. Cook","doi":"10.1111/nph.20264","DOIUrl":"10.1111/nph.20264","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>This study investigated the generalizability of <i>Arabidopsis thaliana</i> immune responses across diverse pathogens, including <i>Botrytis cinerea</i>, <i>Sclerotinia sclerotiorum</i>, and <i>Pseudomonas syringae</i>, using a data-driven, machine learning approach.</li>\u0000 \u0000 <li>Machine learning models were trained to predict disease development from early transcriptional responses. Feature selection techniques based on network science and topology were used to train models employing only a fraction of the transcriptome. Machine learning models trained on one pathosystem where then validated by predicting disease development in new pathosystems.</li>\u0000 \u0000 <li>The identified feature selection gene sets were enriched for pathways related to biotic, abiotic, and stress responses, though the specific genes involved differed between feature sets. This suggests common immune responses to diverse pathogens that operate via different gene sets.</li>\u0000 \u0000 <li>The study demonstrates that machine learning can uncover both established and novel components of the plant's immune response, offering insights into disease resistance mechanisms. These predictive models highlight the potential to advance our understanding of multigenic outcomes in plant immunity and can be further refined for applications in disease prediction.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"785-806"},"PeriodicalIF":8.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142689352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis reveals globally sourced commercial mycorrhizal inoculants fall short","authors":"Liz Koziol, Thomas P. McKenna, James D. Bever","doi":"10.1111/nph.20278","DOIUrl":"https://doi.org/10.1111/nph.20278","url":null,"abstract":"&lt;h2&gt; Introduction&lt;/h2&gt;\u0000&lt;p&gt;Several researchers have highlighted the potential of microbial inoculants to advance sustainable agriculture (Elnahal &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;; O'Callagha&lt;i&gt;n et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;). Among microbial inoculants, arbuscular mycorrhizal (AM) fungi have garnered attention for their ability to enhance soil health and plant fitness. AM fungi can increase plant growth through enhanced access to limiting soil resources, improve plant defense against herbivores and pathogens, increase tolerance to drought and salinity stress, and increase carbon sequestration (Reynolds &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2006&lt;/span&gt;; Bennett &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2009&lt;/span&gt;; Ji &amp; Bever, &lt;span&gt;2016&lt;/span&gt;). With this promise, the commercial market for AM inoculants is rapidly growing, approaching 995 million USD globally (Mordor Intelligence, &lt;span&gt;2024&lt;/span&gt;). AM inoculants, often referred to as ‘endomycorrhizal’ inoculants on commercial product labels, are easily and widely available in many regions of the world.&lt;/p&gt;\u0000&lt;p&gt;Despite the optimism surrounding microbial inoculants, global studies have revealed inconsistencies with commercial products, including instances of crop mortality, unlabeled fertilizers, and nonviability (Corkidi &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2004&lt;/span&gt;; Tarbell &amp; Koske, &lt;span&gt;2007&lt;/span&gt;; Faye &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2013&lt;/span&gt;; Duell &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;; M. Salomon &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;; Koziol &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;). The benefits of commercial products can be limited by their narrow inclusion of the same four to five species, with many containing a single AM fungus in the &lt;i&gt;Rhizophagus&lt;/i&gt; genus (Basiru &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2020&lt;/span&gt;), despite evidence that a more diverse AM fungal consortium may increase crop growth (Magnoli &amp; Bever, &lt;span&gt;2023&lt;/span&gt;), nutrient uptake (Reynolds &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2006&lt;/span&gt;), and other benefits. Concerns regarding product mislabeling and contamination by fungal pathogens further highlight the potential risks associated with these products (Tarbell &amp; Koske, &lt;span&gt;2007&lt;/span&gt;; Vahter &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2023&lt;/span&gt;). The lack of accountability for product viability is compounded by scientific assessments that often do not report product identities, although some do (Wiseman &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2009&lt;/span&gt;; Faye &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2020&lt;/span&gt;), making it a challenge for both the inoculant industry and for users to be informed on product quality concerns. Regulatory frameworks for mycorrhizal inoculants remain limited in many regions (Carrazco &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;; M. J. Salomon &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2022&lt;/span&gt;), exacerbating