生物学最新文献

{"title":"Sleep timing in flies from \"adolescence\" to adulthood.","authors":"Esther D Domenie, Paola Cusumano, Sara Montagnese, Rodolfo Costa","doi":"10.1080/19336934.2024.2448022","DOIUrl":"https://doi.org/10.1080/19336934.2024.2448022","url":null,"abstract":"<p><p>The aim of the present study was to assess sleep timing in <i>Drosophila melanogaster</i> at different ages, within the setting of an enforced schedule of varying light-dark stimuli, simulating light exposure variations between four typical office <i>working days</i> and one <i>free day</i> spent outside by a human, for a total of 30 days. Locomotor activity recording started when male flies were 3 days old. Flies exhibited a bimodal activity pattern, with a morning and an evening peak, and clear anticipation of the <i>lights on</i> and <i>lights off</i> transitions. From experimental day 10 (i.e. 12-day-old flies) onwards, a decrease in activity counts/increase in sleep amount were observed. On <i>free days</i>, a rise in activity counts and a reduction in sleep amount during the <i>lights on</i> interval was observed and was also present, albeit less obvious, on the subsequent <i>working day</i> during the <i>lights off</i> interval. A progressive delay in sleep onset was observed in the first days of the experiment, peaking on day 4 (i.e. 6-day-old flies), after which sleep onset timing gradually advanced. A delay in sleep offset was also observed for the first 13 days of the experiment, after which sleep offset stabilized. In conclusion, 'adolescent' flies exhibited changes in sleep timing that were reminiscent of those of human adolescents.</p>","PeriodicalId":12128,"journal":{"name":"Fly","volume":"19 1","pages":"2448022"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoregulation of the biosynthetic activity of fungus <i>Inonotus obliquus</i> using colloidal solutions of biogenic metal nanoparticles and low-intensity laser radiation.","authors":"Oksana Mykchaylova, Anatoliy Negriyko, Nadiia Matvieieva, Kostiantyn Lopatko, Natalia Poyedinok","doi":"10.1080/21655979.2025.2458371","DOIUrl":"10.1080/21655979.2025.2458371","url":null,"abstract":"<p><p>This article presents new data on the integrated use of colloidal solutions of nanoparticles and low-intensity laser radiation on the biosynthetic activity of the medicinal mushroom <i>Inonotus obliquus in vitro</i>. Traditional mycological methods, colloidal solutions of biogenic metals, and unique photobiological methods have also been used. It was found that colloidal solutions of nanoparticles of all metals used increased the growth characteristics of <i>I. obliquus</i> (55-60%), while irradiation of the fungal inoculum with laser light in a medium with nanoparticles reduced the growth activity of <i>I. obliquus</i> mycelia by 12.3-35.4%. Silver nanoparticles (AgNPs) in a nutrient medium suppressed the biosynthesis of extracellular polysaccharides, whereas laser irradiation in the same medium increased the synthesis of intracellular polysaccharides by 9.7 times. Magnesium nanoparticles (MgNPs) and iron nanoparticles (FeNPs) inhibited the synthesis of intracellular polysaccharides in the mycelial mass of <i>I. obliquus</i>. At the same time, laser irradiation of the inoculum with MgNPs, on the contrary, induced a sharp increase in the amount of polysaccharides in the culture liquid (20 times). Treatment of the inoculum in a medium with nanoparticles with a laser caused an intensification of the synthesis of flavonoids in the mycelial mass and an increase in the synthesis of melanin pigments (25-140%). The results obtained suggest the possibility of the complex use of colloidal solutions of Fe, Ag, and Mg nanoparticles and low-intensity laser radiation as environmentally friendly factors for regulating biosynthetic activity in the biotechnology of cultivating the valuable medicinal mushroom <i>I. obliquus</i>.