RABBIT EARS直接调控WOX4转录，抑制拟南芥茎的次生生长。

IF 8.1 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-09-27 DOI:10.1111/nph.70607

Gaurav Thapa Chhetri,Qian Du,Shiqi Zhao,Xia Cui,Liying Qi,Huanzhong Wang

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引用次数: 0

摘要

植物次生生长驱动茎增粗和生物量积累，但其调控机制尚不完全清楚。我们发现了一种新的半显性突变体rbe-d，其特征是形成层细胞显著减少，茎束间区完全没有二次生长。基因克隆实验表明，C2H2锌指转录因子AT5G06070/RABBIT EARS （RBE）的激活与RBE -d表型有关。转基因分析证实，RBE过表达抑制了次生生长，而RBE -2突变体增加了束间形成层衍生区（ICD）的宽度。RBE基因在原形成层和形成层表达。转录组学分析表明，rbe-d突变株的管状细胞分化抑制因子-韧皮部插木质部中枢调控通路（TDIF-PXY）基因受到抑制。生化分析证实RBE直接结合wuschelrelated homeobox （WOX4）启动子，这是一个TDIF-PXY下游的WOX基因，调节形成层细胞增殖。此外，遗传分析证实WOX4在次生生长中对RBE具有上位性。我们的研究结果表明，RBE通过直接抑制WOX4抑制形成层增殖，从而影响次生生长。这些发现为研究拟南芥茎的次生生长调控提供了有价值的新见解。

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RABBIT EARS directly regulates WOX4 transcription and inhibits secondary growth in Arabidopsis stem.

Plant secondary growth drives stem thickening and biomass accumulation, but its regulation is not yet fully understood. We have identified a novel semi-dominant mutant, rbe-d, characterized by a significant reduction in cambium cells and a complete absence of secondary growth in interfascicular regions in the stem. Gene cloning experiments indicated that the activation of the C2H2 zinc finger transcription factor, AT5G06070/RABBIT EARS (RBE), is responsible for the rbe-d phenotype. Transgenic analysis confirmed that overexpression of RBE represses secondary growth, while the rbe-2 mutant increased the width of the interfascicular cambium-derived (ICD) region. The RBE gene is expressed in the procambium and cambium regions. Transcriptomic analysis showed that genes of the tracheary element differentiation inhibitory factor-phloem intercalated with xylem (TDIF-PXY) central regulatory pathway are repressed in the rbe-d mutant plants. Biochemical analyses confirmed that RBE binds directly to the promoter of WUSCHEL-related homeobox (WOX4), a TDIF-PXY downstream WOX gene that regulates cambium cell proliferation. Moreover, genetic analysis confirmed that WOX4 is epistatic to RBE in secondary growth. Our results indicate that RBE inhibits cambium proliferation and thereby impacts secondary growth by directly repressing WOX4. These findings offer valuable new insight into the regulation of secondary growth in the Arabidopsis stem.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.