New Phytologist最新文献_第8页

The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-03-06 DOI: 10.1111/nph.70019

Katarina Kurtović, Stanislav Vosolsobě, Daniel Nedvěd, Karel Müller, Petre Ivanov Dobrev, Vojtěch Schmidt, Piotr Piszczek, Andre Kuhn, Adrijana Smoljan, Tom J. Fisher, Dolf Weijers, Jiří Friml, John L. Bowman, Jan Petrášek

{"title":"The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii","authors":"Katarina Kurtović, Stanislav Vosolsobě, Daniel Nedvěd, Karel Müller, Petre Ivanov Dobrev, Vojtěch Schmidt, Piotr Piszczek, Andre Kuhn, Adrijana Smoljan, Tom J. Fisher, Dolf Weijers, Jiří Friml, John L. Bowman, Jan Petrášek","doi":"10.1111/nph.70019","DOIUrl":"https://doi.org/10.1111/nph.70019","url":null,"abstract":"<h2> Introduction</h2>\u0000During the transition from water to land, plants underwent a series of developmental innovations, leading to the establishment of a complex body (Harrison, 2017; Donoghue et al., 2021; Bowman, 2022). A key regulator of land plant development is the phytohormone auxin, which acts through local biosynthesis and directional transport, resulting in the formation of concentration gradients (Friml, 2022). The directional flow of auxin, facilitated by the PIN family of auxin efflux carriers, is critical for plant morphogenesis and adaptive growth responses to environmental cues (Gao et al., 2008; Luschnig & Friml, 2024). Recent research has provided significant insights into the mechanisms of auxin action (Kuhn et al., 2024), including the structural elucidation of three PIN auxin efflux carriers (Su et al., 2022; Ung et al., 2022; Yang et al., 2022). However, the question of how and when auxin became a pivotal driver of morphological changes in land plants remains largely unanswered. This question cannot be fully addressed by studying only land plants but requires a comprehensive investigation of their algal relatives (Skokan et al., 2019). Land plants (embryophytes) and streptophyte green algae, from which they emerged, together form the group Streptophyta (Becker & Marin, 2009). Streptophyte algae comprise six clades, exhibiting significant morphological diversity within these clades (Buschmann, 2020; Bierenbroodspot et al., 2024). Among these six clades, Chara spp. and Nitella spp., members of the Charophyceae family, possess the highest degree of complexity with respect to their body plan. Due to its large internodal cells, transparent gravitropic rhizoids, and rapid cytoplasmic streaming, Chara has been a model organism for decades, facilitating the study of fundamental cell biological processes (Kurtović et al., 2024).\u0000Although genome sequencing revealed that Chara braunii lacks the auxin biosynthetic pathway involving the TAA and YUCCA genes, which converts tryptophan to an auxin, indole-3-acetic acid (IAA) (Nishiyama et al., 2018), earlier and recent studies are consistently confirming the presence of IAA in the biomass of various species of Chara and Nitella (Jahnke & Libbert, 1964; Sztein et al., 2000; Hackenberg & Pandey, 2014; Beilby et al., 2015; Schmidt et al., 2024), suggesting the presence of an alternative biosynthetic pathway. Besides IAA, other phytohormones have been identified in the biomass of C. braunii, including cytokinin N6-(∆2-iso","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"7 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143560686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multifunctional organelle coordinates phagocytosis and chlorophagy in a marine eukaryote phytoplankton Scyphosphaera apsteinii 一种多功能细胞器协调海洋真核浮游植物 Scyphosphaera apsteinii 的吞噬作用和噬氯作用

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-03-04 DOI: 10.1111/nph.20388

