生物学最新文献

{"title":"Canadian 2021 H-type Bovine Spongiform Encephalopathy case associated with a novel E211K polymorphism in prion protein gene.","authors":"Waqas Tahir, Sandor Dudas, Renee Anderson, Jianmin Yang, Sarah Bogart, Kristina Santiago-Mateo, Yuanmu Fang, Roberta Quaghebeur","doi":"10.1080/19336896.2025.2511933","DOIUrl":"10.1080/19336896.2025.2511933","url":null,"abstract":"<p><p>Bovine Spongiform Encephalopathy (BSE) is a fatal neurodegenerative disease in cattle which can be either classical BSE (C-BSE) or atypical BSE (including H-BSE and L-BSE). Here, we report the results of our analyses of an H-BSE case found in Canada in 2021, indicating restriction of the pathological agent (PrP^Sc) mainly to the central nervous system with no or occasional weak involvement of peripheral tissues. Importantly, a non-synonymous mutation at codon 211 of the <i>PRNP</i> gene was detected and confirmed to be present as a germline mutation. This is the first case of BSE in Canada with a predisposing E211K mutation.</p>","PeriodicalId":54585,"journal":{"name":"Prion","volume":"19 1","pages":"36-49"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12323412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Senecavirus a can replicate in apical-out porcine intestinal organoids and induce stress granules and innate immune response.","authors":"Ying Wang, Zhiying Wei, Jiaxuan Li, Xiaona Wang, Yanping Jiang, Wen Cui, Zhifu Shan, Lijie Tang","doi":"10.1080/21505594.2025.2548623","DOIUrl":"https://doi.org/10.1080/21505594.2025.2548623","url":null,"abstract":"<p><p>Senecavirus A (SVA) causes clinical blistering and ulcerative lesions resembling foot-and-mouth disease (FMD), often with mixed infections that exacerbate the disease and impact pig industry development. SVA has been demonstrated to induce diarrhea, dehydration and mortality in piglets. However, the underlying mechanisms of SVA-related intestinal infections remain underexplored. In this study, a three-dimensional cultured apical-out porcine intestinal organoid model was constructed, comprising a variety of cell types, including intestinal stem cells, enterocytes, goblet cells, proliferative cells, Paneth cells and enteroendocrine cells. The model demonstrated SVA susceptibility in intestinal epithelial cells through cytopathic effects, viral detection, and replication. The results revealed an infection sequence from enterocytes to enteroendocrine cells, Paneth cells and intestinal stem cells and ultimately to proliferating cells, which identified enterocytes as the primary SVA targets. The presence of stress granules was observed at 4 hours post-infection (hpi), with a notable decline over time, reaching near-disappearance at 20 hpi. At this stage, an innate immune response was evident, with significant upregulation of the interferon IFN-α, the interferon-stimulated gene ISG-15, OAS1, OAS2, the signal transducer and activator of transcription 1 (STAT1), the mucosal immunity gene Muc2, and the cytokine IL-6, which appeared to limit further SVA infection. This study elucidates the infection pattern of SVA in intestinal epithelial cells and reveals the mechanism of interaction. It offers insights for controlling secondary infections.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2548623"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRRSV evades innate immune cGAS-STING antiviral function via its Nsp5 to deter STING translocation and activation.","authors":"Yulin Xu, Chenglin Chi, Qihang Xin, Jiang Yu, Yuyu Zhang, Pingping Zhang, Wangli Zheng, Sen Jiang, Wanglong Zheng, Nanhua Chen, Jiaqiang Wu, Jianzhong Zhu","doi":"10.1080/21505594.2025.2548625","DOIUrl":"https://doi.org/10.1080/21505594.2025.2548625","url":null,"abstract":"<p><p>Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is an important pathogen that seriously endangers pig breeding, causing significant economic losses to the global swine industry. Our previous study found that the DNA sensing innate cGAS-STING signaling pathway plays an important role in inducing interferon (IFN) upon PRRSV infection and inhibition of PRRSV replication. However, the mechanism underlying immune evasion by PRRSV remains unclear. In the current study, we found that PRRSV non-structural protein 5 (Nsp5) strongly inhibits the cGAS-STING-IFN antiviral response. Furthermore, we found that Nsp5 interacts with STING, blocking STING transport from the ER to the Golgi apparatus and interfering with STING recruitment of TBK1/IKKε/IRF3. Finally, we demonstrated that the Nsp5 36-47 and 58-67 amino acid regions are critical for inhibiting STING activity and PRRSV replication. This study describes a novel mechanism by which PRRSV suppresses the host innate antiviral response and has implications for our understanding of PRRSV pathogenesis.