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2458371"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11776471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbial dysbiosis associated to diarrheic irritable bowel syndrome can be efficiently simulated in the Mucosal ARtificial COLon (M-ARCOL).","authors":"Ophélie Uriot, Charlotte Deschamps, Julien Scanzi, Morgane Brun, Nicolas Kerckhove, Christian Dualé, Elora Fournier, Claude Durif, Sylvain Denis, Michel Dapoigny, Philippe Langella, Monique Alric, Lucie Etienne-Mesmin, Blanquet-Diot Stéphanie","doi":"10.1080/21655979.2025.2458362","DOIUrl":"10.1080/21655979.2025.2458362","url":null,"abstract":"<p><p>Irritable bowel syndrome (IBS) is a common chronic gastrointestinal disorder, with diarrhea-predominant IBS (IBS-D) as the most frequent subtype. The implication of gut microbiota in the disease's etiology is not fully understood. <i>In vitro</i> gut systems can offer a great alternative to <i>in vivo</i> assays in preclinical studies, but no model reproducing IBS-related dysbiotic microbiota has been developed. Thanks to a large literature review, a new Mucosal ARtifical COLon (M-ARCOL) adapted to IBS-D physicochemical and nutritional conditions was set-up. To validate the model and further exploit its potential in a mechanistic study, <i>in vitro</i> fermentations were performed using bioreactors inoculated with stools from healthy individuals (<i>n</i> = 4) or IBS-D patients (<i>n</i> = 4), when the M-ARCOL was set-up under healthy or IBS-D conditions. Setting IBS-D parameters in M-ARCOL inoculated with IBS-D stools maintained the key microbial features associated to the disease <i>in vivo</i>, validating the new system. In particular, compared to the healthy control, the IBS-D model was characterized by a decreased bacterial diversity, together with a lower abundance of <i>Rikenellaceae</i> and <i>Prevotellaceae</i>, but a higher level of <i>Proteobacteria</i> and <i>Akkermansiaceae</i>. Of interest, applying IBS-D parameters to healthy stools was not sufficient to trigger IBS-D dysbiosis and applying healthy parameters to IBS-D stools was not enough to restore microbial balance. This validated IBS-D colonic model can be used as a robust <i>in vitro</i> platform for studies focusing on gut microbes in the absence of the host, as well as for testing food and microbiota-related interventions aimed at personalized restoration of gut microbiota eubiosis.</p>","PeriodicalId":8919,"journal":{"name":"Bioengineered","volume":"16 1","pages":"2458362"},"PeriodicalIF":4.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11796540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of HIV-1 subtypes and drug resistance mutations in people who inject drugs in Aizawl and nearby districts of Mizoram, India.","authors":"Komal Raskar, Lal Thlengliani, Andrew Lalbiaknunga, Dipali Kale, Ajit Patil, Pragati Chavan, Richard Clr Hluna, Samiran Panda, Swarali Kurle","doi":"10.1080/21505594.2025.2478074","DOIUrl":"10.1080/21505594.2025.2478074","url":null,"abstract":"<p><p>Over the last decade, there has been a steady increase in HIV-1 prevalence in Mizoram, India. Importantly, this increase in HIV-1 prevalence is not only limited to the key population groups such as female sex workers (FSWs) or people who inject drugs (PWID), and has been witnessed in general population as well. Injecting drug use has long been one of the key drivers of HIV-1 epidemic across the north-eastern states of India. In this study, using HIV-1 pol gene region sequences from Aizawl and adjoining districts, we examined the HIV-1 subtypes, recombinant forms, drug resistance mutations and also the spatiotemporal dynamics of the potential unique recombinant forms. In our dataset, the dominant subtype was HIV-1 subtype C (94.91%). We could also identify the presence of CRF01_AE (1.69%) and BC recombinant forms (3.39%). Drug resistance mutation analysis revealed that resistance against non-nucleoside reverse transcriptase inhibitors was most common in the sequences having any resistance mutations. Evolutionary analysis of unique BC recombinants estimated the most recent common ancestor of these sequences around 2004-2005 and them having an ancestry of United States of America (USA) origin.