Julie A. Koester, Oren Fox, Elizabeth Smith, Madison B. Cox, Alison R. Taylor

{"title":"A multifunctional organelle coordinates phagocytosis and chlorophagy in a marine eukaryote phytoplankton Scyphosphaera apsteinii","authors":"Julie A. Koester, Oren Fox, Elizabeth Smith, Madison B. Cox, Alison R. Taylor","doi":"10.1111/nph.20388","DOIUrl":"https://doi.org/10.1111/nph.20388","url":null,"abstract":"<h2> Introduction</h2>\u0000Haptophyte microalgae, including biomineralizing coccolithophores and naked flagellates, comprise 30–50% of the standing stock of microbial primary producers in the world's ocean (Liu et al., 2009). As mixotrophs, haptophyte flagellates are responsible for, on average, 40% of bacterivory in oligotrophic ecosystems (Unrein et al., 2014). Mixotrophy is a collection of functional physiological traits defined by a combination of autotrophic and phagotrophic carbon acquisition (Raven et al., 2009; Flynn et al., 2019) distinct from osmotrophy, which is the uptake of dissolved organic molecules (Flynn et al., 2013). Mixoplankton are important drivers of carbon flow in pelagic microbial communities (Mitra et al., 2014, 2016; Ward & Follows, 2016), with significant contributions to bacterivory and carbon transfer by pico- and nanoplankton (Zubkov & Tarran, 2008). Mixotrophy also allows for life-history, phenotypic, and habitat flexibility, including the ability to thrive in oligotrophic regions or survive subphotic conditions and periods of darkness (Brutemark & Granéli, 2011; Anderson et al., 2018; Wilken et al., 2020). Experimental and modeling studies suggest mixotrophy traits, including increased phagotrophy, may be favored under ocean warming scenarios, for example if metabolic rate responses of phagotrophy are greater than those of photosynthesis (Gonzalez et al., 2022; Lepori-Bui et al., 2022).\u0000Studies directly testing phagocytosis in haptophytes have been conducted primarily on motile representatives (Anderson et al., 2018), including 3 species from the calcifying subclass Calcihaptophycidae (de Vargas et al., 2007), the coccolithophores (Parke & Adams, 1960; Houdan et al., 2006; Avrahami & Frada, 2020). In addition to two flagella, motile haptophytes have a characteristic haptonema, a unique microtubule-based appendage located between the flagella. The haptonema intercepts small prey particles in the flagella-driven feeding currents and deposits them on the posterior portion of the cell, opposite the flagellar and haptonemal roots, where phagocytosis occurs (Parke & Adams, 1960; Kawachi et al., 1991; Kawachi & Inouye, 1995; Dölger et al., 2017). By contrast, the toxic species Prymnesium patellifera appears to immobilize or kill large prey before engulfing them by pseudopodia that also form at the posterior pole (Tillmann, 1998). Additionally, putatively flagellated and mixotrophic heterococcolith-bearing species have been described from the fossil record (Gibbs et al<","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"1 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beat the heat: need for research studying plant cell death induced by extreme temperatures

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-03-04 DOI: 10.1111/nph.70045

Joanna Kacprzyk, Paul F. McCabe, Carl K.-Y. Ng

{"title":"Beat the heat: need for research studying plant cell death induced by extreme temperatures","authors":"Joanna Kacprzyk, Paul F. McCabe, Carl K.-Y. Ng","doi":"10.1111/nph.70045","DOIUrl":"https://doi.org/10.1111/nph.70045","url":null,"abstract":"Extreme temperatures surpassing 45°C can cause widespread plant damage and mortality, with severe consequences for ecosystem health, agricultural productivity, and urban greenery, thus negatively impacting human well-being. The global land area experiencing regular heatwaves is increasing, and this trend is expected to continue for the foreseeable future. Despite this alarming scenario, the molecular mechanisms underlying plant thermotolerance and responses to extreme heat-induced damage are not fully understood. As cells are the basic building blocks of the plant, studies at the cellular level are required to elucidate the fine-tuned signaling pathways regulating plant cell death and survival under high heat stress, thereby generating knowledge needed to better understand extreme temperature responses at the whole plant level. Well-established model systems that allow accurate measurement and quantification of stress-induced programmed cell death have a strong potential to enable multifactorial studies, including the use of heat regimes informed by natural settings and combinatorial stress experiments. The knowledge gained as a result can inform the development of effective heat stress mitigation strategies. Studying how plant cells cope with extreme heat is aligned with the One Health approach, several United Nations Sustainable Development Goals, and is, therefore, a research area that demands urgent attention.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"52 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A translocation between chromosome 6 and 8 influences lncRNA_MYB114 and PpRPP13 expression and underpins red leaf trait and powdery mildew resistance in peach