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2548625"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ABC type fucose operon regulated by XtrSs through CcpA contributes to <i>Streptococcus suis</i> survival in macrophages.","authors":"Song Liang, Yumin Zhang, Shidan Zhang, Qiankun Bai, Xinming Pan, Jianan Liu, Qibing Gu, Huochun Yao, Guangjin Liu","doi":"10.1080/21505594.2025.2553790","DOIUrl":"10.1080/21505594.2025.2553790","url":null,"abstract":"<p><p><i>Streptococcus suis</i> (<i>S. suis</i>), an important zoonotic pathogen, poses a huge threat to the pig industry and human health. The fucose operon (FCS) is found in many bacteria that utilize host glycosylation modifications, contributing to bacterial growth and infection. We previously discovered that the virulence associated streptococcal transcriptional regulator XtrSs could downregulate FCS transcription. In this study, the FCSs of <i>S. suis</i> were classified into ABC and PTS types, and XtrSs indirectly repressed ABC type FCS transcription. Importantly, we found that Catabolite control protein A (CcpA) was the intermediate regulator of XtrSs, but not the FCS upstream regulator FcsR, directly repressing ABC type FCS transcription by binding to a novel Cre site. Interestingly, although ABC type FCS responded to fucose, <i>S. suis</i> failed to proliferate in media with fucose as the sole carbon source. Notably, FCS mutant treated macrophages exhibited decreased fucosyltransferases transcription and impaired AMPK/mTOR signaling to a certain extent, which resulted in increased autophagy processes and ultimately decreasing <i>S. suis</i> survival in macrophages. In conclusion, our findings first confirmed the detailed regulatory mechanism of ABC type FCS and revealed that FCS contributed to the intramacrophage survival of <i>S. suis</i> by inhibiting autophagy processes.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2553790"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12416204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferroptosis and iron-based therapies in <i>Pseudomonas aeruginosa</i> infections: From pathogenesis to treatment.","authors":"Dong-Han Shao, Yu-Feng Yao, Dan-Ni Wang","doi":"10.1080/21505594.2025.2553787","DOIUrl":"10.1080/21505594.2025.2553787","url":null,"abstract":"<p><p><i>Pseudomonas aeruginosa</i> is a globally prevalent multidrug-resistant pathogen that causes severe infections, particularly in immunocompromised individuals. This review focuses on the dual role of iron in <i>P. aeruginosa</i> infections: as a critical nutrient for bacterial growth and as a mediator of host cell ferroptosis, a form of iron-dependent cell death. We summarize how <i>P. aeruginosa</i> manipulates iron metabolism to induce ferroptosis in host cells, thereby promoting its own survival and pathogenicity. Additionally, we explore therapeutic strategies targeting iron metabolism, including interfering with acquisition of iron ions from the environment, disrupting bacterial iron metabolism and iron homeostasis, using ferroptosis inhibitors to suppress host cell ferroptosis, and employing high iron concentrations to induce bacterial ferroptosis. These insights provide innovative approaches to combat drug-resistant <i>P. aeruginosa</i> infections.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2553787"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12416177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative use of gram-positive enhancer matrix particles and affinity peptides in a vaccine against Coxsackievirus B3.","authors":"Shaoju Qian, Ruixue Li, Guanyu Chen, Yinghua Ma, Xuehan Zhang, Zhou Tang, Yihang Song, Zhishan Xu, Zihan Zhang, Yeqing He, Xingyi Zhang, Shuao Lu, Zishan Yang, Xiangfeng Song, Wenfa Yu, Lili Yu","doi":"10.1080/21505594.2025.2481657","DOIUrl":"10.1080/21505594.2025.2481657","url":null,"abstract":"<p><p>Viral myocarditis (VM) is an inflammatory disease posing a serious threat to public health, with various viral pathogens contributing to its pathogenesis. Coxsackievirus B3 (CVB3) is the most frequently implicated causative agent and has been extensively studied because of its high prevalence and severity. No specific therapeutic interventions for VM exist, and vaccine development has encountered substantial challenges. Therefore, we aimed to develop a novel CVB3 mucosal vaccine as a preventive strategy against VM. Gram-positive enhancer matrice (GEM) particles serve as innovative mucosal vaccine adjuvants and antigen delivery systems that enhance antigen