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2478074"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIRT1/DNMT3B-mediated epigenetic gene silencing in response to phytoestrogens in mammary epithelial cells.","authors":"Yuexi Ma, Cayla Boycott, Jiaxi Zhang, Rekha Gomilar, Tony Yang, Barbara Stefanska","doi":"10.1080/15592294.2025.2473770","DOIUrl":"10.1080/15592294.2025.2473770","url":null,"abstract":"<p><p>We performed an integrated analysis of genome-wide DNA methylation and expression datasets in normal cells and healthy animals exposed to polyphenols with estrogenic activity (i.e. phytoestrogens). We identified that phytoestrogens target genes linked to disrupted cellular homeostasis, e.g. genes limiting DNA break repair (<i>RNF169</i>) or promoting ribosomal biogenesis (<i>rDNA</i>). Existing evidence suggests that DNA methylation may be governed by sirtuin 1 (SIRT1) deacetylase via interactions with DNA methylating enzymes, specifically DNMT3B. Since SIRT1 was reported to be regulated by phytoestrogens, we test whether phytoestrogens suppress genes related to disrupted homeostasis via SIRT1/DNMT3B-mediated transcriptional silencing. Human MCF10A mammary epithelial cells were treated with phytoestrogens, pterostilbene (PTS) or genistein (GEN), followed by analysis of cell growth, DNA methylation, gene expression, and SIRT1/DNMT3B binding. SIRT1 occupancy at the selected phytoestrogen-target genes, <i>RNF169</i> and <i>rDNA</i>, was accompanied by consistent promoter hypermethylation and gene downregulation in response to GEN, but not PTS. GEN-mediated hypermethylation and SIRT1 binding were linked to a robust DNMT3B enrichment at <i>RNF169</i> and <i>rDNA</i> promoters. This was not observed in cells exposed to PTS, suggesting a distinct mechanism of action. Although both SIRT1 and DNMT3B bind to <i>RNF169</i> and <i>rDNA</i> promoters upon GEN, the two proteins do not co-occupy the regions. Depletion of SIRT1 abolishes GEN-mediated decrease in <i>rDNA</i> expression, suggesting SIRT1-dependent epigenetic suppression of <i>rDNA</i> by GEN. These findings enhance our understanding of the role of SIRT1-DNMT3B interplay in epigenetic mechanisms mediating the impact of phytoestrogens on cell biology and cellular homeostasis.</p>","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"20 1","pages":"2473770"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A promising endeavor against human cytomegalovirus: Predominant epitopes-based recombinant subunit vaccine RH<i>Ec</i>^{IE1/pp65/pp150}.","authors":"Zonghui Li, Shasha Jiang, Wenxuan Liu, Xiaoli Yang, Fengjun Liu, Xu Li, Jun Li, Meng Yu, Zhun Wei, Bin Wang, Dongmeng Qian","doi":"10.1080/21505594.2025.2497903","DOIUrl":"https://doi.org/10.1080/21505594.2025.2497903","url":null,"abstract":"<p><p>Human cytomegalovirus (HCMV) is widespread in the population, typically remaining latent. However, it can cause severe morbidity and mortality in transplant patients and immunodeficient individuals. Currently, there is no approved vaccine against HCMV. This study used immunoinformatics methods to predict the predominant T and B-cell epitopes of three key HCMV proteins, including phosphoprotein 65 (pp65), pp150, and immediate-early protein 1 (IE1). Subsequently, we synthesized a recombinant subunit vaccine (RH<i>Ec</i>^{IE1/pp65/pp150}) from <i>Escherichia coli</i>, comprising RH<i>Ec</i>-1 and RH<i>Ec</i>-2. We observed that the RH<i>Ec</i>^{IE1/pp65/pp150} vaccine exhibited high safety and immunogenicity in mice, enhancing a significant upregulation of CD80, CD86, CD40, and MHCII on dendritic cells and macrophages. Additionally, the vaccine activated innate immune responses through the NF-κB signalling pathway, triggering CD4⁺ and CD8⁺T cells to secrete tumour necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-2, directing the T-cell response towards Th1. Moreover, it stimulated CD4⁺T cells to secrete IL-4, IL-6, and IL-10, promoting B-cell immunity. Furthermore, the RH<i>Ec</i>^{IE1/pp65/pp150} vaccine induced the formation of abundant memory cells and high levels of neutralizing antibody titres, conducive to providing long-lasting protection. Taken together, the RH<i>Ec</i>^{IE1/pp65/pp150} vaccine is a promising endeavour against HCMV, and these findings contribute valuable insights to the development of HCMV vaccine candidates.