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-03-04 DOI: 10.1111/nph.70028

Shihang Sun, Junren Meng, Wenjun Zhang, Ang Li, Liang Niu, Lei Pan, Wenyi Duan, Jia-Long Yao, Guochao Cui, Zhiqiang Wang, Wenfang Zeng

{"title":"A translocation between chromosome 6 and 8 influences lncRNA_MYB114 and PpRPP13 expression and underpins red leaf trait and powdery mildew resistance in peach","authors":"Shihang Sun, Junren Meng, Wenjun Zhang, Ang Li, Liang Niu, Lei Pan, Wenyi Duan, Jia-Long Yao, Guochao Cui, Zhiqiang Wang, Wenfang Zeng","doi":"10.1111/nph.70028","DOIUrl":"https://doi.org/10.1111/nph.70028","url":null,"abstract":"\u0000<ul>\u0000<li>Red leaf peach has important ornamental value owing to its characteristic leaf coloration. However, this species is highly susceptible to powdery mildew, and the mechanisms of red leaf formation, resistance to powdery mildew, and their relationship remain unclear.</li>\u0000<li>We performed population genetic analyses of red leaf peach, revealing that the translocation of chromosomes 6 and 8 is genetically linked to both the red leaf trait and powdery mildew resistance. Bulk segregant analysis-sequencing, genome resequencing, and expression analysis indicated that the PpMYB114 and the resistance gene PpRPP13 are responsible for the red leaf phenotype and powdery mildew resistance, respectively.</li>\u0000<li>The chromosomal translocation causes a promoter fragment of PpRPP13 on chromosome 8 to integrate into the antisense chain of PpMYB114 on chromosome 6, thereby enhancing the expression of PpMYB114 and inhibiting the expression of PpRPP13. Further, lncRNA-seq identified a new antisense lncRNA, lncRNA_MYB114, which is generated by the translocation and can activate PpMYB114 expression to synthesize anthocyanin. Moreover, the overexpression of PpRPP13 resulted in enhanced resistance to powdery mildew.</li>\u0000<li>In summary, these results revealed the molecular mechanism of a chromosomal translocation altering the expression of PpMYB114 and PpRPP13 to form the red leaf phenotype that is linked to powdery mildew susceptibility.</li>\u0000</ul>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"2 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cucumber mosaic virus 2b directs fibrillarin translocation to plasmodesmata to promote viral movement 黄瓜花叶病毒 2b 引导纤维素转位到质体，促进病毒移动

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-03-03 DOI: 10.1111/nph.70020