immunogenicity by facilitating effective mucosal immune responses. In this study, GEM particle display technology was used to develop two novel CVB3 vaccines: (1) a GEM particle-based vaccine displaying the CVB3 capsid protein VP1 via a PA anchor protein (GEM-PA-VP1), and (2) a GEM particle-based vaccine displaying VP1 via the FcSP peptide (GEM-Fc-VP1). Both GEM-PA-VP1 and GEM-Fc-VP1 vaacines significantly elevated levels of specific IgG, IgG1, IgG2a, sIgA and neutralizing antibodies in a mouse model, along with enhanced secretion of Th1- and Th2-associated cytokines, compared to controls. Notably, GEM-Fc-VP1 demonstrated superior immunogenicity compared with that of GEM-PA-VP1, evidenced by higher antibody titres and cytokine responses. In challenge protection experiments, both vaccines significantly improved survival rates, reduced myocardial enzyme levels, and decreased inflammatory cell infiltration in myocardial tissue, with GEM-Fc-VP1 exhibiting greater efficacy. These findings establish a foundation for the development of a safe and effective CVB3 candidate vaccine and provide novel insights into the potential of peptide-mediated subunit vaccine approaches.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2481657"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12080276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational limitations and future needs to unravel the full potential of 2'-O-methylation and C/D box snoRNAs.","authors":"Christian Ramirez, Elena Perenthaler, Fabio Lauria, Toma Tebaldi, Gabriella Viero","doi":"10.1080/15476286.2025.2506712","DOIUrl":"10.1080/15476286.2025.2506712","url":null,"abstract":"<p><p>This review evaluates the current state of C/D snoRNA databases and prediction tools in relation to 2'-O-methylation (2'-O-Me). It highlights the limitations of existing resources in accurately annotating and predicting guide snoRNAs, particularly for newly identified 2'-O-Me sites. We emphasize the need for advanced computational approaches specifically tailored to 2'-O-Me to enable the discovery and functional analysis of snoRNAs. Given the growing importance of 2'-O-Me in areas such as cancer epitranscriptomics, ribosome biogenesis, and heterogeneity, existing tools remain inadequate. As 2'-O-Me gains recognition as a potential biomarker and therapeutic target, more sophisticated methods are urgently needed to improve snoRNA annotation and prediction, facilitating biomedical advancements.</p>","PeriodicalId":21351,"journal":{"name":"RNA Biology","volume":" ","pages":"1-11"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the versatility of <i>Drosophila melanogaster</i> as a model organism in biomedical research: a comprehensive review.","authors":"Ayomide Victor Atoki, Patrick Maduabuchi Aja, Tijjani Salihu Shinkafi, Erick Nyakundi Ondari, Adekunle Ismahil Adeniyi, Ilemobayo Victor Fasogbon, Reuben Samson Dangana, Umar Uthman Shehu, Akinpelumi Akin-Adewumi","doi":"10.1080/19336934.2024.2420453","DOIUrl":"https://doi.org/10.1080/19336934.2024.2420453","url":null,"abstract":"<p><p><i>Drosophila melanogaster</i> is a highly versatile model organism that has profoundly advanced our understanding of human diseases. With more than 60% of its genes having human homologs, <i>Drosophila</i> provides an invaluable system for modelling a wide range of pathologies, including neurodegenerative disorders, cancer, metabolic diseases, as well as cardiac and muscular conditions. This review highlights key developments in utilizing <i>Drosophila</i> for disease modelling, emphasizing the genetic tools that have transformed research in this field. Technologies such as the GAL4/UAS system, RNA interference (RNAi) and CRISPR-Cas9 have enabled precise genetic manipulation, with CRISPR-Cas9 allowing for the introduction of human disease mutations into orthologous <i>Drosophila</i> genes. These approaches have yielded critical insights into disease mechanisms, identified novel therapeutic targets and facilitated both drug screening and toxicological studies. Articles were selected based on their relevance, impact and contribution to the field, with a particular focus on studies offering innovative perspectives on disease mechanisms or therapeutic strategies. Our findings emphasize the central role of <i>Drosophila</i> in studying complex human diseases, underscoring its genetic similarities to humans and its effectiveness in modelling conditions such as Alzheimer's disease, Parkinson's disease and cancer. This review reaffirms <i>Drosophila</i>'s critical role as a model organism, highlighting its potential to drive future research and therapeutic advancements.