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2497903"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative splicing of the Snap23 microexon is regulated by MBNL, QKI, and RBFOX2 in a tissue-specific manner and is altered in striated muscle diseases.","authors":"Gabrielle M Gentile, R Eric Blue, Grant A Goda, Bryan B Guzman, Rachel A Szymanski, Eunice Y Lee, Nichlas M Engels, Emma R Hinkle, Hannah J Wiedner, Aubriana N Bishop, Jonathan T Harrison, Hua Zhang, Xander H T Wehrens, Daniel Dominguez, Jimena Giudice","doi":"10.1080/15476286.2025.2491160","DOIUrl":"https://doi.org/10.1080/15476286.2025.2491160","url":null,"abstract":"<p><p>The reprogramming of alternative splicing networks during development is a hallmark of tissue maturation and identity. Alternative splicing of microexons (small, genomic regions ≤ 51 nucleotides) functionally regulate protein-protein interactions in the brain and is altered in several neuronal diseases. However, little is known about the regulation and function of alternatively spliced microexons in striated muscle. Here, we investigated alternative splicing of a microexon in the synaptosome-associated protein 23 (<i>Snap23</i>) encoded gene. We found that inclusion of this microexon is developmentally regulated and tissue-specific, as it occurs exclusively in adult heart and skeletal muscle. The alternative region is highly conserved in mammalian species and encodes an in-frame sequence of 11 amino acids. Furthermore, we showed that alternative splicing of this microexon is mis-regulated in mouse models of heart and skeletal muscle diseases. We identified the RNA-binding proteins (RBPs) quaking (QKI) and RNA binding fox-1 homolog 2 (RBFOX2) as the primary splicing regulators of the Snap23 microexon. We found that QKI and RBFOX2 bind downstream of the Snap23 microexon to promote its inclusion, and this regulation can be escaped when the weak splice donor is mutated to the consensus 5' splice site. Finally, we uncovered the interplay between QKI and muscleblind-like splicing regulator (MBNL) as an additional, but minor layer of Snap23 microexon splicing control. Our results are one of the few reports detailing microexon alternative splicing regulation during mammalian striated muscle development.</p>","PeriodicalId":21351,"journal":{"name":"RNA Biology","volume":"22 1","pages":"1-20"},"PeriodicalIF":3.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interplay of <i>Mycobacterium abscessus</i> and <i>Pseudomonas aeruginosa</i> in experimental models of coinfection: Biofilm dynamics and host immune response.","authors":"Víctor Campo-Pérez, Esther Julián, Eduard Torrents","doi":"10.1080/21505594.2025.2493221","DOIUrl":"https://doi.org/10.1080/21505594.2025.2493221","url":null,"abstract":"<p><p>The incidence of infection by nontuberculous mycobacteria, mainly <i>Mycobacterium abscessus</i>, is increasing in patients with cystic fibrosis and other chronic pulmonary diseases, leading to an accelerated lung function decline. In most cases, <i>M. abscessus</i> coinfects <i>Pseudomonas aeruginosa</i>, the most common pathogen in these conditions. However, how these two bacterial species interact during infection remains poorly understood. This study explored their behaviour in three relevant pathogenic settings: dual-species biofilm development using a recently developed method to monitor individual species in dual-species biofilms, coinfection in bronchial epithelial cells, and <i>in vivo</i> coinfection in the <i>Galleria mellonella</i> model. The results demonstrated that both species form stable mixed biofilms and reciprocally inhibit single-biofilm progression. Coinfections in bronchial epithelial cells significantly decreased cell viability, whereas in <i>G. mellonella</i>, coinfections induced lower survival rates than individual infections. Analysis of the immune response triggered by each bacterium in bronchial epithelial cell assays and <i>G. mellonella</i> larvae revealed that <i>P. aeruginosa</i> induces the overexpression of proinflammatory and melanization cascade responses, respectively. In contrast, <i>M. abscessus</i> and <i>P. aeruginosa</i> coinfection significantly inhibited the immune response in both models, resulting in worse consequences for the host than those generated by a single <i>P. aeruginosa</i> infection. Overall, this study highlights the novel role of <i>M. abscessus</i> in suppressing immune responses during coinfection with <i>P. aeruginosa</i>, emphasizing the clinical implications for the management of cystic fibrosis and other pulmonary diseases. Understanding these interactions could inform the development of new therapeutic strategies to mitigate the severity of coinfections in vulnerable patients.