Dan Zhang, Haiying Xu, Nam-Hai Chua

{"title":"Cucumber mosaic virus 2b directs fibrillarin translocation to plasmodesmata to promote viral movement","authors":"Dan Zhang, Haiying Xu, Nam-Hai Chua","doi":"10.1111/nph.70020","DOIUrl":"https://doi.org/10.1111/nph.70020","url":null,"abstract":"<h2> Introduction</h2>\u0000Cucumber mosaic virus (CMV) is one of the most widespread and infectious plant viruses affecting over 1200 plant species, including both monocots and dicots (Palukaitis et al., 1992; Mochizuki & Ohki, 2011). The CMV genome consists of three positive-stranded RNAs encoding five proteins: 1a, 2a, 2b, 3a, and coat protein (CP; Jacquemond, 2012). Among them, the multifunctional 2b protein regulates diverse processes throughout the viral life cycle, including viral movement both locally and systemically (Nemes et al., 2014), symptom development (Lewsey et al., 2009), and suppression of RNA silencing as part of the host defense response (Ji & Ding, 2001; Zhang et al., 2006; Zhou et al., 2014).\u0000The 2b proteins of subgroup IA CMV strains, including Fenny Dale (Fny)-CMV strain and Shangdong (SD)-CMV strain, are known to partition between the nucleus and the cytoplasm, yet the biological relevance of such phenomena remains uncertain. Nuclear targeting of 2b proteins from subgroup IA strains is governed by two nuclear localization signals (NLSs), NLS1 and NLS2 (Wang et al., 2004; González et al., 2010; Duan et al., 2012). It was previously shown that NLS mutations impaired 2b's functions in RNA silencing suppression and virus pathogenicity (Lucy et al., 2000; Wang et al., 2004; Lewsey et al., 2009; González et al., 2010). Interestingly, other studies suggested that Fny2b fused with a nuclear export signal, which inhibits its sustained nuclear accumulation, still retains virus silencing suppressor (VSR) activity, suggesting that nuclear localization is not strictly required for this function (González et al., 2012). On the other hand, increasing 2b's nuclear accumulation can reduce its VSR activity while enhancing viral virulence (Du et al., 2014). These findings suggest that the NLS motifs may have broader implications in 2b functionality, potentially beyond its role in VSR activity.\u0000Emerging evidence indicates that the ability of certain viral proteins to form liquid–liquid phase-separated (LLPS) condensates is essential for multiple aspects of the viral lifestyle, including enhancing replication, movement, and host manipulation (Etibor et al., 2021; May, 2024). These condensates act as dynamic compartments, organizing viral and host components to enhance the efficiency of viral gene functions. Liquid–liquid phase-separated-driven interactions between viral proteins and host factors have been shown to facilitate the systemic infection of various plant viruses (Brown et al., 2021). Nevertheless, whether CMV-2b proteins are capable of forming con","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"66 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single-cell transcriptomics

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-03-03 DOI: 10.1111/nph.20446

Rubén Tenorio Berrío, Eline Verhelst, Thomas Eekhout, Carolin Grones, Lieven De Veylder, Bert De Rybel, Marieke Dubois

{"title":"Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single-cell transcriptomics","authors":"Rubén Tenorio Berrío, Eline Verhelst, Thomas Eekhout, Carolin Grones, Lieven De Veylder, Bert De Rybel, Marieke Dubois","doi":"10.1111/nph.20446","DOIUrl":"https://doi.org/10.1111/nph.20446","url":null,"abstract":"Drought stress imposes severe challenges on agriculture by impacting crop performance. Understanding drought responses in plants at a cellular level is a crucial first step toward engineering improved drought resilience. However, the molecular responses to drought are complex as they depend on multiple factors, including the severity of drought, the profiled organ, its developmental stage or even the cell types therein. Thus, deciphering the transcriptional responses to drought is especially challenging. In this study, we investigated tissue-specific responses to mild drought (MD) in young Arabidopsis thaliana (Arabidopsis) leaves using single-cell RNA sequencing (scRNA-seq). To preserve transcriptional integrity during cell isolation, we inhibited RNA synthesis using the transcription inhibitor actinomycin D, and demonstrated the benefits of transcriptome fixation for studying mild stress responses at a single-cell level. We present a curated and validated single-cell atlas, comprising 50 797 high-quality cells from almost all known cell types present in the leaf. All cell type annotations were validated with a new library of reporter lines. The curated data are available to the broad community in an intuitive tool and a browsable single-cell atlas (http://www.single-cell.be/plant/leaf-drought). We show that the mesophyll contains two spatially separated cell populations with distinct responses to drought: one enriched in canonical abscisic acid-related drought-responsive genes, and another one enriched in genes involved in iron starvation responses. Our study thus reveals a dual adaptive mechanism of the leaf mesophyll in response to MD and provides a valuable resource for future research on stress responses.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"35 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intercropping of non-leguminous crops improves soil biochemistry and crop productivity: a meta-analysis

