COmapper: high-resolution mapping of meiotic crossovers by long-read sequencing in Arabidopsis

IF 8.1 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-06-19 DOI:10.1111/nph.70304

Dohwan Byun, Namil Son, Heejin Kim, Jaeil Kim, Jihye Park, Sang-jun Park, Hyein Kim, Jaebeom Kim, Juhyun Kim, Seula Lee, Youbong Hyun, Piotr A. Ziolkowski, Ian R. Henderson, Kyuha Choi

引用次数: 0

Abstract

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比较：通过长读测序对拟南芥减数分裂交叉进行高分辨率定位

减数分裂交叉重组同源染色体之间存在的遗传变异，深刻影响基因组多样性。交叉通常限于每对染色体一到三个事件，它们的分布由染色质可及性和DNA多态性决定。通过高通量短读测序或连锁读测序，可以在植物中生成全基因组交叉图谱。在这里，我们使用长读纳米孔测序技术开发了一个交叉定位管道，COmapper，用于高分辨率定位来自拟南芥材料Col和Ler杂交的F1杂交花粉和F2重组幼苗的DNA杂交。我们通过对拟南芥F2个体的混合DNA进行纳米孔长读测序，并通过短读测序绘制交叉序列，验证了COmapper的高准确性。利用COmapper，我们利用野生型（WT）和交叉提升的recq4a、recq4b突变体的F1杂交花粉和F2幼苗构建了高分辨率的杂交基因组图谱，结果与短读测序结果相当。在WT基因近端启动子上，交叉基因丰富，而在recq4a、recq4b中，交叉基因增加，但被高多态性密度重塑。我们认为，COmapper将广泛适用于探索遗传、表观遗传和环境变化对不同植物物种交叉模式的影响。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.