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2493221"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA transcriptome analysis of rainbow trout (<i>Oncorhynchus mykiss</i>) skin infected with IHNV reveals that lncRNA SARL/miR-205-z/<i>SOCS3</i> axis negatively regulates antiviral immunity mechanisms.","authors":"Lu Zhao, Jinqiang Huang, Yongjuan Li, Shenji Wu","doi":"10.1080/21505594.2025.2486990","DOIUrl":"https://doi.org/10.1080/21505594.2025.2486990","url":null,"abstract":"<p><p>Long non-coding RNAs (lncRNAs) are new gene regulators involved in various biological processes. However, the regulatory effect of lncRNA on the rainbow trout (<i>Oncorhynchus mykiss</i>) antiviral immune response has not been reported. Here, we measured lncRNA profiles at 48 hpi compared to the control group, expression levels of lncRNA, miRNA, and gene, and lncRNA SARL/miR-205-z/<i>SOCS3</i> functions after rainbow trout skin infected with infectious haematopoietic necrosis virus (IHNV) by RNA-seq, qRT-PCR, and overexpression and inhibition assays. Transcriptome analysis identified twelve upregulated and four downregulated DElncRNAs. Twelve key immune-related competing endogenous RNA (ceRNA) networks were identified, and the target genes were enriched in the TLR, RLR, NLR, and p53 signalling pathways. Expression patterns suggested that changes in lncRNA SARL, miR-205-z, and <i>SOCS3</i> expression presented a ceRNA regulatory relationship. Further studies demonstrated that the lncRNA SARL was a ceRNA of <i>SOCS3</i> by sponging miR-205-z <i>in vitro</i>, thereby playing a negative regulatory role in the antiviral immune response of rainbow trout. We also found that miR-205-z was a positive regulator of rainbow trout liver cell proliferation, and this effect could be reversed by <i>SOCS3</i>. <i>In vivo</i>, <i>SOCS3</i> expression significantly increased after antagomiR-205-z injection. Furthermore, <i>SOCS3</i> overexpression significantly promoted the replication of IHNV. This study provides fundamental data for disease resistance breeding and targeted drug therapy in rainbow trout.</p>","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2486990"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144037274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Update on the correlation between mitochondrial function and osteonecrosis of the femoral head osteocytes.","authors":"Chengming Li, Hangyu Ji, Suyang Zhuang, Xinhui Xie, Daping Cui, Cong Zhang","doi":"10.1080/13510002.2025.2491846","DOIUrl":"https://doi.org/10.1080/13510002.2025.2491846","url":null,"abstract":"<p><p>Mitochondrial health is maintained in a steady state through mitochondrial dynamics and autophagy processes. Recent studies have identified healthy mitochondria as crucial regulators of cellular function and survival. This process involves adenosine triphosphate (ATP) synthesis by mitochondrial oxidative phosphorylation (OXPHOS), regulation of calcium metabolism and inflammatory responses, and intracellular oxidative stress management. In the skeletal system, they participate in the regulation of cellular behaviors and the responses of osteoblasts, osteoclasts, chondrocytes, and osteocytes to external stimuli. Indeed, mitochondrial damage or dysfunction occurs in the development of a few bone diseases. For example, mitochondrial damage may lead to an imbalance in osteoblasts and osteoclasts, resulting in osteoporosis, osteomalacia, or poor bone production, and chondrocyte death and inflammatory infiltration in osteoarthritis are the main causes of cartilage degeneration due to mitochondrial damage. However, the opposite exists for osteosarcoma, where overactive mitochondrial metabolism is able to accelerate the proliferation and migration of osteosarcoma cells, which is a major disease feature. Bone is a dynamic organ and osteocytes play a fundamental role in all regions of bone tissue and are involved in regulating bone integrity. This review examines the impact of mitochondrial physiological function on osteocyte health and summarizes the microscopic molecular mechanisms underlying its effects. It highlights that targeted therapies focusing on osteocyte mitochondria may be beneficial for osteocyte survival, providing a new insight for the diagnosis, prevention, and treatment of diseases associated with osteocyte death.</p>","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"30 1","pages":"2491846"},"PeriodicalIF":5.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}