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-02-28 DOI: 10.1111/nph.70037

Muhammad Khashi u Rahman, Zaki Saati-Santamaría, Paula García-Fraile

{"title":"Intercropping of non-leguminous crops improves soil biochemistry and crop productivity: a meta-analysis","authors":"Muhammad Khashi u Rahman, Zaki Saati-Santamaría, Paula García-Fraile","doi":"10.1111/nph.70037","DOIUrl":"https://doi.org/10.1111/nph.70037","url":null,"abstract":"\u0000<ul>\u0000<li>Plant species-rich systems tend to be more productive than depauperate ones. In agroecosystems, increasing crop plant diversity by including legumes often increases soil nitrogen (N) and improves soil fertility; however, such generality in outcomes of non-leguminous crop mixture is unknown.</li>\u0000<li>Here, through a meta-analysis of 174 individual cases, we explored the current global research trend of intercropping of exclusively non-leguminous crops (ICnl) and quantified its effect on agroecosystem productivity key metrics, for example crop plant health, soil chemistry, and microbial community under diverse experimental conditions.</li>\u0000<li>ICnl increased plant biomass and disease suppression and provided a notable yield advantage over monocultures. In addition to phosphorus and potassium, ICnl also increased plant-available soil N, which, along with increased soil microbial abundance, was positively associated with increased soil organic matter. These positive effects were more pronounced in experiments with long duration (> 1 yr), field soil conditions, and soil pH > 7.</li>\u0000<li>ICnl improves several crop productivity metrics, which could augment sustainable crop production, particularly when practiced for a long duration and in alkaline soils.</li>\u0000</ul>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"5 1 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GCN5-related histone acetyltransferase HOOKLESS2 regulates fungal resistance and growth in tomato

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-02-28 DOI: 10.1111/nph.70025

Namrata Jaiswal, Chao-Jan Liao, Ayomi Indika Hewavidana, Tesfaye Mengiste

{"title":"GCN5-related histone acetyltransferase HOOKLESS2 regulates fungal resistance and growth in tomato","authors":"Namrata Jaiswal, Chao-Jan Liao, Ayomi Indika Hewavidana, Tesfaye Mengiste","doi":"10.1111/nph.70025","DOIUrl":"https://doi.org/10.1111/nph.70025","url":null,"abstract":"<h2> Introduction</h2>\u0000Different biological processes are regulated through the activation or repression of gene expression, which in turn occurs through transcriptional and post-transcriptional regulatory networks. Histone modifications are key players in the activation and repression of gene expression underlying various biological processes (Pandey, 2002) as they modulate the accessibility of DNA to enzymes involved in DNA processing. Histone acetyltransferases (HATs) and deacetylases (HDACs) mediate the reversible modifications of histone tails, modulating gene expressions in development and responses to environmental cues. HATs are ‘writer’ proteins that add an acetyl group on histones lysine residues and, generally, cause gene activation (Marmorstein & Roth, 2001; Carrozza et al., 2003; Ma et al., 2013).\u0000GCN5-related N-acetyltransferases (GNATs) contain an N-terminal HAT domain and a C-terminal bromodomain, considered to be a targeting motif (Dhalluin et al., 1999; Ornaghi et al., 1999). GCN5 is the primary HAT that regulates plant gene expression (Servet et al., 2010; Zhou et al., 2017). Mutations or downregulation of Arabidopsis GCN5 increases plant sensitivity to a variety of stresses and causes pleiotropic developmental phenotypes, such as dwarfism, aberrant organ development, and flower organ identity (Kim et al., 2018; Kotak et al., 2018; Poulios & Vlachonasios, 2018). Epigenetic reprogramming through GCN5 promotes competency for shoot regeneration by controlling shoot apical meristem through control of meristem-regulatory genes and promotes callus formation through expression of root stem cell factors (Kumar et al., 2021). GCN5 promotes transcription of WUSCHEL-related homeobox (WOX), SCARECROW (SCR), and PLETHORA (PLT) stem cell regulators through histone acetylation (HAc) at their promoters (Kim et al., 2018).\u0000Histone acetylation is a significant regulatory mechanism in plant gene expression (Yamamuro et al., 2016; Jiang et al., 2020; Ueda & Seki, 2020). It is also associated with memory for systemic acquired resistance (Jaskiewicz et al., 2011; Gkizi et al., 2021). GCN5 complexes with the adaptor proteins alteration/deficiency in activation 2 (ADA2) (Mao et al., 2006), and this complex functions in growth and stress responses (Hu et al., 2015; Zheng et al., 2018; Wang et al., 2019). ADA2b mediates HAc at auxin-responsive genes (Anzola et al., 2010). The transcription factor (TF) bZIP11 interacts with ADA2b and recruits the ADA2b-GCN5 to ","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"35 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant species richness promotes the decoupling of leaf and root defence traits while species-specific responses in physical and chemical defences are rare