challenges related to product viability and identity of mycorrhizal fungi in products. The United States fully lacks regulations on the import/export of mycorrhizal fungal products or quality control, despite the United States having a 25% share in the mycorrhizal inoculant industry, representing 249 million","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"19 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying element fluxes using radioisotopes","authors":"Marie Spohn,&nbsp;Wolfgang Wanek","doi":"10.1111/nph.20203","DOIUrl":"10.1111/nph.20203","url":null,"abstract":"&lt;p&gt;Radioisotopes can be used to quantify element fluxes in ecosystems, such as plant phosphorus uptake from soil. On the occasion of a recent publication (Lekberg &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2024&lt;/span&gt;), this article briefly explains some challenges in the determination of element fluxes based on radioisotope labeling experiments along with strategies to avoid potential pitfalls. The intention of this contribution is to foster progress in the understanding of element fluxes in ecosystems based on the use of isotopes.&lt;/p&gt;&lt;p&gt;Radioisotopes can be used in quantitative and nonquantitative studies (for a review, see Frossard &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;2011&lt;/span&gt;). In nonquantitative studies, radioisotopes are often used to demonstrate that specific elements or molecules move among different compartments, for instance among cells or organs. Using this approach, it has been shown that mycorrhizal fungi transport elements from soil or a specific soil compartment to a plant. By contrast, other studies use radioisotopes to quantify the magnitude of an element flux. In these quantitative studies, the radioisotope is used as a tracer (i.e. a traceable proportion of the element in the studied system).&lt;/p&gt;&lt;p&gt;If an isotope is used as a tracer to quantify an element flux, rather than the flux of the tracer itself, it is essential to know the ratio of the amount of this isotope to the total amount of the element in the labeled pool (for a review see Di &lt;i&gt;et al&lt;/i&gt;., &lt;span&gt;1997&lt;/span&gt;). This is not a unique precondition in the use of radioisotopes. The same applies also when stable isotopes are used to trace fluxes. The difference is that radioisotopes are determined based on their radioactivity (for instance, &lt;sup&gt;32&lt;/sup&gt;P activity) using scintillation counting, while stable isotopes are determined as the ratio of the added heavy isotope relative to the abundant light isotope of the element (for instance, the &lt;sup&gt;15&lt;/sup&gt;N : &lt;sup&gt;14&lt;/sup&gt;N ratio) using isotope ratio mass spectrometry. Thus, when using radioisotopes to trace element fluxes, it is necessary to determine not only the amount of the radioisotope (based on its radioactivity) but also the amount of the nonlabeled (or total) element in the system, in separate measurements.&lt;/p&gt;&lt;p&gt;If radioactive phosphorus, for instance &lt;sup&gt;32&lt;/sup&gt;P, is added to a soil as phosphate, a large part of it will adsorb to soil minerals, while the remaining part will be taken up by microorganisms. The fraction of &lt;sup&gt;32&lt;/sup&gt;P that remains plant-available in the soil (which can be as little as 1% of the added amount) will be strongly diluted by nonlabeled phosphorus (for a review see Bünemann, &lt;span&gt;2015&lt;/span&gt;). The plant will take up the radioisotope together with nonlabeled phosphorus from the plant-available pool, and the ratio of radiophosphorus : nonlabeled phosphorus (called specific activity) that is taken up can vary strongly among soils (Fig. 1). Hence, the amount of radioisotope in the plant by itself has only limited value ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"443-445"},"PeriodicalIF":8.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142689314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbicidal interference: glyphosate drives both the ecology and evolution of plant–herbivore interactions","authors":"Grace M. Zhang,&nbsp;Regina S. Baucom","doi":"10.1111/nph.20238","DOIUrl":"10.1111/nph.20238","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"807-817"},"PeriodicalIF":8.3,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20238","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matthew Naish","authors":"","doi":"10.1111/nph.20281","DOIUrl":"10.1111/nph.20281","url":null,"abstract":"&lt;p&gt;My interest in plant science grew from a general fascination with science and deepened through hands-on lab experiences. It began with my high school work experience, where I spent two weeks in a plant physiology lab at Lancaster University. This sparked my curiosity about plant biology and inspired me to explore the field further. The following year, I received a Nuffield bursary, which allowed me to work on a five-week project studying the ecology of predator–prey interactions across different environments. This project gave me a deeper appreciation for the role plants play in these systems.