</p>","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2420453"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of AgNPs and zileuton on PCOS via ferroptosis and inflammation mitigation.","authors":"Amira K Eltokhy, Rehab Ahmed Ahmed El-Shaer, Omnia Safwat El-Deeb, Eman E Farghal, Rowida Raafat Ibrahim, Rasha Elesawy, Marwa Mahmoud Awad, Radwa Ismail, Shaimaa M Motawea, Doaa Shatat, Yasser Mostafa Hafez, Hend Ahmed El Hanafy, Marwa Mohamed Atef","doi":"10.1080/13510002.2024.2445398","DOIUrl":"https://doi.org/10.1080/13510002.2024.2445398","url":null,"abstract":"<p><strong>Background: </strong>The most prevalent endocrine disorder affecting women is PCOS. Programmed death of ovarian cells has yet to be elucidated. Ferroptosis is a kind of iron-dependent necrosis featured by significantly Fe⁺²-dependent lipid peroxidation. The ongoing study aimed to reinforce fertility by combining therapy with AgNPs and (Zileuton) in PCOS rats' model.</p><p><strong>Methods: </strong>The study included 75 adult female rats divided into 5 groups; control, PCOS, PCOS treated with AgNPs, PCOS treated with Zileuton, and PCOS group treated with AgNPs and Zileuton. The study investigated the anti-ferroptotic, anti-inflammatory, antioxidant, antiapoptotic, histopathological and immunohistochemical examinations of COX-2 and VEGF.</p><p><strong>Results: </strong>The combination of AgNPs and Zileuton showed significant reduction of inflammatory mediators (IL-6, TNF-α, NFk-B) compared with diseased group (<i>P</i>-<i>value</i> < 0.05), regression of ferroptosis marks (Panx1 and TLR4 expression, Fe⁺² levels) compared with diseased group (<i>P</i>-<i>value</i> < 0.05), depression of apoptotic marker caspase 3 level compared with diseased animals (<i>P</i>-value < 0.05), depression of MDA level, elevation of HO-1, GPx4 activity, and reduction of Cox2 and VEGF as compared with the diseased, AgNPs or zileuton-treated groups (<i>P</i>-value < 0.05).</p><p><strong>Conclusion: </strong>The study showed that the combination of AgNPs and zileuton guards against, inflammation, apoptosis, and ferroptosis in PCO.</p>","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"30 1","pages":"2445398"},"PeriodicalIF":5.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Staphylococcus aureus nt5</i> gene mutation through CRISPR RNA-guided base editing weakens bacterial virulence and immune evasion.","authors":"Xinpeng Liu, Lan Huang, Yang Ye, Haiyi Wang, Min Tang, Fuqiang He, Zijing Xia, Shi Deng, Peng Zhang, Ruiwu Dai, Shufang Liang","doi":"10.1080/21505594.2025.2451163","DOIUrl":"10.1080/21505594.2025.2451163","url":null,"abstract":"<p><p>The resistance of commonly used clinical antibiotics, such as daptomycin (DAP), has become increasingly serious in the fight against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) infection. It is essential to explore key pathogenicity-driven genes/proteins in bacterial infection and antibiotics resistance, which contributes to develop novel therapeutic strategies against <i>S. aureus</i> infections. The <i>nt5</i> gene of <i>S. aureus</i>, encoding 5'-nucleotidase (NT5), is nearly unknown for its function in drug resistance and bacterial infection. Herein, to reveal <i>nt5</i> gene role in drug resistance and infection ability of <i>S. aureus</i>, we performed <i>nt5</i>^C166T gene mutation using a clustered regulatory interspaced short palindromic repeat ribonucleic acid (RNA)-guided base editing system to investigate the lose-of-function of NT5 protein. Subsequent transcriptome sequencing of the mutant strain revealed that <i>nt5</i> inactivation caused changes in cell membrane integrity and inhibited nucleotide metabolism, suggesting the <i>nt5</i> gene may be involved in bacterial drug resistance and virulence. The mutant strain exhibited enhanced tolerance to DAP treatment by attenuating cell membrane potential dissipation and slowing deoxyribonucleic acid release. Moreover, the <i>nt5</i> mutation alleviated abscess degree of mouse kidneys caused by <i>S. aureus</i> infection byreducing the expression of IL-1β, IL-6, and IL-18. The <i>nt5</i> mutant strain was easily swallowed by host immune cells, resulting in weak bacterial toxicity of the <i>S. aureus</i> mutant in the bacterial infection process. In summary, <i>nt5</i> gene mutation confers tolerance to DAP and a lower bacterial capacity to form kidney abscesses through phagocytosis of host immune cells, which indicates the targeted inhibition of NT5 protein would offer a potential new therapeutic strategy against <i>S. aureus</i> infection.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2451163"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}