IF 8.3 1区生物学

New Phytologist Pub Date : 2025-02-27 DOI: 10.1111/nph.20434

Leonardo Bassi, Justus Hennecke, Cynthia Albracht, Marcel Dominik Solbach, Akanksha Rai, Yuri Pinheiro Alves de Souza, Aaron Fox, Ming Zeng, Stefanie Döll, Van Cong Doan, Ronny Richter, Anja Kahl, Lea Von Sivers, Luise Winkler, Nico Eisenhauer, Sebastian T. Meyer, Nicole M. van Dam, Alexandra Weigelt

引用次数: 0

TIP2-UDT1-OsUPEX1/2 module regulates tapetum development and function in rice

IF 9.4 1区生物学

New Phytologist Pub Date : 2025-02-27 DOI: 10.1111/nph.20435

Ruifeng Wang, Yaqian Sun, Wanlin Liu, Xiaofei Chen, Jie Xu, Zheng Yuan, Wanqi Liang, Dabing Zhang

{"title":"TIP2-UDT1-OsUPEX1/2 module regulates tapetum development and function in rice","authors":"Ruifeng Wang, Yaqian Sun, Wanlin Liu, Xiaofei Chen, Jie Xu, Zheng Yuan, Wanqi Liang, Dabing Zhang","doi":"10.1111/nph.20435","DOIUrl":"https://doi.org/10.1111/nph.20435","url":null,"abstract":"\u0000<ul>\u0000<li>The tapetum in the anther wall is essential for plant fertility, secreting many components essential for pollen development. Development of the tapetum is controlled by multiple transcription factors and signaling pathways. UDT1, TIP2, TDR, and EAT1 constitute a sequential regulatory cascade crucial for tapetal differentiation in rice, but UDT1- and TIP2-dependent regulatory networks, particularly in early anther development, remain largely unknown.</li>\u0000<li>Functional analysis of knockout mutants and spatial–temporal expression analysis demonstrated overlapping expression of TIP2 and UDT1 in the middle layer and tapetum and that the tip2 mutation was epistatic to udt1. Moreover, TIP2 and UDT1 were shown to heterodimerize to activate the expression of downstream genes essential for early anther development.</li>\u0000<li>We identified two genes activated by TIP2-UDT1, OsUPEX1 and OsUPEX2, predicted to encode galactosyltransferases, that were preferentially expressed in the tapetum. Analysis of their single mutants demonstrated their functional redundancy, while the double knockout mutant revealed their critical roles in tapetum development and function, likely in enabling tapetal secretion.</li>\u0000<li>Overall, this study provides insights into the regulation of rice anther development by TIP2 and UDT1 and identifies downstream targets OsUPEX1 and OsUPEX2 essential for tapetum function and rice male fertility.</li>\u0000</ul>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"85 1","pages":""},"PeriodicalIF":9.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0