&lt;/p&gt;&lt;p&gt;As I continued my studies, I became increasingly interested in the remarkable adaptability and diversity of plants. Unlike animals, plants must dynamically adjust to their surroundings, acting as incredible ‘factories’ that must adapt in changing environments. Learning about the biological processes that enable this adaptability truly hooked me, and my passion for plant science has only grown since then!&lt;/p&gt;&lt;p&gt;The simple answer is that I enjoy it; research captivates me in a way nothing else does. I appreciate the freedom this career offers to pursue my own research topics and the variety of people and students I get to work with. During my Master's in Sustainable Agriculture and Food Security, which included non-science modules on environmental management and law, I realised that the area I was most engaged with was the research project.&lt;/p&gt;&lt;p&gt;My introduction to epigenetics sparked my desire to pursue a PhD. I was captivated by the intricate mechanisms cells use to regulate genes, allowing them to perform specialised roles despite sharing the same genetic code. This interest led me to explore how these mechanisms work, with the aim of developing new tools to support sustainable agriculture and food security in the face of climate change.&lt;/p&gt;&lt;p&gt;At its root, my motivation is curiosity, and this job gives me the opportunity to continually learn. I really enjoy problem solving and using technological developments to investigate questions in new ways. I am also motivated by the people I work with, discussing new bits of data and new ideas with colleagues in the lab is one of the best parts of the job.&lt;/p&gt;&lt;p&gt;I have been fortunate to have had great advisors who have substantially contributed to my growth as a scientist. My academic mentors, Ian Dodd (Lancaster University, UK), Jose Gutierrez-Marcos (University of Warwick, UK), and Ian Henderson (University of Cambridge, UK), stand out as significant role models. Beyond the concrete skills and knowledge they offered, they have shaped my approach to science, such as how to frame a compelling research question, identify big questions in the field, and develop effective strategies for writing grant proposals and research papers. Their support and encouragement have had a profound impact on my growth as a researcher, and I deeply admire their continued enthusiasm for their work. I am very grateful for their g","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"244 6","pages":"2141-2142"},"PeriodicalIF":8.3,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nuclear phylogenomic tree of grasses (Poaceae) recovers current classification despite gene tree incongruence","authors":"Grass Phylogeny Working Group III","doi":"10.1111/nph.20263","DOIUrl":"10.1111/nph.20263","url":null,"abstract":"<p>\u0000 </p>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"818-834"},"PeriodicalIF":8.3,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/nph.20263","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant PI4P is required for bacteria to translocate type-3 effectors","authors":"Jinfeng Peng,&nbsp;Liyuan Zhang,&nbsp;Kai Lu,&nbsp;Xiaochen Chen,&nbsp;Hao Pang,&nbsp;Xiaohui Yao,&nbsp;Ping Li,&nbsp;Peng Cao,&nbsp;Xiaoxu Li,&nbsp;Zuodong Wang,&nbsp;Lina Qin,&nbsp;Miao Zhou,&nbsp;Maoling Wang,&nbsp;Qizhen Li,&nbsp;Chunyu Qiu,&nbsp;Mingxin Sun,&nbsp;Yufen Li,&nbsp;Liping Gong,&nbsp;Xinlin Wei,&nbsp;Siyi Wang,&nbsp;Jiajia Chen,&nbsp;Chongchong Lu,&nbsp;Shenshen Zou,&nbsp;Xinhua Ding,&nbsp;Lei Chen,&nbsp;Meixiang Zhang,&nbsp;Hansong Dong","doi":"10.1111/nph.20248","DOIUrl":"10.1111/nph.20248","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 </p><ul>\u0000 \u0000 <li>Type-3 effectors (T3E) of phytopathogenic Gram-negative bacteria fulfill a virulent role, causing disease, or an avirulent role, inducing immunity, following their translocation into plant cells. This study aimed to validate the hypothesis that bacterial T3E translocation requires lipidic compounds in plant cell membranes.</li>\u0000 \u0000 <li>Based on genetic, molecular, and biochemical assays, we determined that phosphatidylinositol 4-phosphate (PI4P) associated with plant cell membranes is essential for the translocation of T3E by bacterial pathogens.</li>\u0000 \u0000 <li>Replicate experimental data revealed that PI4P cooperates with the type-3 translocase HrpF to facilitate the translocation of effectors TAL and Xop from <i>Xanthomonas oryzae</i> and Hop from <i>Pseudomonas syringae</i> into the cells of <i>Oryza sativa</i> and <i>Nicotiana benthamiana</i>, respectively. Genetic and molecular analyses confirmed that, once translocated into plant cells, the distinct effectors induce disease or immunity. Combined genetic and pharmacological analyses revealed that when PI4P content is suppressed via genetic or pharmacological measures, the T3 effector translocation is considerably suppressed, resulting in serious inhibition of bacterial infection.</li>\u0000 \u0000 <li>Overall, these findings demonstrate that cooperative functioning of HrpF–PI4P is conserved in bacterial effectors and plants.</li>\u0000 </ul>\u0000 </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"245 2","pages":"748-766"},"PeriodicalIF":8.3,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}